?2440409 Summary - Canadian Patents Database (2024)

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CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
A CRYSTAL OF BACTERIAL CORE RNA POLYMERASE WITH RIFAMPICIN
AND METHODS OF USE THEREOF
FIELD OF THE INVENTION
s The present invention provides a crystal of a binding complex between
rifampicin
and a bacterial core RNA polymerase from T7ZenTnus aquaticus. The three-
dimensional
structural information is included in the invention. The present invention
provides
procedures for identifying agents that can inhibit bacterial cell growth
through the use of
rational drug design predicated on the crystallographic data.
1 o BACKGROUND OF THE INVENTION
RNA in all cellular organisms is synthesized by a complex molecular machine,
the
DNA-dependent RNA polymerase (RNAP). In its simplest bacterial form, the
enzyme
comprises at least 4 subunits with a total molecular mass of around 400 kDa.
The
eukaryotic enzymes comprise upwards of a dozen subunits with a total molecular
mass of
is around 500 kDa. The essential core component of the RNAP (subunit
composition a2[i/3'~)
is evolutionarily conserved from bacteria to man [Archambault and Friesen,
Micf-obiological Reviews, 57:703-724 (1993)]. Sequence homologies point to
structural
and functional homologies, making the simpler bacterial RNAPs excellent model
systems
for understanding the multisubunit cellular RNAPs in general.
a o The basic elements of the transcription cycle were elucidated through
study of the
prokaryotic system. In this cycle, the RNAP, along with other factors, locates
specific
sequences called promoters within the double-stranded DNA, forms the open
complex by
melting a portion of the DNA surrounding the transcription start site,
initiates the synthesis
of an RNA chain, and elongates the RNA chain completely processively while
translocating
itself and the melted transcription bubble along the DNA template. Finally it
releases itself
and the completed transcript from the DNA when a specific termination signal
is
encountered. The current view is that the transcribing RNAP contains sites for
binding the
DNA template as well as forming and maintaining the transcription bubble,
binding the
RNA transcript, and binding the incoming nucleotide-triphosphate substrate.
3 o From the initial indications of DNA-dependent RNAP activity from a number
of
systems, [Weirs and Gladstone, J. Arn. Chem. Soc., 81:4118-4119 (1959)];
Hurwitz et al.,
Biochefn. Bioplays. Res. Cofnmun., 3:15 (1960); Stevens, Biochena. Biophys.
Res. Commun.,
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CA 02440409 2003-09-09
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3:92 (1960); Huang et al., Bioclaena. BioplZys. Res. Cornmun., 3:689 (1960);
and Weiss and
Nakamoto, J. Biol. ClaerrZ., 236:PC 19 (1961)], and the isolation of the RNAP
enzyme from
bacterial sources [Chamberlin and Berg, Proc. Natl. Acad. Sci. USA, 48:81-94
(1962)], a
wealth of biochemical, biophysical, and genetic information has accumulated on
RNAP and
s its complexes with nucleic acids and accessory factors. Nevertheless, the
enzyme itself, in
terms of its structure/function relationship, remains a black box. An
essential step towards
understanding the mechanism of transcription and its regulation is to
determine
three-dimensional structures of RNAP and its complexes with DNA, RNA, and
regulatory
factors [von Hippel et al., Annual reviews ofBioclZemistry, 53:389-446 (1984);
Erie et al.,
i o ATanual Review of Bioplaysics & Biomolecular Structure, 21:379-415 (1992);
Sentenac et
al., Transcriptional Regulatioya, in Cold Spring Harbor Laboratory 27-54, Cold
Spring
Harbor, eds. McKnight and Yamamoto (1992); Gross et al., Philosophical
Transactions of
tlae Royal Society ofLofadon - Series B:Biological Sciences, 351:475-482
(1996); and
Nudler, J. Mol. Biol., 288:1-12 (1999)].
i5 The key feature of low-resolution structures of bacterial and eukaryotic
RNAPs,
provided by electron crystallography, is a thumb-like projection surrounding a
groove or
channel that is an appropriate size for accommodating double-helical DNA
[Durst et al.,
Nature, 340:730-732 (1989); Durst et al., Cell, 66:121-128 (1991); Schultz et
al., EMBO J.,
12:2601-2607 (1993); Polyakov et al., Cell, 83:365-373 (1995); Durst et al.,
J. Structural
2 o Biol., 124:115-122 (1998); and Durst et al., Cold Spring Harbor Symp.
Quant. Biol.,
63:269-276 (1998)].
Bacterial infections remain among the most common and deadly causes of human
disease. Infectious diseases are the third leading cause of death in the
United States and the
leading cause of death worldwide [Binder et al., Science 284:1311-1313
(1999)]. More
25 particularly, each year there are 8-10 million new cases of tuberculosis
(TB). TB is the
leading cause of death in adults by an infectious agent [Raviglioni et al.,
JAMA
273:220-226 (1995); Shinnick, Cuf°rent Topics in Micf-obiol. Inamunol.,
Springer-Verlag
Berlin Heidelberg, New York (1996)] and is in near epidemic proportions in
some parts of
the world. Indeed, the World Health Organization declared TB to be a global
public health
s o emergency due to the rapid increase in mufti-drug resistant strains of
Mycobateriurn
tuberculosis [Raviglioni et al., JAMA 273:220-226 (1995)].
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CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
Rifampicin (Rif) [Sensi, Antibiot.Ann 1959-1960, 262-270 (1960); Sensi et al.,
Rev.Infect.Dis., 5 Supp.3:402-406 (1983)] is one of the most potent and broad-
spectrum
antibiotics against bacterial pathogens and is a key component of anti-TB
therapy. °The
introduction of rifampicin in 1968 greatly shortened the duration of
chemotherapy necessary
s for successful treatment. Rifampicin diffuses freely into tissues, living
cells, and bacteria,
making it extremely effective against intracellular pathogens like M.
tuberculosis [Shinnick,
Current Topics ifa Microbiol. Inamunol., Springer-Verlag Berlin Heidelberg,
New York
(1996)]. However, bacteria develop resistance to rifampicin with high
frequency, which has
led the medical community in the United States to commit to a voluntary
restriction of its
s o use for treatment of TB or emergencies. ~ '
The bactericidal activity of rifampicin stems from its high-affinity binding
to, and
inhibition of, the bacterial DNA-dependent RNA polymerase [Hartmann et al.,
Biochirn.Biophys. Acta 145:843-844 (1967)]. Mutations conferring rifampicin
resistance
(Rife) map almost exclusively to the rpoB gene (encoding the RNAP (3 subunit)
in every
15 organism tested, including E. coli [Ezekiel and Hutchins, Nature London
220:276-277(1968); Heil and Zillig, FEBSLett. 11:165-168 (1970); Wehrli et
al.,
Biochem.Biophysic.Res.Comna., 32:284-288 (1968) and M. tuberculosis [Heep et
al.,
Antimicrob.Agents Chemotherap.44:1075-1077 (2000); Ramaswamy and Musser,
Tubercle
and Lung Disease 79:3-29 (1998)]. Comprehensive genetic analyses have provided
z o molecular details of amino acid alterations in (i subunit conferring Rif
(see Fig. 1) [Jin and
Gross, J.Molec.Biol, 202:45-58 1988; Lisitsyn et al., Bioorg Khim 10:127-128
(1984);
Lisitsyn et al., Molec.Gen.Geyaet., 196:173-174 (1984); Ovchinnikov et al.,
Molec.Gen.Genet.190:344-348 (1983); Severinov et al., J.Biol.Chem., 268:14820-
14825
(1993); Severinov et al., Molec.Gen.Genet., 244:120-126 (1994)].
25 Although, there was initial optimism in the middle of this century that
diseases
caused by bacteria would be quickly eradicated, it has become evident that the
so-called
"miracle drugs" are not sufficient to accomplish this task. Indeed, antibiotic
resistant
pathogenic strains of bacteria have become common-place, and bacterial
resistance to the
new variations of these drugs appears to be outpacing the ability of
scientists to develop
3 o effective chemical analogs of the existing drugs [See, Stuart B. Levy, The
Challenge of
Antibiotic Resistance, in Sciefatific American, 46-53 (March, 1998)].
Therefore, new
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CA 02440409 2003-09-09
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approaches to drug development are necessary to combat the ever-increasing
number of
antibiotic-resistant pathogens.
Classical penicillin-type antibiotics effect a single class of proteins known
as
autolysins. Thus, the development of new drugs which effect an alternative
bacterial target
protein would be desirable. Such a target protein ideally would be
indispensable fox
bacterial survival. A enzyme such as bacterial RNAP would thus be a prime
candidate for
such drug development.
Therefore, there is a need to develop methods for identifying drugs that
interfere
with bacterial RNAP. Unfortunately, such identification has heretofore relied
on
i o serendipity and/or systematic screening of large numbers of natural and
synthetic
compounds. One superior method for drug screening relies on structure based
rational drug
design. In such cases, a three dimensional structure of the protein or peptide
is determined
and potential agonists and/or antagonists are designed with the aid of
computer modeling
[Bugg et al., Scientific AnZericara, Dec.: 92-98 (1993); West et al., TIPS,
16:67-74 (1995);
i5 Dunbrack et al., Folding & Design, 2:27-42 (1997)].
Therefore, there is a need for obtaining a crystal of the bacterial RNAP bound
to an
inhibitor that is amenable to high resolution X-ray crystallographic analysis.
In addition,
there is a need for determining the three-dimensional structure of the RNAP
bound to that
inhibitor. Furthernlore, there is a need for developing procedures of
structure based rational
z o drug design using such three-dimensional information. Finally, there is a
need to employ
such procedures to develop new anti-bacterial drugs. The citation of any
reference herein
should not be construed as an admission that such reference is available as
"Prior Art" to the
instant application.
SUMMARY OF THE INVENTION
2 s The present invention provides crystals of RNA polymerase bound to an
inhibitor.
More particularly, the present invention provides crystals of the bacterial
core RNA
polymerase bound to rifampicin (the Rif RNAP complex). In addition, the
present
invention also provides detailed three-dimensional structural data for the Rif
RNAP
complex. The structural data obtained for the Rif RNAP complex can be used for
the
3 o rational design of drugs that inhibit bacterial cell proliferation. The
present invention
further provides methods of identifying and/or improving inhibitors of the
bacterial core
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RNA polymerase which can be used in place of and/or in conjunction with other
bacterial
inhibitors including antibiotics.
One aspect of the present invention provides crystals of the bacterial core
RNA
polymerase bound to rifampicin that can effectively diffract X-rays for the
determination of
s the atomic coordinates of the Rif RNAP complex to a resolution of better
than 5.0
Angstroms. In a preferred embodiment the crystal effectively diffracts X-rays
for the
determination of the atomic coordinates of the Rif RNAP complex to a
resolution of 3.5
Angstroms or better. In a particular embodiment the crystal of the Rif RNAP
complex
effectively diffracts X-rays for the determination of the atomic coordinates
to a resolution of
z o 3.3 Angstroms or better.
In a particular embodiment the bacterial core RNA polymerase of the crystal is
a
thermophilic bacterial core RNA polymerase. In a preferred embodiment of this
type the
thermophilic bacterial core RNA polymerase is a TheYnaus aquaticus (Taq)
bacterial core
RNA polymerase. Such a core RNA polymerase comprises a (3' subunit, a (3
subunit, and a
15 pair of a subunits. Preferably, the core RNA polymerase further comprises
an w subunit. In
a particular embodiment the (3' subunit has the amino acid sequence of SEQ ID
NO:1. In
another embodiment the [3 subunit has the amino acid sequence of SEQ 1D N0:2.
In still
another embodiment an a subunit has the amino acid sequence of SEQ ID N0:3. In
still
another embodiment an co subunit has the amino acid sequence of SEQ ID N0:4.
~ o In a preferred embodiment the core RNA polymerase is comprised of a (3'
subunit
having the amino acid sequence of SEQ ID NO: l, a (3 subunit having the amino
acid
sequence of SEQ ID N0:2, and a pair of a subunits having the amino acid
sequence of SEQ
ID N0:3. More preferably, this core RNA polymerase further comprises an ~
subunit
having the amino acid sequence of SEQ ID N0:4.
25 A crystal of the present invention may take a variety of forms all of which
are
included in the present invention. In a particular embodiment the crystal of
the RNA
polymerase has a space group of P41212 and a unit cell of dimensions of a=
b=201 and c=
294 A.
The present invention further includes methods of preparing a crystal of the
core
3 o RNA polymerase bound to an RNAP binding partner, e.g, an RNAP inhibitor
such as
rifampicin. A particular method comprises first growing a core bacterial RNA
polymerase
crystal in a buffered solution. One such buffered solution exemplified below,
contains 40-
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45% saturated ammonium sulfate. In one such embodiment the growing is
performed by
batch crystallization. In another embodiment the growing is performed by vapor
diffusion.
In yet another embodiment the growing is performed by microdialysis.
The crystals can be subsequently soaked in a stabilization solution, (e.g., 2
M
(NH4)ZSO4, 0.1 M Tris-HCI, pH 8.0, and 20 mM MgCl2) with an RNAP binding
partner
such as rifampicin (0.1 mM rifampicin was added in the Example below). The
RNAP/RNAP-binding partner are preferably incubated in the stabilization buffer
for at least
twelve hours. The crystals are then prepared for cryo-crystallography by
soaking the
RNAPIRNAP-binding partner complex in a stabilization buffer (e.g., 2 M
(NH4)ZS04, 0.1
io M Tris-HCI, pH 8.0, and 20 mM MgCl2 containing 50% (w/v) sucrose) before
flash
freezing. As exemplified below, crystals of the Rif RNAP complex were prepared
by
soaking the Rif RNAP complex for 30 minutes in stabilization buffer prior to
flash freezing
in liquid nitrogen.
Alternatively, the core RNA polyrnerase bound to an RNAP binding partner, e.g,
an
15 RNAP inhibitor such as rifampicin, can be co-crystallized under the
conditions as described
above.
Preferably the crystal of the Rif RNAP complex effectively diffracts X-rays
for the
determination of the atomic coordinates of the Rif RNAP complex to a
resolution of better
than 5.0 Angstroms. In a preferred embodiment the crystal effectively
diffracts X-rays for
a o the determination of the atomic coordinates of the Rif RNAP complex to a
resolution of 3.5
Angstroms or better. In a particular embodiment the crystal effectively
diffracts X-rays for
the determination of the atomic coordinates of the Rif RNAP complex to a
resolution of
3.3 Angstroms or better.
In a particular embodiment the crystal is grown by vapor diffusion. In one
such
a 5 embodiment the crystal is grown by hanging-drop vapor diffusion. In
another embodiment
the crystal is grown by sitting-drop vapor diffusion. Standard micro and/or
macro seeding
may be used to obtain a crystal of X-ray quality, i. e. a crystal that will
diffract to allow
resolution better than 5.0 Angstroms.
In addition, the present invention provides three-dimensional coordinates for
the
3 o Rif RNAP complex. In a particular embodiment the coordinates are for the
Rif RNAP
complex using the Tlr.ermus aquaticus core RNA polyrnerase as disclosed in
Table 2 (in
Appendix following the Sequence Listing). Thus the dataset of Table 2 below,
is part of the
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present invention. Furthermore, the dataset of Table 2 below, in a computer
readable form
is also part of the present invention.
The present invention also provides a molecule or molecular complex including
at
least a portion of an RNAP, e.g., a subunit, or RNAP substrate binding pocket.
In certain
s instances, the substrate binding pocket includes the amino acids listed in
Table 3, the
substrate binding pocket being defined by a set of points having a root mean
square
deviation of less than about 1.5~ from points representing the backbone atoms
of the amino
acids as represented by the structure coordinates listed in Table 2.
Preferably a substrate
binding pocket includes the amino acids listed in Table 3. More preferably a
substrate
i o binding pocket includes the amino acids listed in Table 4. The RNAP or
RNAP substrate
binding pocket can be alone or in a complex with a molecule, e.g., rifampicin.
In another aspect, the present invention provides a molecule or molecular
complex
that is structurally homologous to Taq RNAP molecule or molecular complex,
wherein the
RNAP molecule or molecular complex is represented by at least a portion of the
structure
z5 coordinates listed in Table 2.
In another aspect, the present invention provides a scalable three-dimensional
configuration of points. In one embodiment, at least a portion of the points
are derived from
structure coordinates of at least a portion of an RNAP molecule or molecular
complex listed
in Table 2 including at least one of an RNAP or RNAP substrate binding pocket.
a o Preferably, substantially all of the points are derived from structure
coordinates of an RNAP
molecule or molecular complex listed in Table 2. Preferably at least a portion
of the points
derived from the RNAP structure coordinates are derived from structure
coordinates
representing the locations of at least the backbone atoms of amino acids
defining an RNAP
substrate binding pocket, the substrate binding pocket including the amino
acids listed in
2 s Table 3. More preferably, the substrate binding pocket includes the amino
acids listed in
Table 4.
Advantageously, the scalable three-dimensional configuration of points may be
displayed as a holographic image, a stereodiagram, a model or a computer-
displayed image.
In another embodiment, at least a portion of the points of the scalable three
3 o dimensional configuration of points are derived from structure coordinates
of at least a
portion of a molecule or a molecular complex that is structurally homologous
to an RNAP
molecule or molecular complex and includes at least one of an RNAP or RNAP
substrate

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binding pocket. In certain instances, such configuration includes those amino
acids listed in
Table 3 or Table 4.
Still another aspect of the present invention comprises a method of using a
crystal of
the present invention and/or a dataset (including in computer readable form)
comprising
s the three-dimensional coordinates obtained from the crystal in a drug
screening assay.
In a particular embodiment of this type, the coordinates contained in the
dataset of
Table 2 below, can be used to identify potential modulators of the core RNA
polymerise.
In a preferred embodiment, the modulator is designed to interfere with the
bacterial RNAP,
but not to interfere with the human RNAP.
1 o Accordingly, the present invention provides methods of identifying an
agent or drug
that can be used to treat bacterial infections. One such embodiment comprises
a method of
identifying an agent for use as an inhibitor of bacterial RNA polymerise using
a crystal of a
Rif RNAP complex and/or a dataset comprising the three-dimensional coordinates
obtained
from the crystal. In a particular embodiment the three-dimensional coordinates
of the Rif
15 RNAP complex are determined using the Tlae~mus aquaticus core RNA
polymerise.
Preferably the crystal of the Rif RNAP complex effectively diffracts X-rays
for the
determination of the atomic coordinates to a resolution of, or better than 3.5
Angstroms.
More preferably the crystal of the Rif RNAP complex effectively diffracts X-
rays for the
determination of the atomic coordinates to a resolution of, or better than 3.3
Angstroms.
a o Preferably the selection is performed in conjunction with computer
modeling.
In one embodiment the potential agent is selected by performing rational drug
design with the three-dimensional coordinates determined for the crystal. As
noted above,
preferably the selection is performed in conjunction with computer modeling.
The potential
a
agent is then contacted with the bacterial RNA polymerise and the activity of
the bacterial
25 RNA polymerise is determined (e.g., measured). A potential agent is
identified as an agent
that inhibits bacterial RNA polymerise when there is a decrease in the
activity determined
for the bacterial RNA polymerise.
In a preferred embodiment the method further comprises preparing a
supplemental
crystal containing the core RNA polymerise bound to the potential agent.
Preferably the
3 o supplemental crystal effectively diffracts X-rays for the determination of
the atomic
coordinates to a resolution of better than 5.0 Angstroms, more preferably to a
resolution
equal to or better than 3.5 Angstroms, and even more preferably to a
resolution equal to or
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better than 3.3 Angstroms. The three-dimensional coordinates of the
supplemental crystal
are then determined with molecular replacement analysis and a second
generation agent is
selected by performing rational drug design with the three-dimensional
coordinates
determined for the supplemental crystal. Preferably the selection is performed
in
s conjunction with computer modeling.
As should be readily apparent the three-dimensional structure of a
supplemental
crystal can be determined by molecular replacement analysis or multiwavelength
anomalous
dispersion or multiple isomorphous replacement. A candidate drug is then
selected by
performing rational drug design with the three-dimensional structure
determined for the
~o supplemental crystal, preferably in conjunction with computer modeling. The
candidate
drug can then be tested in a large number of drug screening assays using
standard
biochemical methodology exemplified herein.
The method can further comprise contacting the second generation agent with a
eukaryotic RNA polymerase and determining (e.g., measuring) the activity of
the eukaryotic
15 RNA polymerase. A potential agent is then identified as an agent for use as
an inhibitor of
bacterial RNA polymerase when there is significantly less change (a factor of
two or more)
in the activity of the eukaryotic RNA polymerase relative to that observed for
the bacterial
RNA polymerase. Preferably no, or alternatively minimal change (i.e., less
than 15%) in
the activity of the eukaryotic RNA polymerase is determined.
a o The present invention further provides a method of identifying an agent
that inhibits
bacterial growth using the crl~fya Rif RNAP complex or a dataset comprising
the three-
dimensional coordinates obtained from the crystal. In a particular embodiment
the
three-dimensional coordinates of the Rif RNAP complex are determined with the
Therfnus
aquaticus core RNA polyrnerase.
25 Preferably the Rif RNAP complex effectively diffracts X-rays for the
determination
of the atomic coordinates to a resolution of, or better than 3.5 Angstroms.
More preferably
the Rif RNAP complex effectively diffracts X-rays for the determination of the
atomic
coordinates to a resolution of, or better than 3.3 Angstroms. Preferably the
selection is
performed in conjunction with computer modeling.
3 o In one embodiment the potential agent is selected by performing rational
drug
design with the three-dimensional coordinates determined for the crystal of
the Rif RNAP
complex. As noted above, preferably the selection is performed in conjunction
with
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computer modeling. The potential agent is contacted with and/or added to a
bacterial
culture and the growth of the bacterial culture is determined. A potential
agent is identified
as an agent that inhibits bacterial growth when there is a decrease in the
growth of the
bacterial culture. The method can further comprise preparing a supplemental
crystal
s containing the core RNA polymerase formed in the presence of the potential
agent.
Preferably the supplemental crystal effectively diffracts X-rays for the
determination of the
atomic coordinates to a resolution of better than 5.0 Angstroms, more
preferably to a
resolution equal to or better than 3.5 Angstroms, and even more preferably to
a resolution
equal to or better than 3.3 Angstroms. The three-dimensional coordinates of
the
1 o supplemental crystal are then determined with molecular replacement
analysis and a second
generation agent is selected by performing rational drug design with the three-
dimensional
coordinates determined for the supplemental crystal. Preferably the selection
is performed
in conjunction with computer modeling. The candidate drug can then be tested
in a large
number of drug screening assays using standard biochemical methodology
exemplified
15 herein.
In a particular embodiment the second generation agent is contacted with a
eukaryotic cell and the amount of proliferation of the eukaryotic cell is
determined. A
potential agent is identified as an agent for inhibiting bacterial growth when
there is
significantly less change (a factor of two or more) in the proliferation of
the eukaryotic cell
z o relative to that observed for the bacterial cell. Preferably no, or
alternatively minimal
change (i.e., less than 15%) in the proliferation of the eukaryotic cell is
determined.
. Computer analysis may be performed with one or more of the computer programs
including: QUANTA, CHARMM, INSIGHT, SYBYL, MACROMODEL and ICM
[Dunbrack et al., Foldiiag & Design, 2:27-42 (1997)]. In a further embodiment
of this
z5 aspect of the invention, an initial drug screening assay is performed using
the three-
dimensional structure so obtained, preferably along with a docking computer
program.
Such computer modeling can be performed with one or more Docking programs such
as
DOC, GRAM and AUTO DOCK [Dunbrack et al., Folding ~ Design, 2:27-42 (1997)].
In another aspect, the present invention provides a method for obtaining
structural
s o information about a molecule or a molecular complex of unknown structure
including
crystallizing the molecule or molecular complex; generating an x-ray
diffraction pattern
from the crystallized molecule or molecular complex; applying at least a
portion of the
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structure coordinates set forth in Table 2 to the x-ray diffraction pattern to
generate a three-
dimensional electron density map of at least a portion of the molecule or
molecular
complex whose structure is unknown.
In another aspect, the present invention provides a method for homology
modeling
s an RNAP homolog including: aligning-the amino acid sequence of an RNAP
homolog with
an amino acid sequence of RNAP (SEQ ID NO: 2) and incorporating the sequence
of the
RNAP homolog into a model of RNAP derived from structure coordinates set forth
in Table
2 to yield a preliminary model of the RNAP homolog; subjecting the preliminary
model to
energy minimization to yield an energy minimized model ; remodeling regions of
the
1 o energy minimized model where stereochemistry restraints are violated to
yield a final model
of the RNAP homolog.
In another aspect, the present invention provides a computer-assisted method
for
identifying an inhibitor of RNAP activity including: supplying a computer
modeling
application with a set of structure coordinates of a molecule or molecular
complex, the
15 molecule or molecular complex including at least a portion of an RNAP or
RNAP substrate
binding pocket, the substrate binding pocket including in certain instances
the amino acids
listed in Table 3; supplying the computer modeling application with a set of
structure
coordinates of a chemical entity; and determining whether the chemical entity
is an inhibitor
expected to bind to or interfere with the molecule or molecular complex,
wherein binding to
a o or interfering with the molecule or molecular complex is indicative of
potential inhibition
of RNAP activity. Preferably the substrate binding pocket includes the amino
acids listed in
Table 3, the substrate binding pocket being defined by a set of points having
a root mean
square deviation of less than about 1.5~ from points representing the atoms of
the amino
acids as represented by structure coordinates listed in Table 2.
Alternatively, the substrate
25 binding pocket includes the amino acids listed in Table 3, the substrate
binding pocket
being def ned by a set of points having a root mean square deviation of less
than about 1.5~
from points representing the backbone atoms of the amino acids as represented
by structure
coordinates listed in Table 2. Alternatively, the substrate binding pocket
includes the amino
acids listed in Table 3, the substrate binding pocket being defined by a set
of points having
3 o a root mean square deviation of less than about 1.5~ from points
representing the side chain
atoms of the amino acids as represented by structure coordinates listed in
Table 2.
Preferably determining whether the chemical entity is an inhibitor expected to
bind to or
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interfere with the molecule or molecular complex includes performing a fitting
operation
between the chemical entity and a binding pocket of the molecule or molecular
complex,
followed by computationally analyzing the results of the fitting operation to
quantify the
association between the chemical entity and the binding pocket. Preferably the
method
s further includes screening a library of chemical entities. Preferably the
method further
includes supplying or synthesizing the potential inhibitor, then assaying the
potential
inhibitor to determine whether it inhibits RNAP activity.
In another aspect, the present invention provides a computer-assisted method
for
designing an inhibitor of RNAP activity including: supplying a computer
modeling
i o application with a set of structure coordinates of a molecule or molecular
complex, the
molecule or molecular complex including at least a portion of an RNAP or RNAP
substrate
binding pocket, the substrate binding pocket including the amino acids listed
in Table 3;
supplying the computer modeling application with a set of structure
coordinates for a
chemical entity; evaluating the potential binding interactions between the
chemical entity
i5 and substrate binding pocket of the molecule or molecular complex;
structurally modifying
the chemical entity to yield a set of structure coordinates for a modified
chemical entity ;
and determining whether the modified chemical entity is an inhibitor expected
to bind to or
interfere with the molecule or molecular complex, wherein binding to or
interfering with
the molecule or molecular complex is indicative of potential inhibition of
RNAP activity.
z o Preferably the substrate binding pocket includes the amino acids listed in
Table 3, the
substrate binding pocket being defined by a set of points having a root mean
square
deviation of less than about 1.5~ from points representing the backbone atoms
of the amino
acids as represented by structure coordinates listed in Table 2. Preferably
determining
whether the modified chemical entity is an inhibitor expected to bind to or
interfere with the
z s molecule or molecular complex includes performing a fitting operation
between the
chemical entity and a binding pocket of the molecule or molecular complex,
followed by
computationally analyzing the results of the fitting operation to quantify the
association
between the chemical entity and the binding pocket. Preferably the set of
structure
coordinates for the chemical entity is obtained from a chemical fragment
library. Preferably
3 o the method fizrther includes supplying or synthesizing the potential
inhibitor, then assaying
the potential inhibitor to determine whether it inhibits RNAP activity.
In another aspect, the present invention provides a computer-assisted method
for
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designing an inhibitor of RNAP activity de novo including: supplying a
computer modeling
application with a set of structure coordinates of a molecule or molecular
complex, the
molecule or molecular complex including at least a portion of an RNAP or RNAP
substrate
binding pocket, wherein the substrate substrate binding pocket includes the
amino acids
s listed in Table 3 ; computationally building a chemical entity represented
by set of structure
coordinates ; and determining whether the chemical entity is an inhibitor
expected to bind
to or interfere with the molecule or molecular complex, wherein binding to or
interfering
with the molecule or molecular complex is indicative of potential inhibition
of RNAP
activity. Preferably the substrate binding pocket includes the amino acids
listed in Table 3,
i o the substrate binding pocket being defined by a set of points having a
root mean square
deviation of less than about 1.51 from points representing the backbone atoms
of the amino
acids as represented by structure coordinates listed in Table 2. Preferably
determining
whether the chemical entity is an inhibitor expected to bind to or interfere
with the molecule
or molecular complex includes performing a fitting operation between the
chemical entity
i5 and a binding pocket of the molecule or molecular complex, followed by
computationally
analyzing the results of the fitting operation to quantify the association
between the
chemical entity and the binding pocket. Preferably the method further includes
supplying or
synthesizing the potential inhibitor, then assaying the potential inhibitor to
determine
whether it inhibits RNAP activity.
~ o In another aspect, the present invention provides a method for making an
inhibitor
of RNAP activity, the method including chemically or enzymatically
synthesizing a
chemical entity to yield an inhibitor of RNAP activity, the chemical entity
having been
identified during a computer-assisted process including supplying a computer
modeling
application with a set of structure coordinates of a molecule or molecular
complex, the
2 s molecule or molecular complex including at least a portion of at least one
of a RNAP or
RNAP substrate binding pocket ; supplying the computer modeling application
with a set of
structure coordinates of a chemical entity; and determining whether the
chemical entity is
expected to bind to or interfere with the molecule or molecular complex at a
binding
pocket, wherein binding to or interfering with the molecule or molecular
complex is
3 o indicative of potential inhibition of RNAP activity.
In another aspect, the present invention provides a method for making an
inhibitor
of RNAP activity, the method including chemically or enzymatically
synthesizing a
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chemical entity to yield an inhibitor of RNAP activity, the chemical entity
having been
designed during a computer-assisted process including supplying a computer
modeling
application with a set of structure coordinates of a molecule or molecular
complex, the
molecule or molecular complex including at least a portion of at least one of
a RNAP or
s RNAP substrate binding pocket ; supplying the computer modeling application
with a set of
structure coordinates for a chemical entity ; evaluating the potential binding
interactions
between the chemical entity and a binding pocket of the molecule or molecular
complex ;
structurally modifying the chemical entity to yield a set of structure
coordinates for a
modified chemical entity ; and determining whether the chemical entity is
expected to bind
1 o to or interfere with the molecule or molecular complex at the binding
pocket, wherein
binding to or interfering with the molecule or molecular complex is indicative
of potential
inhibition of RNAP activity.
In another aspect, the present invention provides a method for making an
inhibitor
of RNAP activity, the method including chemically or enzymatically
synthesizing a
is chemical entity to yield an inhibitor of RNAP activity, the chemical entity
having been
designed during a computer-assisted process including supplying a computer
modeling
application with a set of structure coordinates of a molecule or molecular
complex, the
molecule or molecular complex including at least a portion of at least one of
a RNAP or
RNAP substrate binding pocket ; computationally building a chemical entity
represented by
a o set of structure coordinates ; and determining whether the chemical entity
is expected to
bind to or interfere with the molecule or molecular complex at a binding
pocket, wherein
binding to or interfering with the molecule or molecular complex is indicative
of potential
inhibition of RNAP activity.
It should be understood that in all of the drug screening assays provided
herein, a
a s number of iterative cycles of any or all of the steps may be performed to
optimize the
selection. For example, assays and drug screens that monitor the activity of
the RNA
polymerase in the presence and/or absence of a potential modulator (or
potential drug) are
also included in the present invention and can be employed as the sole assay
or drug screen,
or more preferably as a single step in a mufti-step protocol for identifying
modulators of
3 o bacterial proliferation and the like.
The present invention further provides the novel agents (modulators or drugs)
that
are identified by a method of the present invention, along with the method of
using agents
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(modulators or drugs) identified by a method of the present invention, for
inhibiting
bacterial RNA polymerise and/or bacterial proliferation.
The present invention further provides an apparatus that comprises a
representation
of a Rif RNAP complex. One such apparatus is a computer that comprises the
s representation of the Rif RNAP complex in computer memory. In one
embodiment, the
computer comprises a machine-readable data storage medium which contains data
storage
material that is encoded with machine-readable data which comprises the atomic
coordinates obtained from a crystal of the Rif RNAP complex. Preferably the
computer
comprises a machine-readable data storage medium which contains data storage
material
s o that is encoded with machine-readable data which comprises the structural
coordinates of
Table 2. In one embodiment, the computer comprises a machine-readable data
storage
medium which contains data storage material that is encoded with machine-
readable data
which comprises the structural coordinates obtained from a crystal of the Rif
RNAP
complex. More preferably the computer further comprises a working memory for
storing
is instructions for processing the machine-readable data, a central processing
unit coupled to
both the working memory and to the machine-readable data storage medium for
processing
the machine readable data into a three-dimensional representation of the Rif
RNAP
complex. In a preferred embodiment, the computer also comprises a display that
is coupled
to the central-processing unit for displaying the three-dimensional
representation.
a o In another aspect, the present invention provides a machine-readable data
storage
medium including a data storage material encoded with a first set of machine
readable data
which, when combined with a second set of machine readable data, using a
machine
programmed with instructions for using the first set of data and the second
set of data, can
determine at least a portion of the structure coordinates corresponding to the
second set of
z s machine readable data, wherein the first set of data includes a Fourier
transform of at least a
portion of the structure coordinates for RNAP listed in Table 2 ; and the
second set of data
includes an x-ray diffraction pattern of a molecule or molecular complex of
unknown
structure.
Accordingly, it is a principal object of the present invention to provide a
crystal
3 o containing the Rif RNAP complex.
It is a further object of the present invention to provide the three-
dimensional
coordinates of the Rif RNAP complex for the T7aeYmus aquaticus core RNA
polymerise.
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It is a further object of the present invention to provide methods for the
rational
design of drugs that inhibit prokaryotic RNA polymerise.
It is a further object of the present invention to provide methods of
identifying drugs
that can modulate bacterial proliferation.
It is a further object of the present invention to provide methods for the
rational
design of drugs that inhibit bacterial proliferation without negatively
effecting human RNA
polymerise.
It is a further object of the present invention to provide methods of
identifying
agents that can be used to treat bacterial infections in mammals, and
preferably in humans.
s o These and other aspects of the present invention will be better
appreciated by
reference to the following drawings and Detailed Description.
BRIEF DESCRIPTION OF THE DRAWINGS
r
m Figure 1 depicts the rifampicin {Rif) resistant regions of the RNAP (3
subunit. The
bar on top schematically represents the E. coli [3 subunit primary sequence
with amino acid
numbering shown directly above. Gray boxes within the schematic indicate
evolutionarily
conserved regions among all prokaryotic, chloroplast, archaebacterial, and
eukaryotic
sequences labeled A-I at the top [Allison et al., Cell 42:599-610 (1985);
Sweetser et al.,
a o Proc.Natl.Acad.sci. USA 84:1192-1196 (1987)]. Red markings indicate the
four clusters
where Rifa mutations have been identified in E. coli [Jin and Gross,
J.Molec.Biol,
202:45-58 (1988); Lisitsyn et al., BioorgKhirn 10:127-128 (1984); Lisitsyn et
al.,
Molec.Gen.Genet., 196:173-174 (1984); Ovchinnikov et al.,
Molec.Gera.Gen.et.190:344-348
(1983); Severinov et al., J.Biol.Chem., 268:14820-14825 (1993); Severinov et
al.,
25 Molec.Gen.Genet., 244:120-126 (1994)] denoted as the N-terminal cluster
(N), and clusters
I, II and III (I, II, III). Directly below is a sequence alignment spanning
these regions of the
E. coli (E.c.), T, aquaticus (T.a.), and M. tuberculosis (M. t) RNAP (3
subunits. Amino
acids that are identical to E. coli are shaded dark gray, and those that are
homologous (ST,
RK, DE, NQ, FYWIV) are shaded light gray. Mutations that confer Rife in E.
coli and M.
s o tuberculosis are indicated directly above (for E. coli) or below (for M.
tuberculosis) as
follows: 0 for deletions, S2 for insertions, and colored dots for amino acid
substitutions
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CA 02440409 2003-09-09
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(substitutions at each position are indicated in single-amino acid code in
columns above or
below the positions).
Color-coding for the amino acid substitutions (for reference to subsequent
figures):
(i) empty dots, residues that interact directly with the bound rifampicin (see
Fig.
s 4a-4b);
(ii) full dots, residues that are too far away from the rifampicin for direct
interaction (see Fig. 5a-Sb); and
(iii) dots with a line through, three positions that are substituted with high
frequency (noted as a % immediately below the substitutions) in clinical
isolates of Rid M.
1o tuberculosis [Ramaswamy and Musser, Tubercle and Lung Disease 79:3-29
(1998)].
Below the three prokaryotic sequences is a sequence alignment of three
eukaryotic
sequences with shading as above. The dots indicate a gap in the alignment.
Figures 2a-2d show that the rifampicin inhibition of Taq RNAP. Figure 2a
depicts
autoradiographs showing the radioactive RNA produced by Taq (lanes 1-7) and E.
coli
15 (lanes 8-13) RNAP holoenzymes transcribing a template containing the T7 Al
promoter
and the tR2 terminator, analyzed on a 15% polyacrylamide gel and quantitated
by
phosphorimagery. In the absence of rifampicin (lanes 1 and 8), the major RNA
products
from each RNAP correspond to a trimeric abortive product (CpApU), a 105
nucleotide
terminated transcript (Term), and a 127 nucleotide runoff transcript (Run
offj. Lanes 2-7
a o and 9-13 show the effects of increasing concentrations of rifampicin.
Figure 2b shows the
quantitated results, where the amounts of each product (normalized to 100% for
the Run off
and Term transcripts in the absence of rifampicin, and for CpApU at the
highest
concentration of rifampicin) are plotted as a function of rifampicin
concentration. Figure 2c
shows the distance between the bound rifampicin and the initiating substrate
(i-site) of E.
z s coli and Taq RNAP holoenzymes measured using chimeric Rif nucleotide
compounds as
previously described [Mustaev et al., Proc.Nat.Acad.Sci. USA 91:12036-12040
(1994)].
Rif nucleotide compounds (Rif (CH2)n-Ap) with different linker lengths, n
(indicated
above each lane) were bound to RNAP, then extended in a specific transcription
reaction
with a-[3zP]UTP by the RNAP catalytic activity. The products were analyzed on
a 23%
3 o polyacrylamide gel, visualized by autoradiography, and quantitated by
phosphorimagery.
Figure 2d shows the quantitated results where the product yield (as % activity
normalized to
100% at the highest level) is plotted as a function of the Rif nucleotide
linker length (n).
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Figures 3a-3c show the Rif RNAP co-crystal structure. Figure 3a is a
stereoview of
the Rif binding pocket of Taq core RNAP, generated using O [Jones et al., Acta
Cryst, A
47:110-119 (1991)]. Carbon atoms of the RNAP (3 subunit are cyan or yellow
(residues
within 4 ~ of the rifampicin), while carbon atoms of the inhibitor are orange.
Oxygen
s atoms are red, nitrogen atoms are blue, and sulfur atoms are green. Electron
density,
calculated using (~Fo 'f - Fonacl) coefficients is shown (orange) for the Rif
only (contoured at
3.5 a), and was computed using phases from the final refined RNAP model with
the
rifampicin omitted [see Zhang et al., Cell 98:811-824 (1999) and U.S. Patent
No.
6,225,076, which is incorporated by reference herein]. Here, "Rif' denotes the
Rif RNAP
i o co-crystal, and "native" denotes the native core RNAP crystal. Figure 3b
shows the
three-dimensional structure of Taq core RNAP in complex with rifampicin
generated using
GRASP [Nicholls et al., Proteiyas Structure, Furactioh and Geraetics 11:281-
296 (199I)].
The backbone of the RNAP structure is shown as tubes, along with the color-
coded
transparent molecular surface ((3, cyan; (f, pink; w, white; the a-subunits
are behind the
i5 RNAP and are not visible). The Mg2+ ion chelated at the active site is
shown as a magenta
sphere. The rifampicin is shown as CPK atoms (carbon, orange; oxygen, red;
nitrogen,
blue). Figure 3c is the structural formula of rifampicin. Features of the
structure discussed
in the text are color-coded (ansa bridge, blue; napthol ring, green). The four
oxygen atoms
critical for rifampicin activity [Arora, Acta Crystall. B37:152-157 (1981);
Arora, Molecular
2o Pharmacology 23:133-140 (1983); Arora, J.Med.Chem. 28:1099-1102 (1985);
Arora and
Main, .l. A~atibiot. 37:178-181 (1984); Brufani et al., J. Molec.Biol. 87:409-
435 (1974);
Lancini and Zanichelli, In Structure-activity Relationslaip ira
Semisyratlaetic Afatibiotics, D.
Perlaman, ed. (Academic Press), pp. 53I-600 (1977); Sensi et al.,
Rev.Infect.I~is., 5
Supp.3:402-406 (1983)] are shaded with red circles.
2 s Figures 4a-4b depict the detailed interactions of rifampicin with RNAP.
Figure 4a
is a stereoview of the Taq RNAP Rif binding pocket complexed with rifampicin,
generated
using RIBBONS [Carson, J.Appl.Crystall., 24:958-961 (1991)], showing residues
that
interact directly with the inhibitor. The backbone of the (3 subunit is shown
as a cyan
ribbon. Side chains (and backbone atoms of F394) of residues within 4 ~ of
rifampicin are
3 o shown. Carbon atoms are orange (Rif), magenta (three residues substituted
in M.
tuberculosis Rii~ clinical isolates with high frequency, see Fig.l), or
yellow; oxygen atoms
are red; nitrogen atoms are blue. The view is from above the ~3 subunit,
looking through [3
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CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
to the rifampicin, but with obscuring parts of [i removed. Potential hydrogen
bonds
between protein atoms and rifampicin are shown as dashed lines. Figure 4b
shows a
schematic drawing of RNAP [3 subunit interactions with rifampicin, modified
from
LIGPLOT [Wallace et al., Protein Engiraeeri~ag 8:127-134 (1995)]. Residues
forming
s van-der-Waals interactions are indicated: those participating in hydrogen
bonds are shown
in a ball-and-stick representation, with hydrogen bonds depicted as dashed
lines, carbon
atoms of the protein are black, while carbon atoms of rifampicin are orange.
Oxygen atoms
are red and nitrogen atoms are blue.
Figures Sa-Sb show the rifampicin binding pocket and Rif mutants as
stereoviews
l o of the Taq RNAP Rif binding pocket complexed with rifampicin. The view is
the same in
Fig.Sa and Sb and is rotated approximately 180° about the horizontal
axis from the view of
Fig. 4a. This view is from the middle of the main RNAP channel, looking
towards the
rifampicin, with the (3 subunit behind. Figure Sa shows the backbone of the [3
subunit as a
cyan ribbon, but with a highly conserved segment of region D (443-451, see
text) colored
15 red. Side chains (and backbone atoms of F394) of residues where
substitutions confer Rif
(see Fig. 1) are shown. Carbon atoms are orange (Rif), magenta (three residues
substituted
in M. tuberculosis Rif clinical isolates with high frequency, see Fig. 1 ),
yellow (other
residues that interact directly with rifampicin, as in Fig. 4), or green (all
other Rife
positions). Oxygen atoms are colored red; nitrogen atoms are blue. The
depiction was
a o generated using RIBBONS [Carson, J.Appl.Cystall., 24:958-961 (1991)]. The
(3 subunit is
shown in Figure Sb as a cyan molecular surface, with a highly conserved
segment of region
D colored red, and surface exposed Rif positions colored yellow (within 4 t~
of the Rif) or
green. The depiction was generated using GRASP [Nicholls et al., Proteins
Structure,
Futzctioft a~zd Genetics 11:281-296 (1991)].
25 Figures 6a and 6b show the mechanism of RNAP inhibition by rifampicin. The
RNAP active site Mgz+ (magenta sphere) and the 9-basepair RNA/DNA hybrid (from
+1 to
-8) from a model of the ternary elongation complex [Korzheva et al., Science
289:619-625
(2000)] are shown in Figure 6a. The RNAP itself and the rest of the nucleic
acids are
omitted for clarity. The incoming nucleotide substrate at the +1 position is
colored green,
3 o the -1 and -2 positions, which can be accommodated in the presence of
rifampicin, are
colored yellow. The RNA further upstream (-3 to -8), which cannot be
accommodated in
the presence of rifampicin is colored pink. The template strand of the DNA is
colored grey.
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Also shown is a CPK representation of rifampicin as it would be positioned in
its binding
site on the (3 subunit (carbon atoms, orange; oxygen, red; nitrogen, blue).
The rifampicin is
partially transparent, illustrating the RNA nucleotides at -3 to -5 that
sterically clash. This
depiction was generated using GRASP [Nicholls et al., Proteiyas Structure,
Fuyzctio~c and
s Genetics 11:281-296 (1991)]. The structure of the minimal scaffold systems
with RNA
lengths from 3-7 nucleotides (labeled above the RNA chain) are shown in Figure
6b
[Korzheva et al., Science 259:619-625 (2000)]. The results are presented below
as
autoradiographs of the radioactive RNAs produced by E. coli (lanes 1-15) or
Taq (lanes
16-30) core RNAPs transcribing the minimal scaffolds with the indicated
lengths of RNA
s o ('X =') and analyzed on a 23% polyacrylamide gel. Lanes 1-10 and 16-25
demonstrate the
effect of rifampicin inhibition on transcription when it was bound by RNAP
either before
(lanes 1-5 and 16-20) or after (lanes 6-10 and lanes 21-25) addition of the
scaffold. Lanes
11-1 S and 26-30 show elongation of the same scaffolds in the absence of
rifampicin. The
RNA with the critical length of 3 nucleotides which cannot be elongated by E.
coli RNAP in
15 the presence of rifampicin regardless of the order of rifampicin and
scaffold addition (lanes
1,6) is colored red. The RNAs of 4-7 nucleotides (colored green) were extended
by E. coli
RNAP when added before rifampicin (lanes 6-10).
Figure 7 depicts a schematic of a computer comprising a central processing
unit
("CPU"), a working memory, a mass storage memory, a display terminal, and a
keyboard
z o that are interconnected by a conventional bidirectional system bus. The
computer can be
used to display and manipulate the structural data of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides crystals of a bacterial core RNA polyrnerase
bound
to an inhibitor. The present invention further provides the structural
coordinates for a
z s bacterial core RNA polymerise bound to rifampicin (Rif RNAP complex) and
methods of
using such structural coordinates in drug assays. More particularly, the
present invention
provides the structural coordinates for the Rif RNAP complex with the
Tlz.ermus aquaticus
core RNA polymerise (see Table 2 in Appendix following the Sequence Listing).
Rifampicin (Rif) is one of the most potent and broad-spectrum antibiotics
against
3 o bacterial pathogens and is a key component of anti-tuberculosis therapy,
stemming from its
inhibition of the bacterial RNA polymerise (RNAP). The X-ray crystal structure
of
TheYmus aquaticus core RNA polymerise reveals a "crab-claw" shaped molecule
with a 27
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CA 02440409 2003-09-09
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1~ wide internal channel [see Zhang et al., Cell 98:811-824 (1999)]. As
disclosed herein,
rifampicin binds in a pocket of the RNAP [i subunit deep within the DNA/RNA
channel,
but more than 12 A away from the active site the crystal structure of Thermus
aquaticus
core RNAP complexed with rifampicin. The structure, combined with biochemical
results
disclosed herein, explains the effects of rifampicin on RNAP function and
indicates that the
inhibitor acts by directly blocking the path of the elongating RNA when the
transcript
becomes 2 to 3 nucleotides in length.
The three-dimensional structure disclosed herein demonstrates that rifampicin
binds
the Taq core RNAP with a close complementary fit in a pocket between two
structural
~ o domains of the RNAP (3 subunit. Only small, local conformational changes
of both the
inhibitor and the protein were observed. The binding site is deep within the
main RNAP
channel, but the closest approach of the inhibitor to the RNAP active site
Mg2+ is more than
12 A (Fig. 3b, below). The Rif binding pocket is surrounded by the 23 known
positions
where amino acid substitutions confer Rife (Fig. 5, below, and Table 4).
Twelve of these
1s residues are close enough to interact directly with the rifampicin (Figs.
4a-4b, below, and
Table 3). Predominant are van-der-Waals interactions with hydrophobic side-
chains near
the napthol ring of rifampicin, and potential hydrogen bond interactions with
5 polar groups
of rifampicin (2 on the napthol ring, and 3 on the ansa bridge), 4 of which
have been shown
to be essential for rifampicin activity. The remaining known Rif mutants are
one layer
a o removed from the rifampicin itself, and are likely to affect rifampicin
binding through small
structural distortions of the Rif binding pocket.
Therefore the structure disclosed herein explains the effects of rifampicin on
RNAP
fiznction determined from detailed biochemical and kinetic studies. In
combination with a
model of the ternary transcription complex, the structure indicates that the
predominant
as effect of rifampicin is to directly block the path of the elongating RNA
transcript at the
5'-end when the transcript becomes either 2 or 3 nucleotides in length,
depending on the
5'-phosphorylation state of the 5'-nucleotide (Figs. 6a-6b, below). In this
view, rifampicin
binds the Rif binding site of the RNAP holoenzyme either before or after the
binding of the
DNA template and formation of the open complex. Indeed, the binding of the DNA
s o template and the formation of the open complex are not affected by the
presence of
rifampicin. However, rifampicin has its effect after the nucleotide substrates
binds their
sites in the RNAP active site. Thus the initiating nucleotide substrate binds
the RNAP i-site
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CA 02440409 2003-09-09
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with a small, approximately 2-fold increase in the apparent Km due to the
presence of
rifampicin, while the second nucleotide binds in the i+1 site with little
notice of the
rifampicin. More or less normally, the RNAP then catalyzes the formation of a
phosphodiester bond between the two nucleotides. If the initiating nucleoside
bears a
5'-triphosphate, the subsequent translocation of the RNAP attempts to move the
2-nucleotide RNA transcript upstream such that the i+1 nucleotide occupies the
i-site (-1
position), and the i-site nucleotide moves into the -2 position (Fig. 6a,
below). The
movement of the 5'-nucleotide into the -2 position, however, results in a
severe steric clash
with the rifampicin. The molecular details of the ensuing events are unclear,
but in the end
1 o the RNAP remains at the same template position, the 2-nucleotide
transcript is released, and
the futile cycle begins again. If the 5'-nucleoside contains a di- or a mono-
phosphate at its
5'-end (or if it's unphosphorylated), then after the synthesis of the first
phosphodiester bond,
the RNAP can translocate normally and the steric clash of the transcript with
the bound
rifampicin occurs during the translocation of the 3-nucleotide transcript
following the
synthesis of the second phosphodiester bond.
The present invention exploits the structural information described herein,
including
the structural coordinates disclosed in Table 2, and provides methods of
identifying agents
or drugs that can be used to control the proliferation of bacteria, e.g., for
use as treatments
for bacterial infections.
a o Therefore, if appearing herein, the following terms shall have the
definitions set out
below:
As used herein the term "core RNA polymerase" minimally comprises the subunit
composition of a2(3(3' which is evolutionarily conserved from bacteria to man.
Preferably
the core RNA polymerase further comprises the co subunit. The three-
dimensional structure
2 s of the The~mus aquaticus core RNA polymerase is described in Zhang et al.,
Cell
98:811-824 (1999).
As used herein the term "RNAP" means a DNA-dependent RNA polymerase. In
certain instances, a RNAP is bacterial in origin or eukaryotic in origin.
Examples of
various RNAPs are described herein. As used herein "Rif RNAP" is used
interchangeably
3 o with the "Rif RNAP complex" and comprises the binding complex of
rifampicin with the
core RNA polymerase as disclosed in the Example below. The structural
coordinates for a
crystal of Rif RNAP are listed in Table 2 (in Appendix following the Sequence
Listing).
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CA 02440409 2003-09-09
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As used herein an "RNAP binding partner" is a small organic molecule or other
modulator that binds to RNAP. Preferably the RNAP binding partner is an
inhibitor of the
catalytic and/or the transcriptional activity of RNAP. Rifampicin is a small
organic
molecule that is a particular binding partner of RNAP that is exemplified
below.
"Molecular complex including at least a portion of an RNAP" refers to a
complex
comprising at least one subunit or portion of a RNAP.
"Taq RNAP molecule or molecular complex" refers to at least a portion of a
subunit
of Taq RNAP.
The term "binding pocket," as used herein, refers to a region of a molecule or
so molecular complex, that, as a result of its shape, favorably associates
with another chemical
entity or modulator. Exemplary binding pockets include active sites, surface
grooves or
contours or surfaces of a RNAP which are capable of participating in
interactions with
another modulator.
"RNAP substrate binding site" is used interchangeably herein with "RNAP
substrate
is binding pocket", "RNAP binding pocket" and other like terms and refers to a
region of a
RNAP molecule or molecular complex, that, as a result of its shape, favorably
associates
with another chemical entity or modulator. Exemplary binding pockets include
active sites,
surface grooves or contours or surfaces of a RNAP which are capable of
participating in
interactions with a modulator. The region to which a modulator can bind
include regions to
a o which a drug, such as rifampicin, can bind; regions to which a nucleic
acid, such as DNA,
can bind; regions to which a nucleotide can be bind; regions to which a
cation, such as
Mg2+, can bind; regions to which proteins bind; regions to which cofactors may
bind;
regions to which other entities bind to form a molecular complex containing
RNAP. One
example of such a site for a RNAP is the site defined by at least some of the
amino acids in
z 5 Table 3 or 4, as described in further detail herein, and is referred to
herein as "Rif RNAP
binding pocket."
"RNAP-like substrate binding pocket" refers to the substrate binding pocket of
an
RNAP-like molecule or complex.
A "homologue of Taq RNAP" includes molecules that are sufficiently related to
Taq
3 o RNAP, as further described herein. Exemplary homologues include
vertebrate, such as
mammalian, e.g., human, mouse, bovine, ovine, porcine, equine, canine, and
feline RNAP;
yeast RNAP; bacterial RNAP, such as E. coli RNAP; and other prokaryotic RNAPs.
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As used herein, the "transcriptional activity of RNAP" includes the ability of
RNAP
to carry out the elongation of the RNA transcript during transcription. Thus,
whereas the
catalytic activity of RNAP includes the binding of the enzyme to the
nucleotide substrates
and the subsequent formation of the phosphodiester bond between the two
substrates, the
s transcriptional activity includes the RNAP dependent elongation of the RNA
transcript at
the 5'-end.
As used herein an "active RNA polymerase" is an RNA polymerase that minimally
contains a pair of a subunits, a (i' subunit, and a (3 subunit; or fragments
thereof, but still
retains at least 25% of the catalytic and/or transcriptional activity of the
coxe RNA
1 o polymerase made up of the full length a, [3', and [3 subunits. Thus active
RNA polymerases
can comprise fragments of the a subunit and/or (3' subunit and/or (i subunit.
The term "modulation", when used in reference to a functional property or
biological
activity or process (e.g., enzyme activity or receptor binding), refers to the
capacity to either up
regulate (e.g., activate or stimulate) or down regulate (e.g., inhibit or
suppress) such property,
i s activity or process. In certain instances, such regulation may be
contingent on the occurrence
of a specific event, such as activation of a signal transduction pathway,
and/or may be manifest
only in particular cell types.
The term "motif' refers to an amino acid sequence that is commonly found in a
protein
of a particular structure or function. Typically a consensus sequence is
defined to represent a
2 o particular motif. The consensus sequence need not be strictly defined and
may contain
positions of variability, degeneracy, variability of length, etc. The
consensus sequence may be
used to search a database to identify other proteins that may have a similar
structure or function
due to the presence of the motif in its amino acid sequence. For example, on-
line databases
may be searched with a consensus sequence in order to identify other proteins
containing a
2 5 particular motif. Various search algorithms and/or programs may be used,
including FASTA,
BLAST or ENTREZ.
The term "structural motif' refers to a structural motif of a polypeptide or
protein that,
although it may have different amino acid sequences, may result in a similar
structure, wherein
by structure is meant that the motif forms generally the same tertiary
structure, or that certain
3 o amino acid residues within the motif, or alternatively their backbone or
side chains (which may
or may not include the Ca) are positioned in a like relationship with respect
to one another in
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the motif. Such structural motifs are known to be important to the
functionality observed for
proteins.
As used herein a "small organic molecule" is an organic compound [or organic
compound complexed with an inorganic compound (e.g., metal)] that has a
molecular
s weight of less than 3 I~d.
As used herein the term "about" means within 10 to 15%, preferably within 5 to
10%. For example an amino acid sequence that contains about 60 amino acid
residues can
contain between 51 to 69 amino acid residues, more preferably 57 to 63 amino
acid
residues.
i o As used herein a polypeptide or peptide "consisting essentially of or that
"consists
essentially of a specified amino acid sequence is a polypeptide or peptide
that retains the
general characteristics, e.g., activity of the polypeptide or peptide having
the specified
amino acid sequence and is otherwise identical to that protein in amino acid
sequence
except it consists of plus or minus 10% or fewer, preferably plus or rninus 5%
or fewer, and
is more preferably plus or minus 2.5% or fewer amino acid residues.
As used herein, and unless otherwise specified, the terms "agent", "potential
drug",
"test compound", "modulator" or "potential compound" are used interchangeably,
and refer
to chemicals which potentially have a use as a modulator (and preferably as an
inhibitor) of
bacterial RNA polymerase. More preferably, an agent is a drug that can be used
to treat
a o and/or prevent bacterial infection. Therefore, such "agents", "potential
drugs", and
"potential compounds" may be used, as described herein, in drug assays and
drug screens
and the like. An agent, for example, may be a polypeptide, nucleic acid,
macromolecule,
complex, molecule, organic small molecule, species or the like (naturally
occurring or non-
naturally occurring), or an extract made from biological materials such as
bacteria, plants,
2 s fungi, or animal cells or tissues, that may be capable of causing
modulation. Agents may be
evaluated for potential activity as inhibitors or activators (directly or
indirectly) of a
functional property, biological activity or process, or combination of them,
(e.g., agonist,
partial antagonist, partial agonist, inverse agonist, antagonist, anti-
microbial agents,
inhibitors of microbial infection or proliferation, and the like) by inclusion
in assays. In
3 o such assays, many agents may be screened at one time. The activity of any
agent may be
known, unknown or partially known.
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The term "test modulator" refers to a molecule to be tested by one or more
screening
methods) as a putative modulator of a RNAP. A test modulator is an example of
an agent or
modulator. A test modulator is usually not known to bind to a RNAP before
assaying. The
term "control test modulator" refers to a molecule known to bind to the target
(e.g., a known
agonist, antagonist, partial agonist or inverse agonist), here a RNAP. The
term "test
modulator" does not include a chemical added as a control condition that
alters the function of
the target to determine signal specificity in an assay. Such control chemicals
or conditions
include chemicals that 1) nonspecifically or substantially disrupt protein
structure (e.g.,
denaturing agents (e.g., urea or guandium), chaotropic agents, sulfhydryl
reagents (e.g.,
a. o dithiothritol and (3-mercaptoethanol), and proteases), 2) generally
inhibit cell metabolism (e.g.,
mitochondrial uncouplers) and 3) non-specifically disrupt electrostatic or
hydrophobic
interactions of a protein (e.g., high salt concentrations, or detergents at
concentrations sufficient
to non-specifically disrupt hydrophobic interactions). Further, the term "test
modulator" also
does not include molecules known to be unsuitable for a therapeutic use for a
particular
indication due to toxicity of the subject. In certain embodiments, various
predetermined
concentrations of test modulators are used for screening such as 0.01 ~,M, 0.1
~,M,1.0 pM, and
10.0 pM. Examples of test modulators include, but are not limited to, any
agent described
above. The term "novel test modulator" refers to a test modulator that is not
in existence as of
the filing date of this application. In certain assays using novel test
modulators, the novel test
a o modulators comprise at least about 50%, 75%, 85%, 90%, 95% or more of the
test modulators
used in the assay or in any particular trial of the assay.
The term "naturally-occurring", as applied to an object, refers to the fact
that an object
may be found in nature. For example, a polypeptide or polynucleotide sequence
that is present
in an organism (including bacteria) that may be isolated from a source in
nature and which has
z5 not been intentionally modified by man in the laboratory is naturally-
occurring.
Nucleic Acids Encoding Subunits of Bacterial RNA polymerases
The present invention contemplates isolation of nucleic acids encoding a
subunit of
an RNA polymerase including a full length, i. e., naturally occurring form of
the RNA
polymerase from any prokaryotic source, preferably a thermophilic bacterial
source. The
3 o present invention further provides for subsequent modification of the
nucleic acid to
generate a fragment or modification of the subunit that can still be used to
form a core RNA
polymerase that will crystallize.
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In accordance with the present invention there may be employed conventional
molecular biology, microbiology, and recombinant DNA techniques within the
skill of the
art. Such techniques are explained fully in the literature [see, e.g.,
Sambrook and Russell
Molecular Cloraing.~ A Labo~atofy Manual, Third Edition (2001) Vols. I-III,
Cold Spring
s Harbor Laboratory Press, Cold Spring Harbor, New York (herein "Sambrook and
Russell,
2001 ")].
Therefore, if appearing herein, the following terms shall have the definitions
set out
below.
As used herein, the term "gene" refers to an assembly of nucleotides that
encode a
Z o polypeptide, and includes cDNA and genomic DNA nucleic acids.
A "vector" is a replicon, such as plasmid, phage or cosmid, to which another
DNA
segment may be attached so as to bring about the replication of the attached
segment.
A "replicon" is any genetic element (e.g., plasmid, chromosome, virus) that
functions as an autonomous unit of DNA replication i~ vivo, i. e., capable of
replication
i s under its own control.
A "cassette" refers to a segment of DNA that can be inserted into a vector at
specific
restriction sites. The segment of DNA encodes a polypeptide of interest, and
the cassette
and restriction sites are designed to ensure insertion of the cassette in the
proper reading
frame for transcription and translation.
z o A cell has been "transfected" by exogenous or heterologous DNA when such
DNA
has been introduced inside the cell. A cell has been "transformed" by
exogenous or
heterologous DNA when the transfected DNA effects a phenotypic change.
Preferably, the
transforming DNA should be integrated (covalently linked) into chromosomal DNA
making
up the genome of the cell.
z s "Heterologous DNA" refers to DNA not naturally located in the cell, or in
a
chromosomal site of the cell. Preferably, the heterologous DNA includes a gene
foreign to
the cell.
A "heterologous nucleotide sequence" as used herein is a nucleotide sequence
that is
added to a nucleotide sequence of the present invention by recombinant methods
to form a
s o nucleic acid which is not naturally formed in nature. Such nucleic acids
can encode
chimeric and/or fusion proteins. Thus the heterologous nucleotide sequence can
encode
peptides and/or proteins which contain regulatory and/or structural
properties. In another
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such embodiment the heterologous nucleotide can encode a protein or peptide
that functions
as a means of detecting the protein or peptide encoded by the nucleotide
sequence of the
present invention after the recombinant nucleic acid is expressed. In still
another
embodiment the heterologous nucleotide can function as a means of detecting a
nucleotide
s sequence of the present invention. A heterologous nucleotide sequence can
comprise non
coding sequences including restriction sites, regulatory sites, promoters and
the like.
A "nucleic acid molecule" refers to the phosphate ester polymeric form of
ribonucleosides (adenosine, guanosine, uridine or cytidine; "RNA molecules")
or
deoxyribonucleosides (deoxyadenosine, deoxyguanosine, deoxythymidine, or
i o deoxycytidine; "DNA molecules"), or any phosphoester analogs thereof, such
as
phosphorothioates and thioesters, in either single stranded form, or a double-
stranded helix.
Double stranded DNA-DNA, DNA-RNA and RNA-RNA helices are possible. The term
nucleic acid molecule, and in particular DNA or RNA molecule, refers only to
the primary
and secondary structure of the molecule, and does not limit it to any
particular tertiary
15 forms. Thus, this term includes double-stranded DNA found, inter alia, in
linear or circular
DNA molecules (e.g., restriction fragments), plasmids, and chromosomes. In
discussing the
structure of particular double-stranded DNA molecules, sequences may be
described herein
according to the normal convention of giving only the sequence in the 5' to 3'
direction
along the nontranscribed strand of DNA (i.e., the strand having a sequence
homologous to
a o the rnRNA). A "recombinant DNA molecule" is a DNA molecule that has
undergone a
molecular biological manipulation.
A nucleic acid molecule is "hybridizable" to another nucleic acid molecule,
such as
a cDNA, genomic DNA, or RNA, when a single stranded form of the nucleic acid
molecule
can anneal to the other nucleic acid molecule under the appropriate conditions
of
25 temperature and solution ionic strength [see Sambrook and Russell, 2001,
supra]. The
conditions of temperature and ionic strength determine the "stringency" of the
hybridization. For preliminary screening for homologous nucleic acids, low
stringency
hybridization conditions, corresponding to a Tm of 55°C, can be used,
e.g., Sx SSC, 0.1%
SDS, 0.25% milk, and no formamide; or 30% formamide, Sx SSC, 0.5% SDS).
Moderate
3 o stringency hybridization conditions correspond to a higher Tm, e.g., 40%
formamide, with
Sx or 6x SSC. High stringency hybridization conditions correspond to the
highest Tm, e.g.,
50% fonnamide, Sx or 6x SSC. Hybridization requires that the two nucleic acids
contain
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complementary sequences, although depending on the stringency of the
hybridization,
mismatches between bases are possible. The appropriate stringency for
hybridizing nucleic
acids depends on the length of the nucleic acids and the degree of
complementation,
variables well known in the art. The greater the degree of similarity or
homology between
s two nucleotide sequences, the greater the value of Tm for hybrids of nucleic
acids having
those sequences. The relative stability (corresponding to higher Tm) of
nucleic acid
hybridizations decreases in the following order: RNA:RNA, DNA:RNA, DNA:DNA.
For
hybrids of greater than 100 nucleotides in length, equations for calculating
Tm have been
derived [see Sambrook and Russell, 2001, supra]. For hybridization with
shorter nucleic
s o acids, i. e., oligonucleotides, the position of mismatches becomes more
important, and the
length of the oligonucleotide determines its specificity [see Sambrook and
Russell, 2001,
supra]. Preferably a minimum length for a hybridizable nucleic acid is at
least about 12
nucleotides; preferably at least about 1 ~ nucleotides; and more preferably
the length is at
least about 27 nucleotides; and most preferably 36 nucleotides.
15 In a specific embodiment, the term "standard hybridization conditions"
refers to a
Tm of 55°C, and utilizes conditions as set forth above. In a preferred
embodiment, the Tm is
60°C; in a more preferred embodiment, the Tm is 65°C. In a
particular embodiment the
hybridization and wash conditions are identical,
"Homologous recombination" refers to the insertion of a foreign DNA sequence
of a
z o vector in a chromosome. Preferably, the vector targets a specific
chromosomal site for
homologous recombination. For specific homologous recombination, the vector
will
contain sufficiently long regions of homology to sequences of the chromosome
to allow
complementary binding and incorporation of the vector into the chromosome.
Longer
regions of homology, and greater degrees of sequence similarity, may increase
the
z s efficiency of homologous recombination.
A DNA "coding sequence" is a double-stranded DNA sequence which is transcribed
and translated into a polypeptide in a cell in vitro or ira vivo when placed
under the control
of appropriate regulatory sequences. The boundaries of the coding sequence are
determined
by a start codon at the 5' (amino) terminus and a translation stop codon at
the 3' (carboxyl)
3 o terminus. A coding sequence can include, but is not limited to,
prokaryotic sequences,
cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic (e.g.,
mammalian) DNA, and even synthetic DNA sequences. If the coding sequence is
intended
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for expression in a eukaryotic cell, a polyadenylation signal and
transcription termination
sequence will usually be located 3' to the coding sequence.
Transcriptional and translational control sequences are DNA regulatory
sequences,
such as promoters, enhancers, terminators, and the like, that provide for the
expression of a
s coding sequence in a host cell. In eukaryotic cells, polyadenylation signals
are control
sequences.
A "promoter sequence" is a DNA regulatory region capable of binding RNA
polymerase in a cell and initiating transcription of a downstream (3'
direction) coding
sequence. For purposes of defining the present invention, the promoter
sequence is
1 o bounded at its 3' terminus by the transcription initiation site and
extends upstream (5'
direction) to include the minimum number of bases or elements necessary to
initiate
transcription at levels detectable above background. Within the promoter
sequence will be
found a transcription initiation site (conveniently defined for example, by
mapping with
nuclease S 1), as well as protein binding domains (consensus sequences)
responsible for the
i s binding of RNA polymerase.
A coding sequence is "under the control" of transcriptional and translational
control
sequences in a cell when RNA polymerase transcribes the coding sequence into
mRNA,
which may then be trans-RNA spliced and translated into the protein encoded by
the coding
sequence.
a o As used herein, the term "sequence homology" in all its grammatical forms
refers to
the relationship between proteins that possess a "common evolutionary origin,"
including
proteins from superfamilies (e.g., the immunoglobulin superfamily) and
homologous
proteins from different species (e.g., myosin light chain, etc.) [Reeck et
al., Cell, 50:667
(1987)].
25 Accordingly, the term "sequence similarity" in all its grammatical forms
refers to the
degree of identity or correspondence between nucleic acid or amino acid
sequences of
proteins that do not share a common evolutionary origin [see Reeck et al.,
1987, supra].
However, in common usage and in the instant application, the term
"homologous," when
modified with an adverb such as "highly," may refer to sequence similarity and
not a
s o common evolutionary origin.
In a specific embodiment, two DNA sequences are "substantially homologous" or
"substantially similar" when at least about 50% (preferably at least about
75%, and most
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preferably at least about 90 or 95%) of the nucleotides match over the defined
length of the
DNA sequences. Sequences that are substantially homologous can be identified
by
comparing the sequences using standard software available in sequence data
banks, or in a
Southern hybridization experiment under, for example, stringent conditions as
defined for
s that particular system. Defining appropriate hybridization conditions is
within the skill of
the art. See, e.g., Sambrook and Russell, 2001, supra. Similarly, in a
particular
embodiment, two amino acid sequences are "substantially homologous" or
"substantially
similar" when greater than 30% of the amino acids are identical, or greater
than about 60%
are similar (functionally identical). Preferably, the similar or homologous
sequences are
1 o identified by alignment using, for example, the GCG (Genetics Computer
Group, Program
Manual for the GCG Package, Version 7, Madison, Wisconsin) pileup program with
the
default parameters.
The term "corresponding to" is used herein to refer similar or homologous
sequences, whether the exact position is identical or different from the
molecule to which
is the similarity or homology is measured. Thus, the term "corresponding to"
refers to the
sequence similarity, and not the numbering of the amino acid residues or
nucleotide bases.
A gene encoding an RNA polymerise, including genomic DNA or cDNA, can be
isolated from any source, particularly from a thermophilic bacterial source.
In view and in
conjunction with the present teachings, methods well known in the art, as
described above
a o can be used for obtaining the genes encoding an RNA polymerise from any
source [see,
e.g., Sambrook and Russell, 2001, supra].
Accordingly, any cell potentially can serve as the nucleic acid source for the
molecular cloning of a gene encoding RNA polymerise. The DNA may be obtained
by
standard procedures known in the art from cloned DNA (e.g., a DNA "library"),
and
25 preferably is obtained from a cDNA library, by cDNA cloning, or by the
cloning of genomic
DNA, or fragments thereof, purified from the desired cell [See, for example,
Sambrook and
Russell, 2001,supra]. Clones derived from genomic DNA may contain regulatory
and
intron DNA regions in addition to coding regions; clones derived from cDNA
will not
contain intron sequences. Whatever the source, the gene should be molecularly
cloned into
s o a suitable vector for propagation of the gene.
The present invention also relates to cloning vectors containing genes
encoding
analogs and derivatives of RNA polymerise including and fragments of the
various
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subunits, that can form active forms of RNA polymerase. Included are homologs
of RNA
polymerase and fragments thereof, from other species. Therefore the production
and use of
derivatives and analogs related to RNA polymerase are within the scope of the
present
invention.
s RNA polymerase derivatives can be made by altering encoding nucleic acid
sequences by substitutions, additions or deletions including to provide for
functionally
equivalent molecules. Preferably, derivatives are made that are capable of
forming crystals
with ligands (e.g., inhibitors) of the RNA polymerase with the crystals
capable of
effectively diffracting X-rays for the determination of the atomic coordinates
of the
i o protein-ligand complex to a resolution of better than 5.0 Angstroms,
preferably to a
resolution equal to or better than 3.5 Angstroms.
Due to the degeneracy of nucleotide coding sequences, other DNA sequences
which
encode substantially the same amino acid sequence as a RNA polymerase gene may
be used
in the practice of the present invention. These include but are not limited to
allelic genes,
i5 homologous genes from other species, and nucleotide sequences comprising
all or portions
of RNA polymerase genes which are altered by the substitution of different
codons that
encode the same amino acid residue within the sequence, thus producing a
silent change.
Likewise, the RNA polymerase derivatives of the invention include, but are not
limited to,
those containing, as a primary amino acid sequence, all or part of the amino
acid sequence
2 0 of a RNA polymerase including altered sequences in which functionally
equivalent amino
acid residues are substituted for residues within the sequence resulting in a
conservative
amino acid substitution. For example, one or more amino acid residues within
the sequence
can be substituted by another amino acid of a similar polarity, which acts as
a functional
equivalent, resulting in a silent alteration. Substitutes for an amino acid
within the
a s sequence may be selected from other members of the class to which the
amino acid belongs.
For example, the nonpolar (hydrophobic) amino acids include alanine, leucine,
isoleucine,
valine, proline, phenylalanine, tryptophan and methionine. Amino acids
containing
aromatic ring structures are phenylalanine, tryptophan, and tyrosine. The
polar neutral
amino acids include glycine, serine, threonine, cysteine, tyrosine,
asparagine, and
s o glutamine. The positively charged (basic) amino acids include arginine,
lysine and
histidine. The negatively charged (acidic) amino acids include aspartic acid
and glutamic
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acid. Such alterations will not be expected to affect apparent molecular
weight as
determined by polyacrylamide gel electrophoresis, or isoelectric point.
Particularly preferred substitutions are:
- Lys for Arg and vice versa such that a positive charge may be maintained;
- Glu for Asp and vice versa such that a negative charge may be maintained;
- Ser for Thr such that a free -OH can be maintained; and
- Gln for Asn such that a free NHZ can be maintained.
Amino acid substitutions may also be introduced to substitute an amino acid
with a
particularly preferable property. For example, a Cys may be introduced at a
potential site
s o for disulfide bridges with another Cys. A His may be introduced as a
particularly
"catalytic" site (i. e., His can act as an acid or base and is the most common
amino acid in
biochemical catalysis). Pro may be introduced because of its particularly
planar structure,
which induces (3-turns in the protein's structure.
Such amino acid substitutions, particularly one or more conservative amino
acid
substitutions, may be used in efforts to identify a modulator to RNAP, or more
particularly,
a RNAP binding site in other proteins. For example, conservative amino acid
substitutions
of those found in a RNAP binding site could produce a functionally equivalent
site in
another protein, possibly even non-RNAP protein.
The genes encoding RNA polymerase derivatives and analogs of the invention can
z o be produced by various methods known in the art. The manipulations which
result in their
production can occur at the gene or protein level. For example, the cloned RNA
polymerase gene sequence can be modified by any of numerous strategies known
in the art
[Sambrook and Russell, 2001, supra]. The sequence can be cleaved at
appropriate sites
with restriction endonuclease(s), followed by further enzymatic modification
if desired,
z5 isolated, and ligated in vitYO. In the production of the gene encoding a
derivative or analog
of RNA polymerase, care should be taken to ensure that the modified gene
remains within
the same translational reading frame as the RNA polymerase gene, uninterrupted
by
translational stop signals, in the gene region where the desired activity is
encoded.
Additionally, the RNA polymerase-encoding nucleic acid sequence can be mutated
3 o ira vitf°o or i~z vivo, to create and/or destroy translation,
initiation, and/or termination
sequences, or to create variations in coding regions and/or form new
restriction
endonuclease sites or destroy preexisting ones, to facilitate further ira
vitro modification.
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Preferably, such mutations enhance the functional activity and crystallization
properties of
the mutated RNA polymerase gene product. Any technique for mutagenesis known
in the
art can be used, including but not limited to, in vitro site-directed
mutagenesis [Hutchinson,
et al., J.Biol.Claem. 253:6551 (1978); Zoller and Smith, DNA 3:479-488 (1984);
Oliphant et
s al., Gene 44:177 (1986); Hutchinson et al., Proc.Natl.Acad.Sci. U.S.A.
83:710 (1986)], use
of TAB linkers (Pharmacia), etc. PCR techniques are preferred for site
directed
mutagenesis [see Higuchi,"Using PCR to Engineer DNA", in PCR Teclafaology:
Principles
and Applications for DNA Amplification, H. Erlich, ed., Stockton Press,
Chapter 6, pp. 61-
70 (1989)].
i o The identified and isolated gene can then be inserted into an appropriate
cloning
vector. A large number of vector-host systems known in the art may be used.
Possible
vectors include, but are not limited to, plasmids or modified viruses, but the
vector system
must be compatible with the host cell used. Examples of vectors include, but
are not
limited to, E. coli, bacteriophages such as lambda derivatives, or plasmids
such as pBR322
15 derivatives or pUC plasmid derivatives, e.g., pGEX vectors, pmal-c, pFLAG,
etc. The
insertion into a cloning vector can, for example, be accomplished by ligating
the DNA
fragment into a cloning vector which has complementary cohesive termini.
However, if the
complementary restriction sites used to fragment the DNA are not present in
the cloning
vector, the ends of the DNA molecules may be enzymatically modified.
Alternatively, any
a o site desired may be produced by ligating nucleotide sequences (linkers)
onto the DNA
termini; these ligated linkers may comprise specific chemically synthesized
oligonucleotides encoding restriction endonuclease recognition sequences.
Recombinant
molecules can be introduced into host cells via transformation, transfection,
infection,
electroporation, etc., so that many copies of the gene sequence are generated.
Preferably,
z s the cloned gene is contained on a shuttle vector plasmid, which provides
for expansion in a
cloning cell, e.g., E. coli, and facile purification for subsequent insertion
into an appropriate
expression cell line, if such is desired. For example, a shuttle vector, which
is a vector that
can replicate in more than one type of organism, can be prepared for
replication in both E.
coli and Sacclaaromyces cerevisiae by linking sequences from an E. coli
plasmid with
3 o sequences from the yeast 2p, plasmid.
In an alternative method, the desired gene may be identified and isolated
after
insertion into a suitable cloning vector in a "shot gun" approach. Enrichment
for the desired
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gene, for example, by size fractionation, can be done before insertion into
the cloning
vector.
Expression of RNA Polymerase
The nucleotide sequence coding for RNA polymerase, a fragment of RNA
polymerase or a derivative or analog thereof, including a functionally active
derivative, such
as a chimeric protein, thereof, can be inserted into an appropriate expression
vector, i.e., a
vector which contains the necessary elements for the transcription and
translation of the
inserted protein-coding sequence. Such elements are termed herein a
"promoter." Thus, the
nucleic acid encoding a RNA polymerase of the invention or a fragment thereof
is
s o operationally associated with a promoter in an expression vector of the
invention. Both
cDNA and genomic sequences can be cloned and expressed under control of such
regulatory sequences. An expression vector also preferably includes a
replication origin.
The necessary transcriptional and translational signals can be provided on a
recombinant expression vector, or they may be supplied by the native gene
encoding RNA
15 polymerase and/or its flanking regions.
Potential host-vector systems include but are not limited to mammalian cell
systems
infected with virus (e.g., vaccinia virus, adenovirus, etc.); insect cell
systems infected with
virus (e.g., baculovirus); microorganisms such as yeast containing yeast
vectors; or bacteria
transformed with bacteriophage, DNA, plasmid DNA, or cosmid DNA. The
expression
a o elements of vectors vary in their strengths and specificities. Depending
on the host-vector
system utilized, any one of a number of suitable transcription and translation
elements may
be used.
A recombinant RNA polymerase protein of the invention, or RNA polymerase
fragment, derivative, chimeric construct, or analog thereof, may be expressed
a s chromosomally, after integration of the coding sequence by recombination.
In this regard,
any of a number of amplification systems may be used to achieve high levels of
stable gene
expression [See Sambrook and Russell, 2001, supT~a].
The cell containing the recombinant vector comprising the nucleic acid
encoding
RNA polymerase is cultured in an appropriate cell culture medium under
conditions that
3 o provide for expression of RNA polymerase by the cell.
Any of the methods previously described for the insertion of DNA fragments
into a
cloning vector may be used to construct expression vectors containing a gene
consisting of
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CA 02440409 2003-09-09
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appropriate transcriptional/translational control signals and the protein
coding sequences.
These methods may include izz viti~o recombinant DNA and synthetic techniques
and izz vivo
recombination (genetic recombination).
Expression of RNA polymerise may be controlled by any promoter/enhancer
s element known in the art, but these regulatory elements must be functional
in the host
selected for expression. Promoters that may be used to control RNA polymerise
gene
expression are well known in the art including prokaryotic expression vectors
such as the /3-
lactamase promoter [Villa-Kamaroff, et al., Proc. Natl. Acid. Sci. U.S.A.,
75:3727-3731
(1978)], or the tic promoter [DeBoer, et al., P>~oc. Natl. Acid. Sci. U.S.A.,
80:21-25
io (1983)].
Expression vectors containing a nucleic acid encoding an RNA polymerise of the
invention can be identified by a number of means including four general
approaches: (a)
PCR amplification of the desired plasmid DNA or specific mRNA, (b) nucleic
acid
hybridization, (c) presence or absence of selection marker gene functions, and
(d)
i5 expression of inserted sequences. In the first approach, the nucleic acids
can be amplified
by PCR to provide for detection of the amplified product. In the second
approach, the
presence of a foreign gene inserted in an expression vector can be detected by
nucleic acid
hybridization using probes comprising sequences that are homologous to an
inserted marker
gene. In the third approach, the recombinant vector/host system can be
identified and
~ o selected based upon the presence or absence of certain "selection marker"
gene functions
(e.g., (3-galactosidase activity, thymidine kinase activity, resistance to
antibiotics,
transformation phenotype, occlusion body formation in baculovirus, etc.)
caused by the
insertion of foreign genes in the vector. In another example, if the nucleic
acid encoding
RNA polymerise is inserted within the "selection marker" gene sequence of the
vector,
a s recombinants containing the RNA polymerise insert can be identified by the
absence of the
selection marker gene function. In the fourth approach, recombinant expression
vectors can
be identified by assaying for the activity, biochemical, or immunological
characteristics of
the RNA polymerise expressed by the recombinant, provided that the expressed
protein
assumes a functionally active conformation.
3 o A wide variety of host/expression vector combinations may be employed in
expressing the DNA sequences of this invention. Useful expression vectoxs, for
example,
may consist of segments of chromosomal, non-chromosomal and synthetic DNA
sequences.
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Suitable vectors include derivatives of SV40 and known bacterial plasmids,
e.g., E. coli
plasmids col El, pCRl, pBR322, pMal-C2, pET, pGEX [Smith et al., Gene, 67:31-
40
(1988)], pMB9 and their derivatives, plasmids such as RP4; phage DNAS, e.g.,
the
numerous derivatives of phage 7~, e.g., NM989, and other phage DNA, e.g., M13
and
s filarnentous single stranded phage DNA; yeast plasmids such as the 2w
plasmid or
derivatives thereof; vectors useful in eukaryotic cells, such as vectors
useful in insect or
mammalian cells; vectors derived from combinations of plasmids and phage DNAs,
such as
plasmids that have been modified to employ phage DNA or other expression
control
sequences; and the like.
1 o For example, in a baculovirus expression systems, both non-fusion transfer
vectors,
such as but not limited to pVL941 (BamHl cloning site; Summers), pVL1393
(BanzHl,
SnZaI, XbaI, EcoRl, NotI, XmaIll, Bglll, and PstI cloning site; Invitrogen),
pVL1392 (Bglll,
PstI, NotI, XmallI, EcoRI, XbaI, SmaI, and BamHl cloning site; Summers and
Invitrogen),
and pBlueBacIlI (BamHl, Bglll, PstI, NcoI, and HindIll cloning site, with
blue/white
15 recombinant screening possible; Invitrogen), and fusion transfer vectors,
such as but not
limited to pAc700 (BanaHl and KpnI cloning site, in which the BamHl
recognition site
begins with the initiation codon; Summers), pAc701 and pAc702 (same as pAc700,
with
different reading frames), pAc360 (BamHl cloning site 36 base pairs downstream
of a
polyhedrin initiation codon; Invitrogen(195)), and pBlueBacHisA, B, C (three
different
2 o reading frames, with BamHl, Bglll, PstI, NcoI, and HindIll cloning site,
an N-terminal
peptide for ProBond purification, and blue/white recombinant screening of
plaques;
Invitrogen (220)) can be used.
Mammalian expression vectors contemplated for use in the invention include
vectors with inducible promoters, such as the dihydrofolate reductase (DHFR)
promoter,
2 s e.g., any expression vector with a DHFR expression vector, or a
DHFR/methotrexate co-
amplification vector, such as pED (PstI, SaII, SbaI, SmaI, and EcoRI cloning
site, with the
vector expressing both the cloned gene and DHFR; see Kaufman, Current
Protocols in
Molecular Biology, 16.12 (1991). Alternatively, a glutamine
synthetase/methionine
sulfoximine co-amplification vector, such as pEEl4 (HindIll, XbaI, SnaaI,
SbaI, EcoRI, and
3 o BcII cloning site, in which the vector expresses glutamine synthase and
the cloned gene;
Celltech). In another embodiment, a vector that directs episomal expression
under control
of Epstein Barr Virus (EBV) can be used, such as pREP4 (BamHl, SfiI, XhoI,
NotI, NheI,
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HindIll, NI~eI, PvuII, and KpnI cloning site, constitutive RSV-LTR promoter,
hygromycin
selectable marker; Invitrogen), pCEP4 (BarnHl, SfiI, ~I'laoI, NotI, NlaeI,
HindIll, NheI, PvuII,
and KpnI cloning site, constitutive hCMV immediate early gene, hygromycin
selectable
marker; Tnvitrogen), pMEP4 (KpnI, PvuI, NheI, HiradIll, NotI, XlaoI, SfiI,
BamHl cloning
s site, inducible methallothionein IIa gene promoter, hygromycin selectable
marker:
Invitrogen), pREP8 (BarnHl, XlaoI, NotI, HindIll, NheI, and KpnI cloning site,
RSV-LTR
promoter, histidinol selectable marker; Invitrogen), pREP9 (KpnI, NheI,
HindlB, NotI,
Xh.oI, SfiI, and BamHI cloning site, RSV-LTR promoter, 6418 selectable marker;
Invitrogen), and pEBVHis (RSV-LTR promoter, hygromycin selectable marker, N-
terminal
1 o peptide purifiable via ProBond resin and cleaved by enterokinase;
Invitrogen). Selectable
mammalian expression vectors for use in the invention include pRc/CMV (HindlB,
BstXI,
NotI, SbaI, and ApaI cloning site, 6418 selection; Invitrogen), pRc/RSV
(Hindlll, SpeI,
BstXI, NotI, XbaI cloning site, 6418 selection; Invitrogen), and others.
Vaccinia virus
mammalian expression vectors (see, Kaufman, 1991, supra) for use according to
the
is invention include but are not limited to pSCl l (SmaI cloning site, TK- and
(i-gal selection),
pMJ601 (SaII, SmaI, AfII, NarI, BspMII, BarrzHI, ApaI, N7aeI, SacII, KpraI,
and HindIII
cloning site; TK- and (3-gal selection), and pTKgptF 1 S (EcoRI, PstI, SaII,
AccI, HindII,
SbaI, BamHI, and Hpa cloning site, TK or XPRT selection).
Yeast expression systems can also be used according to the invention to
express the
a o bacterial RNA polyrnerase. For example, the non-fusion pYES2 vector (XbaI,
SpIZI, SlaoI,
NotI, GstXI, EcoRI, BstXI, BanaHl, SacI, Kpnl, and HindIlI cloning sit;
Invitrogen) or the
fusion pYESHisA, B, C (XbaI, SphI, ShoI, NotI, BstXI, EcoRI, BanzHl, SacI,
KpnI, and
HindIII cloning site, N-terminal peptide purified with ProBond resin and
cleaved with
enterokinase; Invitrogen), to mention just two, can be employed according to
the invention.
25 Once a particular recombinant DNA molecule is identified and isolated,
several
methods known in the art may be used to propagate it. Once a suitable host
system and
growth conditions are established, recombinant expression vectors can be
propagated and
prepared in quantity. As previously explained, the expression vectors which
can be used
include, but are not limited to, the following vectors or their derivatives:
human or animal
3 o viruses such as vaccinia virus or adenovirus; insect viruses such as
baculovirus; yeast
vectors; bacteriophage vectors (e.g., lambda), and plasmid and cosmid DNA
vectors, to
name but a few.
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CA 02440409 2003-09-09
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Vectors are introduced into the desired host cells by methods known in the
art, e.g.,
transfection, electroporation, microinjection, transduction, cell fusion, DEAF
dextran,
calcium phosphate precipitation, lipofection (lysosome fusion), use of a gene
gun, or a
DNA vector transporter [see, e.g., Wu et al., J. Biol. Chem., 267:963-967
(1992); Wu and
s Wu, J. Biol. Claem., 263:14621-14624 (1988); Hartmut et al., Canadian Patent
Application
No. 2,012,31 l, filed March 15, 1990).
Peptide Synthesis
Synthetic polypeptides, prepared using the well known techniques of solid
phase,
liquid phase, or peptide condensation techniques, or any combination thereof,
can include
i o natural and unnatural amino acids. Amino acids used for peptide synthesis
may be standard
Boc (N°'-amino protected Na-t-butyloxycarbonyl) amino acid resin with
the standard
deprotecting, neutralization, coupling and wash protocols of the original
solid phase
procedure of Merrifield [J. Am. Chern. Soc., 85:2149-2154 (1963)], or the base-
labile Na-
amino protected 9-fluorenylmethoxycarbonyl (Fmoc) amino acids first described
by
i5 Carpino and Han [J. O~g. Cl~em., 37:3403-3409 (1972)]. Both Fmoc and Boc N"-
amino
protected amino acids can be obtained from Fluka, Bachem, Advanced Chemtech,
Sigma,
Cambridge Research Biochemical, Bachem, or Peninsula Labs or other chemical
companies
familiar to those who practice this art. In addition, the method of the
invention can be used
with other Na-protecting groups that are familiar to those skilled in this
art. Solid phase
z o peptide synthesis may be accomplished by techniques familiar to those in
the art and
provided, [e.g., Stewart and Young, Solid Phase Synthesis, Second Edition,
Pierce
Chemical Co., Rockford, IL (1984); Fields and Noble, Ifat. J. Pept. Pf"Oteil2
Res. 35:161-214
(1990)], or using automated synthesizers, such as sold by ABS. Thus,
polypeptides of the
invention may comprise D-amino acids, a combination of D- and L-amino acids,
and
as various "designer" amino acids (e.g., (3-methyl amino acids, Ca-methyl
amino acids, and
Na-methyl amino acids, etc.) to convey special properties. Synthetic amino
acids include
ornithine for lysine, fluorophenylalanine for phenylalanine, and norleucine
for leucine or
isoleucine. Additionally, by assigning specific amino acids at specific
coupling steps, a-
helices, (3 turns, (3 sheets, y-turns, and cyclic peptides can be generated.
3 o Isolation and Crystallization of the Bacterial RNA Polymerase
The present invention provides a crystal of the Rif RNAP complex that can be
effectively diffract X-rays for the determination of the atomic coordinates of
the Rif RNAP
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CA 02440409 2003-09-09
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to a resolution of better than 5.0 Angstroms and preferably to a resolution
equal to or better
than 3.5 Angstroms. The RNA polymerase can be expressed either as described
above or as
described in Zhang et al., Cell 98:811-824 (1999). Of course, the specific Rif
RNAP
complex provided herein serves only as example, since the crystallization
process can
s tolerate a broad range of active RNA polyrnerases and inhibitors. Therefore,
any person
with skill in the art of protein crystallization having the present teachings
and without
undue experimentation could crystallize a large number of alternative forms of
the RNA
polymerases from a variety of RNA polymerase fragments, or alternatively using
a full
length RNA polyrnerase from a related source and then allow the RNA polymerase
to bind
to rifampicin and/or other RNAP binding partners (e.g., inhibitors) as
described below. As
mentioned above, an RNA polymerase having conservative substitutions in its
amino acid
sequence are also included in the invention, including a selenomethionine
substituted form.
Crystals of the RNA polymerase can be grown by a number of techniques
including
batch crystallization, vapor diffusion (either by sitting drop or hanging
drop) and by
is microdialysis. Seeding of the crystals in some instances is required to
obtain X-ray quality
crystals. Standard micro and/or macro seeding of crystals may therefore be
used.
The crystals of the RNA polymerase can be grown alone or co-crystallized with
a
binding partner such as rifampicin. If the crystals are grown alone they can
be subsequently
soaked in a stabilization buffer with an RNAP binding partner such as
rifampicin (0.1 mM
z o rifampicin was added in the Example below). The RNAP/RNAP-binding partner
are
preferably incubated in the stabilization buffer for at least twelve hours. An
exemplary
stabilization buffer contains betweenl.7 - 2.3 M (NH4)ZSO4, 0.02-1 M Tris-HCI,
pH 6.5-
8.5, and approximately 20 mM MgClz.
The crystals are then prepared for cryo-crystallography by soaking the
25 RNAP/RNAP-binding partner complex in a stabilization buffer (e.g., 2 M
(NH4)ZSO4, 0.1
M Tris-HCI, pH 8.0, and 20 mM MgCl2 containing 50% (w/v) sucrose) before flash
freezing.
Aside from the methodology exemplified below, alternative methods may also be
used to characterize the crystals. For example, crystals can be characterized
by using X-
3 o rays produced in a conventional source (such as a sealed tube or a
rotating anode) or using a
synchrotron source. Methods of characterization include, but are not limited
to, precision
photography, oscillation photography and diffractometer data collection.
Selenium-
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CA 02440409 2003-09-09
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Methionine may be used, or alternatively a mercury derivative dataset (e.g.,
using PCMB)
could be used in place of the selenium-methionine derivatization.
Structural determinations can be performed by calculating Patterson maps using
PHASES [Furey and Swaminathan, Methods Enzymol., 277:590-620 (1997)] for the
s ethyl-HgCl2 and Ta6Br14 derivatives and using the Pb-derivative as native,
for example. In
the Example below, the native core RNAP structure [Zhang et al., Cell 98:811-
824 (1999)]
was used as a starting model for rigid body refinement and positional
refinement against the
observed amplitudes from the Rif RNAP complex crystal (Fo 'f:) using CNS
[Adams et al.,
Proc. Natl. Acad. Sci. USA, 94:5018-5023 (1997)], yielding an initial R-factor
of 0.354
l o (Rrree = 0.41, where the same set of reflections was set aside as was used
for the Rfree
determination of the native structure) for data from 100 - 3.21 resolution. An
initial
Fourier difference map, calculated using ~F R'f - Fonatl amplitude
coefficients and using
phases calculated from the native core RNAP structure (cp°at) clearly
revealed density for the
rifampicin molecule (Fig. 3a). Multiple rounds of manual rebuilding against
(2~FoI - ~F~~)
15 maps using O [Jones et al., Acta Cryst, A 47:110-119 (1991)], and
refinement using CNS
[Adams et al., Proc. Natl. Acad. Sei. USA, 94:5018-5023 (1997)] resulted in
the current
model (Table 1 ). At later stages of the refinement, the rifampicin X-ray
crystal structure
[Brufani et al., J. Molec.Biol. 87:409-435 (1974)] was placed into the
difference density.
Included in the model is the recently determined sequence of the Taq co
subunit modeled
2 o earlier as a polyalanine chain [Zhang et al., Cell 98:811-824 (1999)].
Absent from the
model is a 300 amino acid, non-conserved domain inserted between conserved
regions A
and B of the (3' subunit [Zhang et al., Cell 98:811-824 (1999)].
Three dimensional representation
The structure coordinates generated for Taq RNAP or the Taq RNAP/rifampicin
2 s complex or one of its binding pockets shown in Table 2 define a unique
configuration of
points in space. Those of skill in the art understand that a set of structure
coordinates for
protein or a protein/ligand complex, or a portion thereof, define a relative
set of points that,
in turn, define a configuration in three dimensions.
A similar or identical configuration can be defined by an entirely different
set of
3 o coordinates, provided the distances and angles between coordinates remain
essentially the
same. In addition, a scalable configuration of points can be defined by
increasing or
decreasing the distances between coordinates by a scalar factor while keeping
the angles
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essentially the same. The present invention thus includes a scalable three-
dimensional
configuration of points derived from the structure coordinates of at least a
portion of a Taq
RNAP molecule or molecular complex, as shown in Table 2, as well as
structurally
equivalent configurations, as described below. Preferably, the scalable three-
dimensional
s configuration includes points derived from structure coordinates
representing the locations
of a plurality of the amino acids defininga binding pocket, such as the Rif
binding pocket.
In one embodiment, the scalable three-dimensional configuration includes
points derived
from structure coordinates representing the locations the backbone atoms of a
plurality of
amino acids defining the Taq RNAP binding pocket, preferably the amino acids
listed in
1 o Table 3; in another embodiment, the scalable three-dimensional
configuration includes
points derived from structure coordinates representing the locations of the
side chain and
the backbone atoms (other than hydrogens) of a plurality of the amino acids
defining a
binding pocket, preferably the amino acids listed in Table 3.
Likewise, the invention also includes the scalable three-dimensional
configuration
is of points derived from structure coordinates of molecules or molecular
complexes that are
structurally homologous to Taq RNAP, as well as structurally equivalent
configurations.
Structurally homologous molecules or molecular complexes are defined below.
Advantageously, structurally homologous molecules can be identified using the
structure
coordinates of Taq RNAP (Table 2) according to a method of the invention.
z o The configurations of points in space derived from structure coordinates
according
to the invention can be visualized as, for example, a holographic image, a
stereodiagram, a
model or a computer-displayed image, and the invention thus includes such
images,
diagrams or models.
Binding pockets
z5 Binding pockets are of significant utility in fields such as drug
discovery. The
association of natural ligands or substrates with the binding pockets of their
corresponding
receptors or enzymes is the basis of many biological mechanisms of action.
Similarly, many
drugs exert their biological effects through association with the binding
pockets of receptors
and enzymes. Such associations may occur with all or any parts of the binding
pocket. An
s o understanding of such associations helps lead to the design of drugs
having more favorable
associations with their target, and thus improved biological effects.
Therefore, this
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information is valuable in designing potential inhibitors of RNAP-like binding
pockets, as
discussed in more detail below.
The term "binding pocket," as used herein, refers to a region of a molecule or
molecular complex, that, as a result of its shape, favorably associates with
another chemical
s entity or modulator. Exemplary binding pockets include active sites, surface
grooves or
contours or surfaces of a RNAP which are capable of participating in
interactions with
another modulator.
One example of a binding pocket of RNAP includes the amino acids listed in
Table 3; more preferably the amino acids listed in Table 4, corresponding to
the structure
1 o coordinates listed in Table 2. This RNAP binding pocket is referred to
herein as the "Rif
RNAP binding pocket".
Table 3
Amino acids within Rif RNAP binding pocket
i5 Q390 L391 Q393
F394 D396 H406
8409 5411 L413
6414 I452 E445
2 o Table
4
Amino acids pocket
within
Rif RNAP
binding
5387 Q390 L391 5392 Q393 F394
K395 D396 T398 L401 5402 H406
8409 5411 A412 L413 6414 P443
25 P444 I452 T453 T566
It will be readily apparent to those of skill in the art that the numbering of
amino
acids in other isoforms of RNAP may be different than that of RNAP isolated
from Taq. In
another alternative, the binding pocket of RNAP may be defined by those amino
acids
3 o whose backbone atoms are situated within about 4~, more preferably within
about 7~,
most preferably within about 101, of one or more constituent atoms of a bound
substrate or
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inhibitor, as determined from the structure coordinates in Table 2. Yet
another way of
defining the binding pocket of RNAP is in terms of pairwise interatomic
distances.
The amino acid constituents of an RNAP binding pocket as defined herein, as
well
as selected constituent atoms thereof, are positioned in three dimensions in
accordance with
s the structure coordinates listed in Table 2. In one aspect, the structure
coordinates defining
the binding pocket of RNAP include structure coordinates of all atoms in the
constituent
amino acids; in another aspect, the structure coordinates of the binding
pocket include
structure coordinates of just the backbone atoms of the constituent amino
acids; in another
aspect, the structure coordinates of the binding pocket include structure
coordinates of just
i o the side chain atoms (with or withoutCa) of the constituent amino acids.
For all of these
binding pockets, conservative amino acid substitutions may be made, such as
those that
substantially preserve the structure of the binding pocket or the position of
the chemical
characteristics of the side chain atoms (e.g., hydrophilic, hydrophobic,
nucleophilic,
aromatic, charged, etc.)
15 Tn further detail, a RNAP binding pocket refers to a portion of a molecule
or
molecular complex whose shape is sufficiently similar to at least a portion of
the RNAP
substrate binding pocket of RNAP as to be expected to bind related ligands. A
structurally
equivalent binding pocket is defined by a xoot mean square deviation from the
structure
coordinates of the backbone atoms of the amino acids that make up the binding
pockets in
2 o RNAP (as set forth in Table 2) of at most about 1.5~. How this calculation
is obtained is
described below.
One example of such a RNAP-like binding pocket are defined by the amino acids
in
Table 3 or 4. Embodiments of the invention which make use of the binding
pocket can also
use only a portion of the amino acids listed in Table 3 or 4. The number of
amino acids of
z 5 Table 3 or 4 that is sufficient for a particular embodiment will depend on
the embodiment.
For example, in certain embodiment, it will be sufficient to use at least
about 3, 5, 7, 9, or
amino acids of Table 3 or 4, or other amino acids located sufficiently close
to the surface
of the pocket.
Other binding pockets include the DNA binding site; the nucleotide binding
site; the
3 o divalent cation binding site; potential cofactor binding sites; and
protein binding sites (such
as transcription factor binding sites).
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Preferred drug binding sites ("druggable sites") are located within a certain
distance
from binding pockets. Druggable sites can be located within about ltd; 2~; 3~;
4th; 51~;
6th; 7~; 8th; 9~; 10~; 15~; 20~ or 30~, or between two distances (e.g. 1-SEA)
of a binding
pocket. The distance may be calculated from the center of the druggable site
and the
binding pocket. Alternatively, the distance is calculated from one or more
amino acids or
atoms thereof within the druggable site or binding pocket.
For the purpose of this invention, any molecule or molecular complex or
binding
pocket thereof, or any portion thereof, that has a root mean square deviation
of conserved
residue backbone atoms (N, Ca, C, O) of less than about 1.5A, when
superimposed on the
1 o relevant backbone atoms described by the reference structure coordinates
listed in Table 2,
is considered "structurally equivalent' 'to the reference molecule.
Alternatively, the root
mean square deviation is less than about 1.25 or 1.0 ~. That is to say, the
crystal structures
of those portions of the two molecules are substantially identical, within
acceptable error.
Particularly preferred structurally equivalent molecules or molecular
complexes are those
~.5 that are defined by the entire set of structure coordinates in Table 2, +!-
a root mean square
deviation from the conserved backbone atoms of those amino acids of not more
than 1.5~.
The term "root mean square deviation" means the square root of the arithmetic
mean of the squares of the deviations. It is a way to express the deviation or
variation from
a trend or object. For purposes of this invention, the "root mean square
deviation" defines
a o the variation in the backbone of a protein from the backbone of RNAP or a
binding pocket
portion thereof, as defined by the structure coordinates of RNAP described
herein.
Alternatively, in certain instances when expressly indicated, the "root mean
square
deviation" may refer to the side chain atoms (including for this purpose the
Ca but not the
N, C, O).
Structurally Homologous Molecules, Molecular Complexes, And Crystal Structures
The structure coordinates set forth in Table 2 can be used to aid in obtaining
structural information about another crystallized molecule or molecular
complex. A
"molecular complex" means a protein in covalent or non-covalent association
with a
3 o chemical entity or compound. The method of the invention allows
determination of at least
a portion of the three-dimensional structure of molecules or molecular
complexes which
contain one or more structural features that are similar to structural
features of RNAP.
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These molecules are referred to herein as structurally homologous" to Taq
RNAP. Similar
structural features can include, for example, regions of amino acid identity,
structural
motifs, conserved active site or binding site motifs, and similarly arranged
secondary
structural elements (e.g., a helices and (3 sheets).
s Optionally, structural homology is determined by aligning the residues of
the two
amino acid sequences to optimize the number of identical amino acids along the
lengths of
their sequences; gaps in either or both sequences are permitted in making the
alignment in
order to optimize the number of identical amino acids, although the amino
acids in each
sequence must nonetheless remain in their proper order. Preferably, two amino
acid
to sequences are compared using the Blastp program, version 2Ø9, of the
BLAST 2 search
algorithm, as described by Tatusova et al., FEMS Microbiol Lett 174, 247-50
(1999), and
available at http://www.ncbi.nlm.nih.gov/gorflbl2.html. Preferably, the
default values for
all BLAST 2 search parameters are used, including matrix = BLOSUM62; open gap
penalty
= 1 l, extension gap penalty = 1, gap X dropoff = 50, expect = 10, wordsize =
3, and filter
i5 on. In the comparison of two amino acid sequences using the BLAST search
algorithm,
structural similarity is referred to as "identity." Preferably, a structurally
homologous
molecule is a protein that has an amino acid sequence sharing at least 65%
identity with the
amino acid sequence of Taq RNAP (SEQ ID NO: 2). More preferably, a protein
that is
structurally homologous to Taq RNAP includes at least one contiguous stretch
of at least 50
z o amino acids that shares at least 80% amino acid sequence identity with the
analogous
portion of Taq RNAP. Methods for generating structural information about the
structurally
homologous molecule or molecular complex are well-known and include, for
example,
molecular replacement techniques.
Therefore, in another embodiment this invention provides a method of utilizing
as molecular replacement to obtain structural information about a molecule or
molecular
complex whose structure is unknown comprising the steps of (a) crystallizing
the molecule
or molecular complex of unknown structure; (b) generating an x-ray diffraction
pattern
from said crystallized molecule or molecular complex; and (c) applying at
least a portion of
the structure coordinates set forth in Table 2 to the x-ray diffraction
pattern to generate a
3 o three-dimensional electron density map of the molecule or molecular
complex whose
structure is unknown.
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By using molecular replacement, all or part of the structure coordinates of
Taq
RNAP or the Taq RNAP/ligand complex as provided by this invention (and set
forth in
Table 2) can be used to determine the structure of a crystallized molecule or
molecular
complex whose structure is unknown more quickly and efficiently than
attempting to
s determine such information ab initio.
Molecular replacement provides an accurate estimation of the phases for an
unknown structure. Phases are a factor in equations used to solve crystal
structures that
cannot be determined directly. Obtaining accurate values for the phases, by
methods other
than molecular replacement, is a time-consuming process that involves
iterative cycles of
1 o approximations and refinements and greatly hinders the solution of crystal
structures.
However, when the crystal structure of a protein containing at least a
structurally
homologous portion has been solved, the phases from the known structure
provide a
satisfactory estimate of the phases for the unknown structure.
Thus, this method involves generating a preliminary model of a molecule or
is molecular complex whose structure coordinates are unknown, by orienting and
positioning
the relevant portion of Taq RNAP or the Taq RNAP/ligand complex according to
Table 2
within the unit cell of the crystal of the unknown molecule or molecular
complex so as best
to account for the observed x-ray diffraction pattern of the crystal of the
molecule or
molecular complex whose structure is unknown. Phases can then be calculated
from this
2 o model and combined with the observed x-ray diffraction pattern amplitudes
to generate an
electron density map of the structure whose coordinates are unknown. This, in
turn, can be
subjected to any wellknown model building and structure refinement techniques
to provide
a final, accurate structure of the unknown crystallized molecule or molecular
complex (E.
Lattman, "Use of the Rotation and Translation Functions," in Meth. Enzymol.,
115, pp. 55-
25 77 (1985); M.G. Rossman, ed., "The Molecular Replacement Method," Int. Sci.
Rev. Ser.,
No. 13, Gordon & Breach, New York (1972)).
Structural information about a portion of any crystallized molecule or
molecular
complex that is sufficiently structurally homologous to a portion of Taq RNAP
can be
resolved by this method. In addition to a molecule that shares one or more
structural
3 o features with Taq RNAP as described above, a molecule that has similar
bioactivity, such as
the same catalytic activity, substrate specificity or ligand binding activity
as Taq RNAP may
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also be sufficiently structurally homologous to Taq RNAP to permit use of the
structure
coordinates of Taq RNAP to solve its crystal structure.
In a preferred embodiment, the method of molecular replacement is utilized to
obtain structural information about a molecule or molecular complex, wherein
the molecule
s or molecular complex comprises at least one Taq RNAP subunit or homolog. A
"subunit"
of RNAP can be subunit a, (i, (3', to, or 6, or it can be an RNAP molecule
that has been
truncated at the N-terminus or the C-terminus, or both. In the context of the
present
invention, a "homolog" of a Taq RNAP or subunit thereof is a protein complex
or subunit
thereof, respectively, that contains one or more amino acid substitutions,
deletions,
1 o additions, or rearrangements with respect to the amino acid sequence of
Taq RNAP or
subunit thereof, but that, when folded into its native conformation, exhibits
or is reasonably
expected to exhibit at least a portion of the tertiary (three-dimensional)
structure of Taq
RNAP or subunit thereof. For example, structurally homologous molecules can
contain
deletions or additions of one or more contiguous or noncontiguous amino acids,
such as a
i5 loop or a domain. Structurally homologous molecules also include "modified"
Taq RNAP
molecules or subunit thereof that have been chemically or enzymatically
derivatized at one
or more constituent amino acid, including side chain modifications, backbone
modifications, and N- and C- terminal modifications including acetylation,
hydroxylation,
methylation, amidation, and the attachment of carbohydrate or lipid moieties,
cofactors, and
z o the like.
A heavy atom derivative of Taq RNAP is also included as a Taq RNAP homolog.
The term "heavy atom derivative" refers to derivatives of Taq RNAP produced by
chemically modifying a crystal of Taq RNAP. In practice, a crystal is soaked
in a solution
containing heavy metal atom salts, or organometallic compounds, e.g., lead
chloride, gold
z s thionialate, thiomersal or uranyl acetate, which can diffuse through the
crystal and bind to
the surface of the protein. The locations) of the bound heavy metal atoms) can
be
determined by x-ray diffraction analysis of the soaked crystal. This
information, in turn, is
used to generate the phase information used to construct three-dimensional
structure of the
protein (T.L. Blundell and N.L. Johnson, Protein Crystallogrgphy Academic
Press (1976)).
3 o Because Taq RNAP can crystallize in more than one crystal form, the
structure
coordinates of Taq RNAP as provided by this invention are particularly useful
in solving
the structure of other crystal forms of Taq RNAP or Taq RNAP complexes.
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The structure coordinates of Taq RNAP in Table 2 are also particularly useful
to
solve the structure of crystals of Taq RNAP homologs, Taq RNAP mutants, or Taq
RNAP
homologs co-complexed with a variety of chemical entities. This approach
enables the
determination of the optimal sites for interaction between chemical entities,
including
s candidate Taq RNAP inhibitors and Taq RNAP. Potential sites for modification
within the
various binding site of the molecule can also be identified. This information
provides an
additional tool for determining the most efficient binding interactions, for
example,
increased hydrophobic interactions, between Taq RNAP and a chemical entity.
For
example, high resolution x-ray diffraction data collected from crystals
exposed to different
i o types of solvent allows the determination of where each type of solvent
molecule resides.
Small molecules or other modulators that bind tightly to those sites can then
be designed
and synthesized and tested for their Taq RNAP inhibition activity.
All of the complexes referred to above may be studied using well-known x-ray
diffraction techniques and may be refined versus 1.5-31~ resolution x-ray data
to an R value
i5 of about 0.20 or less using computer software, such as X-PLOR (Yale
University, (1992),
distributed by Molecular Simulations, Inc.; see, e.g., Blundell & Johnson, ;
Meth. Enzymol.,
Vol. 114 & 115, H.W. Wyckoff et al., eds., Academic Press (1985)). This
information may
thus be used to optimize known Taq RNAP inhibitors, and more importantly, to
design new
Taq RNAP inhibitors.
a o The invention also includes the unique scalable three-dimensional
configuration
defined by a set of points defined by the structure coordinates for a molecule
or molecular
complex structurally homologous to Taq RNAP as determined using the method of
the
present invention, structurally equivalent configurations, and magnetic
storage media
comprising such set of structure coordinates.
25 Further, the invention includes structurally homologous molecules as
identified
using the method of the invention.
Homoloey Modeling
Using homology modeling, a computer model of a Taq RNAP homolog can be
built or refined without crystallizing the homolog. First, a preliminary model
of the Taq
3 o RNAP homolog is created by sequence alignment with Taq RNAP, secondary
structure
prediction, the screening of structural libraries, or any combination of those
techniques.
Computational software may be used to carry out the sequence alignments and
the
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secondary structure predictions. Structural incoherences, e.g., structural
fragments around
insertions and deletions, can be modeled by screening a structural library for
peptides of the
desired length and with a suitable conformation.
For prediction of the side chain conformation, a side chain rotamer library
may be
s employed. Where the Taq RNAP homolog has been crystallized, the final
homology model
can be used to solve the crystal structure of the homolog by molecular
replacement, as
described above. Next, the preliminary model is subjected to energy
minimization to yield
an energy minimized model. The energy minimized model may contain regions
where
stereochemistry restraints are violated, in which case such regions are
remodeled to obtain a
~. o anal homology model. The homology model is positioned according to the
results of
molecular replacement, and subjected to further refinement comprising
molecular
dynamics calculations.
Protein-structure Based Design of Inhibitors of Bacterial RNA Polymerase
Once the three-dimensional structure of a crystal comprising a Rif RNAP
complex
15 is determined, (e.g., see the coordinates in Table 2 below, in Appendix
following the
Sequence Listing) a potential modulator of RNA Polymerase, can be examined
through the
use of computer modeling using a docking program such as GRAM, DOCK, or
AUTODOCK [Dunbrack et al., Folding ds I~esigh, 2:27-42 (1997)], to identify
potential
modulators of the RNA Polymerase. This procedure can include computer fitting
of
z o potential modulators to the RNA Polymerase to ascertain how well the shape
and the
chemical structure of the potential modulator will bind to either the
individual bound
subunits or to the RNA Polymerase [Bugg et al., Scientific American, Dec.:92-
98 (1993);
West et al., TIPS, 16:67-74 (1995)]. Computer programs can also be employed to
estimate
the attraction, repulsion, and steric hindrance of the subunits with a
modulator/inhibitor
z 5 (e.g., the RNA Polymerase and a potential stabilizer).
Indeed, the shape of RNA polymerase resembles a crab-claw, with an internal
groove or channel running along the full-length (between the claws). The
molecule is about
150 A long (from the back to the tips of the claws), 115 ~ tall, and 110 A
wide (along the
direction of the channel). The channel has many internal features, but the
overall width is
s o about 27 ~ [see, Zhang et al., Cell 98:811-824 (1999)].
As disclosed herein the three-dimensional structure demonstrates that
rifampicin
binds the Taq core RNAP with a close complementary fit in a pocket between two
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structural domains of the RNAP ~ subunit. Only small, local conformational
changes of
both the inhibitor and the protein is observed. The binding site is deep
within the main
RNAP channel, but the closest approach of the inhibitor to the RNAP active
site Mg2+ is
more than 121.
s Importantly, the structural information disclosed herein demonstrates that
rifampicin
inhibits RNA polymerase by physically blocking transcription elongation. This
is in direct
contrast with the »todus opera>zdi of a classical enzyme inhibitor which
generally binds to
the catalytic center or with a key transition state intermediate. Therefore,
the effect of
rifampicin depends only on its ability to bind tightly to a relatively non-
conserved part of
1 o the structure, thereby disrupting a critical RNAP function. Thus, the
structural information
disclosed herein provides the impetus to investigate the binding of other
unrelated small
molecules to any of a variety of sites within the RNAP channel, which could
also block
transcription elongation. A preferred site is one that is critical for the
transcriptional
activity of bacterial RNA polymerase, but one that is not required by the
corresponding
15 mammalian enzyme.
Towards this end, generally the tighter the fit, the lower the steric
hindrances, and
the greater the attractive forces, the more potent the potential modulator
(e.g., an inhibitor)
since these properties are consistent with a tighter'binding constant.
Furthermore, the more
specificity in the design of a potential drug the more likely that the drug
will not interact as
a o well with other proteins. This will minimize potential side-effects due to
unwanted
interactions with other proteins.
Initially alternative compounds known to bind bacterial RNA polymerase,
including
rifampicin analogs, can be systematically modified by computer modeling
programs until
one or more promising potential analogs are identified. In addition systematic
modification
a s of selected analogs can then be systematically modified by computer
modeling programs
until one or more potential analogs are identified. Such analysis has been
shown to be
effective in the development of HIV protease inhibitors [Lam et al., Scietace
263:380-384
(1994); Wlodawer et al., Alan. Rev. Bioclaerrt. 62:543-585 (1993); Appelt,
Perspectives itt
Drug Discovery aftd Design 1:23-48 (1993); Erickson, Perspectives ira Drug
Discovery ajtd
3 o Design 1:109-128 (1993)]. Alternatively a potential modulator could be
obtained by
initially screening a random peptide library produced by recombinant
bacteriophage for
example, [Scott and Smith, Science, 249:386-390 (1990); Cwirla et al., Pt-oc.
Natl. Acad.
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Sci., 87:6378-6382 (1990); Devlin et al., Science, 249:404-406 (1990)]. A
peptide selected
in this manner would then be systematically modified by computer modeling
programs as
described above, and then treated analogously to a structural analog as
described below.
Once a potential modulator/inhibitor is identified it can be either selected
from a
s library of chemicals as are commercially available from most large chemical
companies
including Merck, GlaxoWelcome, Bristol Meyers Squib, Monsanto/Searle, Eli
Lilly,
Novartis and Pharmacia LTpJohn, or alternatively the potential modulator may
be
synthesized de novo. The de novo synthesis of one or even a relatively small
group of
specific compounds is reasonable in the art of drug design. The potential
modulator can be
i o placed into a standard binding assay with RNA polymerase or an active
fragment thereof,
for example. The subunit fragments can be synthesized by either standard
peptide synthesis
described above, or generated through recombinant DNA technology or classical
proteolysis. Alternatively the corresponding full-length proteins may be used
in these
assays.
15 For example, the (3 subunit can be attached to a solid support. Methods for
placing
the [3 subunit on the solid support are well known in the art and include such
things as
linking biotin to the (i subunit and linking avidin to the solid support. The
solid support can
be washed to remove unreacted species. A solution of a labeled potential
modulator (e.g.,
an inhibitor) can be contacted with the solid support. The solid support is
washed again to
a o remove the potential modulator not bound to the support. The amount of
labeled potential
modulator remaining with the solid support and thereby bound to the (3 subunit
can be
determined. Alternatively, or in addition, the dissociation constant between
the labeled
potential modulator and the ~3 subunit, for example can be determined.
Suitable labels for
either the bacterial RNA polymerase subunit or the potential modulator are
exemplified
a s herein. In a particular embodiment, isothermal calorimetry can be used to
determine the
stability of the bacterial RNA polymerase in the absence and presence of the
potential
modulator.
In another embodiment, a Biacore machine can be used to determine the binding
constant of the bacterial RNA polymerase to a DNA template in the presence and
absence
s o of the potential modulator. Alternatively, one or more of the bacterial
RNA polymerase
subunits can be immobilized on a sensor chip. The remaining subunits can then
be
contacted with (e.g., flowed over) the sensor chip to form the bacterial RNA
polymerase.
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In this case the dissociation constant for the bacterial RNA polymerase can be
determined by monitoring changes in the refractive index with respect to time
as buffer is
passed over the chip [O'Shannessy et al. Anal. Biochem. 212:457-468 (1993);
Schuster et
al., Natune 365:343-347 (1993)]. Scatchard plots, for example, can be used in
the analysis
s of the response functions using different concentrations of a particular
subunit. Flowing a
potential modulator at various concentrations over the bacterial RNA
polymerase and
monitoring the response function (e.g., the change in the refractive index
with respect to
time) allows the bacterial RNA polymerase dissociation constant to be
determined in the
presence of the potential modulator and thereby indicates whether the
potential modulator is
i o either an inhibitor, or an agonist of the bacterial RNA polymerase
complex.
In another aspect of the present invention a potential modulator is assayed
for its
ability to inhibit the bacterial RNA polymerase. A modulator that inhibits the
RNA
polymerase can then be selected. In a particular embodiment, the effect of a
potential
modulator on the catalytic and/or transcriptional activity of bacterial RNA
polymerase is
i5 determined. The potential modulator is then be added to a bacterial culture
to ascertain its
effect on bacterial proliferation. A potential modulator that inhibits
bacterial proliferation
can then be selected.
In a particular embodiment, the effect of the potential modulator on the
catalytic
and/or transcriptional activity of the bacterial RNA polymerase is determined
(either
2 o independently, or subsequent to a binding assay as exemplified above). In
one such
embodiment, the rate of the DNA-dependent RNA transcription is determined. For
such
assays a labeled nucleotide could be used. This assay can be performed using a
real-time
assay e.g., with a fluorescent analog of a nucleotide. Alternatively, the
determination can
include the withdrawal of aliquots from the incubation mixture at defined
intervals and
2 5 subsequent placing of the aliquots on nitrocellulose paper or on gels. In
a particular
embodiment the potential modulator is selected when it is an inhibitor of the
bacterial RNA
polymerase.
One assay for RNA polymerase activity is a modification of the method of
Burgess
et al. [J. Biol. Claefn., 244:6160 (1969)]
3 0 [See also http:l/www.worthington-biochem.com/manual/R/RNAP.html].
One unit incorporates one nanomole of UMP into acid insoluble products in 10
minutes at 37°C under the assay conditions such as those listed below.
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The suggested reagents are:
(a) 0.04 M Tris-HCI, pH 7.9, containing 0.01 M MgCl2 , 0.15 M KCl, and 0.5
mg/ml BSA;
(b) Nucleoside triphosphates (NTP) : 0.15 mM each of ATP, CTP, GTP, UTP;
s spiked with 3H - UTP 75000 - 150000 cpms/0.1 ml;
(c) 0.15 mg/ml calf thymus DNA;
(d) 10% cold perchloric acid; and
(e) 1 % cold perchloric acid.
i o 0.1 - 0.5 units of RNA polymerase in 5 ~.1- 10 w1 is used as the starting
enzyme
concentration.
The procedure is to add 0.1 ml Tris-HCI, 0.1 ml NTP and 0.1 ml DNA to a test
tube
for each sample or blank. At zero time enzyme (or buffer for blank) is added
to each test
tube, and the contents are then mixed and incubated at 37 C for 10 minutes. 1
ml of 10%
15 perchloric acid is added to the tubes to stop the reaction. The acid
insoluble products can
be collected by vacuum filtration through MILLIPORE filter discs having a pore
size of
0.45 a - 10 a (or equivalent). The filters are then washed four times with 1%
cold
perchloric acid using 1 ml - 3 ml for each wash. These filters are then placed
in scintillation
vials. 2 mls of methyl cellosolve are added to the scintillation vials to
dissolve the filters.
a o When the filters are completely dissolved (after about five minutes) 10
mls of scintillation
fluid are added and the vials are counted in a scintillation counter.
For calculation of units of RNA polymerase/mg of protein the following
equation
can be used:
units/mg - CPMtesr - CPM;,;ank
2s CPMtotal ~ mg protein;" test
Alternative transcription assays can also be employed [see Example below, and
Nudler et al., Science 265:793-796 (1994)]. One such assay comprises a core
RNAP that
can be incubated with a suitable 6 subunit to form the holoenzyme. A potential
modulator
can then be added prior to, simultaneously with, subsequently to a promoter
fragment (e.g.,
s o T7A1 as exemplified below). RNA synthesis is then initiated by the
addition of a primer
(e.g., a CpA primer) and the four nucleotide triphosphates (NTPs). The RNA
synthesis in
the presence and absence of the potential modulator is then quantified. In the
Example
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below, a radioactive nucleotide was employed and the radioactive RNA products
were
analyzed on a 15% polyacrylamide sequencing gel. Alternatively, a fluorescent
nucleotide
analog can be used. Transcription reactions on a minimal scaffold system can
be performed
as shown in Fig. 6b below in the presence and the absence of the potential
modulator [see
s also Korzheva et al., Science 2$9:619-625 (2000)].
When suitable potential modulators are identified, a supplemental crystal can
be
prepared which comprises the bacterial RNA polymerase and the potential
modulator (see
Example below). Preferably the crystal effectively diffracts X-rays for the
determination of
the atomic coordinates of the protein-ligand complex to a resolution of better
than 5.0
1 o Angstroms, more preferably equal to or better than 3.5 Angstroms. The
three-dimensional
structure of the supplemental crystal can be determined by Molecular
Replacement
Analysis. Molecular replacement involves using a known three-dimensional
structure as a
search model to determine the structure of a closely related molecule or
protein-ligand
complex in a new crystal form. The measured X-ray diffraction properties of
the new
i5 crystal are compared with the search model structure to compute the
position and
orientation of the protein in the new crystal. Computer programs that can be
used include:
X-PLOR (see above), CNS, (Crystallography and NMR System, a next level of
XPLOR),
and AMORE [J. Navaza, Acta Crystallog~aphics ASO, 157-163 (1994)]. Once the
position
and orientation are known an electron density map can be calculated using the
search model
a o to provide X-ray phases. Thereafter, the electron density is inspected for
structural
differences and the search model is modified to conform to the new structure.
Using this
approach, it is also possible to use the claimed crystal of the Rif RNAP
complex to solve
the three-dimensional structures of other bacterial core RNA polymerases bound
to
rifampicin (and/or other inhibitors) having pre-ascertained amino acid
sequences. Other
~ s computer programs that can be used to solve the structures of the
bacterial RNA polymerase
from other organisms include: QUANTA, CHARMM; INSIGHT; SYBYL;
MACROMODE; and ICM.
A candidate drug can be selected by performing rational drug design with the
three-dimensional structure determined for the supplemental crystal,
preferably in
3 o conjunction with computer modeling discussed above (see below). The
candidate drug
(e.g., a potential modulator of bacterial RNA polymerase) can then be assayed
as
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exemplified above, or ifs situ. A candidate drug can be identified as a drug,
for example, if
it inhibits bacterial proliferation.
A potential inhibitor (e.g., a candidate drug) would be expected to interfere
with
bacterial growth. Therefore, an assay that can measure bacterial growth may be
used to
s identify a candidate drug.
Methods of testing a potential bactericidal agent (e.g., the candidate drug)
in an
animal model are well known in the art, and can include standard bactericidal
assays. The
potential modulators can be administered by a variety of ways including
topically, orally,
subcutaneously, or intraperitoneally depending on the proposed use. Generally,
at least two
1 o groups of animals are used in the assay, with at least one group being a
control group which
is administered the administration vehicle without the potential modulator.
For all of the drug screening assays described herein further refinements to
the
structure of the drug will generally be necessary and can be made by the
successive
iterations of any and/or all of the steps provided by the particular drug
screening assay.
15 Rational Drug Design
Computational techniques can be used to screen, identify, select and design
chemical entities capable of associating with Taq RNAP or subunit thereof or
structurally
homologous molecules. IW owledge of the structure coordinates for Taq RNAP
permits the
design and/or identification of synthetic compounds and/or other molecules
which have a
a o shape complementary to the conformation of the Taq RNAP binding site. In
particular,
computational techniques can be used to identify or design chemical entities,
such as
inhibitors, agonists and antagonists, that associate with a Taq RNAP binding
pocket or a
Taq RNAP-like binding pocket. Inhibitors may bind to or interfere with all or
a portion of
the binding pocket of Taq RNAP, and can be competitive, non-competitive, or
a s uncompetitive inhibitors; or interfere with dimerization by binding at the
interface between
the two monomers. Once identified and screened for biological activity, these
inhibitors/agonists/antagonists may be used therapeutically or
prophylactically to block
Taq RNAP activity and, thus, result in inhibition of growth or death of the
bacteria.
Structure-activity data for analogs of ligands that bind to or interfere with
Taq RNAP or
s o Taq RNAP binding pockets can also be obtained computationally.
The term "chemical entity," as used herein, refers to agents, complexes of two
or
more agents, and fragments of such agents or complexes. Chemical entities that
are
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determined to associate with Taq RNAP are potential drug candidates. Data
stored in a
machine-readable storage medium that is capable of displaying a graphical
three-
dimensional representation of the structure of Taq RNAP or a structurally
homologous
molecule, as identified herein, or portions thereof may thus be advantageously
used for drug
discovery. The structure coordinates of the chemical entity are used to
generate a three-
dimensional image that can be computationally fit to the three-dimensional
image of Taq
RNAP or a structurally homologous molecule. The three-dimensional molecular
structure
encoded by the data in the data storage medium can then be computationally
evaluated for
its ability to associate with chemical entities. When the molecular structures
encoded by the
Z o data is displayed in a graphical three-dimensional representation on a
computer screen, the
protein structure can also be visually inspected for potential association
with chemical
entities.
One embodiment of the method of drug design involves evaluating the potential
association of a known chemical entity with Taq RNAP or a structurally
homologous
i5 molecule, particularly with a Taq RNAP binding pocket or Taq RNAP-like
binding pocket.
The method of drug design thus includes computationally evaluating the
potential of a
selected chemical entity to associate with any of the molecules or molecular
complexes set
forth above. This method comprises the steps of: (a) employing computational
means to
perform a fitting operation between the selected chemical entity and a binding
pocket of the
a o molecule or molecular complex; and (b) analyzing the results of said
fitting operation to
quantify the association between the chemical entity and the binding pocket.
In another embodiment, the method of drug design involves computer assisted
design of chemical entities that associate with Taq RNAP, its homologs, or
portions thereof.
Chemical entities can be designed in a step-wise fashion, one fragment at a
time, or may be
25 designed as a whole or "de novo." To be a viable drug candidate, the
chemical entity
identified or designed according to the method must be capable of structurally
associating
with at least part of a Taq RNAP or Taq RNAP-like binding pockets, and must be
able,
sterically and energetically, to assume a conformation that allows it to
associate with the
Taq RNAP or Taq RNAP-like binding pocket. Non-covalent molecular interactions
3 o important in this association include hydrogen bonding, van der Waals
interactions,
hydrophobic interactions, and electrostatic interactions. Conformational
considerations
include the overall three-dimensional structure and orientation of the
chemical entity in
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relation to the binding pocket, and the spacing between various functional
groups of an
entity that directly interact with the Taq RNAP-like binding pocket or
homologs thereof.
Optionally, the potential binding of a chemical entity to a Taq RNAP or Taq
RNAP-like binding pocket is analyzed using computer modeling techniques prior
to the
s actual synthesis and testing of the chemical entity. If these computational
experiments
suggest insufficient interaction and association between it and the Taq RNAP
or Taq
RNAP-like binding pocket, testing of the entity is obviated. However, if
computer modeling
indicates a strong interaction, the molecule may then be synthesized and
tested for its ability
to bind to or interfere with a Taq RNAP or Taq RNAP binding pocket. Binding
assays to
s o determine if a compound actually binds to Taq RNAP can also be performed
and are well
known in the art. Binding assays may employ kinetic or thermodynamic
methodology using
a wide variety of techniques including, but not limited to, microcalorimetry,
circular
dichroism, capillary zone electrophoresis, nuclear magnetic resonance
spectroscopy,
fluorescence spectroscopy, and combinations thereof.
15 One skilled in the art rnay use one of several methods to screen chemical
entities
or fragments for their ability to associate with a Taq RNAP or Taq RNAP
binding pocket.
This process may begin by visual inspection of, for example, a Taq RNAP or Taq
RNAP-
like binding pocket on the computer screen based on the Taq RNAP structure
coordinates in
Table 2 or other coordinates which define a similar shape generated from the
machine-
a o readable storage medium. Selected fragments or chemical entities may then
be positioned in
a variety of orientations, or docked, within the binding pocket. Docking may
be
accomplished using software such as QUANTA and SYBYL, followed by energy
minimization and molecular dynamics with standard molecular mechanics
forcefields,
such as CHARMM and AMBER.
2 s Specialized computer programs may also assist in the process of selecting
fragments or chemical entities. Examples include GRID (P.J. Goodford, J. Med.
Chem.
28:849-857 (1985); available from Oxford University, Oxford, UK); MCSS (A.
Miranker et
al., Proteins: Struct. Funct. Genj 1:29-34 (1991); available from Molecular
Simulations,
San Diego, CA); AUTODOCK (D.S. Goodsell et al., Proteins: Struct. Funct.
Genet. 8:195-
3 0 202 (1990); available from Scripps Research Institute, La Jolla, CA); and
DOCK (LD.
Kuntz et al., J. Mol. Biol. 161:269-288 (1982); available from University of
California, San
Francisco, CA).
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Once suitable chemical entities or fragments have been selected, they can be
assembled into a single compound or complex. Assembly may be preceded by
visual
inspection of the relationship of the fragments to each other on the three
dimensional image
displayed on a computer screen in relation to the structure coordinates of Taq
RNAP. This
s would be followed by manual model building using software such as QUANTA or
SYBYL (Tripos Associates, St. Louis, MO).
Useful programs to aid one of skill in the art in connecting the individual
chemical
entities or fragments include, without limitation, CAVEAT (P.A. Bartlett et
al., in
Molecular Recognition in Chemical and Biological Problems," Special Publ.,
Royal Chem.
to Soc., 78:182-196 (1989); G. Lauri et al., J. Comput. Aided Mol. Des. 8:51-
66 (1994);
available from the University of California, Berkeley, CA); 3D database
systems such as
ISIS (available from MDL Information Systems, San Leandro, CA; reviewed in
Y.C.
Martin, J. Med. Chem. 35:2145-2154 (1992)); and HOOK (M.B. Eisen et al.,
Proteins:
Struc., Funct., Genet. 19:199-221 (1994); available from Molecular
Simulations, San
i5 Diego, CA).
Taq RNAP binding compounds may be designed "de novo" using either an empty
binding site or optionally including some portions) of a known inhibitor(s).
There are many
de novo ligand design methods including, without limitation, LUDI (H.-J. Bohm,
J. CoMp.
Aid. Molec. Design. 6:61-78 (1992); available from Molecular Simulations Inc.,
San Diego,
2o CA); LEGEND (Y. Nishibata et al., Tetrahedron, 47:8985 (1991); available
from Molecular
Simulations Inc., San Diego, CA); LeapFrog (available from Tripos Associates,
St. Louis,
MO); and SPROUT (V. Gillet et al., J. Cpmput. Aided Mol. Desi 7:127-153
(1993);
available from the University of Leeds, UK).
Once a compound has been designed or selected by the above methods, the
2 s efficiency with which that entity may bind to or interfere with a Taq RNAP
or Taq RNAP-
like binding pocket may be tested and optimized by computational evaluation.
For example,
an effective Taq RNAP or Taq RNAP-like binding pocket inhibitor must
preferably
demonstrate a relatively small difference in energy between its bound and free
states (i.e., a
small deformation energy of binding). Thus, the most efficient Taq RNAP or Taq
RNAP-
3 0 like binding pocket inhibitors should preferably be designed with a
deformation energy of
binding of not greater than about 10 kcal/mole; more preferably, not greater
than 7
kcal/mole. Taq RNAP or Taq RNAP-like binding pocket inhibitors may interact
with the
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binding pocket in more than one conformation that is similar in overall
binding energy. In
those cases, the deformation energy of binding is taken to be the difference
between the
energy of the free entity and the average energy of the conformations observed
when the
inhibitor binds to the protein.
s An entity designed or selected as binding to or interfering with a Taq RNAP
or
Taq RNAP-like binding pocket may be further computationally optimized so that
in its
bound state it would preferably lack repulsive electrostatic interaction with
the target
enzyme and with the surrounding water molecules. Such non-complementary
electrostatic
interactions include repulsive charge-charge, dipole-dipole, and charge-dipole
interactions.
s o Specific computer software is available in the art to evaluate compound
deformation energy and electrostatic interactions. Examples of programs
designed for such
uses include: Gaussian 94, revision C (M.J. Frisch, Gaussian, Inc.,
Pittsburgh, PA (1995));
AMBER, version 4.1 (P.A. I~ollman, University of California at San Francisco,
(1995));
QUANTA/CHARl 4M (Molecular Simulations, Inc., San Diego, CA (1995)); Insight
15 II/Discover (Molecular Simulations, Inc., San Diego, CA (1995)); Delphi
(Molecular
Simulations, Inc., San Diego, CA (1995)); and AMSOL (Quantum Chemistry Program
Exchange, Indiana University). These programs may be implemented, for
instance, using a
Silicon Graphics workstation such as an Indigo2 with "MPACT" graphics. Other
hardware
systems and software packages will be known to those skilled in the art.
a o Another approach encompassed by this invention is the computational
screening
of databases for small molecules, chemical entities, compounds or other
modulators that
can bind in whole, or in part, to a Taq RNAP or Taq RNAP-like binding pocket.
In this
screening, the quality of fit of such entities to the binding site may be
judged either by shape
complementarity or by estimated interaction energy (E.C. Meng et al., J. Comp.
Chem., 13,
25 pp. 505-524 (1992)).
This invention also enables the development of chemical entities that can
isomerize to short-lived reaction intermediates in the chemical reaction of a
substrate or
other compound that binds to or with Taq RNAP. Time-dependent analysis of
structural
changes in Taq RNAP during its interaction with other molecules is carried
out. The
3 o reaction intermediates of Taq RNAP can also be deduced from the reaction
product in co-
complex with Taq RNAP. Such information is useful to design improved analogs
of known
Taq RNAP inhibitors or to design novel classes of inhibitors based on the
reaction
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intermediates of the Taq RNAP and inhibitor co-complex. This provides a novel
route for
designing RNAP inhibitors with both high specificity and stability.
Yet another approach to rational drug design involves probing the Taq RNAP
crystal of the invention with molecules comprising a variety of different
functional groups
s to determine optimal sites for interaction between candidate Taq RNAP
inhibitors and the
protein. For example, high resolution x-ray diffraction data collected from
crystals soaked
in or co-crystallized with other molecules allows the determination of where
each type of
solvent molecule sticks. Molecules that bind tightly to those sites can then
be further
modified and synthesized and tested for their hepes protease inhibitor
activity (J. Travis,
so Science, 262:1374 (1993)).
In a related approach, iterative drug design is used to identify inhibitors of
Taq
RNAP. Iterative drug design is a method for optimizing associations between a
protein and
a compound by determining and evaluating the three dimensional structures of
successive
sets of protein/compound complexes. In iterative drug design, crystals of a
series of
15 protein/compound complexes are obtained and then the three-dimensional
structures of
each complex is solved. Such an approach provides insight into the association
between the
proteins and compounds of each complex. This is accomplished by selecting
compounds
with inhibitory activity, obtaining crystals of this new protein/compound
complex, solving
the three-dimensional structure of the complex, and comparing the associations
between the
2 o new protein/compound complex and previously solved protein/compound
complexes. By
observing how changes in the compound affected the protein/compound
associations, these
associations may be optimized.
Labels
Suitable labels include enzymes, fluorophores e.g., fluorescein isothiocyanate
25 (FITC), phycoerythrin (PE), Texas red (TR), rhodamine, free or chelated
lanthanide series
salts, especially Eu3+, to name a few fluorophores and including fluorescent
GTP and GDP
analogs such as mantGTP and mantGDP, chromophores, radioisotopes, chelating
agents,
dyes, colloidal gold, latex particles, ligands (e.g., biotin), and
chemiluminescent agents.
When a control marker is employed, the same or different labels may be used
for the test
3 o and control marker.
In the instance where a radioactive label, such as the isotopes 3H, 14C, 32P,
3sS~ ssCh
slCr, s~Co, sBCo, s9Fe, 9°Y, lash 1311, and 186Re are used, known
currently available counting
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procedures may be utilized. In the instance where the label is an enzyme,
detection may be
accomplished by any of the presently utilized colorimetric,
spectrophotometric,
fluorospectrophotometric, amperometric or gasometric techniques known in the
art.
Direct labels are one example of labels which can be used according to the
present
s invention. A direct label has been defined as an entity, which in its
natural state, is readily
visible, either to the naked eye, or with the aid of an optical filter and/or
applied
stimulation, e.g. ultraviolet light to promote fluorescence. Among examples of
colored
labels, which can be used according to the present invention, include metallic
sol particles,
for example, gold sol particles such as those described by Leuvering (U.S.
Patent
io 4,313,734); dye sole particles such as described by Gribnau et al. (U.S.
Patent 4,373,932 )
and May et al. (WO 88/08534); dyed latex such as described by May, supra,
Snyder (EP-A
0 280 559 and 0 281 327); or dyes encapsulated in liposomes as described by
Campbell et
al. (U.S. Patent 4,703,017) Other direct labels include a radionucleotide, a
luminescent
moiety, or a fluorescent moiety including as a modified/fusion chimera of
green fluorescent
15 protein (as described in U.S. Patent No. 5,625,048 filed April 29, 1997,
and WO 97/26333,
published July 24, 1997). In addition to these direct labeling devices,
indirect labels
comprising enzymes can also be used according to the present invention.
Various types of
enzyme linked immunoassays are well known in the art, for example, alkaline
phosphatase
and horseradish peroxidase, lysozyme, glucose-6-phosphate dehydrogenase,
lactate
a o dehydrogenase, urease, these and others have been discussed in detail by
Eva Engvall in
Enzyme Immunoassay ELISA and EMIT in Methods ih Efazymology, 70:419-439 (1980)
and in U.S. Patent 4,857,453.
Suitable enzymes include, but are not limited to, alkaline phosphatase and
horseradish peroxidase. Other labels for use in the invention include magnetic
beads or
a s magnetic resonance imaging labels.
In another embodiment, a phosphorylation site can be created on an antibody of
the
invention for labeling with 32P, e.g., as described in European Patent No.
0372707
(application No. 89311108.8) by Sidney Pestka, or U.S. Patent No. 5,459,240,
issued
October 17, 1995 to Foxwell et al.
3 o As exemplified herein, proteins, including antibodies, can be labeled by
metabolic
labeling. Metabolic labeling occurs during ira vitro incubation of the cells
that express the
protein in the presence of culture medium supplemented with a metabolic label,
such as
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[3sS]_methionine or [3zP]-orthophosphate. In addition to metabolic (or
biosynthetic)
labeling with [3sS]-methionine, the invention further contemplates labeling
with [14C]-
amino acids and [3H]-amino acids (with the tritium substituted at non-labile
positions)
Three-Dimensional Representation of the Structure of the Rif RNAP complex
s In addition, the present invention provides a computer that comprises a
representation of the RNAP-RNAP binding partner complex (e.g., the Rif RNAP
complex)
in computer memory that can be used to screen for compounds that will or are
likely to
inhibit RNAP. In a related embodiment, the computer can be used in the design
of altered
RNAPs that have either enhanced, or alternatively diminished RNA polymerase
activity.
i o Preferably, the computer comprises portions of and/or all of the
information contained in
Table 2. In a particular embodiment, the computer comprises: (i) a machine-
readable data
storage material encoded with machine-readable data, (ii) a working memory for
storing
instructions for processing the machine readable data, (iii) a central
processing unit coupled
to the working memory and the machine-readable data storage material for
processing the
i5 machine-readable data into a three-dimensional representation, and (iv) a
display coupled to
the central processing unit for displaying the three-dimensional
representation.
Thus the machine-readable data storage medium comprises a data storage
material
encoded with machine readable data which can comprise portions and/or all of
the
structural information contained in Table 2. One embodiment for manipulating
and
z o displaying the structural data provided by the present invention is
schematically depicted in
Figure 7. As depicted, the System 1, includes a computer 2 comprising a
central processing
unit ("CPU") 3, a working memory 4 which may be random-access memory or "core"
memory, mass storage memory 5 (e.g., one or more disk or CD-ROM drives), a
display
terminal 6 (e.g., a cathode-ray tube), one or more keyboards 7, one or more
input lines 10,
z 5 and one or more output lines 20, all of which are interconnected by a
conventional
bidirectional system bus 30.
Input hardware 12, coupled to the computer 2 by input lines 10, may be
implemented in a variety of ways. Machine-readable data may be inputted via
the use of
one or more modems 14 connected by a telephone line or dedicated data line 16.
3 o Alternatively or additionally, the input hardware 12 may comprise CD-ROM
or disk drives
5. In conjunction with the display terminal 6, the keyboard 7 may also be used
as an input
device. Output hardware 22, coupled to computer 2 by output lines 20, may
similarly be
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implemented by conventional devices. Output hardware 22 may include a display
terminal
6 for displaying the three dimensional data. Output hardware might also
include a printer
24, so that a hard copy output may be produced, or a disk drive 5, to store
system output for
later use, see also U.S. Patent No: 5,978,740, Issued November 2, 1999.
s In operation, the CPU 3 (i) coordinates the use of the various input and
output
devices 12 and 22; (ii) coordinates data accesses from mass storage 5 and
accesses to and
from working memory 4; and (iii) determines the sequence of data processing
steps. Any of
a number of programs may be used to process the machine-readable data of this
invention.
The invention thus further provides a machine-readable storage medium
io comprising a data storage material encoded with machine readable data
which, when using
a machine programmed with instructions for using said data, is capable of
displaying a
graphical three-dimensional representation of any of the molecule or molecular
complexes
of this invention that have been described above. In a preferred embodiment,
the machine-
readable data storage medium comprises a data storage material encoded with
machine
i5 readable data which, when using a machine programmed with instructions for
using said
data, is capable of displaying a graphical three-dimensional representation of
a molecule or
molecular complex comprising all or any parts of an RNAP binding pocket or an
RNAP-
like binding pocket, as defined above. In another preferred embodiment, the
machine-
readable data storage medium is capable of displaying a graphical three-
dimensional
z o representation of all the amino acids of a molecule or molecular complex
defined by the
structure coordinates in Table 2 +/- a root mean square deviation from the
backbone atoms
of said amino acids of not more than 1.1~.
In an alternative embodiment, the machine-readable data storage medium
comprises a data storage material encoded with a first set of machine readable
data which
z 5 comprises the Fourier transform of the structure coordinates set forth in
Table 2, and which,
when using a machine programmed with instructions for using said data, can be
combined
with a second set of machine readable data comprising the x-ray diffraction
pattern of a
molecule or molecular complex to determine at least a portion of the structure
coordinates
corresponding to the second set of machine readable data.
3 o For example, a system for reading a data storage medium may include a
computer
comprising a central processing unit ("CPU"), a working memory which may be,
e.g., RAM
(random access memory) or "core" memory, mass storage memory (such as one or
more
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disk drives or CD-ROM drives), one or more display devices (e.g., cathode-ray
tube
("CRT") displays, light emitting diode ("LED") displays, liquid crystal
displays ("LCDs"),
electroluminescent displays, vacuum fluorescent displays, field emission
displays ("FEDs"),
plasma displays, projection panels, etc.), one or more user input devices
(e.g., keyboards,
s microphones, mice, touch screens, etc.), one or more input lines, and one or
more output
lines, all of which are interconnected by a conventional bidirectional system
bus. The
system may be a stand-alone computer, or may be networked (e.g., through local
area
networks, wide area networks, intranets, extranets, or the Internet) to other
systems (e.g.,
computers, hosts, servers, etc.). The system may also include additional
computer
s o controlled devices such as consumer electronics and appliances.
Input hardware may be coupled to the computer by input lines and may be
implemented in a variety of ways. Machine-readable data of this invention may
be inputted
via the use of a modem or modems connected by a telephone line or dedicated
data line.
Alternatively or additionally, the input hardware may comprise CD-ROM drives
or disk
i5 drives. In conjunction with a display terminal, a keyboard may also be used
as an input
device.
Output hardware may be coupled to the computer by output lines and may
similarly be implemented by conventional devices. By way of example, the
output hardware
may include a display device for displaying a graphical representation of a
binding pocket
z o of this invention using a program such as QUANTA as described herein.
Output hardware
might also include a printer, so that hard copy output may be produced, or a
disk drive, to
store system output for later use.
In operation, a CPU coordinates the use of the various input and output
devices,
coordinates data accesses from mass storage devices, accesses to and from
working
z s memory, and determines the sequence of data processing steps. A number of
programs may
be used to process the machine-readable data of this invention. Such programs
are
discussed in reference to the computational methods of drug discovery as
described herein.
References to components of the hardware system are included as appropriate
throughout
the following description of the data storage medium.
3 o Machine-readable storage devices useful in the present invention include,
but are not
limited to, magnetic devices, electrical devices, optical devices, and
combinations thereof.
Examples of such data storage devices include, but are not limited to, hard
disk devices, CD
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devices, digital video disk devices, floppy disk devices, removable hard disk
devices,
magneto-optic disk devices, magnetic tape devices, flash memory devices,
bubble memory
devices, holographic storage devices, and any other mass storage peripheral
device. It
should be understood that these storage devices include necessary hardware
(e.g., drives,
s controllers, power supplies, ect.) as well as any necessary media (e.g.,
disks, flash cards,
etc.) to enable the storage data.
Pharmaceutical Compositions
Pharmaceutical compositions of this invention comprise an inhibitor of Taq
RNAP activity identified according to the invention, or a pharmaceutically
acceptable salt
1 o thereof, and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
The term "pharmaceutically acceptable carrier" refers to a carriers) that is
"acceptable" in the sense of being compatible with the other ingredients of a
composition
and not deleterious to the recipient thereof. Optionally, the pH of the
formulation is
adjusted with pharmaceutically acceptable acids, bases, or buffers to enhance
the stability
i5 of the formulated compound or its delivery form.
Methods of making and using such pharmaceutical compositions are also included
in the invention. The pharmaceutical compositions of the invention can be
administered
orally, parenterally, by inhalation spray, topically, rectally, nasally,
buccally, vaginally, or
via an implanted reservoir. Oral administration or administration by injection
is preferred.
z o The term parenteral as used herein includes subcutaneous, intracutaneous,
intravenous,
intramuscular, infra-articular, intrasynovial, intrasternal, intrathecal,
intralesional, and
intracranial injection or infusion techniques.
Dosage levels of between about 0.01 and about 100 mg/kg body weight per day,
preferably between about 0.5 and about 75 mg/kg body weight per day of the Taq
RNAP
a s inhibitory compounds described herein are useful for the prevention and
treatment of Taq
RNAP mediated disease. Typically, the pharmaceutical compositions of this
invention will
be administered from about 1 to about 5 times per day or alternatively, as a
continuous
infusion. Such administration can be used as a chronic or acute therapy. The
amount of
active ingredient that may be combined with the carrier materials to produce a
single dosage
s o form will vary depending upon the host treated and the particular mode of
administration. A
typical preparation will contain from about 5% to about 95% active compound
(w!w).
Preferably, such preparations contain from about 20% to about 80% active
compound.
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The present invention may be better understood by reference to the following
non-
limiting Example, which is provided as exemplary of the invention. The
following example
is presented in order to more fully illustrate the preferred embodiments of
the invention. It
should in no way be construed, however, as limiting the broad scope of the
invention.
s EXAMPLES
STRUCTURAL MECHANISM FOR RIFAMPICIN INHIBITION
OF BACTERIAL RNA POLYMERASE
Introduction
s o High-resolution structural studies of the Rif RNAP complex should lead to
insights
into rifampicin binding, the mechanism of inhibition, and also the mechanism
by which
mutations lead to Rife. These structural studies will also shed light on the
transcription
mechanism itself, as well as provide the basis for the development of drugs
that selectively
inhibit bacterial RNAPs, but are less prone than rifampicin to lead bacterial
15 mutations/substitutions of single amino acids that give rise to resistance.
Indeed, the recent
determination of the crystal structure of core RNAP from Thermus aquaticus
(Taq) [Zhang
et al., Cell 98:811-824 (1999)] has opened the door to further studies of RNAP
structure,
function, and interactions with substrates, ligands, and inhibitors.
To further provide a more detailed framework to interpret the existing
genetic,
a o biochemical, and biophysical information, as well as to guide further
studies aimed at
understanding the transcription process and its regulation, the three-
dimensional structure of
a bacterial core RNAP complexed with rifampicin was determined by X-ray
crystallography
at 3.3 t~ resolution as detailed below. The structure explains the effects of
rifampicin on
RNAP function. In combination with a model of the ternary transcription
complex and
25 biochemical experiments, the data indicate that the predominant effect of
rifampicin on
RNAP function is to directly block the path of the elongating RNA transcript
at the 5'-end
when the transcript becomes either 2 or 3 nucleotides in length.
Methods
Purification. grad crystalliaatiora: Native Taq core RNAP was purified and
s o crystallized as described previously [Zhang et al., Cell 98:811-824
(1999)]. Crystals were
subsequently soaked in stabilization solution [2 M (NH4)ZS04, 0.1 M Tris-HCI,
pH 8.0, and
20 mM MgCl2] with 0.1 mM rifampicin for at least 12 hours. The crystals were
then
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prepared for cryo-crystallography by soaking in stabilization solution
containing 50% (w/v)
sucrose for 30 minutes before flash freezing in liquid nitrogen. Diffraction
data was
collected at the APS beamline SBC 19ID using 0.3 oscillations, and processed
using
DENZO and SCALEPACK [Otwinowski, Isomorphous Replacement and Ayaomalous
s Scattef~ing (eds. Wolf, Evans and Leslie) Science and Engineering Research
Council,
Daresbury Laboratory, Daresbury, UK, (1991)].
In short, the preparative procedure for T. aquaticus core RNAP is similar to
the
preparation of E. coli core RNAP [Polyakov et al., Cell, 83:365-373 (1995)].
Briefly,
approximately 200 g wet cell paste is thawed and lysed using a continuous-flow
French
s o press. After a low-speed spin, the soluble fraction is precipitated with
0.6% Polymin-P.
RNAP is eluted from the Polymin-P pellet with TGED buffer (10 mM Tris HCI, pH
8,
5% glycerol, 1 mM EDTA, 1 mM DTT) plus 1 M NaCI, then precipitated by adding
33%(g/v) ammonium sulfate. The pellet is resuspended and loaded onto a 50 ml
column of
heparin-SEPHAROSE FF (Pharmacia) equilibrated with TGED buffer plus 0.2 M
NaCI.
s5 The RNAP is eluted from the column with TGED buffer plus 0.6 M NaCI. The
RNAP was
again precipitated with ammonium sulfate, then resuspended and loaded on a
SUPERDEX-200 gel filtration column equilibrated with TGED buffer plus 0.5 M
NaCI.
Fractions containing RNAP were pooled and loaded onto a MONO-Q (Pharmacia)
ion-exchange column equilibrated with TGED buffer plus 0.1 M NaCI. The protein
was
a o eluted with a gradient from 0.1 to 0.5 M NaCI. The RNAP peak eluted at
around 0.3 M
NaCI. The RNAP was concentrated using a centrifugal filter, then loaded onto
an SP
SEPHAROSE (Pharmacia) column equilibrated in TGED buffer plus 0.1 M NaCI.
After
loading, the column was incubated at 4 C for at least 10 hours, then pure RNAP
was
eluted with a 0.1 to 0.5 M NaCI gradient (core RNAP elutes at around 0.3 M
NaCI). 200 g
a s wet cell paste typically yielded 15 mg of core RNAP, which was more than
99% pure as
judged from overloaded, Coomassie-stained SDS gels. This sample is ready for
crystallization.
Crystals of T. aquaticus core RNAP were grown by vapor diffusion. 10 ~.l of T.
aquaticus core RNAP (17 mg/ml) was mixed with the same volume of a solution
containing
3 0 40-45% saturated (NH4)ZSO4, 0.1 M Tris-HCI, pH 8.0, and 20 mM MgCl2, and
incubated as
a hanging drop over the same solution. Crystals grow in 2-3 weeks to typical
dimensions of
0.15 mm X 0.15 mm X 0.4 mm at room temperature. For cryo-crystallography, the
crystals
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are pre-soaked in stabilization solution (same as the crystallization solution
except with
50% saturated ammonium sulfate). The crystals are then soaked in stabilization
solution
containing 50% (g/v) sucrose for about 30 minutes before flash freezing. The
frozen
crystals diffract to 5.0 ~ from an in-house X-ray generator. Spots can
sometimes be
s observed, in one direction, to 2.7 t~ resolution at synchrotron beamlines.
Diffraction data
was processed using DENZO and SCALEPACK [Otwinowski, Isomorphous Replacement
and Anomalous Scattering (eds. Wolf, Evans and Leslie) Science and Engineering
Research
Council, Daresbury Laboratory, Daresbury, UK, (1991)].
Selenomethionyl core RNAP was prepared and crystallized using the same
io procedures from T, aquaticus cells grown in minimal media (culture medium
162) [Degryse
et al., Arch. Microbiol., 117:189-196 (1978)]. Cells were induced to
incorporate
selenomethionine by suppression of methionine biosynthesis [Doublie, Methods
Erazymol.,
276:523-530 (1997)].
Structure Determination: The native core RNAP structure [Zhang et al., Cell
15 98:811-824 (1999)] was used as a starting model for rigid body refinement
and positional
refinement against the observed amplitudes from the Rif RNAP complex crystal
(Fo 'F:)
using CNS [Adams et al., P~°oc. Natl. Acad. Sci. USA, 94:5018-5023
(1997)], yielding an
initial R-factor of 0.354 (Rfree = 0.41, where the same set of reflections was
set aside as was
used for the Rfree determination of the native structure) for data from 100 -
3.2 A resolution.
a o An initial Fourier difference map, calculated using ~F°Rif -
F°natl amplitude coefficients and
using phases calculated from the native core RNAP structure (cp°at)
clearly revealed density
for the Rif molecule (Fig. 3a). Multiple rounds of manual rebuilding against
(2~F°~ - ~F~~)
maps using O [Jones et al., Acta Cryst, A 47:110-119 (1991)], and refinement
using CNS
[Adams et al., Proc. Natl. Acad. Sci. USA, 94:5018-5023 (1997)] resulted in
the current
2s model (Table 1). At later stages of the refinement, the Rif X-ray crystal
structure [Brufani
et al., J. Molec.Biol. 87:409-435 (1974)] was easily placed into the
difference density.
Included in the model is the recently determined sequence of the Taq co
subunit modeled
earlier as a polyalanine chain [Zhang et al., Cell 98:811-824 (1999)]. Absent
from the
model is a 300 amino acid, non-conserved domain inserted between conserved
regions A
3 o and B of the [f subunit [Zhang et al., Cell 98:811-824 (1999)].
Assays: Taq cells were tested for sensitivity to rifampicin on solid media.
Plates
containing 3% bactoagar and 1l5 dilution of Luria broth were poured with and
without 50
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CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
wg/ml of rifampicin. Cells from frozen stock were then streaked onto plates
and incubated
at 65°C for 2 days and assessed for growth.
The transcription assay comparing rifampicin inhibition of E. coli and Taq
RNAPs
(Fig. 2a) was performed as previously described [Nudler et al., Science
265:793-796
s (1994)]. Briefly, 0.1 pmol of purified Taq core RNAP [Zhang et al., Cell
98:811-824
(1999)] was incubated with Tay aA in 20 ~.1 of transcription buffer (40 mM
Tris-HCI, pH
7.9, 40 mM KCI, 5 mM MgCl2) for 15 minutes at 37°C to form holoenzyme.
Rifampicin
was added to the final concentrations indicated in Fig. 2a and incubated
another 5 minutes
at 37 C, followed by the addition of 0.15 pmol of T7A1 promoter fragment and
incubation
s o for 5 minutes at 37 C. RNA synthesis was initiated by the addition of CpA
primer (100
pM), NTPs (25 p.M each), and a-[32P]UTP (0.3 p.M), and the reaction was
stopped after
incubation for 10 minutes at 37°C. The assay for E.coli RNAP holoenzyme
was the same
except the CpA primer was added to a concentration of 10 ~,M. Radioactive RNA
products
were analyzed on a 15% polyacrylamide sequencing gel.
i5 Assays for extension of the Rif nucleotide compounds (Fig. 2c-2d) were
carried out
as described [Mustaev et al.,Proc.Nat.Acad.Sci. USA 91:12036-12040 (1994)]
with minor
modifications. After binary complex formation, transcription reactions were
started by the
addition 10 wM Rif (CH2)"A compound, with the 'n' indicated in Fig. 2c-2d, and
a-[32P]UTP (0.3 p.M). The reactions were incubated for 2 minutes at room
temperature for
z o E.coli RNAP and 3 minutes at 55 C for Taq. Under these conditions, the
reaction was not
complete, and the yield of the Rif (CH2)n ApU depended on the linker length.
Radioactive
RNA products were analyzed on a 23% polyacrylamide sequencing gel.
Transcription reactions on the minimal scaffold system shown (Fig. 6b) were
performed as described [Korzheva et al., Science 289:619-625 (2000)] with
minor
zs modifications. The RNA and DNA components of the scaffold (100 pmol of
each) were
mixed in 100 ~.1 of transcription buffer at 45 C and the mixture was allowed
to cool to
room temperature over 30 minutes. RNAP/scaffold complexes were formed by
incubation
of the annealed scaffold (10 pmol) with a molar equivalent of core RNAP
(either E.coli or
Taq) which was preincubated with rifampicin (100 p.M for E.coli, 200 p,M for
Taq) for 10
3 o minutes to form the RNAP/scaffold complex. Extension of the RNA
oligonucleotide was
assayed by the addition of a-[32P]CTP (0.3 wM) and a 5 minute incubation at
room
temperature. In Fig. 6b, lanes 1-5 and 16-20, RNAP was preincubated with
rifampicin (100
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CA 02440409 2003-09-09
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~M for E. coli RNAP, 200 ~.M for Taq) for 10 minutes. In lanes 6-10 and 21-25,
the
RNAP/scaffold complexes formed in the absence of rifampicin were incubated
with
rifampicin (concentrations as above) for 10 minutes. Finally, in lanes 11-15
and 26-30, the
RNAP or RNAP/scaffold complex was not exposed to rifampicin. Radioactive RNA
s products were analyzed on a 23% polyacrylamide sequencing gel.
Results
Rifanapicin Inhibition of Taq RNAP: From a biochemical perspective, the
interaction of rifampicin (Rif) with RNAP has been extensively characterized
using E. coli
RNAP, which served as a prototype for bacterial pathogens [Drancourt and
Raoult,
~.o Aratirnicn.AgentsChemotlzer. 43:2400-2403 (1999); Heep et al.,
Antimicf°.AgeratsClaemotlaer.
44:1075-1077 (1999); Honore et al., Molec.Microbiol. 7:207-214 (1993); Morse
et al.,
J.CIin.Mict-obiol, 37:2913-2929 (1999); Nolte, J.Atatinaicy~ob.Chemotlaer.
39:747-755(1997); Padayachee and Klugman, Antimicr.AgentsClzenaother. 43:2361-
2365
(1999); Ramaswamy and Musser, Tubercle anal Lung Disease 79:3-29 (1998);
Wichelhaus
i5 et al., AntinZicr.AgentsClZemoth.en. 43:2813-2816(1999)]. The inhibition of
Taq RNAP by
rifampicin was therefore investigated to assess this system as a structural
model for
Rif RNAP interactions. Sequence comparisons in the four distinct regions of
rpoB which
harbor RifR mutations indicate a very high level of conservation among
prokaryotes.
Between E. coli, Taq, and M. tubef~culosis, the sequences are 91 % identical
over 60 residues
a o (93% conserved), explaining the broad spectrum of rifampicin activity.
Nevertheless,
among the 23 positions with single amino-acid substitutions that give rise to
Rif in either
E. coli or M. tube~~culosis, 5 of these positions (Taq [3 387, 395, 398, 453,
and 566; the Taq
numbering is used throughout this application unless otherwise specified) are
substituted in
Taq (Fig. 1). In contrast, there is a relatively low level of conservation
between prokaryotes
a5 and eukaryotes within these regions (Fig. 1), explaining the lack of
rifampicin activity
against eukaryotic RNAPs and eukaryotic cells.
A plate assay (see Methods above) was used to show that Taq cells were unable
to
grow on media supplemented with 50 ~,g/ml rifampicin. For in vitro studies,
Taq RNAP
holoenzyme was reconstituted using Taq core RNAP purified from Taq cells
[Zhang et al.,
3 o Cell 98:811-824 (1999)] and recombinant Taq d4 (overexpressed and purified
from E. coli).
The enzyme initiated, elongated, and terminated transcripts efficiently from a
template
containing the T7A1 promoter and the tR2 intrinsic terminator (Fig. 2a)
[Nudler et al.,
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CA 02440409 2003-09-09
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J.Molec.Biol. 288:1-12(1994)] at 37 C using the dinucleotide CpA as the
initating primer.
The major RNA products, a trimeric abortive transcript (CpApLT), a 105
nucleotide
terminated transcript (Term), and a 127 nucleotide runoff transcript (Run
off), were the
same as those produced by E. coli RNAP (Fig. 2a, lanes 1 and 8). Since E. coli
6~° is totally
s inactive when combined with Taq core RNAP in this assay, the possibility of
trace
contamination with E. coli a~° does not affect the conclusions from
this assay for Taq
RNAP. Quantitatively, the two RNAPs responded very differently to rifampicin,
the Ki
(estimated from the rifampicin concentration where the production of long
transcripts was
inhibited by 50%) for E. coli RNAP was about 0.1 ~,M, while for Taq RNAP it
was about
20 10 ~,M, a 100-fold difference in sensitivity. Qualitatively, however, both
RNAPs responded
the same way, with an increase in the production of the trimeric product and a
concurrent
precipitous drop in the production of the long transcripts (Fig. 2a).
Mustaev et al., [Proc.Nat.Acad.Sci. USA 91:12036-12040 (1994)] used chimeric
Rif nucleotide compounds to measure the distance between the initiating
nucleotide binding
i5 site (the i-site) and the Rif binding site. By varying the linker between
the Rif and the
nucleotide and testing for maximal transcription initiation activity, the
optimal length was
found that allowed binding of each moiety in its respective site. This
experiment was used
to compare the disposition of the Rif and i-sites in E coli and Taq RNAP. In
both cases,
optimal initiation activity was observed when the linker comprised flue -(CH2)-
groups
z o (Figs. 2c-2d). Thus, in spite of the fact that Taq RNAP requires a 100-
fold higher
concentration of rifampicin fox inhibition, Taq RNAP binds rifampicin and is
inhibited
through the same biochemical mechanism as E. coli RNAP, and the disposition of
the
Rif site with respect to the universally conserved active site is identical.
Therefore, Taq
RNAP can serve as a model for rifampicin interactions with other RNAPs.
2s Rif-RNAP St~uctu~e Dete~sninatiora arad RefifZefraent: Tetragonal crystals
of Taq core
RNAP [Zhang et al., Cell 98:811-824 (1999)] were incubated overnight in
stabilization
buffer with 0.1 mM rifampicin, followed by a 30 minute soak in cryo-solution
(without
rifampicin) before flash freezing. During this procedure, the crystals took on
a deep orange
color, confirming the binding of rifampicin. The same results were obtained
with
3 o co-crystals grown in the presence of 0.1 mM rifampicin, suggesting that
rifampicin binding
causes few if any conformational changes in the RNAP.
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CA 02440409 2003-09-09
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The Taq core RNAP:Rif crystals were isomorphous with the native Taq core RNAP
crystals [Zhang et al., Cell 98:811-824 (1999)]. Strong electron density was
observed in
difference Fourier maps for the rifampicin (Fig. 3a), which occupies a shallow
pocket
between (3 structural domains 3 and 4 (Fig. 3b) that is surrounded by the
known Rif
mutations (Fig. 1) [Zhang et al., Cell 98:811-824 (1999)]. The electron
difference density
also indicated shifts and/or ordering of several [3 residues interacting
directly with
rifampicin, including Q390, L391, Q393, D396, H406, 8409, and L413 (Fig. 4).
Only very
small shifts in localized regions of the protein backbone were indicated.
The rifampicin X-ray crystal structure [Brufani et al., J. Molec.Biol. 87:409-
435
(1974)] was easily placed into the difference density. Subsequent refinements
resulted in
only small shifts of the ansa chain (Fig. 3c) to better fit the density.
Multiple rounds of
manual rebuilding against (2~F°I - ~F~~) maps and refinement resulted
in the current model
(see Methods above and Table 1).
Table 1
CRYSTALLOGRAPHIC DATA AND STRUCTURAL MODEL
DIFFRACTION DATA
a o Parameter Total Outer Shell
Resolution range (A) 30-3.3 3.42-3.3
Rmergel (%) 7.7 34.4
Completeness (%) 86.1 71.7
I/aI 10.7 1.7
2 s No. of reflections 75,420 6,173
No. of unique obs. 214,453 11,549
STRUCTURAL MODEL
3 o Number of Residues
ProteinMr (kDa) sequence model regions modeled
Subunit2
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CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
(3' 170.7 1,525 1,139 3-31, 69-155 (poly-Ala),
452-523,536-1241,
1250-1410, 1414-1497
/3 124.4 1,119 1,114 2-1115
aI 34.9 313 223 6-228
aII 34.9 313 229 3-231
11.6 99 98 1-98
1o Total 376.5 3,369 2,803
REFINEMENT
Rcryst (%) 28.1
Rfree (%) 35.9
lRmerge = E~Ij-<I>~/EIj
ZAlso included in the model was one Mg2~ and one Zn2+ ion [Zhang et al., Cell
98:811-824
(1999); U.S. Serial No. 09/396,651, Filed September 15, 1999] and one Rif
molecule
[Brufani et al., J. Molec.Biol. 87:409-435 (1974)].
z o Overall Structure: Consistent with the fact that all mapped Rife mutants
occur in
rpoB (Fig. 1), rifampicin makes contacts only with the RNAP [3 subunit in a
close
complementary fit to its binding pocket deep within the main DNA/RNA channel.
Clearly,
rifampicin does not bind directly at the RNAP active site (Fig. 3b). The
closest approach of
rifampicin to the active site, defined as the distance between the active site
Mg2+and C38 of
rifampicin (see Fig. 3c), is 12.1 ~.
Detailed Interactioyas: A large number of rifampicin derivatives have been
investigated for antimicrobial activity. In general, modification of the ansa
bridge, or
modifications that alter the conformation of the ansa bridge, reduce activity.
Other
structural features of the antibiotic that are particularly critical for
activity include the
3 o napthol ring with oxygen atoms (O1 and 02) at C 1 and C8, and
unsubstituted hydroxyls
(O10 and 09) at C21 and C23 (see Fig. 3c) [Arora, Acta Crystall. B37:152-157
(1981);
Arora, Molecular Plaarnaacology 23:133-140 (1983); Arora, J.Med. Claem.
28:1099-1102
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CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
(1985); Arora and Main, J. Ah.tibiot. 37:178-181 (1984); Brufani et al., J.
Molec.Biol.
87:409-435 (1974); Lancini and Zanichelli, In Structuf-e-activity
Relatiouslzip in
Sernisyrzthetic Azztibiotics, D. Perlaman, ed. (Academic Press), pp. 531-600
(1977); Sensi et
al., Rev.Infect.Dis., 5 Supp.3:402-406 (1983)]. Most rifampicin modifications
that retain
s activity involve substitutions at C3 of the napthol ring, which have only
modulatory effects
on izz vitro activity.
These results can be explained by the structural details of the Rif RNAP
complex
(Figs. 4a-4b and Sa-Sb). A cluster of hydrophobic residues (L391, L413, 6414,
I452) line
one wall of the Rif binding pocket and make van-der-Waals contact with the
napthol ring
1 o and the methyl group at C7. One end of the binding pocket (the bottom in
Figs. 4a-4b) is
formed by Q390. The alkyl chain of Q390 makes van-der-Waals contact with Rif
C28 and
C29, while the polar head group may interact with O5. Protein groups are
positioned to
make hydrogen bonds with each of the four critical hydroxyls of rifampicin:
8409 with O1,
Q393 and 5411 with 02, and D396 and H406 with 010. 09 and O10 are also in
position to
interact with the backbone amide and carboxyl of F394, respectively. 08 of
rifampicin is
also positioned to make a potential hydrogen bond with the backbone amide of
F394.
D396 contributes to the binding interface in several ways. In addition to
forming a
potential hydrogen bond with O10 of rifampicin, it forms the top end of the
binding pocket
(in Figs. 4a-4b) by making van-der-Waals contact with C18-C21, and C31.
Moreover, the
a o negative charge of D396 may be important for neutralizing the positive
charges of two
nearby side chains, 8405 and 8409 (Figs. 4a-4b), each about 6 ~ away. The
charge
neutralization might be important for the binding of the relatively apolar of
rifampicin.
Most Rif mutants at amino acid residue396 substitute a large, bulky group that
would
likely interfere with rifampicin binding and would not have the correct
geometry for
5 hydrogen bonding O10 (Y), or else substitute an apolar group (V, G, or A)
with no
hydrogen bonding ability. One of these mutants, D396V (amino acid position 516
in E.
coli), was among the original, strong Rif mutants mapped by Ovchinnikov et al.
[Molec. Gera. Gezzet.190:344-348 (1983)], pointing to the importance of this
residue in
forming the rifampicin binding interface. Another mutant identified in E.
coli, however
3 0 (D396N), is isosteric with aspartic acid and would likely maintain the
hydrogen bond with
O 10. Nevertheless, this substitution yields weak Rife [Lisitsyn et al., Bioof
g Klai»z
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CA 02440409 2003-09-09
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10:127-128 (1984)], which is likely caused by the loss of the negative charge
at this
position.
Rifampicin has a partial +-charge, localized at N4 (Fig. 3c). A negatively-
charged
residue, E445, is situated nearby and may contribute to the rifampicin binding
site by
s neutralizing this charge. This is not likely to be a strong effect, as many
rifampicin
derivatives with equal or stronger activity than rifampicin do not have this
partial charge.
E445 is the only residue close enough to rifampicin to be involved in
potentially direct
interactions (Figs. 4a-4b) for which a Rif mutant has not been reported.
However, this
residue is universally conserved as either glutamic acid or aspartic acid in a
segment of [3
i o region D that is invariantly present in prokaryotes, chloroplast,
archaebacteria, and
eukaryotes [Allison et al., Cell 42:599-610 (1985); Sweetser et al.,
P~oc.Natl.Acad.Sci. USA
84:1192-1196 (1987)], pointing to its importance for the basic function of
RNAP.
Thus, of the 12 residues that are close enough to rifampicin to make direct
interactions (including backbone interactions with F394; Figs. 4a-4b), 11
mutate to a Rii~
z5 phenotype. The twelfth position, E445, is highly conserved so that its
substitution would
likely be lethal and consequently not be detectable as a Rif mutation.
Twelve additional positions have been identified at which substitution gives
rise to
Rif (Fig. 1). These residues surround the Rif binding pocket but do not make
direct
interactions with the antibiotic (Figs. Sa-Sb). In every case, the Rif
mutations involve
z o replacement by a different sized amino acid side-chain (almost always
substituting a small
residue with a more bulky one), or else involve adding or removing a proline
residue.
These substitutions would likely affect the folding or packing of the protein
in the local
vicinity of the substituted residue, causing distortions of the Rif binding
pocket.
Mechanism of RNAP Inhibition. by Rif The effects of rifampicin on RNAP in each
a s stage of the transcription cycle have been probed using detailed kinetic
analyses.
Rifampicin has essentially no effect on specific promoter binding and open
complex
formation [Hinkle et al., J.Molec.Biol.,70, 209-220 (1972); McClure and Cech,
,LBiol.Chem. 253:8949-8956 (1978)]. A small increase (about 2-fold) in the
apparent Km
for initiating substrate binding in the enzyme's i-site (the 5'-nucleotide)
was observed, but
s o the binding of the incoming nucleotide substrate in the i+1 site (the 3'-
nucleotide), and the
formation of the first phosphodiester bond were largely unaffected [McClure
and Cech,
J.Biol.Chem. 253:8949-8956 (1978)]. The dominant effect of rifampicin binding
on RNAP
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CA 02440409 2003-09-09
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activity was a total blockage of synthesis of the second (when transcription
was initiated
with a nucleoside triphosphate) or third (when transcription was initiated
with a nucleoside
di- or monophosphate) phosphodiester bond [McClure and Cech, J.Biol.Claena.
253:8949-8956 (1978)]. Since s5mthesis of the first and second phosphodiester
bond can
s occur in the presence of rifampicin, the antibiotic does not interfere with
substrate binding,
catalytic activity, or the intrinsic translocation mechanism of the RNAP.
After RNAP has
synthesized a long transcript and entered the elongation phase, it becomes
totally resistant
to rifampicin. These properties led to the proposal that rifampicin inhibits
RNAP through a
simple steric block of the path of the elongating RNA at the 5'-end [McClure
and Cech,
io J.Biol.Chem. 253:8949-8956 (1978)]. Whether rifampicin directly blocked the
path of the
RNA, or if blockage was an indirect effect due to a conformational change in
the RNAP
induced by rifampicin binding, could not be distinguished. It has
alternatively been
proposed that rifampicin exerts its effect allosterically by decreasing the
affinity of the
RNAP for short RNA transcripts [Schulz and Zillig, Nucl.AcidsRes. 9:6889-6906
(1981)].
i 5 The Rif RNAP crystal structure explains the results described above and
strongly
supports the simple steric block mechanism, see, atomic coordinates included
in Table 2
[McClure and Cech, .LBiol.Chem. 253:8949-8956 (1978)]. Rifampicin directly
abuts the
base of a loop that comprises the C-terminal part of the /3 conserved region D
(amino acid
residues 443-451, shaded red in Figs. Sa-Sb), and a cluster of Rife mutants,
Rif cluster I
20 (Fig. 1), flanks this region. Modeling suggests that this loop, which
contains several nearly
universally conserved residues, participates in forming the binding site for
the base-pair at
+1 in the transcription complex [Korzheva et al., S'cieyace 289:619-625
(2000)], so effects of
rifampicin on the Km for the initiating substrate are not surprising. However,
rifampicin
does not directly contact the end of this loop. In addition, conformational
changes of the
a s protein in this region are not indicated from the structural data,
consistent with the
observation that the effect of rifampicin on this region is small.
The principal effect of rifampicin is seen in the context of a model of the
transcriptionally active ternary complex [Korzheva et al., Science 289:619-625
(2000)]
containing RNAP, DNA template, and RNA transcript (Fig. 6a). In Figure 6, only
the
3 o RNAP active site Mg2+ and the 9-basepair RNA/DNA hybrid (from +1 to -7)
from the
ternary complex model are shown. The rest of the RNAP and nucleic acids are
omitted for
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CA 02440409 2003-09-09
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clarity. Also shown is the atomic model of rifampicin as it would be
positioned in its
binding site on the (3 subunit.
It can be seen that the two substrate nucleotides, at +1 (green) and -1, are
not
directly affected by the presence of rifampicin so that RNAP can bind and
catalyze the
s formation of a phosphodiester bond between the two substrates in the
presence of the
antibiotic. With a transcript length of 3 nucleotides (nt), however, the 5'-
phosphates of the
5'-nucleotide (at -2) sterically clash with rifampicin, and the nucleotides
further upstream
(-3 to -5) severely clash with rifampicin. At the same time, rifampicin does
not interfere
with the DNA (grey). Thus, the structure, in combination with the ternary
complex model,
to explains the biochemical data on the mechanism of rifampicin inhibition,
provides strong
support for the proposal that rifampicin sterically blocks the path of the
elongating RNA
transcript at the 5'-end, and indicates that the blockage is a direct
consequence of rifampicin
binding in its site. The model further suggests why transcripts initiated with
nucleoside
triphosphates are blocked after the first phosphodiester bond, while
transcripts initiated with
s5 nucleoside di- or monophosphates are blocked after the second
phosphodiester bond. In the
model, the nucleoside monophosphate in the transcript at the -2 position
clashes only
slightly with rifampicin, while the presence of a 5'-triphosphate at the -2
position would
extend into rifampicin.
Core RNAP can bind a pre-formed 'minimal nucleic acid scaffold' of RNA/DNA
zo oligonucleotides (Fig. 6b, top) to yield functional ternary elongation
complexes [Korzheva
et al., Scieiace 289:619-625 (2000)]. Order of addition experiments were
performed using
this system in order to assess whether rifampicin and RNA binding were
competetive (Fig.
6b). The DNA component of the scaffold was annealed with varying lengths of
RNA
transcript, and the effect of rifampicin on the sequence-dependent extension
of RNA by one
z 5 nucleotide (radioactively-labeled CTP) added before or after the
oligonucleotides was
assayed at room temperature. In the case of E. coli core RNAP in the absence
of rifampicin
the RNA transcript was extended with nearly equal efficiency regardless of its
length within
a range of 3-7 nucleotides (Fig. 6b, lanes 11-1 S). When rifampicin was added
prior to the
nucleotide scaffold, the RNAP was unable to extend any of the RNA oligos,
regardless of
3 0 length (lanes 1-5), indicating that rifampicin occupied its site and
blocked the extension
and/or binding of all of the transcripts. When the scaffold was added prior to
rifampicin
addition, rifampicin was able to occupy its site and block the extension of
the 3-nucleotide
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CA 02440409 2003-09-09
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transcript (lane 6), but had no effect on the extension of the longer
transcripts (lanes 7-10),
presumably because rifampicin could not access its binding site due to the
presence of the
longer RNA transcripts (Fig. 6a). This result is consistent with the early
data that
rifampicin inhibits the RNA extension from 2 to 3 nucleotides if the 5'-
nucleoside is
tri-phosphorylated, but inhibits extension from 3 to 4 nucleotides if the 5'-
nucleoside is
mono- or di-phosphorylated [McClure and Cech, J.Biol.Claerra. 253:8949-8956
(1978)]
since the synthetic RNA oligos lack 5'-phosphates.
Similar experiments were performed with Taq core RNAP (Fig. 6b, lanes 16-30).
In
the absence of rifampicin, the efficiency of transcript extension was strongly
dependent on
i o the transcript length (lanes 26-30). Extension of the shortest transcripts
was barely
detectable, suggesting that, unlike E. coli RNAP, Taq, core RNAP does not bind
and
stabilize the short, intrinsically unstable RNA/DNA hybrids. In the presence
of rifampicin,
a generalized inhibition of transcript extension was observed regardless of
the order of
addition or of the transcript length (lanes 16-25). These results can be
explained by the low
i5 binding affinity of Taq core RNAP for both rifampicin and for short RNA
transcripts
compared with E. coli core RNAP. The low affinities imply fast off rates,
which would
allow equilibrium to be established between the rifampicin and scaffold
binding during the
time of the assay.
2 o Discussion
The 3.31 X-ray crystal structure of Taq core RNAP complexed with rifampicin is
disclosed herein. Though Taq RNAP is less sensitive to rifampicin than E coli
rifampicin,
at sufficiently high concentrations the antibiotic binds and inhibits the
enzyme.
Significantly, however, the inhibition of Taq RNAP by rifampicin occurs
through the same
2 s biochemical mechanism as E. coli RNAP, and the disposition of the Rif site
with respect to
the active site is identical to E. coli RNAP as well as with other prokaryotic
RNAPs (Figs.
2a-2d). Therefore, the structural information provided herein is relevant for
all bacteria
RNAPs.
The relative insensitivity of Taq RNAP to rifampicin is likely due to amino
acid
3 o substitutions in Taq RNAP compared with other, more Rif sensitive RNAPs.
The 12
residues close enough to interact directly with the rifampicin are identical
between E. coli,
Taq, and M. tuberculosis (marked yellow in Fig. 1 ). Among the 11 secondary
positions that
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CA 02440409 2003-09-09
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do not directly interact with rifampicin but likely affect rifampicin binding
indirectly, 5 are
substituted in Taq RNAP (amino acid residues 387, 395, 398, 453, and 566; Fig.
1). Three
of these positions, 387, 398, and 453, contain amino acids that are not
dramatically different
in overall size from their E. coli and M. tuberculosis counterparts and one
would predict
s that these residues are not the origin of the Taq RNAP insensitivity to
rifampicin. Position
566 is highly conserved among all RNAPs as either a lysine or an arginine (the
homologous
position is an arginine in both E. coli and M. tuberculosis) but is a
threonine in Taq RNAP.
This substitution is unlikely to be the main determinant of the Taq RNAP Rif
insensitivity,
however, since mutating Taq Thr566 to an arginine has little effect on the
RifR of the
z o enzyme when assayed at 45 C. This leaves position 395, which is highly
conserved as a
hydrophobic residue among all RNAPs. In E. coli and M. tuberculosis this
position is a
methionine, but in Taq it is a lysine. Taq Lys395 appears to participate in
buried
salt-bridges with Asp124 and Asp133 that may contribute to the thermostability
of the
protein. This non-conservative substitution (lysine for methionine) could
affect the local
15 path of the polypeptide backbone, and is immediately adjacent to Phe394,
the backbone
amide and carboxyl of which appear to be involved in important interactions
with the
rifampicin (Figs. 4a-4b).
All but one of the residues that are close enough to rifampicin to participate
in direct
interactions are known to mutate to strong Rif (Figs. 4a-4b). However,
additional residues
a o could be important for the formation of the Rif binding pocket but not
revealed as Rife
mutants if they are necessary for basic RNAP function. As mentioned above, the
four
regions of the (i subunit that harbor Rif mutants are highly conserved among
prokaryotes
(Fig. 1), but the much weaker homology with archaebacterial and eukaryotic
RNAPs,
combined with the fact that so many Rif mutations have been discovered,
indicate that
z s these regions are not critical to RNAP function in vivo. Nevertheless,
some Rife mutations
do have profound functional effects [Jin and Gross, J.BioI.C'hem. 266:14478-
14485 (1991);
Landick et al., Genes Develop. 4:1623-1636 (1990)], and E, coli strains with
RifR RNAP
have been shown to be at a competetive disadvantage to wild type E. coli in
the absence of
rifampicin [Jin and Gross, J.Bact.171:5229-5231 (1989)].
s o The clinical success of rifampicin proves that the bacterial RNAP is an
excellent
target for antimicrobials. The structure and available genetic and biochemical
data suggest
that the design of modified versions of rifampicin to overcome the effects of
Rife
-80-

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
mutations may lead to incremental improvements, though may not lead to a
Awonder= drug
because of the apparently small fiznctional penalties of mutating this region
of the RNAP,
and the variety of amino acid positions and mutations that result in Rif (Fig.
1). In
contrast, however, the findings from clinical isolates of RifR M. tuberculosis
are rather
encouraging. Thus, although the Rif mutations are spread over 15 positions of
rpoB, 77%
of all the mutations isolated involved substitutions at one of only two
positions,
corresponding to Taq amino acid residues 406 and 411. If a third amino acid
residue is
included, i.e., (Taq 396) a combined 86% of all the reported mutants are
accounted for.
One important conclusion from the present disclosure emerges regarding the
1 o inhibitory mechanism of rifampicin, i. e. , it is a simple steric block of
transcription
elongation. Thus, the powerful effects of rifampicin do not stem from the
details of its
chemical structure, and do not involve interference with the catalytic
activity of the RNAP,
e.g., by mimicking substrates or a transition state of the polymerization
reaction. Indeed,
such an inhibitor would likely act on features that are highly conserved
between prokaryotes
i5 and eukaryotes, rendering the inhibitor useless as an antimicrobial agent.
Rather, the effects
of rifampicin depend only on its ability to bind tightly to a relatively non-
conserved part of
the structure, disrupting a critical RNAP function by virtue of its presence.
Decades of
functional studies [Chamberlin, Harvey Lectures 88:1-21 (1993); Korzheva et
al.,Cold
Sp~irag Harbor Sy~raposia on. Quantitative Biology 63:337-345 (1998); Mustaev
et al.,
a o PYOC.Nat.Acad.Sci. USA 91:12036-12040 (1994); and Nudler, J.Molec.Biol.
288:1-I2
(1999)), and more recent structural evidence [framer et al., Science 288:640-
649 (2000);
Korzheva et al., Scie~zce 289:619-625 (2000); Mooney and Landick, Cell
98:687-690(1999); Zhang et al., Cell 98:811-824 (1999)) indicate that cellular
RNAPs
operate as complex molecular machines, with extensive interactions with the
template
2 s DNA, product RNA [Korzheva et al., Scieface 289:619-625 (2000)], and other
regulatory
molecules. Thus, many additional distinct sites exist where the tight binding
of a small
molecule (i.e., a novel antibiotic) would disrupt critical features of the
functional
mechanism of bacterial RNAPs. Such distinct sites can be readily identified
through the
structural information provided by the present invention.
3 o The present invention is not to be limited in scope by the specific
embodiments
describe herein. Indeed, various modifications of the invention in addition to
those
described herein will become apparent to those skilled in the art from the
foregoing
-81-

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
description and the accompanying figures. Such modifications are intended to
fall within
the scope of the appended claims.
It is further to be understood that all base sizes or amino acid sizes, and
all
molecular weight or molecular mass values, given for nucleic acids or
polypeptides are
approximate, and are provided for description.
Various publications are cited herein, the disclosures of which are
incorporated by
reference in their entireties.
WHAT IS CLAIMED IS:
-~2-

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
Table 2
REMARK Written by 0 version 6.2.1
REMARK Thu Oct 19 16:44:44 2000
CRYST1199.450 199.450 289.130 90.00 90.00
90.00
ORIGX1 1.000000 0. 0000000.000000 0.00000
ORIGX2 0.000000 1. 0000.000.000000 0.00000
ORIGX3 0.000000 0. 0000001.000000 0.00000
SCALE1 0.005014 0. 0000000.000000 0.00000
SCALE2 0.000000 0. 0050140.000000 0.00000
SCALES 0.000000 0. 0000000.003459 0.00000
ATOM 1 CB ALA A 6 -0.674 65.55973.225 1.00184.01
ATOM 2 C ALA A 6 -1.081 63.49271.882 1.00150.25
ATOM 3 0 ALA A 6 -1.961 62.63671.787 1.00150.75
ATOM 4 N ALA A 6 -2.906 65.12072.256 1.00150.94
ATOM 5 CA ALA A 6 -1.440 64.96072.050 1.00150.69
ATOM 6 N LYS A 7 0.220 63.20971.866 1.00121.92
ATOM 7 CA LYS A 7 0.729 61.85571.667 1.00120.02
ATOM 8 CB LYS A 7 -0.036 60.82172.496 1.00156.94
ATOM 9 CG LYS A 7 0.137 60.89073.994 1:00157.98
ATOM 10 CD LYS A 7 -0.665 59.76474.624 1.00159.08
ATOM 11 CE LYS A 7:~ -0.540 59.75076.131 1.00160.31
ATOM 12 NZ LYS A 7 -1.332 58.63876.731 1.00161.74
ATOM 13 C LYS A 7 0.489 61.55370.201 1.00118.48
ATOM 14 0 LYS A 7 1.116 60.66569.623 1.00118.87
ATOM 15 N ALA A 8 -0.435 62.30869.613 1.00 91.47
ATOM 16 CA ALA A 8 -0.801 62.14368.218 1.00 88.83
ATOM 17 CB ALA A 8 -2.077 61.30568.118 1.00 71.07
ATOM 18 C ALA A 8 -1.005 63.48767.524 1.00 86.40
ATOM 19 0 ALA A 8 -1.539 64.42868.112 1.00 86.27
A~'OM 20 N PRO A 9 -0.549 63.59766.267 1.00 53.91
' .
ATOM 21 CD PRO A 9 0.517 62.72265.741 1.00 69.31
ATOM 22 CA PRO A 9 -0.669 64.80965.449 1.00 52.02
ATOM 23 CB PRO A 9 0.674 64.86464.756 1.00 68.44
ATOM 24 CG PRO A 9 0.906 63.41264.453 1.00 69.17
ATOM 25 C PRO A 9 -1.824 64.69964.450 1.00 49.71
ATOM 26 0 PRO A 9 -2.494 63.67964.378 1.00 48.85
ATOM 27 N VAL A 10 -2.040 65.74663.670 1.00 56.71
ATOM 28 CA VAL A 10 -3.117 65.75362.692 1.00 55.46
ATOM 29 CB VAL A 10 -4.120 66.8.7263.012 1.00 55.12
ATOM 30 CG1 VAL A 10 -5.149 67.00561.904 1.00 55.17
ATOM 31 CG2 VAL A 10 -4.795 66.581.64.335 1.00 54.29
ATOM 32 C VAL A 10 -2.554 65.95861.296 1.00 55.42
ATOM 33 0 VAL A 10 -2.573 67.06460.756 1.00 55.98
.
ATOM 34 N PHE A 11 -2.069 64.87160.711 1.00 63.05
ATOM 35 CA PHE A 11 -1.454 64.90359.392 1.00 62.54
ATOM 36 CB PHE A 11 -0.919 63.50959.053 1.00119.96
ATOM 37 CG PHE A 11 -0.075 63.46057.809 1.00120.51
ATOM 38 CD1 PHE A 11 0.806 64.49457.500 1.00120.57
ATOM 39 CD2 PHE A 11 -0.135 62.35656.961 1.00121.37
ATOM 40 CE1 PHE A 11 1.616 64.42856.366 1.00119.73
ATOM 41 CE2 PHE A 11 0.669 62.28055.827 1.00120.93
ATOM 42 CZ PHE A 11 1.547 63.31855.527 1.00120.06
ATOM 43 C PHE A 11 -2.340 65.41958.266 1.00 62.10
ATOM 44 0 PHE A 11 -2.671 64.68557.338 1.00 61.58
ATOM 45 N THR A 12 -2.721 66.69058.355 1.00 47.43
ATOM 46 CA THR A 12 -3.533 67.33857.328 1.00 47.79
ATOM 47 CB THR A 12 -3.713 68.83457.647 1.00108.24

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 48 OG1 THR A 12 -4.382 68.979 58.909 1.00108.66
8
ATOM 49 CG2 THR A 12 -4.523 69.512 56.564 1.00109.46
6
ATOM 50 C THR A 12 -2.752 67.16 56.021 1.00 47.46
9 6
ATOM 51 0 THR A 12 -1.552 66.897 56.058 1.00 47.91
8
ATOM 52 N ALA A 13 -3.396 67.330 54.867 1.00 38.72
7
ATOM 53 CA .ALAA 13 -2.661 67.120 53.621 1.00 38.79
6
ATOM 54 CB ALA A 13 -2.509 65.590 53.374 1.00 19.61
6
ATOM 55 C ALA A 13 -3.163 67.803 52.350 1.00 38.10
6
ATOM 56 O ALA A 13 -3.149 67.194 51.295 1.00 37.13
8
ATOM 57 N THR A 14 -3.574 69.064 52.441 1.00 50.93
7
ATOM 58 CA THR A 14 -4.061 69.814 51.268 1.00 51.96
6
ATOM 59 CB THR A 14 -3.993 71.381 51.485 1.00 77.70
6
ATOM 60 OG1 THR A 14 -4.992 71.801 52.429 1.00 76.23
8
ATOM 61 CG2 THR A 14 -4.213 72.125 50.161 1.00 77.77
6
ATOM 62 C THR A 14 -3.253 69.477 50.007 1.00 52.16
6
ATOM 63 0 THR A 14 -2.238 70.113 49.713 1.00 51.78
8
ATOM 64 N THR A 15 -3.722 68.476 49.264 1.00 38.17
7
ATOM 65 CA THR A 15 -3.062 68.038 48.042 1.00 39.18
6
ATOM 66 CB THR A 15 -3.497 66.590 47.671 1.00 21.08
6
ATOM 67 OG1 THR A 15, -3.070 66.293 4'6.345 1.00 20.30
8
ATOM 68 CG2 THR A 15 -4.988 66.426 47.736 1.00 20.79
6
ATOM 69 C THR A 15 -3.301 68.987 46.863 1.00 39.71
6
ATOM 70 0 THR A 15 -3.672 70.150 47.049 1.00 38.75
8
ATOM 71 N GLN A 16 -3.060 68.477 45.659 1.00 87.11
7
ATOM 72 CA GLN A 16 -3.224 69.223 44.415 1.00 90.16
6
ATOM 73 CB GLN A 16 -2.064 70.199 44.226 1.00 98.47
6
ATOM 74 CG GLN A 16 -2.428 71.637 44.527 1.00 98.74
6
ATOM 75 CD GLN A 16 -3.310 72.240 43.459 1.00 98.04
6
ATOM 76 OE1 GLN A 16 -2.845 72.994 42.603 1.00 97.07
8
ATOM 77 NE2 GLN A 16 -4.591 71.900 43.494 1.00 98.68
7
ATOM 78 C GLN A 16 -3.274 68.263 43.229 1.00 91.21
6
ATOM 79 0 GLN A 16 -3.465 67.059 43.400 1.00 91.96
8
ATOM 80 N GLY A 17 -3.103 68.801 42.028 1.00109.33
7
ATOM 81 CA GLY A 17 -3.130 67.966 40.843 1.00110.69
6
ATOM 82 C GLY A 17 -1.741 67.744 40.276 1.00110.89
6
ATOM 83 0 GLY A 17 -1.408 68.262 39.208 1.00111.99
8
ATOM 84 N ASP A 18 -0.930 66.964 40.982 1.00 49.15
7
ATOM 85 CA ASP A 18 0.429 66.696 40.533 1.00 48.27
6
ATOM 86 CB ASP A 18 0.425 66.081 39.121 1.00 95.31
6
ATOM 87 CG ASP A 18 0.285 64.555 39.130 1.00 95.93
6
ATOM 88 OD1 ASP A 18 1.109 63.883 39.785 1.00 96.31
8
ATOM 89 OD2 ASP A 18 -0.639 64.026 38.474 1.00 95.54
' 8
ATOM 90 C ASP A 18 1.228 68.004 40.539 1.00 46.85
6
ATOM 91 O ASP A 18 1.470 68:615 39.496 1.00 46.35
8
ATOM 92 N HIS A 19 1.604 68.418 41.747 1.00 95.41
7
ATOM 93 CA HIS A 19 2.388 69.623 42.064 -1.0093.95
6
ATOM 94 CB HIS A 19 2.406 70.663 40.916 1.00119.86
6
ATOM 95 CG HIS A 19 1.071 71.252 40.570 1.00121.65
6
ATOM 96 CD2 HIS A 19 0.384 71.270 39.402 1.00121.92
6
ATOM 97 ND1 HIS A 19 0.319 71.982 41.465 1.00121.96
7
ATOM 98 CE1 HIS A 19 -0.772 72.424 40.864 1.00122.23
6
ATOM 99 NE2 HTS A 19 -0.756 72.005 39.611 1.00122.38
7
ATOM 100 C HIS A 19 1.835 70.218 43.363 1.00 91.34
6
ATOM 101 0 HIS A 19 0.882 69.678 43.940 1.00 90.88
8
ATOM 102 N TYR A 20 2.423 71.316 43.833 1.00 59.30
7
ATOM 103 CA TYR A 20 2.001 71.911 45.101 1.00 56.72
6
2

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 104 CB TYR A 20 0.675 72.641 44.992 1.00 84.02
6
ATOM 105 CG TYR A 20 0.845 74.124 44.855 1.00 86.19
6
ATOM 106 CD1 TYR A 20 0.937 74.719 43.593 1.00 86.75
6
ATOM 107 CE1 TYR A 20 1.114 76.095 43.451 1.00 87.24
6
ATOM 108 CD2 TYR A 20 0.936 74.940 45.982 1.00 87.02
6
ATOM 109 CE2 TYR A 20 1.115 76.321 45.852 1.00 87.64
6
ATOM 110 CZ TYR A 20 1.203 76.889 44.581 1.00 87.77
6
ATOM 111 OH TYR A 20 1.389 78.244 44.436 1.00 89.34
8
ATOM 112 C TYR A 20 1.847 70.778 46.072 1.00 53.80
6
ATOM 113 0 TYR A 20 2.391 69.707 45.867 1.00 54.73
8
ATOM 114 N GLY A 21 1.100 70.989 47.132 1.00 50.16
7
ATOM 115 CA GLY A 21 0.950 69.902 48.069 1.00 47.19
6
ATOM 116 C GLY A 21 1.471 70.248 49.442 1.00 44.51
6
ATOM 117 O GLY A 21 2.361 69.590 49.973 1.00 44.32
8
ATOM 118 N GLU A 22 0.924 71.314 50.005 1.00 39.88
7
ATOM 119 CA GLU A 22 1.293 71.724 51.335 1.00 37.92
6
ATOM 120 CB GLU A 22 0.488 72.965 51.716 1.00 62.99
6
ATOM 121 CG GLU A 22 0.217 73.904 50.537 1.00 64.94
6
ATOM 122 CD GLU A 22 -0.739 75.046 50.885 1.00 67.11
6
ATOM 123 OE1 GLU A 22 -1.688 74.806 51.671 1.00 67.85
8
ATOM 124 OE2 GLU A 22 -0.555 76.174 50.358 1.00 67.53
8
ATOM 125 C GLU A 22 0.843 70.511 52.145 1.00 35.14
6
ATOM 126 O GLU A 22 -0.221 69.953 51.886 1.00 35.06
8
ATOM 127 N PHE A 23 1.648 70.072 53.098 1.00 45.71
7
ATOM 128 CA PHE A 23 1.262 68.914 53.889 1.00 44.96
6
ATOM 129 CB PHE A 23 2.104 67.698 53.494 1.00 29.76
6
ATOM 130 CG PHE A 23 2.074 67.389 52.023 1.00 26.17
6
ATOM 131 CD1 PHE A 23 0.873 67.327 51.337 1.00 26.14
6
ATOM 132 CD2 PHE A 23 3.244 67.140 51.327 1.00 24.41
6
ATOM 133 CE1 PHE A 23 0.844 67.019 49.981 1.00 25.18
6
ATOM 134 CE2 PHE A 23 3.217 66.832 49.973 1.00 23.87
6
ATOM 135 CZ PHE A 23 2.020 66.773 49.304 1.00 24.25
6
ATOM 136 C PHE A 23 1.451 69.216 55.364 1.00 45.13
6
ATOM 137 O PHE A 23 2.564 69.127 55.880 1.00 46.47
8
ATOM 138 N VAL A 24 0.359 69.559 56.044 1.00 29.36
7
ATOM 139 CA VAL A 24 0.421 69.908 57.457 1.00 28.70
6
ATOM 140 CB VAL A 24 -0.710 70.840 57.891 1.00 26.37
6
ATOM 141 CG1 VAL A 24 -0.537 71.165 59.348 1.00 27.05
6
ATOM 142 CG2 VAL A 24 -0.716 72.115 57.074 1.00 26.62
6
ATOM 143 C VAL A 24 0.351 68.751 58.414 1.00 28.27
6
ATOM 144 O VAL A 24 -0.470 67.858 58.287 1.00 28.42
8
ATOM 145 N LEU A 25 1.236 68.776 59.390 1.00 24.98
7
ATOM 146 CA LEU A 25 1.223 67.767 60.413 1.00 25.10
6
ATOM 147 CB LEU A 25 2.600 67.635 61.051 1.00 20.57
6
ATOM 148 CG LEU A 25 2.998 66.180 61.263 1.00 20.16
6
ATOM 149 CD1 LEU A 25 3.158 65.901 62.743 1.00 18.63
6
ATOM 150 CD2 LEU A 25 1.941 65.264 60.635 1.00 19.90
6
ATOM 151 C LEU A 25 0.266 68.510 61.315 1.00 26.87
6
ATOM 152 O LEU A 25 -0.833 68.853 60.865 1.00 26.44
8
ATOM 153 N GLU A 26 0.669 68.787 62.555 1.00116.39
7
ATOM 154 CA GLU A 26 -0.183 69.547 63.467 1.00118.12
6
ATOM 155 CB GLU A 26 -1.494 68.801 63.727 1.00 55.30
6
ATOM 156 CG GLU A 26 -2.743 69.596 63.350 1.00 53.07
6
ATOM 157 CD GLU A 26 -2.547 71.087 63.477 1.00 51.49
6
ATOM 158 OE1 GLU A 26 -2.151 71.538 64.566 1.00 49.62
8
ATOM 159 OE2 GLU A 26 -2.795 71.803 62.486 1.00 52.84
8
3

CA 02440409 2003-09-09
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ATOM 160 C GLU A 26 0.466 69.999 64.786 1.00120.58
6
ATOM 161 O GLU A 26 1.435 70.758 64.754 1.00121.90
8
ATOM 162 N PRO A 27 -0.027 69.541 65.959 1.00 79.42
7
ATOM 163 CD PRO A :27 -0.943 68.451 66.354 1.00 84.24
6
ATOM 164 CA PRO A 27 0.660 70.056 67.143 1.00 79.50
6
ATOM 165 CB PRO A 27 -0.342 69.790 68.241 1.00 83.86
6
ATOM 166 CG PRO A 27 -0.787 68.400 67.874 1.00 84.43
6
ATOM 167 C PRO A 27 1.957 69.323 67.388 1.00 81.30
6
ATOM 168 O PRO A 27 1.970 68.305 68.076 1.00 82.18
8
ATOM 169 N LEU A 28 3.045 69.827 66.819 1.00 52.55
7
ATOM 170 CA LEU A 28 4.337 69.190 67.014 1.00 53.38
6
ATOM 171 CB LEU A 28 5.204 69.324 65.756 1.00 30.51
6
ATOM 172 CG LEU A 28 5.135 68.133 64.788 1.00 29.10
6
ATOM 173 CD1 LEU A 28 5.692 68.542 63.433 1.00 27.98
6
ATOM 174 CD2 LEU A 28 5.892 66.924 65.380 1.00 28.36
6
ATOM 175 C LEU A 28 5.014 69.835 68.203 1.00 55.09
6
ATOM 176 O LEU A 28 4.938 71.047 68.383 1.00 54.89
8
ATOM 177 N GLU A 29 5.652 69.011 69.028 1.00 95.04
7
ATOM 178 CA GLU A 29 6.341 69.510 70.205 1.00 97.70
6
ATOM 179 CB GLU A 29 7.201 68.411 70.835 1.00135.72
6
ATOM 180 CG GLU A 29 6.414 67.210 71.331 1.00139.75
6
ATOM 181 CD GLU A 29 7.248 66.273 72.187 1.00142.12
6
ATOM 182 OE1 GLU A 29 7.820 66.742 73.197 1.00144.19
8
ATOM 183 OE2 GLU A 29 7.324 65.067 71.860 1.00142.16
8
ATOM 184 C GLU A 29 7.221 70.683 69.806 1.00 98.60
6
ATOM 185 O GLU A 29 7.888 70.652 68.770 1.00 98.86
8
ATOM 186 N ARG A 30 7.206 71.729 70.620 1.00 49.18
7
ATOM 187 CA ARG A 30 8.021 72.900 70.349 1.00 48.17
6
ATOM 188 CB ARG A 30 8.029 73.811 71.573 1.00135.75
6
ATOM 189 CG ARG A 30 7.710 73.099 72.883 1.00138.35
6
ATOM 190 CD ARG A 30 6.214 72.839 73.015 1.00140.35
6
ATOM 191 NE ARG A 30 5.858 72.349 74.342 1.00141.60
7
ATOM 192 CZ ARG A 30 4.676 72.544 74.917 1.00142.62
6
ATOM 193 NH1 ARG A 30 3.733 73.224 74.282 1.00143.40
7
ATOM 194 NH2 ARG A 30 4.434 72.060 76.128 1.00142.68
7
ATOM 195 C ARG A 30 9.449 72.499 69.996 1.00 47.03
6
ATOM 196 O ARG A 30 10.265 72.211 70.879 1.00 45.83
8
ATOM 197 N GLY A 31 9.746 72.474 68.704 1.00 86.21
7
ATOM 198 CA GLY A 31 11.082 72.113 68.289 1.00 85.50
6
ATOM 199 C GLY A 31 11.183 71.239 67.060 1.00 84.90
6
ATOM 200 0 GLY A 31 11.913 71.569 66.127 1.00 85.07
8
ATOM 201 N PHE A 32 10.459 70.123 67.051 1.00 67.60
7
ATOM 202 CA PHE A 32 10.508 69.204 65.918 1.00 66.18
6
ATOM 203 CB PHE A 32 9.686 67.948 66.221 1.00 37.90
6
ATOM 204 CG PHE A 32 10.366 66.995 67.163 1.00 36.46
6
ATOM 205 CD1 PHE A 32 11.292 67.448 68.090 1.00 36.18
6
ATOM 206 CD2 PHE A 32 10.057 65.645 67.154 1.00 35.75
6
ATOM 207 CE1 PHE A 32 11.897 66.557 68.998 1.00 36.60
6
ATOM 208 CE2 PHE A 32 10.662 64.746 68.066 1.00 35.33
6
ATOM 209 CZ PHE A 32 11.573 65.200 68.979 1.00 35.10
6
ATOM 210 C PHE A 32 10.056 69.845 64.615 1.00 65.97
6
ATOM 211 O PHE A 32 10.196 69.254 63.546 1.00 65.84
8
ATOM 212 N GLY A 33 9.534 71.063 64.704 1.00 77.10
7
ATOM 213 CA GLY A 33 9.097 71.751 63.507 1.00 75.91
6
ATOM 214 C GLY A 33 10.094 71.568 62.376 1.00 75.60
6
ATOM 215 O GLY A 33 9.720 71.510 61.206 1.00 76.44
8
4

CA 02440409 2003-09-09
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ATOM 216 N VAL A 34 11.372 71.467 62.725 1.00 52.80
7
ATOM 217 CA VAL A 34 12.410 71.289 61.724 1.00 51.83
6
ATOM 218 CB VAL A 34 13.501 72.380 61.800 1.00 59.73
6
ATOM 219 CG1 VAL A 34 12.909 73.749 61.479 1.00 60.67
6
ATOM 220 CG2 VAL A 34 14.141 72.363 63.168 1.00 61.37
6
ATOM 221 C VAL A 34 13.097 69.951 61.856 1.00 50.63
,6
ATOM 222 O VAL A 34 13.579 69.422 60.863 1.00 51.62
8
ATOM 223 N THR A 35 13.154 69.392 63.063 1.00 36.90
7
ATOM 224 CA THR A 35 13.819 68.095 63.226 1.00 35.09
6
ATOM 225 CB THR A 35 13.474 67.373 64.559 1.00 52.73
6
ATOM 226 OG1 THR A 35 13.693 68.247 65.671 1.00 53.28
8
ATOM 227 CG2 THR A 35 14.371 ' 66.16864.733 1.00 52.98
6
ATOM 228 C THR A 35 13.309 67.240 62.088 1.00 32.97
6
ATOM 229 O THR A 35 14.026 66.415 61.536 1.00 32.07
8
ATOM 230 N LEU A 36 12.056 67.471 61.728 1.00 20.88
7
ATOM 231 CA LEU A 36 11.432 66.739 60.649 1.00 21.18
6
ATOM 232 CB LEU A 36 10.009 66,343 61.051 1.00 39.45
6
ATOM 233 CG LEU A 36 9.755 65.996 62.526 1.00 39.89
6
ATOM 234 CD1 LEU A 36 8.258 65.818 62.752 1.00 41.19
6
ATOM 235 CD2 LEU A 36 10.515 64.740 62.926 1.00 39.22
6
ATOM 236 C LEU A 36 11.397 67.668 59.442 1.00 21.63
6
ATOM 237 O LEU A 36 11.850 67.319 58.357 1.00 20.73
8
ATOM 238 N GLY A 37 10.875 68.868 59.666 1.00 54.11
7
ATOM 239 CA GLY A 37 10.742 69.862 58.616 1.00 56.99
6
ATOM 240 C GLY A 37 11.866 70.059 57.615 1.00 58.77
6
ATOM 241 O GLY A 37 11.597 70.381 56.463 1.00 60.64
8
ATOM 242 N ASN A 38 13.118 69.880 58.019 1.00 43.74
7
ATOM 243 CA ASN A 38 14.211 70.089 57.080 1.00 42.89
6
ATOM 244 CB ASN A 38 15:385 70.783 57.777 1.00 40.56
6
ATOM 245 CG ASN A 38 16.115 71.759 56.864 1.00 39.64
6
ATOM 246 OD1 ASN A 38 16.918 71.376 56.016 1.00 38.98
8
ATOM 247 ND2 ASN A 38 15.820 73.033 57.032 1.00 40.13
7
ATOM 248 C ASN A 38 14.693 68.826 56.362 1.00 42.40
6
ATOM 249 O ASN A 38 14.521 68.720 55.145 1.00 41.83
8
ATOM 250 N PRO A 39 15.282 67.851 57.094 1.00 40.48
7
ATOM 251 CD PRO A 39 15.267 67.679 58.555 1.00 16.97
6
ATOM 252 CA PRO A 39 15.770 66.620 56.463 1.00 41.24
6
ATOM 253 CB PRO A 39 15.764 65.618 57.618 1.00 16.06
6
ATOM 254 CG PRO A 39 16.131 66.439 58.747 1.00 15.86
6
ATOM 255 C PRO A 39 14.901 66.170 55.286 1.00 42.78
6
ATOM 256 0 PRO A 39 15.411 65.649 54.290 1.00 42.03
8
ATOM 257 N LEU A 40 13.589 66.375 55.408 1.00 77.92
7
ATOM 258 CA LEU A 40 12.657 66.004 54.351 1.00 79.48
6
ATOM 259 CB LEU A 40 11.207 66.157 54.825 1.00 44.82
6
ATOM 260 CG LEU A 40 10.797 65.405 56.093 1.00 45.44
6
ATOM 261 CD1 LEU A 40 9.303 65.573 56.318 1.00 45.40
6
ATOM 262 CD2 LEU A 40 11.155 63.930 55.977 1.00 45.49
6
ATOM 263 C LEU A 40 12.906 66.921 53.165 1.00 80.46
6
ATOM 264 0 LEU A 40 13.203 66.466 52.057 1.00 80.99
8
ATOM 265 N ARG A 41 12.793 68.221 53.408 1.00 78.27
7
ATOM 266 CA ARG A 41 13.010 69.201 52.359 1.00 77.86
6
ATOM 267 CB ARG A 41 12.950 70.615-52.948 1.00 71.76
6
ATOM 268 CG ARG A 41 12.558 71.705 51.953 1.00 73.75
6
ATOM 269 CD ARG A 41 13.741 72.275 51.194 1.00 74.31
6
ATOM 270 NE ARG A 41 14.589 73.075 52.066 1.00 76.34
7
ATOM 271 CZ ARG A 41 15.454 72.579 52.945 1.00 79.31
6
S

CA 02440409 2003-09-09
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ATOM 272 NH1 ARG A 41 15.606 71.270 53.079 1.00 80.51 7
ATOM 273 NH2 ARG A 41 16.164 73.396 53.708 1.00 79.89 7
ATOM 274 C ARG A 41 14.376 68.910 51.748 1.00 76.52 6
ATOM 275 0 ARG A 41 14.559 68.987 50.534 1.00 76.69 8
ATOM 276 N ARG A 42 15.333 68.559 52.598 1.00 55.69 7
ATOM 277 CA ARG A 42 16.665 68.228 52.117 1.00 54.23 6
ATOM 278 CB ARG A 42 17.559 67.759 53.266 1.00 43.27 6
ATOM 279 CG ARG A 42 17.965 68.846 54.226 1.00 43.46 6
ATOM 280 CD ARG A 42 19.175 69.576 53.713 1.00 42.80 6
ATOM 281 NE ARG A 42 20.400 68.918 54.144 1.00 41.66 7
ATOM 282 CZ ~ARGA 42 21.593 69.162 53.617 1.00 41.52 6
ATOM 283 NH1 ARG A 42 21.714 70.050 52.635 1.00 40.62 7
ATOM 284 NH2 ARG A 42 22.663 68.522 54.074 1.00 40.40 7
ATOM 285 C ARG A 42 16.490 67.094 51.127 1.00 52.39 6
ATOM 286 0 ARG A 42 16.635 67.289 49.921 1.00 52.13 8
ATOM 287 N II,EA 43 16.150 65.914 51.650 1.00 28.41 7
ATOM 288 CA ILE A 43 15.961 64.731 50.827 1.00 25.56 6
ATOM 289 CB ILE A 43 ' 15.265 63.585 51.616 1.00 20.13 6
ATOM 290 CG2 ILE A 43 15.289 62.291 50.806 1.00 18.45 6
ATOM 291 CG1 ILE A 43 16.002 63.313 52.926 1.00 19.25 6
ATOM 292 CD1 ILE A 43 17.358 62.780 52.730 1.00 20.64 6
ATOM 293 C ILE A 43 15.122 65.096 49.611 1.00 24.61 6
ATOM 294 O ILE A 43 15.596 64.997 48.483 1.00 23.15 8
ATOM 295 N LEU A 44 13.891 65.538 49.834 1.00 60.43 7
ATOM 296 CA LEU A 44 13.022 65.900 48.721 1.00 62.04 6
ATOM 297 CB LEU A 44 11.928 66.843 49.205 1.00 32.70 6
ATOM 298 CG LEU A 44 10.914 66.119 50.078 1.00 31.74 6
ATOM 299 CD1 LEU A 44 10.156 67.122 50.898 1.00 32.66 6
ATOM 300 CD2 LEU A 44 9.969 65.310 49.219 1.00 31.99 6
ATOM 301 C LEU A 44 13.770 66.526 47.539 1.00 62.79 6
ATOM 302 O LEU A 44 13.569 66.130 46.387 1.00 63.37 8
ATOM 303 N LEU A 45 14.645 67.487 47.828 1.00 54.74 7
ATOM 304 CA LEU A 45 15.411 68.169 46.789 1.00 52.16 6
ATOM 305 CB LEU A 45 16.062 69.423 47.363 1.00 13.87 6
ATOM 306 CG LEU A 45 15.152 70.604 47.623 1.00 13.87 6
ATOM 307 CD1 LEU A 45 15.843 71.586 48.516 1.00 13.87 6
ATOM 308 CD2 LEU A 45 14.792 71.238 46.320 1.00 13.87 6
ATOM 309 C LEU A 45 16.494 67.306 46.167 1.00 52.20 6
ATOM 310 O LEU A 45 16.788 67.441 44.980 1.00 51.60 8
ATOM 311 N SER A 46 17.074 66.423 46.977 1.00 47.04 7
ATOM 312 CA SER A 46 18.169 65.550 46.556 1.00 47.85 6
ATOM 313 CB SER A 46 19.098 65.282 47.734 1.00 62.29 6
ATOM 314 OG SER A 46 19.440 66.478 48.399 1.00 64.36 8
ATOM 315 C SER A 46 17.793 64.207 45.965 1.00 47.22 6
ATOM 316 O SER A 46 17.335 64.098 44.833 1.00 47.52 8
ATOM 317 N SER A 47 18.015 63.171 46.754 1.00 19.54 7
ATOM 318 CA SER A 47 17.740 61.819 46.314 1.00 20.83 6
ATOM 319 CB SER A 47 18.339 60.840 47.330 1.00 47.90 6
ATOM 320 OG SER A 47 19.636 61.245 47.740 1.00 48.74 8
ATOM 321 C SER A 47 16.240 61.523 46.111 1.00 20.87 6
ATOM 322 O SER A 47 15.415 61.785 46.986 1.00 21.06 8
ATOM 323 N ILE A 48 15.900 60.977 44.953 1.00 46.59 7
ATOM 324 CA ILE A 48 14.528 60.621 44.624 1.00 48.06 6
ATOM 325 CB ILE A 48 13.512 61.749 44.878 1.00 13.87 6
ATOM 326 CG2 ILE A 48 12.415 61.715 43.809 1.00 13.87 6
ATOM 327 CG1 ILE A 48 12.859 61.582 46.244 1.00 13.87 '6
6

CA 02440409 2003-09-09
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ATOM 328 CD1 ILEA 48 11.777 62.610 46.523 1.00 13.87 6
ATOM 329 C ILEA 48 14.472 60.333 43.151 1.00 50.88 6
ATOM 330 0 ILEA 48 14.926 61.135 42.337 1.00 52.63 8
ATOM 331 N PROA 49 13.902 59.185 42.783 1.00 39.58 7
ATOM 332 CD PROA 49 13.394 58.110 43.655 1.00145.29 6
ATOM 333 CA PROA 49 13.797 58.814 41.377 1.00 39.89 6
ATOM 334 CB PROA 49 12.979 57.525 41.428 1.00145.15 6
ATOM 335 CG PROA 49 13.408 56.916 42.729 1.00146.08 6
ATOM 336 C PROA 49 13.122 59.903 40.544 1.00 40.28 6
ATOM 337 0 PROA 49 12.250 60.639 41.028 1.00 39.98 8
ATOM 338 N GLYA 50 13.547 59.985 39.286 1.00 41.27 7
ATOM 339 CA GLYA 50 13.005 60.947 38.349 1.00 40.92 6
ATOM 340 C GLYA 50 13.190 60.493 36.911 1.00 39.90 6
ATOM 341 0 GLYA 50 13.762 59.439 36.615 1.00 38.85 8
ATOM 342 N THRA 51 12.704 61.315 36.001 1.00 41.55 7
ATOM 343 CA THRA 51 12.794 61.010 34.593 1.00 42.82 6
ATOM 344 CB THRA 51 11.383 60.828 33.998 1.00 61.58 6
ATOM 345 OG1 THRA 51 10.709 62.095 33.965 1.00 63.80 8
ATOM 346 CG2 THRA 51 10.569 59.864 34.849 1.00 62.73 6
ATOM 347 C THRA 51 13.482 62.176 33.890 1.00 41.48 6
ATOM 348 0 THRA 51 13.023 63.323 33.985 1.00 40.65 8
ATOM 349 N ALAA 52 14.584 61.893 33.194 1.00 29.66 7
ATOM 350 CA ALAA 52 15.298 62.94 32.469 1.00 30.42 6
1
ATOM 351 CB ALAA 52 16.441 63.462 33.312 1.00 77.76 6
ATOM 352 C ALAA 52 15.823 62.519 31.106 1.00 29.19 6
ATOM 353 0 ALAA 52 16.153 61.364 30.883 1.00 27.97 8
ATOM 354 N VALA 53 15.887 63.478 30.195 1.00 33.16 7
ATOM 355 CA VALA 53 16.400 63.247 28.849 1.00 33.88 6
ATOM 356 CB VALA 53 16.381 64.569 28.066 1.00 31.89 6
ATOM 357 CG1 VALA 53 16.599 64.340 26.574 1.00 31.71 6
ATOM 358 CG2 VALA 53 15.084 65.270 28.335 1.00 31.88 6
ATOM 359, C VALA 53 ~ 17.856 62.776 28.970 1.00 34.18 6
ATOM 360 0 VALA 53 18.671 63.447 29.595 1.00 34.70 8
ATOM 361 N THRA 54 18.212 61.645 28.382 1.00 53.17 7
ATOM 362 CA THRA 54 19.596 61.222 28.518 1.00 55.64 6
ATOM 363 CB THRA 54 19.689 59.742 28.906 1.00 54.68 6
ATOM 364 OG1 THRA 54 19.168 59.570 30.227 1.00 55.11 8
ATOM 365 CG2 ,THRA 54 21.141 59.269 28.885 1.00 55.71 6
ATOM 366 C THRA 54 20.464 61.470 27.294 1.00 56.10 6
ATOM 367 0 THRA 54 21.642 61.817 27.437 1.00 56.71 8
ATOM 368 N SERA 55 19.881 61.302 26.102 1.00 42.59 7
ATOM 369 CA SERA 55 20.595 61.492 24.830 1.00 43.27 6
ATOM 370 CB SERA 55 21.444 60.253 24.536 1.00 30.83 6
ATOM 371 OG SERA 55 22.262 60.453 23.409 1.00 31.95 8
ATOM 372 C SERA 55 19.624 61.754 23.669 1.00 42.60 6
ATOM 373 0 SERA 55 18.516 61.222 23.641 1.00 41.99 8
ATOM 374 N VALA 56 20.042 62.567 22.707 1.00 49.56 7
ATOM 375 CA VALA 56 19.167 62.893 21.585 1.00 51.39 6
ATOM 376 CB VALA 56 18.463 64.291 21.818 1.00 18.02 6
ATOM 377 CG1 VALA 56 18.759 64.775 23.223 1.00 17.77 6
ATOM 378 CG2 VALA 56 18.899 65.343 20.784 1.00 15.68 6
ATOM 379 C VALA 56 19.843 62.892 20.219 1.00 52.77 6
ATOM 380 0 VALA 56 20.836 63.586 20.009 1.00 53.75 8
ATOM 381 N TYRA 57 19.306 62.103 19.292 1.00 49.16 7
ATOM 382 CA TYRA 57 19.842 62.065 17.935 1.00 50.62 6
ATOM 383 CB TYRA 57 19.973 60.627 17.431 1.00 56.89 6
7

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
384 CG TYR 57 20.071 60.507 15.908 1.00 55.88 6
A
ATOM CD1TYR 57 21.230 60.024 15.295 1.00 55.45 6
A
ATOM 385 5~7 305 879 13.911 1.00 54.69 6
21 59
ATOM 386 CE1TYR 57 . . 15.084 1.00 55.26 6
A 18 848
991 60
ATOM 387 CD2TYR 57 . . 13.710 1.00 54.91 6
A 061 710
19 60
ATOM 388 CE2TYR 57 . . 13.130 1.00 54.94 6
A 216 222
20 60
ATOM 389 CZ TYR 57 . . 11.770 1.00 55.85 8
A 270 047
20 60
ATOM 390 OH TYR 57 . . 16.977 1.00 51.34 6
A 933 836
18 62
ATOM 391 C TYR 57 . . 16.997 1.00 51.30 8
A 713 680
17 62
ATOM 392 O TYR 58 . . 16.120 1.00 48.33 7
A 529 651
19 63
ATOM 393 N ILE 58 . . 15.160 1.00 50.45 6
A 738 390
18 64
ATOM 394 CA ILE . . 15.341 1.00 43.10 6
A 904 906
18 65
ATOM 395 CB ILE 58 . . 14.254 1.00 42.04 6
2 A 58 130 66.651
A 18
ATOM 396 CG ILE 58 . 317 16.712 1.00 42.84 6
A 379 66
18
ATOM 397 CG1ILE 58 . . 16.816 1.00 44.49 6
051 801
18 67
ATOM 398 CD1ILE . . 733 1.00 51.73 6
A 107 991 13
19 63
ATOM 399 C ILE 58 . . . 1.00 50.59 8
A 58 239 590 13.457
20 63
ATOM 400 O ILE 59 . . 12.841 1.00 92.68 7
A 127 110
A 18 64
ATOM 401 N GLU 59 . . 11.430 1.00 95.41 6
267 761
18 63
ATOM 402 CA GLU 59 . . 10.598 1.00103.73 6
A 209 64.501
LU A 17
ATOM 403 CB G 59 . 033 9.140 1.00105.12 6
103 64
17
ATOM 404 CG GLU 59 . . 8.974 1.00106.04 6
A 435 663
16 62
ATOM 405 CD GLU 59 . . 9.571 1.00106.23 8
A 917 675
A 16 61
ATOM 406 OE1GLU 59 . . 8.234 1.00106.36 8
429 569
15 62
ATOM 407 OE2GLU 59 . . 10.836 1.00 95.22 6
A 646 019
19 64
ATOM 408 C GLU 59 . . 11.038 1.00 95.27 8
A 574 238
20 63
ATOM 409 O GLU 60 . . 10.104 1.00 54.07 7
A 776 119
19 65
ATOM 410 N ASP 60 . . 9.451 1.00 54.95 6
A 039 455
21 65
ATOM 411 CA ASP 60 . . 8.148 1.00121.45 6
A 755 216
20 66
ATOM 412 CB ASP 60 . . 7.130 1.00123.20 6
A 988 380
19 65
ATOM 413 CG ASP 60 . . 6.672 1.00124.19 8
A 519 343
20 64
ATOM 414 OD1ASP . . 778 1.00123.59 8
A 851 760 6
18 65
ATOM 415 OD2ASP 60 . . . 1.00 54.33 6
A 016 261 311
22 66 10
ATOM 416 C ASP 60 . . . 1.00 54.17 8
A 60 484 321 9.896
22 67
ATOM 417 O ASP 61 . . 11.496 1.00 54.31 7
A 351 757
22 65
ATOM 418 N VAL 61 . . 12.363 1.00 55.07 6
A 268 491
23 66
ATOM 419 CA VAL 61 . . 13.511 1.00 62.32 6
A 507 192
22 67
ATOM 420 CB VAL 61 . . 14.504 1.00 62.29 6
A 498 801
23 67
ATOM 421 CG1VAL 61 . . 12.939 1.00 62.30 6
A 600 275
21 68
ATOM 422 CG2VAL 61 . . 12.970 1.00 54.97 6
A 389 663
24 65
ATOM 423 C VAL 61 . . 13.808 1.00 54.94 8
A 152 798
24 64
ATOM 424 0 VAL 62 . . 12.552 1.00 73.57 7
A 614 965
A 25 65
ATOM 425 N LEU 62 . . 13.040 1.00 74.33 6
790 274
26 65
ATOM 426 CA LEU 62 . . 12.438 1.00 68.22 6
A 035 900
28 65
ATOM 427 CB LEU 62 . . 11.138 1.00 68.33 6
A 478 236
28 65
ATOM 428 CG LEU 62 . . 10.432 1.00 68.18 6
A 514 087
29 66
ATOM 429 CD1 62 . . 11.463 1.00 68.59 6
LEU 033 854
A 29 63
A
ATOM 430 CD2LEU 62 . . 14.554 1.00 74.75 6
891 266
26 65
ATOM 431 C LEU 62 . . 15.162 1.00 75.89 8
A 801 211
26 64
ATOM 432 O LEU 63 . . 15.162 1.00 68.14 7
A 095 430
27 66
ATOM 433 N HIS 63 . . 16.622 1.00 68.73 6
A 188 533
' 27 66
ATOM 434 CA HIS 63' . . 17.112 1.00109.32 6
A 612 247
28 66
ATOM 435 CB HIS 63 . . 17.598 1.00111.08 6
A 817 842
28 64
ATOM 436 CG 63 . . 17.545 1.00111.66 6
HIS 015 753
A 28 63
ATOM 437 CD2 63 . . 18:234 1.00112.16 7
HIS 970 436
A 29 64
ATOM 438 ND1 . . 552 1.00112.77 6
HIS 868 157 18
A 29 63
ATOM 439 CE 1 HIS 63 . . .
A
8

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 440 NE2 HIS A 63 28.692 62.719 18.144 1.00112.76
ATOM 441 C HIS A 63 26.760 67.921 17.067 1.00 67.23
ATOM 442 0 HIS A 63 26.497 68.774 16.225 1.00 67.52
ATOM 443 N GLU A 64 26.687 68..14518.380 1.00 95.10
ATOM 444 CA GLU A 64 26.269 69.438 18.922 1.00 93.64
ATOM 445 CB GLU A 64 26.480 69.480 20.439 1.00 79.44
ATOM 446 CG GLU A 64 25.731 70.615 21.143 1.00 80.88
ATOM 447 CD GLU A 64 25.248 70.242 22.554 1.00 81.25
ATOM 448 OE1 GLU A 64 25.604 69.147 23.047 1.00 81.28
ATOM 449 OE2 GLU A 64 24.508 71.045 '23.174 1.00 81.64
ATOM 450 C GLU A 64 27.054 70.548 18.240 1.00 91.41
ATOM 451 O GLU A 64 28.046 70.278 17.562 1.00 92.49
ATOM 452 N PHE A 65 26.617 71.791 18.420 1.00 22.51
ATOM 453 CA PHE A 65 27.266 72.948 17.782 1.00 20.38
ATOM 454 CB PHE A 65 28.801 72.924 17.954 1.00 28.12
ATOM 455 CG PHE A 65 29.236 72.710 19.363 1.00 24.54
ATOM 456 CD1 PHE A 65 29.535 71.434 19.827 1.00 22.93
ATOM 457 CD2 PHE A 65 29.224 73.757 20.267 1.00 23.17
ATOM 458 CE1 PHE A 65 29.804 71.197 21.170 1.00 21.54
ATOM 459 CE2 PHE A 65 29.489 73.530 21.616 1.00 21.40
ATOM 460 CZ PHE A 65 29.777 72.247 22.062 1.00 20.54
ATOM 461 C PHE A 65 26.924 72.930 16.294 1.00 19.54
ATOM 462 0 PHE A 65 27.787 73.189 15.447 1.00 17.93
ATOM 463 N SER A 66 25.660 72.621 15.990 1.00107.08
ATOM 464 CA SER A 66 25.167 72.543 14.614 1.00108.68
ATOM 465 CB SER A 66 24.507 71.186 14.365 1.00131.29
ATOM 466 OG SER A 66 25.451 70.136 14.446 1.00132.76
ATOM 467 C SER A 66 24.184 73.648 14.239 1.00107.83
ATOM 468 0 SER A 66 24.159 74.706 14.859 1.00109.21
ATOM 469 N THR A 67 23.368 73.384 13.223 1.00 37.78
ATOM 470 CA THR A 67 22.399 74.358 12.731 1.00 37.55
ATOM 471 CB THR A 67 23.084 75.605 12.161 1.00 38.25
ATOM 472 OG1 THR A 67 22.154 76.305 11.322 1.00 36.51
ATOM 473 CG2 THR A 67 24.319 75.214 11.337 1.00 36.90
ATOM 474 C THR A 67 21.540 73.785 11.614 1.00 37.84
ATOM 475 O THR A 67 21.777 74.052 10.435 1.00 38.15
ATOM 476 N ILE A 68 20.535 73.011 12.005 1.00 72.99
ATOM 477 CA ILE A 68 19.602 72.365 11.088 1.00 74.27
ATOM 478 CB ILE A 68 18.499 71.679 11.878 1.00 43.22
ATOM 479 CG2 ILE A 68 17.632 70.891 10.946 1.00 43.12
ATOM 480 CG1 ILE A 68 19.109 70.788 12.957 1.00 42.80
ATOM 481 CD1 ILE A 68 18.125 70.363 14.024 1.00 42.11
ATOM 482 C ILE A 68 18.915 73.306 10.093 1.00 74.17
ATOM 483 0 ILE A 68 17.998 74.036 10.470 1.00 75.51
ATOM 484 N PRO A 69 19.335 73.293 8.808 1.00 47.83
ATOM 485 CD PRO A 69 20.552 72.652 8.266 1.00 30.92
ATOM 486 CA PRO A 69 18.723 74.161 7.790 1.00 48.16
ATOM 487 CB PRO A 69 19.365 73.676 6.497 1.00 30.11
ATOM 488 CG PRO A 69 20.781 73.419 6.965 1.00 30.52
ATOM 489 C PRO A 69 17.204 74.102 7.739 1.00 46.97
ATOM 490 0 PRO A 69 16.616 73.020 7.661 1.00 46.45
ATOM 491 N GLY A 70 16.586 75.283 7.799 1.00 82.64
ATOM 492 CA GLY A 70 15.138 75.393 7.753 1.00 83.42
ATOM 493 C GLY A 70 14.471 75.570 9.106 1.00 83.11
ATOM 494 0 GLY A 70 13.249 75.722 9.188 1.00 83.49
ATOM 495 N VAL A 71 15.266 75.548 10.170 1.00 25.92
9

CA 02440409 2003-09-09
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ATOM 496 CA VAL A 71 14.738 75.700 11.519 1.00 25.88
ATOM 497 CB VAL A 71 15.182 74.528 12.462 1.00 69.23
ATOM 498 CG1 VAL A 71 14.438 74.612 13.787 1.00 69.55
ATOM 499 CG2 VAL A 71 14.923 73.176 11.811 1.00 69.40
ATOM 500 C VAL A 71 15.254 76.995 12.109 1.00 24.57
ATOM 501 0 VAL A 71 16.385 77.396 11.857 1.00 23.69
ATOM 502 N LYS A 72 14.422 77.642 12.910 1.00 61.33
ATOM 503 CA LYS A 72 14.794 78.891 13.559 1.00 61.86
ATOM 504 CB LYS A 72 13.567 79.514 14.227 1.00 80.38
ATOM 505 CG LYS A 72 13.835 80.872 14.830 1.00 81.39
ATOM 506 CD LYS A 72 12.590 81.460 15.447 1.00 82.45
ATOM 507 CE LYS A 72 12.867 82.860 15.951 1.00 83.55
ATOM 508 NZ LYS A 72 11.769 83.344 16.827 1.00 85.83
ATOM 509 C LYS A 72 15.885 78.689 14.601 1.00 61.01
ATOM 510 O LYS A 72 16.965 79.264 14.503 1.00 60.86
ATOM 511 N GLU A 73 15.580 77.860 15.593 1.00 37.71
ATOM 512 CA GLU A 73 16.475 77.557 16.702 1.00 38.31
ATOM 513 CB GLU A 73 15.911 76.368 17.489 1.00 60.62
ATOM 514 CG GLU A 73 14.399 76.363 17.551 1.00 63.75
ATOM 515 CD GLU A 73 13.844 77.717 17.925 1.00 65.67
ATOM 516 OE1 GLU A 73 13.822 78.040 19.130 1.00 66.19
ATOM 517 OE2 GLU A 73 13.444 78.467 17.009 1.00 67.76
ATOM 518 C GLU A 73 17.945 77.287 16.367 1.00 37.99
ATOM 519 O GLU A 73 18.520 77.864 15.445 1.00 38.72
ATOM 520 N ASP A 74 18.534 76.388 17.151 1.00 82.96
ATOM 521 CA ASP A 74 19.935 75.994 17.037 1.00 81.66
ATOM 522 CB ASP A 74 20.808 77.116 17.581 1.00 54.13
ATOM 523 CG ASP A 74 22.192 76.656 17.930 1.00 52.60
ATOM 524 OD1 ASP A 74 22.333 75.533 18.467 1.00 50.96
ATOM 525 OD2 ASP A 74 23.137 77.433 17.683 1.00 52.36
ATOM 526 C ASP A 74 20.085 74.747 17.903 1.00 80.79
ATOM 527 0 ASP A 74 19.780 74.783 19.090 1.00 81.18
ATOM 528 N VAL A 75 20.584 73.661 17.326 1.00 40.61
ATOM 529 CA VAL A 75 20.710 72.400 18.055 1.00 38.44
ATOM 530 CB VAL A 75 21.747 71.469 17.412 1.00 21.23
ATOM 531 CG1 VAL A 75 21.643 70.059 18.019 1.00 17.92
ATOM 532 CG2 VAL A 75 21.539 71.446 15.904 1.00 20.66
ATOM 533 C VAL A 75 21.040 72.538 19.530 1.00 38.36
ATOM 534 0 VAL A 75 20.438 71.853 20.362 1.00 38.29
ATOM 535 N VAL A 76 21.987 73.411 19.864 1.00 74.82
ATOM 536 CA VAL A 76 22.347 73.584 21.267 1.00 75.22
ATOM 537 CB VAL A 76 23.393 74.690 21.484 1.00 34.21
ATOM 538 CG1 VAL A 76 23.993 74.554 22.876 1.00 34.49
ATOM 539 CG2 VAL A 76 24.480 74.592 20.448 1.00 34.06
ATOM 540 C VAL A 76 21.067 73.977 21.967 1.00 76.36
ATOM 541 O VAL A 76 20.603 73.271 22.866 1.00 76.45
ATOM 542 N GLU A 77 20.494 75.098 21.534 1.00 77.76
ATOM 543 CA GLU A 77 19.235 75.587 22.082 1.00 76.31
ATOM 544 CB GLU A~ 77 18.611 76.604 21.133 1.00 63.33
ATOM 545 CG GLU A 77 18.347 77.953 21.750 1.00 64.17
ATOM 546 CD GLU A 77 17.173 78.659 21.102 1.00 64.78
ATOM 547 OE1 GLU A 77 16.026 78.202 21.309 1.00 64.71
ATOM 548 OE2 GLU A 77 17.394 79.660 20.387 1.00 65.41
ATOM 549 C GLU A 77 18.295 74.393 22.206 1.00 75.12
ATOM 550 0 GLU A 77 17.823 74.062 23.293 1.00 75.46
ATOM 551 N ILE A 78 18.038 73.755 21.066 1.00 32.87

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 552 CA ILE A 78 17.176 72.583 20.982 1.00 30.59
6
ATOM 553 CB ILE A 78 17.406 71.828 19.661 1.00 32.78
6
ATOM 554 CG2 ILE A 78 16.570 70.569 19.627 1.00 31.04
6
ATOM 555 CG1 ILE A 78 17.049 72.743 18.482 1.00 33.50
6
ATOM 556 CD1 ILE A 78 17.468 72.214 17.114 1.00 34.08
6
ATOM 557 C ILE A 78 17.462 71.645 22.136 1.00 30.32
6
ATOM 558 0 ILE A 78 16.544 71.190 22.818 1.00 29.37
8
ATOM 559 N ILE A 79 18.737 71.361 22.362 1.00 37.89
7
ATOM 560 CA ILE A 79 19.094 70.471 23.441 1.00 39.91
6
ATOM 561 CB ILE A 79 20.512 69.954 23.281 1.00 45.66
6
ATOM 562 CG2 ILE A 79 20.957 69.240 24.534 1.00 45.36
6
ATOM 563 CG1 ILE A 79 20.561 68.981 22.113 1.00 46.47
6
ATOM 564 CD1 ILE A 79 21.890 68.301 21.967 1.00 48.56
6
ATOM 565 C ILE A 79 18.951 71.160 24.784 1.00 41.42
6
ATOM 566 0 ILE A 79 18.754 70.499 25.810 1.00 42.07
8
ATOM 567 N LEU A 80 19.050 72.488 24.786 1.00 89.16
7
ATOM 568 CA LEU A 80 18.912 73.250 26.023 1.00 89.46
6
ATOM 569 CB LEU A 80 19.462 74.678 25.856 1.00 35.09
6
ATOM 570 CG LEU A 80 20.982 74.838 26.063 1.00 34.34
6
ATOM 571 CD1 LEU A 80 21.423 76.281 25.845 1.00 34.07
6
ATOM 572 CD2 LEU A 80 21.331 74.392 27.482 1.00 33.85
6
ATOM 573 C LEU A 80 17.447 73.279 26.425 1.00 89.37
6
ATOM 574 0 LEU A 80 17.120 73.370 27.609 1.00 90.96
8
ATOM 575 N ASN A 81 16.569 73.189 25.428 1.00 41.89
7
ATOM 576 CA ASN A 81~ 15.134 73.170 25.674 1.00 41.74
6
ATOM 577 CB ASN A 81 14.362 73.564 24.412 1.00 69.15
6
ATOM 578 CG ASN A 81 14.578 75.019 24.024 1.00 70.45
6
ATOM 579 OD1 ASN A 81 15.661 75.406 23.595 1.00 70.24
8
ATOM 580 ND2 ASN A 81 13.542 75.833 24.180 1.00 71.82
7
ATOM 581 C ASN A 81 14.731 71.766 26.125 1.00 40.96
6
ATOM 582 0 ASN A 81 14.149 71.601 27.200 1.00 40.05
8
ATOM 583 N LEU A 82 15.053 70.755 25.316 1.00 63.42
7
ATOM 584 CA LEU A 82 14.724 69.372 25.667 1.00 64.05
6
ATOM 585 CB LEU A 82 15.370 68.366 24.700 1.00 58.81
6
ATOM 586 CG LEU A 82 14.932 68.301 23.231 1.00 59.30
6
ATOM 587 CD1 LEU A 82 15.628 67.112 22.568 1.00 58.70
6
ATOM 588 CD2 LEU A 82 13.411 68.159 23.123 1.00 59.58
6
ATOM 589 C LEU A 82 15.200 69.062 27.074 1.00 64.25
6
ATOM 590 0 LEU A 82 14.629 68.233 27.766 1.00 64.54
8
ATOM 591 N LYS A 83 16.255 69.730 27.504 1.00 93.29
7
ATOM 592 CA LYS A 83 16.771 69.490 28.835 1.00 95.39
6
ATOM 593 CB LYS A 83 17.951 70.441 29.100 1.00 57.62
6
ATOM 594 CG LYS A 83 18.613 70.272 30.464 1.00 58.61
6
ATOM 595 CD LYS A 83 19.963 70.983 30.554 1.00 58.45
6
ATOM 596 CE LYS A 83 21.016 70.259 29.735 1.00 58.82
6
ATOM 597 NZ LYS A 83 22.365 70.853 29.907 1.00 57.41
7
ATOM 598 C LYS A 83 15.651 69.675 29.871 1.00 96.28
6
ATOM 599 0 LYS A 83 15.738 69.164 30.990 1.00 97.41
8
ATOM 600 N GLU A 84 14.583 70.368 29.472 1.00 83.14
7
ATOM 601 CA GLU A 84 13.446 70.653 30.354 1.00 83.07
6
ATOM 602 CB GLU A 84 13.032 72.108 30.185 1.00 62.64
6
ATOM 603 CG GLU A 84 14.028 73.098 30.716 1.00 63.41
6
ATOM 604 CD GLU A 84 13.747 74.495 30.214 1.00 65.27
6
ATOM 605 OE1 GLU A 84 12.552 74.877 30.155 1.00 66.33
8
ATOM 606 OE2 GLU A 84 14.719 75.216 29.883 1.00 66.86
8
ATOM 607 C GLU A 84 12.200 69.779 30.191 1.00 82.17
6
11

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 608 0 GLU A 84 11.248 69.902 30.957 1.00 82.78
ATOM 609 N LEU A 85 12.199 68.913 29.189 1.00 32.42
ATOM 610 CA LEU A 85 11.065 68.033 28.929 1.00 30.61
ATOM 611 CB LEU A 85 11.322 67.244 27.649 1.00 29.83
ATOM 612 CG LEU A 85 10.253 66.272 27.182 1.00 28.15
ATOM 613 CD1 LEU A 85 9.219 67.034 26.338 1.00 27.30
ATOM 614 CD2 LEU A 85 10.921 65.138 26.386 1.00 27.81
ATOM 615 C LEU A 85 10.857 67.065 30.086 1.00 30.06
ATOM 616 0 LEU A 85 11.779 66.380 30.499 1.00 28.59
ATOM 617 N VAL A 86 9.645 67.007 30.617 1.00 42.85
ATOM 618 CA VAL A 86 9.384 66.093 31.716 1.00 43.95
ATOM 619 CB VAL A 86 8.760 66.804 32.932 1.00 39.00
ATOM 620 CG1 VAL A 86 7.557 67.620 32.527 1.00 38.89
ATOM 621 CG2 VAL A 86 8.368 65.766 33.960 1.00 40.01
ATOM 622 C VAL A 86 8.462 64.972 31.298 1.00 43.36
ATOM 623 0 VAL A 86 7.309 65.215 30.938 1.00 43.19
ATOM 624 N VAL A 87 8.966 63.743 31.351 1.00 21.15
ATOM 625 CA VAL A 87 8.154 62.603 30.958 1.00 23.38
ATOM 626 CB VAL A 87 8.903 61.625 30.004 1.00 39.94
ATOM 627 CG1 VAL A 87 9.272 62.330 28.711 1.00 40.80
ATOM 628 CG2 VAL A 87 10.139 61.075 30.676 1.00 42.12
ATOM 629 C VAL A 87 7.702 61.822 32.157 1.00 24.21
ATOM 630 0 VAL A 87 8.396 61.768 33.169 1.00 23.51
ATOM 631 N ARG A 88 6.512 61.243 32.026 1.00 76.51
ATOM 632 CA ARG A 88 5.903 60.411 33.049 1.00 80.48
ATOM 633 CB ARG A 88 4.408 60.688 33.132 1.00 63.66
ATOM 634 CG ARG A 88 3.694 59.846 34.164 1.00 64.46
ATOM 635 CD ARG A 88 2.414 60.503 34.634 1.00 65.19
ATOM 636 NE ARG A 88 1.763 59.729 35.683 1.00 66.10
ATOM 637 CZ ARG A 88 0.793 60.196 36.463 1.00 68.02
ATOM 638 NH1 ARG A 88 0.259 59.417 37.392 1.00 69.40
ATOM 639 NH2 ARG A 88 0.356 61.443 36.322 1.00 68.70
ATOM 640 C ARG A 88 6.142 59.014 32.517 1.00 82.14
ATOM 641 O ARG A 88 6.387 58.861 31.326 1.00 83.19
ATOM 642 N PHE A 89 6.073 57.991 33.362 1.00 58.23
ATOM 643 CA PHE A 89 6.326 56.644 32.862 1.00 61.38
ATOM 644 CB PHE A 89 7.625 56.125 33.483 1.00 54.32
ATOM 645 CG PHE A 89 8.849 56.458 32.668 1.00 52.53
ATOM 646 CD1 PHE A 89 8.843 57.539 31.788 1.00 51.76
ATOM 647 CD2 PHE A 89 9.994 55.672 32.752 1.00 51.86
ATOM 648 CE1 PHE A 89 9.953 57.831 31.009 1.00 50.89
ATOM 649 CE2 PHE A 89 11.114 55.956 31.976 1.00 50.55
ATOM 650 CZ PHE A 89 11.091 57.037 31.097 1.00 50.23
ATOM 651 C PHE A 89 5.210 55.595 32.952 1.00 63.31
ATOM 652 0 PHE A 89 5.355 54.491 32.432 1.00 64.6'7
ATOM 653 N LEU A 90 4.097 55.938 33.591 1.00 77.73
ATOM 654 CA LEU A 90 2.952 55.033 33.721 1.00 80.08
ATOM 655 CB LEU A 90 2.105 55.065 32.447 1.00 30.07
ATOM 656 CG LEU A 90 1.734 56.412 31.834 1.00 29.09
ATOM 657 CD1 LEU A 90 0.710 56.165 30.757 1.00 28.59
ATOM 658 CD2 LEU A 90 1.149 57.351 32.870 1.00 29.27
ATOM 659 C LEU A 90 3.250 53.569 34.042 1.00 81.65
ATOM 660 0 LEU A 90 2.348 52.738 33.956 1.00 82.47
ATOM 661 N ASP A 91 4.492 53.244 34.396 1.00108.42
ATOM 662 CA ASP A 91 4.856 51.862 34.719 1.00111.01
ATOM 663 CB ASP A 91 4.874 50.999 33.471 1.00109.60
12

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 664 CG ASP A 91 6.246 50.931 32.855 1.00111.18
6
ATOM 665 OD1 ASP A 91 6.576 51.826 32.056 1.00111.61
.8
ATOM 666 OD2 ASP A 91 7.003 49.995 33.190 1.00112.73
8
ATOM 667 C ASP A 91 6.240 51.764 35.345 1.00111.70
6
ATOM 668 0 ASP A 91 6.993 52.736 35.366 1.00112.36
8
ATOM 669 N PRO A 92 6.596 50.577 35.856 1.00 72.17
7
ATOM 670 CD PRO A 92 5.630 49.603 36.396 1.00138.10
6
ATOM 671 CA PRO A 92 7.910 50.389 36.475 1.00 72.33
6
ATOM 672 CB PRO A 92 7.560 49.702 37.781 1.00138.16
6
ATOM 673 CG PRO A 92 6.484 48.769 37.341 1.00138.73
6
ATOM 674 C PRO A 92 8.902 49.564 35.655 1.00 71.96
6
ATOM 675 O PRO A 92 8.517 48.719 34.844 1.00 71.56
8
ATOM 676 N ALA A 93 10.186 49.841 35.874 1.00162.67
7
ATOM 677 CA ALA A 93 11.286 49.132 35.225 1.00162.90
6
ATOM 678 CB ALA A 93 11.106 47.636 35.429 1.00114.11
6
ATOM 679 C ALA A 93 11.551 49.407 33.747 1.00162.76
6
ATOM 680 O ALA A 93 11.126 48.620 32.900 1.00162.55
8
ATOM 681 N TRP A 94 12.270 50.493 33.442 1.00 96.59
7
ATOM 682 CA TRP A 94 12.607 50.826 32.055 1.00 95.32
6
ATOM 683 CB TRP A 94 11.431 50.488 31.137 1.00107.02
6
ATOM 684 CG TRP A 94 10.337 51.499 31.193 1.00108.85
6
ATOM 685 CD2 TRP A 94 9.655 52.086 30.083 1.00110.00
6
ATOM 686 CE2 TRP A 94 8.717 52.997 30.607 1.00111.39
6
ATOM 687 CE3 TRP A 94 9.745 51.932 28.695 1.00110.71
6
ATOM 688 CD1 TRP A 94 9.800 52.057 32.312 1.00110.15
6
ATOM 689 NE1 TRP A 94 8.829 52.959 31.971 1.00111.87
7
ATOM 690 CZ2 TRP A 94 7.871 53.756 29.792 1.00112.08
6
ATOM 691 CZ3 TRP A 94 8.902 52.687 27.885 1.00112.05
6
ATOM 692 CH2 TRP A 94 7.979 53.587 28.438 1.00112.66
6
ATOM 693 C TRP A 94 13.012 52.272 31.763 1.00 92.85
6
ATOM 694 O TRP A 94 12.934 53.149 32.623 1.00 92.99
8
ATOM 695 N ARG A 95 13.433 52.482 30.514 1.00 77.36
7
ATOM 696 CA ARG A 95 13.819 53.780 29.951 1.00 73.49
6
ATOM 697 CB ARG A 95 15.327 53.988 30.002 1.00 89.27
6
ATOM 698 CG ARG A 95 16.061 53.578 28.730 1.00 91.22
6
ATOM 699 CD ARG A 95 16.014 52.068 28.466 1.00 92.63
6
ATOM 700 NE ARG A 95 16.719 51.720 27.231 1.00 94.64
7
ATOM 701 CZ ARG A 95 16.777 50.500 26.706 1.00 96.74
6
ATOM 702 NH1 ARG A 95 16.164 49.483 27.305 1.00 97.64
7
ATOM 703 NH2 ARG A 95 17.452 50.299 25.579 1.00 98.03
7
ATOM 704 C ARG A 95 13.379 53.654 28.493 1.00 69.86
6
ATOM 705 O ARG A 95 13.380 52.562 27.946 1.00 70.69
8
ATOM 706 N THR A 96 13.012 54.745 27.846 1.00 30.81
7
ATOM 707 CA THR A 96 12.552 54.628 26.469 1.00 26.69
~ 6
ATOM 708 CB THR A 96 10.978 54.571 26.415 1.00 13.87
6
ATOM 709 OG1 THR A 96 10.537 54.617 25.053 1.00 13.87
8
ATOM 710 CG2 THR A 96 10.353 55.715 27.192 1.00 13.87
6
ATOM 711 C THR A 96 13.093 55.728 25.560 1.00 24.46
6
ATOM 712 0 THR A 96 13.990 56.467 25.967 1.00 23.10
8
ATOM 713 N THR A 97 12.554 55.839 24.342 1.00 70.32
7
ATOM 714 CA THR A 97 13.029 56.845 23.400 1.00 68.74
6
ATOM 715 CB THR A 97 14.070 56.273 22.467 1.00 66.34
6
ATOM 716 OG1 THR A 97 14.815 55.258 23.147 1.00 67.45
8
ATOM 717 CG2 THR A 97 15.001 57.381 21.986 1.00 65.31
6
ATOM 718 C THR A 97 11.998 57.494 22.497 1.00 67.24
6
ATOM 719 0 THR A 97 11.613 56.927 21.478 1.00 68.97
8
13

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 720 N LEU A 98 11.589 58.704 22.856 1.00 38.90
7
ATOM 721 CA LEU A 98 10.628 59.478 22.079 1.00 36.55
6
ATOM 722 CB LEU A 98 10.416 60.843 22.750 1.00 32.16
6
ATOM 723 CG LEU A 9.8 9.636 60.817 24.077 1.00 31.78
6
ATOM 724 CD1 LEU A 98 8.132 60.750 23.797 1.00 31.19
6
ATOM 725 CD2 LEU A 98 10.073 59.627 24.931 1.00 30.42
6
ATOM 726 C LEU A 98 11.131 59.647 20.638 1.00 35.43
6
ATOM 727 0 LEU A 98 12.309 59.422 20.348 1.00 37.05
8
ATOM 728 N ILE A 99 10.220 60.023 19.746 1.00 13.87
7
ATOM 729 CA ILE A 99 10.493 60.208 18.322 1.00 13.87
6
ATOM 730 CB ILE A 99 9.951 59.037 17.487 1.00 28.58
6
ATOM 731 CG2 ILE A 99 10.336 59.220 16.058 1.00 28.83
6
ATOM 732 CG1 ILE A 99 10.455 57.698 18.045 1.00 29.22
6
ATOM 733 CD1 ILE A 99 11.967 57.497 18.012 1.00 29.54
6
ATOM 734 C ILE A 99 9.749 61.451 17.892 1.00 13.87
6
ATOM 735 0 ILE A 99 8.802 61.856 18.546 1.00 13.87
8
ATOM 736 N LEU A 100 10.149 62.063 16.793 1.00 15.12
7
ATOM 737 CA LEU A 100 9.451 63.261 16.368 1.00 18.83
6
ATOM 738 CB LEU A 100 9.689 64.400 17.362 1.00 24.33
6
ATOM 739 CG LEU A 100 9.854 65.811 16.787 1.00 24.43
6
ATOM 740 CD1 LEU A 100 8.642 66.171 16.002 1.00 26.73
6
ATOM 741 CD2 LEU ,A100 10.055 66.820 17.892 1.00 23.82
6
ATOM 742 C LEU A 100 9.858 63.709 14.994 1.00 19.65
6
ATOM 743 O LEU A 100 11.032 63.946 14.734 1.00 19.44
8
ATOM 744 N ARG A 101 8.858 63.847 14.133 1.00 55.57
7
ATOM 745 CA ARG A 101 9.036 64.286 12.757 1.00 58.59
6
ATOM 746 CB ARG A 101 8.513 63.191 11.815 1.00132.06
6
ATOM 747 CG ARG A 101 9.239 63.046 10.478 1.00135.60
6
ATOM 748 CD ARG A 101 8.651 61.883 9.672 1.00137.39
6
ATOM 749 NE ARG A 101 9.392 61.598 8.443 1.00139.28
7
ATOM 750 CZ ARG A 101 9.039 60.669 7.556 1.00140.18
6
ATOM 751 NH1 ARG A 101 7.953 59.931 7.756 1.00139.80
7
ATOM 752 NH2 ARG A 101 9.777 60.467 6.472 1.00140.56
7
ATOM 753 C ARG A 101 8.192 65.564 12.623 1.00 58.89
6
ATOM 754 0 ARG A 101 7.064 65.626 13.129 1.00 59.23
8
ATOM 755 N ALA A 102 8.720 66.587 11.961 1.00 35.89
7
ATOM 756 CA ALA A 102 7.947 67.821 11.811 1.00 38.61
6
ATOM 757 CB ALA A 102 8.293 68.793 12.932 1.00 67.58
6
ATOM 758 C ALA A 102 8.137 68.493 10.451 1.00 38.78
6
ATOM 759 0 ALA A 102 9.262 68.718 10.003 1.00 38.84
8
ATOM 760 N GLU A 103 7.030 68.835 ~ 9.806 1.00 34.50
7
ATOM 761 CA GLU A 103 7.088 69.445 8.488 1.00 36.32
6
ATOM 762 CB GLU A 103 5.869 69.029 7.673 1.00 66.19
6
ATOM 763 CG GLU A 103 5.987 67.636 7.113 1.00 66.55
6
ATOM 764 CD GLU A 103 7.340 67.422 6.499 1.00 65.97
6
ATOM 765 OE1 GLU A 103 7.770 68.300 5.719 1.00 64.19
8
ATOM 766 OE2 GLU A 103 7.973 66.387 6.802 1.00 66.71
8
ATOM 767 C GLU A 103 7.221 70.952 8.435 1.00 38.51
6
ATOM 768 O GLU A 103 7.263 71.638 9.456 1.00 38.40
8
ATOM 769 N GLY A 104 7.280 71.444 7.202 1.00 82.92
7
ATOM 770 CA GLY A 104 7.412 72.859 6.906 1.00 86.20
6
ATOM 771 C GLY A 104 7.259 73.885 8.006 1.00 87.20
6
ATOM 772 0 GLY A 104 8.069 73.938 8.927 1.00 87.75
8
ATOM 773 N PRO A 105 6.220 74.729 7.925 1.00121.54
7
ATOM 774 CD PRO A 105 5.240 74.798 6.823 1.00 82.59
6
ATOM 775 CA PRO A 105 5.956 75.775 8.911 1.00122.33
6
14

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 776 CB PRO A 105 5.165 76.789 8.101 1.00 82.71
ATOM 777 CG PRO A 105 4.280 75.890 7.292 1.00 82.69
ATOM 778 C PRO A 105 5.187 75.310 10.143 1.00121.71
ATOM 779 O PRO A 105 4.319 74.442 10.050 1.00121.21
ATOM 780 N LYS A 106 5.525 75.908 11.284 1.00 81.15
ATOM 781 CA LYS A 106 4.887 75.646 12.572 1.00 81.76
ATOM 782 CB LYS A 106 4.137 74.322 12.567 1.00 80.86
ATOM 783 CG LYS A 106 5.010 73.105 12.524 1.00 80.61
ATOM 784 CD LYS A 106 4.113 71.913 12.641 1.00 82.64
ATOM 785 CE LYS A 106 4.884 70.632 12.761 1.00 84.29
ATOM 786 NZ LYS A 106 3.925 69.490 12.835 1.00 86.22
ATOM 787 C LYS A 106 5.885 75.632 13.711 1.00 81.50
ATOM 788 O LYS A 106 7.014 76.092 13.565 1.00 81.66
ATOM 789 N GLU A 107 5.447 75.108 14.849 1.00 61.87
ATOM 790 CA GLU A 107 6.283 75.001 16.035 1.00 62.76
ATOM 791 CB GLU A 107 5.757 75.913 17.136 1.00 63.05
ATOM 792 CG GLU A 107 5.992 77.370 16.866 1.00 62.98
ATOM 793 CD GLU A 107 5.557 78.241 18.015 1.00 63.40
ATOM 794 OE1 GLU A 107 5.837 77.874 19.181 1.00 62.44
ATOM 795 OE2 GLU A 107 4.941 79.295 17.743 1.00 64.68
ATOM 796 C GLU A 107 6.275 73.563 16.524 1.00 62.81
ATOM 797 O GLU A 107 5.216 72.949 16.628 1.00 64.05
ATOM 798 N VAL A 108 7.445 73.015 16.828 1.00 92.35
ATOM 799 CA VAL A 108 7.489 71.641 17.298 1.00 90.77
ATOM 800 CB VAL A 108 8.786 70.926 16.886 1.00 24.79
ATOM 801 CG1 VAL A 108 8.524 69.456 16.759 1.00 22.72
ATOM 802 CG2 VAL A 108 9.305 71.473 15.599 1.00 23.64
ATOM 803 C VAL A 108 7.373 71.569 18.813 1.00 90.99
ATOM 804 O VAL A 108 8.067 70.785 19.448 1.00 91.87
ATOM 805 N ARG A 109 6.504 72.389 19.394 1.00 16.19
ATOM 806 CA ARG A 109 6.318 72.367 20.836 1.00 17.08
ATOM 807 CB ARG A 109 5.075 73.192 21.230 1.00 79.46
ATOM 808 CG ARG A 109 3.702 72.618 20.870 1.00 82.11
ATOM 809 CD ARG A 109 3.528 72.443 19.373 1.00 82.88
ATOM 810 NE ARG A 109 2.130 72.272 18.982 1.00 82.78
ATOM 811 CZ ARG A 109 1.738 71.912 17.762 1.00 83.25
ATOM 812 NH1 ARG A 109 2.636 71.675 16.816 1.00 82.30
ATOM 813 NH2 ARG A 109 0.448 71.812 17.477 1.00 83.70
ATOM 814 C ARG A 109 6.211 70.916 21.329 1.00 16.63
ATOM 815 0 ARG A 109 5.687 70.052 20.636 1.00 16.88
ATOM 816 N ALA A 110 6.733 70.653 22.520 1.00 43.26
ATOM 817 CA ALA A 110 6.740 69.314 23.119 1.00 44.15
ATOM 818 CB ALA A 110 6.592 69.434 24.630 1.00 62.64
ATOM 819 C ALA A 110 5.731 68.300 22.582 1.00 44.52
ATOM 820 O ALA A 110 6.111 67.264 22.031 1.00 43.73
ATOM 821 N VAL A 111 4.450 68.589 22.757 1.00 59.09
ATOM 822 CA VAL A 111 3.391 67.687 22.302 1.00 60.52
ATOM 823 CB VAL A 111 2.128 68.466 21.894 1.00208.87
ATOM 824 CG1 VAL A 111 1.013 67.493 21.525 1.00208.87
ATOM 825 CG2 VAL A 111 1.698 69.385 23.021 1.00208.87
ATOM 826 C VAL A 111 3.792 66.821 21.119 1.00 58.77
ATOM 827 O VAL A 111 3.854 65.596 21.229 1.00 58.31
ATOM 828 N ASP A 112 4.046 67.490 19.996 1.00 43.13
ATOM 829 CA ASP A 112 4.447 66.875 18.733 1.00 43.00
ATOM 830 CB ASP A 112 5.244 67.892 17.922 1.00 56.89
ATOM 831 CG ASP A 112 4.385 69.026 17.420 1.00 56.48

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 832 OD1 ASP A 112 3.586 68.794 16.497 1.00 55.87
ATOM 833 OD2 ASP A 112 4.496 70.145 17.948 1.00 56.33
ATOM 834 C ASP A 112 5.225 65.556 18.789 1.00 42.03
ATOM 835 O ASP A 112 5.499 64.949 17.753 1.00 41.37
ATOM 836 N PHE A 113 5.596 65.113 19.983 1.00 66.40
ATOM 837 CA PHE A 113 6.326 63.861 20.106 1.00 67.18
ATOM 838 CB PHE A 113 6.863 63.705 21.527 1.00 48.77
ATOM 839 CG PHE A 113 8.243 64.271 21.710 1.00 45.55
ATOM 840 CD1 PHE A 113 8.769 65.166 20.786 1.00 43.11
ATOM 841 CD2 PHE A 113 9.018 63.901 22.798 1.00 44.09
ATOM 842 CE1 PHE A 113 10.036 65.672 20.940 1.00 42.90
ATOM 843 CE2 PHE A 113 10.285 64.405 22.959 1.00 43.06
ATOM 844 CZ PHE A 113 10.797 65.294 22.027 1.00 43.40
ATOM 845 C PHE A 113 5.447 62.681 19.734 1.00 67.93
ATOM 846 0 PHE A 113 4.225 62.817 19.655 1.00 68.13
ATOM 847 N THR A 114 6.077 61.532 19.500 1.00 87.75
ATOM 848 CA THR A 114 5.366 60.317 19.122 1.00 87.95
ATOM 849 CB THR A 114 6.351 59.177 18.778 1.00 77.17
ATOM 850 OG1 THR A 114 5.628 58.046 18.287 1.00 75.93
ATOM 851 CG2 THR A 114 7.132 58.753 20.007 1.00 78.13
ATOM 852 C THR A 114 4.433 59.833 20.226 1.00 88.39
ATOM 853 O THR A 114 4.879 59.372 21.278 1.00 88.57
ATOM 854 N PRO A 115 3.115 59.943 20.005 1.00 98.73
ATOM 855 CD PRO A 115 2.382 60.327 18.785 1.00 90.85
ATOM 856 CA PRO A 115 2.203 59.480 21.053 1.00 99.15
ATOM 857 CB PRO A 115 0.824 59.750 20.445 1.00 90.46
ATOM 858 CG PRO A 115 1.071 59.620 18.964 1.00 91.10
ATOM 859 C PRO A 115 2.491 58.003 21.267 1.00 98.39
ATOM 860 O PRO A 115 2.155 57.190 20.415 1.00 98.70
ATOM 861 N SER A 116 3.117 57.640 22.383 1.00 43.20
ATOM 862 CA SER A 116 3.438 56.228 22.566 1.00 43.88
ATOM 863 CB SER A 116 4.600 55.863 21.632 1.00127.33
ATOM 864 OG SER A 116 5.700 56.740 21.807 1.00127.37
ATOM 865 C SER A 116 3.724 55.633 23.955 1.00 44.19
ATOM 866 O SER A 116 4.506 56.168 24.747 1.00 43.78
ATOM 867 N ALA A 117 3.087 54.490 24.210 1.00137.91
ATOM 868 CA ALA A 117 3.233 53.735 25.450 1.00139.42
ATOM 869 CB ALA A 117 4.570 53.008 25.448 1.00 13.87
ATOM 870 C ALA A 117 3.081 54.534 26.740 1.00139.97
ATOM 871 0 ALA A 117 2.550 55.642 26.745 1.00139.94
ATOM 872 N ASP A 118 3.551 53.941 27.834 1.00 85.71
ATOM 873 CA ASP A 118 3.482 54.546 29.160 1.00 87.10
ATOM 874 CB ASP A 118 3.999. 53.559 30.207 1.00196.72
ATOM 875 CG ASP A 118 3.155 52.311 30.302 1.00198.95
ATOM 876 OD1 ASP A 118 1.950 52.431 30.601 1.00200.67
ATOM 877 OD2 ASP A 118 3.697 51.207 30.083 1.00199.32
ATOM 878 C ASP A 118 4.304 55.822 29.238 1.00 86.97
ATOM 879 O ASP A 118 5.182 55.943 30.087 1.00 87.04
ATOM 880 N VAL A 119 4.007 56.779 28.368 1.00144.02
ATOM 881 CA VAL A 119 4.756 58.026 28.339 1.00143.57
ATOM 882 CB VAL A 119 5.696 58.055 27.106 1.00139.37
ATOM 883 CG1 VAL A 119 6.478 59.356 27.069 1.00140.66
ATOM 884 CG2 VAL A 119 6.640 56.864 27.142 1.00139.26
ATOM 885 C VAL A 119 3.869 59.268 28.297 1.00142.87
ATOM 886 0 VAL A 119 3.183 59.505 27.301 1.00143.40
ATOM 887 N GLU A 120 3.882 60.060 29.370 1.00 58.92
16

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 888 CA GLU A 120 3.087 61.290 29.402 1.00 57.67
6
ATOM 889 CB GLU A 120 2.308 61.423 30.708 1.00 57.66
6
ATOM 890 CG GLU A 120 1.164 60.461 30.889 1.00 55.40
6
ATOM 891 CD GLU A 120 0.288 60.853 32.059 1.00 52.98
6
ATOM 892 OE1 GLU A 120 -0.443 61.863- 31.938 1.00 52.12
8
ATOM 893 OE2 GLU A 120 0.333 60.160 33.096 1.00 50.64
8
ATOM 894 C GLU A 120 3.947 62.538 29.255 1.00 56.66
6
ATOM 895 O GLU A 120 4.692 62.886 30.169 1.00 57.46
8
ATOM 896 N ILE A 121 3.850 63.209 28.111 1.00 43.45
7
ATOM 897 CA ILE A 121 4.604 64.440 27.898 1.00 42.84
6
ATOM 898 CB ILE A 121 4.522 64.902 26.425 1.00 53.47
6
ATOM 899 CG2 ILE A 121 5.189 66.249 26.242 1.00 53.52
6
ATOM 900 CG1 ILE A 121 5.215 63.875 25.537 1.00 53.84
6
ATOM 901 CD1 ILE A 121 6.623 63.569 25.968 1.00 54.20
6
ATOM 902 C ILE A 121 3.967 65.470 28.830 1.00 41.89
6
ATOM 903 O ILE A 121 3.431 66.515 28.406 1.00 41.93
8
ATOM 904 N MET A 122 4.020 65.113 30.112 1.00 48.93
7
ATOM 905 CA MET A 122 3.494 65.888 31.214 1.00 48.79
6
ATOM 906 CB MET A 122 4.374 65.665 32.436 1.00 96.83
6
ATOM 907 CG MET A 122 3.626 65.452 33.729 1.00 99.46
6
ATOM 908 SD MET A 122 3.284 63.721 34.020 1.00100.08
16
ATOM 909 CE MET A 122 1.698 63.577 33.257 1.00100.85
6
ATOM 910 C MET A 122 3.476 67.369 30.882 1.00 47.40
6
ATOM 911 O MET A 122 2.537 68.082 31.221 1.00 47.59
8
ATOM 912 N ASN A 123 4.525 67.822 30.206 1.00100.32
7
ATOM 913 CA ASN A 123 4.660 69.225 29.861 1.00 99.15
6
ATOM 914 CB ASN A 123 5.979 69.765 30.419 1.00 60.16
6
ATOM 915 CG ASN A 123 7.193 68.984 29.924 1.00 58.49
6
ATOM 916 OD1 ASN A 123 7.088 67.809 29.560 1.00 56.34
8
ATOM 917 ND2 ASN A 123 8.357 69.629 29.941 1.00 57.81
7
ATOM 918 C ASN A 123 4.586 69.556 28.387 1.00 98.67
6
ATOM 919 0 ASN A 123 5.610 69.847 27.769 1.00 99.56
8
ATOM 920 N PRO A 124 3.379 69.523 27.798 1.00 44.27
7
ATOM 921 CD PRO A 124 2.068 69.317 28.425 1.00 98.26
6
ATOM 922 CA PRO A 124 3.228 69.845 26.375 1.00 43.49
6
ATOM 923 CB PRO A 124 1.720 69.776 26.166 1.00 98.08
6
ATOM 924 CG PRO A 124 1.177 70.114 27.514 1.00 99.23
6
ATOM 925 C PRO A 124 3.809 71.236 26.091 1.00 42.28
6
ATOM 926 0 PRO A 124 4.800 71.634 26.713 1.00 41.44
8
ATOM 927 N ASP A 125 3.196 71.987 25.181 1.00 72.10
7
ATOM 928 CA ASP A 125 3.715 73.315 24.842 1.00 71.22
6
ATOM 929 CB ASP A 125 3.290 74.367 25.881 1.00 58.39
6
ATOM 930 CG ASP A 125 2.384 73.801 26.968 1.00 59.12
6
ATOM 931 OD1 ASP A 125 1.510 72.971 26.649 1.00 58.91
8
ATOM 932 OD2 ASP A 125 2.528 74.200 28.145 1.00 59.76
8
ATOM 933 C ASP A 125 5.240 73.204 24.792 1.00 69.73
6
ATOM 934 0 ASP A 125 5.796 72.731 23.806 1.00 70.17
8
ATOM 935 N LEU A 126 5.913 73.620 25.858 1.00 29.24
7
ATOM 936 CA LEU A 126 7.367 73.523 25.929 1.00 29.23
6
ATOM 937 CB LEU A 126 7.729 72.167 26.552 1.00 23.23
6
ATOM 938 CG LEU A 126 9.163 71.658 26.398 1.00 22.23
6
ATOM 939 CD1 LEU A 126 10.174 72.770 26.649 1.00 22.69
' 6
ATOM 940 CD2 LEU A 126 9.382 70.502 27.347 1.00 20.55
6
ATOM 941 C LEU A 126 8.036 73.707 24.557 1.00 29.26
6
ATOM 942 O LEU A 126 8.539 72.755 23.963 1.00 29.35
8
ATOM 943 N HIS A 127 8.045 74.942 24.062 1.00 67.12
7
17 .

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 944 CA HISA 127 8.619 75.246 22.747 1.00 68.12
6
ATOM 945 CB HISA 127 8.936 76.739 22.638 1.00 79.55
6
ATOM 946 CG HISA 127 9.494 77.146 21.306 1.00 81.29
6
ATOM 947 CD2 HISA 127 10.036 76.418 20.299 1.00 81.61
6
ATOM 948 ND1 HISA 127 9.542 78.461 20.895 1.00 82.58
7
ATOM 949 CE1 HISA 127 10.091 78.526 19.696 1.00 82.91
6
ATOM 950 NE2 HISA 127 10.399 77.301 19.311 1.00 82.76
7
ATOM 951 C HISA 127 9.862 74.450 22.375 1.00 67.26
6
ATOM 952 0 HISA 127 10.961 74.800 22.780 1.00 67.73
8
ATOM 953 N ILEA 128 9.698 73.415 21.561 1.00 26.95
7
ATOM 954 CA ILEA 128 10.830 72.595 21.176 1.00 26.01
6
ATOM 955 CB ILEA 128 10.383 71.144 21.000 1.00 19.30
6
ATOM 956 CG2 ILEA 128 11.557 70.246 20.617 1.00 18.95
6
ATOM 957 CG1 ILEA 128 9.788 70.660 22.303 1.00 18.10
6
ATOM 958 CD1 ILEA 128 10.741 70.757 23.436 1.00 18.67
6
ATOM 959 C ILEA 128 11.618 73.032 19.937 1.00 26.70
6
ATOM 960 O ILEA 128 12.840 72.839 19.889 1.00 26.62
8
ATOM 961 N ALAA 129 10.947 73.614 18.939 1.00 61.74
7
ATOM 962 CA ALAA 129 11.644 74.037 17.712 1.00 63.00
6
ATOM 963 CB ALAA 129 12.309 '72.821 17.053 1.00 29.93
6
ATOM 964 C ALAA 129 10.795 74.780 16.673 1.00 62.52
6
ATOM 965 O ALAA 129 10.032 74.176 15.920 1.00 62.23
8
ATOM 966 N THRA 130 10.957 76.095 16.626 1.00 42.47
7
ATOM 967 CA THRA 130 10.222 76.922 15.685 1.00 44.16
6
ATOM 968 CB THRA 130 10.261 78.415 16.114 1.00 37.02
6
ATOM 969 OG1 THRA 130 9.345 78.624 17.193 1.00 36.43
8
ATOM 970 CG2 THRA 130 9.874 79.329 14.972 1.00 38.61
6
ATOM 971 C THRA 130 10.781 76.773 14.271 1.00 44.81
6
ATOM 972 0 THRA 130 11.369 77.711 13.718 1:00 45.51
8
ATOM 973 N LEUA 131 10.597 75.590 13.684 1.00 69.43
7
ATOM 974 CA LEUA 131 11.078 75.343 12.324 1.00 70.26
6
ATOM 975 CB LEUA 131 11.388 73.844 12.126 1..0023.62
6
ATOM 976 CG LEUA 131 10.496 72.762 12.723 1.00 20.66
6
ATOM 977 CD1 LEUA 131 9.163 72.821 12.018 1.00 20.91
6
ATOM 978 CD2 LEUA 131 11.130 71.373 12.573 1.00 18.41
6
ATOM 979 C LEUA 131 10.085 75.865 11.275 1.00 ?1.29
6
ATOM 980 O LEUA 131 8.915 76.101 11.587 1.00 71.02
8
ATOM 981 N GLUA 132 10.552 76.073 10.045 1.00 34.39
7
ATOM 982 CA GLUA 132 9.665 76.587 9.009 1.00 37.30
6
ATOM 983 CB GLUA 132 9.125 77.960 9.414 1.00144.24
6
ATOM 984 CG GLUA 132 10.175 79.051 9.488 1.00146.96
6
ATOM 985 CD GLUA 132 9.600 80.377 9.947 1.00148.66
6
ATOM 986 OE1 GLUA 132 9.088 80.435 11.084 1.00149.93
8
ATOM 987 OE2 GLUA 132 9.656 81.356 9.174 1.00149.02
8
ATOM 988 C GLUA 132 10.300 76.691 7.633 1.00 37.42
6
ATOM 989 O GLUA 132 11.519 76.702 7.504 1.00 36.86
8
ATOM 990 N GLUA 133 9.447 76.785 6.612 1.00 74.32
7
ATOM 991 CA GLUA 133 9.860 76.865 5.211 1.00 76.30
6
ATOM 992 CB GLUA 133 10.583 78.192 4.931 1.00107.76
6
ATOM 993 CG GLUA 133 9.686 79.264 4.291 1.00110.53
6
ATOM 994 CD GLUA 133 10.452 80.510 3.820 1.00112.96
6
ATOM 995 OE1 GLUA 133 11.503 80.360 3.155 1.00114.35
8
ATOM 996 OE2 GLUA 133 9.991 81.642 4.099 1.00113.77
8
ATOM 997 C GLUA 133 10.735 75.674 4.796 1.00 76.32
6
ATOM 998 O GLUA 133 11.134 75.556 3.641 1.00 76.78
8
ATOM 999 N GLYA 134 11.012 74.786 5.748 1.00 49.88
7
18

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1000 CA GLY A 134 11.824 73.610 5.486 1.00 49.15
6
ATOM 1001 C GLY A 134 11.557 72.535 6.528 1.00 48.48
6
ATOM 1002 O GLY A 134 12.406 72.236 7.367 1.00 47.28
8
ATOM 1003 N GLY A 135 10.362 71.956 6.484 1.00100.77
~
7
ATOM 1004 CA GLY A 135 10.015 70.916 7.433 1.00100.88
6
ATOM 1005 C GLY A 135 11.060 69.829 7.445 1.00100.39
6
ATOM 1006 0 GLY A 135 11.122 68..995 6.541 1.00101.39
8
ATOM 1007 N LYS A 136 11.889 69.832 8.477 1.00 53.75
7
ATOM 1008 CA LYS A 136 12.947 68.844 8.579 1.00 52.81
6
ATOM 1009 CB LYS A 136 14.117 69.281 7.689 1.00 74.23
6
ATOM 1010 CG LYS A 136 15.236 68.259 7.540 1.00 75.69
6
ATOM 1011 CD LYS A 136 14.739 66.966 6.914 1.00 75.71
6
ATOM 1012 CE LYS A 136 15.869 65.960 6.800 1.00 76.06
6
ATOM 1013 NZ LYS A 136 15.425 64.618 6.316 1.00 77.03
7
ATOM 1014 C LYS A 136 13.406 68.730 10.028 1.00 50.77
6
ATOM 1015 O LYS A 136 14.026 69.653 10.542 1.00 50.85
8
ATOM 1016 N LEU A 137 13.116 67.611 10.691 1.00 63.98
7
ATOM 1017 CA LEU A 137 13.541 67.485 12.074 1.00 62.44
6
ATOM 1018 CB LEU A 137 12.599 68.281 12.968 1.00 36.13
6
ATOM 1019 CG LEU A 137 13.185 68.463 14.370 1.00 34.38
6
ATOM 1020 CD1 LEU A 137 14.506 69.223 14.245 1.00 34.17
6
ATOM 1021 CD2 LEU A 137 12.211 69.201 15.277 1.00 33.90
6
ATOM 1022 C LEU A 137 13.742 66.105 12.703 1.00 61.48
6
ATOM 1023 O LEU A 137 14.491 65.990 13.667 1.00 62.25
8
ATOM 1024 N TYR A 138 13.111 65.063 12.169 1.00 28.02
7
ATOM 1025 CA TYR A 138 13.216 63.715 12.760 1.00 26.33
6
ATOM 1026 CB TYR A 138 13.480 62.626 11.710 1.00 33.29
6
ATOM 1027 CG~ TYR A 138 13.352 61.254 12.349 1.00 30.22
6
ATOM 1028 CD1 TYR A 138 12.109 60.764 12.741 1.00 27.96
6
ATOM 1029 CE1 TYR A 138 11.996 59.605 13.494 1.00 25.43
6
ATOM 1030 CD2 TYR A 138 14.477 60.528 12.716 1.00 28.72
6
ATOM 1031 CE2 TYR A 138 14.369 59.360 13.476 1.00 26.35
6
ATOM 1032 CZ TYR A 138 13.128 58.911 13.868 1.00 24.91
6
ATOM 1033 OH TYR A 138 13.038 57.813 14.691 1.00 22.73
8
ATOM 1034 C TYR A 138 14.234 63.532 13.878 1.00 25.01
6
ATOM 1035 O TYR A 138 15.438 63.496 13.622 1.00 23.65
8
ATOM 1036 N MET A 139 13.734 63.385 15.106 1.00 33.00
7
ATOM 1037 CA MET A 139 14.595 63.210 16.272 1.00 35.08
6
ATOM 1038 CB MET A 139 14.544 64.450 17.174 1.00 79.45
6
ATOM 1039 CG MET A 139 14.819 65.770 16.493 1.00 83.48
6
ATOM 1040 SD MET A 139 14.970 67.100 17.691 1.00 86.79
16
ATOM 1041 CE MET A 139 13.312 67.231 18.277 1.00 86.81
6
ATOM 1042 C MET A 139 14.271 62.000 17.150 1.00 34.13
6
ATOM 1043 O MET A 139 13.125 61.795 17.525 1.00 34.95
8
ATOM 1044 N GLU A 140 15.286 61.206 17.476 1.00 57.41
7
ATOM 1045 CA GLU A 140 15.118 60.063 18.367 1.00 56.32
6
ATOM 1046 CB GLU A 140 15.884 58.852 17.847 1.00 58.63
6
ATOM 1047 CG GLU A 140 15.386 58.247 16.555 1.00 58.67
6
ATOM 1048 CD GLU A 140 16.270 57.087 16.109 1.00 58.99
6
ATOM 1049 OE1 GLU A 140 16.632 56.255 16.972 1.00 58.20
8
ATOM 1050 OE2 GLU A 140 16.601 56.996 14.907 1.00 58.51
8
ATOM 1051 C GLU A 140 15.786 60.576 19.646 1.00 55.08
6
ATOM 1052 O GLU A 140 16.996 60.810 19.649 1.00 55.57
8
ATOM 1053 N VAL A 141 15.024 60.752 20.725 1.00 13.87
7
ATOM 1054 CA VAL A 141 15.589 61.296 21.968 1.00 13.87
6
ATOM 1055 CB VAL A 141 15.108 62.767 22.184 1.00 30.35
6
19

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1056 CG1 VALA 141 13.773 63.001 21.501 1.00 30.27
ATOM 1057 CG2 VALA 141 14.992 63.081 23.658 1.00 30.89
ATOM 1058 C VALA 141 15.340 60.502 23.232 1.00 13.87
ATOM 1059 0 VALA 141 14.292 60.615 23.837 1.00 13.87
ATOM 1060 N ARGA 142 16.332 59.728 23.644 1.00 15.97
ATOM 1061 CA ARGA 142 16.230 58.885 24.832 1.00 17.66
ATOM 1062 CB ARGA 142 17.620 58.497 25.319 1.00 36.74
ATOM 1063 CG ARGA 142 17.799 57.015 25.461 1.00 35.26
ATOM 1064 CD ARGA 142 18.739 56.701 26.556 1.00 33.63
ATOM 1065 NE ARGA 142 18.991 55.274 26.633 1.00 33.56
ATOM 1066 CZ ARGA 142 19.798 54.715 27.530 1.00 35.05
ATOM 1067 NH1 ARGA 142 20.415 55.482 28.419 1.00 36.63
ATOM 1068 NH2 ARGA 142 20.006 53.398 27.540 1.00 35.26
ATOM 1069 C ARGA 142 15.469 59.522 25.983 1.00 18.25
ATOM 1070 0 ARGA 142 15.072 60.668 25.876 1.00 17.69
ATOM 1071 N VALA 143 15.262 58.765 27.069 1.00 52.69
ATOM 1072 CA VALA 143 14.570 59.241 28.285 1.00 55.71
ATOM 1073 CB VALA 143 13.054 59.520 28.052 1.00156.86
ATOM 1074 CG1 VALA 143 12.845 60.943 27.562 1.00158.32
ATOM 1075 CG2 VALA 143 12.485 58.534 27.060 1.00159.44
ATOM 1076 C VALA 143 14.678 58.266 29.463 1.00 55.82
ATOM 1077 0 VALA 143 13.678 57.742 29.938 1.00 56.81
ATOM 1078 N ASPA 144 15.903 58.048 29.933 1.00 35.56
ATOM 1079 CA ASPA 144 16.206 57.144 31.048 1.00 36.24
ATOM 1080 CB ASPA 144 17.717 56.993 31.235 1.00 43.33
ATOM 1081 CG ASPA 144 18.337 56.016 30.264 1.00 44.54
ATOM 1082 OD1 ASPA 144 17.875 55.965 29.096 1.00 44.64
ATOM 1083 OD2 ASPA 144 19.298 55.312 30.672 1.00 44.72
ATOM 1084 C ASPA 144 15.647 57.566 32.378 1.00 35.85
ATOM 1085 O ASP~A 144 15.210 58.701 32.568 1.00 35.46
ATOM 1086 N ARGA 145 15.705 56.626 33.310 1.00 58.26
ATOM 1087 CA ARGA 145 15.223 56.844 34.659 1.00 61.18
ATOM 1088 CB ARGA 145 14.411 55.625 35.120 1.00 90.29
ATOM 1089 CG ARGA 145 13.329 55.909 36.166 1.00 92.68
ATOM 1090 CD ARGA 145 12.117 54.997 35.952 1.00 93.22
ATOM 1091 NE ARGA 145 10.965 55.394 36.759 1.00 95.42
ATOM 1092 CZ ARGA 145 9.702 55.118 36.438 1.00 96.41
ATOM 1093 NH1 ARGA 145 9.433 54.447 35.325 1.00 95.91
ATOM 1094 NH2 ARGA 145 8.704 55.509 37.225 1.00 97.11
ATOM 1095 C ARGA 145 16.487 56.995 35.484 1.00 61.07
ATOM 1096 0 ARGA 145 17.301 56.077 35.540 1.00 61.52
ATOM 1097 N GLYA 146 16.665 58.158 36.103 1.00 50.34
ATOM 1098 CA GLYA 146 17.858 58.386 36.897 1.00 49.87
ATOM 1099 C GLYA 146 17.552 58.932 38.272 1.00 49.74
ATOM 1100 0 GLYA 146 16.400 58.953 38.706 1.00 49.96
ATOM 1101 N VALA 147 18.598 59.380 38.956 1.00 48.38
ATOM 1102 CA VALA 147 18.475 59.928 40.300 1.00 47.31
ATOM 1103 CB VALA 147 18.679 58.834 41.358 1.00 24.62
ATOM 1104 CG1 VALA 147 18.986 59.458 42.698 1.00 25.16
ATOM 1105 CG2 VALA 147 17.441 57.975 41.450 1.00 23.97
ATOM 1106 C VALA 147 19.507 61.013 40.537 1.00 46.92
ATOM 1107 O VALA 147 20.706 60.797 40.353 1.00 47.27
ATOM 1108 N GLYA 148 19.038 62.179 40.951 1.00 41.92
ATOM 1109 CA GLYA 148 19.952 63.271 41.210 1.00 42.13
ATOM 1110 C GLYA 148 20.387 63.969 39.941 1.00 41.88
ATOM 1111 O GLYA 148 19.615 64.060 38.989 1.00 41.60

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1112 N TYR A 149 21.633 64.443 39.931 1.00 56.71 7
ATOM 1113 CA TYR A 149 22.211 65.167 38.798 1.00 56.25 6
ATOM 1114 CB TYR A 149 22.611 66.564 39.267 1.00 33.62 6
ATOM 1115 CG TYR A 149 23.243 67.432 38.220 1.00 29.72 6
ATOM 1116 CD1 TYR A 149 22.743 67.479 36.925 1.00 27.89 6
ATOM 1117 CE1 TYR A 149 23.279 68.353 35.971 1.00 25.22 6
ATOM 1118 CD2 TYR A 149 24.300 68.274 38.542 1.00 28.71 6
ATOM 1119 CE2 TYR A 149 24.842 69.153 37.600 1.00 26.48 6
ATOM 1120 CZ TYR A 149 24.323 69.188 36.318 1.00 24.38 6
ATOM 1121 OH TYR A 149 24.840 70.082 35.409 1.00 20.70 8
ATOM 1122 C TYR A 149 23.429 64.449 38.216 1:00 57.82 6
ATOM 1123 O TYR A 149 24.454 64.334 38.884 1.00 58.18 8
ATOM 1124 N VAL A 150 23.318 63.971 36.976 1.00 25.17 7
ATOM 1125 CA VAL A 150 24.426 63.258 36.318 1.00 27.90 6
ATOM 1126 CB VAL A 150 24.146 61.733 36.207 1.00 43.10 6
ATOM 1127 CG1 VAL A 150 25.343 61.022 35.593 1.00 43.85 6
ATOM 1128 CG2 VAL A 150 23.836 61.150 37.578 1.00 43.89 6
ATOM 1129 C VAL A 150 24.683 63.794 34.915 1.00 30.48 6
ATOM 1130 O VAL A 150 23.971 63.450 33.986 1.00 31.06 8
ATOM 1131 N PRO A 151 25.715 64.642 34.754 1.00 54.43 7
ATOM 1132 CD PRO A 151 26.346 65.312 35.904 1.00128.16 6
ATOM 1133 CA PRO A 151 26.146 65.283 33.507 1.00 57.01 6
ATOM 1134 CB PRO A 151 27.167 66.306 33.991 1.00129.50 6
ATOM 1135 CG PRO A 151 26.658 66.665 35.336 1.00129.68 6
ATOM 1136 C PRO A 151 26.716 64.407 32.387 1.00 59.77 6
ATOM 1137 O PRO A 151 26.354 63.243 32.226 1.00 60.40 8
ATOM 1138 N ALA A 152 27.615 65.001 31.611 1.00 72.84 7
ATOM 1139 CA ALA A 152 28.259 64.345 30.478 1.00 76.09 6
ATOM 1140 CB ALA A 152 29.206 65.329 29.774 1.00 20.79 6
ATOM 1141 C ALA A 152 29.007' 63.063 30.811 1.00 78.69 6
ATOM 1142 O ALA A 152 29.763 62.562 29.983 1.00 79.51 8
ATOM 1143 N GLU A 153 28.823 62.534 32.013 1.00107.48 7
ATOM 1144 CA GLU A 153 29.483 61.283 32.357 1.00110.39 6
ATOM 1145 CB GLU A 153 29.558 61.104 33.869 1.00169.79 6
ATOM 1146 CG GLU A 153 29.938 59.697 34.286 1.00173.04 6
ATOM 1147 CD GLU A 153 30.069 59.550 35.782 1.00175.00 6
ATOM 1148 OE1 GLU A 153 29.155 60.001 36.506 1.00175.25 8
ATOM 1149 OE2 GLU A 153 31.082 58.977 36.237 1.00176.25 8
ATOM 1150 C GLU A 153 28.674 60.135 31.741 1.00110.83 6
ATOM 1151 O GLU A 153 29.210 59.299 31.013 1.00110.62 8
ATOM 1152 N ARG A 154 27.378 60.107 32.037 1.00163.36 7
ATOM 1153 CA ARG A 154 26.486 59.080 31.509 1.00164.22 6
ATOM 1154 CB ARG A 154 25.102 59.221 32.158 1.00114.78 6
ATOM 1155 CG ARG A 154 24.054 58.215 31.684 1.00115.49 6
ATOM 1156 CD ARG A 154 22.905 58.046 32.703 1.00115.63 6
ATOM 1157 NE ARG A 154 23.354 57.411 33.947 1.00115.68 7
ATOM 1158 CZ ARG A 154 22.563 57.085 34.968 1.00115.42 6
ATOM 1159 NH1 ARG A 154 21.261 57.329 34.912 1.00114.73 7
ATOM 1160 NH2 ARG A 154 23.080 56.515 36.051 1.00115.47 7
ATOM 1161 C ARG A 154 26.387 59.257 29.997 1.00164.11 6
ATOM 1162 O ARG A 154 25.448 59.885 29.504 1.00164.81 8
ATOM 1163 N HIS A 155 27.353' 58.698 29.267 1.00 75.45 7
ATOM 1164 CA HIS A 155 27.383 58.827 27.810 1.00 74.77 6
ATOM 1165 CB HIS A 155 28.447 57.910 27.201 1.00177.89 6
ATOM 1166 CG HIS A 155 28.748 58.216 25.767 1.00178.95 6
ATOM 1167 CD2 HIS A 155 28.569 57.486 24.641 1.00179.71 6
21

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1168 ND1 HIS A 155 29.281 59.421 25.361 1.00179.47 7
ATOM 1169 CE1 HIS A 155 29.416 59.420 24.047 1.00179.70 6
ATOM 1170 NE2 HIS A 155 28.990 58.258 23.586 1.00179.98 7
ATOM 1171 C HIS A 155 26.034 58.559 27.148 1.00 73.51 6
ATOM 1172 0 HIS A 155 25.253 57.708 27.594 1.00 72.88 8
ATOM 1173 N GLY A 156 25.775 59.297 26.073 1.00 63.99 7
ATOM 1174 CA GLY A 156 24.516 59.171 25.361 1.00 62.57 6
ATOM 1175 C GLY A 156 24.550 58.229 24.184 1.00 61.40 6
ATOM 1176 0 GLY A 156 23.700 58.300 23.302 1.00 60.43 8
ATOM 1177 N ILE A 157 25.542 57.349 24.182 1.00 73.01 7
ATOM 1178 CA ILE A 157 25.707 56.357 23.135 1.00 72.98.6
ATOM 1179 CB ILE A 157 26.239 55.025 23.747 1.00101.08 6
ATOM 1180 CG2 ILE A 157 25.403 54.632 24.964 1.00101.52 6
ATOM 1181 CG1 ILE A 157 26.255 53.924 22.688 1.00101.27 6
ATOM 1182 CD1 ILE A 157 26.917 52.644 23.140 1.00102.19 6
ATOM 1183 C ILE A 157 24.409 56.117 22.353 1.00 72.00 6
ATOM 1184 0 ILE A 157 24.148 56.808 21.376 1.00 71.90 8
ATOM 1185 N LYS A 158 23.588 55.166 22.795 1.00110.17 7
ATOM 1186 CA LYS A 158 22.333 54.851 22.113 1.00108.68 6
ATOM 1187 CB LYS A 158 21.270 55.916 22.390 1.00 97.31 6
ATOM 1188 CG LYS A 158 20.297 55.551 23.491 1.00 97.28 6
ATOM 1189 CD LYS A 158 19.567 54.257 23.177 1.00 96.09 6
ATOM 1190 CE LYS A 158 18.590 53.895 24.290 1.00 94.87 6
ATOM 1191 NZ LYS A 158 18.012 52.522 24.162 1.00 93.51 7
ATOM 1192 C LYS A 158 22.520 54.727 20.609 1.00107.55 6
ATOM 1193 0 LYS A 158 22.982 53.697 20.115 1.00108.08 8
ATOM 1194 N ASP A 159 22.164 55.790 19.889 1.00 59.19 7
ATOM 1195 CA ASP A 159 22.263 55.839 18.426 1.00 57.80 6
ATOM 1196 CB ASP A 159 21.589 57.125 17.907 1.00 86.33 6
ATOM 1197 CG ASP A 159 21.011 56.968 16.505 1.00 85.93 6
ATOM 1198 OD1 ASP A 159 21.796 56.858 15.544 1.00 86.75 8
ATOM 1199 OD2 ASP A 159 19.768 56.950 16.363 1.00 84.74 8
ATOM 1200 C ASP A 159 23.723 55.786 17.983 1.00 56.46 6
ATOM 1201 0 ASP A 159 24.054 56.164 16.866 1.00 55.59 8
ATOM 1202 N ARG A 160 24.582 55.311 18.881 1.00 87.56 7
ATOM 1203 CA ARG A 160 26.014 55.182 18.631 1.00 88.42 6
ATOM 1204 CB ARG A 160 26.402 53.702 18.522 1.00102.75 6
ATOM 1205 CG ARG A 160 26.715 53.008 19.844 1.00102.79 6
ATOM 1206 CD ARG A 160 27.249 51.598 19.603 1.00102.12 6
ATOM 1207 NE ARG A 160 27.592 50.890 20.833 1.00100.58 7
ATOM 1208 CZ ARG A 160 28.112 49.668 20.860 1.00100.56 6
ATOM 1209 NH1 ARG A 160 28.347 49.025 19.725 1.00100.25 7
ATOM 1210 NH2 ARG A 160 28.400 49.085 22.014 1.00100.27 7
ATOM 1211 C ARG A 160 26.528 55.907 17.393 1.00 87.57 6
ATOM 1212 0 ARG A 160 27.015 55.271 16.456 1.00 88.01 8
ATOM 1213 N ILE A 161 26.424 57.230 17.374 1.00106.49 7
ATOM 1214 CA ILE A 161 26.924 57.961 16.222 1.00104.57 6
ATOM 1215 CB ILE A 161 26.176 57.570 14.931 1.00 48.58 6
ATOM 1216 CG2 ILE A 161 24.709 57.935 15.052 1.00 47.75 6
ATOM 1217 CG1 ILE A 161 26.816 58.289 13.731 1.00 48.28 6
ATOM 1218 CD1 ILE A 161 26.382 57.7.7712.355 1.00 48.64 6
ATOM 1219 C ILE A 161 26.851 59.464 16.347 1.00103.31 6
ATOM 1220 O ILE A 161 27.860 60.161 16.218 1.00103.93 8
ATOM 1221 N ASN A 162 25.651 59.964 16.597 1.00 73.05 7
ATOM 1222 CA ASN A 162 25.453 61.396 16.686 1.00 70.40 6
ATOM 1223 CB ASN A 162 24.800 61.873 15.392 1.00 45.57 6
22

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1224 CG ASN A 162 25.310 61.112 14.168 1.00 45.25
6
ATOM 1225 OD1 ASN A 162 26.494 61.185 13.807 1.00 44.13
8
ATOM 1226 ND2 ASN A 162 24.412 60.371 13.528 1.00 44.20
7
ATOM 1227 C ASN A 162 24.569 61.701 17.876 1.00 68.28
6
ATOM 1228 O ASN A 162 23.898 62.728 17.924 1.00 68.79
8
ATOM 1229 N ALA A 163 24.586 60.787 18.837 1.00 49.85
7
ATOM 1230 CA ALA A 163 23.784 60.911 20.039 1.00 46.49
6
ATOM 1231 CB ALA A 163 24.181 59.824 21.022 1.00113.93
6
ATOM 1232 C ALA A 163 23.876 62.279 20.709 1.00 43.84
6
ATOM 1233 O ALA A 163 22.933 62.712 21.366 1.00 42.33
8
ATOM 1234 N ILE A 164 25.003 62.960 20.550 1.00 44.78
7
ATOM 1235 CA ILE A 164 25.183 64.267 21.171 1.00 41.25
6
ATOM 1236 CB ILE A 164 24.538 65.390 20.309 1.00 14.84
6
ATOM 1237 CG2 ILE A 164 24.860 65.165 18.847 1.00 13.87
6
ATOM 1238 CG1 ILE A 164 23.023 65.399 20.465 1.00 13.87
6
ATOM 1239 CD1 ILE A 164 22.358 66.472 19.666 1.00 13.87
6
ATOM 1240 C ILE A 164 24.560 64.289 22.570 1.00 40.59
6
ATOM 1241 O ILE A 164 23.845 65.219 22.913 1.00 41.32
8
ATOM 1242 N PRO A 165 24.867 63.282 23.407 1.00 38.68
7
ATOM 1243- CD PRO A 165 26.175 62.626 23.304 1.00 32.21
6
ATOM 1244 CA PRO A 165 24.358 63.127 24.778 1.00 37.84
6
ATOM 1245 CB PRO A 165 25.469 62.357 25.478 1.00 31.90
6
ATOM 1246 CG PRO A 165 26.670 62.746 24.710 1.00 31.31
6
ATOM 1247 C PRO A 165 24.057 64.433 25.473 1.00 38.07
6
ATOM 1248 O PRO A 165 24.861 65.366 25.398 1.00 38.33
8
ATOM 1249 N VAL A 166 22.908 64.488 26.152 1.00 28.76
7
ATOM 1250 CA VAL A 166 22.462 65.687 26.869 1.00 28.17
6
ATOM 1251 CB VAL A 166 21.018 66.014 26.538 1.00 37.29
6
ATOM 1252 CG1 VAL A 166 20.639 67.328 27.174 1.00 37.95
6
ATOM 1253 CG2 VAL A 166 20.830 66.045 25.056 1.00 37.42
6
ATOM 1254 C VAL A 166 22.532 65.548 28.384 1.00 28.88
6
ATOM 1255 0 VAL A 166 22.344 64.452 28.927 1.00 29.93
8
ATOM 1256 N ASP A 167 22.780 66.659 29.070 1.00 27.15
7
ATOM 1257 CA ASP A 167 22.847 66.627 30.525 1.00 28.82
6
ATOM 1258 CB ASP A 167 23.131 68.019 31.079 1.00 55.43
6
ATOM 1259 CG ASP A 167 24.512 68.503 30.750 1.00 56.58
6
ATOM 1260 OD1 ASP A 167 25.452 67.682 30.781 1.00 57.79
8
ATOM 1261 OD2 ASP A 167 24.654 69.709 30.478 1.00 55.60
8
ATOM 1262 C ASP A 167 21.540 66.119 31.133 1.00 28.92
6
ATOM 1263 O ASP A 167 20.459 66.350 30.589 1.00 29.75
8
ATOM 1264 N ALA A 168 21.632 65.448 32.271 1.00 33.33
7
ATOM 1265 CA ALA A 168 20.445 64.928 32.920 1.00 35.59
6
ATOM 1266 CB ALA A 168 20.649 63.459 33.243 1.00 46.87
6
ATOM 1267 C ALA A 168 20.098 65.700 34.193 1.00 37.01
6
ATOM 1268 O ALA A 168 20.974 66.214 34.871 1.00 37.01
8
ATOM 1269 N ILE A 169 18.810 65.789 34.500 1.00 41.48
7
ATOM 1270 CA ILE A 169 18.325 66.460 35.702 1.00 43.82
6
ATOM 1271 CB ILE A 169 17.784 67.873 35.411 1.00 66.79
6
ATOM 1272 CG2 ILE A 169 17.951 68.745 36.637 1.00 67.21
6
ATOM 1273 CG1 ILE A 169 18.515 68.500 34.224 1.00 68.86
6
ATOM 1274 CD1 ILE A 169 19.952 68.843 34.495 1.00 71.35
6
ATOM 1275 C ILE A 169 17.141 65.592 36.096 1.00 44.59
6
ATOM 1276 O ILE A 169 16.015 65.859 35.670 1.00 45.12
8
ATOM 1277 N PHE A 170 17.395 64.559 36.895 1.00 61.53
7
ATOM 1278 CA PHE A 170 16.356 63.613 37.308 1.00 63.83
6
ATOM 1279 CB PHE A 170 17.021 62.303 37.715 1.00 95.45
6
23

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1280 CG PHE A 170 17.810 61.673 36.614 1.00 98.05
ATOM 1281 CD1 PHE A 170 17.184 61.259 35.450 1.00 97.88
ATOM 1282 CD2 PHE A 170 19.182 61.512 36.728 1.00 99.39
ATOM 1283 CE1 PHE A 170 17.914 60.693 34.418 1.00 97.26
ATOM 1284 CE2 PHE A 170 19.921 60.946 35.699 1.00 98.86
ATOM 1285 CZ PHE A 170 19.288 60.539 34.544 1.00 97.30
ATOM 1286 C PHE A 170 15.333 64.023 38.378 1.00 64.23
ATOM 1287 0 PHE A 170 14.134 63.787 38.207 1.00 64.72
ATOM 1288 N SER A 171 15.785 64.620 39.476 1.00 39.73
ATOM 1289 CA SER A 171 14.868 65.024 40.540 1.00 38.09
ATOM 1290 CB SER A 171 15.535 66.065 41.444 1.00 78.49
ATOM 1291 OG SER A 171 14.693 66.419 42.525 1.00 80.28
ATOM 1292 C SER A 171 13.556 65.592 39.987 1.00 35.93
ATOM 1293 O SER A 171 13.555 66.540 39.201 1.00 36.51
ATOM 1294 N PRO A 172 12.419 64.991 40.369 1.00 48.33
ATOM 1295 CD PRO A 172 12.324 63.681 41.039 1.00 44.87
ATOM 1296 CA PRO A 172 11.098 65.448 39.916 1.00 47.93
ATOM 1297 CB PRO A 172 10.238 64.206 40.068 1.00 43.94
ATOM 1298 CG PRO A 172 10.838 63.550 41.291 1.00 44.29
ATOM 1299 C PRO A 172 10.631 66.582 40.814 1.00 46.83
ATOM 1300 0 PRO A 172 9.736 67.335 40.472 1.00 47.00
ATOM 1301 N VAL A 173 11.252 66.679 41.982 1.00 41.35
ATOM 1302 CA VAL A 173 10.950 67.721 42.952 1.00 40.25
ATOM 1303 CB VAL A 173 11.693 67.420 44.270 1.00 29.43
ATOM 1304 CG1 VAL A 173 11.439 68.512 45.283 1.00 30.41
ATOM 1305 CG2 VAL A 173 11.245 66.063 44.815 1.00 27.70
ATOM 1306 C VAL A 173 11.445 69.031 42.342 1.00 39.45
ATOM 1307 O VAL A 173 12.603 69.127 41.955 1.00 39.32
ATOM 1308 N ARG A 174 10.589 70.038 42.237 1.00 59.70
ATOM 1309 CA ARG A 174 11.034 71.288 41.625 1.00 62.26
ATOM 1310 CB ARG A 174 10.105 71.677 40.469 1.00140.03
ATOM 1311 CG ARG A 174 10.243 70.821 39.208 1.00144.79
ATOM 1312 CD ARG A 174 11.541 71.100 38.433 1.00149.38
ATOM 1313 NE ARG A 174 11.635 70.294 37.212 1.00151.23
'
ATOM 1314 CZ ARG A 174 12.669 70.302 36.373 1.00151.78
ATOM 1315 NH1 ARG A 174 13.719 71.079 36.609 1.00152.98
ATOM 1316 NH2 ARG A 174 12.658 69.523 35.298 1.00151.43
ATOM 1317 C ARG A 174 11.190 72.490 42.555 1.00 61.59
ATOM 1318 0 ARG A 174 11.310 73.619 42.079 1.00 60.56
ATOM 1319 N ARG A 175 11.207 72.252 43.865 1.00 50.49
ATOM 1320 CA ARG A 175 11.345 73.323 44.853 1.00 48.86
ATOM 1321 CB ARG A 175 10.736 74.609 44.312 1.00 54.88
ATOM 1322 CG ARG A 175 10.585 75.698 45.317 1.00 54.77
ATOM 1323 CD ARG A 175 10.180 76.974 44.623 1.00 53.43
ATOM 1324 NE ARG A 175 9.537 77.896 45.545 1.00 53.72
ATOM 1325 CZ ARG A 175 9.203 79.137 45.232 1.00 53.04
ATOM 1326 NH1 ARG A 175 9.464 79.596 44.017 1.00 53.52
ATOM 1327 NH2 ARG A 175 8.591 79.908 46.122 1.00 52.20
ATOM 1328 C ARG A 175 10.610 72.895 46.109 1.00 48.09
ATOM 1329 O ARG A 175 9.603 72.218 46.010 1.00 49.40
ATOM 1330 N VAL A 176 11.104 73.282 47.282 1.00 34.91
ATOM 1331 CA VAL A 176 10.473 72.902 48.552 1.00 35.21
ATOM 1332 CB VAL A 176 10.989 71.547 49.077 1.00 13.87
ATOM 1333 CG1 VAL A 176 10.037 71.013 50.122 1.00 13.87
ATOM 1334 CG2 VAL A 176 11.208 70.567 47.947 1.00 13.87
ATOM 1335 C VAL A 176 10.769 73.881 49.683 1.00 36.88
24

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1336 O VALA 176 11.909 74.302 49.860 1.00 37.11 8
ATOM 1337 N ALAA 177 9.752 74,.213 50.468 1.00 45.24 7
ATOM 1338 CA ALAA 177 9.925 75.106 51.619 1.00 47.00 6
ATOM 1339 CB ALAA 177 9.479 76.520 51.272 1.00 36.43 6
ATOM 1340 C ALAA 177 9.082 74.55'3 52.769 1.00 48.64 6
ATOM 1341 0 ALAA 177 8.533 73.459 52.661 1.00 48.85 8
ATOM 1342 N PHEA 178 8.983 75.282 53.873 1.00 98.12 7
ATOM 1343 CA PHEA 178 8.156 74.790 54.961 1.00100.59 6
ATOM 1344 CB PHEA 178 8.641 73.404 55.393 1.00 40.54 6
ATOM 1345 CG PHEA 178 9.908 73.401 56.184 1.00 42.13 6
ATOM 1346 CD1 PHEA 178 9.878 73.537 57.567 1.00 42.94 6
ATOM 1347 CD2 PHEA 178 11.126 73.173 55.563 1.00 43.85 6
ATOM 1348 CE1 PHEA 178 11.046 73.437 58.325 1.00 46.29 6
ATOM 1349 CE2 PHEA 178 12.304 73.071 56.311 1.00 46.44 6
ATOM 1350 CZ PHEA 178 12.263 73.200 57.696 1.00 47.46 6
ATOM 1351 C PHEA 178 8.020 75.719 56.154 1.00103.23 6
ATOM 1352 O PHEA 178 8.988 75.976 56.866 1.00105.47 8
ATOM 1353 N GLNA 179 6.803 76.224 56.359 1.00 36.08 7
ATOM 1354 CA GLNA 179 6.512 77.136 57.459 1.00 37.00 6
ATOM 1355 CB GLNA 179 5.137 77.766 57.254 1.00 92.61 6
ATOM 1356 CG GLNA 179 4.987 78.522 55.951 1.00 96.35 6
ATOM 1357 CD GLNA 179 5.787 79.805 55.924 1.00 99.08 6
ATOM 1358 OE1 GLNA 179 7.015 79.782 55.942 1.00101.45 8
ATOM 1359 NE2 GLNA 179 5.090 80.938 55.889 1.00 99.95 7
ATOM 1360 C GLNA 179 6.536 76.385 58.784 1.00 37.83 6
ATOM 1361 O GLNA 179 6.288 75.183 58.822 1.00 38.53 8
ATOM 1362 N VALA 180 6.852 77.085 59.869 1.00 74.13 7
ATOM 1363 CA VALA 180 6.873 76.453 61.180 1.00 76.69 6
ATOM 1364 CB VALA 180 8.290 76.399 61.781 1.00 62.85 6
ATOM 1365 CG1 VALA 180 8.292 75.548 63.055 1.00 62.38 6
ATOM 1366 CG2 VALA 180 9.256 75.814 60.773 1.00 63.79 6
ATOM 1367 C VALA 180 5.977 77.248 62.110 1.00 78.88 6
ATOM 1368 O VALA 180 6.244 77.347 63.307 1.00 79.49 8
ATOM 1369 N GLUA 181 4.912 77.809 61.539 1.00 79.29 7
ATOM 1370 CA GLUA 181 3.934 78.610 62.270 1.00 '81.326
ATOM 1371 CB GLUA 181 2.627 78.668 61.501 1.00 65.76 6
ATOM 1372 CG GLUA 181 2.766 79.155 60.093 1.00 67.56 6
ATOM 1373 CD GLUA 181 1.498 78.947 59.293 1.00 68.61 6
ATOM 1374 OE1 GLUA 181 0.434 79.440 59.728 1.00 69.63 8
ATOM 1375 OE2 GLUA 181 1.565 78.289 58.231 1.00 68.52 8
ATOM 1376 C GLUA 181 3.627 78.068 63.652 1.00 83.63 6
ATOM 1377 O GLUA 181 3.874 76.899 63.955 1.00 84.41 8
ATOM 1378 N ASPA 182 3.071 78.937 64.483 1.00 72.84 7
ATOM 1379 CA ASPA 182 2.684 78.576 65.833 1.00 75.42 6
ATOM 1380 CB ASPA 182 3.601 79.262 66.848 1.00117.68 6
ATOM 1381 CG ASPA 182 3.544 80.775 66.762 1.00118.15 6
ATOM 1382 OD1 ASPA 182 3.541 81.309 65.631 1.00117.09 8
ATOM 1383 OD2 ASPA 182 3.514 81.427 67.828 1.00117.92 8
ATOM 1384 C ASPA 182 1.252 79.068 65.974 1.00 77.55 6
ATOM 1385 O ASPA 182 1.010 80.273 66.022 1.00 78.76 8
ATOM 1386 N THRA 183 0.303 78.136 65.998 1.00102.76 7
ATOM 1387 CA THRA 183 -1.110 78.488 66.117 1.00104.69 6
ATOM 1388 CB THRA 183 -1.682 79.018 64.790 1.00 68.16 6
ATOM 1389 OG1 THRA 183 -3.077 79.308 64.966 1.00 69.31 8
ATOM 1390 CG2 THRA 183 -1.505 77.982 63.670 1.00 68.00 6
ATOM 1391 C THRA 183 -1.938 77.285 66.519 1.00106.50 6
2S

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1392 O THR A 183 -2.896 76.922 _65.8351.00106.41
8
ATOM 1393 N ARG A 184 -1.575 76.674 67.638 1.00139.74
7
ATOM 1394 CA ARG A 184 -2.292 75.505 68.101 1.00141.90
6
ATOM 1395 CB ARG A 184 -1.407 74.268 67.905 1.00 97.52
6
ATOM 1396 CG ARG A 184 -0.950 74.092 66:450 1.00 98.60
6
ATOM 1397 CD ARG A 184 -2.150 74.213 65.501 1.00 99.55
6
ATOM 1398 NE ARG A 184 -1.814 74.104 64.082 1.00100.02
7
ATOM 1399 CZ ARG A 184 -2.697 74.265 63.100 1.00 99.96
6
ATOM 1400 NH1 ARG A 184 -3.961 74.545 63.382 1.00 99.41
7
ATOM 1401 NH2 ARG A 184 -2.326 74.127 61.837 1.00100.11
7
ATOM 1402 C ARG A 184 -2.717 75.663 69.554 1.00142.96
6
ATOM 1403 O ARG A 184 -2.285 76.593 70.232 1.00143.20
8
ATOM 1404 N ALA A 185 -3.578 74.769 70.026 1.00187.75
7
ATOM 1405 CA ALA A 185 -4.043 74.836 71.405 1.00188.97
6
ATOM 1406 CB ALA A 185 -5.543 74.605 71.462 1.00138.79
6
ATOM 1407 C ALA A 185 -3.316 73.806 72.260 1.00189.46
6
ATOM 1408 O ALA A 185 -3.080 74.034 73.448 1.00190.37
8
ATOM 1409 N GLY A 186 -2.968 72.680 71.639 1.00113.15
7
ATOM 1410 CA GLY A 186 -2.262 71.609 72.321 1.00111.99
6
ATOM 1411 C GLY A 18G -2.071 71.790 73.815 1.00112.66
6
ATOM 1412 O GLY A 186 -2.942 71.421 74.601 1.00112.80
8
ATOM 1413 N GLN A 187 -0.934 72.360 74.209 1.00115.28
7
ATOM 1414 CA GLN A 187 -0.628 72.584 75.621 1.00115.21
6
ATOM 1415 CB GLN A 187 -0.019 71.318 76.236 1.00172.74
6
ATOM 1416 CG GLN A 187 -0.943 70.103 76.187 1.00174.52
6
ATOM 1417 CD GLN A 187 -0.394 68.908 76.942 1.00175.30
6
ATOM 1418 OE1 GLN A 187 0.693 68.415 76.643 1.00176.13
8
ATOM 1419 NE2 GLN A 187 -1.149 68.431 77.924 1.00175.29
7
ATOM 1420 C GLN A 187 0.338 73.752 75.790 1.00115.15
6
ATOM 1421 O GLN A 187 1.550 73.580 75.696 1.00115.42
8
ATOM 1422 N ARG A 188 -0.207 74.936 76.049 1.00172.13
7
ATOM 1423 CA ARG A 188 0.599 76.141 76.227 1.00170.84
6
ATOM 1424 CB ARG A 188 1.701 75.908 77.268 1.00192.51
6
ATOM 1425 CG ARG A 188 2.709 77.045 77.374 1.00194.75
6
ATOM 1426 CD ARG A 188 3.794 76.741 78.391 1.00197.08
6
ATOM 1427 NE ARG A 188 4.826 77.771 78.390 1.00200.84
7
ATOM 1428 CZ ARG A 188 5.871 77.781 79.208 1.00202.51
6
ATOM 1429 NH1 ARG A 188 6.025 76.811 80.100 1.00204.00
7
ATOM 1430 NH2 ARG A 188 6.765 78.759 79.134 1.00202.86
7
ATOM 1431 C ARG A 188 1.230 76.544 74.903 1.00169.28
6
ATOM 1432 O ARG A 188 2.128 75.865 74.409 1.00168.87
8
ATOM 1433 N THR A 189 0.750 77.649 74.338 1.00187.44
7
ATOM 1434 CA THR A 189 1.259 78.160 73.069 1.00184.73
6
ATOM 1435 CB THR A 189 0.939 79.662 72.903 1.00190.85
6
ATOM 1436 OG1 THR A 189 -0.479 79.859 72.975 1.00191.29
8
ATOM 1437 CG2 THR A 189 1.445 80.172 71.559 1.00190.83
6
ATOM 1438 ~C THR A 189 2.768 77.970 73.008 1.00183.57
6
ATOM 1439 O THR A 189 3.528 78.815 73.482 1.00183.68
8
ATOM 1440 N ASP A 190 3.191 76.852 72.424 1.00 96.77
7
ATOM 1441 CA ASP A 190 4.606 76.525 72.301 1.00 93.47
6
ATOM 1442 CB ASP A 190 5.046 75.638 73.464 1.00145.73
6
ATOM 1443 CG ASP A 190 5.658 76.419 74.598 1.00147.45
6
ATOM 1444 OD1 ASP A 190 6.643 77.144 74.345 1.00148.52
8
ATOM 1445 OD2 ASP A 190 5.163 76.301 75.741 1.00147.80
8
ATOM 1446 C ASP A 190 4.863 75.785 71.003 1.00 92.04
6
ATOM 1447 O ASP A 190 5.592 76.265 70.139 1.00 92.19
8
26

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1448 N LEUA 191 4.253 74.608 70.902 1.00 81.88
7
ATOM 1449 CA LEUA 191 4.351 73.704 69.753 1.00 78.93
6
ATOM 1450 CB LEUA 191 3.047 72.923 69.620 1.00 86.98
6
ATOM 1451 CG LEUA 191 2.224 72.796 70.898 1.00 87.57
6
ATOM 1452 CD1 LEUA 191 0.873 72.186 70.565 1.00 88.40
6
ATOM 1453 CD2 LEUA 191 2.981 71.956 71.911 1.00 88.35
6
ATOM 1454 C LEUA 191 4.661 74.334 68.391 1.00 76.05
6
ATOM 1455 0 LEUA 191 4.416 75.519 68.158 1.00 75.94
8
ATOM 1456 N ASPA 192 5.198 73.520 67.487 1.00 94.98
7
ATOM 1457 CA ASPA 192 5.495 73.991 66.142 1.00 90.90
6
ATOM 1458 CB ASPA 192 6.811 73.386 65.580 1.00 64.72
6
ATOM 1459 CG ASPA 192 7.920 73.233 66.627 1.00 64.42
~ 6
ATOM 1460 OD1 ASPA 192 8.246 74.208 67.333 1.00 65.46
8
ATOM 1461 OD2 ASPA 192 8.486 72.123 66.725 1.00 62.37
8
ATOM 1462 C ASPA 192 4.328 73.486 65.289 1.00 88.07
6
ATOM 1463 0 ASPA 192 3.627 72.550 65.676 1.00 87.89
8
ATOM 1464 N LYSA 193 4.108 74.121 64.147 1.00 18.48
7
ATOM 1465 CA LYSA 193 3.075 73.685 63.230 1.00 13.87
6
ATOM 1466 CB LYSA 193 2.039 74.782 62.995 1.00 68.35
6
ATOM 1467 CG LYSA 193 1.084 74.454 61.853 1.00 70.13
6
ATOM 1468 CD LYSA 193 0.278 75.654 61.402 1.00 71.76
6
ATOM 1469 CE LYSA 193 -0.453 75.341 60.102 1.00 72.69
6
ATOM 1470 NZ LYSA 193 -1.416 76.411 59.712 1.00 74.05
7
ATOM 1471 C LYSA 193 3.772 73.356 61.905 1.00 13.87
6
ATOM 1472 0 LYSA 193 3.733 74.134 60.954 1.00 13.87
8
ATOM 1473 N LEUA 194 4.411 72.194 61.837 1.00 49.50
7
ATOM 1474 CA LEUA 194 5.113 71.801 60.619 1.00 47.28
6
ATOM 1475 CB LEUA 194 5.700 70.408 60.779 1.00 40.96
6
ATOM 1476 CG LEUA 194 7.002 70.187 60.018 1.00 40.77
6
ATOM 1477 CD1 LEUA 194 7.432 68.770 60.271 1.00 42.47
6
ATOM 1478 CD2 LEUA 194 6.838 70.435 58.534 1.00 40.79
6
ATOM 1479 C LEUA 194 4.164 71.819 59.436 1.00 45.66
6
ATOM 1480 0 LEUA 194 2.980 71.566 59.582 1.00 46.31
8
ATOM 1481 N THRA 195 4.673 72.115 58.255 1.00 23.66.
7
ATOM 1482 CA THRA 195 3.795 72.156 57.112 1.00 23.58
6
ATOM 1483 CB THRA 195 2.764 73.333 57.272 1.00 47.51
6
ATOM 1484 OG1 THRA 195 2.457 73.916 55.999 1.00 49.59
8
ATOM 1485 CG2 THRA 195 3.307 74.398 58.183 1.00 49.19
6
ATOM 1486 C THRA 195 4.558 72.275 55.797 1.00 21.65
6
ATOM 1487 0 THRA 195 4.413 73.270 55.077 1.00 20.96
8
ATOM 1488 N LEUA 196 5.359 71.260 55.459 1.00 13.87
7
ATOM 1489 CA LEUA 196 6.118 71.336 54.215 1.00 13.87
6
ATOM 1490 CB LEUA 196 7.198 70.268 54.118 1.00 13.87
6
ATOM 1491 CG LEUA 196 6.915 68.823 54.431 1.00 13.87
6
ATOM 1492 CD1 LEUA 196 8.258 68.110 54.425 1.00 13.87
6
ATOM 1493 CD2 LEUA 196 6.243 68.678 55.787 1.00 13.87
6
ATOM 1494 C LEUA 196 5.203 71.269 53.046 1.00 13.87
6
ATOM 1495 O LEUA 196 4.114 70.728 53.130 1.00 13.87
8
ATOM 1496 N ARGA 197 5.661 71.821 51.941 1.00 47.25
7
ATOM 1497 CA ARGA 197 4.821 71.899 50.775 1.00 51.20
6
ATOM 1498 CB ARGA 197 4.641 73.395 50.462 1.00 48.70
6
ATOM 1499 CG ARGA 197 4.293 74.237 51.717 1.00 48.34
6
ATOM 1500 CD ARGA 197 3.537 75.533 51.379 1.00 47.12
6
ATOM 1501 NE ARGA 197 4.399 76.676 51.091 1.00 46.27
7
ATOM 1502 CZ ARGA 197 3.991 77.799 50.504 1.00 46.69
6
ATOM 1503 NH1 ARGA 197 2.733 77.943 50.128 1.00 46.72
7
27

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1504 NH2 ARG A197 4.842 78.793 50.303 1.00 46.95
7
ATOM 1505 C ARG A197 5.201 71.099 49.512 1.00 52.55
6
ATOM 1506 O ARG A197 4.357 70.908 48.627 1.00 52.73
8
ATOM 1507 N ILE A198 6.433 70.595 49.435 1.00 26.26
7
ATOM 1508 CA ILE A198 6.870 69.860 48.251 1.00 27.90
6
ATOM 1509 CB ILE A198 6.540 68.363 48.304 1.00 33.44
6
ATOM 1510 CG2 ILE A198 6.900 67.725 46.961 1.00 33.55
6
ATOM 1511 CG1 ILE A198 7.342 67.658 49.403 1.00 33.58
6
ATOM 1512 CD1 ILE A198 6.944 68.011 50.806 1.00 36.15
6
ATOM 1513 C ILE A198 6.162 70.441 47.045 1.00 29.28
6
ATOM 1514 O ILE A198 5.218 69.867 46.534 1.00 28.51
8
ATOM 1515 N TRP A199 6.616 71.608 46.620 1.00 70.20
7
ATOM 1516 CA TRP A199 6.053 72.300 45.476 1.00 74.84
6
ATOM 1517 CB TRP A199 6.808 73.591 45.261 1.00 66.80
6
ATOM 1518 CG TRP A199 5.977 74.760 44.961 1.00 67.13
6
ATOM 1519 CD2 TRP A199 6.068 76.032 45.592 1.00 67.77
6
ATOM 1520 CE2 TRP A199 5.173 76.891 44.927 1.00 68.77
6
ATOM 1521 CE3 TRP A199 6.827 76.534 46.656 1.00 67.48
6
ATOM 1522 CD1 TRP A199 5.051 74.881 43.971 1.00 67.47
6
ATOM 1523 NE1 TRP A199 4.563 76.162 43.938 1.00 68.79
7
ATOM 1524 CZ2 TRP A199 5.013 78.236 45.293 1.00 69.60
6
ATOM 1525 CZ3 TRP A199 6.672 77.862 47.018 1.00 68.06
6
ATOM 1526 CH2 TRP A199 5.773 78.700 46.339 1.00 69.07
6
ATOM 1527 C TRP A199 6.181 71.445 44.222 1.00 77.11
6
ATOM 1528 0 TRP A199 6.307 70.227 44.299 1.00 78.95
8
ATOM 1529 N THR A200 6.179 72.120 43.075 1.00 59.51
7
ATOM 1530 CA THR A200 6.264 71.507 41.746 1.00 61.97
6
ATOM 1531 CB THR A200 6.925 72.484 40.735 1.00108.21
6
ATOM 1532 OG1 THR A200 6.202 73.720 40.724 1.00108.86
8
ATOM 1533 CG2 THR A200 6.921 71.898 39.330 1.00109.30
6
ATOM 1534 C THR A200 6.990 70.163 41.638 1.00 61.64
6
ATOM 1535 0 THR A200 7.902 69.851 42.413 1.00 61.28
8
ATOM 1536 N ASP A201 6.569 69.368 40.661 1.00 26.09
7
ATOM 1537 CA ASP A201 7.185 68.082 40.413 1.00 27.98
6
ATOM 1538 CB ASP A201 6.372 66.988 41.076 1.00 68.36
6
ATOM 1539 CG ASP A201 6.389 67.104 42.566 1.00 71.72
6
ATOM 1540 OD1 ASP A201 5.887 68.124 43.072 1.00 73.07
8
ATOM 1541 OD2 ASP A201 6.903 66.181 43.232 1.00 73.40
8
ATOM 1542 C ASP A201 7.372 67.772 38.922 1.00 29.33
6
ATOM 1543 0 ASP A201 6.637 68.285 38.057 1.00 29.41
8
ATOM 1544 N GLY A202 8.379 66.952 38.621 1.00 97.53
7
ATOM 1545 CA GLY A202 8.616 66.570 37.247 1.00 98.37
6
ATOM 1546 C GLY A202 7.383 65.788 36.850 1.00 99.53
. 6
ATOM 1547 0 GLY A202 6.434 66.341 36.283 1.00 99.89
8
ATOM 1548 N SER A203 7.382 64.501 37.187 1.00102.96
7
ATOM 1549 CA SER A203 6.258 63.625 36.882 1.00101.80
6
ATOM 1550 CB SER A203 6.750 62.365 36.168 1.00 86.92
6
ATOM 1551 OG SER A203 7.569 61.575 37.014 1.00 84.76
8
ATOM 1552 C SER A203 5.495 63.228 38.145 1.00101.29
6
ATOM 1553 0 SER A203 4.282 63.427 38.219 1.00101.42
8
ATOM 1554 N VAL A204 6.212 62.687 39.133 1.00 55.39
7
ATOM 1555 CA VAL A204 5.602 62.243 40.385 1.00 53.33
6
ATOM 1556 CB VAL A204 6.618 61.599 41.316 1.00 24.32
6
ATOM 1557 CG1 VAL A204 7.507 60.687 40.546 1.00 23.40
6
ATOM 1558 CG2 VAL A204 7.383 62.654 42.044 1.00 23.67
6
ATOM 1559 C VAL A204 4.858 63.288 41.210 1.00 52.68
6
28

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1560 0 VAL A 204 .4.943 64.493 40:965 1.00 52.37
ATOM 1561 N THR A 205 4.151 62.786 42.217 1.00 48.41
ATOM 1562 CA THR A 205 3.329 63.592 43.095 1.00 48.06
ATOM 1563 CB THR A 205 2.014 62.892 43.336 1.00 81.95
ATOM 1564 OG1 THR A 205 1.553 62.352 42.094 1.00 84.75
ATOM 1565 CG2 THR A 205 0.983 63.861 43.882 1.00 82.63
ATOM 1566 C THR A 205 3.979 63.857 44.434 1.00 46.17
ATOM 1567 0 THR A 205 4.649 62.995 44.989 1.00 45.62
ATOM 1568 N PRO A 206 3.767 65.065 44.980 1.00 35.54
ATOM 1569 CD PRO A 206 3.005 66.193 44.404 1.00 15.27
ATOM 1570 CA PRO A 206 4.339 65.450 46.265 1.00 36.44
ATOM 1571 CB PRO A 206 3.639 66.763 46.551 1.00 14.81
ATOM 1572 CG PRO A 206 3.532 67.360 45.175 1.00 13.87
ATOM 1573 C PRO A 206 4.128 64.415 47.349 1.00 36.97
ATOM 1574 0 PRO A 206 5.055 64.086 48.074 1.00 36.90
ATOM 1575 N LEU A 207 2.912 63.898 47.461 1.00 83.40
ATOM 1576 CA LEU A 207 2.622 62.891 48.473 1.00 85.60
ATOM 1577 CB LEU A 207 1.172 62.415 48.362 1.00141.23
ATOM 1578 CG LEU A 207 0.790 61.243 49.272 1.00141.75
ATOM 1579 CD1 LEU A 207 1.127 61.575 50.723 1.00141.73
ATOM 1580 CD2 LEU A 207 -0.690 60.937 49.107 1.00142.22
ATOM 1581 C LEU A 207 3.566 61.712 48.284 1.00 85.87
ATOM 1582 0 LEU A 207 3.850 60.975 49.225 1.00 86.19
ATOM 1583 N GLU A 208 4.046 61.542 47.055 1.00 68.68
ATOM 1584 CA GLU A 208 4.967 60.459 46.736 1.00 68.93
ATOM 1585 CB GLU A 208 4.865 60.109 45.242 1.00 99.32
ATOM 1586 CG GLU A 208 3.421 59.967 44.737 1.00 99.03
ATOM 1587 CD GLU A 208 3.320 59.389 43.328 1.00 99.46
ATOM 1588 OE1 GLU A 208 3.687 58.209 43.142 1.00 99.84
ATOM 1589 OE2 GLU A 208 2.872 60.111 42.406 1.00 99.14
ATOM 1590 C GLU A 208 6.378 60.936 47.103 1.00 68.54
ATOM 1591 0 GLU A 208 7.150 60.215 47.741 1.00 67.68
ATOM 1592 N ALA A 209 6.710 62.159 46.701 1.00 75.76
ATOM 1593 CA ALA A 209 8.007 62.720 47.038 1.00 75.57
ATOM 1594 CB ALA A 209 8.098 64.190 46.570 1.00 13.87
ATOM 1595 C ALA A 209 8.041 62.631 48.560 1.00 75.45
ATOM 1596 0 ALA A 209 8.779 61.831 49.128 1.00 75.66
ATOM 1597 N LEU A 210 7.204 63.438 49.205 1.00 70.26
ATOM 1598 CA LEU A 210 7.089 63.467 50.658 1.00 69.79
ATOM 1599 CB LEU A 210 5.782 64.151 51.057 1.00 48.86
ATOM 1600 CG LEU A 210 5.176 63.720 52.392 1.00 48.50
ATOM 1601 CD1 LEU A 210 6.079 64.139 53.540 1.00 48.16
ATOM 1602 CD2 LEU A 210 3.789 64.321 52.526 1.00 49.32
ATOM 1603 C LEU A 210 7.133 62.071 51.277 1.00 69.96
ATOM 1604 0 LEU A 210 7.604 61.901 52.407 1.00 69.73
ATOM 1605 N ASN A 211 6.627 61.080 50.544 1.00 88.32
.
ATOM 1606 CA ASN A 211 6.612 59.699 51.031 1.00 88.59
ATOM 1607 CB ASN A 211 5.539 58.874 50.302 1.00 54.35
ATOM 1608 CG ASN A 211 4.257 58.734 51.111 1.00 53.18
ATOM 1609 OD1 ASN A 211 3.169 59.092 50.650 1.00 51.67
ATOM 1610 ND2 ASN A 211 4.384 58.209 52.330 1.00 53.78
ATOM 1611 C ASN A 211 7.974 59.063 50.826 1.00 88.51
ATOM 1612 0 ASN A 211 8.679 58.750 51.785 1.00 88.15
ATOM 1613 N GLN A 212 8.335 58.877 49.563 1.00102..24
ATOM 1614 CA GLN A 212 9.617 58.296 49.202 1.00102.01
ATOM 1615 CB GLN A 212 9.923 58.634 47.745 1.00132.93
29

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1616 CG GLN A 212 10.206 57.438 46.870 1.00135.91
6
ATOM 1617 CD GLN A 212 9.778 57.674 45.438 1.00138.12
6
ATOM 1618 OE1 GLN A 212 10.162 58.665 44.816 1.00138.09
8
ATOM 1619 NE2 GLN A .212 8.976 56.762 44.904 1.00139.89
7
ATOM 1620 C GLN A 212 10.666 58.910 50.118 1.00100.76
6
ATOM 1621 0 GLN A 212 11.576 58.234 50.592 1.00100.37
8
ATOM 1622 N ALA A 213 10.507 60.204 50.373 1.00 67.79
7
ATOM 1623 CA ALA A 213 11.420 60.940 51.220 1.00 65.50
6
ATOM 1624 CB ALA A 213 10.995 62.382 51.298 1.00 13.87
6
ATOM 1625 C ALA A 213 11.475 60.333 52.608 1.00 64.69
6
ATOM 1626 0 ALA A 213 12.525 59.856 53.030 1.00 65.22
8
ATOM 1627 N VAL A 214 10.349 60.341 53.317 1.00 68.52
7
ATOM 1628 CA VAL A 214 10.311 59.790 54.673 1.00 69.21
6
ATOM 1629 CB VAL A 214 8.852 59.699 55.228 1.00100.42
6
ATOM 1630 CG1 VAL A 214 8.155 61.040 55.090 1.00101.75
6
ATOM 1631 CG2 VAL A 214 8.069 58.622 54.503 1.00101.44
6
ATOM 1632 C VAL A 214 10.934 58.397 54.694 1.00 68.93
6
ATOM 1633 0 VAL A 214 11.367 57.906 55.738 1.00 69.47
8
ATOM 1634 N ALA A 215 10.986 57.777 53.519 1.00 54.30
7
ATOM 1635 CA ALA A 215 11.531 56.438 53.371 1.00 51.91
6
ATOM 1636 CB ALA A 215 11.193 55.893 51.997 1.00 65.24
6
ATOM 1637 C ALA A 215 13.023 56.442 53.567 1.00 50.60
6
ATOM 1638 0 ALA A 215 13.527 55.844 54.507 1.00 50.82
8
ATOM 1639 N ILE A 216 13.721 57.129 52.671 1.00 43.01
7
ATOM 1640 CA ILE A 216 15.172 57.198 52.722 1.00 41.44
6
ATOM 1641 CB ILE A 216 15.730 58.209 51.708 1.00 19.95
6
ATOM 1642 CG2 ILE A 216 17.099 57.774 51.249 1.00 18.66
6
ATOM 1643 CG1 ILE A 216 14.829 58.282 50.484 1.00 19.48
6
ATOM 1644 CD1 ILE A 216 14.631 56.966 49.787 1.00 21.24
6
ATOM 1645 C ILE A 216 15.663 57.587 54.108 1.00 41.48
6
ATOM 1646 0 ILE A 216 16.557 56.940 54.656 1.00 41.44
8
ATOM 1647 N LEU A 217 15.072 58.635 54.679 1.00 28.89
7
ATOM 1648 CA LEU A 217 15.470 59.104 56.003 1.00 28.88
6
ATOM 1649 CB LEU A 217 14.667 60.346 56.379 1.00 22.95
6
ATOM 1650 CG LEU A 217 14.900 60.934 57.770 1.00 21.45
6
ATOM 1651 CD1 LEU A 217 16.368 60.994 58.127 1.00 20.21
6
ATOM 1652 CD2 LEU A 217 14.311 62.321 57.757 1.00 21.23
6
ATOM 1653 C LEU A 217 15.320 58.037 57.082 1.00 29.41
6
ATOM 1654 0 LEU A 217 16.083 58.010 58.049 1.00 29.86
8
ATOM 1655 N LYS A 218 14.328 57.164 56.913 1.00 50.57
7
ATOM 1656 CA LYS A 218 14.084 56.078 57.859 1.00 51.86
6
ATOM 1657 CB LYS A 218 12.685 55.496 57.679 1.00 57.70
6
ATOM 1658 CG LYS A 218 11.535 56.416 58.036 1.00 59.45
6
ATOM 1659 CD LYS A 218 10.227 55.649 57.956 1.00 61.30
6
ATOM 1660 CE LYS A 218 9.056 56.466 58.459 1.00 63.03
6
ATOM 1661 NZ LYS A 218 7.845 55.607 58.644 1.00 64.61
7
ATOM 1662 C LYS A 218 15.095 54.993 57.567 1.00 51.99.
6
ATOM 1663 0 LYS A 218 15.618 54.363 58.477 1.00 51.13
8
ATOM 1664 N GLU A 219 15.345 54.792 56.275 1.00 59.27
7
ATOM 1665 CA GLU A 219 16.283 53.799 55.766 1.00 61.46
6
ATOM 1666 CB GLU A 219 16.251 53.828 54.233 1.00113.88
6
ATOM 1667 CG GLU A 219 16.891 52.640 53.528 1.00117.59
6
ATOM 1668 CD GLU A 219 16.680 52.676 52.011 1.00120.81
6
ATOM 1669 OE1 GLU A 219 15.515 52.611 51.550 1.00121.78
8
ATOM 1670 OE2 GLU A 219 17.685 52.770 51.273 1.00122.09
8
ATOM 1671 C GLU A 219 17.689 54.110 56.285 1.00 60.81
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1672 O GLU A 219 18.427 53.215 56.706 1.00 60.38
ATOM 1673 N HIS A 220 18.054 55.386 56.262 1.00 65.87
ATOM 1674 CA HIS A 220- 19.359 55.800 56.741 1.00 65.62
ATOM 1675 CB HIS A 220 19.667 57.206 56.282 1.00 39.54
ATOM 1676 CG HIS A 220 19.896 57.301 54.816 1.00 38.27
ATOM 1677 CD2 HIS A 220 19.095 57.728 53.815 1.00 37.65
ATOM 1678 ND1 HIS A 220 21.047 56.838 54.218 1.00 37.62
ATOM 1679 CE1 HIS A 220 20.942 56.973 52.909 1.00 37.73
ATOM 1680 NE2 HIS A 220 19.766 57.510 52.639 1.00 37.89
ATOM 1681 C HIS A 220 19.377 55.752 58.237 1.00 65.89
ATOM 2682 O HIS A 220 20.261 55.148 58.828 1.00 67.49
ATOM 1683 N LEU A 221 18.396 56.397 58.853 1.00 48.36
ATOM 1684 CA LEU A 221 18.305 56.408 60.304 1.00 48.22
ATOM 1685 CB LEU A 221 16.867 56.693 60.721 1.00 31.54
ATOM 1686 CG LEU A 221 ~ 16.566 57.975 61.495 1.00 30.44
ATOM 1687 CD1 LEU A 221 17.607 58.127 62.603 1.00 30.22
ATOM 1688 CD2 LEU A 221 16.553 59.181 60.566 1.00 29.38
ATOM 1689 C LEU A 221 18.736 55.047 60.862 1.00 48.73
ATOM 1690 0 LEU A 221 19.479 54.960 61.849 1.00 48.06
ATOM 1691 N ASN A 222 18.267 53.990 60.199 1.00 57.57
ATOM 1692 CA ASN A 222 18.561 52.618 60.594 1.00 58.23
ATOM 1693 CB ASN A 222 18.084 51.638 59.523 1.00 62.29
ATOM 1694 CG ASN A 222 16.568 51.594 59.405 1.00 62.92
ATOM 1695 OD1 ASN A 222 15.856 51.358 60.386 1.00 63.28
ATOM 1696 ND2 ASN A 222 16.067 51.816 58.199 1.00 63.76
ATOM 1697 C ASN A 222 20.032 52.423 60.828 1.00 58.36
ATOM 1698 0 ASN A 222 20.447 52.071 61.926 1.00 58.19
ATOM 1699 N TYR A 223 20.810 52.667 59.782 1.00 57.73
ATOM 1700 CA TYR A 223 22.261 52.520 59.819 1.00 58.14
ATOM 1701 CB TYR A 223 22.914 53.522 58.857 1.00 81.51
ATOM 1702 CG TYR A 223 22.622 53.233 57.395 1.00 81.37
ATOM 1703 CD1 TYR A 223 22.759 51.944 56.883 1.00 81.65
ATOM 1704 CE1 TYR A 223 22.503 51.670 55.549 1.00 82.13
ATOM 1705 CD2 TYR A 223 22.221 54.245 56.525 1.00 80.64
ATOM 1706 CE2 TYR A 223 21.963 53.980 55.189 1.00 81.03
ATOM 1707 CZ TYR A 223 22.105 52.691 54.708 1.00 81.92
ATOM 1708 OH TYR A 223 21.842 52.412 53.386 1.00 82.77
ATOM 1709 C TYR A 223 22.911 52.622 61.197 1.00 58.19
ATOM 1710 0 TYR A 223 23.891 51.921 61.467 1.00 58.31
ATOM 1711 N PHE A 224 22.388 53.482 62.067 1.00 62.07
ATOM 1712 CA PHE A 224 22.964 53.592 63.403 1.00 63.51
ATOM 1713 CB PHE A 224 22.478 54.865 64.119 1.00 39.72
ATOM 1714 CG PHE A 224 22.643 56.148 63.319 1.00 36.56
ATOM 1715 CD1 PHE A 224 21.870 56.389 62.183 1.00 35.76
ATOM 1716 CD2 PHE A 224 23.518 57.139 63.743 1.00 34.63
ATOM 1717 CE1 PHE A 224 21.967 57.602 61.490 1.00 34.55
ATOM 1718 CE2 PHE A 224 23.616 58.340 63.057 1.00 34.38
ATOM 1729 CZ PHE A 224 22.837 58.573 61.929 1.00 33.84
ATOM 1720 C PHE A 224 22.505 52.342 64.177 1.00 64.88
ATOM 1721 0 PHE A 224 21.931 52.437 65.266 1.00 64.71
ATOM 1722 N ALA A 225 22.773 51.173 63.598 1.00 57.96
ATOM 1723 CA ALA A 225 22.370 49.899 64.179 1.00 60.31
ATOM 1724 CB ALA A 225 21.893 48.968 63.074 1.00 89.79
ATOM 1725 C ALA A 225 23.424 49.192 65.022 1.00 61.98
ATOM 1726 0 ALA A 225 23.407 49.299 66.244 1.00 62.94
ATOM 1727 N ASN A 226 24.324 48.451 64.376 1.00 79.27
31

CA 02440409 2003-09-09
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ATOM 1728 CA ASN A 226 25.369 47.711 65.092 1.00 80.93
ATOM 1729 CB ASN A 226 25.399 46.255 64.621 1.00184.67
ATOM 1730 CG ASN A 226 24.239 45.444 65.168 1.00186.86
ATOM 1731 OD1 ASN A 226 24.046 44.287 64.793 1.00187.92
ATOM 1732 ND2 ASN A 226 23.463 46.045 66.066 1.00187.67
ATOM 1733 C ASN A 226 26.759 48.329 64.969 1.00 80.54
ATOM 1734 O ASN A 226 27.355 48.365 63.892 1.00 80.25
ATOM 1735 N PRO A 227 27.301 48.806 66.095 1.00 31.83
ATOM 1736 CD PRO A 227 26.576 48.977 67.370 1.00 64.99
ATOM 1737 CA PRO A 227 28.614 49.440 66.160 1.00 32.33
ATOM 1738 CB PRO A 227 28.393 50.513 67.207 1.00 64.99
ATOM 1739 CG PRO A 227 27.572 49.753 68.225 1.00 64.42
ATOM 1740 C PRO A 227 29.736 48.501 66.549 1.00 33.14
ATOM 1741 O PRO A 227 29.510 47.554 67.297 1.00 32.x,56
ATOM 1742 N GLU A 228 30.944 48.779 66.054 1.00 45.58
ATOM 1743 CA GLU A 228 32.119 47.962 66.368 1.00 47.66
ATOM 1744 CB GLU A 228 32.962 47.714 65.112 1.00122.21
ATOM 1745 CG GLU A 228 32.226 47.022 63.975 1.00125.27
ATOM 1746 CD GLU A 228 33.048 46.960 62.698 1.00126.99
ATOM 1747 OE1 GLU A 228 33.469 48.027 62.204 1.00127.85
ATOM 1748 OE2 GLU A 228 33.272 45.846 62.184 1.00128.27
ATOM 1749 C GLU A 228 32.961 48.719 67.387 1.00 47.81
ATOM 1750 O GLU A 228 32.563 48.761 68.572 1.00 48.48
ATOM 1751 OXT GLU A 228 34.003 49.278 66.984 1.00122.18
ATOM 1752 CB ALA B 3 36.891 46.742 48.658 1.00177.12
ATOM 1753 C ALA B 3 35.150 46.906 50.443 1.00140.19
ATOM 1754 O ALA B 3 35.794 46.246 51.264 1.00140.05
ATOM 1755 N ALA B 3 34.714 47.787 48.149 1.00139.84
ATOM 1756 CA ALA B 3 35.397 46.724 48.947 1.00139.99
ATOM 1757 N SER B 4 34.224 47.811 50.775 1.00101.63
ATOM 1758 CA SER B 4 33.825 48.123 52.156 1.00100.90
ATOM 1759 CB SER B 4 34.990 47.939 53.138 1.00153.10
ATOM 1760 OG SER B 4 35.096 46.587 53.558 1.00154.08
ATOM 1761 C SER B 4 33.278 49.539 52.305 1.00 99.84
ATOM 1762 O SER B 4 34.037 50.510 52.324 1.00100.06
ATOM 1763 N ALA B 5 31.956 49.630 52.423 1.00120.11
ATOM 1764 CA ALA B 5 31.232 50.889 52.586 1.00119.35
ATOM 1765 CB ALA B 5 31.983 52.033 51.925 1.00 90.01
ATOM 1766 C ALA B 5 29.853 50.744 51.955 1.00118.60
ATOM 1767 O ALA B 5 28.836 50.697 52.649 1.00118.65
ATOM 1768 N ALA B 6 29.845 50.667 50.627 1.00101.80
ATOM 1769 CA ALA B 6 28.631 50.519 49.829 1.00101.01
ATOM 1770 CB ALA B 6 28.490 49.062 49.369 1.00 18.92
ATOM 1771 C ALA B 6 27.351 50.984 50.526 1.00100.19
ATOM 1772 0 ALA B 6 27.349 51.981 51.249 1.00101.16
ATOM 1773 N LYS B 7 26.261 50.260 50.293 1.00113.51
ATOM 1774 CA LYS B 7 24.971 50.601 50.878 1.00111:85
ATOM 1775 CB LYS B 7 23.841 50.078 49.985 1.00206.39
ATOM 1776 CG LYS B 7 23.851 50.697 48.595 1.00208.87
ATOM 1777 CD LYS B 7 22.788 50.116 47.670 1.00208.87
ATOM 1778 CE LYS B 7 22.858 50.7.83 46.293 1.00208.87
ATOM 17-79 NZ LYS B 7 21.902 50.212 45.298 1.00208.87
ATOM 1780 C LYS B 7 24.821 50.048 52.285 1.00109.93
ATOM 17.81 0 LYS B 7 23.808 49.435 52.615 1.00110.05
ATOM 1782 N ALA B 8 25.835 50.272 53.114 1.00 61.60
ATOM 1783 CA ALA B 8 25.815 49.803 54.492 1.00 58.50
32

CA 02440409 2003-09-09
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ATOM 1784 CB ALA B 8 25.895 48.298 54.521 1.00 56.84 6
ATOM 1785 C ALA B 8 26.989 50.402 55.254 1.00 56.66 6
ATOM 1786 O ALA B 8 28.016 49.749 55.439 1.00 56.49 8
ATOM 1787 N PRO B 9 26.847 51.654 55.717 1.00 53.70 7
ATOM 1788 CD PRO B 9 25.625 52.472 55.654 1.00 64.50 6
ATOM 1789 CA PRO B 9 27.898 52.354 56.460 1.00 51.92 6
ATOM 1790 CB PRO B 9 27.319 53.753 56.635 1.00 63.49 6
ATOM 1791 CG PRO B 9 25.857 53.478 56.754 1.00 64.27 6
ATOM 1792 C PRO B 9 28.239 51.697 57.784 1.00 51.30 6
ATOM 1793 0 PRO B 9 27.392 51.053 58.400 1.00 51.69 8
ATOM 1794 N VAL B 10 29.487 51.878 58.207 1.00 51.49 7'
ATOM 1795 CA VAL B 10 29.983 51.312 59.450 1.00 50.52 6
ATOM 1796 CB VAL B 10 31.460 50.948 59.346 1.00 58.34 6
ATOM 1797 CG1 VAL B 10 31.892 50.206 60.596 1.00 58.83 6
ATOM 1798 CG2 VAL B 10 31.705 50.121 58.100 1.00 58.45 6
ATOM 1799 C VAL B 10 29.846 52.297 60.590 1.00 50.72 6
ATOM 1800 0 VAL B 10 30.563 53.298 60.636 1.00 51.63 8
ATOM 1801 N PHE B 11 28.929 51.986 61.505 1.00 53.80 7
ATOM 1802 CA PHE B 11 28.627 52.789 62.693 1.00 52.66 6
ATOM 1803 CB PHE B 11 27.231 52.403 63.167 1.00 77.05 6
ATOM 1804 CG PHE B 11 26.651 53.330 64.173 1.00 77.97 6
ATOM 1805 CD1 PHE B 11 26.463 54.671 63.869 1.00 78.45 6
ATOM 1806 CD2 PHE B 11 26.251 52.855 65.418 1.00 79.06 6
ATOM 1807 CE1 PHE B 11 25.883 55.527 64.786 1.00 78.89 6
ATOM 1808 CE2 PHE B 11 25.667 53.704 66.346 1.00 79.40 6
ATOM 1809 CZ PHE B 11 25.482 55.044 66.029 1.00 79.32 6
ATOM 1810 C PHE B 11 29.673 52.486 63.786 1.00 53.13 6
ATOM 1811 0 PHE B 11 29.660 51.407 64.379 1.00 53.82 8
ATOM 1812 N THR B 12 30.575 53.429 64.056 1.00 66.78 7
ATOM 1813 CA THR B 12 31.636 53.207 65.047 1.00 67.68 6
ATOM 1814 CB THR B 12 33.026 53.500 64.437 1.00114.71 6
ATOM 1815 OG1 THR B 12 33.244 52.650 63.305 1.00115.14 8
ATOM 1816 CG2 THR B 12 34.120 53.252 65.460 1.00115.46 6
ATOM 1817 C THR B 12 31.501 54.033 66.323 1.00 68.21 6
ATOM 1818 0 THR B 12 31.599 55.258 66.286 1.00 68.13 8
ATOM 1819 N ALA B 13 31.315 53.351 67.454 1.00 70.17 7
ATOM 1820 CA ALA B 13 31.151 54.002 68.760 1.00 71.26 6
ATOM 1821 CB ALA B 13 30.227 53.173 69.630 1.00 41.04 6
ATOM 1822 C ALA B 13 32.459 54.233 69.502 1.00 73.13 6
ATOM 1823 0 ALA B 13 33.526 53.838 69.038 1.00 74.07 8
ATOM 1824 N THR B 14 32.371 54.867 70.668 1.00 89.91 7
ATOM 1825 CA THR B 14 33.561 55.127 71.473 1.00 91.34 6
ATOM 1826 CB THR B 14 34.018 56.582 71.335 1.00149.30 6
ATOM 1827 OG1 THR B 14 34.368 56.838 69.968 1.00151.79 8
ATOM 1828 CG2 THR B 14 35.232 56.852 72.214 1.00148.89 6
ATOM 1829 C THR B 14 33.351 54.802 72.946 1.00 93.15 6
ATOM 1830 0 THR B 14 34.305 54.731 73.713 1.00 93.29 8
ATOM 1831 N THR B 15 32.095 54.612 73.332 1.00108.52 7
ATOM 1832 CA THR B 15 31.741 54.251 74.701 1.00109.63 6
ATOM 1833 CB THR B 15 31.501 52.723 74.807 1.00158.82 6
ATOM 1834 OG1 THR B 15 32.586 52.022 74.187 1.00160.26 8
ATOM 1835 CG2 THR B 15 30.196 52.331 74.119 1.00158.82 6
ATOM 1836 C THR B 15 32.739 54.657 75.791 1.00110.48 6
ATOM 1837 0 THR B 15 33.354 53.804 76.432 1.00111.01 8
ATOM 1838 N GLN B 16 32.893 55.959 76.008 1.00 86.50 7
ATOM 1839 CA GLN B 16 33.806 56.463 77.034 1.00 87.15 6
33

CA 02440409 2003-09-09
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ATOM 1840 CB GLN B 16 33.877 57.996 76.949 1.00 90.84
ATOM 1841 CG GLN B 16 34.756 58.676 77.985 1.00 92.61
ATOM 1842 CD GLN B 16 36.183 58.158 77.984 1.00 94.30
ATOM 1843 OE1 GLN B 16 36.836 58.104 76.941 1.00 96.12
ATOM 1844 NE2 GLN B 16 36.679 57.783 79.159 1.00 95.03
ATOM 1845 C GLN B 16 33.307 56.022 78.414 1.00 88.26
ATOM 1846 O GLN B 16 33.879 56.382 79.442 1.00 88.50
ATOM 1847 N GLY B 1:7 32.237 55.232 78.426 1.00144.12
ATOM 1848 CA GLY B 17 31.684 54.764 79.682 1.00143.78
ATOM 1849 C GLY B 17 30.395 55.477 80.037 1.00144.22
ATOM 1850 O GLY B 17 29.395 55.356 79.332 1.00144.77
ATOM 1851 N ASP B 18' 30.413 56.231 81.128 1.00104.50
ATOM 1852 CA ASP B 18 29.223 56.951 81.561 1.00103.73
ATOM 1853 CB ASP B 18 29.371 57.379 83.021 1.00208.05
ATOM 1854 CG ASP B 18 30.681 58.084 83.288 1.00208.87
ATOM 1855 OD1 ASP B 18 31.740 57.435 83.153 1.00208.87
ATOM 1856 OD2 ASP B 18 30.651 59.284 83.632 1.00208.87
ATOM 1857 C ASP B 18 28.871 58.164 80.702 1.00102.50
ATOM 1858 O ASP B 18 27.779 58.714 80.836 1.00102.98
ATOM 1859 N HIS B 19 29.792 58.590 79.836 1.00 46.05
ATOM 1860 CA HIS B 19 29.548 59.727 78.943 1.00 44.55
ATOM 1861 CB HIS B 19 29.669 61.056 79.703 1.00142.23
ATOM 1862 CG HIS B 19 30.981 61.256 80.394 1.00144.41
ATOM 1863 CD2 HIS B 19 32.128 60.536 80.370 1.00145.87
ATOM 1864 ND1 HIS B 19 31.221 62.328 81.225 1.00145.49
ATOM 1865 CE1 HIS B 19 32.457 62.262 81.684 1.00146.38
ATOM 1866 NE2 HIS B 19 33.029 61.183 81.179 1.00146.80
ATOM 1867 C HIS B 19 30.449 59.743 77.705 1.00 42.86
ATOM 1868 O HIS B 19 31.104 58.758 77.389 1.00 41.54
ATOM 1869 N TYR B 20 30.464 60.864 76.998 1.00101.12
ATOM 1870 CA TYR B 20 31.275 61.004 75.793 1.00 98.97
ATOM 1871 CB TYR B 20 32.761 61.154 76.146 1.00129.01
ATOM 1872 CG TYR B 20 33.590 61.769 75.031 1.00130.15
ATOM 1873 CD1 TYR B 20 34.959 61.990 75.189 1.00130.78
ATOM 1874 CE1 TYR B 20 35.718 62.591 74.171 1.00131.57
ATOM 1875 CD2 TYR B 20 32.997 62.160 73.827 1.00130.28
ATOM 1876 CE2 TYR B 20 33.740 62.759 72.808 1.00130.52
ATOM 1877 CZ TYR B 20 35.099 62.974 72.981 1.00130.95
ATOM 1878 OH TYR B 20 35.825 63.575 71.966 1.00129.42
ATOM 1879 C TYR B 20 31.106 59.821 74.849 1.00 96.88
ATOM 1880 O TYR B 20 32.029 59.035 74.654 1.00 97.42
ATOM 1881 N GLY B 21 29.924 59.702 74.261 1.00 94.58
ATOM 1882 CA GLY B 21 29.669 58.615 73.339 1.00 91.23
ATOM 1883 C GLY B 21 30.045 58.982 71.918 1.00 90.19
ATOM 1884 0 GLY B 21 29.182 59.139 71.058 1.00 90.24
ATOM 1885 N GLU B 22 31.342 59.124 71.676 1.00 82.21
ATOM 1886 CA GLU B 22 31.865 59.467 70.356 1.00 79.42
ATOM 1887 CB GLU B 22 33.386 59.547 70.456 1.00 80.54
ATOM 1888 CG GLU B 22 34.110 60.342 69.407 1.00 81.39
ATOM 1889 CD GLU B 22 35.596 60.408 69.716 1.00 82.92
ATOM 1890 OE1 GLU B 22 36.274 59.369 69.576 1.00 82.42
ATOM 1891 OE2 GLU B 22 36.086 61.489 70.118 1.00 84.93
ATOM 1892 C GLU B 22 31.438 58.371 69.368 1.00 77.68
ATOM 1893 O GLU B 22 31.832 57.211 69.502 1.00 77.30
ATOM 1894 N PHE B 23 30.617 58.736 68.387 1.00 48.34
ATOM 1895 CA PHE B 23 30.125 57.766 67.405 1.00 45.62
34

CA 02440409 2003-09-09
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ATOM 1896 CB PHE B 23 28.607 57.648 67.541 1.00 54.52
6
ATOM 1897 CG PHE B 23 28.164 57.240 68.920 1.00 53.70
6
ATOM 1898 CD1 PHE B 23 26.861 57.435 69.330 1.00 54.29
6
ATOM 1899 CD2 PHE B 23 29.065 56.674 69.816 1.00 52.65
6
ATOM 1900 CE1 PHE B 23 26.458 57.077 70.611 1.00 55.28
6
ATOM 1901 CE2 PHE B 23 28.672 56.314 71.098 1.00 53.37
6
ATOM 1902 CZ PHE B 23 27.368 56.515 71.497 1.00 54.11
6
ATOM 1903 C PHE B 23 30.538 58.118 65.978 1.00 44.20
6
ATOM 1904 0 PHE B 23 30.849 59.265 65.688 1.00 44.77
8
ATOM 1905 N VAL B 24 30.534 57.139 65.082 1.0_044.68
7
ATOM 1906 CA VAL B 24 30.993 57.388 63.726 1.00 43.27
6
ATOM 1907 CB VAL B 24 32.529 57.187 63.625 1.00 15.18
6
ATOM 1908 CG1 VAL B 24 32.988 57.381 62.216 1.00 13.87
6
ATOM 1909 CG2 VAL B 24 33.248 58.131 64.528 1.00 15.68
6
ATOM 1910 C VAL B 24 30.395 56.492 62.656 1.00 44.28
6
ATOM 1911 0 VAL B 24 30.974 55.449 62.353 1.00 44.86
8
ATOM 1912 N LEU B 25 29.253 56.865 62.081 1.00 60.79
7
ATOM 1913 CA LEU B 25 28.698 56.055 60.994 1.00 60.09
6
ATOM 1914 CB LEU B 25 27.172 56.238 60.872 1.00 15.01
6
ATOM 1915 CG LEU B 2~ 26.568 57.265 59.920 1.00 13.87
6
ATOM 1916 CD1 LEU B 25 26.903 56.857 58.514 1.00 13.87
6
ATOM 1917 CD2 LEU B 25 25.053 57.330 60.085 1.00 13.87
6
ATOM 1918 C LEU B 25 29.476 56.695 59.848 1.00 61.13
6
ATOM 1919 0 LEU B 25 29.722 57.903 59.889 1.00 60.97
8
ATOM 1920 N GLU B 26 29.866 55.924 58.832 1.00 89.90
7
ATOM 1921 CA GLU B 26 30.705 56.519 57.794 1.00 91.26
6
ATOM 1922 CB GLU B 26 32.037 55.774 57.778 1.00102.03
6
ATOM 1923 CG GLU B 26 33.175 56.601 57.229 1.00104.07
6
ATOM 1924 CD GLU B 26 34.422 55.782 57.030 1.00107.14
6
ATOM 1925 OE1 GLU B 26 34.388 54.866 56.182 1..00109.13
8
ATOM 1926 OE2 GLU B 26 35.428 56.044 57.724 1.00107.71
8
ATOM 1927 C GLU B 26 30.263 56.838 56.348 1.00 90.05
6
ATOM 1928 O GLU B 26 29.431 57.728 56.157 1.00 90.37
8
ATOM 1929 N PRO B 27 30.785 56.125 55.317 1.00 56.95
7
ATOM 1930 CD PRO B 27 31.531 54.861 55.190 1.00 13.87
6
ATOM 1931 CA PRO B 27 30.309 56.550 53.993 1.00 58.52
6
ATOM 1932 CB PRO B 27 30.825 55.449 53.056 1.00 14.57
6
ATOM 1933 CG PRO B 27 32.052 54.963 53.766 1.00 13.87
6
ATOM 1934 C PRO B 27 28.822 56.836 53.851 1.00 58.78
6
ATOM 1935 0 PRO B 27 27.965 55.972 54.047 1.00 59.53
8
ATOM 1936 N LEU B 28 28.536 58.086 53.519 1.00 37.49
7
ATOM 1937 CA LEU B 28 27.174 58.550 53.347 1.00 38.26
6
ATOM 1938 CB LEU B 28 26.741 59.309 54.611 1.00 21.14
6
ATOM 1939 CG LEU B 28 26.710 58.546 55.951 1.00 18.35
6
ATOM 1940 CD1 LEU B 28 26.465 59.481 57.103 1.00 18.15
6
ATOM 1941 CD2 LEU B 28 25.622 57.519 55.898 1.00 17.94
6
ATOM 1942 C LEU B 28 27.232 59.479 52.150 1.00 39.15
6
ATOM 1943 0 LEU B 28 28.181 60.234 52.018 1.00 39.68
8
ATOM 1944 N GLU B 29 26.246 59.418 51.267 1.00 28.57
7
ATOM 1945 CA GLU B 29 26.255 60.294 50.095 1.00 30.85
6
ATOM 1946 CB GLU B 29 25.014 60.038 49.235 1.00193.66
6
ATOM 1947 CG GLU B 29 25.037 60.724 47.871 1.00198.20
6
ATOM 1948 CD GLU B 29 26.068 60.131 46.927 1.00200.49
6
ATOM 1949 OE1 GLU B 29 27.268 60.137 47.276 1.00202.11
8
ATOM 1950 OE2 GLU B 29 25.677 59.659 45.835 1.00199.88
8
ATOM 1951 C GLU B 29 26.296 61.769 50.530 1.00 29.83
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 1952 O GLU B 29 25.524 62.200 51.405 1.00 28.53 8
ATOM 1953 N ARG B 30 27.183 62.548 49.915 1.00 47.67 7
ATOM 1954 CA ARG B 30 27.313 63.950 50.295 1.00 48.20 6
ATOM 1955 CB ARG B 30 28.083 64.749 49.228 1.00 85.66 6
ATOM 1956 CG ARG B 30 27.338 64.986 47.926 1.00 89.54 6
ATOM 1957 CD ARG B 30 27.587 66.396 47.389 1.00 92.43 6
ATOM 1958 NE ARG B 30 26.693 66.724 46.275 1.00 95.87 7
ATOM 1959 CZ ARG B 30 26.707 67.874 45.602 1.00 96.56 6
ATOM 1960 NH1 ARG B 30 27.571 68.830 45.922 1.00 96.82 7
ATOM 1961 NH2 ARG B 30 25.863 68.065 44.597 1.00 96.67 7
ATOM 1962 C ARG B 30 25.958 64.596 50.553 1.00 45.28 6
ATOM 1963 O ARG B 30 25.218 64.905 49.629 1.00 44.63 8
ATOM 1964 N GLY B 31 25.619 64.788 51.816 1.00 66.33 7
ATOM 1965 CA GLY B 31 24.349 65.420 52.101 1.00 66.30 6
ATOM 1966 C GLY B 31 23.482 64.622 53.040 1.00 65.00 6
~
ATOM 1967 O GLY B 31 22.256 64.592 52.895 1.00 65.26 8
ATOM 1968 N PHE B 32 24.120 63.962 53.996 1.00 58.11 7
ATOM 1969 CA PHE B 32 23.403 63.171 54.977 1.00 55.81 6
ATOM 1970 CB PHE B 32 23.260 61.722 54.506 1.00 24.56 6
ATOM 1971 CG PHE B 32 22.180 61.538 53.483 1.00 21.18 6
ATOM 1972 CD1 PHE B 32 20.874 61.282 53.877 1.00 20.75 6
ATOM 1973 CD2 PHE B 32 22.451 61.701 52.126 1.00 19.17 6
ATOM 1974 CE1 PHE B 32 19.853 61.199 52.946 1.00 19.26 6
ATOM 1975 CE2 PHE B 32 21.431 61.623 51.180 1.00 17.56 6
ATOM 1976 CZ PHE B 32 20.131 61.371 51.592 1.00 18.01 6
ATOM 2977 C PHE B 32 24.156 63.259 5&.277 1.00 55.28 6
ATOM 1978 O PHE B 32 23.590 63.048 57.347 1.00 56.33 8
ATOM 1979 N GLY B 33 25.439 63.580 56.189 1.00 33.58 7
ATOM 1980 CA GLY B 33 26.199 63.727 57.408 1.00 34.02 6
ATOM 1981 C GLY B 33 25.458 64.819 58.154 1.00 33.98 6
ATOM 1982 0 GLY B 33 25.449 64.881 59.384 1.00 33.68 8
ATOM 1983 N VAL B 34 24.820 65.690 57.376 1.00 53.79 7
ATOM 1984 CA VAL B 34 24.043 66.790 57.919 1.00 53.45 6
ATOM 1985 CB VAL B 34 23.890 67.945 56.906 1.00 33.83 6
ATOM 1986 CG1 VAL B 34 23.298 69.148 57.586 1.00 35.07 6
ATOM 1987 CG2 VAL B 34 25.219 68.294 56.294 1.00 35.07 6
ATOM 1988 C VAL B 34 22.658 66.236 58.180 1.00 53.28 6
ATOM 1989 O VAL B 34 22.196 66.197 59.321 1.00 53.53 8
ATOM 1990 N THR B 35 22.023 65.781 57.100 1.00 54.49 7
ATOM 1991 CA THR B 35 20.665 65.249 57.137 1.00 53.19 6
ATOM 1992 CB THR B 35 20.323 64.431 55.896 1.00 47.42 6
ATOM 1993 OG1 THR B 35 20.965 64.990 54.751 1.00 47.14 8
ATOM 1994 CG2 THR B 35 18.832 64.472 55.663 1.00 47.59 6
ATOM 1995 C THR B 35 20.379 64.382 58.336 1.00 52.60 6
ATOM 1996 O THR B 35 19.224 64.223 58.725 1.00 53.16 8
ATOM 1997 N LEU B 36 21.412 63.806 58.926 1.00 20.23 7
ATOM 1998 CA LEU B 36 21.170 62.992 60.092 1.00 20.76 6
ATOM 1999 CB LEU B 36 21.763 61.602 59.919 1.00 46.64 6
ATOM 2000 CG LEU B 36 21.303 60.874 58.663 1.00 46.22 6
ATOM 2001 CD1 LEU B 36 21.674 59.399 58.803 1.00 46.91 6
ATOM 2002 CD2 LEU B 36 19.801 61.046 58.465 1.00 44.40 6
ATOM 2003 C LEU B 36 21.738 63.641 61.332 1.00 21.80 6
~
ATOM 2004 O LEU B 36 20.991 64.098 62.189 1.00 21.82 8
ATOM 2005 N GLY B 37 23.064 63.680 61.413 1.00 70.68 7
ATOM 2006 CA GLY B 37 23.743 64.256 62.558 1.00 71.90 6
ATOM 2007 C GLY B 37 22.994 65.360 63.274 1.00 72.46 6
36

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ATOM 2008 0 GLY B 37 22.953 65.375 64.503 1.00 72.33
8
ATOM 2009 N ASN B 38 22.386 66.277 62.524 1.00 65.39
7
ATOM 2010 CA ASN B 38 21.673 67.384 63.150 1.00 65.26
6
ATOM.2011 CB ASN B 38 21.300 68.446 62.113 1.00 25.63
6
ATOM 2012 CG ASN B 38 21.229 69.838 62.718 1.00 24.58
6
ATOM 2013 OD1 ASN B 38 20.472 70.697 62.258 1.00 26.11
8
ATOM 2014 ND2 ASN B 38 22.036 70.074 63.749 1.00 22.75
7
ATOM 2015 C ASN B 38 20.431 66.983 63.955 1.00 66.04
6
ATQM 2016 0 ASN B 38 20.450 67:067 65.184 1.00 66.24
8
ATOM 2017 N PRO B 39 19.339 66.538 63.284 1.00 86.12
7
ATOM 2018 CD PRO B 39 19.158 66.243 61.850 1.00 27.75
6
ATOM 2019 CA PRO B 39 18.132 66.150 64.023 1.00 86.08
6
ATOM 2020 CB PRO B 39 17.337 65.378 62.985 1.00 27.67
6
ATOM 2021 CG PRO B 39 17.665 66.099 61.737 2.00 26.77
6
ATOM 2022 C PRO B 39 18.429 65.327 65.270 1.00 87.63
6
ATOM 2023 O PRO B 39 17.793 65.515 66.305 1.00 88.84
8
ATOM 2024 N LEU B 40 19.389 64.412 65.170 1.00 37.16
7
ATOM 2025 CA LEU B 40 19.771 63.610 66.327 1.00 38.01
~ 6
ATOM 2026 CB LEU B 40 20.829 62.552 65.964 1.00 30.64
6
ATOM 2027 CG LEU B 40 20.398 61.361 65.106 1.00 30.84
6
ATOM 2028 CD1 LEU B 40 19.168 60.700 65.700 1.00 30.00
6
ATOM 2029 CD2 LEU B 40~ 20.101 61.836 63.705 1.00 31.53
6
ATOM 2030 C LEU B 40 20.341 64.569 67.361 1.00 38.69
6
ATOM 2031 0 LEU B 40 19.853 64.629 68.481 1.00 38.27
8
ATOM 2032 N ARG B 41 21.369 65.321 66.971 1.00 55.38
7
ATOM 2033 CA ARG B 41 22.003 66.298 67.858 1.00 56.87
6
ATOM 2034 CB ARG B 41 23.079 67.091 67.089 1.00 81.26
6
ATOM 2035 CG ARG B 41 23.262 68.555 67.505 1.00 84.64
6
ATOM 2036 CD ARG B 41 22.236 69.446 66.809 1.00 87.81
6
ATOM 2037 NE ARG B 41 22.300 70.844 67.226 1.00 91.69
7
ATOM 2038 CZ ARG B 41 21.468 71.790 66.791 1.00 94.10
6
ATOM 2039 NH1 ARG B 41 20.507 71.488 65.927 1.00 95.61
7
ATOM 2040 NH2 ARG B 41 21.591 73.040 67.220 1.00 95.48
7
ATOM 2041 C ARG B 41 20.939 67.238 68.411 1.00 55.92
6
ATOM 2042 0 ARG B 41 20.902 67.543 69.608 1.00 56.14
8
ATOM 2043 N ARG B 42 20.069 67.692 67.523 1.00 57.87
7
ATOM 2044 CA ARG B 42 19.005 68.579 67.920 1.00 57.13
6
ATOM 2045 CB ARG B 42 18.113 68.868 66.727 1.00 64.73
6
ATOM 2046 CG ARG B 42 17.240 70.072 66.892 1.00 65.29
6
ATOM 2047 CD ARG B 42 16.208 70.092 65.800 1.00 66.30
6
ATOM 2048 NE ARG B 42 15.518 71.364 65.771 1.00 68.31
7
ATOM 2049 CZ ARG B 42 16.147 72.517 65.607 1.00 70.27
6
ATOM 2050 NH1 ARG B 42 17.468 72.527 65.461 1.00 70.63
7
ATOM 2051 NH2 ARG B 42 15.462 73.651 65.588 1.00 71.14
7
ATOM 2052 C ARG B 42 18.229 67.833 68.994 1.00 56.62
6
ATOM 2053 0 ARG B 42 18.336 68.155 70.175 1.00 57.25
8
ATOM 2054 N ILE B 43 17.476 66.817 68.576 1.00 77.76
7
ATOM 2055 CA ILE B 43 16.676 65.998 69.483 1.00 76.69
6
ATOM 2056 CB ILE B 43 16.165 64.741 68.776 1.00 64.03
6
ATOM 2057 CG2 ILE B 43 15.567 63.786 69.783 1.00 62.92
6
ATOM 2058 CG1 ILE B 43 15.147 65.138 67.707 1.00 65.81
6
ATOM 2059 CD1 ILE B 43 14.445 63.971 67.030 1.00 70.53
6
ATOM 2060 C ILE B 43 17.442 65.561 70.721 1.00 76.34
6
ATOM 2061 0 ILE B 43 16.889 65.539 71.818 1.00 76.73
8
ATOM 2062 N LEU B 44 18.709 65.202 70.527 1.00 46.89
7
ATOM 2063 CA LEU B 44 19.601 64.771 71.604 1.00 46.25
6
37

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2064 CB LEU B 44 21.035 64.674 71.090 1.00 38.30
ATOM 2065 CG LEU B 44 21.711 63.320 70.966 1.00 39.26
ATOM 2066 CD1 LEU B 44 20.927 62.307 71.762 1.00 39.28
ATOM 2067 CD2 LEU B 44 21.826 62.931 69.505 1.00 39.06
ATOM 2068 C LEU B 44 19.593 65.742 72.778 1.00 .46.18
ATOM 2069 O LEU B 44 19.448 65.327 73.932 1.00 45.77
ATOM 2070 N LEU B 45 19.768 67.030 72.467 1.00 43.02
ATOM 2071 CA LEU B 45 19.802 68.093 73.471 1.00 42.79
ATOM 2072 CB LEU B 45 20.227 69.412 72.835 1.00 59.53
ATOM 2073 CG LEU B 45 21.718 69.572 72.551 1.00 59.66
ATOM 2074 CD1 LEU B 45 21.947 70.993 72.028 1.00 59.91
ATOM 2075 CD2 LEU B 45 22.548 69.307 73.827 1.00 59.49
ATOM 2076 C LEU B 45 18.485 68.302 74.182 1.00 43.51
ATOM 2077 O LEU B 45 18.447 68.848 75.283 1.00 42.99
ATOM 2078 N SER B 46 17.410 67.867 73.539 1.00139.02
ATOM 2079 CA SER B 46 16.076 68.000 74.095 1.00140.04
ATOM 2080 CB SER B 46 15.203 68.840 73.160 1.00 52.11
ATOM 2081 OG SER B 46 15.746 70.140 73.005 1.00 52.48
ATOM 2082 C SER B 46 15.445 66.631 74.284 1.00141.18
ATOM 2083 O SER B 46 16.113 65.664 74.662 1.00141.89
ATOM 2084 N SER B 47 14.146 66.576 74.022 1.00 62.93
ATOM 2085 CA SER B 47 13.361 65.360 74.121 1.00 62.15
ATOM 2086 CB SER B 47 12.688 65.132 72.773 1.00 33.15
ATOM 2087 OG SER B 47 12.207 66.370 72.280 1.00 34.70
ATOM 2088 C SER B 47 14.147 64.116 74.558 1.00 61.77
ATOM 2089 O SER B 47 14.852 63.499 73.768 1.00 61.78
ATOM 2090 N ILE B 48 14.020 63.780 75.835 1.00 37.83
ATOM 2091 CA ILE B 48 14.660 62.615 76.439 1.00 37.39
ATOM 2092 CB ILE B 48 16.191 62.619 76.259 1.00 16.95
ATOM 2093 CG2 ILE B 48 16.860 61.854 77.420 1.00 17.05
ATOM 2094 CG1 ILE B 48 16.564 61.993 74.915 1.00 14.85
ATOM 2095 CD1 ILE B 48 18.001 61.528 74.848 1.00 15.56
ATOM 2096 C ILE B 48 14.346 62.631 77.930 1.00 39.08
ATOM 2097 O ILE B 48 14.724 63.554 78.638 1.00 39.18
ATOM 2098 N PRO B 49 13.663 61.595 78.430 1.00 39.29
ATOM 2099 CD PRO B 49 13.423 60.297 77.780 1.00 99.40
ATOM 2100 CA PRO B 49 13.312 61.537 79.855 1.00 40.69
ATOM 2101 CB PRO B 49 12.806 60.104 80.025 1.00 99.95
ATOM 2102 CG PRO B 49 13.533 59.355 78.940 1.00100.22
ATOM 2103 C PRO B 49 14.463 61.872 80.803 1.00 41.75
ATOM 2104 O PRO B 49 15.623 61.592 80.490 1.00 41.14
ATOM 2105 N GLY B 50 14.132 62.464 81.956 1.00 70.15
ATOM 2106 CA GLY B 50 15.150 62.840 82.929 1.00 71.83
ATOM 2107 C GLY B 50 14.865 62.678 84.422 1.00 74.05
ATOM 2108 O GLY B 50 14.013 61.892 84.842 1.00 74.59
ATOM 2109 N THR B 51 15.615 63.435 85.222 1.00119.01
ATOM 2110 CA THR B 51 15.527 63.441 86.688 1.00118.26
ATOM 2111 CB THR B 51 16.247 62.277 87.314 1.00 46.81
ATOM 2112 OG1 THR B 51 16.614 62.481 88.640 1.00 46.78
ATOM 2113 CG2 THR B 51 17.319 61.690 86.468 1.00 46.66
ATOM 2114 C THR B 51 16.334 64.623 87.209 1.00119.45
ATOM 2115 0 THR B 51 17.516 64.475 87.534 1.00119.94
ATOM 2116 N ALA B 52 15.706 65.790 87.303 1.00103.75
ATOM 2117 CA ALA B 52 16.428 66.967 87.764 1.00102.87
ATOM 2118 CB ALA B 52 16.697 67.892 86.582 1.00152.39
ATOM 2119 C ALA B 52 15.774 67.756 88.889 1.00102.49
38

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2120 0 ALA B 52 14.593 68.104 88.815 1.00102.50
ATOM 2121 N VAL B 53 16.556 68.038 89.930 1.00 62.89
ATOM 2122 CA VAL B 53 16.071 68.827 91.054 1.00 61.42
ATOM 2123 CB VAL B 53 17.251 69.332 91.935 1.00 38.24
ATOM 2124 CG1 VAL B 53 16.826 70.536 92.761 1.00 37.03
ATOM 2125 CG2 VAL B 53 17.734 68.211 92.852 1.00 37.95
ATOM 2126 C VAL B 53 15.378 70.011 90.404 1.00 62.14
ATOM 2127 0 VAL B 53 15.778 70.444 89.323 1.00 61.57
ATOM 2128 N THR B 54 14.336 70.534 91.031 1.00 59.37
ATOM 2129 CA THR B 54 13.668 71.654 90.412 1.00 61.52
ATOM 2130 CB THR B 54 12.606 71.155 89.423 1.00 86.50
ATOM 2131 OG1 THR B 54 11.751 72.242 89.048 1.00 87.50
ATOM 2132 CG2 THR B 54 11.797 70.029 90.039 1.00 88.32
ATOM 2133 C THR B 54 13.053 72.667 91.362 1.00 62.88
ATOM 2134 0 THR B 54 12.680 73.758 90.935 1.00 62.88
ATOM 2135 N SER B 55 12.968 72.324 92.645 1.00 68.07
ATOM 2136 CA SER B 55 12.390 73.228 93.641 1.00 68.95
ATOM 2137 CB SER B 55 10.867 73.069 93.645 1.00 66.80
ATOM 2138 OG SER B 55 10.243 74.033 94.471 1.00 68.93
ATOM 2139 C SER B 5S 12.980 72.906 95.020 1.00 69.81
ATOM 2140 0 SER B 55 12.898 71.767 95.477 1.00 70.50
ATOM 2141 N VAL B 56 13.567 73.900 95.688 1.00 39.05
ATOM 2142 CA VAL B 56 14.199 73.652 96.985 1.00 39.67
ATOM 2143 CB VAL B 56 15.710 74.006 96.953 1.00 37.61
ATOM 2144 CG1 VAL B 56 16.499 73.068 97.881 1.00 37.82
ATOM 2145 CG2 VAL B 56 16.228 73.935 95.537 1.00 36.61
ATOM 2146 C VAL B 56 13.607 74.376 98.185 1.00 42.16
ATOM 2147 0 VAL B 56 12.975 75.413 98.062 1.00 41.71
ATOM 2148 N TYR B 57 13.835 73.801 99.360 1.00 90.24
ATOM 2149 CA TYR B 57 13.374 74.370 100.621 1.00 91.74
ATOM 2150 CB TYR B 57 12.043 73.739 101.064 1.00 94.82
ATOM 2151 CG TYR B 57 11.673 74.012 102.515 1.00 96.56
ATOM 2152 CD1 TYR B 57 10.822 73.157 103.210 1.00 97.93
ATOM 2153 CE1 TYR B 57 10.521 73.377 104.553 1.00 99.32
ATOM 2154 CD2 TYR B 57 12.204 75.101 103.204 1.00 96.87
ATOM 2155 CE2 TYR B 57 11.911 75.326 104.539 1.00 98.00
ATOM 2156 CZ TYR B 57 11.075 74.463 105.206 1.00 99.30
ATOM 2157 OH TYR B 57 10.812 74.681 106.533 1.00 99.95
ATOM 2158 C TYR B 57 14.439 74.108 101.682 1.00 93.31
ATOM 2159 0 TYR B 57 14.880 72.976 101.870 1.00 93.22
ATOM 2160 N ILE B 58 14.853 75.167 102.364 1.00150.74
ATOM 2161 CA ILE B 58 15.847 75.056 103.418 1.00152.30
ATOM 2162 CB ILE B 58 17.147 75.777 103.039 1.00 89.36
ATOM 2163 CG2 ILE B 58 18.313 75.163 103.785 1.00 89.02
ATOM 2164 CG1 ILE B 58 17.395 75.661 101.538 1.00 90.55
ATOM 2165 CD1 ILE B 58 18.567 76.493 101.057 1.00 92.33
ATOM 2166 C ILE B 58 15.264 75.764 104.628 1.00153.96
ATOM 2167 0 ILE B 58 15.192 76.992 204.637 1.00154.94
ATOM 2168 N GLU B 59 14.834 75.011 105.637 1.00 92.95
ATOM 2169 CA GLU B 59 14.267 75.642 106.820 1.00 93.68
ATOM 2170 CB GLU B 59 14.343 74.7.01108.025 1.00208.87
ATOM 2171 CG GLU B 59 14.156 75.393 109.381 1.00208.87
ATOM 2172 CD GLU B 59 12.790 76.045 109.550 1.00208.87
ATOM,2173 OE1 GLU B 59 12.400 76.865 108.690 1.00208.87
ATOM 2174 OE2 GLU B 59 12.107 75.743 110.553 1.00208.87
ATOM 2175 C GLU B 59 15.024 76.932 107.126 1.00 93.98
39

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2176 O GLU B 59 16.247 76.987 106.976 1.00 94.44
8
ATOM 2177 N ASP B 60 14.277 77.964 107.521 1.00148.93
7
ATOM 2178 CA ASP B 60 14.792 79.291 107.882 1.00148.06
6
ATOM 2179 CB ASP B 60 16.301 79.267 108.164 1.00106.25
6
ATOM 2180 CG ASP B 60 16.636 78.550 109.453 1.00106.75
6
ATOM 2181 OD1 ASP B 60 15.907 78.758 110.449 1.00107.45
8
ATOM 2182 OD2 ASP B 60 17.631 77.793 109.476 1.00105.57
8
ATOM 2183 C ASP B 60 14.491 80.426 106.915 1.00148.12
6
ATOM 2184 0 ASP B 60 13.353 80.887 106.839 1.00148.37
8
ATOM 2185 N VAL B 61 15.503 80.873 106.175 1.00 35.04
7
ATOM 2186 CA VAL B 61 15.327 82.001 105.258 1.00 34.64
6
ATOM 2187 CB VAL B 61 16.673 82.667 104.912'1.00 65.03
6
ATOM 2188 CG1 VAL B 61 16.416 83.941 104.101 1.00 64.61
6
ATOM 2189 CG2 VAL B 61 17.452 82.984 106.194 1.00 65.20
6
ATOM 2190 C VAL B 61 14.586 81.755 103.955 1.00 35.20
6
ATOM 2191 0 VAL B 61 14.997 80.944 103.119 1.00 34.12
8
ATOM 2192 N LEU B 62 13.501 82.513 103.807 1.00 82.75
7
ATOM 2193 CA LEU B 62 12.609 82.476 102.656 1.00 84.93
6
ATOM 2194 CB LEU B 62 11.786 83.76$ 102.600 1.00180.46
6
ATOM 2195 CG LEU B 62, 10.976 84.239 103.811 1.00182.79
6
ATOM 2196 CD1 LEU B 62 10.711 85.733 103.688 1.00183.84
6
ATOM 2197 CD2 LEU B 62 9.670 83.473 103.903 1.00183.75
6
ATOM 2198 C LEU B 62 13.343 82.321 101.329 1.00 85.94
6
ATOM 2199 0 LEU B 62 13.969 81.295 101.055 1.00 86.60
8
ATOM 2200 N HIS B 63 13.240 83.375 100.521 1.00 90.72
7
ATOM 2201 CA HIS B 63 13.831 83.481 99.189 1.00 91.10
6
ATOM 2202 CB HIS B 63 13.728 84.933 98.730 1.00157.38
6
ATOM 2203 CG HIS B 63 12.415 85.571 99.067 1.00158.97
6
ATOM 2204 CD2 HIS B 63 12.118 86.675 99.794 1.00159.64
6
ATOM 2205 ND1 HIS B 63 11.207 85.053 98.650 1.00160.02
7
ATOM 2206 CE1 HIS B 63 10.224 85.810 99.104 1.00160.35
6
ATOM 2207 NE2 HIS B 63 10.750 86.801 99.802 1.00160.12
7
ATOM 2208 C HIS B 63 15.270 82.984 99.043 1.00 91.43
6
ATOM 2209 0 HIS B 63 15.876 82.494 99.995 1.00 91.89
8
ATOM 2210 N GLU B 64 15.811 83.128 97.837 1.00 84.24
7
ATOM 2211 CA GLU B 64 17.157 82.657 97.525 1.00 83.49
6
ATOM 2212 CB GLU B 64 17.241 82.319 96.036 1.00208.87
6
ATOM 2213 CG GLU B 64 18.573 81.744 95.598 1.00208.87
6
ATOM 2214 CD GLU B 64 18.638 81.523 94.101 1.00208.87
6
ATOM 2215 OE1 GLU B 64 18.580 82.520 93.349 1.00208.87
8
ATOM 2216 OE2 GLU B 64 18.743 80.353 93.675 1.00208.87
8
ATOM 2217 C GLU B 64 18.304 83.597 97.885 1.00 82.97
6
ATOM 2218 0 GLU B 64 19.358 83.150 98.336 1.00 83.53
8
ATOM 2219 N PHE B 65 18.105 84.891 97.665 1.00 95.11
7
ATOM 2220 CA PHE B 65 19.132 85.886 97.957 1.00 92.98
6
ATOM 2221 CB PHE B 65 18.695 87.244 97.399 1.00165.17
6
ATOM 2222 CG PHE B 65 17.226 87.531 97.573 1.00168.10
6
ATOM 2223 CD1 PHE B 65 16.702 87.847 98.824 1.00168.98
6
ATOM 2224 CD2 PHE B 65 16.365 87.485 96.480 1.00169.74
6
ATOM 2225 CE1 PHE B 65 15.343 88.112 98.982 1.00170.31
6
ATOM 2226 CE2 PHE B 65 15.004 87.748 96.627 1.00170.48
6
ATOM 2227 CZ PHE B 65 14.493 88.063 97.880 1.00170.73
6
ATOM 2228 C PHE B 65 19.436 85.991 99.452 1.00 91.95
6
ATOM 2229 0 PHE B 65 19.001 86.928 100.122 1.00 91.00
8
ATOM 2230 N SER B 66 20.197 85.027 99.967 1.00 48.43
7
ATOM 2231 CA SER B 66 20.549 85.003 101.381 1.00 46.27
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2232 CB SER B 66 19.443 84.347 102.204 1.00 57.99
6
ATOM 2233 OG SER B 66 19.889 84.077 103.529 1.00 58.99
8
ATOM 2234 C SER B 66 21.840 84.288 101.707 1.00 45.72
6
ATOM 2235 0 SER B 66 22.649 83.984 100.838 1.00 45.98
8
ATOM 2236 N THR B 67 21.996 84.016 102.993 1.00 94.64
7
ATOM 2237 CA THR B 67 23.150 83.345 103.537 1.00 94.16
6
ATOM 2238 CB THR B 67 24.293 84.344 103.771 1.00 57.09
6
ATOM 2239 OG1 THR B 67 24.952 84.609 102.528 1.00 58.32
8
ATOM 2240 CG2 THR B 67 25.284 83.809 104.790 1.00 56.21
6
ATOM 2241 C THR B 67 22.701 82.780 104.870 1.00 95.18
6
ATOM 2242 0 THR B 67 22.184 83.517 105.708 1.00 95.70
8
ATOM 2243 N ILE B 68 22.875 81.475 105.056 1.00 83.60
7
ATOM 2244 CA ILE B 68 22.499 80.829 106.308 1.00 82.48
6
ATOM 2245 CB ILE B 68 21.843 79.447 106.069 1.00114.37
6
ATOM 2246 CG2 ILE B 68 21.663 78.724 107.390 1.00114.07
6
ATOM 2247 CG1 ILE B 68 20.469 79.618 105.413 1.00115.44
6
ATOM 2248 CD1 ILE B 68 20.500 80.251 104.039 1.00117.44
6
ATOM 2249 C ILE B 68 23.764 80.651 107.135 1.00 82.86
6
ATOM 2250 0 ILE B 68 24.690 79.955 106.716 1.00 82.59
8
ATOM 2251 N PRO B 69 23.824 81.301 108.312 1.00 80.58
7
ATOM 2252 CD PRO B 69 22.772 82.186 108.846 1.00122.52
6
ATOM 2253 CA PRO B 69 24.964 81.245 109.234 1.00 79.17
6
ATOM 2254 CB PRO B 69 24.443 81.980 110.465 1.00122.46
6
ATOM 2255 CG PRO B 69 23.526 82.995 109.875 1.00122.69
6
ATOM 2256 C PRO B -69 25.420 79.822 109.557 1.00 79.52
6
ATOM 2257 0 PRO B 69 24.619 78.884 109.563 1.00 79.86
8
ATOM 2258 N GLY B 70 26.713 79.670 109.830 1.00 87.91
7
ATOM 2259 CA GLY B 70 27.250 78.359 110.143 1.00 86.45
6
ATOM 2260 C GLY B 70 27.129 77.449 108.943 1.00 86.68
6
ATOM 2261 0 GLY B 70 26..694 76.305 109.069 1.00 86.14
8
ATOM 2262 N VAL B 71 27.505 77.985 107.780 1.00139.89
7
ATOM 2263 CA VAL B 71 27.471 77.287 106.491 1.00139.35
6
ATOM 2264 CB VAL B 71 26.024 77.202 105.922 1.00 72.29
6
ATOM 2265 CG1 VAL B 71 26.024 76.399 104.645 1.00 73.35
6
ATOM 2266 CG2 VAL B 71 25.089 76.552 106.922 1.00 71.63
6
ATOM 2267 C VAL B 71 28.350 78.065 105.491 1.00139.62
6
ATOM 2268 0 VAL B 71 27.965 79.138 105.023 1.00139.95
8
ATOM 2269 N LYS B 72 29.518 77.510 105.167 1.00 95.33
7
ATOM 2270 CA LYS B 72 30.485 78.134 104.259 1.00 94.09
6
ATOM 2271 CB LYS B 72 31.643 77.172 103.957 1.00 76.78
6
ATOM 2272 CG LYS B 72 32.731 77.814 103.081 1.00 77.29
6
ATOM 2273 CD LYS B 72 33.940 76.909 102.803 1.00 77.12
6
ATOM 2274 CE LYS B 72 34.947 77.591 101.853 1.00 75.39
6
ATOM 2275 NZ LYS B 72 36.160 76.765 101.616 1.00 73.12
7
ATOM 2276 C LYS B 72 29.966 78.685 102.934 1.00 93.33
6
ATOM 2277 0 LYS B 72 30.579 79.586 102.358 1.00 93.92
8
ATOM 2278 N GLU B 73 28.858 78.151 102.437 1.00 48.05
7
ATOM 2279 CA GLU B 73 28.303 78.619 101.171 1.00 46.06
6
ATOM 2280 CB GLU B 73 27.736 77.444 100.379'1.00 61.85
6
ATOM 2281 CG GLU B 73 28.775 76.626 99.653 1.00 62.12
6
ATOM 2282 CD GLU B 73 29.762 75.946 100.576 1.00 61.73
6
ATOM 2283 OE1 GLU B 73 30.707 75.331 100.051 1.00 60.25
8
ATOM 2284 OE2 GLU B 73 29.602 76.016 101.812 1.00 62.43
8
ATOM 2285 C GLU B 73 27.215 79.662 101.360 1.00 45.75
6
ATOM 2286 0 GLU B 73 27.114 80.294 102.413 1.00 46.03
8
ATOM 2287 N ASP B 74 26.413 79.843 100.317 1.00 48.39
7
41

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2288 CA ASP B 74 25.303 80.783 100.336 1.00 48.20
ATOM 2289 CB ASP B 74 25.754 82.199 99.943 1.00100.71
ATOM 2290 CG ASP B 74- 26.104 82.330 98.459 1.00103.97
ATOM 2291 OD1 ASP B 74 27.091 81.708 98.015 1.00106.12
ATOM 2292 ' ASP B 74 25.393 83.062 97.734 1.00104.82
OD2
ATOM 2293 C ASP B 74 24.294 80.277 99.333 1.00 47.37
ATOM 2294 O ASP B 74 24.651 79.984 98.199 1.00 47.73
ATOM 2295 N VAL B 75 23.042 80.169 99.760 1.00 64.39
ATOM 2296 CA VAL B 75 21.953 79.695 98.909 1.00 63.43
ATOM 2297 CB VAL B 75 20.794 80.729 98.850 1.00 47.93
ATOM 2298 CG1 VAL B 75 19.467 80.017 98.584 1.00 47.57
ATOM 2299 CG2 VAL B 75 20.724 81.509 100.145 1.00 48.16
ATOM 2300 C VAL B 75 22.367 79.352 97.472 1.00 63.96
ATOM 2301 0 VAL B 75 22.440 78.179 97.115 1.00 64.80
ATOM 2302 N VAL B 76 22.650 80.362 96.653 1.00 56.51
ATOM 2303 CA VAL B 76 23.028 80.120 95.257 1.00 56.80
ATOM 2304 CB VAL B 76 23.573 81.398 94.585 1.00 61.33
ATOM 2305 CG1 VAL B 76 23.925 81.118 93.128 1.00 62.03
ATOM 2306 CG2 VAL B 76 22.537 82.490 94.658 1.00 60.79
ATOM 2307 C VAL'B 76 24.036 78.993 95.028 1.00 57.25
ATOM 2308 0 VAL B 76 23.783 78.110 94.212 1.00 58.30
ATOM 2309 N GLU B 77 25.171 79.027 95.729 1.00 35.97
ATOM 2310 CA GLU .B77 26.194 77.995 95.582 1.00 35.90
ATOM 2311 CB GLU B 77 27.321 78.183 96.582 1.00 69.87
ATOM 2312 CG GLU B 77 28.508 77.299 96.281 1.00 73.10
ATOM 2313 CD GLU B 77 29.192 77.696 94.990 1.00 76.53
ATOM 2314 OE1 GLU B 77 28.482 77.915 93.986 1.00 78.26
ATOM 2315 OE2 GLU B 77 30.439 77.788 94.972 1.00 78.38
ATOM 2316 C GLU B 77 25.564 76.640 95.805 1.00 36.73
ATOM 2317 0 GLU B 77 25.716 75.749 94.982 1.00 38.73
ATOM 2318 N ILE B 78 24.863 76.479 96.926 1.00 55.17
ATOM 2319 CA ILE B 78 24.170 75.224 97.211 1.00 53.98
ATOM 2320 CB ILE B 78 23.296 75.295 98.500 1.00 56.27
ATOM 2321 CG2 ILE B 78 22.354 74.123 98.541 1.00 57.67
ATOM 2322 CG1 ILE B 78 24.160 75.269 99.767 1.00 56.37
ATOM 2323 CD1 ILE B 78 24.404 76.621 100.397 1.00 56.85
ATOM 2324 C ILE B 78 23.237 75.032 96.024 1.00 53.97
ATOM 2325 0 ILE B 78 23.360 74.061 95.281 1.00 54.16
ATOM 2326 N ILE B 79 22.319 75.987 95.857 1.00 65.75
ATOM 2327 CA ILE B 79 21.336 75.997 94.768 1.00 65.99
ATOM 2328 CB ILE B 79 20.848 77.444 94.451 1.00 69.77
ATOM 2329 CG2 ILE B 79 20.368 77.526 93.008 1.00 69.39
ATOM 2330 CG1 ILE B 79 19.730 77.872 95.415 1.00 70.43
ATOM 2331 CD1 ILE B 79 18.324 77.478 94.988 1.00 73.67
ATOM 2332 C ILE B 79 21.931 75.424 93.496 1.00 67.00
ATOM 2333 0 ILE B 79 21.353 74.534 92.878 1.00 67.69
ATOM 2334 N LEU B .80 23.086 75.956 93.108 1.00 70.57
ATOM 2335 CA LEU B 80 23.781 75.513 91.907 1.00 69.02
ATOM 2336 CB LEU B 80 24.942 76.467 91.597 1.00 26.59
ATOM 2337 CG LEU B 80 24.585 77.959 91.502 1.00 24.04
ATOM 2338 CD1 LEU B 80 25.744 78.700 90.914 1.00 25.12
ATOM 2339 CD2 LEU B 80 23.360 78.177 90.643 1.00 21.84
ATOM 2340 C LEU B 80 24.270 74.073 92.079 1.00 69.55
ATOM 2341 0 LEU B 80 24.059 73.241 91.203 1.00 70.67
ATOM 2342 N ASN B 81 24.917 73.777 93.202 1.00 32.83
ATOM 2343 CA ASN B 81 25.371 72.416 93.465 1.00 32.74
42

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2344 CB ASN B 81 25.758 72.241 94.927 1.00 53.32
6
ATOM 2345 CG ASN B 81 27.021 72.955 95.280 1.00 55.49
6
ATOM 2346 OD1 ASN B 81 28.101 72.609 94.805 1.00 55.56
8
ATOM 2347 ND2 ASN B 81 26.902 73.964 96.123 1.00 57.98
7
ATOM 2348 C ASN B 81 24.210 71.474 93.185 1.00 32.80
6
ATOM 2349 O ASN B 81 24.279 70.598 92.322 1.00 32.36
8
ATOM 2350 N LEU B 82 23.142 71.656 93.943 1.00 63.41
7
ATOM 2351 CA LEU B 82 21.959 70.843 93.784 1.00 63.31
6
ATOM 2352 CB LEU B 82 20.752 71.580 94.373 1.00 53.67
6
ATOM 2353 CG LEU B 82 19.575 70.783 94.940 1.00 53.30
6
ATOM 2354 CD1 LEU B 82 20.075 69.750 95.942 1.00 54.27
6
ATOM 355 'CD2LEU B 82 18.611 71.744 95.612 1.00 52.12
6
ATOM 2356 C LEU B 82 21.759 70.585 92.293 1.00 63.65
6
ATOM 2357 O LEU B 82 21.578 69.447 91.883 1.00 63.99
8
ATOM 2358 N LYS B 83 21.827 71.642 91.484 1.00 50.46
7
ATOM 2359 CA LYS B 83 21.639 71.525 90.036 1.00 50.43
6
ATOM 2360 CB LYS B 83 21.912 72.862 89.345 1.00 61.05
6
ATOM 2361. CG LYS B 83 20.787 73.880 89.423 1.00 61.79
6
ATOM 2362 CD LYS B 83 21.137 75.141 88.614 1.00 63.49
6
ATOM 2363 CE LYS B 83 21.394 74.820 87.123 1.00 65.14
6
ATOM 2364 NZ LYS B 83 21.635 76.001 86.224 1.00 64.59
7
ATOM 2365 C LYS B 83 22.508 70.445 89.394 1.00 50.69
6
ATOM 2366 0 LYS B 83 22.093 69.813 88.426 1.00 50.40
8
ATOM 2367 N GLU B 84 23.710 70.238 89.922 1.00 72.59
7
ATOM 2368 CA GLU B 84 24.602 69.220 89.384 1.00 73.33
6
ATOM 2369 CB GLU B 84 26.060 69.599 89.630 1.00 68.79
6
ATOM 2370 CG GLU B 84 26.556 70.775 88.821 1.00 68.46
6
ATOM 2371 CD GLU B 84 28.062 70.971 88.946 1.00 67.59
6
ATOM 2372 OE1 GLU B 84 28.553 71.199 90.078 1.00 66.17
8
ATOM 2373 OE2 GLU B 84 28.754 70.894 87.904 1.00 66.71
8
ATOM 2374 C GLU B 84 24.336 67.853 90.009 1.00 74.24
6
ATOM 2375 O GLU B 84 25.118 66.920 89.839 1.00 75.23
8
ATOM 2376 N LEU B 85 23.236 67.744 90.744 1.00 56.01
7
ATOM 2377 CA LEU B 85 22.853 66.495 91.398 1.00 55.89
6
ATOM 2378 CB LEU B 85 21.736 66.768 92.400 1.00 82.41
6
ATOM 2379 CG LEU B 85 21.185 65.612 93.226 1.00 83.62
6
ATOM 2380 CD1 LEU B 85 22.252 65.113 94.193 1.00 84.58
6
ATOM 2381 CD2 LEU B 85 19.956 66.085 93.988 1.00 83.69
6
ATOM 2382 C LEU B 85 22.353 65.519 90.346 1.00 56.47
6
ATOM 2383 0 LEU B 85 21.671 65.932 89.412 1.00 56.46
8
ATOM 2384 N VAL B 86 22.686 64.235 90.482 1.00 58.30
7
ATOM 2385 CA VAL B 86 22.227 63.229 89.516 1.00 58.92
6
ATOM 2386 CB VAL B 86 23.416 62.561 88.752 1.00 28.71
6
ATOM 2387 CG1 VAL B 86 22.940 62.023 87.403 1.00 28.70
6
ATOM 2388 CG2 VAL B 86 24.543 63.547 88.545 1.00 26.79
6
ATOM 2389 C VAL B 86 21.422 62.141 90.237 1.00 60.35
6
ATOM 2390 0 VAL B 86 21.984 61.253 90.878 1.00 59.96
8
ATOM 2391 N VAL B 87 20.101 62.229 90.135 1.00 51.71
7
ATOM 2392 CA VAL B 87 19.212 61.267 90.775 1.00 53.76
6
ATOM 2393 CB VAL B 87 17.954 61.954 91.369 1.00 59.83
6
ATOM 2394 CG1 VAL B 87 17.010 60.920 91.937 1.00 60.81
6
ATOM 2395 CG2 VAL B 87 18.352 62.914 92.461 1.00 60.06
6
ATOM 2396 C VAL B 87 18.753 60.286 89.722 1.00 56.80
6
ATOM 2397 O VAL B 87 18.842 60.573 88.532 1..0056.99
8
ATOM 2398 N ARG B 88 18.263 59.133 90.167 1.00109.23
7
ATOM 2399 CA ARG B 88 17.756 58.084 89.282 1.00111.52
6
43

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2400 CB ARG B 88 18.820 57.022 89.069 1.00100.13
6
ATOM 2401 CG ARG B 88 18.348 55.818 88.316 1.00101.90
6
ATOM 2402 CD ARG B 88 19.463 54.818 88.303 1.00103.39
6
ATOM 2403 NE ARG B 88 19.096 53.583 87.638 1.00105.95
7
ATOM 2404 CZ ARG B 88 19.944 52.583 87.443 1.00107.64
6
ATOM 2405 NH1 ARG B 88 21.198 52.691 87.869 1.00108.21
7
ATOM 2406 NH2 ARG B 88 19.543 51.483 86.820 1.00108.48
7
ATOM 2407 C ARG B 88 16.541 57.465 89.958 1.00113.37
6
ATOM 2408 0 ARG B 88 16.484 57.404 91.186 1.00113.54
8
ATOM 2409 N PHE B 89 15.578 56.985 89.177 1.00115.12
7
ATOM 2410 CA PHE B 89 24.381 56.437 89.796 1.00116.99
6
ATOM 2411 CB PHE B 89 13.196 57.380 89.500 1.00 73.14
6
ATOM 2412 CG PHE B 89 12.686 57.336 88.074 1.00 73.17
6
ATOM 2413 CD1 PHE B 89 11.592 58.120 87.704 1.00 73.82
6
ATOM 2414 CD2 PHE B 89 13.253 56.495 87.120 1.00 73.06
6
ATOM 241 CE1 PHE B 89 11.064 58.069 86.418 1.00 73.82
6
ATOM 2416 CE2 PHE B 89 12.735 56.432 85.827 1.00 73.37
6
ATOM 2417 CZ PHE B 89 11.635 57.222 85.476 1.00 73.73
6
ATOM 2418 C PHE B 89 14.001 54.976 89.506 1.00119.15
6
ATOM 2419 0 PHE B 89 12.822 54.619 89.549 1.00119.44
8
ATOM 2420 N LEU B 90 14.995 54.128 89.249 1.00130.10
7
ATOM 2421 CA LEU B 90 14.734 52.720 88.949 1.00131.31
6
ATOM 2422 CB LEU B 90 13.990 52.058 90.108 2.00161.35
6
ATOM 2423 CG LEU B 90 14.742 52.079 91.438 1.00162.04
6
ATOM 2424 CD1 LEU B 90 13.912 51.378 92.491 1.00162.42
6
ATOM 2425 CD2 LEU B 90 16.090 51.392 91.286 1.00162.37
6
ATOM 2426 C LEU B 90 13.906 52.648 87.671 1.00132.86
6
ATOM 2427 0 LEU B 90 14.464 52.494 86.584 1.00132.98
8
ATOM 2428 N ASP B 91 12.582 52.752 87.803 1.00128.55
7
ATOM 2429 CA ASP B 91 11.689 52.763 86.638 1.00128.88
6
ATOM 2430 ,CB ASP B 91 12.144 51.738 85.594 1.00154.78
6
ATOM 2431 CG ASP B 91 11.860 52.197 84.172 1.00155.70
6
ATOM 2432 OD1 ASP B 91 10.676 52.416 83.835 1.00155.84
8
ATOM 2433 OD2 ASP B 91 12.824 52.355 83.390 1.00156.27
8
ATOM 2434 C ASP B 91 10.191 52.576 86.878 1.00129.05
6
ATOM 2435 O ASP B 91 9.505 51.976 86.055 1.00129.10
8
ATOM 2436 N PRO B 92 9.659 53.079 88.004 1.00 52.86
7
ATOM 2437 CD PRO B 92 10.297 53.428 89.286 1.00163.39
6
ATOM 2438 CA PRO B 92 8.217 52.880 88.180 1.00 52.64
6
ATOM 2439 CB PRO B 92 8.073 52.807 89.694 1.00163.65
6
ATOM 2440 CG PRO B 92 9.110 53.772 90.155 1.00163.70
6
ATOM 2441 C PRO B 92 7.411 54.028 87.566 1.00 52.65
6
ATOM 2442 0 PRO B 92 7.342 54.172 86.345 1.00 52.29
8
ATOM 2443 N ARG B 93 6.794 54.824 88.433 1.00159.93
7
ATOM 2444 CA ARG B 93 6.006 55.993 88.051 1.00160.97
6
ATOM 2445 CB ARG B 93 4.510 55.665 88.086 1.00102.93
6
ATOM 2446 CG ARG B 93 3..589 56.842 87.749 1.00103.89
6
ATOM 2447 CD ARG B 93 2.214 56.512 88.042 1.00104.42
6
ATOM 2448 NE ARG B 93 1.273 57.709 88.143 1.00103.76
7
ATOM 2449 CZ ARG B 93 0.003 57.706 88:545 1.00103.70
6
ATOM 2450 NH1 ARG B 93 -0.586 56.568 88.885 1.00103.43
7
ATOM 2451 NH2 ARG B 93 -0.678 58.842 88.616 1.00104.03
7
ATOM 2452 C ARG B 93 6.380 56.981 89.155 1.00160.96
6
ATOM 2453 0 ARG B 93 5.556 57.381 89.974 1.00161.51
8
ATOM 2454 N TRP B 94 7.657 57.349 89.151 1.00171.63
7
ATOM 2455 CA TRP B 94 8.276 58.230 90.139'1.00170.47
6
44

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2456 CB TRPB 94 9.763 57.888 90.163 1.00167.50
6
ATOM 2457 CG TRPB 94 10.568 58.559 91.201 1.00170.85
6
ATOM 2458 CD2 TRPB 94 11.423 59.692 91.019 1.00171.98
6
ATOM 2459 CE2 TRPB 94 12.024 59.963 92.262 1.00173.23
6
ATOM 2460 CE3 TRPB 94 11.740 60.505 89.922 1.00171.41
6
ATOM 2461 CD1 TRPB 94 10.676 58.200 92.507 1.00172.08
6
ATOM 2462 NE1 TRPB 94 11.550 59.036 93.153 1.00173.59
7
ATOM 2463 CZ2 TRPB 94 12.927 61.013 92.446 1.00174.27
6
ATOM 2464 CZ3 TRPB 94 12.637 61.549 90.103 1.00172.06
6
ATOM 2465 CH2 TRPB 94 13.220 61.792 91.356 1.00173.80
6
ATOM 2466 C TRPB 94 8.086 59.748 89.972 1.00168.43
6
ATOM 2467 O TRPB 94 7.625 60.219 88.929 1.00168.12
8
ATOM 2468 N ARGB 95 8.458 60.489 91.022 1.00108.70
7
ATOM 2469 CA ARGB 95 8.374 61.957 91.100 1.00105.59
6
ATOM 2470 CB ARGB 95 7.074 62.460 90.462 1.00152.49
6
ATOM 2471 CG ARGB 95 5.833 61.748 90.970 1.00153.87
6
ATOM 2472 CD ARGB 95 4.759 62.732 91.395 1.00154.72
6
ATOM 2473 NE ARGB 95 3.626 62.056 92.019 1.00156.37
7
ATOM 2474 CZ ARGB 95 2.569 62.682 92.525 1.00157.56
6
ATOM 2475 NH1 ARGB 95 2.494 64.007 92.483 1.00158.22
7
ATOM 2476 NH2 ARGB 95 1.586 61.983 93.077 1.00158.25
7
ATOM 2477 C ARGB 95 8.405 62.356 92.583 1.00103.14
6
ATOM 2478 O ARGB 95 7.460 62.954 93.096 1.00103.69
~ 8
ATOM 2479 N THRB 96 9.509 62.045 93.258 1.00 76.25
7
ATOM 2480 CA THRB 96 9.641 62.303 94.694 1.00 73.20
6
ATOM 2481 CB THRB 96 10.349 61.171 95.379 1.00 62.79
6
ATOM 2482 OG1 THRB 96 11.738 61.215 95.027 1.00 60.49
8
ATOM 2483 CG2 THRB 96 9.727 59.850 94.975 1.00 64.35
6
ATOM 2484 C THRB 96 10.340 63.545 95.211 1.00 72.96
6
ATOM 2485 0 THRB 96 10.643 64.476 94.470 1.00 73.64
8
ATOM 2486 N THRB 97 10.602 63.512 96.519 1.00 58.24
7
ATOM 2487 CA THRB 97 11.256 64.595 97.253 1.00 54.45
6
ATOM 2488 CB THRB 97 10.249 65.333 98.180 1.00 57.05
6
ATOM 2489 OG1 THRB 97 9.224 65.949 97.388 1.00 57.08
8
ATOM 2490 CG2 THRB 97 10.957 66.393 99.012 1.00 57.27
6
ATOM 2491 C THRB 97 12.391 64.041 98.118 1.00 53.16
6
ATOM 2492 O THRB 97 12.416 62.857 98.457 1.00 51.75
8
ATOM 2493 N LEUB 98 13.321 64.918 98.473 1.00 49.32
7
ATOM 2494 CA LEUB 98 14.466 64.560 99.290 1.00 48.21
6
ATOM 2495 CB LEUB 98 15.746 64.676 98.468 1.00126.78
6
ATOM 2496 CG LEUB 98 15.672 64.157 97.032 1.00128.47
6
ATOM 2497 CD1 LEUB 98 16.965 64.491 96.312 1.00129.98
6
ATOM 2498 CD2 LEUB 98 15.415 62.658 97.030 1.00129.19
6
ATOM 2499 C LEUB 98 14.526 65.543 100.440 1.00 48.83
6
ATOM 2500 0 LEUB 98 14.054 66.669 100.322 1.00 47.56
8
ATOM 2501 N ILEB 99 15.107 65.115 101.551 1.00 81.01
7
ATOM 2502 CA ILEB 99 15.238 65.973 102.718 1.00 82.32
6
ATOM 2503 CB ILEB 99 14.221 65.573 103.785 1.00105.79
6
ATOM 2504 CG2 ILEB 99 14.372 66.445 105.007 1.00105.81
6
ATOM 2505 CG1 ILEB 99 12.813 65.723 103.205 1.00105.76
6
ATOM 2506 CD1 ILEB 99 11.713 65.364 104.160 1.00105.01
6
ATOM 2507 C ILEB 99 16.669 65.842 103.232 1.00 85.52
6
ATOM 2508 O ILEB 99 17.411 64.993 102.749 1.00 86.30
8
ATOM 2509 N LEUB 100 17.076 66.659 104.199 1.00129.42
7
ATOM 2510 CA LEUB 100 18.455 66.571 104.660 1.00130.47
6
ATOM 2511 CB LEUB 100 19.299 67.559 103.854 1.00 82.55
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2512 CG LEU B 100 20.786 67.238 103.7021.00 85.11
6
ATOM 2513 CD1 LEU B 100 20.965 65.792 103.2801.00 85.38
6
ATOM 2514 CD2 LEU B 100 21.403 68.169 102.6751.00 86.72
6
ATOM 2515 C LEU B 100 18.701 66.769 106.1591.00132.38
6
ATOM 2516 0 LEU B 100 17.874 67.342 106.870'1.00132.51
8
ATOM 2517 N ALA B 101 19.856 66.285 106.6191.00 98.92
7
ATOM 2518 CA ALA B 101 20.270 66.373 108.0221.00 99.56
6
ATOM 2519 CB ALA B 101 20.205 64.989 108.6621.00 57.99
6
ATOM 2520 C ALA B 101 21.694 66.933 108.1191.00100.36
6
ATOM 2521 O ALA B 101 22.639 66.340 107.5941.00100.49
8
ATOM 2522 N ALA B 102 21.852 68.064 108.8031.00 90.68
7
ATOM 2523 CA ALA B 102 23.164 68.691 108.9201.00 91.70
6
ATOM 2524 CB ALA B 102 23.083 70.128 108.4241.00114.01
6
ATOM 2525 C ALA B '102 23.796 68.656 110.3121.00 94.60
6
ATOM 2526 0 ALA B 102 23.135 68.904 111.3211.00 95.30
8
ATOM 2527 N ALA B 103 25.095 68.367 110.3421.00107.85
7
ATOM 2528 CA ALA B 103 25.858 68.287 111.5811.00109.82
6
ATOM 2529 CB ALA B 103 26.539 66.929 111.6751.00162.25
6
ATOM 2530 C ALA B 103 26.900 69.401 111.6961.00110.77
6
ATOM 2531 0 ALA B 10~ 26.803 70.426 111.0271.00111.66
8
ATOM 2532 N GLY B 104 27.903 69.183 112.5421.00119.02
7
ATOM 2533 CA GLY B 104 28.945 70.174 112.7561.00117.56
6
ATOM 2534 C GLY B 104 29.728 70.570 111.5221.00118.84
6
ATOM 2535 0 GLY B 104 29.191 70.570 110.4141.00118.81
8
ATOM 2536 N PRO B 105 31.018 70.901 111.6851.00112.08
7
ATOM 2537 CD PRO B 105 31.762 70.769 112.9491.00147.49
6
ATOM 2538 CA PRO B 105 31.913 71.313 110.5961.00111.87
6
ATOM 2539 CB PRO B 105 33.275 71.415 111.2901.00147.97
6
ATOM 2540 CG PRO B 105 33.148 70.476 112.4571.00147.73
6
ATOM 2541 C PRO B 105 31.959 70.448 109.3261.00112.31
6
ATOM 2542 0 PRO B 105 32.227 70.955 108.2381.00112.30
8
ATOM 2543 N LYS B 106 31.690 69.155 109.4661.00131.56
7
ATOM 2544 CA LYS B 106 31.717 68.219 108.3411.00130.72
6
ATOM 2545 CB LYS B 106 31.027 66.908 108.7421.00199.00
6
ATOM 2546 CG LYS B 106 31.550 66.288 110.0371.00199.67
6
ATOM 2547 CD LYS B 106 33.040 65.977 109.9521.00200.50
6
ATOM 2548 CE LYS B 106 33.571 65.438 111.2731.00201.17
6
ATOM 2549 NZ LYS B 106 35.043 65.192 111.2481.00201.26
7
ATOM 2550 C LYS B 106 31.080 68.750 107.0531.00130.11
6
ATOM 2551 O LYS B 106 30.327 69.728 107.0721.00130.08
8
ATOM 2552 N GLU B 107 31.398 68.090 105.9371.00 46.75
7
ATOM 2553 CA GLU B 107 30.868 68.449 104.6251.00 45.13
6
ATOM 2554 CB GLU B 107 31.787 67.913 103.527.1.00152.42
6
ATOM 2555 CG GLU B 107 33.188 68.481 103.5691.00155.40
6
ATOM 2556 CD GLU B 107 34.072 67.928 102.4711.00158.04
6
ATOM 2557 OE1 GLU B 107 33.693 68.031 101.2861.00159.56
8
ATOM 2558 OE2 GLU B 107 35.153 67.393 102.7891.00159.19
8
ATOM 2559 C GLU B 107 29.460 67.877 104.4301.00 44.32
6
ATOM 2560 0 GLU B 107 29.110 66.857 105.0121.00 43.92
8
ATOM 2561 N VAL B 108 28.640 68.544 103.6271.00 79.93
7
ATOM 2562 CA VAL B 108 27.296 68.044 103.3651.00 79.62
6
ATOM 2563 CB VAL B 108 26.192 69.146 103.5271.00'52.82
6
ATOM 2564 CG1 VAL B 108 24.827 68.584 103.1841.00 51.88
6
ATOM 2565 CG2 VAL B 108 26.154 69.646 104.9551.00 54.40
6
ATOM 2566 C VAL B 108 27.309 67.539 101.9331.00 79.72
6
ATOM 2567 0 VAL B 108 26.995 68.269 100.9971.00 81.03
8
46

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2568 N ARGB 109 27.712 66.286 101.773 1.00 72.39 7
ATOM 2569 CA ARGB 109 27.761 65..661100.462 1.00 71.53 6
ATOM 2570 CB ARGB 109 28.515 64.336 100.543 1.00 75.22 6
'
ATOM 2571 CG ARGB 109 29.606 64.308 101.590 1.00 74.93 6
ATOM 2572 CD ARGB 109 30.934 63.925 100.968 1.00 74.31 6
ATOM 2573 NE ARGB 109 32.020 63.961 101.939 1.00 74.49 7
ATOM 2574 CZ ARGB 109 33.268 64.308 101.646 1.00 75.44 6
ATOM 2575 NH1 ARGB 109 33.589 64.654 100.404 1.00 75.90 7
ATOM 2576 NH2 ARGB 109 34.193 64.310 102.595 1.00 76.18 7
ATOM 2577 C ARGB 109 26.331 65.381 100.012 1.00 71.79 6
ATOM 2578 O ARGB 109 25.411 65.321 100:834 1.00 72.18 8
ATOM 2579 N ALAB /110 26.137 65.199 98.712 1.00 60.56 7
ATOM 2580 CA ALAB 110 24.809 64.890 98.204 1.00 60.62 6
ATOM 2581 CB ALAB 110 24.823 64.883 96.680 1.00116.89 6
ATOM 2582 C ALAB 110 24.408 63.511 98.745 1.00 60.99 6
ATOM 2583 O ALAB 110 23.337 62.990 98.436 1.00 60.39 8
ATOM 2584 N VALB 11l 25.287 62.928 99.556 1.00115.68 7
ATOM 2585 CA VALB 11l 25.054 61.617 100.152 1.00116.53 6
ATOM 2586 CB VALB 111 26.381 60.972 100.638 1.00171.54 6
ATOM 2587 CG1 VALB 111 26.136 59.533 101.086 1.00171.02 6
ATOM 2588 CG2 VALB 111 27.422 61.017 99.534 1.00172.39 6
ATOM 2589 C VALB 111 24.117 61.739 101.348 1.00116.52 6
ATOM 2590 0 VALB 111 23.121 61.021 101.446 1.00116.08 8
ATOM 2591 N ASPB 112 24.446 62.653 102.257 1.00 93.32 7
ATOM 2592 CA ASPB 112 23.649 62.875 103.458 1.00 93.72 6
ATOM 2593 CB ASPB 112 23.949 64.258 104.039 1.00208.87 6
ATOM 2594 CG ASPB 112 25.386 64.397 104.498 1.00208.87 6
ATOM 2595 OD1 ASPB 112 25.746 65.487 104.988 1.00208.87 8
ATOM 2596 OD2 ASPB 112 26.154 63.420 104.374 1.00208.87 8
ATOM 2597 C ASPB 112 22.161 62.754 103.174 1.00 93.88 6
ATOM 2598 O ASPB 112 21.403 62.243 104.000 1.00 93.49 8
ATOM 2599 N PHEB 113 21.754 63.223 101.997 1.00140.32 7
ATOM 2600 CA PHEB 113 20.357 63.175 101.587 1.00139.27 6
ATOM 2601 CB PHEB 113 20.239 63.102 100.059 1.00 88.94 6
ATOM 2602 CG PHEB 113 20.398 64.431 99.369 1.00 88.94 6
ATOM 2603 CD1 PHEB 113 21.643 65.049 99.284 1.00 89.09 6
ATOM 2604 CD2 PHEB 113 19.295 65.069 98.804 1.00 88.14 6
ATOM 2605 CE1 PHEB 113 21.792 66.285 98.645 1.00 89.50 6
ATOM 2606 CE2 PHEB 113 19.429 66.305 98.164 1.00 88.22 6
ATOM 2607 CZ PHEB 113 20.685 66.915 98.084 1.00 89.00 6
ATOM 2608 C PHEB 113 19.639 61.984 102.199 1.00139.39 6
ATOM 2609 0 PHEB 113 20.129 60.859 102.125 1.00139.80 8
ATOM 2610 N THRB 114 18.491 62.257 102.819 1.00 89.09 7
ATOM 2611 CA THRB 114 17.644 61.240 103.444 1.00 88.38 6
ATOM 2612 CB THRB 114 16.190 61.743 103.574 1.00160.36 6
ATOM 2613 OG1 THRB 114 16.151 62.882 104.444 1.00160.56 8
ATOM 2614 CG2 THRB 114 15.290 60.644 104.121 1.00160.45 6
ATOM 2615 C THRB 114 17.647 59.997 102.566 1.00 88.16 6
ATOM 2616 0 THRB 114 16.781 59.844 101.699 1.00 87.90 8
ATOM 2617 N PROB 115 18.610 59.086 102.802 1.00 71.69 7
ATOM 2618 CD PROB 115 19.412 59.070 104.038 1.00 97.44 6
ATOM 2619 CA PROB 115 18.791 57.832 102.060 1.00 71.19 6
ATOM 2620 CB PROB 115 19.749 57.035 102.953 1.00 97.50 6
ATOM 2621 CG PROB 115 19.505 57.603 104.325 1.00 97.83 6
ATOM 2622 C PROB 115 17.526 57.059 101.704 1.00 71.53 6
ATOM 2623 O PROB 115 17.328 55.931 102.147 1.00 71.33 8
47

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2624 N SER B 116 16.683 57.677 100.8841.00146.16
7
ATOM 2625 CA SER B 116 15.444 57.059 100.4441.00146.33
6
ATOM 2626 CB SER B 116 14.477 58.129 99.921 1.00 82.24
6
ATOM 2627 OG SER B 116 13.264 57.556 99.459 1.00 80.53
8
ATOM 2628 C SER B 116 15.770 56.058 99.341 1.00147.50
6
ATOM 2629 0 SER B 116 16.562 56.347 98.442 1.00147.96
8
ATOM 2630 N ALA B 117 15.175 54.873 99.423 1.00154.86
7
ATOM 2631 CA ALA B 117 15.407 53.841 98.422 1.00154.61
6
ATOM 2632 CB ALA B 117 14.884 52.505 98.920 1.00152.66
6
ATOM 2633 C ALA B 117 14.695 54.240 97.141 1.00155.24
6
ATOM 2634 0 ALA B 117 14.167 55.347 97.042 1.00155.74
8
ATOM 2635 N ASP B 118 14.688 53.339 96.166 1.00 88.55
7
ATOM 2636 CA ASP B 118 14.037 53.588 94.887 1.00 88.64
6
ATOM 2637 CB ASP B 218 12.510 53.457 95.026 1.00218.28
. 6
ATOM 2638 CG ASP B 118 11.902 54.515 95.930 1.00119.07
6
ATOM 2639 OD1 ASP B 118 11.985 54.381 97.170 1.00119.87
8
ATOM 2640 OD2 ASP B 118 11.341 55.489 95.389 1.00119.11
8
ATOM 264 C ASP B 118 14.385 54.950 94.293 1.00 88.12
1 6
ATOM 2642 0 ASP B 118 13.772 55.391 93.323 1.00 87.96
8
ATOM 2643 N VAL B 119 15.382 55.603 94.882 1.00 77.20
7
ATOM 2644 CA VAL B 119 15.845 56.922 94.460 1.00 77.42
6
ATOM 2645 CB VAL B 119 14.940 58.045 95.043 1.00 51.81
6
ATOM 2646 CG1 VAL B 119 15.413 59.407 94.585 1.00 51.41
6
ATOM 2647 CG2 VAL B 119 13.512 57.829 94.616 1.00 51.98
6
ATOM 2648 C VAL B 119 17.258 57.079 95.018 1.00 77.32
6
ATOM 2649 0 VAL B 119 17.451 57.666 96.085 1.00 77.65
8
ATOM 2650 N GLU B 120 18.244 56.535 94.314 1.00142.78
7
ATOM 2651 CA GLU B 120 19.624 56.634 94.775 1.00142.83
6
ATOM 2652 CB GLU B 120 20.325 55.271 94.669 1.00114.74
6
ATOM 2653 CG GLU B 120 21.680 55.207 95.375 1.00115.85
6
ATOM 2654 CD GLU B 120 22.377 53.868 95.205 1.00117.05
6
ATOM 2655 OE1 GLU B 120 22.751 53.520 94.063 1.00117.57
8
ATOM 2656 OE2 GLU B 120 22.546 53.160 96.217 1.00117.15
8
ATOM 2657 C GLU B 120 20.398 57.684 93.979 1.00143.11
6
ATOM 2658 O GLU B 120 20.442 57.645 92.746 1.00143.41
8
ATOM 2659 N ILE B 121 20.991 58.631 94.699 1.00 89.19
7
ATOM 2660 CA ILE B 121 21.776 59.686 94.083 1.00 87.07
6
ATOM 2661 CB ILE B 121 22.254 60.715 95.143 1.00137.58
6
ATOM 2662 CG2 ILE B 121 23.420 61.516 94.608 1.00138.22
6
ATOM 2663 GG1 ILE B 121 21.112 61.665 95.515 1.00137.41
6
ATOM 2664 CD1 ILE B 121 19.941 61.000 96.191 1.00137.99
6
ATOM 2665 C ILE B 121 22.980 59.075 93.365 1.00 86.78
6
ATOM 2666 0 ILE B 121 23.945 58.627 93.996 1.00 85.86
8
ATOM 2667 N MET B 122 22.883 59.058 92.037 1.00 88.09
7
ATOM 2668 CA MET B 122 23.902 58.529 91.131 1.00 86.88
6
ATOM 2669 CB MET B 122 23.585 58.993 89.709 1.00 98.50
6
ATOM 2670 CG MET B 122 23.708 57.940 88.638 1.00100.12
6
ATOM 2671 SD MET B 122 22.126 57.147 88.335 1.00102.51
16
ATOM 2672 CE MET B 122 22.120 55.932 89.664 1.00103.27
6
ATOM 2673 C MET B 122 25.310 59.009 91.489 1.00 86.12
6
ATOM 2674 O MET B 122 26.288 58.265 91.379 1.00 86.13
8
ATOM 2675 N ASN B 123 25.383 60.273 91.896 1.00102.30
7
ATOM 2676 CA ASN B 123 26.628 60.944 92.259 1.00100.44
6
ATOM 2677 CB ASN B 123 26.719 62.251 91.479 1.00 44.03
6
ATOM 2678 CG ASN B 123 25.395 62.996 91.442 1.00 43.06
6
ATDM 2679 OD1 ASN B 123 24.329 62.407 91.626 1.00 41.66
8
4~

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2680 ND2 ASN B 123 25.457 64.299 91.184 1.00 42.20
7
ATOM 2681 C ASN B 123 26.728 61.212 93.763 1.00100.01
6
ATOM 2682 O ASN B 123 26.158 62.172 94.278 1.00100.97
8
ATOM 2683 N PRO B 124 27.492 60.375 94.479 1.00 40.54
7
ATOM 2684 CD PRO B 124 28.486 59.467 93.880 1.00130.63
6
ATOM 2685 CA PRO B 124 27.699 60.466 95.923 1.00 38.86
6
ATOM 2686 CB PRO B 124 28.775 59.419 96.181 1.00129.78
6
ATOM 2687 CG PRO B 124 29.578 59.461 94.923 1.00130.60
6
ATOM 2688 C PRO B 124 28.124 61.831 96.413 1.00 38.04
6
ATOM 2689 0 PRO B 124 27.353 62.534 97.059 1.00 37.31
8
ATOM 2690 N ASP B 125 29.361 62.191 96.100 1.00 96.40
7
ATOM 2691 CA ASP B 125 29.952 63.453 96.517 1.00 97.07
6
ATOM 2692 CB ASP B 125 31.336 63.556 95.900 1.00 90.27
6
ATOM 2693 CG ASP B 125 32.054 62.228 95.911 1.00 89.52
6
ATOM 2694 OD1 ASP B 125 32.136 61.608 96.992 1.00 88.48
8
ATOM 2695 OD2 ASP B 125 32.526 61.797 94.841 1.00 90.20
8
ATOM 2696 C ASP B 125 29.129 64.698 96.201 1.00 97.77
6
ATOM 2697 O ASP B 125 27.97.6 64.809 96.623 1.00 98.95
8
ATOM 2698 N LEU B 126 29.725 65.640 95.473 1.00 65.34
7
ATOM 2699 CA LEU B 126 29.038 66.882 95.114 1.00 64.52
6
ATOM 2700 CB LEU B 126 27.659 66.584 94.511 1.00106.19
6
ATOM 2701 CG LEU B 126 26.828 67.776 94.031 1.00106.27
6
ATOM 2702 CD1 LEU B 126 27.577 68.543 92.956 1.00106.51
6
ATOM 2703 CD2 LEU B 126 25.506 67.274 93.489 1.00106.74
6
ATOM 2704 C LEU B 126 28.884 67.736 96.363 1.00 64.64
6
ATOM 2705 O LEU B 126 27.928 67.584 97.121 1.00 65.05
8
ATOM 2706 N HIS B 127 29.831 68.641 96.558 1.00 66.42
7
ATOM 2707 CA HIS B 127 29.862 69.514 97.719 1.00 65.85
6
ATOM 2708 CB HIS B 127 30.625 70.790 97.376 1.00101.20
6
ATOM 2709 CG HIS B 127 31.028 71.577 98.579 1.00103.06
6
ATOM 2710 CD2 HIS B 127 32.243 71.799 99.130 1.00104.05
6
ATOM 2711 ND1 HIS B 127 30.111 72.177 99.413 1.00103.81
7
ATOM 2712 CE1 HIS B 127 30.746 72.732 100.429 1.00103.99
6
ATOM 2713 NE2 HIS B 127 32.040 72.517 100.282 1.00104.05
7
ATOM 2714 C HIS B 127 28.545 69.893 98.417 1.00 66.02
6
ATOM 2715 0 HIS B 127 28.417 69.719 99.633 1.00 65.62
8
ATOM 2716 N ILE B 128 27.581 70.421 97.666 1.00 79.55
7
ATOM 2717 CA ILE B 128 26.292 70.848 98.230 1.00 79.18
6
ATOM 2718 CB ILE B 128 25.625 69.750 99.095 1.00 63.78
6
ATOM 2719 CG2 ILE B 128 24.424 70.340 99.820 1.00 62.99
6
ATOM 2720 CG1 ILE B 128 25.170 68.571 98.231 1.00 65.78
6
ATOM 2721 CD1 ILE B 128 23.850 68.808 97.493 1.00 68.57
6
ATOM 2722 C ILE B 128 26.410 72.102 99.104 1.00 79.47
6
ATOM 2723 0 ILE B 128 25.657 73.057 98.916 1.00 80.04
8
ATOM 2724 N ALA B 129 27.339 72.084 100.062 1.00 55.47
7
ATOM 2725 CA ALA B 129 27.575 73.206 100.980 1.00 54.86
6
ATOM 2726 CB ALA B 129 26.259 73.847 101.405 1.00 23.67
6
ATOM 2727 C ALA B 129 28.325 72.717 102.216 1.00 55.53
6
ATOM 2728 O ALA B 129 28.192 71.562 102.614 1.00 56.09
8
ATOM 2729 N THR B 130 29.114 73.607 102.814 1.00 50.17
7
ATOM 2730 CA THR B 130 29.904 73.287 104.000 1.00 50.46
6
ATOM 2731 CB THR B 130 31.407 '73.620103.808 1.00 75.42
6
ATOM 2732 OG1 THR B 130 32.044 72.594 103.034 1.00 75.20
8
ATOM 2733 CG2 THR B 130 32.099 73.726 105.145 1.00 75.41
6
ATOM 2734 C THR B 130 29.390 74.121 105.139 1.00 51.78
6
ATOM 2735 0 THR B 130 29.106 75.296 104.958 1.00 50.09
8
49

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2736 N LEU B 131 29.293 73.503 106.312 1.00126.03
7
ATOM 2737 CA LEU B 131 28.801 74.154 107.525 1.00127.64
6
ATOM 2738 CB LEU B 131 27.337 73.735 107.756 1.00 64.76
6
ATOM 2739 CG LEU B 131 26.947 72.261 107.965 1.00 64.71
6
ATOM 2740 CD1 'LEUB 131 25.510 72.063 107.494 1.00 64.74
6
ATOM 2741 CD2 LEU B 131 27.878 71.331 107.197 1.00 65.16
6
ATOM 2742 C LEU B 131 29.693 73.740 108.704 1.00129.43
6
ATOM 2743 O LEU B 131 30.266 72.653 108.677 1.00128.82
8
ATOM 2744 N GLU B 132 29.822 74.586 109.729 1.00 97.60
7
ATOM 2745 CA GLU B 132 30.684 74.235 110.861 1.00 99.00
6
ATOM 2746 CB GLU B 132 31.951 75.105 110..8281.00134.43
6
ATOM 2747 CG GLU B 132 33.013 74.695 111.847 1.00136.66
6
ATOM 2748 CD GLU B 132 34.383 75.287 111.558 1.00137.83
6
ATOM 2749 OE2 GLU B 132 34.957 74.968 110.494 1.00137.78
8
ATOM 2750 OE2 GLU B 132 34.884 76.069 112.394 1.00138.17
8
ATOM 2751 C GLU B 132 30.079 74.236 112.284 1.00 99.83
6
ATOM 2752 0 GLU B 132 29.306 73.337 112.638 1.00 99.70
8
ATOM 2753 N GLU B 133 30.453 75.226 113.099 1.00 95.71
7
ATOM 2754 CA GLU B 133 29.981 75.354 114.488 1.00 95.88
6
ATOM 2755 CB GLU B 133 30.861 76.365 115.246 1.00 81.48
6
ATOM 2756 CG GLU B 133 30.730 76.360 116.784 1.00 82.68
6
ATOM 2757 CD GLU B 133 31.455 77.541 117.459 1.00 82.94
6
ATOM 2758 OE1 GLU B 133 30.925 78.675 117.431 1.00 83.01
8
ATOM 2759 OE2 GLU B 133 32.561 77.340 118.011 1.00 81.98
8
ATOM 2760 C GLU B 133 28.519 75.797 114.553 1.00 96.07
6
ATOM 2761 0 GLU B 133 28.080 76.390 115.537 1.00 95.61
8
ATOM 2762 N GLY B 134 27.776 75.505 113.491 1.00119.75
7
ATOM 2763 CA GLY B 134 26.371 75.863 113.416 1.00119.61
6
ATOM 2764 C GLY B 134 25.701 74.923 112.436 1.00120.73
6
ATOM 2765 O GLY B 134 25.064 75.351 111.471 1.00120.38
8
ATOM 2766 N GLY B 135 25.865 73.629 112.703 1.00112.87
7
ATOM 2767 CA GLY B 135 25.314 72.589 111.853 1.00112.15
6
ATOM 2768 C GLY B 135 23.805 72.511 111.756 1.00112.82
6
ATOM 2769 O GLY B 135 23.200 71.521 112.175 1.00113.01'
8
ATOM 2770 N LYS B 136 23.203 73.555 111.191 1.00208.87
7
ATOM 2771 CA LYS B 136 21.757 73.613 111.015 1.00208.87
6
ATOM 2772 CB LYS B 136 21.260 75.057 111.134 1.00117.57
6
ATOM 2773 CG LYS B 136 19.750 75.165 111.274 1.00118.63
6
ATOM 2774 CD LYS B 136 19.347 76.462 111.953 1.00119.24
6
ATOM 2775 CE LYS B 136 17.892 76.431 112.384.1.00118.87
6
ATOM 2776 NZ LYS B 136 17.576 77.595 113.252 1.00118.88
7
ATOM 2777 C LYS B 136 21.410 73.054 109.641 1.00208.87
6
ATOM 2778 0 LYS B 136 21.233 71.847 109.488 1.00208.87
8
ATOM 2779 N LEU B 137 21.312 73.935 108.650 1.00 75.67
7
ATOM 2780 CA LEU B 137 21.010 73.521 207.281 1.00 72.95
6
ATOM 2781 CB LEU B 137 22.300 73.140 106.549 1.00114.50
6
ATOM 2782 CG LEU B 137 22.145 72.433 105.196 1.00114.45
6
ATOM 2783 CD1 LEU B 137 21.497 73.373 104.185 1.00114.64
6
ATOM 2784 CD2 LEU B 137 23.512 71.969 104.707 1.00114.47
6
ATOM 2785 C LEU B 137 20.047 72.353 107.172 1.00 72.27
6
ATOM 2786 O LEU B 137 20.452 71.195 107.264 1.00 71.68
8
ATOM 2787 N TYR B 138 18.774 72.660 106.979 1.00 99.33
7
ATOM 2788 CA TYR B 138 17.782 71.615 106.813 1.00 98.44
6
ATOM 2789 CB TYR B 138 16.705 71.682 107.883 1.00101.24
6
ATOM 2790 CG TYR B 138 15.626 70.644 107.666 1.00103.67
6
ATOM 2791 CD1 TYR B 138 15.901 69.283 107.810 1.00105.20
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2792 CE1 TYR B 138 14.911 68.321 107.586 1.00105.71
6
ATOM 2793 CD2 TYR B 138 14.331 71.020 107.291 1.00104.41
6
ATOM 2794 CE2 TYR B 138 13.334 70.065 107.063 1.00104.53
6
ATOM 2795 CZ TYR B 138 13.632 68.720 107.213 1.00105.23
6
ATOM 2796 OH TYR B 138 12.654 67.781 106.988 1.00105.25
8
ATOM 2797 C TYR B 138 17.140 71.823 105.461 1.00 97.46
6
ATOM 2798 O TYR B 138 16.706 72.930 105.141 1.00 98.49
8
ATOM 2799 N MET B 139 17.071 70.760 104.668 1.00 55.68
7
ATOM 2800 CA MET B 139 16.488 70.870 103.343 1.00 53.23
6
ATOM 2801 CB MET B 139 17.575 70.922 102.276 1.00 45.97
6
ATOM 2802 CG MET B 139 18.504 72.111 102.319 1.00 43.14
6
ATOM 2803 SD MET B 139 19.493 72.042 100.831 1.00 40.08
16
ATOM 2804 CE MET B 139 20.758 70.861 101.301 1.00 38.50
6
ATOM 2805 C MET B 139 15.573 69.719 103.008 1.00 53.66
6
ATOM 2806 0 MET B 139 15:803 68.589 103.416 1.00 53.85
8
ATOM 2807 N GLU B 140 14.538 70.027 102.242 1.00 57.95
7
ATOM 2808 CA GLU B 140 13.577 69.038 101.801 1.00 57.39
6
ATOM 2809 CB GLU B 140 12.251 69.169 102.554 1.00 79.51
6
ATOM 2810 CG GLU B 140 12.344 69.115 104.062 1.00 80.34
6
ATOM 2811 CD GLU B 140 10.978 69.016 104.705 1.00 81.16
6
ATOM 2812 OE1 GLU B 140 10.080 69.809 104.340 1.00 81.49
8
ATOM 2813 OE2 GLU B 140 10.800 68.144 105.578 1.00 80.96
8
ATOM 2814 C GLU B 140 13.351 69.381 100.345 1.00 57.83
6
ATOM 2815 O GLU B 140 12.263 69.808 99.966 1.00 58.17
8
ATOM 2816 N VAL B 141 14.389 69.218 99.535 1.00 69.25
7
ATOM 2817 CA VAL B 141 14.301 69.528 98.111 1.00 68.04
6
ATOM 2818 CB VAL B 141 15.729 69.592 97.478 1.00 52.16
6
ATOM 2819 CG1 VAL B 141 16.380 68.213 97.492 1.00 52.26
6
ATOM 2820 CG2 VAL B 141 15.657 70.129 96.069 1.00 52.61
6
ATOM 2821 C VAL B 141 13.435 68.510 97.352 1.00 68.81
6
ATOM 2822 O VAL B 141 13.279 67.368 97.781 1.00 68.33
8
ATOM 2823 N ARG B 142 12.849 68.947 96.242 1.00 88.90
7
ATOM 2824 CA ARG B 142 12.028 68.076 95.412 1.00 89.77
6
ATOM 2825 CB ARG B 142 10.783 68.817 94.911 1.00 81.59
6
ATOM 2826 CG ARG B 142 10.014 68.064 93.832 1.00 85.37
6
ATOM 2827 CD ARG B 142 8.820 68.839 93.298 1.00 88.90
6
ATOM 2828 NE ARG B 142 8.096 68.065 92.287 1.00 92.35
7
ATOM 2829 CZ ARG B 142 6.875 68.355 91.835 1.00 93.50
6
ATOM 2830 NH1 ARG B 142 6.214 69.409 92.298 1.00 93.60
7
ATOM 2831 NH2 ARG B 142 6.312 67.588 90.910 1.00 94.27
7
ATOM 2832 C ARG B 142 12.882 67.669 94.219 1.00 90.42
6
ATOM 2833 O ARG B 142 13.995 68.165 94.050 1.00 91.24
8
ATOM 2834 N VAL B 143 12.369 66.752 93.406 1.00 89.15
7
ATOM 2835 CA VAL B 143 13.062 66.298 92.206 1.00 87.96
6
ATOM 2836 CB VAL B 143 13.926 65.042 92.491 1.00 37.59
6
ATOM 2837 CG1 VAL B 143 14.830 64.755 91.306 1.00 37.23
6
ATOM 2838 CG2 VAL B 143 14.788 65.269 93.739 1.00 36.71
6
ATOM 2839 C VAL B 143 11.955 66.020 91.182 1.00 89.07
6
ATOM 2840 0 VAL B 143 10.777 66.012 91.537 1.00 89.12
8
ATOM 2841 N ASP B 144 12.310 65.795 89.922 1.00 83.46
7
ATOM 2842 CA ASP B 144 11.277 65.607 88.905 1.00 83.97
6
ATOM 2843 CB ASP B 144 10.793 66.988 88.454 1.00 69.07
6
ATOM 2844 CG ASP B 144 9.328 67.011 88.100 1.00 70.86
6
ATOM 2845 OD1 ASP B 144 8.506 67.270 89.006 1.00 71.25
8
ATOM 2846 OD2 ASP B 144 8.998 66.765 86.918 1.00 71.19
8
ATOM 2847 C ASP B 144 11.687 64.807 87.665 1.00 85.08
6
51

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2848 O ASP B 144 12.858 64.501 87.448 1.00 85.87
ATOM 2849 N ARG B 145 10.694 64.491 86.843 1.00 81.25
ATOM 2850 CA ARG B 145 10.897 63.752 85.606 1.00 81.48
ATOM 2851 CB ARG B 145 9.883 62.598 85.526 1.00116.67
ATOM 2852 CG ARG B 145 10.197 61.495 84.513 1.00121.00
ATOM 2853 CD ARG B 145 9.541 61.742 83.154 1.00124.57
ATOM 2854 NE ARG B 145 9.884 60.704 82.178 1.00127.18
ATOM 2855 CZ ARG B 145 9.427 60.664 80.929 1.00128.14
ATOM 2856 NH1 ARG B 145 8.598 61.604 80.485 1.00127.70
ATOM 2857 NH2 ARG B 145 9.800 59.682 80.119 1.00128.38
ATOM 2858 C ARG B 3.45 10.633 64.790 84.517 1.00 80.76
ATOM 2859 0 ARG B 145 9.554 65.390 84.488 1.00 81.40
ATOM 2860 N GLY B 146 11.611 65.015 83.636 1.00 59.75
ATOM 2861 CA GLY B 146 11.434 66.010 82.586 1.00 58.12
ATOM 2862 C GLY B 146 11.790 65.634 81.154 1.00 57.76
ATOM 2863 0 GLY B 146 12.133 64.484 80.855 1.00 57.87
ATOM 2864 N VAL B 147 11.711 66.630 80.271 1.00 55.35
ATOM 2865 CA VAL B 147 11.991 66.453 78.849 1.00 54.41
ATOM 2866 CB VAL B 147 10.865 67.038 77.992 1.00 80.10
ATOM 2867 CG1 VAL B 147 9.570 66.311 78.277 1.00 81.12
ATOM 2868 CG2 VAL B 147 10.715 68.523 78.281 1.00 81.16
ATOM 2869 C VAL B 147 13.293 67.083 78.369 1.00 53.79
ATOM 2870 0 VAL B 147 14.112 66.411 77.744 1.00 54.45
ATOM 2871 N GLY B 148 13.477 68.373 78.635 1.00 56.94
ATOM 2872 CA GLY B 148 14.690 69.041 78.202 1.00 56.02
ATOM 2873 C GLY B 148 15.307 69.932 79.266 1.00 55.80
ATOM 2874 O GLY B 148 16.032 69.462 80.152 1.00 55.58
ATOM 2875 N TYR B 149 15.024 71.229 79.163 1.00 62.76
ATOM 2876 CA TYR B 149 15.519 72.225 80.109 1.00 61.67
ATOM 2877 CB TYR B 149 16.755 72.942 79.544 1.00 47.58
ATOM 2878 CG TYR B 149 17.298 74.030 80.448 1.00 44.27
ATOM 2879 CD1 TYR B 149 18.358 73.778 81.314 1.00 43.41
ATOM 2880 CE1 TYR B 149 18.818 74.761 82.198 1.00 42.32
ATOM 2881 CD2 TYR B 149 16.710 75.302 80.480 1.00 42.67
ATOM 2882 CE2 TYR B 149 17.159 76.292 81.360 1.00 41.68
ATOM 2883 CZ TYR B 149 18.212 76.010 82.219 1.00 42.28
ATOM 2884 OH TYR B 149 18.644 76.950 83.128 1.00 41.87
ATOM 2885 C TYR B 149 14.405 73.240 80.331 1.00 63.16
ATOM 2886 0 TYR B 149 13.682 73.576 79.398 1.00 63.11
ATOM 2887 N VAL B 150 14.255 73.716 81.563 1.00 82.04
ATOM. 2888 CA VAL B 150 13.236 74.716 81.863 1.00 83.70
ATOM 2889 CB VAL B 150 11.893 74.089 82.226 1.00 46.67
ATOM 2890 CG1 VAL B 150 10.820 74.625 81.293 1.00 46.16
ATOM 2891 CG2 VAL B 150 11.979 72.589 82.146 1.00 46.53
ATOM 2892 C VAL B 150 13.656 75.634 82.997 1.00 86.08
ATOM 2893 0 VAL ~ 150 14.025 75.186 84.079 1.00 85.92
ATOM 2894 N PRO B 151 13.597 76.946 82.756 1.00 71.11
ATOM 2895 CD PRO B 151 13.161 77.521 81.472 1.00 58.08
ATOM 2896 CA PRO B 151 13.962 77.999 83.708 1.00 73.95
ATOM 2897 CB PRO ~ 151 13.869 79.267 82.854 1.00 60.58
ATOM 2898 CG PRD B 151 12.799 78.926 81.856 1.00 59.02
ATDM 2899 C PRO B 151 13.101 78.078 84.972 1.00 76.59
ATOM 2900 0 PRO B 151 12.677 77.055 85.511 1.00 76.74
ATOM 2901 N ALA B 152 12.883 79.308 85.444 1.00 39.27
ATDM 2902 CA ALA B 152 12.057 79.598 86.616 1.00 42.47
ATOM 2903 CB ALA B 152 12.676 80.736 87.413 1.00107.83
52

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2904 C ALA B 152 10.635 79.979 86.157 1.00 44.62
6
ATOM 2905 0 ALA B 152 10.154 81.093 86.386 1.00 44.85
8
ATOM 2906 N GLU B 153 9.974 79.035 85.497 1.00173.69
7
ATOM 2907 CA GLU B 153 8.624 79.258 85.006 1.00177.64
6
ATOM 2908 CB GLU B 153 8.640 79.422 83.487 1.00172.29
6
ATOM 2909 CG GLU B 153 7.300 79.820 82.895 1.00175.51
6
ATOM 2910 CD GLU B 153 6.805 81.159 83.414 1.00177.58
6
ATOM 2912 OE1 GLU B 153 7.539 82.160 83.272 1.00179.18
8
ATOM 2912 OE2 GLU B 153 5.681 81.214 83.959 1.00179.05
8
ATOM 2913 C GLU B 153 7.711 78.100 85.399 1.00179.79
6
ATOM 2914 0 GLU B 153 6.486 78.190 85.286 1.00180.54
8
ATOM 2915 N ARG B 154 8.313 77.010 85.863 1.00116.41
7
ATOM 2916 CA ARG B 154 7.545 75.852 86.283 1.00117.36
6
ATOM 2917 CB ARG B 154 8.356 74.572 86.077 1.00160.53
6
ATOM 2918 CG ARG B 154 8.513 74.185 84.614 1.00164.64
6
ATOM 2919 CD ARG B 154 7.156 73.958 83.940 1.00168.10
6
ATOM 2920 NE ARG B 154 7.294 73.684 82.509 1.00170.66
7
ATOM 2921 CZ ARG B 154 6.284 73.387 81.698 1.00271.03
6
ATOM 2922 NH1 ARG B 154 5.046 73.321 82.168 1.00171.38
7
ATOM 2923 NH2 ARG B 154 6.513 73.154 80.412 1.00171.09
7
ATOM 2924 C ARG B 154 7.129 75.992 87.738 1.00116.81
6
ATOM 2925 0 ARG B 154 6.332 75.204 88.240 1.00117.13
8
ATOM 2926 N HIS B 155 7.678 76.997 88.413 1.00 55.66
7
ATOM 2927 CA HIS B 155 7.348 77.274 89.818 1.00 55.25
6
ATOM 2928 CB HIS B 155 5.966 77.938 89.894 1.00208.87
6
ATOM 2929 CG HIS B 155 5.783 79.078 88.934 1.00208.87
6
ATOM 2930 CD2 HIS B 155 6.633 79.645 88.044 1.00208.87
6
ATOM 2931 ND1 HIS B 155 4.588 79.753 88.801 1.00208.87
7
ATOM 2932 CE1 HIS B 155 4.708 80.682 87.870 1.00208.87
6
ATOM 2933 NE2 HIS B 155 5.939 80.638 87.394 1.00208.87
7
ATOM 2934 C HIS B 155 7.390 76.045 90.758 1.00 53.61
6
ATOM 2935 0 HIS B 155 6.705 75.033 90.546 1.00 52.58
8
ATOM 2936 N GLY B 156 8.191 76.165 91.815 1.00 77.00
7
ATOM 2937 CA GLY B 156 8.347 75.084 92.773 1.00 75.17
6
ATOM 2938 C GLY B 156 7.224 74.924 93.780 1.00 74.46
6
ATOM 2939 0 GLY B 156 7.414 75.133 94.983 1.00 73.94
8
ATOM 2940 N ILE B 157 6.056 74.540 93.273 1.00110.69
7
ATOM 2941 CA ILE B 157 4.861 74.317 94.081 1.00109.94
6
ATOM 2942 CB ILE B 157 3.890 73.375 93.328 1.00 96.64
6
ATOM 2943 CG2 ILE B 157 2.576 73.271 94.066 1.00 97.13
6
ATOM 2944 CG1 ILE B 157 3.673 73.884 91.899 1.00 96.85
6
ATOM 2945 CD1 ILE B 157 3.199 75.326 91.800 1.00 97.40
6
ATOM 2946 C ILE B 157 5.211 73.711 95.447 1.00109.72
6
ATOM 2947 0 ILE B 157 5.476 74.440 96.403 1.00109.75
8
ATOM 2948 N LYS B 158 5.204 72-.38195.523 1.00105.17
7
ATOM 2949 CA LYS B 158 5.531 71.639 96.742 1.00104.52
6
ATOM 2950 CB LYS B 158 7.013 71.256 96.743 1.00126.04
6
ATOM 2951 CG LYS B 158 7.335 70.011 95.942 1.00127.28
6
ATOM 2952 CD LYS B 158 6.666 68.780 96.535 1.00127.58
6
ATOM 2953 CE LYS B 158 7.045 67.522 95.767 1.00128.34
6
ATOM 2954 NZ LYS B 158 6.394 66.299 96.313 1.00128.78
'7
ATOM 2955 C LYS B 158 5.211 72.330 98.060 1.00104.50
6
ATOM 2956 0 LYS B 158 4.048 72.604 98.361 1.00104.97
8
ATOM 2957 N ASP B 159 6.265 72.587 98.839 1.00 70.07
7
ATOM 2958 CA ASP B 159 6.186 73.231 100.155 1.00 68.58
. 6
ATOM 2959 CB ASP B 159 7.601 73.320 100.749 1.00 75.47
6
53

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 2960 CG ASP B 159 7.617 73.852 102.174 1.00 76.09
ATOM 2961 OD1 ASP B 159 6.953 73.253 103.054 1.00 76.19
ATOM 2962 OD2 ASP B 159 8.307 74.871 102.412 1.00 76.49
ATOM 2963 C ASP B 159 5.546 74.624 100.102 1.00 68.26
ATOM 2964 0 ASP B 159 6.104 75.593 100.612 1.00 67.90
ATOM 2965 N ARG B 160 4.365 74.697 99.492 1.00 91.05
ATOM 2966 CA ARG B 160 3.605 75.932 99.333 1.00 91.10
ATOM 2967 CB ARG B 160 2.606 76.097 100.489 1.00196.98
ATOM 2968 CG ARG B 160 1.425 77.020 100.169 1.00199.78
ATOM 2969 CD ARG B 160 0.318 76.898 101.215 1.00201.81
ATOM 2970 NE ARG B 160 -0.931 77.522 100.779 1.00202.57
ATOM 2971. CZ ARG B 160 -2.067 77.484 101.471 1.00202.64
ATOM 2972 NH1 ARG B 160 -2.119 76.853 102.637 1.00203.08
ATOM 2973 NH2 ARG B 160 -3.156 78.074 100.997 1.00202.29
ATOM 2974 C ARG B 160 4.508 .77.15699.235 1.00 90.17
ATOM 2975 0 ARG B 160 4.862 77.576 98.131 1.00 90.77
ATOM 2976 N ILE B 161 4.881 77.714 100.388 1.00111.41
ATOM 2977 CA ILE B 161 5.742 78.897 100.453 1.00108.90
ATOM 2978 CB ILE B 161 5.186 79.910 101.467 1.00155.30
ATOM 2979 CG2 ILE B 161 5.920 81.240 101.329 1.00155.26
ATOM 2980 CG1 ILE B 161 3.689 80.101 101.221 1.00156.01
ATOM 2981 CD1 ILE B 161 2.979 80.897 102.286 1.00157.04
ATOM 2982 C ILE B 161 7.184 78.537 100.831 1.00106.94
ATOM 2983 O ILE B 161 7.423 77.547 101.521 1.00106.37
ATOM 2984 N ASN B 162 8.131 79.360 100.381 1.00157.69
ATOM 2985 CA ASN B 162 9.562 79.144 100.613 1.00155.50
ATOM 2986 CB ASN B 162 9.848 78.754 102.065 1.00120.56
ATOM 2987 CG ASN B 162 9.259 79.725 103.054 1.00120.85
ATOM 2988 OD1 ASN B 162 9.299 80.936 102.847 1.00120.63
ATOM 2989 ND2 ASN B 162 8.715 79.201 104.148 1.00121.34
ATOM 2990 C ASN B 162 9.993 78.014 99.692 1.00154.09
ATOM 2991 O ASN B 162 10.002 76.847 100.086 1.00154.31
ATOM 2992 N ALA B 163 10.354 78.364 98.463 1.00 77.76
ATOM 2993 CA ALA B 163 10.735 77.354 97.496 1.00 74.56
ATOM 2994 CB ALA B 163 9.571 77.121 96.541 1.00 55.98
ATOM 2995 C ALA B 163 12.007 77.639 96.703 1.00 72.69
ATOM 2996 0 ALA B 163 12.746 76.715 96.355 1.00 73.43
ATOM 2997 N ILE B 164 12.274 78.904 96.410 1.00 59.09
ATOM 2998 CA ILE B 164 13.459 79.242 95.626 1.00 57.08
ATOM 2999 CB ILE B 164 14.715 79.360 96.542 1.00 43.29
ATOM 3000 CG2 ILE B 164 14.450 80.364 97.641 1.00 43.45
ATOM 3001 CG1 ILE B 164 15.046 78.026 97.206 1.00 42.16
ATOM 3002 CD1 ILE B 164 16.244 78.112 98.133 1.00 41.64
ATOM 3003 C ILE B 164 13.681 78.188 94.520 1.00 55.27
ATOM 3004 O ILE B 164 14.807 77.762 94.253 1.00 55.18
ATOM 3005 N PRO B 165 12.590 77.792 93.835 1.00 54:05
ATOM 3006 CD PRO B 165 11.340 78.566 93.734 1.00 45.24
ATOM 3007 CA PRO B 165 12.634 76.794 92.768 1.00 53.19
ATOM 3008 CB PRO B 165 11.277 76.955 92.083 1.00 44.74
ATOM 3009 CG PRO B 165 10.973 78.380 92.281 1.00 44.95
ATOM 3010 C PRO B 165 13.781 76.948 91.798 1.00 51.81
ATOM 3011 0 PRO B 165 13.890 77.945 91.093 1.00 51.05
ATOM 3012 N VAL B 166 14.638 75.941 91.773 1.00 53.76
ATOM 3013 CA VAL B 166 15.772 75.942 90.877 1.00 52.63
ATOM 3014 CB VAL B 166 16.795 74.880 91.303 1.00 37.36
ATOM 3015 CG1 VAL B 166 17.762 75,491 92.294 1.00 37.27
54

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3016 CG2 VALB 166 16.088 73.698 91.955 1.00 37.18
ATOM 3017 C VALB 166 15.295 75.691 89.452 1.00 53.14
ATOM 3018 0 VALB 166 14.093 75.552 89.214 1.00 52.50
ATOM 3019 N ASPB 167 16.228 75.648 88.507 1.00 51.01
ATOM 3020 CA ASPB 167 15.860 75.429 87.116 1.00 51.58
ATOM 3021 CB ASPB 167 16.843 76.149 86.193 1.00 56.28
ATOM 3022 CG ASPB 167 16.933 77.631 86.490 1.00 56.37
ATOM 3023 OD1 ASPB 167 15.857 78.266 86.604 1.00 56.07
ATOM 3024 OD2 ASPB 167 18.067 78.157 86.608 1.00 55.57
ATOM 3025 C ASPB 167 15.785 73.942 86.771 1.00 52.93
ATOM 3026 0 ASPB 167 16.523 73.117 87.316 1.00 54.06
ATOM 3027 N ALAB 168 14.880 73.612 85.860 1.00 70.74
ATOM 3028 CA ALAB 168 14.660 72.237 85.446 1.00 72.04
ATOM 3029 CB ALAB 168 13.238 72.089 84.963 1.00 93.07
ATOM 3030 C ALAB 168 15.621 71.764 84.368 1.00 73.25
ATOM 3031 0 ALAB 168 15.318 71.848 83.176 1.00 74.88
ATOM 3032 N ILEB 169 16.774 71.254 84.784 1.00 40.64
ATOM 3033 CA ILEB 169 17.757 70.769 83.832 1.00 41.50
ATOM 3034 CB ILEB 169 19.213 71.068 84.313 1.00 98.30
ATOM 3035 CG2 ILEB 169 19.539 72.525 84.130 1.00 97.49
ATOM 3036 CG1 ILEB 169 19.381 70.705 85.793 1.00100.66
ATOM 3037 CD1 ILEB 169 19.738 69.249 86.070 1.00102.42
ATOM 3038 C ILEB 169 17.619 69.273 83.586 1.00 41.74
ATOM 3039 0 ILEB 169 18.592 68.532 83.729 1.00 42.04
ATOM 3040 N PHEB 170 16.432 68.802 83.211 1.00 42.29
ATOM 3041 CA PHEB 170 16.317 67.361 82.977 1.00 43.20
ATOM 3042 CB PHEB 170 15.013 66.777 83.538 1.00 61.40
ATOM 3043 CG PHEB 170 13.954 67.786 83.836 1.00 63.03
ATOM 3044 CD1 PHEB 170 13.399 68.556 82.827 1.00 63.24
ATOM 3045 CD2 PHEB 170 13.438 67.889 85.124 1.00 63.04
ATOM 3046 CE1 PHEB 170' 12.338 69.403 83.094 1.00 62.61
-ATOM 3047 CE2 PHEB 170 12.381 68.732 85.398 1.00 62.50
ATOM 3048 CZ PHEB 170 11.827 69.489 84.379 1.00 62.13
ATOM 3049 C PHEB 170 16.502 66.878 81.545 1.00 42.83
ATOM 3050 0 PHEB 170 15.619 67.031 80.700 1.00 42.08
ATOM 3051 N SERB 171 17.672 66.276 81.318 1.00 58.08
ATOM 3052 CA SERB 171 18.105 65.721 80.035 1.00 57.84
ATOM 3053 CB SERB 171 18.167 66.810 78.959 1.00 64.81
ATOM 3054 OG SERB 171 16.915 67.437 78.764 1.00 66.33
ATOM 3055 C SERB 171 19.519 65.173 80.243 1.00 58.17
ATOM 3056 0 SERB 171 20.482 65.929 80.186 1.00 58.94
ATOM 3057 N PROB 172 19.663 63.858 80.480 1.00 62.96
ATOM 3058 CD PROB 172 18.555 62.888 80.478 1.00125.39
ATOM 3059 CA PROB 172 20.948 63.178 80.702 1.00 62.05
ATOM 3060 CB PROB 172 20.586 61.714 80.509 1.00124.61
ATOM 3061 CG PROB 172 19.206 61.658 81.068 1.00125.56
ATOM 3062 C PROB 172 22.105 63.621 79.791 1.00 62.13
ATOM 3063 O PROB 172 23.274 63.464 80.141 1.00 62.81
ATOM 3064 N VALB 173 21.774 64.151 78.617 1.00 50.25
ATOM 3065 CA VALB 173 22.775 64.643 77.679 1.00 49.25
ATOM 3066 CB VALB 173 22.246 64.633 76.232 1.00 47.86
ATOM 3067 CG1 VALB 173 21.865 63.242 75.819 1.00'46.05
ATOM 3068 CG2 VALB 173 21.033 65.531 76.123 1.00 48.20
ATOM 3069 C VALB 173 22.992 66.093 78.087 1.00 50.28
ATOM 3070 0 VALB 173 22.022 66.787 78.407 1.00 50.57
ATOM 3071 N ARGB 174 24.238 66.565 78.080 1.00 46.53

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3072 CA ARGB 174 24.500 67.957 78.459 1.00 45.95
6
ATOM 3073 CB ARGB 174 25.016 68.000 79.903 1.0'076.44
6
ATOM 3074 CG ARGB 174 23.959 67.598 80.932 1.00 76.70
6
ATOM 3075 CD ARGB 174 24.453 67.729 82.365 '1.0077.00
6
ATOM 3076 NE ARGB 174 23.374 67.503 83.323 1.00 77.86
7
ATOM 3077 ~CZ ARGB 174 23.543 67.416 84.639 1.00 78.64
6
ATOM 3078 NH1 ARGB 174 24.754 67.535 85.169 1.00 79.52
7
ATOM 3079 NH2 ARGB 174 22.497 67.208 85.429 1.00 78.53
7
ATOM 3080 C ARGB 174 25.447 68.725 77.531 1.00 45.02
6
ATOM 3081 0 ARGB 174 26.133 69.643 77.966 1.00 45.18
8
ATOM 3082 N ARGB 175 25.449 68.359 76.251 1.00 30.81
7
ATOM 3083 CA ARGB 175 26.307 68.974 75.232 1.00 30.24
6
ATOM 3084 CB ARGB 175 27.705 69.252 75.796 1.00 56.73
6
ATOM 3085 CG ARGB 175 28.609 69.963 74.814 1.00 58.11
6
ATOM 3086 CD ARGB 175 30.066 69.866 75.184 1.00 58.93
6
ATOM 3087 NE ARGB 175 30.879 70.341 74.074 1.00 60.67
7
ATOM 3088 CZ ARGB 175 32.160 70.044 73.914 1.00 61.35
6
ATOM 3089 NH1 ARGB 175 32.771 69.274 74.800 1.00 62.34
7
ATOM 3090 NH2 ARGB 175 32.821 70.495 72.859 1.00 61.62
7
ATOM 3091 C ARGB 175 26.434 68.025 74.030 1.00 29.61
6
ATOM 3092 0 ARGB 175 26.804 66.867 74.174 1.00 28.19
8
ATOM 3093 N VALB 176 26.163 68.521 72.837 1.00 43.00
7
ATOM 3094 CA VALB 176 26.209 67.663 71.674 1.00 42.87
6
ATOM 3095 CB VALB 176 24.779 67.226 71.310 1.00 59.69
6
ATOM 3096 CG1 VALB 176 24.808 66.209 70.197 1.00 61.48
6
ATOM 3097 CG2 VALB 176 24.080 66.679 72.535 1.00 59.65
6
ATOM 3098 C VALB 176 26.838 68.321 70.450 1.00 43.44
6
ATOM 3099 O VALB 176 26.225 69.206 69.832 1.00 43.32
8
ATOM 3100 N ALAB 177 28.047 67.883 70.093 1.00 43.93
7
ATOM 3101 CA ALAB 177 28.750 68.414 68.917 1.00 44.13
6
ATOM 3102 CB ALAB 177 30.096 69.001 69.326 1.00 84.37
6
ATOM 3103 C ALAB 177 28.946 67.333 67.846 1.00 43.58
6
ATOM 3104 0 ALAB 177 28.846 66.139 68.127 1.00 43.19
8
ATOM 3105 N PHEB 178 29.236 67.754 66.618 1.00 16.23
7
ATOM 3106 CA PHEB 178 29.413 66.806 65.523 1.00 17.34
6
ATOM 3107 CB PHEB 178 28.079 66.114 65.248 1.00 28.38
6
ATOM 3108 CG PHEB 178 27.105 66.970 64.492 1.00 26.31
6
ATOM 3109 CD1 PHEB 178 27.007 66.878 63.110 1.00 25.18
6
ATOM 3110 CD2 PHEB 178 26.323 67.910 65.151 1.00 24.98
6
ATOM 3111 CE1 PHEB 178 26.145 67.713 62.393 1.00 23.37
6
ATOM 3112 CE2 PHEB 178 25.458 68.754 64.434 1.00 23.56
6
ATOM 3113 CZ PHEB 178 25.370 68.653 63.057 1.00 22.91
6
ATOM 3114 C PHEB 178 29.912 67.458 64.227 1.00 18.91
6
ATOM 3115 O PHEB 178 29.345 68.451 63.769 1.00 17.56
8
ATOM 3116 N GLNB 179 30.963 66.908 63.625 1.00 62.17
7
ATOM 3117 CA GLNB 179 31.449 67.473 62.369 1.00 65.93
6
ATOM 3118 CB GLNB 179 32.773 68.234 62.560 1.00 80.10
6
ATOM 3119 CG GLNB 179 33.982 67.411 62.962 1.00 81.43
6
ATOM 3120 CD GLNB 179 35.287 68.139 62.658 1.00 82.38
6
ATOM 3121 OE1 GLNB 179 35.333 69.371 62.650 1.00 82.70
8
ATOM 3122 NE2 GLNB 179 36.355 67.379 62.417 1.00 81.97
7
ATOM 3123 C GLNB 179 31.606 66.428 61.282 1.00 66.88
6
ATOM 3124 0 GLNB 179 32.281 65.425 61.468 1.00 67.65
8
ATOM 3225 N VALB 180 30.979 66.674 60.140 1.00 30.90
7
ATOM 3126 CA VALB 180 31.039 65.738 59.032 1.00 33.56
6
ATOM 3127 CB VALB 180 29.649 65.640 58.339 1.00 68.73
6
56

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3128 CG1 VALB 180 29.190 67.013 57.885 1.00 69.38
6
ATOM 3129 CG2 VALB 180 29.706 64.663 57.172 1.00 69.19
6
ATOM 3130 C VALB 180 32.127 66.030 57.990 1.00 35.36
6
ATOM 3131 0 VALB 180 31.912 66.779 57.033 1.00 35.29
8
ATOM 3132 N GLUB 181 33.296 65.428 58.168 1.00 62.66
7
ATOM 3133 CA GLUB 181 34.383 65.627 57.219 1.00 65.99
6
ATOM 3134 CB GLUB 181 35.724 65.329 57.896 1.00120.88
6
ATOM 3135 CG GLUB 181 36.953 65.566 57.025 1.00123.47
6
ATOM 3136 CD GLUB 181 38.245 65.511 57.823 1.00124.36
6
ATOM 3137 OE1 GLUB 181 38.399 64.565 58.628 1.00123.76
8
ATOM 3238 OE2 GLUB 181 39.108 66.402 57.644 1.00125.32
8
ATOM 3139 C GLUB 181 34.175 64.714 56.004 1.00 67.27
6
ATOM 3140 0 GLUB 181 33.316 63.842 56.019 1.00 68.13
8
ATOM 3141 N ASPB 182 34.943 64.921 54.945 1.00 43.73
7
ATOM 3142 CA ASPB 182 34.808 64.091 53.763 1.00 45.68
6
ATOM 3143 CB ASPB 182 35.001 64.914 52.486 1.00 97.05
6
ATOM 3144 CG ASPB 182 34.684 66.383 52.677 1.00 98.74
6
ATOM 3145 OD1 ASPB 182 33.808 66.704 53.506 1.00100.74
8
ATOM 3146 OD2 ASPB 182 35.302 67.218 51.983 1.00 97.37
8
ATOM 3147 C ASPB 182 35.875 63.008 53.816 1.00 47.05
6
ATOM 3148 0 ASPB 182 36.967 63.237 54.333 1.00 47.61
8
ATOM 3149 N THRB 183 35.548 61.832 53.285 1.00 51.94
7
ATOM 3150 CA THRB 183 36.461 60.686 53.225 1.00 53.53
6
ATOM 3151 CB THRB 183 36.225 59.684 54.366 1.00 71.65
6
ATOM 3152 OG1 THRB 183 34.860 59.248 54.340 1.00 71.78
8
ATOM 3153 CG2 THRB 183 36.547 60.318 55.704 1.00 72.82
6
ATOM 3154 C THRB 183 36.140 59.993 51.922 1.00 54.41
6
ATOM 3155 0 THRB 183 34.991 60.012 51.479 1.00 54.35
8
ATOM 3156 N ALAB 184 37.137 59.360 51.319 1.00129.81
7
ATOM 3157 CA ALAB 184 36.918 58.709 50.042 1.00131.82
6
ATOM 3158 CB ALAB 184 37.776 59.367 48.990 1.00 58.25
6
ATOM 3159 C ALAB 184 37.140 57.215 49.991 1.00133.33
6
ATOM 3160 0 ALAB 184 37.857 56.745 49.109 1.00134.76
8
ATOM 3161 N LEUB 185 36.553 56.454 50.911 1.00148.14
7
ATOM 3162 CA LEUB 185 36.730 55.008 50.835 1.00148.94
6
ATOM 3163 CB LEUB 185 35.855 54.272 51.850 1.00 94.82
6
ATOM 3164 CG LEUB 185 36.328 54.260 53.306 1.00 94.69
6
ATOM 3165 CD1 LEUB 185 35.483 53.256 54.075 1.00 94.46
6
ATOM 3166 CD2 LEUB 185 37.801 53.882 53.387 1.00 93.85
6
ATOM 3167 C LEUB 185 36.304 54.652 49.419 1.00149.86
6
ATOM 3168 0 LEUB 185 37.106 54.147 48.629 1.00150.21
8
ATOM 3169 N GLYB 186 35.041 54.921 49.093 1.00 67.38
7
ATOM 3170 CA GLYB 186 34.596 54.663 47.741 1.00 68.06
6
ATOM 3171 C GLYB 186 35.455 55.625 46.944 1.00 68.15
6
ATOM 3172 0 GLYB 186 35.579 56.778 47.356 1.00 68.60
8
ATOM 3173 N GLNB 187 36.064 55.171 45.847 1.00110.85
7
ATOM 3174 CA GLNB 187 36.927 56.015 45.008 1.00110.89
6
ATOM 3175 CB GLNB 187 36.852 55.563 43.549 1.00 71.69
6
ATOM 3176 CG GLNB 187 37.833 56.277 42.633 1.00 70.62
6
ATOM 3177 CD GLNB 187 37.348 57.617 42.111 1.00 70.79
6
ATOM 3178 OE1 GLNB 187 36.859 57.716 40.986 1.00 71.50
8
ATOM 3179 NE2 GLNB 187 37.485 58.654 42.924 1.00 70.20
7
ATOM 3180 C GLNB 187 36.619 57.510 45.069 1.00110.45
6
ATOM 3181 0 GLNB 187 37.488 58.312 45.414 1.00110.76
8
ATOM 3182 N ARGB 188 35.394 57.877 44.694 1.00 83.21
7
ATOM 3183 CA ARGB 188 34.936 59.265 44.715 1.00 82.05
6
57

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3184 CB ARG B 188 33.412 59.298 44.738 1.00138.62
6
ATOM 3185 CG ARG B 188 32.820 60.676 44.639 1.00139.12
6
ATOM 3186 CD ARG B 188 31.322 60.625 44.855 1.00139.80
6
ATOM 3187 NE ARG B 188 30.722 61.944 44.704 1.00141.03
7
ATOM 3188 CZ ARG B 188 29.450 62.221 44.971 1.00141.96
6
ATOM 3189 NH1 ARG B 188 28.639 61.266 45.409 1.00143.04
7
ATOM 3190 NH2 ARG B 188 28.989 63.454 44.800 1.00141.62
7
ATOM 3291 C ARG B 188 35.491 59.892 45.985 2.00 80.56
6
ATOM 3192 0 ARG B 188 35.553 59.236 47.024 1.00 81.23
8
ATOM 3193 N THR B 189 35.878 61.161 45.915 1.00 77.04
7
ATOM 3194 CA THR B 189 36.477 61.828 47.067 1.00 74.63
6
ATOM 3195 CB THR B 189 37.717 62.551 46.638 1.00 59.96
6
ATOM 3196 OG1 THR B 189 37.354 63.515 45.645 1.00 60.93
8
ATOM 3197 CG2 THR B 189 38.713 61.575 46.050 1.00 59.93
6
ATOM 3198 C THR B 189 35.638 62.826 47.854 1.00 71.92
6
ATOM 3199 O THR B 189 ~ 36.171 63.526 48.716 1.00 72.50
8
ATOM 3200 N ASP B 190 34.341 62.905 47.576 1.00 63.78
7
ATOM 3201 CA ASP B 190 33.491 63.841 48.297 1.00 61.55
6
ATOM 3202 CB ASP B 190 32.921 64.882 47.330 1.00 77.28
6
ATOM 3203 CG ASP B 190 32.553 64.292 45.994 1.00 78.99
6
ATOM 3204 OD1 ASP B 190 33.453 63.761 45.309 1.00 79.32
8
ATOM 3205 OD2 ASP B 190 31.365 64.365 45.625 1.00 $0.25
8
ATOM 3206 C ASP B 190 32.366 63.199 49.099 1.00 58.55
6
ATOM 3207 0 ASP B 190 31.262 63.725 49.159 1.00 59.65
8
ATOM 3208 N LEU B 191 32.649 62.076 49.741 1.00 74.59
7
ATOM 3209 CA LEU B 192 31.628 61.427 50.530 1.00 72.86
6
ATOM 3210 CB LEU B 191 31.870 59.915 50.490 1.00 39.25
6
ATOM 3211 CG LEU B 191 31.700 59.458 49.027 1.00 38.40
6
ATOM 3212 CD1 LEU B 191 32.178 58.033 48.832 1.00 37.17
6
ATOM 3213 CD2 LEU B 191 30.238 59.588 48.622 1.00 37.38
6
ATOM 3214 C LEU B 191 31.610 61.981 51.953 1.00 70.45
6
ATOM 3215 0 LEU B 191 32.562 62.647 52.357 1.00 72.17
8
ATOM 3216 N ASP B 192 30.523 61.740 52.696 1.00 39.62
7
ATOM 3217 CA ASP B 192 30.354 62.265 54.068 1.00 36.40
6
ATOM 3218 CB ASP B 192 28.898 62.701 54.282 1.00 78.82
6
ATOM 3219 CG ASP B 192 28.578 64.025 53.601 1.00 79.38
6
ATOM 3220 OD1 ASP B 192 29.164 64.299 52.532 1.00 79.92
8
ATOM 3221 OD2 ASP B 192 27.735 64.783 54.131 1.00 79.32
8
ATOM 3222 C ASP B 192 30.773 61.330 55.199 1.00 32.27
6
ATOM 3223 O ASP B 192 30.658 60.115 55.081 1.00 31.34
8
ATOM 3224 N LYS B 193 31.248 61.917 56.294 1.00 21.17
7
ATOM 3225 CA LYS B 193 31.718 61.163 57.450 1.00 18.63
6
ATOM 3226 CB LYS B 193 33.241 61.060 57.446 1.00 54.65
6
ATOM 3227 CG LYS B 193 33.820 60.353 58.652 1.00 55.49
6
ATOM 3228 CD LYS B 193 34.597 59.149 58.199 1.00 58.46
6
ATOM 3229 CE LYS B 193 35.959 59.038 58.888 1.00 60.61
6
ATOM 3230 NZ LYS B 193 36.881 58.045 58.209 1.00 62.12
7
ATOM 3231 C LYS B 193 31.264 61.796 58.757 1.00 16.86
6
ATOM 3232 O LYS B 193 32.071 62.340 59.534 1.00 15.25
8
ATOM 3233 N LEU B 194 29.960 61.671 58.985 1.00 56.76
7
ATOM 3234 CA LEU B 194 29.248 62.189 60.146 1.00 54.94
6
ATOM 3235 CB LEU B 194 28.004 61.332 60.376 1.00 26.43
6
ATOM 3236 CG LEU B 194 26.860 61.933 61.191 1.00 23.96
6
ATOM 3237 CD1 LEU B 194 26.960 61.449 62.612 2.00 23.82
6
ATOM 3238 CD2 LEU B 194 26.879 63.450 61.118 1.00 22.05
6
ATOM 3239 C LEU B 194 29.995 62.384 61.468 1.00 54.76
6
58

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3240 0 LEU B 194 , 30.366 63.506 61.799 1.00 55.92
8
ATOM 3241 N THR B 195 30.211 61.316 62.227 1.00 24.80
7
ATOM 3242 CA THR B 195 30.885 61.427 63.529 1.00 24.82
6
ATOM 3243 CB THR B 195 32.305 62.008 63.430 1.00 13.87
6
ATOM 3244 OG1 THR B 195 33.111 61.165 62.607 1.00 13.87
8
ATOM 3245 CG2 THR B 195 32.929 62.113 64.826 1.00 13.87
6
ATOM 3246 C THR B 195 30.127 62.323 64.513 1.00 27.11
6
ATOM 3247 0 THR B 195 29.933 63.510 64.266 1.00 27.79
8
ATOM 3248 N LEU B 196 29.710 61.748 65.634 1.00 83.35
7
ATOM 3249 CA LEU B 196 28.987 62.491 66.655 1.00 85.23
6
ATOM 3250 CB LEU B 196 27.600 61.889 66.865 1.00 37.74
6
ATOM 3251 CG LEU B 196 26.427 62.775 66.443 1.00 37.19
6
ATOM 3252 CD1 LEU B 196 26.607 63.276 65.031 1.00 35.58
6
ATOM 3253 CD2 LEU B 196 25.156 61.986 66.537 1.00 38.11
6
ATOM 3254 C LEU B 196 29.779 62.409 67.941 1.00 88.34
6
ATOM 3255 0 LEU B 196 30.801 61.729 67.998 1.00 90.29
8
ATOM 3256 N ARG B 197 29.316 63.111 68.968 1.00 85.67
7
ATOM 3257 CA ARG B 197 29.977 63.095 70.267 1.00 87.64
6
ATOM 3258 CB ARG B 197 31.277 63.895 70.208 1.00 65.93
6
ATOM 3259 CG ARG B 197 31.185 65.241 69.531 1.00 68.55
6
ATOM 3260 CD ARG B 197 32.584 65.793 69.339 1.00 73.98
6
ATOM 3261 NE ARG B 197 32.618 67.245 69.442 1.00 77.56
7
ATOM 3262 CZ ARG B 197 33.720 67.948 69.682 1.00 79.42
6
ATOM 3263 NH1 ARG B 197 34.881 67.328 69.841 1.00 81.24
7
ATOM 3264 NH2 ARG B 197 33.659 69.269 69.778 1.00 79.66
7
ATOM 3265 C ARG B 197 29.020 63.642 71.314 1.00 89.08
6
ATOM 3266 0 ARG B 197 28.980 64.840 71.597 1.00 90.63
8
ATOM 3267 N ILE B 198 28.251 62.718 71.880 1.00 28.99
7
ATOM 3268 CA ILE B 198 27.217 63.003 72.861 1.00 29.28
6
ATOM 3269 CB ILE B 198 26.045 61.980 72.665 1.00 51.66
6
ATOM 3270 CG2 ILE B 198 25.714 61.278 73.933 1.00 52.32
6
ATOM 3271 CG1 ILE B 198 24.805 62.683 72.114 1.00 52.64
6
ATOM 3272 CD1 ILE B 198 24.873 63.007 70.646 1.00 55.60
6
ATOM 3273 C ILE B 198 27.731 63.000 74.299 1.00 30.75
6
ATOM 3274 0 ILE B 198 28.034 61.955 74.852 1.00 30.87
8
ATOM 3275 N TRP B 199 27.847 64.179 74.901 1.00 48.14
7
ATOM 3276 CA TRP B 199 28.312 64,280 76.282 1.00 50.03
6
ATOM 3277 CB TRP B 199 29.041 65.602 76.544 1.00 51.23
6
ATOM 3278 CG TRP B 199 30.115 65.946 75.560 1.00 52.27
6
ATOM 3279 CD2 TRP B 199 31.525 65.840 75.762 1.00 52.01
6
ATOM 3280 CE2 TRP B 199 32.149 66.243 74.564 1.00 51.99
6
ATOM 3281 CE3 TRP B 199 32.324 65.440 76.837 1.00 52.21
6
ATOM 3282 CD1 TRP B 199 29.946 66.401 74.282 1.00 52.95
6
ATOM 3283 NE1 TRP B 199 31.161 66.582 73.678 1.00 52.35
7
ATOM 3284 CZ2 TRP B 199 33.540 66.255 74.411 1.00 51.93
6
ATOM 3285 CZ3 TRP B 199 33.710 65.452 76.683 1.00 52.41
6
ATOM 3286 CH2 TRP B 199 34.301 65.856 75.480 1.00 51.86
6
ATOM 3287 C TRP B 199 27.076 64,235 77.158 1.00 51.99
6
ATOM 3288 0 TRP B 199 26.265 65.164 77.14 1.00 52.37
5 8
ATOM 3289 N THR B 200 26.928 63.155 77.920 1.00 95.11
7
ATOM 3290 CA THR B 200 25.770 63.002 78.797 1.00 96.45
6
ATOM 3291 CB THR B 200 24.694 62.115 78.166 1.00148.63
6
ATOM 3292 OG1 THR B 200 24.987 60.742 78.445 1.00149.74
8
ATOM 3293 CG2 THR B 200 24.666 62,310 76.678 1.00147.62
6
ATOM 3294 C THR B 200 26.150 62,350 80.113 1.00 96.31
6
ATOM 3295 O THR B 200 26.988 61.452 80.152 1.00 96.11
8
59

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3296 N ASP B 201 25.522 62.810 81.189 1.00 43.75
ATOM 3297 CA ASP B 201 25.759 62.247 82.514 1.00 44.52
ATOM 3298 CB ASP B 201 27.101 62.721 83.083 1.00 82.69
ATOM 3299 CG ASP B 201 27.651 61.776 84.145 1.00 84.02
ATOM 3300 OD1 ASP B 201 26.866 61.333 85.011 1.00 85.42
ATOM 3301 OD2 ASP B 201 28.868 61.480 84.120 1.00 84.72
ATOM 3302 C ASP B 201 24.615 62.631 83.446 1.00 44.46
ATOM 3303 O ASP B 201 24.623 62.293 84.631 1.00 44.19
ATOM 3304 N GLY B 202 23.624 63.329 82.898 1.00 75.55
ATOM 3305 CA GLY B 202 22.477 63.706 83.692 1.00 75.47
ATOM 3306 C GLY B 202 21.719 62.453 $4.110 1.00 76.50
ATOM 3307 0 GLY B 202 20.547 62.549 84.483 1.00 77.97
ATOM 3308 N SER B 203 22.392 61.292 84.035 1.00 55.59
ATOM 3309 CA SER B 203 21.867 59.957 $4.392 1.00 54.17
ATOM 3310 CB SER B 203 20.356 .59.99384.676 1.00 91.62
ATOM 3311 OG SER B 203 19.605 60.381 83.538 1.00 90.27
ATOM 3312 C SER B 203 22.136 58.886 83.325 1.00 52.93
ATOM 3313 O SER B 203 23.059 58.092 83.461 1.00 53.08
ATOM 3314 N VAL B 204 21.309 58.875 82.277 1.00 69.60
ATOM 3315 CA VAL B 204 21.390 57.927 81.154 1.00 68.03
ATOM 3316 CB VAL B 204 20.338 58.242 $0.108 1.00 35.10
ATOM 3317 CG1 VAL B 204 20.400 57.231 78.991 1.00 34.10
ATOM 3318 CG2 VAL B 204 18.985 58.275 80.746 1.00 34.56
ATOM 3319 C VAL B 204 22.709 57.931 80.407 1.00 67.75
ATOM 3320 O VAL B 204 23.357 58.962 80.309 1.00 69.06
ATOM 3321 N THR B 205 23.082 56.793 79.829 1.00 49.09
ATOM 3322 CA THR B 205 24.340 56.711 79.093 1.00 48.85
ATOM 3323 CB THR B 205 24.767 55.263 78.850 1.00 77.86
ATOM 3324 OG1 THR B 205 24.007 54.721 77.768 1.00 79.05
ATOM 3325 CG2 THR B 205 24.542 54.429 80.092 1.00 78.88
ATOM 3326 C THR B 205 24.225 57.400 77.737 1.00 49.21
ATOM 3327 0 THR B 205 23.131 57.794 77.327 1.00 49.54
ATOM 3328 N PRO B 206 25.361 57.556 77.023 1.00 46.13
ATOM 3329 CD PRO B 206 26.729 57.238 77.468 1.00 48.07
ATOM 3330 CA PRO B 206 25.402 58.200 75.707 1.00 45.85
ATOM 3331 CB PRO B 206 26.904 58.299 75.415 1.00 48.63
ATOM 3332 CG PRO B 206 27.534 58.294 76.752 1.00 48.13
ATOM 3333 C PRO B 206 24.674 57.407 74.626 1.00 47.15
ATOM 3334 O PRO B 206 23.585 57.780 74.189 1.00 46.48
ATOM 3335 N LEU B 207 25.304 56.318 74.191 1.00 76.94
ATOM 3336 CA LEU B 207 24.746 55.460 73.155 1.00 77.75
ATOM 3337 CB LEU B 207 25.444 54.099 73.169 1.00101.16
ATOM 3338 CG LEU B 207 25.120 53.141 72.022 1.00101.47
ATOM 3339 CD1 LEU B 207 25.360 53.807 70.671 1.00101.74
ATOM 3340 CD2 LEU B 207 25.988 51.910 72.173 1.00101.71
ATOM 3341 C LEU B 207 23.252 55.303 73.390 1.00 78.54
ATOM 3342 O LEU B 207 22.470 55.176 72.446 1.00 78.25
ATOM 3343 N GLU B 208 22.864 55.323 74.660 1.00 60.01
ATOM 3344 CA GLU B 208 21.461 55.218 75.023 1.00 61.02
ATOM 3345 CB GLU B 208 21.302 55.117 76.546 1.00128.03
ATOM 3346 CG GLU B 208 21.543 53.714 77.097 1.00131.02
ATOM 3347 CD GLU B 208 21.287 53.608 78.591 1.00133.34
ATOM 3348 OE1 GLU B 208 20.215 54.062 79.041 1.00134.35
ATOM 3349 OE2 GLU B 208 22.149 53.060 79.315 1.00134.47
ATOM 3350 C GLU B 208 20.742 56.458 74.497 1.00 60.26
ATOM 3351 0 GLU B 208 19.750 56.360 73.769 1.00 58.68

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3352 N ALA B 209 21.249 57.625 74.865 1.00 65.53
7
ATOM 3353 CA ALA B 209 20.653 58.857 74.402 1.00 65.98
6
ATOM 3354 CB ALA B 209 21.552 60.033 74.735 1.00 16.71
6
ATOM 3355 C ALA B 209 20.453 58.740 72.891 1.00 66.92
6
ATOM 3356 0 ALA B 209 19.319 58.605 72.431 1.00 67.52
8
ATOM 3357 N LEU B 210 21.543 58.756 72'.123 1.00 73.15
7
ATOM 3358 CA LEU B 210 21.443 58.668 70.662 1.00 73.45
6
ATOM 3359 CB LEU B 210 22.777 58.207 70.051 1.00 36.69
6
ATOM 3360 CG LEU B 210 22.693 57.718 68.591 2.00 36.68
6
ATOM 3361 CD1 LEU B 210 22.266 58.832 67.646 1.00 36.19
6
ATOM 3362 CD2 LEU B 210 24.030 57.169 68.179 1.00 37.72
6
ATOM 3363 C LEU B 210 20.312 57.752 70.178 1.00 73.95
6
ATOM 3364 0 LEU B 210 19.323 58.223 69.611 1.00 73.49
8
ATOM 3365 N ASN B 211 20.464 56.450 70.414 1.00 95.72
7
ATOM 3366 CA ASN B 211 19.475 55.452 70.005 1.00 94.91
6
ATOM 3367 CB ASN B 211 19.789 54.099 70.646 1.00 47.61
6
ATOM 3368 CG ASN B 211 20.941 53.388 69.969 1.00 48.99
6
ATOM 3369 OD1 ASN B 211 20.855 53.047 68.782 1.00 49.54
8
ATOM 3370 ND2 ASN B 211 22.025 53.156 70.711 1.00 49.80
7
ATOM 3371 C ASN B 211 18.065 55.862 70.378 1.00 94.05
6
ATOM 3372 0 ASN B 211 17.183 55.935 69.524 1.00 94.10
8
ATOM 3373 N GLN B 212 17.863 56.119 71.666 1.00 48.75
7
ATOM 3374 CA GLN B 212 16.566 56.525 72.181 1.00 47.49
6
ATOM 3375 CB GLN B 212 16.712 56.943 73.643 1.00 74.54
6
ATOM 3376 CG GLN B 212 15.407 57.083 74.394 1.00 76.74
6
ATOM 3377 CD GLN B 212 15.620 57.235 75.889 1.00 78.20
6
ATOM 3378 OE1 GLN B 212 16.241 56.384 76.535 1.00 79.21
8
ATOM 3379 NE2 GLN B 212 15.099 58.318 76.450 1.00 78.87
7
ATOM 3380 C GLN B 212 16.011 57.678 71.342 1.00 46.38
6
ATOM 3381 O GLN B 212 14.860 57.645 70.908 1.00 46.06
8
ATOM 3382 N ALA B 213 16.840 58.690 71.104 1.00 58.18
7
ATOM 3383 CA ALA B 213 16.434 59.836 70.304 1:00 56.70
6
ATOM 3384 CB ALA B 213 17.506 60.900 70.344 1.00101.95
6
ATOM 3385 C ALA B 223 16.183 59.399 68.870 1.00 56.54
6
ATOM 3386 0 ALA B 213 15.253 59.886 68.229 1.00 56.71
8
ATOM 3387 N VAL B 214 17.020 58.478 68.380 1.00 57.27
7
ATOM 3388 CA VAL B 214 16.911 57.937 67.015 1.00 55.99
6
ATOM 3389 CB VAL B 214 17.914 56.784 66.758 1.00 38.97
6
ATOM 3390 CG1 VAL B 214 17.831 56.354 65.291 1.00 40.50
6
ATOM 3391 CG2 VAL B 214 19.336 57.210 67.130 1.00 39.20
6
ATOM 3392 C VAL B 214 15.519 57.359 66.847 1.00 55.21
6
ATOM 3393 O VAL B 214 14.976 57.258 65.748 1.00 54.40
8
ATOM 3394 N ALA B 215 14.963 56.955 67.972 1.00 35.62
7
ATOM 3395 CA ALA B 215 13.641 56.403 68.008 1.00 34.92
6
ATOM 3396 CB ALA B 215 13.494 55.553 69.237 1.00 13.87
6
ATOM 3397. C ALA B 215 12.692 57.581 68.064 1.00 34.79
6
ATOM 3398 0 ALA B 215 11.673 57.605 67.394 1.00 35.53
8
ATOM 3399 N ILE B 216 13.051 58.568 68.873 1.00 37.58
7
ATOM 3400 CA ILE B 216 12.229 59.760 69.043 1.00 38.65
6
ATOM 3402 CB ILE B 216 12.945 60.781 69.947 1.00 58.19
6
ATOM 3402 CG2 ILE B 216 11.991 61.919 70.298 1.00 59.13
6
ATOM 3403 CG1 ILE B 216 13.441 60.079 71.212 1.00 57.58
6
ATOM 3404 CD1 ILE B 216 14.218 60.959 72.142 1.00 58.61
6
ATOM 3405 C ILE B 216 11.891 60.414 67.699 1.00 39.48
6
ATOM 3406 0 ILE B 216 10.762 60.881 67.482 1.00 38.93
8
ATOM 3407 N LEU B 217 12.877 60.446 66.806 1.00 55.28
7
61

CA 02440409 2003-09-09
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ATOM 3408 CA LEU B 217 12.700 61.025 65.481 1.00 56.43
ATOM 3409 CB LEU B 217 14.066 61.305 64.855 1.00 34.79
ATOM 3410 CG LEU B 217 14.067 61.729 63.387 1.00 34.72
ATOM 3411 CD1 LEU B 217 13.484 63.118 63.280 1.00 33.77
ATOM 3412 CD2 LEU B 217 15.478 61.688 62.826 1.00 33.56
ATOM 3413 C LEU B 217 11.915 60.041 64.606 1.00 58.48
ATOM 3414 0 LEU B 217 10.955 60.420 63.931 1.00 58.68
ATOM 3415 N LYS B 218 12.340 58.775 64.635 1.00 79.73
ATOM 3416 CA LYS B 218 11.715 57.689 63.875 1.00 80.04
ATOM 3417 CB LYS B 218 12.239 56.347 64.384 1.00 60.98
ATOM 3418 CG LYS B 218 13.069 55.534 63.420 2.00 61.97
ATOM 3419 CD LYS B 218 13.516 54.221 64.104-1.00 64.17
'
ATOM 342 CE LYS B 218 14.311 53.257 63.198 1.00 65.47
0
ATOM 3421 NZ LYS B 218 14.927 52.116 63.964 1.00 65.03
ATOM 3422 C LYS B 218 10.191 57.722 64.026 1.00 81.14
ATOM 3423 0 LYS B 218 9.468 57.840 63.040 1.00 81.66
ATOM 3424 N GLU B 219 9.720 57.609 65.268 1.00 75.84
ATOM 3425 CA GLU B 219 8.295 57.626 65.579 1.00 77.32
ATOM 3426 CB GLU B 219 8.078 57.631 67.092 1.00140.14
ATOM 3427 CG GLU B 219 6.668 58.061 67.478 1.00143.68
ATOM 3428 CD GLU B 219 6.462 58.174 68.971 1.00145.94
ATOM 3429 OE1 GLU B 219 7.328 58.769 69.650 1.00146.59
ATOM 3430 OE2 GLU B 219 5.423 57.679 69.462 1.00147.42
ATOM 3431 C GLU B 219 7.556 58.819 64.977 1.00 78.30
ATOM 3432 O GLU B 219 6.477 58.659 64.396 1.00 78.84
ATOM 3433 N HIS B 220 8.121 60.014 65.138 1.00 66.20
ATOM 3434 CA HIS B 220 7.504 61.227 64.609 1.00 66.15
ATOM 3435 CB HIS B 220 8.338 62.448 64.994 1.00105.59
ATOM 3436 CG HIS B 220 8.015 62.991 66.349 1.00108.40
ATOM 3437 CD2 HIS B 220 8.405 62.596 67.584 1.00109.60
ATOM 3438 ND1 HIS B 220 7.157 64.054 66.540 1.00109.83
ATOM 3439 CE1 HIS B 220 7.034 64.291 67.834 1.00110.63
ATOM 3440 NE2 HIS B 220 7.782 63.420 68.489 1.00111.13
ATOM 3441 C HIS B 220 7.343 61.148 63.102 1.00 65.62
ATOM 3442 0 HIS B 220 6.311 61.534 62.561 1.00 65.51
ATOM 3443 N LEU B 221 ~ 8.370 60.640 62.429 1.00 64.25
ATOM 3444 CA LEU B 221 8.337 60.490 60.983 1.00 62.89
ATOM 3445 CB LEU B 221 9.520 59.655 60.508 1.00 40.10
ATOM 3446 CG LEU B 221 10.733 60.492 60.127 1.00 39.46
ATOM 3447 CD1 LEU B 221 12.014 59.665 60.231 1.00 38.63
ATOM 3448 CD2 LEU B 221 10.499 61.061 58.725 1.00 40.35
ATOM 3449 C LEU B 221 7.057 59.800 60.598 1.00 62.98
ATOM 3450 O LEU B 221 6.352 60.252 59.706 1.00 63.03
ATOM 3451 N ASN B 222 6.767 58.698 61.281 1.00 80.11
ATOM 3452 CA ASN B 222 5.561 57.927 61.033 1.00 80.71
ATOM 3453 CB ASN B 222 5.176 57.136 62.284 1.00106.39
ATOM 3454 CG ASN B 222 6.127 55.993 62.568 1.00108.39
ATOM 3455 OD1 ASN B 222 6.008 55.318 63.592 1.00109.32
ATOM 3456 ND2 ASN B 222 7.072 55.761 61.660 1.00108.36
ATOM 3457 C ASN B 222 4.411 58.842 60.642 1.00 80.66
ATOM 3458 0 ASN B 222 3.870 58.734 59.542 1.00 81.98
ATOM 3459 N TYR B 223 4.057 59.744 61.555 1.00 63.38
ATOM 3460 CA TYR B 223 2.974 60.706 61.371 1.00 63.46
ATOM 3461 CB TYR B 223 3.332 61.991 62.111 1.00 60.86
ATOM 3462 CG TYR B 223 3.569 61.736 63.585 1.00 60.10
ATOM 3463 CD1 TYR B 223 4.374 62.577 64.344 1.00 60.05
62

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3464 CE1 B 223 4.605 62.326 65.696 1.00 59.49 6
TYR
ATOM 3465 CD2 TYR B 223 2.993 60.637 64.217 1.00 60.08 6
ATOM 3466 CE2 'TYRB 223 3.214 60.379 65.560 1.00 60.21 6
ATOM 3467 CZ TYR B 223 4.022 61.224 66.297 1.00 59.74 6
ATOM 3468 OH TYR B 223 4.248 60.951 67.631 1.00 59.35 8
ATOM 3469 C TYR B 223 2.646 60.987 59.913 1.00 64.43 6
ATOM 3470 0 TYR B 223 1.480 61.164 59.558 1.00 64.54 8
ATOM 3471 N PHE B 224 3.678 61.022 59.074 1.00 73.75 7
ATOM 3472 CA PHE B 224 3.502 61.238 57.645 1.00 74.89 6
ATOM 3473 CB PHE B 224 4.815 61.699 56.983 1.00 86.72 6
ATOM 3474 CG PHE B 224 5.376 62.988 57.543 1.00 87.15 6
ATOM 3475 CD1 PHE B 224 ' 6.108 62.992 58.728 1.00 87.88 6
ATOM 3476 CD2 PHE B 224 5.171 64.197 56.885 1.00 86.49 6
ATOM 3477 CE1 PHE B 224 6.624 64.174 59.245 1.00 87.12 6
ATOM 3478 CE2 PHE B 224 5.684 65.385 57.398 1.00 85.69 6
ATOM 3479 CZ PHE B 224 6.410 65.373 58.578 1.00 86.13 6
ATOM 3480 C PHE B 224 3.061 59.897 57.053 1.00 76.36 6
ATOM 3481 0 PHE B 224 3.744 59.311 56.211 1.00 76.67 8
ATOM 3482 N ALA B 225 1.916 59.413 57.523 1.00 80.05 7
ATOM 3483 CA ALA B 225 1.360 58.149 57.059 1.00 81.59 6
ATOM 3484 CB ALA B 225 1.234 57.174 58.225 1.00 74.53 6
ATOM 3485 C ALA B 225 -0.001 58.365 56.407 1.00 83.07 6
ATOM 3486 0 ALA B 225 -0.103 58.491 55.188 1.00 83.49 8
ATOM 3487 N ASN B 226 -1.049 58.410 57.220 1.00 69.91 7
ATOM 3488 CA ASN B 226 -2.390 58.608 56.689 1.00 72.09 6
ATOM 3489 CB ASN B 226 -3.405 57.834 57.525 1.00 91.37 6
ATOM 3490 CG ASN B 226 -3.139 56.343 57.519 1.00 92.71 6
ATOM 3491 OD1 ASN B 226 -3.209 55.690 56.476 1.00 91.90 8
ATOM 3492 ND2 ASN B 226 -2.828 55.796 58.687 1.00 93.91 7
ATOM 3493 C ASN B 226 -2.734 60.088 56.670 1.00 73.36 6
ATOM 3494 O ASN B 226 -2.610 60.771 57.683 1.00 74.07 8
ATOM 3495 N PRO B 227 -3.157 60.602 55.502 1.00115.46 7
ATOM 3496 CD PRO B 227 -2.811 59.980 54.207 1.00 39.04 6
ATOM 3497 CA PRO B 227 -3.522 62.013 55.317 1.00116.36 6
ATOM 3498 CB PRO B 227 -2.727 62.390 54.077 1.00 39.80 6
ATOM 3499 CG PRO B 227 -2.919 61.146 53.223 1.00 39.44 6
ATOM 3500 C PRO B 227 -5.008 62.361 55.141 1.00118.18 6
ATOM 3501 O PRO B 227 -5.859 61.494 54.963 1.00118.42 8
ATOM 3502 N GLU B 228 -5.294 63.656 55.199 1.00106.47 7
ATOM 3503 CA GLU B 228 -6.636 64.181 54.991 1.00108.65 6
ATOM 3504 CB GLU B 228 -7.302 64.555 56.319 2.00273.49 6
ATOM 3505 CG GLU B 228 -8.736 65.043 56.146 1.00177.01 6
ATOM 3506 CD GLU B 228 -9.474 65.203 57.459 1.00179.13 6
ATOM 3507 OE1 GLU B 228 -9.021 65.999 58.309 1.00181.08 8
ATOM 3508 OE2 GLU B 228 -10.514 64.534 57.635 1.00180.01 8
ATOM 3509 C GLU B 228 -6.406 65.425 54.12$ 1.00110.13 6
ATOM 3510 0 GLU B 228 -6.091 66.501 54.635 1.00110.94 8
ATOM 3511 N ALA B 229 -6.561 65.265 52.818 1.00 60.59 7
ATOM 3512 CA ALA B 229 -6.311 66.344 51.876 1.00 61.24 6
ATOM 3513 CB ALA B 229 -5.424 65.825 50.764 1.00 14.58 6
ATOM 3514 C ALA B 229 -7.527 67.025 51.266 1.00 62.40 6
ATOM 3515 O ALA B 229 -8.670 66.677 51.551 1.00 62.49 8
ATOM 3516 N SER B 230 -7.236 68.005 50.413 1.00 80.99 7
ATOM 3517 CA SER B 230 -8.231 68.789 49.690 1.00 83.43 6
ATOM 3518 CB SER B 230 -8.273 70.220 50.237 1.00134.32 6
ATOM 3519 OG SER B 230 -8.505 70.230 51.635 1.00135.82 8
63

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3520 C SER B 230 -7.766 68.805 48.234 1.00 84.59
ATOM 3521 O SER B 230 -6.569 68.843 47.973 1.00 84.28
ATOM 3522 N SER B 231 -8.703 68.782 47.291 1.00152.80
ATOM 3523 CA SER B 231 -8.360 68.774 45.867 1.00154.78
ATOM 3524 CB SER B 231 -9.544 68.232 45.056 1.00208.87
ATOM 3525 OG SER B 231 -10.734 68.948 45.338 1.00208.87
ATOM 3526 C SER B 231 -7.933 70.141 45.320 1.00155.53
ATOM 3527 O SER B 231 -8.412 70.526 44.231 1.00155.94
ATOM 3528 OXT SER B 231 -7.102 70:803 45.978 1.00208.87
ATOM 3529 CB ALA C 2 8.362 95.868 32.510 1.00125.97
ATOM 3530 C ALA C 2 10.376 97.139 31.725 1.00 93.91
ATOM 3531 O ALA C 2 11.120 96.421 32.394 1.00 93,90
ATOM 3532 N ALA C 2 8.926 95.898 30.107 1.00 94.38
ATOM 3533 CA ALA C 2 8.955. 96.695 31.369 1.00 94.22
ATOM 3534 N ILE C 3 10.744 98.330 31.276 1.00119.26
ATOM 3535 CA ILE C 3 12.067 98.868 31.547 1.00117.83
ATOM 3536 CB ILE C 3 12.862 99.056 30.224 1.00 58.24
ATOM 3537 CG2 ILE C 3 14.219 99.663 30.496 1.00 58.03
ATOM 3538 CG1 ILE C 3 13.034 97.707 29.519 1.00 58.36
ATOM 3539 CD1 ILE C 3 14.019 96.765 30.177 1.00 56.18
ATOM 3540 C ILE C 3 11.892 100.21532.237 1.00117.16
ATOM 3541 0 ILE C 3 11.383 101.15531.628 1.00117,61
ATOM 3542 N LYS C 4 12.290 100.30733.507 1.00 25.32
ATOM 3543 CA LYS C 4 12.161 101.56534.243 1.00 24.08
ATOM 3544 CB LYS C 4 11.855 101.28735.718 1.00108.61
ATOM 3545 CG LYS C 4 11.397 102.52236.477 1.00111.82
ATOM 3546 CD LYS C 4 10.806 102.18837.843 1.00114,29
ATOM 3547 CE LYS C 4 10.245 103.44438.512 1.00116,05
ATOM 3548 NZ LYS C 4 9.624 103.18539.844 1.00117,65
ATOM 3549 C LYS C 4 13.417 102.44034.102 1.00 22.74
ATOM 3550 O LYS C 4 14.514 102.05434.485 1.00 21.59
ATOM 3551 N ARG C 5 13.258 103.62233.527 1.00 40,10
ATOM 3552 CA ARG C 5 14.405 104.48233.356 1.00 41.90
ATOM 3553 CB ARG C 5 14.312 105.22532.019 1.00 84.10
ATOM 3554 CG ARG C 5 15.613 105.92231.616 1.00 86.67
ATOM 3555 CD ARG C 5 15.822 105.90730.105 1.00 88.03
ATOM 3556 NE ARG C 5 17.166 106.34729.739 1.00 89.24
ATOM 3557 CZ ARG C 5 17.695 106.20628.527 1.00 90.34
ATOM 3558 NH1 ARG C 5 16.996 105.63527.553 1.00 91.45
ATOM 3559 NH2 ARG C 5 18.927 106.63328.286 1.00 90.51
ATOM 3560 C ARG C 5 14.526 105.46234.515 1.00 42.57
ATOM 3561 O ARG C 5 13.551 105.75435.198 1.00 42.05
ATOM 3562 N PHE C 6 15.743 105.94634.739 1.00 41.22
ATOM 3563 CA PHE C 6 16.053 106.89535.804 1.00 42.96
ATOM 3564 CB PHE C 6 16.862 106.19336.915 1.00 74.13
ATOM 3565 CG PHE C 6 16.116 105.09037.642 1.00 74.47
ATOM 3566 CD1 PHE C 6 15.633 103.98136.963 1.00 74.97
ATOM 3567 CD2 PHE C 6 15.898 105.17139.013 1.00 74.02
ATOM 3568 CE1 PHE C 6 14.942 102.97537.643 1.00 74.17
ATOM 3569 CE2 PHE C 6 15.210 104.17239.694 1.00 72.21
ATOM 3570 CZ PHE C 6 14.732 103.07639.010 1.00 72.32
ATOM 3571 C PHE C 6 26.950 107.91935.121 1.00 44.27
ATOM 3572 0 PHE C 6 17.774 107.50834.310 1.00 44.21
ATOM 3573 N GLY C 7 16.817 109.22135.408 1.00 93.24
ATOM 3574 CA GLY C 7 17.711 110.17734.750 1.00 95.08
ATOM 3575 C GLY C 7 17.441 111.68234.660 1.00 95.99
64

CA 02440409 2003-09-09
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ATOM 3576 0 GLY C 7 16.818 112.268 35.548 1.00 95.32
ATOM 3577 N ARG C 8 17.956 112.298 33.583 1.00 71.19
ATOM 3578 CA ARG C 8. 17.813 113.738 33.283 1.00 72.07
ATOM 3579 CB ARG C 8 18.364 114.580 34.432 1.00113.66
ATOM 3580 CG ARG C 8 17.325 115.062 35.414 1.00115.61
ATOM 3581 CD ARG C 8 17.948 116.047 36.382 1.00118.64
ATOM 3582 NE ARG C 8 16.997 116.518 37.381 1.00121.32
ATOM 3583 CZ ARG C 8 17.309 117.340 38.377 1.00122.59
ATOM 3584 NH1 ARG C 8 18.553 117.786 38.506 1.00121.72
ATOM 3585 NH2 ARG C 8 16.377 117.713 39.245 1.00124.20
ATOM 3586 C ARG C 8 18.469 114.214 31.958 1.00 71.82
ATOM 3587 O ARG C 8 19.507 113.707 31.543 1.00 71.24
ATOM 3588 N ILE C 9 17.844 115.204 31.319 1.00 97.66
ATOM 3589 CA ILE C 9 18.289 115.817 30.055 1.00 99.18
ATOM 3590 CB ILE C 9 19.471 116.816 30.286 1.00113.15
ATOM 3591 CG2 ILE C 9 19.860 117.489 28.975 1.00113.13
ATOM 3592 CG1 ILE C 9 19.043 117.954 31.221 1.00113.50
ATOM 3593 CD1 ILE C 9 18.806 117.547 32.651 1.00114.11
ATOM 3594 C ILE C 9 18.652 114.882 28.889 1.00 99.69
ATOM 3595 0 ILE C 9 17.787 114.176 28.371 1.00 99.98
ATOM 3596 N ARG C 10 19.923 114.907 28.479 1.00 91.44
ATOM 3597 CA ARG C 10 20.469 114.115 27.365 1.00 91.76
ATOM 3598 CB ARG C 10 19.799 112.738 27.277 1.00175.32
ATOM 3599 CG ARG C 10 20.491 121.780 26.317 1.00178.69
ATOM 3600 CD ARG C 10 19.809 110.423 26.287 1.00181.18
ATOM 3601 NE ARG C 10 20.567 109.457 25.498 1.00183.82
ATOM 3602 CZ ARG C 10 20.194 108.196 25.303 1.00185.19
ATOM 3603 NH1 ARG C 10 19.067 107.745 25.838 1.00185.55
ATOM 3604 NH2 ARG C 10 20.950 107.385 24.575 1.00186.22
ATOM 3605 C ARG C 10 20.281 114.875 26.045 1.00 91.37
ATOM 3606 0 ARG C 10 19.738 114.343 25.074 1.00 91.78
ATOM 3607 N GLU C 11 20.758 116.119 26.028 1.00 37.16
~
ATOM 3608 CA GLU C 11 20.654 117.028 24.883 1.00 36.54
ATOM 3609 CB GLU C 11 21.407 118.309 25.202 1.00 82.90
ATOM 3610 CG GLU C 11 21.511 119.279 24.057 1.00 84.74
ATOM 3611 CD GLU C 11. 22.173 120.560 24.489 1.00 86.15
ATOM 3612 OE1 GLU C 11 23.250 120.472 25.108 1.00 87.27
ATOM 3613 OE2 GLU C 11 21.624 121.651 24.221 1.00 87.25
ATOM 3614 C GLU C 11 21.087 116.547 23.509 1.00 36.03
ATOM 3615 0 GLU C 11 20.621 117.077 22.505 1.00 36.52
ATOM 3616 N VAL C 12 21.980 115.563 23.453 1.00 63.96
ATOM 3617 CA VAL C 12 22.465 115.037 22.174 1.00 63.48
ATOM 3618 CB VAL C 12 23.871 115.645 21.828 1.00 43.42
ATOM 3619 CG1 VAL C 12 24.359 115.158 20.481 1.00 43.03
ATOM 3620 CG2 VAL C 12 23.782 117.160 21.807 1.00 41.74
ATOM 3621 C VAL C 12 22.525 113.498 22.181 1.00 63.52
ATOM 3622 0 VAL C 12 21.596 112.838 22.653 1.00 64.01
ATOM 3623 N ILE C 13 23.614 112.945 21.651 1.0'0 82.06
ATOM 3624 CA ILE C 13 23.830 111.502 21.563 1.00 82.83
ATOM 3625 CB ILE C 13 23.440 110.967 20.150 1.00 46.79
ATOM 3626 CG2 ILE C 13 24.586 110.181 19.525 1.00 46.26
ATOM 3627 CG1 ILE C 13 22.197 110.086 20.244 1.00 46.68
ATOM 3628 CD1 ILE C 13 20.964 110.830 20.703 1.00 47.84
ATOM 3629 C ILE C 13 25.313 111.220 21.813 1.00 83.68
ATOM 3630 0 ILE C 13 26.152 112.109 21.632 1.00 84.19
ATOM 3631 N PRO C 14 25.658 109.979 22.226 1.00200.26

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3632 CD PRO C 14 24.784 108.80422.390 1.00143.05
ATOM 3633 CA PRO C 14 27.055 109.61322.492 1.00200.29
ATOM 3634 CB PRO C 14 26.958 108.14422.909 1.00142.05
ATOM 3635 CG PRO C 14 25.752 107.66622.176 1.00143.08
ATOM 3636 C PRO C 14 27.968 109.82721.289 1.00198.70
ATOM 3637 O PRO C 14 29.189 109.73521.406 1.00199.16
ATOM 3638 N LEU C 15 27.362 110.10420.137 1.00 90.84
ATOM 3639 CA LEU C 15 28.091 110.36918.901 1.00 91.40
ATOM 3640 CB LEU C 15 29.307 111.24219.178 1.00 74.19
ATOM 3641 CG LEU C 15 30.246 111.44817.993 1.00 73.88
ATOM 3642 CD1 LEU C 15 29.550 112.19316.853 1.00 73.64
ATOM 3643 CD2 LEU C 15 31.437 112.21818.495 1.00 73.28
ATOM 3644 C LEU C 15 28.551 109.14618.135 1.00 91.83
ATOM 3645 0 LEU C 15 29.665 108.65918.333 1.00 92.24
ATOM 3646 N PRO C 16 27.711 108.65317.216 1.00 77.46
ATOM 3647 CD PRO C 16 26.444 109.24316.749 1.00201.81
ATOM 3648 CA PRO C 16 28.062 107.47516.421 1.00 76.68
ATOM 3649 CB PRO C 16 26.816 107.25515.567 1.00201.48
ATOM 3650 CG PRO C 16 26.309 108.65115.365 1.00202.73
ATOM 3651 C PRO C 16 29.312 107.70315.579 1.00 74.58
ATOM 3652 O PRO C 16 29.660 108.83115.248 1.00 73.75
ATOM 3653 N PRO C 17 29.999 106.62215.222 1.00 94.18
ATOM 3654 CD PRO C 17 30.910 106.52914.067 1.00208.87
ATOM 3655 CA PRO C 17 29.555 105.29415.633 1.00 92.59
ATOM 3656 CB PRO C 17 29.755 104.47914.370 1.00208.87
ATOM 3657 CG PRO C 17 31.069 105.02213.882 1.00208.87
ATOM 3658 C PRO C 17 30.410 104.77816.767 1.00 88.70
ATOM 3659 O PRO C 17 30.400 103.58817.055 1.00 88.26
ATOM 3660 N LEU C 18 31.143 105.67617.410 1.00 55.26
ATOM 3661 CA LEU C 18 32.039 105.28118.485 1.00 51.42
ATOM 3662 CB LEU C 18 31.421 104.18419.356 1.00 53.94
ATOM 3663 CG LEU C 18 30.219 104.51820.241 1.00 53.68
ATOM 3664 CD1 LEU C 18 29.655 103.21920.819 1.00 53.23
ATOM 3665 CD2 LEU C 18 30.643 105.47521.358 1.00 52.67
ATOM 3666 C LEU C 18 33.291 104.75217.804 1.00 49.36
ATOM 3667 0 LEU C 18 34.399 105.08718.201 1.00 49.27
ATOM 3668 N THR C 19 33.109 103.92016.780 1.00 20.15
ATOM 3669 CA THR C 19 34.236 103.38716.017 1.00 18.27
ATOM 3670 CB THR C 19 33.908 102.07015.315 1.00 40.27
ATOM 3671 OG1 THR C 19 32.747 102.24814.494 1.00 40.82
ATOM 3672 CG2 THR C 19 33.669 100.97416.318 1.00 40.29
ATOM 3673 C THR C 19 34.504 104.41114.925 1.00 17.52
ATOM 3674 O THR C 19 34.690 104.06313.759 1.00 16.43
ATOM 3675 N GLU C 20 34.503 105.67815.314 1.00 36.78
ATOM 3676 CA GLU C 20 34.730 106.75914.378 1.00 38.00
ATOM 3677 CB GLU C 20 33.761 107.90014.677 1.00 83.62
ATOM 3678 CG GLU C 20 33.772 109.03313.669 1.00 85.64
ATOM 3679 CD GLU C 20 33.406 110.35714.311 1.00 87.68
ATOM 3680 OE1 GLU C 20 33.203 111.35113.579 1.00 88.74
ATOM 3681 OE2 GLU C 20 33.335 110.40115.560 1.00 88.07
..ATOM3682 C GLU C 20 36.171 107.25614.458 1.00 37.77
ATOM 3683 0 GLU C 20 36.635 107.95213.547 1.00 37.06
ATOM 3684 N ILE C 21 36.882 106.91715.537 1.00 33.12
ATOM 3685 CA ILE C 21 38.271 107.36115.648 1.00 35.17
ATOM 3686 CB ILE C 21 39.008 106.78216.864 1.00 70.71
ATOM 3687 CG2 ILE C 21 40.388 107.42116.972 1.00 70.79
66

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3688 CG1 ILE C 21 38.240 107.095 18.1441.00 72.41
6
ATOM 3689 CD1 ILE C 22 38.219 108.584 18.4281.00 73.92
6
ATOM 3690 C ILE C 21 38.911 106.808 14.4021.00 36.07
6
ATOM 3691 O ILE C 21 39.725 107.473 13.7661.00 35.93
8
ATOM 3692 N GLN C 22 38.513 105.580 14.0671.00 69.90
7
ATOM 3693 CA GLN C 22 38.978 104.882 12.8741.00 71.08'
6
ATOM 3694 CB GLN C 22 39.300 103.429 13.2061.00 51.83
6
ATOM 3695 CG GLN C 22 40.499 103.265 14.1241.00 51.01
6
ATOM 3696 CD GLN C 22 .40.953 101.825 14.2321.00 50.49
6
ATOM 3697 OE1 GLN C 22 40.187 100.959 14.6511.00 51.17
8
ATOM 3698 NE2 GLN C 22 42.202 101.561 13.8541.00 49.03
7
ATOM 3699 C GLN C 22 37.827 104.958 11.8801.00 72.24
6
ATOM 3700 O GLN C 22 37.053 105.910 11.9261.00 72.58
8
ATOM 3701 N VAL C 23 37.698 103.979 10.9891.00 54.37
7
ATOM 3702 CA VAL C 23 36.595 104.006 10.0311.00 55.27
6
ATOM 3703 CB VAL C 23 35.227 103.880 10.7461.00 45.46
6
ATOM 3704 CG1 VAL C 23 34.080 104.090 9.748 1.00 45.92
6
ATOM 3705 CG2 VAL C 23 35.117 102.528 11.4231.00 46.45
6
ATOM 3706 C VAL C 23 36.588 105.323 9.270 1.00 56.22
6
ATOM 3707 0 VAL C 23 37.149 105.423 8.180 1.00 57.00
8
ATOM 3708 N GLU C 24 35.933 106.324 9.862 1.00 39.91
7
ATOM 3709 CA GLU C 24 35.821 107.663 9.288 1.00 41.34
6
ATOM 3710 CB GLU C 24 34.757 108.486 10.0251.00111.93
6
ATOM 3711 CG GLU C 24 33.323 108.053 9.752 1.00116.16
6
ATOM 3712 CD GLU C 24 32.307 108.949 10.4301.00118.56
6
ATOM 3713 OE1 GLU C 24 32.302 110.163 10.1481.00120.21
8
ATOM 3714 OE2 GLU C 24 31.507 108.446 11.2451.00120.53
8
ATOM 3715 C GLU C 24 37.150 108.376 9.365 1.00 40.87
6
ATOM 3716 O GLU C 24 37.227 109.589 9.188 1.00 41.48
8
ATOM 3717 N SER C 25 38.194 107.609 9.643 1.00 48.64
7
ATOM 3718 CA SER C 25 39.533 108.154 9.722 1.00 48.38
6
ATOM 3719 CB SER C 25 40.287 107.593 10.9301.00 73.51
6
ATOM 3720 OG SER C 25 41.621 108.083 10.9831.00 73.78
8
ATOM 3721 C SER C 25 40.255 107.750 8.461 1.00 47.83
6
ATOM 3722 O SER C 25 40.710 108.593 7.698 1.00 47.42
8
ATOM 3723 N TYR C 26 40.346 106.446 8.243 1.00 54.43
7
ATOM 3724 CA TYR C 26 41.034 105.924 7.077 1.00 54.68
6
ATOM 3725 CB TYR C 26 40.858 104.404 7.005 1.00 49.43
6
ATOM 3726 CG TYR C 26 42.038 103.677 6.406 1.00 47.66
6
ATOM 3727 CD1~TYR C 26 43.210 103.498 7.128 1.00 46.59
6
ATOM 3728 CE1 TYR C 26 44.299 102.857 6.572 ,1.00 46.24
6
ATOM 3729 CD2 TYR C 26 41.990 103.191 5.109 1.00 47.36
6
ATOM 3730 CE2 TYR C 26 43.077 102.550 4.549 1.00 47.16
6
ATOM 3731 CZ TYR C 26 44.227 102.384 5.282 1.00 46.35
6
ATOM 3732 OH TYR C 26 45.303 101.734 4.718 1.00 46.49
8
ATOM 3733 C TYR C 26 40.480 106.572 5.814 1.00 55.37
6
ATOM 3734 O TYR C 26 41.221 107.148 5.014 1.00 55.48
8
ATOM 3735 N LYS C 27 39.167 106.474 5.654 1.00 60.13
7
ATOM 3736 CA LYS C 27 38.474 107.031 4.503 1.00 60.39
6
ATOM 3737 CB LYS C 27 36.976 107.042 4.777 2.00 79.68
6
ATOM 3738 CG LYS C 27 36.140 107.634 3.670 1.00 81.33
6
ATOM 3739 CD LYS C 27 34.664 107.567 4.037 1.00 83.89
6
ATOM 3740 CE LYS C 27 33.765 108.050 2.909 1.00 85.68
6
ATOM 3741 NZ LYS C 27 32.338 107.749 3.215 1.00 86.18
7
ATOM 3742 C LYS C 27 38.947 108.442 4.188 1.00 60.15
6
ATOM 3743 O LYS C 27 39.368 108.729 3.070 1.00 60.17
8
67

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
~,
ATOM 3744 N LYS C 28 38.889 109.3175.188 1'.00 50.23
7
ATOM 3745 CA LYS C 28 39.283 110.7095.008 1.00 51.29
6
ATOM 3746 CB LYS C 28 38.838 111.5576.205 1.00208.87
6
ATOM 3747 CG LYS C 28 39.587 111.2767.499 1.00208.87
6
ATOM 3748 CD LYS C 28 39.211 112.278~ 8.581 1.00208.87
6
ATOM 3749 CE LYS C 28 40.027 112.0629.843 1.00208.87
6
ATOM 3750 NZ LYS C 28 39.705 113.07810.880 1.00208.87
7
ATOM 3751 C LYS C 28 40.776 110.8724.802 1.00 50.07
6
ATOM 3752 O LYS C 28 41.266 111.9914.625 1.00 49.02
8
ATOM 3753 N ALA C 29 41:491 109.7534.841 1.00 30.72
7
ATOM 3754 CA ALA C 29 42.934 109.7484.647 1.00 30.33
6
ATOM 3755 CB ALA C 29 43.617 108.9955.766 1.00 73.05
6
ATOM 3756 C ALA C 29 43.108 109.0153.349 1.00 30.18
6
ATOM 3757 0 ALA C 29 43.954 109.3632.528 1.00 29.71
8
ATOM 3758 N LEU C 30 42.282 107.9903.184 1.00 47.18
7
ATOM 3759 CA LEU C 30 42.264 107.1701.988 1.00 49.80
6
ATOM 3760 CB LEU C 30 41.920 105.7272.355 1.00 77.57
6
ATOM 3761 CG LEU C 30 41:923 104.7201.207 1.00 79.55
6
ATOM 3762 CD1 LEU C 30 43.315 104.5990.633 1.00 80.89
6
ATOM 3763 CD2 LEU C 30 41.438 103.3841.708 1.00 79.00
6
ATOM 3764 C LEU C 30 41.190 107.7701.067 1.00 50.91
6
ATOM 3765 O LEU C 30 40.442 107.0560.390 1.00 50.20
8
ATOM 3766 N GLN C 31 41.126 109.1001.071 1.00108.17
7
ATOM 3767 CA GLN C 31 40.181 109.8740.269 1.00108.50
6
ATOM 3768 CB GLN C 31 40.839 111.190-0.137 1.00 55.88
6
ATOM 3769 CG GLN C 31 40.877 112.1840.993 1.00 54.19
6
ATOM 3770 CD GLN C 31 41.888 113.2910.797 1.00 54.53
6
ATOM 3771 OE1 GLN C 31 42.243 113.639-0.321 1.00 53.64
8
ATOM 3772 NE2 GLN C 31 42.350 113.8641.900 1.00 55.77
7
ATOM 3773 C GLN C 31 39.666 109.141-0.963 1.00110.03
6
ATOM 3774 0 GLN C 31 38.491 109.245-1.312 1.00110.78
8
ATOM 3775 N ALA C 32 40.567 108.412-1.610 1.00112.39
7
ATOM 3776 CA ALA C 32 40.277 107.621-2.801 1.00114.14
6
ATOM 3777 CB ALA C 32 40.562 106.153-2.511 1.00162.72
6
ATOM 3778 C ALA C 32 38.888 107.755-3.409 1.00115.03
6
ATOM 3779 0 ALA C 32 38.744 108.151-4.563 1.00115.39
8
ATOM 3780 N ASP C 33 37.873 107.405-2.630 1.00 75.66
7
ATOM 3781 CA ASP C 33 36.489 107.449-3.075 1.00 76.40
6
ATOM 3782 CB ASP C 33 35.594 106.892-1.967 1.00 98.63
6
ATOM 3783 CG ASP C 33 34.129 106.919-2.333 1.00 99.96
6
ATOM 3784 OD1 ASP C 33 33.366 107.650-1.662 1.00100.46
8
ATOM 3785 OD2 ASP C 33 33.745 106.219-3.293 1.00100.94
8
ATOM 3786 C ASP C'33 35.943 108.802-3.546 1.00 76.86
6
ATOM 3787 O ASP C 33 34.732 108.996-3.594 1.00 77.28
8
ATOM 3788 N VAL C 34 36.819 109.738-3.898 1.00106.73
7
ATOM 3789 CA VAL C 34 36.361 111.039-4.392 1.00108.50
6
ATOM 3790 CB VAL C 34 36.564 112.136--3.340 1.00 44.84
6
ATOM 3791 CG1 VAL C 34 37.139 113.387-3.962 1.00 43.21
6
ATOM 3792 CG2 VAL C 34 35.234 112.468-2.722 1.00 46.76
6
ATOM 3793 C VAL C 34 37.035 111.450-5.702 1.00110.20
6
ATOM 3794 O VAL C 34 38.263 111.561-5.772 1.00111.68
8
ATOM 3795 N PRO C 35 36.229 111.701-6.754 1.00 66.15
7
ATOM 3796 CD PRO C 35 34.774 111.914-6.644 1.00152.93
6
ATOM 3797 CA PRO C 35 36.704 112.097-8.082 1.00 66.89
6
ATOM 3798 CB PRO C 35 35.556 112.940-8.609 1.00152.71
6
ATOM 3799 CG PRO C 35 34.376 112.198-8.084 1.00153.29
6
6~

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3800 C PRO C 35 38.005 112.865 -7.999 1.00 67.29
ATOM 3801 O PRO C 35 38.047 113.955 -7.444 2.00 68.02
ATOM 3802 N PRO C 36 39.090 112.284 -8.534 1.00 59.92
ATOM 3803 CD PRO C 36 39.041 110.962 -9.189 1.00 60.17
ATOM 3804 CA PRO C 36 40.452 112.821 -8.576 1.00 61.78
ATOM 3805 CB PRO C 36 41.056 112.088 -9.763 1.00 60.22
ATOM 3806 CG PRO C 36 40.506 110.707 -9.559 1.00 60.65
ATOM 3807 C PRO C 36 40.592 114.331 -8.683 1.00 62.61
ATOM 3808 0 PRO C 36 41.698 114.859 -8.596 1.00 63.80
ATOM 3809 N GLU C 37 39.476 115.023 -8.877 1.00 97.57
ATOM 3810 CA GLU C 37 39.491 116.475 -8.975 1.00 98.61
ATOM 3811 CB GLU C 37 38.189 116.969 -9.615 1.00120.72
ATOM 3812 CG GLU C 37 37.946 116.409 -11.018 1.00122.45
ATOM 3813 CD GLU C 37 36.725 117.014 -11.695 1.00123.66
ATOM 3814 OE1 GLU C 37 36.713 118.245 -11.915 1.00124.43
ATOM 3815 OE2 GLU C 37 35.775 116.265 -12.011 1.00124.87
ATOM 3816 C GLU C 37 39.691 117.112 -7.588 1.00 98.50
ATOM 3817 0 GLU C 37 40.606 117.923 -7.401 1.00 98.69
ATOM 3818 N LYS C 38 38.856 116.740 -6.614 1.00 57.30
ATOM 3819 CA LYS C 38 38.989 117.296 -5.265 1.00 57.57
ATOM 3820 CB LYS C 3$ 37.606 117.569 -4.649 1.00126.96
ATOM 3821 CG LYS C 38 36.600 116.430 -4.700 1.00128.66
ATOM 3822 CD LYS C 38 35.284 116.855 -4.040 1.00129.19
ATOM 3823 CE LYS C 38 34.243 115.747 -4.058 1.00129.10
ATOM 3824 NZ LYS C 38 32.995 116.155 -3.356 1.00129.49
ATOM 3825 C LYS C 38 39.859 116.493 -4.284 1.00 57.41
ATOM 3826 0 LYS C 38 40.545 115.538 -4.665 1.00 55.85
ATOM 3827 N ARG C 39 39.819 116.895 -3.016 1.00 79.57
ATOM 3828 CA ARG C 39 40.634 116.273 -1.977 1.00 80.58
ATOM 3829 CB ARG C 39 40.572 114.748 -2.073 1.00131.15
ATOM 3830 CG ARG C 39 39.238 114.116 -1.677 1.00133.62
ATOM 3831 CD ARG C 39 38.881 114.351 -0.209 1.00136.29
ATOM 3832 NE ARG C 39 37.995 113.303 0.304 1.00139.40
ATOM 3833 CZ ARG C 39 37.444 123.300 2.516 1.00140.64
ATOM 3834 NH1 ARG C 39 37.675 114.296 2.363 1.00141.73
ATOM 3835 NH2 ARG C 39 36.664 112.294 1.889 1.00141.12
ATOM 3836 C ARG C 39 42.039 116.774 -2.289 1.00 80.41
ATOM 3837 0 ARG C 39 42.501 116.635 -3.422 1.00 80.59
ATOM 3838 N GLU C 40 42.714 117.368 -1.306 1.00133.71
ATOM 3839 CA GLU C 40 44.045 117.921 -1.554 1.00134.19
ATOM 3840 CB GLU C 40 44.014 119.450 -1.429 1.00153.39
ATOM 3841 CG GLU C 40 45.378 120.116 -1.608 1.00154.96
ATOM 3842 CD GLU C 40 45.289 121.626 -1.736 1.00155.83
ATOM 3843 OE1 GLU C 40 44.748 122.276 -0.820 1.00156.11
ATOM 3844 OE2 GLU C 40 45.765 122.162 -2.757 1.00156.29
ATOM 3845 C GLU C 40 45.210 117.396 -0.731 1.00133.67
ATOM 3846 0 GLU C 40 46.237 117.014 -1.292 1.00133.47
ATOM 3847 N ASN C 41 45.079 117.393 0.591 1.00 48.89
ATOM 3848 CA ASN C 41 46.184 116.913 1.410 1.00 48.90
ATOM 3849 CB ASN C 41 46.763 118.069 2.228 1.00130.74
ATOM 3850 CG ASN C 41 47.479 119.084 1.364 1.00132.51
ATOM 3851 OD1 ASN C 41 48.284 118.723 0.502 1.00133.26
ATOM 3852 ND2 ASN C 41 47.199 120.362 1.593 1.00133.67
ATOM 3853 C ASN C 41 45.946 115.713 2.328 1.00 47.57
ATOM 3854 0 ASN C 41 44.923 115.032 2.263 1.00 47.19
ATOM 3855 N VAL C 42 46.941 115.457 3.168 1.00113.68
69

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3856 CA VALC 42 46.908 114.361 4.123 1.00113.62
ATOM 3857 CB VALC 42 45.718 114.516 5.104 1.00120.00
ATOM 3858 CG1 VALC 42 45.857 113.533 6.264 1.00119.44
ATOM 3859 CG2 VALC 42 45.661 115.946 5.620 1.00120.00
ATOM 3860 C VAL.C 42 46.834 112.990 3.443 1.00113.04
ATOM 3861 O VALC 42 46.163 112.811 2.418 1.00112.64
ATOM 3862 N GLYC 43 47.554 112.037 4.028 1.00 97.34
ATOM 3863 CA GLYC 43 47.580 110.680 3.526 1.00 95.90
ATOM 3864 C GLYC 43 47.760 110.517 2.033 1.00 95.43
ATOM 3865 O GLYC 43 48.371 111.348 1.364 1.00 95.25
ATOM 3866 N~ ILEC 44 47.216 109.411 1.532 1.00 98.61
ATOM 3867 CA ILEC 44 47.267 109.030 0.126 1.00 97.86
ATOM 3868 CB ILEC 44 45.845 108.599 -0.360 1.00117.32
ATOM 3869 CG2 ILEC 44 45.725 108.710 -1.883 1.00118.51
ATOM 3870 CG1 ILEC 44 45.567 107.156 0.076 1.00117.91
ATOM 3871 CD1 ILEC 44 45.689 106.909 1.559 1.00119.46
ATOM 3872 C ILEC 44 47.842 110.086 -0.815 1.00 96.77
ATOM 3873 O ILEC 44 48.942 109.927 -1.344 1.00 96.62
ATOM 3874 N GLNC 45 47.098 111.168 -1.001 1.00 64.52
ATOM 3875 CA GLNC 45 47.503 112.235 -1.899 1.00 63.24
ATOM 3876 CB GLNC 45 46.306 113.144 -2.178 1.00134.09
ATOM 3877 CG GLNC 45 46.562 114.199 -3.2.261.00134.29
ATOM 3878 CD GLNC 45 45.286 114.852 -3.692 1.00135.13
ATOM 3879 OE1 GLNC 45 44.484 115.316 -2.883 1.00136.75
ATOM 3880 NE2 GLNC 45 45.090 114.896 -5.003 1.00135.00
ATOM 3881 C GLNC 45 48.677 113.053 -1.386 1.00 61.15
ATOM 3882 O GLNC 45 49.646 113.280 -2.110 1.00 60.47
ATOM 3883 N ALAC 46 48.585 113.510 -0.143 1.00 62.10
ATOM 3884 CA ALAC 46 49.661 114.294 0.445 1.00 60.83
ATOM 3885 CB ALAC 46 49.509 114.356 1.955 1.00131.32
ATOM 3886 C ALAC 46 50.954 113.594 0.080 1.00 59.10
ATOM 3887 O ALAC 46 51.696 114.060 -0.776 1,00 58.97
ATOM 3888 N ALAC 47 51.191 112.452 0.716 1,00 76.48
ATOM 3889 CA ALAC 47 52.387 111.657 0.467 1.00 75.00
ATOM 3890 CB ALAC 47 52.158 110.221 0.947 1.00 76.20
ATOM 3891 C ALAC 47 52.780 111.674 -1,019 1.00 74.00
ATOM 3892 O ALAC 47 53.961 111.600 -1.368 1.00 74.34
ATOM 3893 N PHEC 48 51.787 111.767 -1.895 1.00 40.16
ATOM 3894 CA PHEC 48 52.065 111.811 -3.313 1.00 38.41
ATOM 3895 CB PHEC 48 50.795 121.553 -4.122 1.00 63.46
ATOM 3896 CG PHEC 48 50.569 110.105 -4.456 1.00 63.24
ATOM 3$97 CD1 PHEC 48 51.206 109.099 -3.735 1.00 62.54
ATOM 3898 CD2 PHEC 48 49.701 109.747 -5.481 1.00 63.01
ATOM 3899 CE1 PHEC 48 50.983 107.766 -4.031 1.00 61.74
ATOM 3900 CE2 PHEC 48 49.470 108.414 -5.780 1.00 61.69
ATOM 3901 CZ PHEC 48 50.111 107.422 -5.056 1.00 61.01
ATOM 3902 C PHEC 48 52.612 113.185 -3.618 1.00 38.13
ATOM 3903 O PHEC 48 53.794 113.320 -3.942 1.00 39.04
ATOM 3904 N LYSC 49 51.756 114.202 -3.500 1.00 31.04
ATOM 3905 CA LYSC 49 52.164 115.574 -3.760 1.00 29.55
ATOM 3906 CB LYSC 49 51.042 116.541 -3.420 1.00 43.45
ATOM 3907 CG LYSC 49 49.942 116.566 -4.457 1.00 43.78
ATOM 3908 CD LYSC 49 48.890 117.607 -4.106 1.00 44.92
ATOM 3909 CE LYSC 49 47.993 117.952 -5.295 1.00 45.14
ATOM 3910 NZ LYSC 49 47.157 119.174 -5.031 1.00 45.76
ATOM 3911 C LYSC 49 53.383 115.848 -2.912 1.00 29.35

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 3912 0 LYS C 49 54.371 116.390 -3.391 1.00 28.88
ATOM 3923 N GLU C 50 53.325 115.451 -1.652 1.00 18.42
ATOM 3914 CA GLU C 50 54.461 115.636 -0.76 1.00 20.92
8
ATOM 3915 CB GLU C 50 54.285 114.825 0.515 1.00124.61
ATOM 3916 CG GLU C 50 53.448 115.494 1.583 1.00127.58
ATOM 3917 CD GLU C 50 53.419 114.679 2.851 1.00129.37
ATOM 3918 OE1 GLU C 50 54.507 114.257 3.299 1.00128.96
ATOM 3919 OE2 GLU C 50 52.315 114.463 3.397 1.00132.46
ATOM 3920 C GLU C 50 55.743 115.194 -1.467 1.00 21.08
ATOM 3921 0 GLU C 50 56.631 116.000 -1.719 1.00 22.13
ATOM 3922 N THR C 51 55.852 113.912 -1.777 1.00 71.12
ATOM 3923 CA THR C 51 57.046 113.427 -2.442 1.00 72.37
ATOM 3924 CB THR C 51 56.886 111.980 -2.832 1.00 31.71
ATOM 3925 OG1 THR C 51 56.273 111.262 -1.759 1.00 30.28
ATOM 3926 CG2 THR C 51 58.230 111.386 -3.103 1.00 31.58
ATOM 3927 C THR C 51 57.249 114.262 -3.697 1.00 74.48
ATOM 3928 0 THR C 51 56.557 114.071 -4.701 1.00 74.18
ATOM 3929 N PHE C 52 58.194 115.195 -3.641 1.00207.23
ATOM 3930 CA PHE C 52 58.437 116.065 -4.781 1.00208.87
ATOM 3931 CB PHE C 52 59.544 117.102 -4.471 1.00106.21
ATOM 3932 CG PHE C 52 60.822 116.525 -3.893 1.00106.76
ATOM 3933 CD1 PHE C 52 61.419 115.387 -4.434 1.00106.34
ATOM 3934 CD2 PHE C 52 61.462 227.171 -2.836 1.00106.63
ATOM 3935 CE1 PHE C 52 62.635 114.907 -3.930 1.00106.88
ATOM 3936 CE2 PHE C 52 62.675 116.701 -2.327 1.00106.95
ATOM 3937 CZ PHE C 52 63.262 115.568 -2.874 1.00107.20
ATOM 3938 C PHE C 52 58.752 115.329 -6.079 1.00208.87
ATOM 3939 O PHE C 52 59.429 114.299 -6.075 1.00208.87
ATOM 3940 N PRO C 53 58.227 115.837 -7.209 1.00122.42
ATOM 3941 CD PRO C 53 57.338 117.005 -7.345 1.00 39.07
ATOM 3942 CA PRO C S3 58.467 225.224 -8.514 2.00122.58
ATOM 3943 CB PRO C 53 58.031 116.313 -9.478 1.00 38.23
ATOM 3944 CG PRO C 53 56.863 116.884 -8.778 1.00 38.12
ATOM 3945 C PRO C 53 59.947 114.921 -8.634 1.00123.24
ATOM 3946 0 PRO C 53 60.766 115.837 -8.679 1.00124.61
ATOM 3947 N ILE C 54 60.293 113.641 -8.676 1.00 59.25
ATOM 394 CA ILE C 54 61.691 113.260 -8.772 1.00 59.00
8
ATOM 3949 CB ILE C 54 61.854 111.761 -9.153 1.00 38.24
ATOM 3950 CG2 ILE C 54 60.999 111.441 -10.3611.00 38.42
ATOM 3951 CG1 ILE C 54 63.335 111.442 -9.418 1.00 38.13
ATOM 3952 CD1 ILE C 54 64.270 111.795 -8.272 1.00 35.57
ATOM 3953 C ILE C 54 62.472 114.127 -9.753 1.00 59.79
ATOM 3954 O ILE C S4 62.206 214.152 -10.9551.00 59.16
ATOM 3955 N GLU C 55 63.420 114.871 -9.199 1.00 61.34
ATOM 3956 CA GLU C 55 64.285 115.720 -9.988 1.00 63.19
ATOM 3957 CB GLU C 55 65.047 116.698 -9.085 1.00 88.10
ATOM 3958 CG GLU C 55 64.181 117.597 -8.191 1.00 89.00
ATOM 3959 CD GLU C 55 63.485 116.848 -7.068 1.00 89.60
ATOM 3960 OE1 GLU C 55 64.139 116.016 -6.408 1.00 90.86
ATOM 3961 OE2 GLU C 55 62.288 117.106 -6.833 1.00 88.74
ATOM 3962 C GLU C 55 65.256 114.720 -10.6031.00 64.85
ATOM 3963 0 GLU C 55 64.879 213.577 -10.8561.00 65.18
ATOM 3964 N GLU C 56 66.501 125.128 -10.8241.00154.58
ATOM 3965 CA GLU C 56 67.494 114.226 -11.3971.00157.05
ATOM 3966 CB GLU C S6 67.211 113.985 -12.8821.00224.00
ATOM 3967 CG GLU C 56 67.245 115.244 -13.7181.00125.79
71

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ATOM 3968 CD GLU C 56 66.159 116.225 -13.323 1.00127.18
6
ATOM 3969 OE1 GLU C 56 64.979 115.979 -13.655 1.00127.61
8
ATOM 3970 OE2 GLU C 56 66.484 117.239 -12.667 1.00127.56
8
ATOM 3971 C GLU C 56 68.891 114.795 -11.240 1.00158.22
6
ATOM 3972 0 GLU C 56 69.172 115.529 -10.294 1.00158.76
8
ATOM 3973 N GLY C 57 69.765 114.445 -12.176 1.00103.07
7
ATOM 3974 CA GLY C 57 71.129 114.932 -12.130 1.00104.55
6
ATOM 3975 C GLY C 57 71.238 116.354 -12.641 1.00105.62
6
ATOM 3976 0 GLY C 57 70.732 116.685 -13.720 1.00106.41
8
ATOM 3977 N ALA C 58 71.898 117.202 -11.860 1.00132.92
7
ATOM 3978 CA ALA C 58 72.075 118.592 -12.240 1.00133.77
6
ATOM 3979 CB ALA C 58 72.757 118.657 -13.590 1.00 19.77
6
ATOM 3980 C ALA C 58 70.722 119.303 -12.289 1.00134.75
6
ATOM 3981 0 ALA C 58 69.912 119.053 -13.187 1.00134.98
8
ATOM 3982 N LYS C 59 70.492 120.188 -11.318 1.00156.20
7
ATOM 3983 CA LYS C 59 69.246 120.950 -11.200 1.00157.09
6
ATOM 3984 CB LYS C 59 69.438 122.137 -10.246 1.00107.35
6
ATOM 3985 CG LYS C 59 69.663 121.752 -8.784 1.00107.22
6
ATOM 3986 CD LYS C 59 69.714 122.989 -7.877 1.00106.92
6
ATOM 3987 CE LYS C 59 69.795 122.617 -6.393 1.00106.52
6
ATOM 3988 NZ LYS C 59 69.818 123.818 -5.509 1.00105.42
7
ATOM 3989 C LYS C 59 68.684 121.463 -12.524 1.00157.61
6
ATOM 3990 0 LYS C 59 69.074 122.530 -13.006 1.00157.43
8
ATOM 3991 N GLY C 60 67.752 120.701 -13.094 1.00129.14
7
ATOM 3992 CA GLY C 60 67.137 121.081 -14.353 1.00129.03
6
ATOM 3993 C GLY C 60 68.148 121.434 -15.429 1.00129.06
6
ATOM 3994 O GLY C 60 68.306 122.606 -15.779 1.00129.14
8
ATOM 3995 N LYS C 61 68.832 120.419 -15.952 1.00189.07
7
ATOM 3996 CA LYS C 61 69.837 120.612 -16.997 1.00188.42
6
ATOM 3997 CB LYS C 61 71.193 120.067 -16.531 1.00 95.41
6
ATOM 3998 CG LYS C 61 71.836 120.846 -15,.3971.00 95.06
6
ATOM 3999 CD LYS C 61 72.555 122.091 -15.882 1.00 94.98
6
ATOM 4000 CE LYS C 61 73.320 122.758 -14.738 1.00 94.81
6
ATOM 4001 NZ LYS C 61 74.117 123.930 -15.204 1.00 94.67
7
ATOM 4002 C LYS C 61 69.433 119.927 -18.304 1.00187.52
6
ATOM 4003 O LYS C 61 .69.906 120.301 -19.380 1.00187.21
8
ATOM 4004 N GLY C 62 68.561 118.927 -18.207 1.00173.41
7
ATOM 4005 CA GLY C 62 68.121 118.212 -19.394 1.00172.55
6
ATOM 4006 C GLY C 62 66.715 117.645 -19.301 1.00171.94
6
ATOM 4007 O GLY C 62 65.782 118.334 -18.882 1.00172.35
8
ATOM 4008 N GLY C 63 66.562 116.382 -19.692 1.00106.71
7
ATOM 4009 CA GLY C 63 65.255 115.752 -19.651 1.00104.35
6
ATOM 4010 C GLY C 63 65.002 114.888 -18.430 1.00102.47
6
ATOM 4011 0 GLY C 63 65.893 114.685 -17.604 1.00102.37
8
ATOM 4012 N LEU C 64 63.769 114.394 -18.330 1.00153.39
7
ATOM 4013 CA LEU C 64 63.305 113.529 -17.240 1.00151.74
6
ATOM 4014 CB LEU C 64 64.362 112.473 -16.911 1.00130.45
6
ATOM 4015 CG LEU C 64 64.731 111.550 -18.072 1.00129.19
6
ATOM 4016 CD1 LEU C 64 65.827 110.609 -17.627 1.00128.90
6
ATOM 4017 CD2 LEU C 64 63.509 110.775 -18.533 1.00129.76
6
ATOM 4018 C LEU C 64 62.891 114.261 -15.965 1.00150.41
6
ATOM 4019 O LEU C 64 63.715 114.536 -15.097 1.00150.11
8
ATOM 4020 N VAL C 65 61.599 114.564 -15.864 1.00 94.25
7
ATOM 4021 CA VAL C 65 61.043 115.254 -14.703 1.00 93.03
6
ATOM 4022 CB VAL C 65 60.210 116.477 -15.131 1.00 49.35
6
ATOM 4023 CG1 VAL C 65 59.347 116.972 -13.990 1.00 48.78
6
72

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4024 CG2 VAL C 65 61.131 117.571-15.572 1.00 49.43
6
ATOM 4025 C VAL C 65 60.142 114.304-13.925 1.00 93.26
6
ATOM 4026 O VAL C 65 60.385 114.015-12.747 1.00 93.73
8
ATOM 4027 N LEU C 66 59.099 113.828-14.601 1.00 62.83
7
ATOM 4028 CA LEU C 66 58.137 112.902-14.017 1.00 60.91
6
ATOM 4029 CB LEU C 66 58.833 111.620-13.543 1.00 55,02
6
ATOM 4030 CG LEU C 66 57.939 110.554-12.900 1.00 54.34
6
ATOM 4031 CD1 LEU C 66 56.776 210.172-13.817 1.00 53.57
6
ATOM 4032 CD2 LEU C 66 58.798 109.350-12.578 1.00 54.77
6
ATOM 4033 C LEU C 66 57.368 113.504-12.867 1.00 59.76
6
ATOM 4034 O LEU C 66 57.90 113.717-11.780 1.00 58.86
8
ATOM 4035 N ASP C 67 56.101 113.786-13.125 1.00 83.71
7
ATOM 4036 CA ASP C 67 55.234 114.338-12.111 1.00 84.15
6
ATOM 4037 CB ASP C 67 54.619 115.662-12.588 1.00162.72
6
ATOM 4038 CG ASP C 67 54.463 115.733-14.100 1.00164.62
6
ATOM 4039 OD1 ASP C 67 55.465 115.526-14.817 1.00164.61
8
ATOM 4040 OD2 ASP C 67 53.339 116.011-14.570 1.00166.53
8
ATOM 4041 C ASP C 67 54.164 113.299-12.820 1.00 83.51
6
ATOM 4042 0 ASP C 67 54.057 112.295-12.531 1.00 83.41
8
ATOM 4043 N PHE C 68 53.390 113.526-10.765 1.00 28.71
7
ATOM 4044 CA PHE C 68 52.343 112.596-10.385 1.00 28.07
6
ATOM 4045 CB PHE C 68 52.508 112.183-8.912 1.00 91.06
6
ATOM 4046 CG PHE C 68 53.892 111.617-8.571 2.00 92.26
6
ATOM 4047 CD1 PHE C 68 55.010 112.452-8.483 1.00 92.24
6
ATOM 4048 CD2 PHE C 68 54.069 110.251-8.321 1.00 91.63
6
ATOM 4049 CE1 PHE C 68 56.269 111.932-8.150 1.00 91.63
6
ATOM 4050 CE2 PHE C 68 55.327 109.731-7.989 1.00 90.24
6
ATOM 4051 CZ PHE C 68 56.422 120.571-7.904 1.00 90.55
6
ATOM 4052 C PHE C 68 50.983 113.236-10.621 1.00 27.88
6
ATOM 4053 O PHE C 68 50.844 114.454-10.543 1.00 26.24
8
ATOM 4054 N LEU C 69 49.993 112.405-10.941 1.00131.88
7
ATOM 4055 CA LEU C 69 48.627 112.862-11.193 1.00132.72
6
ATOM 4056 CB LEU C 69 48.188 112.515-12.617 1.00 63.15
6
ATOM 4057 .CG LEU C 69 49.022 112.904-13.834 1.00 63.27
6
ATOM 4058 CD1 LEU C 69 48.220 112.557-15.096 1.00 62.57
6
ATOM 4059 CD2 LEU C 69 49.365 114.391-13.795 1.00 63.36
6
ATOM 4060 C LEU C 69 47.668 112.176-10.221 1.00133.30
6
ATOM 4061 O LEU C 69 47.779 112.332-9.002 1.00134.07
8
ATOM 4062 N GLU C 70 46.730 111.410-10.775 1.00 73.64
7
ATOM 4063 CA GLU C 70 45.758 110.691-9.966 1.00 74.13
6
ATOM 4064 CB GLU C 70 44.628 110.115-10.825 1.00 82.92
6
ATOM 4065 CG GLU C 70 44.808 110.252-12.315 1.00 84.69
6
ATOM 4066 CD GLU C 70 44.267 111.559-12.825 1.00 85.45
6
ATOM 4067 OE1 GLU C 70 44.775 112.616-12.402 1.00 85.54
8
ATOM 4068 OE2 GLU C 70 43.328 111.525-13.643 1.00 86.32
8
ATOM 4069 C GLU C 70 46.387 109.546-9.192 1.00 73.87
6
ATOM 4070 O GLU C 70 47.593 109.341-9.202 1.00 74.29
8
ATOM 4071 N TYR C 71 45.525 108.794-8.534 1.00 41.60
7
ATOM 4072 CA TYR C 71 45.908 107.657-7.720 1.00 41.39
6
ATOM 4073 CB TYR C 71 46.016 108.097-6.254 1.00 96.85
6
ATOM 4074 CG TYR C 71 44.711 108.649-5.736 1.00 99.37
6
ATOM 4075 CD1 TYR C 71 43.794 107.826-5.096 1.00100.19
6
ATOM 4076 CE1 TYR C 71 42.526 108.288-4.753 1.00101.02
6
ATOM 4077 CD2 TYR C 71 44.332 109.963-6.011 1.00100.76
6
ATOM 4078 CE2 TYR C 71 43.061 110.437-5.672 1.00101.32
6
ATOM 4079 CZ TYR C 71 42.160 109.592-5.045 1.00101.36
6
73

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4080 OH TYR C 71 40.894 110.046-4.732 1.00100.65
ATOM 4081 C TYR C 71 44.721 106.725-7.905 1.00 40.46
ATOM 4082 0 TYR C 71 43.591 107.193-8.038 1.00 39.16
ATOM 4083 N ARG C 72 44.944 105.419-7.911 1.00 55.34
ATOM 4084 CA ARG C 72 43.812 104.538-8.107 1.00 55.85
ATOM 4085 CB ARG C 72 43.737 104.137-9.585 1.00 97.22
ATOM 4086 CG ARG C 72 42.499 103.325-9.949 1.00 99.67
ATOM 4087 CD ARG C 72 42.447 102.996-11.431 1.00100.61
ATOM 4088 NE ARG C 72 41.858 101.682-11.663 1.00102.71
ATOM 4089 CZ ARG C 72 41.921 101.022-12.815 1.00104.14
ATOM 4090 NH1 ARG C 72 42.548 101.551-13.856 1.00103.10
ATOM 4091 NH2 ARG C 72 41.366 99.820 -12.921 1.00105.69
ATOM 4092 C ARG C 72 43.736 103.295-7.230 1.00 55.38
ATOM 4093 0 ARG C 72 44.406 102.293-7.487 1.00 55.48
ATOM 4094 N ILE C 73 42.924 103.368-6.184 1.00 45.89
ATOM 4095 CA ILE C 73 42.716 102.212-5.321 1.00 46.35
ATOM 4096 CB ILE C 73 42.063 102.615-3.953 1.00 79.44
ATOM 4097 CG2 ILE C 73 41.576 101.386-3.206 1.00 79.78
ATOM 4098 CG1 ILE C 73 43.061 103.371-3.082 1.00 79.96
ATOM 4099 CD1 ILE C 73 43.415 104.721-3.606 1.00 82.19
ATOM 4100 C ILE C 73 41.683 101.486-6.171 1.00 45.72
ATOM 4101 0 ILE C 73 41.190 102.072-7.124 1.00 45.98
ATOM 4102 N GLY C 74 41.347 100.238-5.877 1.00 59.70
ATOM 4103 CA GLY C 74 40.325 99.618 -6.699 1.00 59.89
ATOM 4104 C GLY C 74 40.210 98.115 -6,685 1.00 59.31
ATOM 4105 O GLY C 74 40.582 97.464 -5,715 1.00 59.22
ATOM 4106 N ASP C 75 39.676 97.580 -7,780 1.00 75.15
ATOM 4107 CA ASP C 75 39.494 96.144 -7.966 1.00 74.49
ATOM 4108 CB ASP C 75 39.890 95.732 -9.426 1.00 60.41
ATOM 4109 CG ASP C 75 40.785 96.777 -10,166 1.00 61.12
ATOM 4110 OD1 ASP C 75 40.351 97.924 -10,406 1.00 61.97
ATOM 4111 OD2 ASP C 75 41.926 96.433 -10.540 1.00 60.97
ATOM 4112 C ASP C 75 40.230 95.254 -6.930 1.00 73.41
ATOM 4113 0 ASP C 75 41.259 94.642 -7.233 1.00 73.61
ATOM 4114 N PRO C 76 39.690 95.157 -5,697 1.00 53.20
ATOM 4115 CD PRO C 76 38.342 95.596 -5.308 1.00 76.69
ATOM 4116 CA PRO C 76 40.293 94.349 -4.626 1.00 52.08
ATOM 4117 CB PRO C 76 39.281 94.456 -3.495 1.00 75.29
ATOM 4118 CG PRO C 76 38.002 94.598 -4.222 1.00 76.95
ATOM 4119 C PRO C 76 40.535 92.907 -5,039 1.00 50.99
ATOM 4120 0 PRO C 76 39.833 92.378 -5.887 1.00 52.01
ATOM 4121 N PRO C 77 41.541 92.253 -4.441 1.00 43.95
ATOM 4122 CD PRO C 77 42.534 92.871 -3.546 1.00100.31
ATOM 4123 CA PRO C 77 41.910 90.863 -4.729 1.00 42.97
ATOM 4124 CB PRO C 77 43.053 90.605 -3.752 1.00 99.15
ATOM 4125 CG PRO C 77 43.722 91.936 -3.691 1.00100.25
ATOM 4126 C PRO C 77 40.805 89.821 -4.615 1.00 42.68
ATOM 4127 0 PRO C 77 40.045 89.616 -5.559 1.00 42.66
ATOM 4128 N PHE C 78 40.726 89.162 -3.460 1.00105:15
ATOM 4129 CA PHE C 78 39.736 88.111 -3.235 1.00104.28
ATOM 4130 CB PHE C 78 40.370 86.947 -2.479 1.00 44.72
ATOM 4131 CG PHE C 78 .~ 41.52886.3'41-3.185 1.00 41.61
ATOM 4132 CD1 PHE C 78 42.663 87.091 -3.439 1.00 40.07
ATOM 4133 CD2 PHE C 78 41.479 85.023 -3.611 1.00 40.88
ATOM 4134 CE1 PHE C 78 43.735 86.542 -4.111 1.00 40.12
ATOM 4135 CE2 PHE C 78 42.544 84.456 -4.284 1.00 41.19
74

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4136 CZ PHE C 78 43.680 85.216 -4.537 1.00 40.97
6
ATOM 4137 C PHE C 78 38.513 88.571 -2.478 1.00 104.94
6
ATOM 4138 0 PHE C 78 37.586 89.119 -3.068 1.00106.80
8
ATOM 4139 N SER C 79 38.510 88.331 -1.170 1.00 30.96
7
ATOM 4140 CA SER C 79 37.382 88.729 -0.336 1.00 31.37
6
ATOM 4141 CB SER C 79 36.118 88.019 -0.803 1.00 97.02
6
ATOM 4142 OG SER C 79 36.158 86.651 -0.430 1.00 95.83
8
ATOM 4143 C SER C 79 37.556 88.437 1.145 1.00 32.21
6
ATOM 4144 0 SER C 79 38.066 87.393 1.514 1.00 32.04
8
ATOM 4145 N GLN C 80 37.113 89.353 1.997 1.00 71.63
7
ATOM 4146 CA GLN C 80 37.202 89.121 3.429 1.00 73.84
6
ATOM 4147 CB GLN C 80 36.353 90.134 4.188 1.00 75.43
6
ATOM 4148 CG GLN C 80 36.884 91.544 4.141 1.00 75.54
6
ATOM 4149 CD GLN C 80 36.155 92.451 5.104 1.00 75.38
6
ATOM 4150 OE1 GLN C 80 35.547 91.989 6.067 1.00 74.86
8
ATOM 4151 NE2 GLN C 80 36.227 93.752 4.862 1.00 75.75
7
ATOM 4152 C GLN C 80 36.653 87.715 3.660 1.00 74.89
6
ATOM 4153 0 GLN C 80 35.756 87.278 2.936 1.00 74.90
8
ATOM 4154 N ASP C 81 37.184 87.016 4.661 1.00 54.21
7
ATOM 4155 CA ASP C 81 36.764 85.645 4.961 1.00 55.86
6
ATOM 4156 CB ASP C 81 35.256 85.457 4.708 1.00 95.24
~ 6
ATOM 4157 CG ASP C 81 34.424 85.514 5.985 1.00 96.12
6
ATOM 4158 OD1 ASP C 81 33.181 85.604 5.875 1.00 95.54
8
ATOM 4159 OD2 ASP C 81 35.001 85.453 7.095 1.00 96.33
8
ATOM 4260 C ASP C 81 37.560 84.738 4.031 1.00 56.84
6
ATOM 4161 0 ASP C 81 38.147 83.743 4.462 1.00 57.38
8
ATOM 4162 N GLU C 82 37.568 85.097 2.749 1.00 54.96
7
ATOM 4163 CA GLU C 82 38.301 84.354 1.729 1:00 56.27
6
ATOM 4164 CB GLU C 82 37.992 84.933 0.347 1.00 93.25
6
ATOM 4165 CG GLU C 82 38.317 84.045 -0.837 1.00 95.92
6
ATOM 4166 CD GLU C 82 38.033 84.733 -2.169 1.00 97.60
6
ATOM 4167 OE1 GLU C 82 37.052 85.501 -2.254 1.00 96.68
8
ATOM 4268 OE2 GLU C 82 38.783 84.497 -3.142 2.00 99.04
8
ATOM 4169 C GLU C 82 39.773 84.567 2.062 1.00 56.43
6
ATOM 4170 0 GLU C 82 40.643 83.786 1.670 1.,0056.69
8
ATOM 4171 N CYS C 83 40.035 85.643 2.800 1.00 46.10
. 7
ATOM 4172 CA CYS C 83 41.387 85.992 3.195 1.00 46.27
6
ATOM 4173 CB CYS C 83 41.488 87.491 3.473 1.00 42.68
6
ATOM 4174 SG CYS C 83 42.080 88.510 2.098 1.00 46.55
16
ATOM 4175 C CYS C 83 41.767 85.219 4.435 1.00 46.32
6
ATOM 4176 0 CYS C 83 42.624 84.341 4.384 1.00 47.41
8
ATOM 4177 N ARG C 84 41.115 85.549 5.546 1.00 84.05
7
ATOM 4178 CA ARG C 84 41.360 84.909 6.836 1.00 84.14
6
ATOM 4179 CB ARG C 84 40.213 85.248 7.788 1.00 63.56
6
ATOM 4180 CG ARG C 84 39.841 86.730 7.773 1.00 65.71
6
ATOM 4181 CD ARG C 84 38.499 87.020 8.456 1.00 66.90
6
ATOM 4182 NE ARG C 84 38.042 88.390 8.205 1.00 67.15
7
ATOM 4183 CZ ARG C 84 36.914 88.897 8.684 1.00 67.18
6
ATOM 4184 NH1 ARG C 84 36.131 88.145 9.436 1.00 67.91
7
ATOM 4185 NH2 ARG C 84 36.581 90.153 8.425 1.00 66.18
7
ATOM 4186 C ARG C 84 41.471 83.395 6.668 1.00 83.47
6
ATOM 4187 0 ARG C 84 42.113 82.704 7.458 1.00 .83.60
8
ATOM 4188 N GLU C 85 40.839 82.892 5.616 1.00 51.89
7
ATOM 4189 CA GLU C 85 40.845 81.473 5.317 1.00 50.77
6
ATOM 4190 CB GLU C 85 39.711 81.162 4.337 1.00110.83
6
ATOM 4191 CG GLU C 85 39.405 79.694 4.168 1.00114.03
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4192 CD GLU C 85 37.971 79.454 3.741 1.00116.33
6
ATOM 4193 OE1 GLU C 85 37.566 79.951 2.665 1.00117.61
8
ATOM 4194 OE2 GLU C 85 37.243 78.768 4.491 1.00118.24
8
ATOM 4195 C GLU C 85 42.190 81.090 4.724 1.00 48.87
6
ATOM 4196 O GLU C 85 42.891 80.238 5.268 1.00 48.42
8
ATOM 4197 N LYS C 86 42.542 82.755 3.623 1.00 42.41
7
ATOM 4198 CA LYS C 86 43.788 81.515 2.891 1.00 41.09
6
ATOM 4199 CB LYS C 86 43.529 81.694 1.397 1.00 51.50
6
ATOM 4200 CG LYS C 86 42.664 80.608 0.806 1.00 51.79
6
ATOM 4201 CD LYS C 86 42.216 80.915 -0.609 1.00 52.63
6
ATOM 4202 CE LYS C 86 41.368 79.761 -1.135 1.00 53.06
6
ATOM 4203 NZ LYS C 86 40.609 80.092 -2.375 1.00 52.14
7
ATOM 4204 C LYS C 86 44.974 82.391 3.312 1.00 40.46
6
ATOM 4205 0 LYS C 86 45.927 82.576 2.541 1.00 39.36
8
ATOM 4206 N ASP C 87 44.912 82.909 4.541 1.00 49.99
7
ATOM 4207 CA ASP C 87 45.959 83.770 5.094 1.00 48.35
6
ATOM 4208 CB ASP C 87 47.226 82.961 5.387 1.00 56.86
6
ATOM 4209 CG ASP C 87 47.079 82.066 6.599 1.00 56.33
6
ATOM 4210 OD1 ASP C 87 46.566 82.549 7.625 1.00 56.05
8
ATOM 4211. 0D2 ASP C 87 47.490 80.887 6.533 1.00 57.01
8
ATOM 4212 C ASP C 87 46.309 84.936 4.177 1.00 47.56
6
ATOM 4213 0 ASP C 87 47.477 85.287 4.018 1.00 47.08
8
ATOM 4214 N LEU C 88 45.293 85.541 3.578 1.00 38.58
7
ATOM 4215 CA LEU C 88 45.526 86.659 2.690 1.00 37.19
6
ATOM 4216 CB LEU C 88 44.652 86.529 1.451 1.00 93.94
6
ATOM 4217 CG LEU C 88 44.885 85.243 0.658 1.00 94.32
6
ATOM 4218 CD1 LEU C 88 44.093 85.334 -0.633 1.00 95.01
6
ATOM 4219 CD2 LEU C 88 46.372 85.040 0.364 1.00 94.63
6
ATOM 4220 C LEU C 88 45.277 87.994 3.376 1.00 36.02
6
ATOM 4221 0 LEU C 88 44.302 88.161 4.098 1.00 35.88
8
ATOM 4222 N THR C 89 46.184 88.935 3.146 1.00 29.88
7
ATOM 4223 CA THR C 89 46.094 90.259 3.731 1.00 29.65
6
ATOM 4224 CB THR C 89 47.479 90.955 3.737 1.00118.66
6
ATOM 4225 OG1 THR C 89 48.448 90.092 4.345 1.00120.67
8
ATOM 4226 CG2 THR C 89 47.422 92.265 4.515 1.00119.01
6
ATOM 4227 C THR C 89 45.129 91.101 2.906 1.00 28.47
6
ATOM 4228 0 THR C 89 45.539 91.743 1.946 1.00 28.45
8
ATOM 4229 N TYR C 90 43.852 91.089 3.281 1.00 32.04
7
ATOM 4230 CA TYR C 90 42:807 91.852 2.595 1.00 30.45
6
ATOM 4231 CB TYR C 90 41.619 92.044 3.536 1.00 59.69
6
ATOM 4232 CG TYR C 90 40.472 92.807 2.931 1.00 58.99
6
ATOM 4233 CD1 TYR C 90 39.680 92.242 1.938 1.00 58.51
6
ATOM 4234 CE1 TYR C 90 38.638 92.949 1.364 1.00 58.19
6
ATOM 4235 CD2 TYR C 90 40.189 94.101 3.336 1.00 58.79
6
ATOM 4236 CE2 TYR C 90 39.152 94.813 2.769 1.00 58.09
6
ATOM 4237 CZ TYR C 90 38.382 94.234 1.785 1.00 57.73
6
ATOM 4238 OH TYR C 90 37.358 94.956 1.225 1.00 58.50
8
ATOM 4239 C TYR C 90 43.308 93.218 2.149 1.00 30.34
6
ATOM 4240 0 TYR C 90 43,250 94.183 2.917 1.00 29.90
8
ATOM 4241 N GLN C 91 43.789 93.298 0.909 1.00 80.36
7
ATOM 4242 CA GLN C 91 44.322 94.540 0.357 1.00 80.21
6
ATOM 4243 CB GLN C 91 45,760 94.330 -0.108 1.00 69.15
6
ATOM 4244 CG GLN C 91 46.719 93.994 1.005 1.00 70.45
6
ATOM 4245 CD GLN C 91 48.044 93.506 0.481 1.00 71.04
6
ATOM 4246 OE1 GLN C 91 48.086 92.670 -0.422 1.00 70,94
8
ATOM 4247 NE2 GLN C 91 49.140 94.014 1.049 1.00 71,35
7
76

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4248 C GLN C 91 43.506 95.051 -0.812 1.00 79.27
6
ATOM 4249 0 GLN C 91 42.302 94.845 -0.875 1.00 79.86
8
ATOM 4250 N ALA C 92 44.180 95.728 -1.734 1.00 69.38
7
ATOM 4251 CA ALA C 92 43.550 96.274 -2.931 1.00 69.05
6
ATOM 4252 CB ALA C 92 42.455 97.270 -2.552 1.00 13.87
6
ATOM 4253 C ALA C 92 44.610 96.946 -3.805 1.00 68.88
6
ATOM 4254 O ALA C 92 45.516 97.616 -3.305 1.00 68.52
8
ATOM 4255 N PRO C 93 44.507 96.771 -5.128 1.00 48.77
7
ATOM 4256 CD PRO C 93 43.396 96.091 -5.813 1.00110.54
6
ATOM 4257 CA PRO C 93 45.441 97.342 -6.100 1.00 48.32
6
ATOM 4258 CB PRO C 93 44.984 96.721 -7.413 1.00110.46
6
ATOM 4259 CG PRO C 93 43.506 96.638 -7.219 2.00111.39
6
ATOM 4260 C PRO C 93 45.443 98.869 -6.162 1.00 47.83
6
ATOM 4261 0 PRO C 93 44.432 99.506 -6.501 1.00 48.40
8
ATOM 4262 N LEU C 94 46.589 99.453 -5.839 1.00 48.05
7
ATOM 4263 CA LEU C 94 46.722 100.894-5.878 1.00 46.69
6
ATOM 4264 CB LEU C 94 47.059 101.446-4.485 1.00 79.69
6
ATOM 4265 CG LEU C 94 47.462 102.932-4.429 1.00 79.83
6
ATOM 4266 CD1 LEU C 94 46.313 103.800-4.898 1.00 79.99
6
ATOM 4267 CD2 LEU C 94 47.854 103.318-3.022 1.00 79.90
6
ATOM 4268 C LEU C 94 47.796 101.337-6.859 1.00 45.26
6
ATOM 4269 O LEU C 94 48,969 101.386-6.503 1.00 44.54
8
ATOM 4270 N TYR C 95 47.416 101.644-8.096 1.00 34.68
7
ATOM 4271 CA TYR C 95 48.411 102.135-9.042 1.00 34.95
6
ATOM 4272 CB TYR C 95 48.266 101.458-10.425 1.00 51.57
6
ATOM 4273 CG TYR C 95 47.026 100.607-10.637 1.00 52.57
6
ATOM 4274 CD1 TYR C 95 45.768 101.190-10.768 1.00 53.23
6
ATOM 4275 CE1 TYR C 95 44.624 100.414-10.987 1.00 52.69
6
ATOM 4276 CD2 TYR C 95 47,118 99.214 -10.726 1.00 53.25
6
ATOM 4277 CE2 TYR C 95 45.975 98.418 -10.939 1.00 53.54
6
ATOM 4278 CZ TYR C 95 44.725 99.030 -11.069 1.00 52.95
6
ATOM 4279 OH TYR C 95 43.579 98.269 -11.273 1.00 53.39
8
ATOM 4280 C TYR C 95 48.287 103.675-9.148 1.00 34.90
6
ATOM 4281 O TYR C 95 47.259 104.244-8.759 1.00 33.96
8
ATOM 4282 N ALA C 96 49.333 104.342-9.647 1.00 29.90
7
ATOM 4283 CA ALA C 96 49.324 105.802-9.780 1.00 31.71
6
ATOM 4284 CB ALA C 96 50.085 106.414-8.621 1.00108.85
6
ATOM 4285 C ALA C 96 49.881 106.341-11.109 1.00 33.12
6
ATOM 4286 O ALA C 96 51.021 106.065-11.468 1.00 33.20
8
ATOM 4287 N ARG C 97 49.077 107.144-11.807 1.00 56.27
7
ATOM 4288 CA ARG C 97 49.432 107.744-13.108 1.00 57.36
6
ATOM 4289 CB ARG C 97 48.184 108.368-13.735 1.00 69.99
6
ATOM 4290 CG ARG C 97 46.984 107.450-13.842 1.00 71.50
6
ATOM 4291 CD ARG C 97 45.935 108.071-14.752 1.00 72.07
6
ATOM 4292 NE ARG C 97 46.567 208.672-15.922 1.00 73.73
7
ATOM 4293 CZ ARG C 97 45.930 108.991-17.041 1.00 74.74
6
ATOM 4294 NH1 ARG C 97 44.629 108.762-17.151 1.00 75.60
7
ATOM 4295 NH2 ARG C 97 46.596 109.540-18.049 1.00 74.96
7
ATOM 4296 C ARG C 97 50.528 108.823-13.116 1.00 57.68
6
ATOM 4297 0 ARG C 97 50.278 109.958-12.710 1.00 56.82
8
ATOM 4298 N LEU C 98 51.716 108.495-13.618 1.00102.03
7
ATOM 4299 CA LEU C 98 52.818 109.467-13.668 1.00103.88
6
ATOM 4300 CB LEU C 98 54.054 108.888-12.963 1.00 55.34
6
ATOM 4302 CG LEU C 98 53.766 107.917-11.803 1.00 55.72
6
ATOM 4302 CD1 LEU C 98 55.067 107.471-11.165 1.00 56.45
6
ATOM 4303 CD2 LEU C 98 52.866 108.572-10.770 1.00 54.94
6
77

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4304 C LEU C 98 53.151 109.833-15.125 1.00104.43
ATOM 4305 O LEU C~98 52.603 109.236-16.050 2.00105.22
ATOM 4306 N GLN C 99 54.034 110.810-15.332 1.00 65.41
ATOM 4307 CA GLN C 99 54.407 111.243-16.688 1.00 65.55
ATOM 4308 CB GLN C 99 53.305 112.132-17.289 1.00 54.10
ATOM 4309 CG GLN C 99 52.742 113.191-16.329 1.00 54.45
.
ATOM 4310 CD GLN C 99 51.745 114.173-16.975 1.00 54.22
ATOM 4311 OE1 GLN C 99 50.946 113.799-17.836 1.00 53.31
ATOM 4312 NE2 GLN C 99 51.781 115.432-16.530 1.00 53.03
ATOM 4313 C GLN C 99 55.724 112.009-16.719 1.00 66.35
ATOM 4314 O GLN C 99 55.746 113.204-16.432 1.00 67.40
ATOM 4315 N LEU C 100 56.815 111.337-17.083 1.00 57.89
ATOM 4316 CA LEU C 100 58.134 111.988-17.121 1.00 58.25
ATOM 4317 CB LEU C 200 59.253 110.949-27.182 1.00 58.65
ATOM 4318 CG LEU C 100 58.824 109.541-17.579 1.00 59.06
ATOM 4319 CD1 LEU C 100 58.250 109.558-18.983 1.00 59.44
ATOM 4320 CD2 LEU C 100 60.013 108.607-17.468 1.00 58.33
ATOM 4321 C LEU C 100 58.312 112.984-18.247 1.00 59.20
ATOM 4322 O LEU C 100 57.903 112.741-19.382 1.00 59.43
ATOM 4323 N ILE C 101 58.931 114.113-17.920 1.00 72.44
ATOM 4324 CA ILE C 101 59.157 115.174-18.891 1.00 74.12
ATOM 4325 CB ILE C 101 58.997 116.566-18.227 1.00 59.88
ATOM 4326 CG2 ILE C 101 58.873 117.647-19.292 1.00 59.71
ATOM 4327 CG1 ILE C 101 57.758 116.584-17.326 1.00 60.33
ATOM 4328 CD1 ILE C 101 56.461 116.261-18.039 1.00 61.69
ATOM 4329 C ILE C 101 60.563 115.050-19.479 1.00 75.54
ATOM 4330 O ILE C 101 61.377 114.275-18.986 1.00 75.14
ATOM 4331 N HIS C 102 60.835 115.810-20.538 1.00 74.04
ATOM 4332 CA HIS C 102 62.136 115.798-21.202 1.00 75.31
ATOM 4333 CB HIS C 102 62.010 115.223-22.619 1.00 83.57
ATOM 4334 CG HIS C 102 61.650 113.771-22.670 1.00 84.63
ATOM 4335 CD2 HIS C 102 60.613 113.131-23.260 1.00 84.82
ATOM 4336 ND1 HIS C 102 62.438 112.788-22.112 1.00 85.38
ATOM 4337 CE1 HIS C 102 61.903 111.604-22.358 1.00 85.82
ATOM 4338 NE2 HIS C 102 60.795 111.784-23.054 1.00 85.53
ATOM 4339 C HIS C 102 62.722 117.210-21.321 1.00 75.87
ATOM 4340 O HIS C 102 62.562 118.066-20.441 1.00 75.97
ATOM 4341 N LYS C 103 63.403 117.417-22.443 1.00207.67
ATOM 4342 CA LYS C 103 64.040 118.676-22.813 1.00108.49
ATOM 4343 CB LYS C 103 65.306 118.919-21.983 1.00112.21
ATOM 4344 CG LYS C 103 65.185 120.085-21.008 1.00113.26
ATOM 4345 CD LYS C 103 ~ 64.739 121.354-21.723 1.00113.90
ATOM 4346 CE LYS C 103 64.448 122.480-20.744 2.00114.86
ATOM 4347 NZ LYS C 103 63.685 123.587-21.394 1.00115.30
ATOM 4348 C LYS C 103 64.404 118.508-24.286 1.00108.56
ATOM 4349 O LYS C 103 65.258 119.216-24.830 1.00108.10
ATOM 4350 N ASP C 104 63.727 117.548-24.911 1.00 51.37
ATOM 4351 CA ASP C 104 63.928 117.196-26.307 1.00 51.13
ATOM 4352 CB ASP C 104 65.022 116.117-26:414 1.00 80.96
ATOM 4353 CG ASP C 104 64.948 115.070-25.287 1.00 81.71
ATOM 4354 OD1 ASP C 104 64.879 115.445-24.095 1.00 81.16
ATOM 4355 OD2 ASP C 104 64.979 113.858-25.586 1.00 83.61
ATOM 4356 C ASP C 104 62.600 116.702-26.880 1.00 51.12
ATOM 4357 O ASP C 104 62.547 115.702-27.605 1.00 50.09
ATOM 4358 N THR C 105 61.532 117.422-26.534 1.00153.67
ATOM 4359 CA THR C 105 60.174 117.115-26.984 1.00156.13
78

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4360 CB THR C205 60.056 117.234-28.533 1.00130.99 6
ATOM 4361 OG1 THR C105 60.166 118.612-28.915 1.00131.67 8
ATOM 4362 CG2 THR C105 58.714 116.681-29.027 1.00130.54 6
ATOM 4363 C THR C105 59.687 115.734-26.547 1.00157.07 6
ATOM 4364 0 THR C105 60.143 114.707-27.063 1.00157.74 8
ATOM 4365 N GLY C106 58.753 115.718-25.595 1.00118.28 7
ATOM 4366 CA GLY C106 58.215 114.459-25.111 1.00117.30 6
ATOM 4367 C GLY C106 57.591 114.516-23.730 1.00116.71 6
ATOM 4368 O GLY C106 58.187 115.024-22.778 1.00116.63 8
ATOM 4369 N LEU C107 56.381 113.978-23.628 1.00 62.99 7
ATOM 4370 CA LEU C107 55.641 113.940-22.373 1.00 62.42 6
ATOM 4371 CB LEU C107 54.618 115.078-22.345 1.00116.78 6
ATOM 4372 CG LEU C107 55.122 116.457-22.787 2.00116.42 6
ATOM 4373 CD1 LEU C107 53.989 117.459-22.664 1.00116.42 6
ATOM 4374 CD2 LEU C107 56.303 116.890-21.940 1.00116.89 6
ATOM 4375 C LEU C107 54.931 112.583-22.236 1.00 62.45 6
ATOM 4376 0 LEU C107 53.711 112.475-22.414 1.00 61.96 8
ATOM 4377 N ILE C108 55.717 111.557-21.919 1.00 85.98 7
ATOM 4378 CA ILE C108 55.233 110.187-21.759 1.00 86.51 6
ATOM 4379 CB ILE C108 56.420 109.207-21.687 1.00 53.58 6
ATOM 4380 CG2 ILE C108 55.898 107.772-21.651 1.00 53.89 6
ATOM 4381 CG1 ILE C108 57.363 109.437-22.882 1.00 53.03 6
ATOM 4382 CD1 ILE C108 58.780 108.849-22.723 1.00 51.80 6
ATOM 4383 C ILE C108 54.393 110.024-20.493 1.00 87.01 6
ATOM 4384 O- ILE C208 54.701 110.625-19.460 1.00 86.91 8
ATOM 4385 N LYS C109 53.347 109.200-20.576 1.00 85.47 7
ATOM 4386 CA LYS C109 52.447 108.964-19.443 1.00 86.15 6
ATOM 4387 CB LYS C109 51.192 109.833-19.558 1.00111.00'6
ATOM 4388 CG LYS C109 51.422 111.320-19.741 1.00112.77 6
ATOM 4389 CD LYS C109 50.091 112.067-19.869 1.00112.61 6
ATOM 4390 CE LYS C109 49.182 111.816-18.664 1.00113.05 6
ATOM 4391 NZ LYS C109 47.901 112.576-18.738 1.00112.68 7
ATOM 4392 C LYS C109 51.967 107.523-19.336 1.00 86.43 6
ATOM 4393 O LYS C109 50.963 107.180-19.959 1.00 86.97 8
ATOM 4394 N GLU C110 52.649 106.684-18.555 1.00106.12 7
ATOM 4395 CA GLU C110 52.199 105.300-18.396 1.00106.53 6
ATOM 4396 CB GLU C110 53.252 104.422-17.717 1.00127.12 6
ATOM 4397 CG GLU C110 54.279 103.813-18.656 1.00129.05 6
ATOM 4398 CD GLU C110 53.646 103.074-19.818 1.00130.82 6
ATOM 4399 OE1 GLU C110 52.645 102.355-19.594 1.00131.80 8
ATOM 4400 OE2 GLU C110 54.156 103.203-20.955 1.00131.20 8
ATOM 4401 C GLU C110 50.941 105.288-17.545 1.00106.57 6
ATOM 4402 O GLU C110 50.976 105.636-16.363 1.00106.27 8
ATOM 4403 N ASP C111 49.829 104.889-18.153 1.00130.67 7
ATOM 4404 CA ASP C111 48.562 104.837-17.441 1.00130.36 6
ATOM 4405 CB ASP C111 47.443 104.314-18.349 1.00111.53 6
ATOM 4406 CG ASP C111 47.143 105.246-19.500 1.00112.76 6
ATOM 4407 OD1 ASP C111 46.999 106.462-19.257 1.00112.10 8
ATOM 4408 OD2 ASP C111 47.042 104.763-20.647 1.00114.07 8
ATOM 4409 C ASP C111 48.671 103.945-16.218 1.00129.01 6
ATOM 4410 O ASP C111 49.320 102.900-16.251 1.00128.94 8
ATOM 4411 N GLU C112 48.038 104.380-15.137 1.00 65.59 7
ATOM 4422 CA GLU C112 48.023 103.633-13.896 1.00 63.56 6
ATOM 4413 CB GLU C112 47.309 102.295-14.101 1.00 52.11 6
ATOM 4414 CG GLU C112 45.940 102.411-14.737 1.00 51.24 6
ATOM 4415 CD GLU C112 44.939 103.138-13.859 1.00 49.80 6
79

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4416 OE1 GLU C 112 44.595 102.600 -12.786 1.00 48.02
ATOM 4417 OE2 GLU C 112 44.494 104.242 -14.247 1.00 48.94
ATOM 4418 C GLU C 112 49.404 103.371 -13.320 1.00 62.52
ATOM 441,9 O GLU C 112 49.667 103.729 -12.174 1.00 63.01
ATOM 4420 N VAL C 113 50.288 102.757 -14.106 1.00 27.09
ATOM 4421 CA VAL C 113 51.614 102.422 -13.601 1.00 2'6.32
ATOM 4422 CB VAL C 113 52.356 103.659 -13.080 1.00 46.96
ATOM 4423 CG1 VAL C 113 53.837 103.351 -12.890 1.00 47.29
ATOM 4424 CG2 VAL C 113 52.129 104.821 -14.026 1.00 47.74
ATOM 4425 C VAL C 113 51.334 101.515 -12.408 1.00 26.34
ATOM 4426 0 VAL C 113 51.326 101.976 -11.264 1.00 26.39
ATOM 4427 N PHE C 114 51.078 100.236 -12.682 1.00 66.07
ATOM 4428 CA PHE C 114 50.770 99.254 -11.642 1.00 67.05
ATOM 4429 CB PHE C 114 50.988 97.828 -12.166 1.00208.87
ATOM 4430 CG PHE C 114 50.006 97.420 -13.235 1.00208.87
ATOM 4431 CD1 PHE C 114 50.049 98.002 -14.500 1.00208.87
ATOM 4432 CD2 PHE C 114 49.014 96.480 -12.966 1.00208.87
ATOM 4433 CE1 PHE C 114 49.119 97.658 -15.478 1.00208.87
ATOM 4434 CE2 PHE C 114 48.078 96.129 -13.940 1.00208.87
ATOM 4435 CZ PHE C 114 48.131 96.720 -15.198 1.00208.87
ATOM 4436 C PHE C 114 51.626 99.516 -10.423 1.00 66.08
ATOM 4437 0 PHE C 114 52.782 99.913 -10.552 1.00 65.65
ATOM 4438 N LEU'C 115 51.075 99.284 -9.238 1.00110.47
ATOM 4439 CA LEU C 115 51.837 99.586 -8.042 1.00109.25
ATOM 4440 CB LEU C 115 51.597 101.061 -7.725 1.00 37.12
ATOM 4441 CG LEU C 115 52.362 101.840 -6.671 1.00 37.25
ATOM 4442 CD1 LEU C 115 53.678 101.201 -6.264 1.00 37.12
ATOM 4443 CD2 LEU C 115 52.597 103.196 -7.286 1.00 38.33
ATOM 4444 C LEU C 115 51.578 98.695 -6.820 1.00108.54
ATOM 4445 0 LEU C 115 51.036 97.589 -6.948 1.00108:51
ATOM 4446 N GLY C 116 51.991 99.185 -5.647 1.00 46.71
ATOM 4447 CA GLY C 116 51.831 98.451 -4.404 1.00 44.97
ATOM 4448 C GLY C 116 50.410 98.185 -3.926 1.00 43.86
ATOM 4449 0 GLY C 116 49.435 98.802 -4.368 1.00 43.96
ATOM 4450 N HIS C 117 50.301 97.245 -2.997 1.00 38.74
ATOM 4451 CA HIS C 117 49.020 96.864 -2.432 1.00 37.35
ATOM 4452 CB HIS C 117 49.010 95.360 -2.145 1.00 67.60
ATOM 4453 CG HIS C 117 48.884 94.510 -3.372 1.00 68.06
ATOM 4454 CD2 HIS C 117 49.711 93.579 -3.899 1.00 67.77
ATOM 4455 ND1 HIS C 117 47.778 94.549 -4.195 1.00 68.70
ATOM 4456 CE1 HIS C 117 47.926 93.675 -5.173 1.00 67.88
ATOM 4457 NE2 HIS C 117 49.091 93.074 -5.017 1.00 68.90
ATOM 4458 C HIS C 117 48.730 97.64 -1.160 1.00 35.72
1
ATOM 4459 0 HIS C 117 49.639 97.971 -0.400 1.00 34.73
ATOM 4460 N LEU C 118 47.456 97.928 -0.931 1:00 74.84
ATOM 4461 CA LEU C 118 47.047 98.673 0.249 1.00 74.20
ATOM 4462 CB LEU C 118 46.449 100.027 -0.161 1.00 36.96
ATOM 4463 CG LEU C 118 45.850 101.004 0.861 1.00 36.31
ATOM 4464 CD1 LEU C 118 45.481 102.272 0.131 1.00 36.65
ATOM 4465 CD2 LEU C 118 44.619 100.426 1.531 1.00 35.26
ATOM 4466 C LEU C 118 46.017 97.889 1.037 1.00 73.30
ATOM 4467 0 LEU C 118 44.989 97.482 0.492 1.00 73.60
ATOM 4468 N PRO C 119 46.292 97.637 2.326 1.00 28.71
ATOM 4469 CD PRO C 119 47.628 97.637 2.960 1.00 31.16
ATOM 4470 CA PRO C 119 45.306 96.892, 3.119 1.00 27.70
ATOM 4471 CB PRO C 119 46.105 96.455 4.359 1.00 31.28
~0

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4472 CG PRO C 119 47.319 97.367 4.403 1.00 29.88 6
ATOM 4473 C PRO C 119 44.068 97.752 3.434 1.00 27.37 6
ATOM 4474 O PRO C 119 44.170 98.906 3.855 1.00 26.16 8
ATOM 4475 N LEU C 120 42.894 97.197 3.194 1.00 46.27 7
ATOM 4476 CA LEU C 120 41.687 97.952 3.437 1.00 47.57 6
ATOM 4477 CB LEU C 120 40.616 97.551 2.443 1.00 61.98 6
ATOM 4478 CG LEU C 120 40.905 98.073 1.042 1.00 62.57 6
ATOM 4479 CD1 LEU C 120 40.198 97.222 0.015 1.00 64.23 6
ATOM 4480 CD2 LEU C 120 40.470 99.524 0.947 1.00 61.87 6
ATOM 4481 C LEU C 120 41.197 97.747 4.845 1.00 48.67 6
ATOM 4482 0 LEU C 120 41.637 96.827 5.534 1.00 48.90 8
ATOM 4483 N MET C 121 40.292 98.624 5.271 1.00 42.57 7
ATOM 4484 CA MET C 121 39.718 98.581 6.614 1.00 43.86 6
ATOM 4485 CB MET C 121 39.714 100.003 7.225 1.00 46.78 6
ATOM 4486 CG MET C 121 39.464 100.086 8.744 1.00 49.57 6
ATOM 4487 SD MET C 121 38.990 101.748 9.383 1.00 52.53 16
ATOM 4488 CE MET C 121 40.558 102.447 9.830 1.00 54.56 6
ATOM 4489 C MET C 121 38.292 98.046 6.483 1.00 43.88 6
ATOM 4490 0 MET C 121 37.494 98.558 5.705 1.00 44.11 8
ATOM 4491 N THR C 122 37.973 97.001 7.229 1.00 29.41 7
ATOM 4492 CA THR C 122 36.638 96.441 7.171 1.00 30.53 6
ATOM 4493 CB THR C 122 36.553 95.186 7.993 1.00 59.28 6
ATOM 4494 OG1 THR C 122 37.695 94.376 7.717 1.00 59.40 8
ATOM 4495 CG2 THR C 122 35.290 94.423 7.643 1.00 61.33 6
ATOM 4496 C THR C 122 35.622 97.438 7.723 1.00 31.12 6.
ATOM 4497 0 THR C 122 35.999 98.406 8.386 1.00 31.55 8
ATOM 4498 N GLU C 123 34.338 97.198 7.456 1.00 49.45 7
ATOM 4499 CA GLU C 123 33.262 98.074 7.934 1.00 49.84 6
ATOM 4500 CB GLU C 123 31.908 97.434 7.631 1.00 90.22 6
ATOM 4501 CG GLU C 123 30.716 98.334 7.902 1.00 92.04 6
ATOM 4502 CD GLU C 123 29.398 97.686 7.518 1.00 92.73 6
ATOM 4503 OE1 GLU C 123 28.619 97.333 8.433 1.00 93.55 8
ATOM 4504 OE2 GLU C 123 29.150 97.523 6.301 1.00 93.00 8
ATOM 4505 C GLU C 123 33.417 98.265 9.439 1.00 49.89 6
ATOM 4506 O GLU C 123 33.117 99.326 9.995 1.00 49.12 8
ATOM 4507 N ASP C 124 33.898 97.193 10.063 1.00 52.75 7
ATOM 4508 CA ASP C 124 34.159 97.086 11.496 1.00 52.67 6
ATOM 4509 CB ASP C 124 34.775 95.7.08 11.784 1.00 61.98 6
ATOM 4510 CG ASP C 124 34.826 95.382 13.260 1.00 64.87 6
ATOM 4511 OD1 ASP C 124 34.951 96.323 14.071 1.00 67.98 8
ATOM 4512 OD2 ASP C 124 34.752 94.182 13.612 1.00 66.35 8
ATOM 4513 C ASP C 124 35.116 98.183 11.968 1.00 50.94 6
ATOM 4514 O ASP C 124 35.043 98.633 13.118 1.00 50.05 8
ATOM 4515 N GLY C 125 36.025 98.590 11.083 1.00 62.59 7
ATOM 4516 CA GLY C 125 36.986 99.628 11.420 1.00 61.39 6
ATOM 4517 C GLY C 125 38.375 99.110 11.745 1.00 59.43 6
ATOM 4518 O GLY C 125 39.121 99.708 12.520 1.00 59.88 8
ATOM 4519 N SER C 126 38.733 97.995 11.133 1.00 77.36 7
ATOM 4520 CA SER C 126 40.030 97.394 11.372 1.00 73.95 6
ATOM 4521 CB SER C 126 39.883 96.359 12.492 1.00 13.87 6
ATOM 4522 OG SER C 126 38.551 95.856 12.538 1.00 13.87 8
ATOM 4523 C SER C 126 40.512 96.749 10.072 1.00 73.71 6
ATOM 4524 0 SER C 126 39.938 96.992. 9.008 1.00 75.22 8
ATOM 4525 N PHE C 127 41.570 95.948 10.138 1.00 28.88 7
ATOM 4526 CA PHE C 127 42.044 95.290 8.937 1.00 27.11 6
ATOM 4527 CB PHE C 127 43.339 95.927 8.455 1.00 51.61 6
81

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ATOM 4528 CG PHE C 127 43.347 97.420 8.516 1.00 53.45
6
ATOM 4529 CD1 PHE C 127 43.426 98.080 9.733 1.00 55.26
6
ATOM 4530 CD2 PHE C 127 43.328 98.174 7.355 1.00 54.19
6
ATOM 4531 CE1 PHE C 127 43.498 99.471 9.792 1.00 55.29
6
ATOM 4532 CE2 PHE C 127 43.398 99.566 7.404 1.00 55.13
6
ATOM 4533 CZ PHE C 127. 43.483 100.2138.624 1.00 55.33
6
ATOM 4534 C PHE C 127 42.291 93.806 9.193 1.00 26.15
6
ATOM 4535 0 PHE C 127 42.443 93.387 10.348 1.00 26.14
8
ATOM 4536 N ILE C 128 42.298 93.020 8.113 1.00 29.00
7
ATOM 4537 CA ILE C 128 42.588 91.583 8.164 1.00 27.66
6
ATOM 4538 CB ILE C 128 41.754 90.738 7.214 1.00 15.94
6
ATOM 4539 CG2 ILE C 128 42.249 89.318 7.278 1.00 14.69
6
ATOM 4540 CG1 ILE C 128 40.274 90.828 7.556 1.00 17.08
6
ATOM 4541 CD1 ILE C 128 39.693 92.171 7.310 1.00 20.48
6
ATOM 4542 C ILE C 128 43.968 91.516 7.566 1.00 28.11
6
ATOM 4543 O ILE C 128 44.150 91.905 6.414 1.00 29.53
8
ATOM 4544 N ILE C 129 44.940 91.023 8.317 1.00 53.67
7
ATOM 4545 CA ILE C 129 46.284 90.964 7.780 1.00 52.88
6
ATOM 4546 CB ILE C 129 47.187 91.997 8.468 1.00 15.23
6
ATOM 4547 CG2 ILE C 129 48.547 91.971 7.843 1.00 26.20
6
ATOM 4548 CG1 ILE C 129 46.553 93.385 8.329 1.00 13.87
6
ATOM 4549 CD1 ILE C 129 47.506 94.531 8.013 1.00 14.82
6
ATOM 4550 C ILE C 129 46.917 89.588 7.843 1.00 52.95
6
ATOM 4551 O ILE C 129 47.298 89.107 8.906 1.00 52.12
8
ATOM 4552 N ASN C 130 47.023 88.967 6.676 1.00 33.54
7
ATOM 4553 CA ASN C 130 47.596 87.641 6.540 1.00 34.43
6
ATOM 4554 CB ASN C 130 48.944 87.552 7.254 1.00 65.84
6
ATOM 4555 CG ASN C 130 49.953 88.539 6.706 1.00 66.87
6
ATOM 4556 OD1 ASN C 130 50.326 88.488 5.531 1.00 66.73
8
ATOM 4557 ND2 ASN C 130 50.396 89.453 7.557 1.00 66.85
7
ATOM 4558 C ASN C 130 46.640 86.613 7.098 1.00 34.18
6
ATOM 4559 O ASN C 130 47.036 85.501 7.438 1.00 35.03
8
ATOM 4560 N GLY C 131 45.373 86.992 7.191 1.00 27.94
7
ATOM 4561 CA GLY C 131 44.376 86.071 7.698 1.00 26.19
6
ATOM 4562 C GLY C 131 43.759 86.491 9.008 1.00 24.96
6
ATOM 4563 0 GLY C 131 42.551 86.411 9.164 1.00 23.55
8
ATOM 4564 N ALA C 132 44.595 86.938 9.938 1.00 40.17
7
ATOM 4565 CA ALA C 132 44.150 87.381 11.256 1.00 40.65
6
ATOM 4566 CB ALA C 132 45.280 87.214 12.257 1.00 77.38
6
ATOM 4567 C ALA C 132 43.641 88.832 11.283 1.00 40.65
6
ATOM 4568 O ALA C 132 43.964 89.640 10.407 1.00 41.21
8
ATOM 4569 N ASP C 133 42.843 89.149 12.302 1.00 15.55
7
ATOM 4570 CA ASP C 133 42.277 90.478 12.448 1.00 14.38
6
ATOM 4571 CB ASP C 133 40.892 90.404 13.073 1.00 64.17
6
ATOM 4572 CG ASP C 133 39.938 89.573 12.266 1.00 67.59
6
ATOM 4573 OD1 ASP C 133 39.457 90.068 11.226 1.00 70.82
8
ATOM 4574 OD2 ASP C 133 39.675 88.420 12.673 1.00 69.26
8
ATOM 4575 C ASP C 133 43.149 91.312 13.334 1.00 13.87
6
ATOM 4576 O ASP C 133 43.591 90.867 14.393 1.00 13.87
8
ATOM 4577 N ARG C 134 43.386 92.540 12.901 1.00 31.25
7
ATOM 4578 CA ARG C 134 44.204 93.457 13.677 1.00 30.63
6
ATOM 4579 CB ARG C 134 45.627 93.572 13.118 1.00 47.57
6
ATOM 4580 CG ARG C 134 46.194 92.313 12.479 1.00 51.45
6
ATOM 4581 CD ARG C 134 47.698 92.335 12.518 1.00 52.73
6
ATOM 4582 NE ARG C 134 48.169 91.668 13.716 1.00 54.72
7
ATOM 4583 CZ ARG C 134 49.360 91.878 14.247 1.00 57.60
6
82

CA 02440409 2003-09-09
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ATOM 4584 NH1 ARG C 134 50.173 92.749 13.668 1.00 58.08
ATOM 4585 NH2 ARG C 134 49.741 91.211 15.332 1.00 60.44
ATOM 4586 C ARG C 134 43.570 94.828 13.643 1.00 28.37
ATOM 4587 O ARG C 134 42.817 95.160 12.719 1.00 28.20
ATOM 4588 N VAL C 135 43.906 95.621 14.656 1.00 23.37
ATOM 4589 CA VAL C 135 43.402 96.976 14.800 1.00 20.65
ATOM 4590 CB VAL C 135 42.484 97.063 16.037 1.00 13.87
ATOM 4591 CG1 VAL C 135 41.236 97.848 15.700 1.00 13.87
ATOM 4592 CG2 VAL C 135 42.127 95.653 16.528 1.00 13.87
ATOM 4593 C VAL C 135 44.584 97.953 14.953 1.00 20.23
ATOM 4594 O VAL C 135 45.553 97.664 15.641 1.00 19.42
ATOM 4595 N ILE C 136 44.509 99.099 14.294 1.00 17.38
ATOM 4596 CA ILE C 136 45.566 100.09514.392 1.00 19.94
ATOM 4597 CB ILE C 136 45.684 100.89413.088 1.00 37.79
ATOM 4598 CG2 ILE C 136 46.695 102.03013.252 1.00 39.14
ATOM 4599 CG1 ILE C 136 46.090 99.953 11.957 1.00 39.94
ATOM 4600 CD1 ILE C 136 47.387 99.226 12.220 1.00 42.63
ATOM 4601 C ILE C 136 45.308 101.07115.548 1.00 20.26
ATOM 4602 O ILE C 136 44.535 102.02015.407 1.00 18.99
ATOM 4603 N VAL C 137 45.964 100.82416.682 1.00 25.21
ATOM 4604 CA VAL C 137 45.827 101.64617.888 1.00 25.11
ATOM 4605 CB VAL C 137 46.507 100.98519.088 1.00 16.86
ATOM 4606 CG1 VAL C 137 46.279 101.80420.338 1.00 15.96
ATOM 4607 CG2 VAL C 137 45.983 99.583 19.255 1.00 16.90
ATOM 4608 C VAL C 137 46.454 103.00317.684 1.00 25.80
ATOM 4609 O VAL C 137 47.591 103.11117.244 1.00 26.80
ATOM 4610 N SER C 138 45.703 104.03318.046 1.00 16.42
ATOM 4611 CA SER C 138 46.108 105.42617.881 1.00 18.38
ATOM 4612 CB SER C 138 44.825 106.23317.718 1.00 28.33
ATOM 4613 OG SER C 138 43.729 105.45918.193 1.00 25.47
ATOM 4614 C SER C 138 46.982 106.00718.998 1.00 20.62
ATOM 4615 0 SER C 138 46.548 106.09220.145 1.00 20.75
ATOM 4616 N GLN C 139 48.199 106.43318.665 1.00 29.81
ATOM 4617 CA GLN C 139 49.080 106.97019.698 1.00 33.75
ATOM 4618 CB GLN C 139 50.535 106.57619.443 1.00 57.86
'ATOM4619 CG GLN C 139 51.270 107.37718.388 1.00 59.42
ATOM 4620 CD GLN C 139 52.765 107.07918.396 1.00 61.08
ATOM 4621 OE1 GLN C 139 53.183 105.92518.295 1.00 60.50
ATOM 4622 NE2 GLN C 139 53.574 108.12218.515 1.00 62.00
ATOM 4623 C GLN C 139 48.999 108.46819.826 1.00 36.24
ATOM 4624 O GLN C 139 48.320 109.10719.040 1.00 37.91
ATOM 4625 N ILE C 140 49.678 109.02320.831 1.00 69.32
ATOM 4626 CA ILE C 140 49.696 110.47221.049 1.00 71.94
ATOM 4627 CB ILE C 140 48.751 110.92122.203 1.00 32.52
ATOM 4628 CG2 ILE C 140 47.328 110.63221.853 1.00 32.85
ATOM 4629 CG1 ILE C 140 49.099 110.21123.502 1.00 31.'17
ATOM 4630 CD1 ILE C 140 48.294 110.72024.656 1.00 28.81
ATOM 4631 C ILE C 140 51.088 111.00321.379 1.00 74.89
ATOM 4632 0 ILE C 140 51.830 110.39222.151 1.00 76.12
ATOM 4633 N HIS C 141 51.437 112.14420.785 1.00 41.40
ATOM 4634 CA HIS C 141 52.731 112.79221.026 1.00 44.28
ATOM 4635 CB HIS C 141 53.683 112.58519.846 1.00100.71
ATOM 4636 CG HIS C 141 53.140 113.08018.541 1.00103.58
ATOM 4637 CD2 HIS C 141 53.213 114.29617.949 1.00105.25
ATOM 4638 ND1 HIS C 141 52.387 112.29017.700 1.00104.95
ATOM 4639 CE1 HIS C 141 52.019 112.99716.647 1.00105.35
83

CA 02440409 2003-09-09
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ATOM 4640 NE2 HIS C 141 52.507 114.218 16.773 1.00105.39 7
ATOM 4641 C HIS C 141 52.497 114.284 21.222 1.00 45.08 6
ATOM 4642 O HIS C 141 51.361 114.753 21.199 1.00 45.45 8
ATOM 4643 N ARG C 142 53.560 115.046 21.404 1.00 45.25 7
ATOM 4644 CA ARG C 142 53.350 116.465 21.603 1.00 46.96 6
ATOM 4645 CB ARG C 142 54'.602 117.101 22.211 1.00 87.26 6
ATOM 4646 CG ARG C 242 54.293 218.353 23.024 1.00 88.28 6
ATOM 4647 CD ARG C 142 54.803 118.265 24.456 1.00 88.37 6
ATOM 4648 NE ARG C 142 56.001 119.070 24.671 1.00 85.96 7
ATOM 4649 CZ ARG C 142 57.179 118.808 24.125 1.00 84.10 6
ATOM 4650 NH1 ARG C 142 57.320 117.756 23.334 1.00 81.90 7
ATOM 4651 NH2 ARG C 142 58.209 119.606 24.361 1.00 83.03 7
ATOM 4652 C ARG C 142 52.988 117.131 20.276 1.00 47.16 6
ATOM 4653 O ARG C 142 53.758 117.043 19.325 1.00 46.66 8
ATOM 4654 N SER C 143 51.825 117.788 20.207 1.00 24.06 7
ATOM 4655 CA SER C 143 51.377 118.446 18.966 1.00 24.67 6
ATOM 4656 CB SER C 143 50.112 119.266 19.219 1.00137.30 6
ATOM 4657 OG SER C 143 50.380 120.379 20.052 1.00140.68 8
ATOM 4658 C SER C 143 52.455 119.347 18.388 1.00 23.88 6
ATOM 4659 0 SER C 143 52.917 120.257 19.040 1.00 23.01 8
ATOM 4660 N PRO C 144 52.861 119.102 17.145 1.00 54.84 7
ATOM 4661 CD PRO C 144 52.222 118.197 16.178 1.00 78.94 6
ATOM 4662 CA PRO C 144 53.898 119.904 16.500 1.00 56.24 6
ATOM 4663 CB PRO C 144 53.619 119.686 15.025 1.00 79.24 6
ATOM 4664 CG PRO C 144 53.168 11$.272 15.006 1.00 79.94 6
ATOM 4665 C PRO C 144 53.867 121.377 16.886 1.00 57.78 6
ATOM 4666 O PRO C 144 52.795 121.989 16.943 1.00 58.39 8
ATOM 4667 N GLY C 145 55.043 121.943 17.150 1.00 56.07 7
ATOM 4668 CA GLY C 145 55.116 123.345 17.516 1.00 57.15 6
ATOM 4669 C GLY C 145 56.431 123.783 18.132 1.00 58.03 6
ATOM 4670 O GLY C 145 57.505 123.559 17.576 1.00 58.65 8
ATOM 4671 N VAL C 146 56.328 124.409 19.301 1.00 70.55 7
ATOM 4672 CA VAL C 146 57.469 124.934 20.054 1.00 71.73 6
ATOM 4673 CB VAL C 146 58.084 126.164 19.321 1.00 86.38 6
ATOM 4674 CG1 VAL C 146 58.807 127.069 20.304 1.00 87.82 6
ATOM 4675 CG2 VAL C 146 59.045 125.699 18.241 1.00 87.33 6
ATOM 4676 C VAL C 146 56.920 125.377 21.411 1.00 71.72 6
ATOM 4677 O VAL C 146 55.961 126.139 21.457 1.00 71.68 8
ATOM 4678 N TYR C 147 57.503 124.911 22.512 1.00117.29 7
ATOM 4679 CA TYR C 147 56.984 125.318 23.814 1.00117.99 6
ATOM 4680 CB TYR C 147 56.107 124.229 24.423 1.00 56.96 6
ATOM 4681 CG TYR C 147 55.592 123.190 23.463 1.00 55.38 6
ATOM 4682 CD1 TYR C 147 56.452 122.268 22.880 1.00 54.18 6
ATOM 4683 CE1 TYR C 147 55.963 121.274 22.056 1.00 53.00 6
ATOM 4684 CD2 TYR C 147 54.230 123.090 23.186 1.00 55.16 6
ATOM 4685 CE2 TYR C 147 53.739 122.106 22.365 1.00 53.62 6
ATOM 4686 CZ TYR C 147 54.604 121.201 21.807 1.00 52.84 6
ATOM 4687 OH TYR C 147 54.104 120.196 21.022 1.00 53.09 8
ATOM 4688 C TYR C 147 58.020 125.703 24.856 1.00119.45 6
ATOM 4689 O TYR C 147 57.672 126.267 25.890 1.00121.23 8
ATOM 4690 N PHE C 148 59.282 125.385 24.599 1.00127.73 7
ATOM 4691 CA PHE C 148 60.356 125.701 25.534 1.00127.40 6
ATOM 4692 CB PHE C 148 60.178 127.120 26.072 1.00 72.62 6
ATOM 4693 CG PHE C 148 60.496 228.270 25.051 1.00 72.89 6
ATOM 4694 CD1 PHE C 148 59.873 128.150 23.813 1.00 72.38 6
ATOM 4695 CD2 PHE C 148 61.487 129.109 25.281 1.00 73.32 6
84

CA 02440409 2003-09-09
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ATOM 4696 CE1 PHE C 148 60.239 129.032 22.820 1.00 72.68
6
ATOM 4697 CE2 PHE C 148 61.859 129.995 24.290 1.00 73.89
6
ATOM 4698 CZ PHE C 148 61.234 129.956 23.056 1.00 73.37
6
.ATOM 4699 C PHE C 148 60.465 124.672 26.653 1.00127.51
6
ATOM 4700 0 PHE C 148 60.867 123.547 26.365 1.00129.'30
8
ATOM 4701 N THR C 149 60.129 125.027 27.899 1.00 41.88
7
ATOM 4702 CA THR C 149 60.218 124.066 29.020 1.00 40.35
6
ATOM 4703 CB THR C 149 60.313 122.622 28.508 1.00 45.51
6
ATOM 4704 OG1 THR C 149 59.144 122.319 27.751 1.00 45.70
8
ATOM 4705 CG2 THR C 149 60.481 121.638 29.638 1.00 45.67
6
ATOM 4706 C THR C 149 61.430 124.263 29.932 1.00 39.60
6
ATOM 4707 0 THR .C149 62.506 124.636 29.468 1.00 39.78
8
ATOM 4708 N PRO C 150 61.274 123.994 31.244 1.00 43.39
7
ATOM 4709 CD PRO C 150 60.009 123.690 31.934 1.00 74.13
6
ATOM 4710 CA PRO C 150 62.356 124.134 32.226 1.00 42.51
6
ATOM 4711 CB PRO C 150 61.624 124.041 33.559 1.00 73.28
6
ATOM 4712 CG PRO C 150 60.504 123.118 33.242 1.00 74.47
6
ATOM 4713 C PRO C 150 63.445 123.072 32.100 1.00 41.93
6
ATOM 4714 0 PRO C 150 63.175 121.882 32.204 1.00 41.14
8
ATOM 4715 N ASP C 151 64.675 123.523 31.888 1.00 60.62
7
ATOM 4716 CA ASP C 151 65.818 122.636 31.750 1.00 61.64
6
ATOM 4717 CB ASP C 151 67.095 123.459 31.661 1.00 82.47
6
ATOM 4718 CG ASP C 151 68.284 122.636 31.246 1.00 83.64
6
ATOM 4719 OD1 ASP C 151 68.498 121.571 31.861 1.00 85.08
8
ATOM 4720 OD2 ASP C 151 69.005 123.054 30.310 1.00 83.86
8
ATOM 4721 C ASP C 151 65.884 121.736 32.970 1.00 62.64
6
ATOM 4722 0 ASP C 151 66.220 122.190 34.060 1.00 62.88
8
ATOM 4723 N PRO C 152 65.584 120.441 32.801 1.00 53.51
7
ATOM 4724 CD PRO C 152 65.413 119.745 31.515 1.00152.73
6
ATOM 4725 CA PRO C 152 65.605 119.475 33.907 1.00 53.78
6
ATOM 4726 CB PRO C 152 65.331 118.146 33.205 1.00153.68
6
ATOM 4727 CG PRO C 152 65.910 118.364 31.837 1.00153.85
6
ATOM 4728 C PRO C 152 66.890 119.445 34.742 1.00 53.62
6
ATOM 4729 0 PRO C 152 66.836 119.280 35.965 1.00 52.96
8
ATOM 4730 N ALA C 153 68.034 119.590 34.077 1.00212.64
7
ATOM 4731 CA ALA C 153 69.326 119.588 34.754 1.00113.39
6
ATOM 4732 CB ALA C 153 70.404 120.125 33.817 1.00 30.18
6
ATOM 4733 C ALA C 153 69.232 120.458 36.002 1.00113.68
6
ATOM 4734 O ALA C 153 68.842 119.992 37.075 1.00113.62
8
ATOM 4735 N ARG C 154 69.600 121.724 35.854 1.00 60.59
7
ATOM 4736 CA ARG C 154 69.532 122.675 36.953 1.00 60.51
6
ATOM 4737 CB ARG C 154 70.924 122.944 37.515 1.00102.11
6
ATOM 4738 CG ARG C 154 71.529 121.762 38.255 1.00103.38
6
ATOM 4739 CD ARG C 154 70.707 121.387 39.481 1.00105.00
6
ATOM 4740 NE ARG C 154 71.391 120.388 40.300 1.00106.91
7
ATOM 4741 CZ ARG C 154 70.934 119.922 41.459 1.00107.11
6
ATOM 4742 NH2 ARG C 154 69.782 12 0.36242:946 1.00107.07
7
ATOM 4743 NH2 ARG C 154 71.634 119.018 42.133 1.00106.92
7
ATOM 4744 C ARG C 154 68.934 123.945 36.367 1.00 60.26
6
ATOM 4745 O ARG C 154 69.385 124.409 35.317 1.00 60.90
8
ATOM 4746 N PRO C 155 67.921 124.525 37.046 1.00123.78
7
ATOM 4747 CD PRO C 155 67.773 124.324 38.492 1.00 72.44
6
ATOM 4748 CA PRO C 155 67.197 125.742 36.654 1.00123.95
6
ATOM 4749 CB PRO C 155 67.106 126.545 37.964 1.00 72.35
6
ATOM 4750 CG PRO C 155 67.916 125.733 38.984 1.00 72.06
6
ATOM 4751 C PRO C 155 67.791 126.542 35.500 1.00123.67
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4752 0 PRO C 155 68.825 127.20035.637 1.00123.37
8
ATOM 4753 N GLY C 156 67.118 126.47434.356 1.00 79.22
7
ATOM 4754 CA GLY C 156 67.586 127.18633.188 1.00 78.18
6
ATOM 4755 C GLY C 156 66.950 126.67431.918 1.00 78.05
6
ATOM 4756 0 GLY C 156 67.636 126.09432.082 1.00 78.51
8
ATOM 4757 N ARG C 157 65.645 126.89531.778 1.00 53.95
7
ATOM 4758 CA ARG C 157 64.886 126.47730.597 1.00 53.98
6
ATOM 4759 CB ARG C 157 63.700 127.41830.360 1.00 62.64
6
ATOM 4760 CG ARG C 157 63.671 128.65831.234 1.00 62.58
6
ATOM 4761 CD ARG C 157 62.903 128.38332.518 1.00 62.90
6
ATOM 4762 NE ARG C 157 62.815 129.55833.379 1.00 62.14
7
ATOM 4763 CZ ARG C 157 62.214 129.57234.565 1.00 61.89
6
ATOM 4764 NH1 ARG C 157 61.643 128.47035.040 1.00 61.22
7
ATOM 4765 NH2 ARG C 157 62.191 130.68835.282 1.00 61.30
7
ATOM 4766 C ARG C 157 65.648 126.36229.269 1.00 54.44
6
ATOM 4767 0 ARG C 157 66.756 126.89329.096 1.00 54.30
8
ATOM 4768 N TYR C 158 65.030 125.65528.327 1.00121.76
7
ATOM 4769 CA TYR C 158 65.591 125.47326.994 1.00122.22
6
ATOM 4770 CB TYR C 158 66.222 124.08826.830 1.00123.23
6
ATOM 4771 CG TYR C 158 65.461 122.96927.486 1.00124.02
6.
ATOM 4772 CD1 TYR C 158 64.074 122.99827.575 1.00124.12
6
ATOM 4773 CE1 TYR C 158 63.373 121.96628.181 1.00125.39
6
ATOM 4774 CD2 TYR C 158 66.133 121.87428.016 1.00125.08
6
ATOM 4775 CE2 TYR C 158 65.443 120.83328.621 1.00126.19
6
ATOM 4776 CZ TYR C 158 64.059 120.88528.703 1.00126.37
6
ATOM 4777 OH TYR C 158 63.365 119.86029.310 1.00126.79
8
ATOM 4778 C TYR C 158 64.490 125.64925.967 1.00121.49
6
ATOM 4779 O TYR C 158 63.373 126.04126.300 1.00121.07
8
ATOM 4780 N ILE C 159 64.807 125.35624.714 1.00 29.94
7
ATOM 4781 CA ILE C 159 63.836 125.50023.648 1.00 29.68
6
ATOM 4782 CB ILE C 159 64.154 126.71522.764 1.00 68.63
6
ATOM 4783 CG2 ILE C 159 62.944 127.06621.902 1.00 68.71
6
ATOM 4784 CG1 ILE C 159 64.518 127.90823.642 1.00 69.14
6
ATOM 4785 CD1 ILE C 159 64.982 129.10522.855 1.00 70.48
6
ATOM 4786 C ILE C 159 63.781 124.26922.757 1.00 29.29
6
ATOM 4787 0 ILE C 159 64.799 123.80422.231 1.00 28.38
8
ATOM 4788 N ALA C 160 62.574 123.74622.596 1.00 62.13
7
ATOM 4789 CA ALA C 160 62.358 122.58421.765 1.00 62.42
6
ATOM 4790 CB ALA C 160 61.670 121.49222.576 1.00 13.87
6
ATOM 4791 C ALA C 160 61.495 123.01420.589 1.00 63.06
6
ATOM 4792 O ALA C 160 60.536 123.76720.749 1.00 63.00
8
ATOM 4793 N SER C 161 61.847 122.55419.399 1.00 52.02
7
ATOM 4794 CA SER C 161 61.082 122.89718.215 1.00 53.53
6
ATOM 4795 CB SER C 161 61.958 123.71317.264 1.00 35.62
6
ATOM 4796 OG SER C 161 61.206 124.20916.171 1.00 33.38
8
ATOM 4797 C SER C 161 60.604 121.60317.542 1.00 55.90
6
ATOM 4798 0 SER C 161 61.330 121.00916.744 1.00 56.86
8
ATOM 4799 N ILE C 162 59.389 121.16717.876 1.00 66.20
7
ATOM 4800 CA ILE C 162 58.800 119.94217.321 1.00 69.04
6
ATOM 4801 CB ILE C 162 57.438 119.63118.001 1.00110.64
6
ATOM 4802 CG2 ILE C 162 56.653 118.60417.196 1.00110.49
6
ATOM 4803 CG1 ILE C 162 57.666 119.07519.408 1.00112.67
6
ATOM 4804 CD1 ILE C 162 58.586 119.89220.279 1.00115.54
6
ATOM 4805 C ILE C 162 58.595 119.99715.802 1.00 70.21
6
ATOM 4806 O ILE C 162 58.684 121.04815.184 1.00 70.59
8
ATOM 4807 N ILE C 163 58.319 118.85115.205 1.00 55.46
7
86

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4808 CA ILE C 163 58.113 118.776 13.779 1.00 58.28 6
ATOM 4809 CB ILE C 163 59.297 118.055 13.117 1.00108.27 ~6
ATOM 4810 CG2 ILE C 163 59.951 118.969 12.104 1.00109.24 6
ATOM 4811 CG1 ILE C 163 60.352 .117.70514.166 1.00109.10 6
ATOM 4822 CD1 ILE C 263 59.941 116.607 15.220 1.00109.92 6
ATOM 4813 C ILE C 163 56.794 118.036 13'.537 1.00 60.36 6
ATOM 4814 0 ILE C 163 56.397 117.203 14.353 1.00 60.87 8
ATOM 4815 N PRO C 164 56.116 118.314 12.400 1.00170.37 7
ATOM 4816 CD PRO C 164 56.844 118.802 11.219 1.00206.16 6
ATOM 4817 CA PRO C 164 54.830 117.739 11.970 1.00172.36 6
ATOM 4818 CB PRO C 164 54.944 117.723 10.440 1.00206.05 6
ATOM 4819 CG PRO C 164 56.420 117.813 10.180 1.00206.20 6
ATOM 4820 C PRO C 164 ' 54.465116.377 12.542 1.00172.85 6
ATOM 4821 0 PRO C 164 53.383 116.198 13.099 1.00173.08 8
ATOM 4822 N LEU C 165 55.363 115.417 12.375 1.00 84.53 7
ATOM 4823 CA LEU C 165 55.172 114.069 12.893 1.00 86.24 6
ATOM 4824 CB LEU C 165 54.306 113.221 11.951 1.00183.33 6
ATOM 4825 CG LEU C 165 52.809 113.557 11.928 1.001$5.30 6
ATOM 4826 CD1 LEU C 165 52.093 122.712 10.891 1.00186.01 6
ATOM 4827 CD2 LEU C 165 52.213 113.320 13.302 1.00185.64 6
ATOM 4828 C LEU C 165 56.557 113.476 13.012 1.00 86.63 6
ATOM 4829 0 LEU C 165 57.412 113.713 12.158 1.00 86.89 8
ATOM 4830 N PRO C 166 56.799 112.698 14.075 1.00129.88 7
ATOM 4831 CD PRO C 166 55.740 112.149 14.942 1.00 63.26 6
ATOM 4832 CA PRO C 166 58.087 222.049 14.348 1.00130.73 6
ATOM 4833 CB PRO C 166 57.676 110.812 15.132 1.00 63.82 6
ATOM 4834 CG PRO C 166 56.530 111.347 15.955 1.00 63.96 6
ATOM 4835 C PRO C 166 58.897 111.725 13.091 1.00131.07 6
ATOM 4836 0 PRO C 166 59.954 112.310 12.855 1.00131.68 8
ATOM 4837 N LYS C 167 58.399 110.795 12.285 1.00154.09 7
ATOM 4838 CA LYS C 167 59.085 110.433 11.057 1.00154.27 6
ATOM 4839 CB LYS C 167 58.579 109.081 10.544 1.00100.00 6
ATOM 4840 CG LYS C 167 58.792 107.916 11.512 1.00101.09 6
ATOM 4841 CD LYS C 167 58.184 106.614 10.967 1.00101.62 6
ATOM 4842 CE LYS C 167 58.275 105.457 11.967 1.00101.62 6
ATOM 4843 NZ LYS C 167 57.599 104.220 11.477 1.00102.16 ~7
ATOM 4844 C LYS C 167 58.834 111.521 10.015 1.00153.74 6
ATOM 4845 0 LYS C 167 58.104 111.312 9.046 1.00154.66 8
ATOM 4846 N ARG C 168 59.438 112.687 10.237 1.00208.87 7
ATOM 4847 CA ARG C 168 59.309 113.829 9.335 1.00208.87 6
ATOM 4848 CB ARG C 168 57.868 113.965 8.836 1.00146.96 6
ATOM 4849 CG ARG C 168 57.609 115.305 8.191 1.00149.03 6
ATOM 4850 CD ARG C 168 56.342 115.325 7.376 1.00150.54 6
ATOM 4851 NE ARG C 168 56.202 116.607 6.692 1.00151.71 7
ATOM 4852 CZ ARG C 168 55.323 116.851 5.727 1.00153.30 6
.
ATOM 4853 NH1 ARG C 168 54.495 115.898 5.325 1.00155.10 7
ATOM 4854 NH2 ARG C 168 55.272 118.049 5.161 1.00153.41 7
ATOM 4855 C ARG C 168 59.736 115.157 9.967 1.00208.87 6
ATOM 4856 0 ARG C 168 59.030 115.699 10.818 1.00208.87 8
ATOM 4857 N GLY C 169 60.880 115.685 9.534 1.00101.34 7
ATOM 4858 CA GLY C 169 61.369 116.945 10.067 1.00 96.06 6
ATOM 4859 .C GLY C 169 62.264 116.774 11.280 1.00 92.86 6
ATOM 4860 0 GLY C 169 62.176 115.768 11.978 1.00 93.47 8
ATOM 4861 N PRO C 170 63.155 117.733 11.549 1.00 79.40 7
ATOM 4862 CD PRO C 170 63.788 118.589 10.539 1.00 58.08 6
ATOM 4863 CA PRO C 170 64.032 117.597 12.716 1.00 75.94 6
87

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4864 CB PRO C 270 65.343 118.234 12.253 1.00 55.31
6
ATOM 4865 CG PRO C 170 65.232 118.248 10.746 1.00 55.85
6
ATOM 4866 C PRO C 170 63.506 118.257 13.990 1.00 73.58
6
ATOM 4867 0 PRO C 170 62.975 119.372 13.972 1.00 73.71
8
ATOM 4868 N TRP C 171 63.667 117.536 15.091 1.00 62.07
7
ATOM 4869 CA TRP C 171 63.265 117.978 16.415 1.00 58.42
6
ATOM 4870 CB TRP C 171 62.937 116.743 17.265 1.00 73.02
6
ATOM 4871 CG TRP C 171 62.826 116.966 18.741 1.00 71.24
6
ATOM 4872 CD2 TRP C 171 61.630 116,935 19.519 1.00 70.57
6
ATOM 4873 CE2 TRP C 171 61.996 117.177 20.858 1.00 69.79
6
ATOM 4874 CE3 TRP C 171 60.282 116.726 19.214 1.00 69.74
6
ATOM 4875 CD1 TRP C 171 63.844 117.218 19.616 1.00 70.80
6
ATOM 4876 NE1 TRP C 171 63.353 117.345 20.891 1.00 70.14
7
ATOM 4877 CZ2 TRP C 171 61:063 117.216 21.886 1.00 68.62
6
ATOM 4878 CZ3 TRP C 171 59.360 116,762 20.236 1.00 68.21
6
ATOM 4879 CH2 TRP C 171 59.752 117.007 21.556 1.00 68.40
6
ATOM 4880 C TRP C 171 64.474 118.728 16.964 1.00 57.22
6
ATOM 4881 O TRP C 171 65.439 118,116 17.436 1.00 57.05
8
ATOM 4882 N ILE C 172 64.422 120.054 16.869 1.00 46.00
7
ATOM 4883 CA ILE C 172 65.502 120.922 17.330 1.00 43.86
6
ATOM 4884 CB ILE C 172 65.619 122,181 16.417 1.00 53.44
6
ATOM 4885 CG2 ILE C 172 66.528 123.230 17.047 1.00 52.58
6
ATOM 4886 CG1 ILE C 172 66.152 121,776 15.044 1.00 53.18
6
ATOM 4887 CD1 ILE C 172 66.485 122.952 14.143 1.00 53.90
6
ATOM 4888 C ILE C 172 65.339 121.382 18.777 1.00 43.80
6
ATOM 4889 O ILE C 172 64.232 121.420 19.312 1.00 43.38
8
ATOM 4890 N ASP C 173 66.456 121.716 29.410 1.00 27.91
7
ATOM 4891 CA ASP C 173 66.447 122.216 20.773 1.00 28.66
6
ATOM 4892 CB ASP C 173 66.740 121.095 21.765 1.00 75.82
6
ATOM 4893 CG ASP C 173 65.505 120.346 22.177 1.00 77.50
6
ATOM 4894 OD1 ASP C 173 64.542 120.998 22.616 1.00 79.36
8
ATOM 4895 OD2 ASP C 173 65.498 119.105 22.074 1.00 78.29
8
ATOM 4896 C ASP C 173 67.500 123.312 20.918 1.00 29.71
6
ATOM 4897 O ASP C 173 68.668 123.104 20.580 1.00 29.08
8
ATOM 4898 N LEU C 174 67.092 124.485 21.393 1.00 51.31
7
ATOM 4899 CA LEU C 174 68.032 125.587 21.590 1.00 52.02
6
ATOM 4900 CB LEU C 174 67.456 126.917 21.099 1.00 30.46
6
ATOM 4901 CG LEU C 174 66.724 127.041 19.762 1.00 27.68
6
ATOM 4902 CD1 LEU C 174 66.242 128.477 19.614 1.00 27.71
6
ATOM 4903 CD2 LEU C 174 67.632 126.655 18.604 1.00 27.25
6
ATOM 4904 C LEU C 174 68.232 125.674 23.089 1.00 53.81
6
ATOM 4905 O LEU C 174 67.301 126.000 23.820 1.00 52.87
8
ATOM 4906 N GLU C 175 69.440 125.393 23.554 1.00 91.87
7
ATOM 4907 CA GLU C 175 69.693 125.428 24.985 1.00 95.65
6
ATOM 4908 CB GLU C 175 70.099 124.031 25.465 1.00184.07
6
ATOM 4909 CG GLU C 175 70.397 123.942 26.955 1.00187.25
6
ATOM 4910 CD GLU C 175 70.953 122.590 27.358 1.00189.17
6
ATOM 4911 OE1 GLU C 175 72.020 122.204 26.832 1.00189.79
8
ATOM 4912 OE2 GLU C 175 70.325 121.912 28.200 1.00190.45
8
ATOM 4913 C GLU C 175 70.750 126.434 25.419 1.00 97.00
6
ATOM 4914 0 GLU C 175 71.828 126.502 24.831 1.00 97.41
8
ATOM 4925 N VAL C 176 70.425 127.220 26.445 1.00109.36
7
ATOM 4916 CA VAL C 176 71.362 128.192 27.008 1.00111.86
6
ATOM 4917 CB VAL C 176 70.631 129.383 27.649 1.00 99.51
6
ATOM 4918 CG1 VAL C 176 70.510 130.519 26.653 1.00 99.77
6
ATOM 4919 CG2 VAL C 176 69.251 128.941 28.118 1.00 99.99
6
8~

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4920 C VAL C 176 72.099 127.418 28.096 1.00113.46
6
ATOM 4921 0 VAL C 176 71.473 126.934 29.041 1.00113.99
8
ATOM 4922 N GLU C 177 73.418 127.301 27.963 1.00114.43
7
ATOM 4923 CA GLU C 177 74.213 126.533 28.916 1.00115.97
6
ATOM 4924 CB GLU C 177 74.934 125.409 28.174 1.00 69.53
6
ATOM 4925 CG GLU C 177 75.954 125.905 27.171 1.00 69.55
6
ATOM 4926 CD GLU C 177 75.801 125.258 25.812 1.00 70.88
6
ATOM 4927 OE1 GLU C 177 74.720 125.413 25.211 1.00 72.04
8
ATOM 4928 OE2 GLU C 177 76.757 124.598 25.341 1.00 72.21
8
ATOM 4929 C GLU C 177 75.235 127.319 29.726 1.00117.14
6
ATOM 4930 0 GLU C 177 75.312 128.547 29.652 1.00118.00
8
ATOM 4931 N ALA C 178 76.021 126.584 30.505 1.00 86.35
7
ATOM 4932 CA ALA C 178 77.059 127.180 31.330 1.00 86.34
6
ATOM 4933 CB ALA C 178 77.411 126.249 32.471 1.00 60.35
6
ATOM 4934 C ALA C 178 78.278 127.404 30.461 1.00 86.27
6
ATOM 4935 O ALA C 178 78.648 128.541 30.163 1.00 86.17
8
ATOM 4936 N SER C 179 78.888 126.297 30.051 1.00124.62
7
ATOM 4937 CA SER C 179 80.078 126.325 29.215 1.00124.26
6
ATOM 4938 CB SER C 179 81.081 125.283 29.720 1.00 83.67
, 6
ATOM 4939 OG SER C 179 80.429 124.068 30.043 1.00 83.11
8
ATOM 4940 C SER C 179 79.753 126.077 27.744 1.00124.15
6
ATOM 4941 0 SER C 179 79.622 124.932 27.308 1.00124.27
8
ATOM 4942 N GLY C 180 79.628 127.164 26.986 1.00190.01
7
ATOM 4943 CA GLY C 180 79.316 127.065 25.570 1.00188.94
6
ATOM 4944 C GLY C 180 78.424 128.207 25.118 1.00187.48
6
ATOM 4945 0 GLY C 180 78.811 129.018 24.277 1.00187.77
8
ATOM 4946 N VAL C 181 77.222 128.255 25.684 1.00101.50
7
ATOM 4947 CA VAL C 181 76.236 129.290 25.391 1.00 98.68
6
ATOM 4948 CB VAL C 181 76.881 130.682 25.309 1.00 85.33
6
ATOM 4949 CG1 VAL C 182 75.817 231.729 25.005 1.00 86.21
6
ATOM 4950 CG2 VAL C 181 77.590 130.999 26.616 1.00 85.64
6
ATOM 4951 C VAL C 181 75.447 129.066 24.115 1.00 97.12
6
ATOM 4952 O VAL C 181 75.970 129.208 23.016 1.00 98.03
8
ATOM 4953 N VAL C 182 74.174 128.728 24.283 1.00 49.17
7
ATOM 4954 CA VAL C 182 73.254 128.492 23.176 1.00 47.30
6
ATOM 4955 CB VAL C 182 72.965 129.801 22.412 1.00 88.90
6
ATOM 4956 CG1 VAL C 182 71.625 129.697 21.703 1.00 88.65
6
ATOM 4957 CG2 VAL C 182 72.958 130.978 23.380 1.00 89.47
6
ATOM 4958 C VAL C 182 73.693 127.414 22.184 1.00 45.25
6
ATOM 4959 0 VAL C 182 74.519 127.638 21.296 1.00 44.44
8
ATOM 4960 N THR C 183 73.108 126.235 22.351 1.00 54.04
7
ATOM 4961 CA THR C 183 73.398 125.098 21.499 1.00 52.78
6
ATOM 4962 CB THR C 183 73.811 123.873 22.351 1.00 57.05
6
ATOM 4963 OG1 THR C 183 74.944 124.217 23.156 1.00 57.23
8
ATOM 4964 CG2 THR C 183 74.183 122.689 21.460 1.00 56.91
6
ATOM 4965 C THR C 183 72.166 124.752 20.658 1.00 52.66
6
ATOM 4966 0 THR C 183 71.052 124.630 21.174 1.00 52.53
8
ATOM 4967 N MET C 184 72.382 124.621 19.354 1.00119.00
7
ATOM 4968 CA MET C 184 71.328 124.274 18.409 1.00118.73
6
ATOM 4969 CB MET C 184 71.469 125.124 17.139 1.00 40.44
6
ATOM 4970 CG MET C 184 70.168 125.682 16.555 1.00 36.98
6
ATOM 4971 SD MET C 184 69.194 124.596 15.491 1.00 33.84
16
ATOM 4972 CE MET C 184 69.676 125.144 13.911 1.00 34.18
6
ATOM 4973 C MET C 184 71.586 122.805 18.088 1.00120.26
~6
ATOM 4974 0 MET C 184 72.524 122.489 17.353 1.00121.69
8
ATOM 4975 N LYS C 185 70.780 121.908 18.651 1.00 65.73
7
89

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 4976 CA LYS C 185 70.981 120.48818.397 1.00 66.80
6
ATOM 4977 CB LYS C 185 71.015 119.71319.718 1.00112.77
6
ATOM 4978 CG LYS C 185 70.001 120.16920.737 1.00113.93
6
ATOM 4979 CD LYS C 185 70.298 119.57922.108 1.00114.95
6
ATOM 4980 CE LYS C 185 69.505 120.30623.185 1.00115.35
6
ATOM 4981 NZ LYS C 185 69.807 119.83124.558 1.00116.49
7
ATOM 4982 C LYS C 185 69.997 219.83417.435 1.00 67.41
6
ATOM 4983 0 LYS C 185 69.037 119.19317.848 1.00 67.37
8
ATOM 4984 N VAL C 186 70.244 119.99916.144 1.00 63.54
7
ATOM 4985 CA VAL C 186 69.397 119.38715.146 1.00 65.54
6
ATOM 4986 CB VAL C 186 69.662 119.98013.767 1.00 44.28
6
ATOM 4987 CG1 VAL C 186 68.491 119.70012.862 1.00 44.54
6
ATOM 4988 CG2 VAL C 186 69.889 121.47513.879 1.00 43.86
6
ATOM 4989 C VAL C 186 69.828 117.92815.183 1.00 68.57
6
ATOM 4990 0 VAL C 186 71.011 117.64515.366 1.00 70.21
8
ATOM 4991 N ASN C 187 68.875 117.01015.013 1.00 73.26
7
ATOM 4992 CA ASN C 187 69.146 115.57315.082 1.00 74.74
6
ATOM 4993 CB ASN C 187 69.404 114.96413.684 1.00 88.07
6
ATOM 4994 CG ASN C 187 70.368 115.77712.845 1.00 89.73
6
ATOM 4995 OD1 ASN C 187 71.370 116.27413.345 1.0 089.09
8
ATOM 4996 ND2 ASN C 187 70.076 115.89911.553 1.00 90.27
7
ATOM 4997 C ASN C 187 70.304 115.28116.035 1.00 75.73
6
ATOM 4998 0 ASN C 187 70.241 115.63217.212 1.00 76.73
8
ATOM 4999 N LYS C 188 71.362 114.65715.539 1.00 89.99
7
ATOM 5000 CA LYS C 188 72.506 114.33216.388 1.00 91.65
6
ATOM 5001 CB LYS C 288 72.981 212.90416.084 2.00132.01
6
ATOM 5002 CG LYS C 188 74.157 112.42316.935 1.00133.24
6
ATOM 5003 CD LYS C 188 74.602 111.01216.538 1.00134.93
6
ATOM 5004 CE LYS C 188 75.708 110.47917.449 1.00136.59
6
ATOM 5005 NZ LYS C 188 76.105 109.08417.104 1.00137.85
7
ATOM 5006 C LYS C 188 73.668 115.30916.212 1.00 91.77
6
ATOM 5007 0 LYS C 188 74.685 114.96415.611 1.00 91.94
8
ATOM 5008 N ALA C 189 73.531 116.52116.737 1.00100.63
7
ATOM 5009 CA ALA C 189 74.601 117.50016.599 1.00100.67
6
ATOM 5010 CB ALA C 189 74.513 118.17015.240 1.00100.93
6
ATOM 5011 C ALA C 189 74.581 118.55417.696 1.00100.94
6
ATOM 5012 0 ALA C 189 73.597 118.70018.416 1.00101.43
8
ATOM 5013 N LYS C 190 75.682 119.28517.817 1.00124.83
7
ATOM 5014 CA LYS C 190 75.814 120.34018.812 1.00123.69
6
ATOM 5015 CB LYS C 190 76.478 119.80720.083 1.00205.82
6
ATOM 5016 CG LYS C 190 76.921 120.90421.045 1.00208.87
6
ATOM 5017 CD LYS C 190 77.932 120.39822.065 1.00208.87
6
ATOM 5018 CE LYS C 190 78.485 121.54722.902 1.00208.87
6
ATOM 5019 NZ LYS C 190 79.555 121.11023.844 1.00208.87
7
ATOM 5020 C LYS C 190 76.690 121.42618.217 1.00122.07
6
ATOM 5021 0 LYS C 190 77.913 121.31918.244 1.00122.53
8
ATOM 5022 N PHE C 191 76.074 122.46717.673 1.00 55.07
7
ATOM 5023 CA PHE C 191 76.843 123.55317.083 1.00 52.94
6
ATOM 5024 CB PHE C 191 76.650 123.57015.568 2.00 54.01
6
ATOM 5025 CG PHE C 191 75.211 123.72015.134 1.00 53.37
6
ATOM 5026 CD1 PHE C 191 74.629 124.97615.004 1.00 53.59
6
ATOM 5027 CD2 PHE C 191 74.444 122.60614.836 1.00 53.02
6
ATOM 5028 CE1 PHE C 191 73.309 125.11414.580 1.00 52.15
6
ATOM 5029 CE2 PHE C 191 73.130 122.74314.415 1.00 52.41
6
ATOM 5030 CZ PHE C 191 72.567 123.99714.287 1.00 51.68
6
ATOM 5031 C PHE C 191 76.433 124.89027.663 1.00 51.32
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5032 0 PHE C 191 75.267 125.113 17.971 1.00 52.15
ATOM 5033 N PRO C 192 77.392 125.798 17.840 1.00 38.88
ATOM 5034 CD PRO C 192 78.832 125.719 17.536 1.00 182.54
ATOM 5035 CA PRO C 192 77.037 127.108 18.392 1.00 37.55
ATOM 5036 CB PRO C 292 78.294 127.929 18.138 1.00182.33
ATOM 5037 CG PRO C 192 79.385 126.904 18.294 1.00183.06
ATOM 5038 C PRO C 192 75.819 127.660 17.652 1.00 35.77
ATOM 5039 O PRO C 192 75.675 127.450 16.446 2.00 35.21
ATOM 5040 N LEU C 193 74.955 128.378 18.362 1.00 53.82
ATOM 5041 CA LEU C 193 73.741 128.907 17.743 1.00 51.81
ATOM 5042 CB LEU C 193 72.665 129.118 18.815 1.00143.95
ATOM 5043 CG LEU C 193 71.231 129.532 18.442 1.00143.18
ATOM 5044 CD1 LEU C 193 71.195 131.005 18.125 1.00143.77
ATOM 5045 CD2 LEU C 193 70.715 128.716 17.275 1.00143.01
ATOM 5046 C LEU C 193 73.911 130.171 16.917 1.00 50.43
ATOM 5047 0 LEU C 193 73.050 130.499 16.108 1.00 50.27
ATOM 5048 N VAL C 194 75.012 130.882 17.108 1.00107.98
ATOM 5049 CA VAL C 194 75.244 132.103 16.342 1.00107.22
ATOM 5050 CB VAL C 194 76.411 132.910 16.942 1.00 95.52
ATOM 5051 CG1 VAL C 194 76.099 134.399 16.883 1.00 94.22
ATOM 5052 CG2 VAL C 194 76.679 132.452 18.373 1.00 96.45
ATOM 5053 C VAL C 194 75.591 131.726 14.897 1.00105.88
ATOM 5054 O VAL C 194 75.346 132.489 13.958 2.00104.99
ATOM 5055 N LEU C 195 76.150 130.527 14.743 1.00 69.97
ATOM 5056 CA LEU C 195 76.571 129.995 13.454 1.00 67.27
ATOM 5057 CB LEU C 195 77.160 128.603 13.636 1.00 20.86
ATOM 5058 CG LEU C 195 77.876 128.058 12.409 1.00 19.45
ATOM 5059 CD1 LEU C 195 79.092 128.899 12.116 1.00 19.59
ATOM 5060 CD2 LEU C 195 78.288 126.624 12.668 1.00 19.54
ATOM 5061 C LEU C 195 75.461 129.928 12.427 1.00 66.47
ATOM 5062 O LEU C 195 75.594 130.463 11.332 1.00 66.59
ATOM 5063 N LEU C 196 74.370 129.252 12.770 1.00 54.77
ATOM 5064 CA LEU C 196 73.248 129.139 11.838 1.00 54.99
ATOM 5065 CB LEU C 196 72.178 128.167 12.353 1.00 44.73
ATOM 5066 CG LEU C 196 70.962 128.063 11.418 1.00 44.26
ATOM 5067 CD1 LEU C 196 71.049 126.752 10.631 1.00 43.98
ATOM 5068 CD2 LEU C 196 69.660 128.128 12.217 1.00 44.34
ATOM 5069 C LEU C 196 72.613 130.506 11.660 1.00 55.65
ATOM 5070 0 LEU C 196 72.135 130.849 10.571 1.00 55.48
ATOM 5071 N LEU C 197 72.604 131.275 12.744 1.00 52.15
ATOM 5072 CA LEU C 197 72.028 132.600 12.711 1.00 52.47
ATOM 5073 CB LEU C 197 72.115 133.264 14.077 1.00 16.86
ATOM 5074 CG LEU C 197 71.039 132.812 15.054 1.00 14.03
ATOM 5075 CD1 LEU C 197 71.142 133.690 16.257 1.00 14.44
ATOM 5076 CD2 LEU C 197 69.645 132.920 14.450 1.00 13.87
ATOM 5077 C LEU C 197 72.786 133.408 11.701 1.00 53.89
ATOM 5078 0 LEU C 197 72.196 134.052 10.834 1.00 54.03
ATOM 5079 N ARG C 198 74.106 133.357 11.809 1.00 85.09
ATOM 5080 CA ARG C 198 74.954 134.099 10.897 1.00 87.06
ATOM 5081 CB ARG C 198 76.405 133.646 11.025 1.00 58.75
ATOM 5082 CG ARG C 198 77.378 134.771 10.830 1.00 60.92
ATOM 5083 CD ARG C 198 76.964 135.625 9.662 1.00 63.11
ATOM 5084 NE ARG C 198 77.448 136.990 9.797 1.00 66.36
ATOM 5085 CZ ARG C 198 77.167 137.966 8.940 1.00 68.73
ATOM 5086 NH1 ARG C 198 76.403 137.727 7.883 1.00 69.89
ATOM 5087 NH2 ARG C 198 77.649 139.185 9.143 1.00 71.74
91

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5088 C ARG C 198 74.462 133.854 9.482 1.00 88.21 6
ATOM 5089 0 ARG C 198 74.060 134.787 8.788 1.00 88.90 8
ATOM 5090 N VAL C 199 74.491 132.591 9.067 1.00 65.98 7
ATOM 5092 CA VAL C 199 74.039 132.222 7.736 1.00 66.39 6
ATOM 5092 CB VAL C 199 73.850 130.711 7.578 1.00 50.32 6
ATOM 5093 CG1 VAL C 199 73.030 130.435 6.322 1.00 50.25 6
ATOM 5094 CG2 VAL C 199 75.209 130.015 7.489 1.00 50.24 6
ATOM 5095 C VAL C 199 72.702 132.865 7.504 1.00 67.56 6
ATOM 5096 0 VAL C 199 72.542 133.669 6.597 1.00 69.04 8
ATOM 5097 N LEU C 200 71.738 132.503 8.338 1.00 64.55 7
ATOM 5098 CA LEU C 200 70.397 133.053 8.217 1.00 66.04 6
ATOM 5099 CB LEU C 200 69.554 132.642 9.442 1.00 77.26 6
ATOM 5100 CG LEU C 200 69.452 131.141 9.778 1.00 76.80 6
ATOM 5101 CD1 LEU C 200 68.393 130.920 10.841 1.00 76.04 6
ATOM 5102 CD2 LEU C 200 69.101 130.350 8.541 1.00 76.36 6
ATOM 5103 C LEU C,200 70.447 134.588 8.055 1.00 66.96 6
ATOM 5104 O LEU C 200 69.625 135.178 7.338 1.00 67.17 8
ATOM 5105 N GLY C 201 71.423 135.226 8.704 1.00 58.18 7
'
ATOM 5106 CA GLY C 201 71.554 136.671 8.593 1.00 59.18 6
ATOM 5107 C GLY C 201 71.935 137.417 9.861 1.00 58.83 6
ATOM 5108 0 GLY C 201 72.865 138.220 9.857 1.00 58.22 8
ATOM 5109 N TYR C 202 71.197 137.172 10.939 1.00 74.72 7
ATOM 5110 CA TYR C 202 71.468 137.827 12.212 1.00 75.90 6
ATOM 5111 CB TYR C 202 70.633 137.206 13.331 1.00 67.76 6
ATOM 5112 CG TYR C 202 69.168 137.072 13.016 1.00 67.04 6
ATOM 5113 CD1 TYR C 202 68.442 135.991 13.487 1.00 66.74 6
ATOM 5114 CE1 TYR C 202 67.096 135.862 13.214 1.00 66.84 6
ATOM 5115 CD2 TYR C 202 68.504 138.029 12.257 1.00 67.11 6
ATOM 5116 CE2 TYR C 202 67.149 137.908 11.978 1.00 66.94 6
ATOM 5117 CZ TYR C 202 66.454 136.819 12.465 1.00 66.78 6
ATOM 5118 OH TYR C 202 65.109 136.678 12.225 1.00 67.58 8
ATOM 5119 C TYR C 202 72.927 137.597 12.517 1.00 77.83 6
ATOM 5120 0 TYR C 202 73.511 136.618 12.059 1.00 77.94 8
ATOM 5121 N ASP C 203 73.511 138.507 13.283 1.00101.22 7
ATOM 5122 CA ASP C 203 74.908 138.415 13.673 1.00103.17 6
ATOM 5123 CB ASP C 203 75.796 138.016 12.476 1.00128.66 6
ATOM 5124 CG ASP C 203 75.174 138.364 11.130 1.00128.95 6
ATOM 5125 OD1 ASP C 203 74.906 139.558 10.881 1.00131.25 8
ATOM 5126 OD2 ASP C 203 74.948 137.432 10.324 1.00126.78 8
ATOM 5127 C ASP C 203 75.408 139.719 14.283 1.00104.06 6
ATOM 5228'O ASP C 203 75.044 140.806 13.841 1.00103.50 8
ATOM 5129 N GLN C 204 76.235 139.580 15.314 1.00145.69 7
ATOM 5130 CA GLN C 204 76.832 140.703 16.027 1.00146.33 6
ATOM 5131 CB GLN C 204 78.328 140.777 15.705 1.00107.48 6
ATOM 5132 CG GLN C 204 79.041 141.959 16.342 1.00107.70 6
ATOM 5133 CD GLN C 204 78.924 141.968 17.855 1.00108.06 6
ATOM 5134 OE1 GLN C 204 77.823 141.894 18.405 1.00108.82 8
ATOM 5135 NE2 GLN C 204 80.061 142.066 18.536 1.00108.42 7
ATOM 5136 C GLN C 204 76.196 142.060 15.756 1.00146.63 6
ATOM 5137 0 GLN C 204 76.219 142.549 14.628 1.00147.43 8
ATOM 5138 N GLU C 205 75.641 142.669 16.799 1.00 47.05 7
ATOM 5139 CA GLU C 205 75.018 143.986 16.6$7 1.00 47..716
ATOM 5140 CB GLU C 205 75.975 144.989 16.023 1.00151.99 6
ATOM 5141 CG GLU C 205 77.376 145.056 16.634 1.00154.19 6
ATOM 5142 CD GLU C 205 77.376 145.395 18.116 1.00155.63 6
ATOM 5143 OE1 GLU C 205 76.805 146.440 18.493 1.00156.68 8
92

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5144 OE2 GLUC 205 77.958 144.620 18.905 1.00155.58 8
ATOM 5145 C GLUC 205 73.708 143.954 15.910 1.00 47.75 6
ATOM 5146 O GLUC 205 72.690 144.452 16.393 1.00 47.03 8
ATOM 5147 N THRC 206 73.725 143.385 14.706 1.00142.36 7
ATOM 5148 CA THR.C 206 72.500 143.295 13.913 1.00143.43 6
ATOM 5149 CB THRC 206 72.783 142.845 12.447 1.00106.22 6
ATOM 5150 OG1 THRC 206 71.713 143.282 11.600 1.00106.37 8
ATOM 5151 CG2 THRC 206 72.885 141.331 12.346 1.00105.31 6
ATOM 5152 C THRC 206 71.636 142.255 14.621 1.00143.64 6
ATOM 5153 0 THRC 206 70.408 142.250 14.505 1.00143.65 8
ATOM 5154 N LEUC 207 72.313 141.381 15.362 1.00 99.99 7
ATOM 5155 CA LEUC 207 71.677 140.325 16.139 1.00100.36 6
ATOM 5156 CB LEUC 207 72.574 139.084 16.179 1.00 70.78 6
ATOM 5157 CG LEUC 207 72.173 137.930 17.102 1.00 70.90 6
ATOM 5158 CD1 LEUC 207 70.832 137.349 16.672 1.00 70.57 6
ATOM 5159 CD2 LEUC 207 73.257 136.865 17.058 1.00 71.36 6
ATOM 5160 C LEUC 207 71.510 140.866 17.548 1.00100.14 6
ATOM 5161 0 LEUC 207 70.521 141.525 17.868 1.00100.30 8
ATOM 5162 N VALC 208 72.499 140.584 18.384 1.00 68.98 7
ATOM 5163 CA VALC 208 72.482 141.050 19.756 1.00 69.54 6
ATOM 5164 CB VALC 208 73.693 140.514 20.533 1.00156.59 6
ATOM 5165 CG1 VALC 208 73.675 141.048 21.954 1.00157.26 6
ATOM 5166 CG2 VALC 208 73.673 138.994 20.528 1.00157.20 6
ATOM 5167 C VALC 208 72.516 142.574 19.767 1.00 69.61 6
ATOM 5168 O VALC 208 72.927 143.204 18.789 1.00 69.37 8
ATOM 5169 N ARGC 209 72.086 143.161 20.879 1.00102.05 7
ATOM 5170 CA ARGC 209 72.062 144.607 21.000 1.00102.46 6
ATOM 5171 CB ARGC 209 73.443 145.164 20.650 1.00 80.09 6
ATOM 5172 CG ARGC 209 74.536 144.483 21.457 1.00 80.04 6
ATOM 5173 CD ARGC 209 75.935 145.015 21.198 1.00 80.98 6
ATOM 5174 NE ARGC 209 76.830 144.580 22.271 1.00 82.65 7
ATOM 5175 CZ ARGC 209 78.128 144.861 22.351 1.00 83.21 6
ATOM 5176 NH1 ARGC 209 78.720 145.58$ 21.408 1.00 83.30 7
ATOM 5177 NH2 ARGC 209 78.831 144.424 23.391 1.00 82.93 7
ATOM 5178 C ARGC 209 70.998 145.061 20.014 1.00103.27 6
ATOM 5179 O ARGC 209 70.996 146.191 19.535 1.00103.27 8
ATOM 5180 N GLUC 210 70.089 144.136 19.727 2.00118.85 7
ATOM 5181 CA GLUC 210 68.984 144.358 18.810 1.00120.27 6
ATOM 5282 CB GLUC 210 69.503 144.441 17.372 1.00280.96 6
ATOM 5183 CG GLUC 2l0 68.659 145.317 16.459 1.00181.78 6
ATOM 5184 CD GLUC 210 68.723 146.784 26.840 2.00182.17 6
ATOM 5185 OE1 GLUC 210 69.801 147.394 16.677 1.00182.81 8
ATOM 5186 OE2 GLUC 210 67.699 147.325 17.308 1.00182.12 8
ATOM 5187 C GLUC 210 68.034 143.163 18.970 1.00120.32 6
ATOM 5188 O GLUC 210 66.988 143.079 28.319 1.00120.10 8
ATOM 5189 N LEUC 211 68.427 142.237 19.843 1.00116.71 7
ATOM 5190 CA LEUC 212 67.643 141.041 20.155 1.00125.98 6
ATOM 5191 CB LEUC 211 68.133 139.830 19.357 1.00135.44 6
ATOM 5192 CG LEUC 211 67.869 138.488 20.064 1.00236.01 6
ATOM 5193 CD1 LEUC 211 66.374 138.278 20.222 1.00137.05 6
ATOM 5194 CD2 LEUC 211 68.488 137.344 19.284 1.00136.63 6
ATOM 5195 C LEUC 211 67.794 140.719 21.633 1.00114.77 6
ATOM 5196 O LEUC 211 66.827 140.715 22.398 1.00114.10 8
ATOM 5197 N SERC 212 69.033 140.437 22.010 1.00109.57 7
ATOM 5198 CA SERC 212 69.373 140.091 23.375 1.00109.17 6
ATOM 5199 CB SERC 212 70.315 138.897 23.368 1.00 83.30 6
93

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5200 OG SER C 212 71.413 139.150 22.509 1.00 83.23
ATOM 5201 C SER C 212 70.036 141.259 24.084 1.00109.05
ATOM 5202 0 SER C 212 71.153 141.650 23.747 1.00109.05
ATOM 5203 N ALA C 213 69.342 141.814 25.070 1.00 89.80
ATOM 5204 CA ALA C 213 69.866 142.935 25.834 1.00 88.67
ATOM 5205 CB ALA C 213 69.444 144.247 25.191 1.00119.25
ATOM 5206 C ALA C 213 69.365 142.870 27.268 1.00 88.05
ATOM 5207 0 ALA C 213 70.162 142.791 28.203 1.00 87.84
ATOM 5208 N TYR C 214 68.046 142.888 27.438 1.00129.04
ATOM 5209 CA TYR C 214 67.468 142.842 28.771 1.00128.86
ATOM 5210 CB TYR C 214 65.933 142.921 28.712 1.00 81.89
ATOM 5211 CG TYR C 214 65.215 141.897 27.840 1.00 80.30
ATOM 5212 CD1 TYR C 214 65.777 140.642 27.553 1.00 79.07
ATOM 5213 CE1 TYR C 214 65.081 139.705 26.781 1.00 77.16
ATOM 5214 CD2 TYR C 214 63.939 142.175 27.332 1.00 79.27
ATOM 5215 CE2 TYR C 214 63.242 141.247 26.569 1.00 77.29
ATOM 5216 CZ TYR C 214 63.815 140.022 26.294 1.00 76.39
ATOM 5217 OH TYR C 214 63.124 139.140 25.501 1.00 75.89
ATOM 5218 C TYR C 214 67.901 141.630 29.592 1.00128.94
ATOM 5219 0 TYR C 214 67.635 141.554 30.793 1.00129.75
ATOM 5220 N GLY C 215 68.573 140.686 28.948 1.00 75.08
ATOM 5221 CA GLY C 215 69.038 139.515 29.660 1.00 75.27
ATOM 5222 C GLY C 215 70.350 139.026 29.079 1.00 75.58
ATOM 5223 0 GLY C 215 70.350 138.096 28.268 1.00 76.28
ATOM 5224 N ASP C 216 71.458 139.648 29.489 1.00 85.58
ATOM 5225 CA ASP C 216 72.804 139.300 29.007 1.00 85.27
ATOM 5226 CB ASP C 216 73.791 139.261 30.174 1.00131.92
ATOM 5227 CG ASP C 216 73.854 140.569 30.922 1.00132.48
ATOM 5228 OD1 ASP C 216 72.816 140.969 31.485 1.00132.94
ATOM 5229 OD2 ASP C 216 74.937 141.197 30.945 1.00132.30
ATOM 5230 C ASP C 216 72.876 137.972 28.266 1.00 84.97
ATOM 5231 0 ASP C 226 72.381 137.829 27.146 1.00 84.91
ATOM 5232 N LEU C 217 73.514 136.998 28.894 1.00 77.58
ATOM 5233 CA LEU C 217 73.623 135.687 28.289 1.00 77.89
ATOM 5234 CB LEU C 217 72.237 135.044 28.190 1.00 80.27
ATOM 5235 CG LEU C 217 71.155 135.447 29.201 1.00 79.36
ATOM 5236 CD1 LEU C 217 69.855 134.737 28.857 1.00 78.34
ATOM 5237 CD2 LEU C 217 71.590 135.106 30.617 1.00 78.99
ATOM 5238 C LEU C 217 74.189 135.836 26.887 1.00 78.60
ATOM 5239 0 LEU C 217 74.174 134.882 26.111 1.00 79.02
ATOM 5240 N VAL C 218 74.698 137.022 26.562 1.00130.'42
ATOM 5241 CA VAL C 218 75.226 137.263 25.225 1.00131.09
ATOM 5242 CB VAL C 228 74.759 138.612 24.678 1.00 90.38
ATOM 5243 CG1 VAL C 218 75.311 138.810 23.279 1.00 90.04
ATOM 5244 CG2 VAL C 218 73.247 138.664 24.660 1.00 89.65
ATOM 5245 C VAL C 218 76.730 137.185 25.009 1.00131.97
ATOM 5246 0 VAL C 218 77.164 136.633 24.000 1.00132.21
ATOM 5247 N GLN C 219 77.527 137.744 25.916 1.00152.90
ATOM 5248 CA GLN C 219 78.980 137.695 25.747 1.00153.25
ATOM 5249 CB GLN C 219 79.700 137.907 27.080 1.00136.00
ATOM 5250 CG GLN C 219 79.550 139.285 27.687 1.00135.38
ATOM 5251 CD GLN C 219 80.500 139.495 28.856 1.00136.08
ATOM 5252 OE1 GLN C 219 81.719 139.563 28.675 1.00136.00
ATOM 5253 NE2 GLN C 219 79.948 139.586 30.063 1.00136.33
ATOM 5254 C GLN C 219 79.402 136.345 25.168 1.00153.29
ATOM 5255 0 GLN C 219 80.380 136.250 24.424 1.00153.73
94

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5256 N GLY C 220 78.649 135.305 25.516 1.00 75.93
7
ATOM 5257 CA GLY C 220 78.941 133.974 25.027 1.00 77.21
6
ATOM 5258 C GLY C 220 78.353 133.704 23.653 1.00 78.18
6
ATOM 5259 0 GLY C 220 78.902 132.907 22.886 1.00 78.07
8
ATOM 5260 N LEU C 221 77.229 134.342 23.338 1.00 83.09
7
ATOM 5261 CA LEU C 221 76.611 134.157 22.02 1.00 84.37
9 6
ATOM 5262 CB LEU C 221 75.195 134.734 22.011 1.00 57.92
6
ATOM; 5263 CG LEU C 221 74.439 134.511 20.695 1.00 57.02
6
ATOM 5264 CD1 LEU C 221 73.967 133.054 20.628 1.00 56.56
6
ATOM 5265 CD2 LEU C 221 73.259 135.474 20.585 1.00 56.14
6
ATOM 5266 C LEU C 221 77.476 134.892 21.003 1.00 85.81
6
ATOM 5267 0 LEU C 221 77.161 134.943 19.812 2.00 86.07
8
ATOM 5268 N LEU C 222 78.578 135.460 21.479 1.00 56.83
7
ATOM 5269 CA LEU C 222 79.482 136.293 20.615 1.00 58.88
6
ATOM 5270 CB LEU C 222 79.536 137.662 21.056 1.00 71.27
6
ATOM 5271 CG LEU C 222 78.229 138.468 20.951 1.00 70.06
6
ATOM 5272 CD1 LEU C 222 78.407 139.815 21.614 2.00 69.38
6
ATOM 5273 CD2 LEU C 222 77.833 138.649 19.497 1.00 68.39
6
ATOM 5274 C LEU C 222 80.888 135.593 20.583 2.00 61.05
6
ATOM 5275 0 LEU C 222 81.616 135.797 19.620 1.00 62.44
8
ATOM 5276 N ASP C 223 81.270 134.848 21.617 1.00249.38
7
ATOM 5277 CA ASP C 223 82.604 134.236 21.662 1.00151.90
6
ATOM 5278 CB ASP C 223 82.637 133.082 22.671 1.00208.87
6
ATOM 5279 CG ASP C 223 82.824 133.561 24.099 1.00208.87
6
ATOM 5280 OD1 ASP C 223 83.824 134.261 24.364 1.00208.87
8
ATOM 5281 OD2 ASP C 223 81.975 133.236 24.956 1.00208.87
8
ATOM 5282 C ASP C 223 83.092 133.731 20.303 1.00152.75
6
ATOM 5283 0 ASP C 223 84.259 133.916 19.950 1.00152.57
8
ATOM 5284 N GLU C 224 82.202 133.089 19.549 1.00 84.62
7
ATOM 5285 CA GLU C 224 82.549 132.576 18.228 1.00 86.27
6
ATOM 5286 CB GLU C 224 81.940 131.186 18.019 1.00208.87
6
ATOM 5287 CG GLU C 224 82.704 130.066 18.706 1.00208.87
6
ATOM 5288 CD GLU C 224 84.113 129.907 18.162 1.00208.87
6
ATOM 5289 OE1 GLU C 224 84.265 129.623 16.955 1.00208.87
8
ATOM 5290 OE2 GLU C 224 85.073 130.069 18.943 1.00208.87
8
ATOM 5291 C GLU C 224 82.085 133.520 17.117 1.00 86.52
6
ATOM 5292 0 GLU C 224 81.729 133.075 16.020 1.00 85.93
8
ATOM 5293 N ALA C 225 82.091 234.822 17.406 1.00175.86
7
ATOM 5294 CA ALA C 225 81.689 135.831 16.426 1.00176,34
6
ATOM 5295 CB ALA C 225 81.941 237.230 16.961 1.00 78.67
6
ATOM 5296 C ALA C 225 82.538 135.586 15.199 1.00176.29
6
ATOM 5297 O ALA C 225 82.205 136.013 14.095 1.00176.67
8
ATOM 5298 N VAL C 226 83.657 134.904 15.419 1.00 98.39
7
ATOM 5299 CA VAL C 226 84.548 134.549 14.341 1.00 97.87
6
ATOM 5300 CB VAL C 226 85.628 133.561 14.831 1.00 88.32
6
ATOM 5301 CG1 VAL C 226 86.463 134.211 15.916 1.00 88.30
6
ATOM 5302 CG2 VAL C 226 84.985 132.302 15.377 1.00 88.42
6
ATOM 5303 C VAL C 226 83.635 133.881 13.319 1.00 97.91
6
ATOM 5304 O VAL C 226 83.751 134.119 12.119 1.00 98.63
8
ATOM 5305 N LEU C 227 82.709 133.060 13.814 1.00138.81
7
ATOM 5306 CA LEU C 227 81.743 132.375 12.964 1.00139.05
6
ATOM 5307 CB LEU C 227 80.978 131.303 13.755 1.00116.19
6
ATOM 5308 CG LEU C 227 81.749 130.059 14.213 1.00117.20
6
ATOM 5309 CD1 LEU C 227 80.814 129.071 14.911 1.00116.00
6
ATOM 5310 CD2 LEU C 227 82.392 129.404 13.005 2.00117.61
6
ATOM 5311 C LEU C 227 80.763 133.415 12.437 1.00138.64
6

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5312 0 LEU C 227 80.368 133.379 11.275 1.00138.91 8
ATOM 5313 N ALA C 228 80.384 134.346 13.306 1.00 65.37 7
ATOM 5314 CA ALA C 228 79.458 135.424 12.952 1.00 65.33 6
ATOM 5315 . ALA C 228 78.777 135.959 14.211 1.00126.31 6
CB
ATOM 5316 C ALA C 228 80.143 136.578 12.213 1.00 65.44 6
ATOM 5317 0 ALA C 228 79.798 137.742 12.416 1.00 65.01 8
ATOM 5318 N MET C 229 81.103 136.245 11.353 1.00105.03 7
ATOM 5319 CA MET C 229 81.842 137.245 10.588 1.00104.42 6
ATOM 5320 CB MET C 229 83.326 137.179 10.950 1.00208.87 6
ATOM 5321 CG MET C 229 83.622 137.635 12.361 1.00208.87 6
ATOM 5322 SD MET C 229 82.935 139.271 12.666 1.00208.87 16
ATOM 5323 CE MET C 229 84.185 140.325 11.847 1.00208.87 6
ATOM 5324 C MET C 229 81.686 137.148 9.068 1.00102.40 6
ATOM 5325 O MET C 229 82.146 138.029 8.341 1.00101.26 8
ATOM 5326 N ARG C 230 81.046 136.085 8.590 1.00 66.08 7
ATOM 5327 CA ARG C 230 80.836 135.900 7.157 1.00 65.23 6
ATOM 5328 CB ARG C 230 82.188 135.731 6.454 1.00105.16 6
ATOM 5329 CG ARG C 230 83.196 134.900 7.239 1.00106.81 6
ATOM 5330 CD ARG C 230 84.492 134.702 6.457 1.00107.45 6
ATOM 5331 NE ARG C 230 85.550 134.118 7.281. 1.00108.11 7
ATOM 5332 CZ ARG C 230 86.127 134.732 8.316 1.00108.21 6
ATOM 5333 NH1 ARG C 230 85.732 135.953 8.659 1.00107.49 7
ATOM 5334 NH2 ARG C 230 87.069 134.123 9.010 1.00109.17 7
ATOM 5335 C ARG C 230 79.937 134.708 6.824 1.00 63.26 6
ATOM 5336 0 ARG C 230 80.314 133.558 7.054 1.00 63.36 8
ATOM 5337 N PRO C 231 78.733 134.962 6.275 1.00140.24 7
ATOM 5338 CD PRO C 231 78.185 136.252 5.826 1.00 42.06 6
ATOM 5339 CA PRO C 231 77.822 133.864 5.926 1.00138.58 6
ATOM 5340 CB PRO C 231 76.584 134.590 5.399 1.00 40.00 6
ATOM 5341 CG PRO C 231 77.254 135.818 4.804 1.00 41.60 6
ATOM 5342 C PRO C 231 78.474 132.999 4.866 1.00137.86 6
ATOM 5343 0 PRO C 231 79.656 133.162 4.589 1.00139.31 8
ATOM 5344 N GLU C 232 77.715 132.091 4.267 1.00 77.90 7
ATOM 5345 CA GLU C 232 78.268 131.224 3.231 1.00 75.34 6
ATOM 5346 CB GLU C 232 78.744 132.063 2.036 1.00 99.87 6
ATOM 5347 CG GLU C 232 79.165 131.268 0.789 1.00100.05 6
ATOM 5348 CD GLU C 232 80.578 130.706 0.863 1.00 99.76 6
ATOM 5349 OE1 GLU C 232 80.817 229.769 1.652 1.00 99.37 8
ATOM 5350 OE2 GLU C 232 81.451 131.208 0.122 1.00100.08 8
ATOM 5351 C GLU C 232 79.435 130.438 3.810 1.00 73.40 6
ATOM 5352 0 GLU C 232 79.314 129.245 4.083 1.00 73.09 8
ATOM 5353 N GLU C 233 80.569 131.110 3.985 1.00 73.71 7
ATOM 5354 CA GLU C 233 81.753 130.488 4.561 1.00 71.34 6
ATOM 5355 CB GLU C 233 82.771 131.564 4.953 1.00 69.36 6
ATOM 5356 CG GLU C 233 84.231 131.127 4.910 1.00 67.28 6
ATOM 5357 CD GLU C 233 84.578 130.039 5.918 1.00 66.29 6
ATOM 5358 OE1 GLU C 233 84.483 130.293 7.137 1.00 65.59 8
ATOM 5359 OE2 GLU C 233 84.957 128.927 5.493 1.00 66.13 8
ATOM 5360 C GLU C 233 81.224 129.791 5.803 1.00 70.23 6
ATOM 5361 O GLU C 233 81.790 128.802 6.274 1.00 70.93 8
ATOM 5362 N ALA C 234 80.107 130.323 6.302 1.00 99.90 7
ATOM 5363 CA ALA C 234 79.418 129.814 7.483 1.00 97.62 6
.
ATOM 5364 CB ALA C 234 78.408 130.846 7.971 1.00 76.06 6
ATOM 5365 C ALA C 23~ 78.719 128.473 7.229 1.00 95.90 6
ATOM 5366 O ALA C 234 79.022 127.481 7.899 1.00 95.75 8
ATOM 5367 N MET C 235 77.783 128.442 6.278 1.00 65.06 7
96

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5368 CA METC 235 77.074 127.203 5.954 1.00 62.59
6
ATOM 5369 CB METC 235 76.397 127.298 4.594 1.00 48.98
6
ATOM 5370 CG METC 235 75.708 128.597 4.319 1.00 48.48
6
ATOM 5371 SD METC 235 74.890 128.508 2.715 1.00 48.43
16
ATOM 5372 CE METC 235 73.125 128.459 3.250 1.00 47.57
6
ATOM 5373 C METC 235 78.097 126.075 5.879 1.00 62.17
6
ATOM 5374 0 METC 235 77.866 124.969 6.374 1.00 61.98
8
ATOM 5375 N VALC 236 79.230 126.374 5.247 1.00 38.65
7
ATOM 5376 CA VALC 236 80.299 125.405 5.082 1.00 36.92
6
ATOM 5377 CB VALC 236 81.394 125.946 4.140 1.00 13.87
6
ATOM 5378 CG1 VALC 236 82.629 125.036 4.205 1.00 13.87
6
ATOM 5379 CG2 VALC 236 80.836 126.039 2.695 1.00 13.87
6
ATOM 5380 C VALC 236 80.909 124.982 6.409 1.00 37.57
6
ATOM 5381 0 VALC 236 81.212 123.811 6.596 1.00 37.03
8
ATOM 5382 N ARGC 237 81.097 125.917 7.331 1.00101.36
7
ATOM 5383 CA ARGC 237 81.646 125.545 8.625 1.00103.24
6
ATOM 5384 CB ARGC 237 81.927 126.786 9.464 1.00117.69
6
ATOM 5385 CG ARGC 237 82.957 127.707 8.842 1.00119.98
6
ATOM 5386 CD ARGC 237 84.320 127.050 8.754 1.00121.93
6
ATOM 5387 NE ARGC 237 85.291 127.934 8.115 1.00125.29
7
ATOM 5388 CZ ARGC 237 86.586 127.662 7.993 1.00126.39
6
ATOM 5389 NH1 ARGC 237 87.075 126.526 8.471 1.00127.88
7
ATOM 5390 NH2 ARGC 237 87.393 128.525 7.390 1.00126.26
7
ATOM 5391 C ARGC 237 80.608 124.657 9.305 1.00103.63
6
ATOM 5392 0 ARGC 237 80.951 123.662 9.943 1.00104.18
8
ATOM 5393 N LEUC 238 79.337 125.022 9.157 1.00 56.15
7
ATOM 5394 CA LEUC 238 78.240 124.239 9.723 1.00 56.26
6
ATOM 5395 CB LEUC 238 76.903 124.969 9.511 1.00 20.59
6
ATOM 5396 CG LEUC 238 75.548 124.426 9.996 1.00 18.61
6
ATOM 5397 CD1 LEUC 238 75.416 124.515 11.505 1.00 17.53
6
ATOM 5398 CD2 LEUC 238 74.454 125.252 9.356 1.00 17.41
6
ATOM 5399 C LEUC 238 78.239 122.899 8.977 1.00 57.78
6
ATOM 5400 O LEUC 238 78.290 121.838 9.594 1.00 57.62
8
ATOM 5401 N PHEC 239 78.194 122.951 7.648 1.00 50.91
7
ATOM 5402 CA PHEC 239 78.203 121.734 6.844 1.00 52.69
6
ATOM 5403 CB PHEC 239 78.419 122.071 5.367 1.00 84.65
6
ATOM 5404 CG PHEC 239 77.904 121.022 4.414 1.00 84.64
6
ATOM 5405 CD1 PHEC 239 76.800 121.287 3.594 1.00 85.15
6
ATOM 5406 CD2 PHEC 239 78.509 119.773 4.333 1.00 83.76
6
ATOM 5407 CE1 PHEC 239 76.307 120.323 2.707 1.00 85.25
6
ATOM 5408 CE2 PHEC 239 78.025 118.799 3.450 1.00 83.75
6
ATOM 5409 CZ PHEC 239 76.920 119.076 2.636 1.00 84.76
6
ATOM 5410 C PHEC 239 79.358 120.871 7.342 1.00 54.23
6
ATOM 5411 0 PHEC 239 79.191 119.690 7.643 1.00 53.69
8
ATOM 5412 N THRC 240 80.531 121.486 7.441 1.00 49.83
7
ATOM 5413 CA THRC 240 81.729 120.801 7.891 1.00 53.38
6
ATOM 5414 CB THRC 240 82.966 121.696 7.735 1.00 75.67
6
ATOM 5415 OG1 THRC 240 83.025 122.197 6.392 1.00 76.24
8
ATOM 5416 CG2 THRC 240 84.234 120.906 8.016 1.00 75.21
6
ATOM 5417 C THRC 240 81.583 120.389 9.344 1.00 56.09
6
ATOM 5418 O THRC 240 82.509 119.860 9.945 1.00 56.86
8
ATOM 5419 N LEUC 241 80.412 120.642 9.912 1.00 88.04
7
ATOM 5420 CA LEUC 241 80.148 120.260 11.290 1.00 92.43
6
ATOM 5421 CB LEUC 241 79.587 121.432 12.087 1.00 69.60
6
ATOM 5422 CG LEUC 241 80.587 122.299 12.847 1.00 68.57
6
ATOM 5423 CD1 LEUC 241 79.844 123.466 13.468 1.00 68.16
6
97

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5424 CD2 LEUC 241 81.283 121.469 13.917 1.00 68.18
ATOM 5425 C LEUC 241 79.156 119.113 11.286 1.00 96.01
ATOM 5426 0 LEUC 241 78.653 118.698 12.333 1.00 96.09
ATOM 5427 N LEUC 242 78.873 118.623 10.083 1.00 88.50
ATOM 5428 CA LEUC 242 77.969 117.499 9.881 1.00 92.67
ATOM 5429 CB LEUC 242 76.785 117.901 8.990 1.00161.63
ATOM 5430 CG LEUC 242 75.503 118.336 9.717 1.00162.36
ATOM 5431 CD1 LEUC 242 75.769 119.543 10.604 1.00162.25
ATOM 5432 CD2 LEUC 242 74.425 118.650 8.692 1.00162.82
ATOM 5433 C LEUC 242 78.739 116.336 9.255 1.00 95.23
ATOM 5434 0 LEUC 242 79.088 116.353 8.070 1.00 96.06
ATOM 5435 N ARGC 243 79.011 115.340 10.090 1.00208.87
ATOM 5436 CA ARGC 243 79.737 114.140 9.700 1.00208.87
ATOM 5437 CB ARGC 243 79.738 113.152 10.874 1.00203.59
ATOM 5438 CG ARGC 243 80.592 111.913 10.686 1.00206.59
ATOM 5439 CD ARGC 243 80.534 111.041 11.922 1.00208.78
ATOM 5440 NE ARGC 243 81.468 109.924 11.856 1.00208.87
ATOM 5441 CZ ARGC 243 81.642 109.043 12.836 1.00208.87
ATOM 5442 NH1 ARGC 243 80.942 109.152 13.958 1.00208.87
ATOM 5443 NH2 ARGC 243 82.519 108.057 12.699 1.00208.87
ATOM 5444 C ARGC 243 79.086 113.497 8.477 1.00208.87
ATOM 5445 0 ARGC 243 77.873 113.592 8.289 1.00208.87
ATOM 5446 N PROC 244 79.890 112.842 7.621 1.00168.13
ATOM 5447 CD PROC 244 79.366 111.947 6.570 1.00208.87
ATOM 5448 CA PROC 244 81.341 112.685 7.754 1.00167.55
ATOM 5449 CB PROC 244 81.594 111.349 7.073 1.00208.87
ATOM 5450 CG PROC 244 80.636 111.410 5.925 1.00208.87
ATOM 5451 C PROC 244 82.105 113.829 7.092 1.00167.24
ATOM 5452 0 PROC 244 81.603 114.949 6.984 1.00167.12
ATOM 5453 N GLYC 245 83.321 113.533 6.649 1.00141.10
ATOM 5454 CA GLYC 245 84.130 114.541 5.997 1.00140.05
ATOM 5455 C GLYC 245 83.762 114.705 4.536 1.00138.99
ATOM 5456 O GLYC 245 84.517 114.311 3.649 1.00139.36
ATOM 5457 N ASPC 246 82.587 115.270 4.282 1.00 91.31
ATOM 5458 CA ASPC 246 82.136 115.501 2.915 1.00 90.57
ATOM 5459 CB ASPC 246 80.625 115.749 2.900 1.00180.76
ATOM 5460 CG ASPC 246 79.827 114.481 3.112 1.00181.48
ATOM 5461 OD1 ASPC 246 80.029 113.518 2.343 1.00181.22
ATOM 5462 OD2 ASPC 246 78.993 114.449 4.041 1.00181.84
ATOM 5463 C ASPC 246 82.883 116.710 2.339 1.00 89.74
ATOM 5464 0 ASPC 246 83.858 117.180 2.927 1.00 90.14
ATOM 5465 N PROC 247 82.446 117.227 1.182 1.00114.04
ATOM 5466 CD PROC 247 81.519 116.670 0.181 1.00194.03
ATOM 5467 CA PROC 247 83.163 118.382 0.634 1.00113.17
ATOM 5468 CB PROC 247 82.840 118.302 -0.852 1.00194.32
ATOM 5469 CG PROC 247 81.437 117.785 -0.839 1.00194.34
ATOM 5470 C PROC 247 82.747 119.722 1.238 1.00111.52
ATOM 5471 0 PROC 247 81.607 120.157 1.066 1.00111.58
ATOM 5472 N PROC 248 83.668 120.397 1.955 1.00 92.49
ATOM 5473 CD PROC 248 84.997 119.930 2.389 1.00 76.77
ATOM 5474 CA PROC 248 83.357 121.692 2.566 1.00 90.62
ATOM 5475 CB PROC 248 84.686 122.105 3.185 1.00 75.12
ATOM 5476 CG PROC 248 85.268 120.801 3.596 1.00 75.76
ATOM 5477 C PROC 248 82.867 122.693 1.530 1.00 88.92
ATOM 5478 0 PROC 248 82.450 123.794 1.862 1.00 89.53
ATOM 5479 N LYSC 249 82.917 122.301 0.267 1.00 61.79
9~

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5480 CA LYS C 249 82.472 123.171 -0.805 1.00 59.51
6
ATOM 5481 CB LYS C 249 82.371 122.373 -2.107 1.00157.18
6
ATOM 5482 CG LYS C 249 82.144 223.214 -3.355 1.00158.15
6
ATOM 5483 CD LYS C 249 82.300 122.371 -4.617 1.00157.76
6
ATOM 5484 CE LYS C 249 82.232 123.225 -5.873 1.00157.27
6
ATOM 5485 NZ LYS C 249 82.505 122.427 -7.101 1.00156.24
7
ATOM 5486 C LYS C 249 81.124 123.798 -0.468 1.00 58.08
6
ATOM 5487 O LYS C 249 80.211 123.208 -0.023 1.00 57.68
8
ATOM 5488 N LYS C 250 81.028 125.112 -0.661 1.00 52.07
7
ATOM 5489 CA LYS C 250 79.811 125.902 -0.424 1.00 51.13
6
ATOM 5490 CB LYS C 250 80.003 127.283 -1.044 1.00 84.19
6
ATOM 5491 CG LYS C 250 80.498 127.165 -2.489 1.00 84.65
6
ATOM 5492 CD LYS C 250 80.484 128.462 -3.273 1.00 84.80
6
ATOM 5493 CE LYS C 250 80.930 128.190 -4.711 1.00 84.35
6
ATOM 5494 NZ LYS C 250 80.889 129.393 -5.590 1.00 85.51
7
ATOM 5495 C LYS C 250 78.621 125.230 -1.113 1.00 49.81
6
ATOM 5496 0 LYS C 250 78.672 124.996 -2.319 1.00 49.67
8
ATOM 5497 N ASP C 252 77.544 124.940 -0.384 1.00208.87
7
ATOM 5498 CA ASP C 251 76.402 124.280 -1.018 1.00208.87
~ 6
ATOM 5499 CB ASP C 251 76.330 122.816 -0.574 1.00 84.58
6
ATOM 5500 CG ASP C 251 76.918 121.878 -1.606 1.00 84.74
6
ATOM 5501 OD1 ASP C 251 76.399 121.850 -2.741 1.00 83.90
8
ATOM 5502 OD2 ASP C 251 77.902 221.177 -1.293 1.00 85.22
8
ATOM 5503 C ASP C 251 75.005 124.887 -0.930 1.00208.87
6
ATOM 5504 O ASP C 251 74.339 125.020 -1.956 1.00208.87
8
ATOM 5505 N LYS C 252 74.553 125.244 0.271 1.00 89.65
7
ATOM 5506 CA LYS C 252 73.211 125.805 0.443 1.00 86.82
6
ATOM 5507 CB LYS C 252 72.848 126.755 -0.715 1.00 69.45
6
ATOM 5508 CG LYS C 252 71.372 127.168 -0.758 1.00 69.86
6
ATOM 5509 CD LYS C 252 71.070 128.283 -1.777 1.00 69.45
6
ATOM 5510 CE LYS C 252 69.607 128.763 -1.668 1.00 69.19
~ 6
ATOM 5511 NZ LYS C 252 69.233 129.903 -2.562 1.00 67.70
7
ATOM 5512 C LYS C 252 72.217 124.648 0.495 1.00 85.53
6
ATOM 5513 O LYS C 252 71.167 124.741 1..127 1.00 85.56
8
ATOM 5514 N ALA C 253 72.562 123.557 -0.179 1.00104.71
7
ATOM 5515 CA ALA C 253 71.717 122.373 -0.200 1.00102.89
6
ATOM 5516 CB ALA C 253 72.163 121.442 -1.294 1.00 66.25
6
ATOM 5517 C ALA C 253 71.848 121.695 1.156 1.00101.99
6
ATOM 5518 O ALA C 253 72.812 121.965 1.881 1.00103.16
8
ATOM 5519 N LEU C 254 70.898 120.814 1.486 1.00 67.47
7
ATOM 5520 CA LEU C 254 70.883 120.112 2.779 1.00 64.85
6
ATOM 5521 CB LEU C 254 72.302 119.723 3.209 1.00 30.25
6
ATOM 5522 CG LEU C 254 72.776 119.888 4.649 1.00 28.08
6
ATOM 5523 CD1 LEU C 254 71.826 119.234 5.637 1.00 27.43
6
ATOM 5524 CD2 LEU C 254 74.148 119.271 4.739 1.00 27.06
6
ATOM 5525 C LEU C 254 70.292 121.095 3.760 1.00 64.57
6
ATOM 5526 O LEU C 254 69.409 120.749 4.542 1.00 64.88
8
ATOM 5527 N ALA C 255 70.823 122.318 3.710 1.00 46.43
7
ATOM 5528 CA ALA C 255 70.357 123.440 4.510 1.00 45.16
6
ATOM 5529 CB ALA C 255 71.494 124.433 4.786 1.00 13.87
6
ATOM 5530 C ALA C 255 69.338 124.024 3.539 1.00 45.33
6
ATOM 5531 0 ALA C 255 68.816 125.113 3.713 1.00 46.26
8
ATOM 5532 N TYR C 256 69.089 123.253 2.-490 1.00 49.19
7
ATOM 5533 CA TYR C 256 68.123 123.574 1.455 1.00 49.69
6
ATOM 5534 CB TYR C 256 68.525 122.884 0.152 1.00113.70
6
ATOM 5535 CG TYR C 256 68.211 123.616 -1.137 1.00116.52
6
99

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5536 CD1 TYR C 256 69.006 124.680 -1.570 1.00117.43
6
ATOM 5537 CE1 TYR C 256 68.790 125.288 -2.814 1.00117.60
6
ATOM 5538 CD2 TYR C 256 67.179 123.183 -1.974 1.00117.49
6
ATOM 5539 CE2 TYR C 256 66.952 123.783 -3.219 2.00117.99
6
ATOM 5540 CZ TYR C 256 67.764 124.832 -3.635 1.00117.79
6
ATOM 5541 OH TYR C 256 67.572 125.402 -4.878 1.00116.97
8
ATOM 5542 C TYR C 256 66.885 122.887 2.013 1.00 49.09
6
ATOM 5543 0 TYR C 256 65.848 122.828 1.369 1.00 48.84
8
ATOM 5544 N LEU C 257 67.043 122.339 3.216 1.00 77.53
7
ATOM 5545 CA LEU C 257 66.002 121.617 3.947 1.00 77.28
6
ATOM 5546 CB LEU C 257 66.437 120.174 4.204 1.00116.17
6
ATOM 5547 CG LEU C 257 66.891 119.307 3.028 1.00117.61
6
ATOM 5548 CD1 LEU C 257 67.935 118.302 3.512 1.00118.96
6
ATOM 5549 CD2 LEU C 257 65.683 118.612 2.400 1.00116.94
6
ATOM 5550 C LEU C 257 65.889 122.323 5.281 1.00 76.11
6
ATOM 5551 0 LEU C 257 65.799 123.544 5.314 1.00 76.74
8
ATOM 5552 N PHE C 258 65.934 121.543 6.366 1.00 66.70
7
ATOM 5553 CA PHE C 258 65.856 122.057 7.739 1.00 65.52
6
ATOM 5554 CB PHE C 258 67.227 122.053 8.407 1.00 60.51
6
ATOM 5555 CG PHE C 258 67.903 120.721 8.405 1.00 61.13
6
ATOM 5556 CD1 PHE C 258 68.146 120.052 7.216 1.00 60.44
6
ATOM 5557 CD2 PHE C 258 68.352 120.159 9.594 1.00 61.98
6
ATOM 5558 CE1 PHE C 258 68.829 118.853 7.223 1.00 60.93
6
ATOM 5559 CE2 PHE C 258 69.041 118.954 9.601 1.00 61.63
6
ATOM 5560 CZ PHE C 258 69.280 118.302 8.413 1.00 61.42
6
ATOM 5561 C PHE C 258 65.349 123.479 7.735 1.00 65.21
6
ATOM 5562 0 PHE C 258 65.923 124.359 8.385 1.00 65.27
8
ATOM 5563 N GLY C 259 64.269 123.685 6.990 1.00 42.12
7
ATOM 5564 CA GLY C 259 63.679 124.995 6.850 1.00 40.93
6
ATOM 5565 C GLY C 259 63.238 125.119 5.408 1.00 40.90
6
ATOM 5566 0 GLY C 259 62.037 125.170 5.129 1.00 40.65
8
ATOM 5567 N LEU C 260 64.200 125.147 4.485 1.00 31.09
7
ATOM 5568 CA LEU C 260 63.864 125.273 3.067 1.00 32.91
6
ATOM 5569 CB LEU C 260 65.137 125.288 2.195 1.00 54.75
6
ATOM 5570 CG LEU C 260 65.062 125.650 0.688 1.00 53.93
6
ATOM 5571 CD1 LEU C 260 66.449 125.979 0.197 1.00 54.88
6
ATOM 5572 CD2 LEU C 260 64.503 124.532 -0.167 1.00 53.12
6
ATOM 5573 C LEU C 260 62.942 124.139 2.627 1.00 33.56
6
ATOM 5574 0 LEU C 260 62.855 123.107 3.286 1.00 32.20
8
ATOM 5575 N LEU C 261 62.252 124.360 1.514 1.00 74.26
7
ATOM 5576 CA LEU C 261 61.329 123.396 0.923 1.00 76.02
6
ATOM 5577 CB LEU C 261 62.083 122.408 0.015 1.00 80.87
6
ATOM 5578 CG LEU C 261 62.573 121.041 0.505 1.00 80.48
6
ATOM 5579 CD1 LEU C 261 63.112 120.264 -0.688 1.00 80.79
6
ATOM 5580 CD2 LEU C 261 63.641 121.187 1.559 1.00 80.51
6
ATOM 5581 C LEU C 261 60.452 122.621 1.894 1.00 77.26
6
ATOM 5582 0 LEU C 261 60.928 121.854 2.729 1.00 76.99
8
ATOM 5583 N ALA C 262 59.150 122.829 1.751 1.00 44.61
7
ATOM 5584 CA ALA C 262 58.168 122.171 2.580 1.00 45.54
6
ATOM 5585 CB ALA C 262 56.803 122.666 2.190 1.00 13.87
6
ATOM 5586 C ALA C 262 58.260 120.654 2.404 1.00 47.51
6
ATOM 5587 O ALA C 262 57.279 119.934 2.626 1.00 48.30
8
ATOM 5588 N ASP C 263 59.439 120.171 2.009 1.00 48.36
7
ATOM 5589 CA ASP C 263 59.652 118.743 1.785 1.00 51.39
6
ATOM 5590 CB ASP C 263 60.790 118.525 0.783 1.00112.70
6
ATOM 5591 CG ASP C 263 61.786 117.462 1.243 1.00114.08
6
100

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 5592 OD1 ASP C 263 61.369 116.302 1.432 1.00115.21
ATOM 5593 OD2 ASP C 263 62.982 117.786 1.422 1.00113.92
ATOM 5594 C ASP C 263 59.941 117.941 3.043 1.00 52.97
ATOM 5595 0 ASP C 263 59.361 116.875 3.240 1.00 52.61
ATOM 5596 N PRO C 264 60.864 118.421 3.893 1.00 61.10
ATOM 5597 CD PRO C 264 61.992 119.306 3.555 1.00121.93
ATOM 5598 CA PRO C 264 61.175 117.681 5.114 1.00 63.87
ATOM 5599 CB PRO C 264 62.656 117.405 4.942 1.00123.85
ATOM 5600 CG PRO C 264 63.142 118.742 4.415 1.00123.04
ATOM 5601 C PRO C 264 60.885 118.496 6.377 1.00 65.94
ATOM 5602 O PRO C 264 61.380 118.184 7.465 1.00 65.59
ATOM 5603 N LYS C 265 60.082 119.542 6.232 1.00114.70
ATOM 5604 CA LYS C 265 59.762 120.389 7.370 1.00117.68
ATOM 5605 CB LYS C 265 60.964 121.290 7.703 1.00 69.15
ATOM 5606 CG LYS C 265 60.841 122.110 8.987 1.00 68.89
ATOM 5607 CD LYS C 265 61.275 121.307 10.208 1.00 69.00
ATOM 5608 CE LYS C 265 61.219 122.141 11.486 1.00 69.27
ATOM 5609 NZ LYS C 265 61.710 121.388 12.683 1.00 69.17
ATOM 5610 C LYS C 265 58.551 121.253 7.062 1.00120.01
ATOM 5611 0 LYS C 265 58.690 122.360 6.555 1.00119.67
ATOM 5612 N ARG C 266 57.361 120.744 7.345 1.00 80.91.
ATOM 5613 CA ARG C 266 56.165 121.534 7.118 1.00 84.78
ATOM 5614 CB ARG C 266 55.942 121.783 5.618 1.00126.80
ATOM 5615 CG ARG C 266 54.875 120.918 4.943 1.00129.36
ATOM 5616 CD ARG C 266 54.358 121.611 3.679 1.00131.03
ATOM 5617 NE ARG C 266 53.368 120.833 2.937 1.00132.53
ATOM 5618 CZ ARG C 266 52.662 121.309 1.913 1.00133.01
ATOM 5619 NH1 ARG C 266 52.830 122.562 1.506 1.00132.55
ATOM 5620 NH2 ARG C 266 51.783 120.534 1.295 1.00134.02
ATOM 5621 C ARG C 266 54.921 120.928 7.740 1.00 86.59
ATOM 5622 0 ARG C 266 54.568 119.779 7.482 1.00 86.90
ATOM 5623 N TYR C 267 54.265 121.726 8.574 1.00163.69
ATOM 5624 CA TYR C 267 53.044 121.312 9.244 1.00165.06
ATOM 5625 CB TYR C 267 52.944 121.975 10.629 1.00 86.31
ATOM 5626 CG TYR C 267 54.289 122.359 11.228 1.00 86.21
ATOM 5627 CD1 TYR C 267 54.387 123.415 12.128 1.00 87.05
ATOM 5628 CE1 TYR C 267 55.630 123.863 12.590 1.00 86.89
ATOM 5629 CD2 TYR C 267 55.472 121.743 10.815 1.00 85.28
ATOM 5630 CE2 TYR C 267 56.713 122.182 11.267 1.00 85.79
ATOM 5631 CZ TYR C 267 56.785 123.245 12.151 1.00 86.10
ATOM 5632 OH TYR C 267 58.007 123.714 12.572 1.00 85.99
ATOM 5633 C TYR C 267 51.975 121.834 8.286 1.00165.93
ATOM 5634 0 TYR C 267 50.838 122.11.68.669 1.00166.25
ATOM 5635 N ASP C 268 52.406 121.959 7.030 1.00129.89
ATOM 5636 CA ASP C 268 51.617 122.416 5.888 1.00131.45
ATOM 5637 CB ASP C 268 51.233 121.210 5.003 1.00182.27
ATOM 5638 CG ASP C 268 50.760 119.998 5.806 1.00183.34
ATOM 5639 OD1 ASP C 268 51.580 119.405 6.540 1.00183.74
ATOM 5640 OD2 ASP C 268 49.568 119.633 5.694 1.00184.62
ATOM 5641 C ASP C 268 50.382 123.275 6.142 1.00131.54
ATOM 5642 0 ASP C 268 50.344 124.063 7.089 1.00131.27
ATOM 5643 N ALA C 269 49.399 123.121 5.247 1.00198.05
ATOM 5644 CA ALA C 269 48.109 123.819 5.275 1.00197.81
ATOM 5645 CB ALA C 269 47.081 122.971 6.053 1.00 84.50
ATOM 5646 C ALA C 269 48.179 125.228 5.856 1.00197.19
ATOM 5647 0 ALA C 269 48.035 126.228 5.150 1.00197.51
101

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ATOM 5648 N GLY C 270 48.391 125.284 7.160 2.00123.16
7
ATOM 5649 CA GLY C 270 48.487 126.544 7.854 1.00121.84
6
ATOM 5650 C GLY C 270 48.242 126.229 9.311 1.00121.58
6
ATOM 5651 0 GLY C 270 48.893 126.770 10.200 1.00121.86
8
ATOM 5652 N GLU C 271 47.312 125.314 9.548 1.00 56.86
7
ATOM 5653 CA GLU C 271 46.955 124.928 10.900 1.00 56.02
6
ATOM 5654 CB GLU C 271 45.659 124.117 10.870 1.00103.22
6
ATOM 5655 CG GLU C 271 44.493 124.851 10.224 1.00102.91
6
ATOM 5656 CD GLU C 271 44.210 126.195 10.872 1.00103.24
6
ATOM 5657 OE1 GLU C 271 43.798 126.221 12.052 1.00103.46
8
ATOM 5658 OE2 GLU C 271 44.399 127.226 10.196 1.00103.93
8
ATOM 5659 C GLU C 271 48.040 124.144 11.635 1.00 55.94
6
ATOM 5660 0 GLU C 271 48.949 123.584 11.020 1.00 55.00
8
ATOM 5661 N ALA C 272 47.925 124.145 12.963 1.00136.30
7
ATOM 5662 CA ALA C 272 48.822 123.445 13.883 1.00137.12
6
ATOM 5663 CB ALA C 272 48.819 121.943 13.563 1.00 70.85
6
ATOM 5664 C ALA C 272 50.265 123.957 13.996 1.00138.00
6
ATOM 5665 0 ALA C 272 51.048 123.432 14.792 1.00138.36
8
ATOM 5666 N GLY C 273 50.622 124.980 13.225 1.00107.29
7
ATOM 5667 CA GLY C 273 51.981 125.498 13.293 1.00107.57
6
ATOM 5668 C GLY C 273 52.072 127.006 13.427 1.00107.38
6
ATOM 5669 0 GLY C 273 52.775 127.532 14.290 1.00106.84
8
ATOM 5670 N ARG C 274 51.371 127.708 12.548 1.00163.77
7
ATOM 5671 CA ARG C 274 51.368 129.155 12.597 1.00164.91
6
ATOM 5672 CB ARG C 274 50.629 129.729 11.382 1.00130.45
6
ATOM 5673 CG ARG C 274 49.139 129.418 11.330 1.00130.94
6
ATOM 5674 CD ARG C 274 48.559 129.727 9.960 1.00131.16
6
ATOM 5675 NE ARG C 274 47.121 129.490 9.909 1.00132.12
7
ATOM 5676 CZ ARG C 274 46.406 129.467 8.788 1.00132.41
6
ATOM 5677 NH1 ARG C 274 46.998 129.664 7.614 1.00131.64
7
ATOM 5678 NH2 ARG C 274 45.098 129.252 8.842 1.00133.00
7
ATOM 5679 C ARG C 274 50.648 129.516 13.886 1.00265.08
6
ATOM 5680 0 ARG C 274 51.175 130.255 14.717 1.00165.31
8
ATOM 5681 N TYR C 275 49.450 128.965 14.055 1.00126.35
7
ATOM 5682 CA TYR C 275 48.652 129.214 15.243 1.00127.24
6
ATOM 5683 CB TYR C 275 47.471 128.246 15.304 1.00208.45
6
ATOM 5684 CG TYR C 275 46.313 128.627 14.412 1.00208.87
6
ATOM 5685 CD1 TYR C 275 46.488 128.794 13.040 1.00208.87
6
ATOM 5686 CE1 TYR C 275 45.421 129.137 12.213 1.00208.87
6
ATOM 5687 CD2 TYR C 275 45.036 128.812 14.940 1.00208.87
6
ATOM 5688 CE2 TYR C 275 43.960 129.154 14.122 1.00208.87
6
ATOM 5689 CZ TYR C 275 44.160 129.315 12.759 1.00208.87
6
ATOM 5690 OH TYR C 275 43.099 129.642 11.942 1.00208.87
8
ATOM 5691 C TYR C 275 49.509 129..04416.482 1.00127.57
6
ATOM 5692 0 TYR C 275 49.534 129.906 17.358 1.00128.16
8
ATOM 5693 N ALA C 276 50.225 127.932 16.550 1.00 62.61
7
ATOM 5694 CA ALA C 276 51.067 127.683 17.703 1.00 61.43
6
ATOM 5695 CB ALA C 276 51.898 126.435 17.470 1.00160.70
6
ATOM 5696 C ALA C 276 51.977 128.872 18.019 1.00 60.68
6
ATOM 5697 O ALA C 276 51.614 129.755 18.804 1.00 60.70
8
ATOM 5698 N ALA C 277 53.155 128.882 17.395 1.00 41.08
7
ATOM 5699 CA ALA C 277 54.162 129.925 17.587 2.00 40.08
6
ATOM 5700 CB ALA C 277 55.221 129.831 16.495 1.00110.85
6
ATOM 5701 C ALA C 277 53.583 131.328 17.613 1.00 39.89
. 6
ATOM 5702 0 ALA C 277 53.900 132.111 18.506 1.00 38.90
8
ATOM 5703 N GLU C 278 52.739 131.644 16.633 1.00158.16
7
102

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ATOM 5704 CA GLU C 278 52.138 132.970 16.553 1.00159.25 6
ATOM 5705 CB GLU C 278 51.176 133.072 15.363 1.00206.63 6
ATOM 5706 CG GLU C 278 50.658 134.491 15.119 1.00208.87 6
ATOM 5707 CD GLU C 278 49.767 134.604 13.893 1.00208.87 6
ATOM 5708 OE1 GLU C 278 48.678 133.994 13.882 1.00208.87 8
ATOM 5709 OE2 GLU C 278 50.158 135.306 12.936 1.00208.87 8
ATOM 5710 C GLU C 278 51.398 133.302 17.834 1.00158.42 6
ATOM 5711 O GLU C 278 51.175 134.468 18.147 1.00158.85 8
ATOM 5712 N GLU C 279 51.007 132.278 18.576 1.00 95.46 7
ATOM 5713 CA GLU C 279 50.313 132.511 19.825 1.00 93.85 6
ATOM 5714 CB GLU C 279 48.905 131.927 19.770 1.00 94.99 6
ATOM 5715 CG GLU C 279 47.952 132.709 18.864 1.00 95.71 6
ATOM 5716 CD GLU C 279 47.892 134.192 19.207 1.00 96.88 6
ATOM 5717 OE1 GLU C 279 47.698 134.525 20.394 1.00 97.18 8
ATOM 5718 OE2 GLU C 279 48.031 135.033 18.292 1.00 98.81 8
ATOM 5719 C GLU C 279 51.115 131.910 20.960 1.00 92.63 6
ATOM 5720 O GLU C 279 50.578 131.279 21.865 1.00 93.45 8
ATOM 5721 N LYS C 280 52.420 132.124 20.885 1.00 48.25 7
ATOM 5722 CA LYS C 280 53.374 131.649 21.877 1.00 47.22 6
ATOM 5723 CB LYS C 280 53.812 130.213 21.571 1.00149.55 6
ATOM 5724 CG LYS C 280 52.707 129.162 21.590 1.00151.23 6
ATOM 5725 CD LYS C 280 52.316 128.745 23.004 1.00151.98 6
ATOM 5726 CE LYS C 280 51.303 127.596 22.974 1.00152.43 6
ATOM 5727 NZ LYS C 280 50.930 127.093 24.331 1.00151.40 7
ATOM 5728 C LYS C 280 54.570 132.581 21.718 1.00 46.41 6
ATOM 5729 O LYS C 280 55.041 133.204 22.673 1.00 45.83 8
ATOM 5730 N LEU C 281 55.052 132.666 20.484 1.00 94.79 7
ATOM 5731 CA LEU C 281 56.181 133.514 20.143 1.00 94.93 6
ATOM 5732 CB LEU C 281 56.928 132.932 18.942 1.00 69.65 6
ATOM 5733 CG LEU C 281 57.586 131.565 19.112 1.00 70.36 6
ATOM 5734 CD1 LEU C 281 58.050 131.071 17.764 1.00 69.69 6
ATOM 5735 CD2 LEU C 281 58.744 131.665 20.082 1.00 70.93 6
ATOM 5736 C LEU C 281 55.652 134.898 19.794 1.00 95.02 6
ATOM 5737 0 LEU C 281 54.462 135.169 19.946 1.00 94.94 8
ATOM 5738 N GLY C 282 56.543 135.764 19.322 1.00208.87 7
ATOM 5739 CA GLY C 282 56.153 137.113 18.954 1.00208.87 6
ATOM 5740 C GLY C 282 54.832 137.162 18.213 1.00208.87 6
ATOM 5741 0 GLY C 282 53.907 137.837 18.658 1.00208.87 8
ATOM 5742 N VAL C 283 54.756 136.438 17.096 1.00107.38 7
ATOM 5743 CA VAL C 283 53.568 136.359 16.239 1.00106.04 6
ATOM 5744 CB VAL C 283 52.587 137.562 16.457 1.00159.21 6
ATOM 5745 CG1 VAL C 283 51.944 137.990 15.142 1.00159.40 6
ATOM 5746 CG2 VAL C 283 51.490 137.167 17.433 1.00158.58 6
ATOM 5747 C VAL C 283 54.040 136.358 14.795 1.00105.26 6
ATOM 5748 O VAL C 283 55.190 136.684 14.520 1.00106.40 8
ATOM 5749 N GLY C 284 53.152 135.980 13.882 1.00 79.40 7
ATOM 5750 CA GLY C 284 53.495 135.955 12.473 1.00 78.56 6
ATOM 5751 C GLY C 284 54.516 134.892 12.116 1.00 78.47 6
ATOM 5752 O GLY C 284 55.423 134.604 12.898 1.00 78.70 8
ATOM 5753 N LEU C 285 54.376 134.312 10.928 1.00 96.55 7
ATOM 5754 CA LEU C 285 55.292 133.272 10.475 1.00 95.68 6
ATOM 5755 CB LEU C 285 55.217 132.086 11.426 1.00 67.63 6
ATOM 5756 CG LEU C 285 56.501 131.361 11.808 1.00 66.94 6
ATOM 5757 CD1 LEU C 285 56.192 130.527 13.029 1.00 66.94 6
ATOM 5758 CD2 LEU C 285 57.019 130.489 10.669 1.00 66.84 6
ATOM 5759 C LEU C 285 54.866 132.852 9.077 1.00 95.70 6
103

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WO 02/073192 PCT/US02/07535
ATOM 5760 O LEU C 285 54.099 133.5598.434 1.00 95.72 8
ATOM 5761 N SER C 286 55.351 131.7058.609 1.00140.80 7
ATOM 5762 CA SER C 286 55.001 131.2297.274 1.00140.03 6
ATOM 5763 CB SER C 286 55.741 132.0516.207 1.00102.60 6
ATOM 5764 OG SER C 286 55.555 133.4466.381 1.00101.88 8
ATOM 5765 C SER C 286 55.343 129.7537.072 1.00139.39 6
ATOM 5766 0 SER C 286 56.515 129.4016.961 1.00139.47 8
ATOM 5767 N GLY C 287 54.322 128.9007.028 1.00 95.77 7
ATOM 5768 CA GLY C 287 54.532 127.4746.805 1.00 95.78 6
ATOM 5769 C GLY C 287 55.666 126.7367.510 1.00 96.06 6
ATOM 5770 0 GLY C 287 55.409 125.9658.437 1.00 95.73 8
ATOM 5771 N ALA C 288 56.912 126.9457.071 1.00 94.57 7
ATOM 5772 CA ALA C 288 58.071 126.2687.671 1.00 94.17 6
ATOM 5773 CB ALA C 288 58.667 125.2986.676 1.00 50.77 6
ATOM 5774 C ALA C 288 59.189 127.1598.212 1.00 93.74 6
ATOM 5775 0 ALA C 288 59.444 128.2497.703 1.00 93.76 8
ATOM 5776 N THR C 289 59.862 126.6619.245 1.00 94.67 7
ATOM 5777 CA THR C 289 60.980 127.3629.869 1.00 95.18 6
ATOM 5778 CB THR C 289 61.803 126.43010.805 1.00 89.75 6
ATOM 5779 OG1 THR C 289 60.986 125.95511.885 1.00 89.80 8
ATOM 5780 CG2 THR C 289 62.991 127.17311.365 1.00 89.32 6
ATOM 5781 C THR C 289 61.898 127.7928.740 1.00 95.31 6
ATOM 5782 0 THR C 289 62.015 127.0877.741 1.00 95.64 8
ATOM 5783 N LEU C 290 62.548 128.9398.902 1.00 55.18 7
ATOM 5784 CA LEU C 290 63.465 129.4657.891 1.00 56.06 6
ATOM 5785 CB LEU C 290 64.782 128.6957.932 1.00 88.40 6
ATOM 5786 CG LEU C 290 65.811 129.0426.857 1.00 88.84 6
ATOM 5787 CD1 LEU C 290 66.107 130.527'6.877 1.00 89.92 6
ATOM 5788 CD2 LEU C 290 67.075 128.2437.104 1.00 88.80 6
ATOM 5789 C LEU C 290 62.903 129.4416.469 1.00 56.25 6
ATOM 5790 0 LEU C 290 61.992 128.6826.146 1.00 55.02 8
ATOM 5791 N VAL C 291 63.445 130.2985.619 1.00 81.17 7
ATOM 5792 CA VAL C 291 62.994 130.3654.243 1.00 83.23 6
ATOM 5793 CB VAL C 291 61.892 131.4374.046 1.00129.94 6
ATOM 5794 CG1 VAL C 291 61.529 131.5442.574 1.00129.79 6
ATOM 5795 CG2 VAL C 291 60.659 131.0804.862 1.00130.39 6
ATOM 5796 C VAL C 291 64.176 130.7043.359 1.00 84.36 6
ATOM 5797 0 VAL C 291 64.829 131.7343.536 1.00 83.65 8
ATOM 5798 N ALA C 292 64.449 129.8142.412 1.00196.81 7
ATOM 5799 CA ALA C 292 65.545 129.9911.475 1.00198.79 6
ATOM 5800 CB ALA C 292 66.540 128.8611.626 1.00125.71 6
ATOM 5801 C ALA C 292 64.990 130.0170.058 1.00200.14 6
ATOM 5802 O ALA C 292 63.800 130.267-0.148 1.00200.89 8
ATOM 5803 N PHE C 293 65.854 129.754-0.914 1.00 97.14 7
ATOM 5804 CA PHE C 293 65.458 129.752-2.316 1.00 98.47 6
ATOM 5805 CB PHE C 293 64.614 128.511-2.634 1.00208.87 6
ATOM 5806 CG PHE C 293 64.578 128.162-4.098 1.00208.87 6
ATOM 5807 CD1 PHE C 293 65.752 127.851-4.779 1.00208.87 6
ATOM 5808 CD2 PHE C 293 63.376 128.151-4.798 1.00208.87 6
ATOM 5809 CE1 PHE C 293 65.732 127.534-6.135 1.00208.87 6
ATOM 5810 CE2 PHE C 293 63.344 127.835-6.156 1.00208.87 6
ATOM 5811 CZ PHE C 293 64.526 127.527-6.824 1.00208.87 6
ATOM 5812 C PHE C 293 64.679 131.019-2.675 1.00 98.97 6
ATOM 5813 0 PHE C 293 64.020 131.083-3.715 1.00 99.42 8
ATOM 5814 N GLU C 294 64.748 132.020-1.802 1.00165.72 7
ATOM 5815 CA GLU C 294 64.078 133.294-2.039 1.00166.50 6
104

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ATOM 5816 CB GLU C 294 64.121 134.151-0.775 1.00133.84
ATOM 5817 CG GLU C 294 63.130 133.7420.283 1.00135.17
ATOM 5818 CD GLU C 294 61.717 134.128-0.086 1.00136.82
ATOM 5819 OE1 GLU C 294 61.464 135.338-0.270 1.00137.55
ATOM 5820 OE2 GLU C 294 60.859 133.226-0.192 1.00138.64
ATOM 5821 C GLU C 294 64.857 133.987-3.144 1.00166.56
ATOM 5822 O GLU C 294 64.294 134.476-4.126 1.00166.41
ATOM 5823 N ASP C 295 66.170 134.003-2.953 1.00135.96
ATOM 5824 CA ASP C 295 67.117 134.607-3.874 1.00135.93
ATOM 5825 CB ASP C 295 66.697 136.045-4.201 1.00 86.41
ATOM 5826 CG ASP C 295 66.565 136.913-2.967 1.00 85.80
ATOM 5827 OD1 ASP C 295 65.831 136.526-2.041 1.00 84.79
ATOM 5828 OD2 ASP C 295 67.200 137.985-2.927 1.00 86.23
ATOM 5829 C ASP C 295 68.477 134.582-3.177 1.00136.47
ATOM 5830 O ASP C 295 68.875 135.553-2.530 1.00136.80
ATOM 5831 N GLY C 296 69.173 133.455-3.293 1.00110.37
ATOM 5832 CA GLY C 296 70.470 133.326-2.655 1.00110.99
ATOM 5833 C GLY C 296 70.378 133.109-1.157 1.00111.59
ATOM 5834 0 GLY C 296 70.265 131.972-0.698 1.00112.40
ATOM 5835 N GLU C 297 70.414 134.197-0.391 1.00170.55
ATOM 5836 CA GLU C 297 70.353 134.1101.069 1.00170.60
ATOM 5837 CB GLU C 297 70.454 135.5041.701 1.00186.63
ATOM 5838 CG GLU C 297 69.344 136.4661.302 1.00188.22
ATOM 5839 CD GLU C 297 69.261 137.6762.219 1.00188.95
ATOM 5840 OE1 GLU C 297 70.310 138.3052.478 1.00189.46
ATOM 5841 OE2 GLU C 297 68.143 138.0042.673 1.00189.15
ATOM 5842 C GLU C 297 69.095 133.4261.586 1.00170.34
ATOM 5843 O GLU C 297 68.225 133.0220.818 1.00170.10
ATOM 5844 N PHE C 298 69.019 133.3092.906 1.00140.37
ATOM 5845 CA PHE C 298 67.893 132.6823.577 1:00140.50
ATOM 5846 CB PHE C 298 68.384 131.5304.457 1.00139.59
ATOM 5847 CG PHE C 298 68.795 130.3053.697 1.00140.10
ATOM 5848 CD1 PHE C 298 69.308 130.3992.411 1.00140.80
ATOM 5849 CD2 PHE C 298 68.701 129.0534.289 1.00139.57
ATOM 5850 CE1 PHE C 298 69.714 129.2621.721 1.00140.82
ATOM 5851 CE2 PHE C 298 69.103 127.9133.610 1.00139.07
ATOM 5852 CZ PHE C 298 69.613 128.0152.327 1.00139.85
ATOM 5853 C PHE C 298 67.176 133.6874.462 1.00140.52
ATOM 5854 O PHE C 298 67.815 134.4475.185 1.00141.28
ATOM 5855 N LYS C 299 65.850 133.6954.400 1.00 97.69
ATOM 5856 CA LYS C 299 65.072 134.5905.240 1.00 97.41
ATOM 5857 CB LYS C 299 64.040 135.3774.417 1.00101.03
ATOM 5858 CG LYS C 299 62.904 134.5493.816 1.00101.43
ATOM 5859 CD LYS C 299 61.770 135.4483.323 1.00102.35
ATOM 5860 CE LYS C 299 60.566 134.6372.870 1.00103.16
ATOM 5861 NZ LYS C 299 59.472 135.5102.363 1.00103.26
ATOM 5862 C LYS C 299 64.374 133.7366.296 1.00 96.80
ATOM 5863 O LYS C 299 63.288 133.2086.066 1.00 96.71
ATOM 5864 N ASP C 300 65.010 133.5917.454 1.00125.77
ATOM 5865 CA ASP C 300 64.447 132.7808.526 1.00124.41
ATOM 5866 CB ASP C 300 65.450 132.6199.667 1.00119.73
ATOM 5867 CG ASP C 300 65.503 133.83510.567 1.00120.65
ATOM 5868 OD1 ASP C 300 65.894 134.91610.083 1.00121.00
ATOM 5869 OD2 ASP C 300 65.144 133.70811.757 1.00120.80
ATOM 5870 C ASP C 300 63.174 133.3849.081 1.00123.28
ATOM 5871 0 ASP C 300 62.922 134.5808.948 1.00123.66
105

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ATOM 5872 N GLU C 301 62.376 132.538 9.723 1.00134.29
ATOM 5873 CA GLU C 301 61.129 132.969 10.312 1.00132.48
ATOM 5874 CB GLU C 301 59.957 132.201 9.694 1.00 85.74
ATOM 5875 CG GLU C 301 59.865 132.246 8.153 1.00 86.85
ATOM 5876 CD GLU C 301 59.436 133.597 7.591 1.00 87.33
ATOM 5877 OE1 GLU C 301 58.561 134.248 8.198 1.00 88.03
ATOM 5878 OE2 GLU C 301 59.957 133.999 6.529 1.00 87.91
ATOM 5879 C GLU C 301 61.224 132.687 11.816 1.00130.86
ATOM 5880 0 GLU C 301 61.342 133.612 12.621 1.00131.67
ATOM 5881 N VAL C 302 61.194 131.407 12.183 1.00150.57
ATOM 5882 CA VAL C 302 61.283 130.989 13.583 1.00147.82
ATOM 5883 CB VAL C 302 60.219 129.905 13.907 1.00130.83
ATOM 5884 CG1 VAL C 302 60.690 129.001 15.047 1.00131.76
ATOM 5885 CG2 VAL C 302 58.934 130.574 14.308 1.00131.06
ATOM 5886 C VAL C 302 62.662 130.445 13.945 1.00145.09
ATOM 5887 O VAL C 302 63.113 129.464 13.361 1.00145.82
ATOM 5888 N~ PHE C 303 63.320 131.081 14.911 1.00103.76
ATOM 5889 CA PHE C 303 64.638 130.642 15.351 1.00 99.80
ATOM 5890 CB PHE C 303 65.582 130.499 14.166 1.00 43.70
ATOM 5891 CG PHE C 30.3 65.991 129.084 23.898 1.00 41.90
ATOM 5892 CD1 PHE C 303 65.617 128.442 12.728 1.00 41.03
ATOM 5893 CD2 PHE C 303 66.751 128.386 14.819 1.00 41.62
ATOM 5894 CE1 PHE C 303 65.992 127.125 12.478 1.00 40.27
ATOM 5895 CE2 PHE C 303 67.131 127.066 14.574 1.00 42.22
ATOM 5896 CZ PHE C 303 66.749 126.437 13.399 1.00 41.12
ATOM 5897 C PHE C 303 65.257 131.580 16.377 1.00 98.71
ATOM 5898 O PHE C 303 65.676 131.146 17.452 1.00 99.30
ATOM 5899 N LEU C 304 65.342 132.861 16.034 1.00121.07
ATOM 5900 CA LEU C 304 65.881 133.866 16.947 1.00117.89
ATOM 5901 CB LEU C 304 66.549 135.010 16.1'721.00 58.33
ATOM 5902 CG LEU C 304 66.257 136.475 16.535 1.00 57.60
ATOM 5903 CD1 LEU C 304 67.456 137.347 16.186 1.00 57.31
ATOM 5904 CD2 LEU C 304 65.012 136.958 15.801 1.00 57.48
ATOM 5905 C LEU C 304 64.746 134.406 17.801 1.00116.09
ATOM 5906 0 LEU C 304 64.939 134.703 18.976 1.00116.63
ATOM 5907 N PRO C 305 63.542 134.543 17.215 1.00 79.40
ATOM 5908 CD PRO C 305 63.180 134.343 15.802 1.00 52.04
ATOM 5909 CA PRO C 305 62.390 135.052 17.962 1.00 77.80
ATOM 5910 CB PRO C 305 61.261 134.996 16.934 1.00 51.78
ATOM 5911 CG PRO C 305 61.974 135.230 15.662 1.00 52.39
ATOM 5912 C PRO C 305 62.115 134.171 19.175 1.00 76.42
ATOM 5913 0 PRO C 305 61.688 134.652 20.230 1.00 75.90
ATOM 5914 N THR C 306 62.353 132.875 19.013 1.00 64.70
ATOM 5915 CA THR C 306 62.153 131.944 20.103 1.00 62.16
ATOM 5916 CB THR C 306 62.114 130.496 19.588 1.00 37.62
ATOM 5917 OG1 THR C 306 60.961 129.845 20.128 1.00 37.49
ATOM 5918 CG2 THR C 306 63.355 129.717 20.003 1.00 36.49
ATOM 5919 C THR C 306 63.321 132.149 21.062 1.00 61.79
ATOM 5920 0 THR C 306 63.137 132.197 22.279 1.00 62.74
ATOM 5921 N LEU C 307 64.520 132.283 20.498 1.00 68.15
ATOM 5922 CA LEU C 307 65.735 132.514 21.276 1.00 66.39
ATOM 5923 CB LEU C 307 66.900 132.800 20.322 1.00 49.86
ATOM 5924 CG LEU C 307 68.341 132.691 20.827 1.00 48.12
ATOM 5925 CD1 LEU C 307 68.652 131.276 21.309 1.00 45.23
ATOM 5926 CD2 LEU C 307 69.268 133.098 19.693 1.00 47.43
ATOM 5927 C LEU C 307 65.405 133.739 22.121 1.00 66.43
106

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ATOM 5928 0 LEUC 307 65.789 133.850 23.285 1.00 66.27
ATOM 5929 N ARGC 308 64.663 134.647 21.499 1.00 50.53
ATOM 5930 CA ARGC 308 64.186 135.871 22.129 1.00 49.79
ATOM 5931 CB ARGC 308 63.410 136.697 21.090 1.00 71.35
ATOM 5932 CG ARGC 308 62.901 138.065 21.532 1.00 73.99
ATOM 5933 CD ARGC 308 62.560 138.925 20.304 1.00 75.25
ATOM 5934 NE ARGC 308 61.647 138.249 19.382 1.00 75.78
ATOM 5935 CZ ARGC 308 61.333 138.696 18.169 1.00 75.70
ATOM 5936 NH1 ARGC 308 61.855 139.825 17.713 1.00 75.04
ATOM 5937 NH2 ARGC 308 60.493 138.011 17.408 1.00 76.65
ATOM 5938 C ARGC 308 63.260 135.391 23.240 1.00 48.91
ATOM 5939 0 ARGC 308 63.627 135.416 24.420 1.00 48.85
ATOM 5940 N TYRC 309 62.074 134.923 22.841 1.00 59.73
ATOM 5941 CA TYRC 309 61.063 134.401 23.767 1.00 57.75
ATOM 5942 CB TYRC 309 59.986 133.625 22.997 1.00 46.74
ATOM 5943 CG TYRC 309 58.894 132.982 23.847 1.00 44.05
ATOM 5944 CD1 TYRC 309 57.800 133.719 24.291 1.00 44.13
ATOM 5945 CE1 TYRC 309 56.776 133.114 25.015 1.00 44.22
ATOM 5946 CD2 TYRC 309 58.938 131.622 24.161 1.00 42.45
ATOM 5947 CE2 TYRC 309 57.932 131.011 24.880 1.00 42.72
ATOM 5948 CZ TYRC 309 56.849 131.759 25.301 1.00 43.96
ATOM 5949 OH TYRC 309 55.813 131.147 25.968 1.00 44.56
ATOM 5950 C TYRC 309 61.698 133.487 24.805 1.00 56.45
ATOM 5951 0 TYRC 309 61.065 133.123 25.785 1.00 55.94
ATOM 5952 N LEUC 310 62.943 133.095 24.585 1.00 18.61
ATOM 5953 CA LEUC 310 63.594 132.257 25.554 1.00 19.45
ATOM 5954 CB LEUC 310 64.806 131.545 24.969 1.00 22.17
ATOM 5955 CG LEUC 310 65.500 130.689 26.043 1.00 20.12
ATOM 5956 CD1 LEUC 310 64.601 129.509 26.355 1.00 17.82
ATOM 5957 CD2 LEUC 310 66.891 130.218 25.601 1.00 18.38
ATOM 5958 C LEUC 310 64.056 133.133 26.699 1.00 21.90
ATOM 5959 0 LEUC 310 63.604 132.979 27.837 1.00 22.91
ATOM 5960 N PHEC 311 64.966 134.057 26.404 1.00 63.18
ATOM 5961 CA PHEC 311 65.502 134.952 27.430 1.00 64.52
ATOM 5962 CB PHEC 311 66.446 135.981 26.784 1.00 56.38
ATOM 5963 CG PHEC 311 67.695 135.373 26.181 1.00 56.52
ATOM 5964 CD1 PHEC 311 68.669 136.180 25.608 1.00 56.80
ATOM 5965 CD2 PHEC 311 67.897 133.998 26.186 1.00 56.67
ATOM 5966 CE1 PHEC 311 69.825 135.631 25.049 1.00 56.17
ATOM 5967 CE2 PHEC 311 69.046 133.446 25.630 1.00 56.99
ATOM 5968 CZ PHEC 311 70.011 134.268 25.060 1.00 56.37
ATOM 5969 C PHEC 311 64.378 135.643 28.208 1.00 65.71
ATOM 5970 0 PHEC 311 64.494 135.889 29.408 1.00 65.06
ATOM 5971 N ALAC 312 63.287 135.938 27.509 1.00109.29
ATOM 5972 CA ALAC 312 62.127 136.570 28.119 1.00110.75
ATOM 5973 CB ALAC 312 61.064 136.820 27.067 1.00 51.75
ATOM 5974 C ALAC 312 61.599 135.606 29.167 1.00111.67
ATOM. 5975 0 ALAC 312 60.890 135.989 30.093 1.00112.90
ATOM 5976 N LEUC 313 61.958 134.342 28.993 1.00 56.14
ATOM 5977 CA LEUC 313 61.558 133.269 29.883 1.00 55.56
ATOM 5978 CB LEUC 313 61.211 132.031 29.054 1.00 15.69
ATOM 5979 CG LEUC 313 61.013 130.634 29.656 1.00 14.79
ATOM 5980 CD1 LEUC 313 60.006 130.649 30.788 1.00 15.15
ATOM 5981 CD2 LEUC 313 60.543 129.704 28.560 1.00 13.87
ATOM 5982 C LEUC 313 62.729 132.989 30.800 1.00 56.68
ATOM 5983 0 LEUC 313 62.718 132.033 31.571 1.00 57.42
107

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ATOM 5984 N THR C 314 63.750 133.833 30.717 1.00 59.39
ATOM 5985 CA THR C 314 64.920 133.656 31.559 1.00 61.60
ATOM 5986 CB THR C 314 66.096 133.099 30.733 1.00 48.58
ATOM 5987 OG1 THR C 314 65.637 132.004 29.927 1.00 47.77
ATOM 5988 CG2 THR C 314 67.196 132.590 31.656 1.00 48.80
ATOM 5989 C THR C 314 65.315 134.972 32.246 1.00 63.97
ATOM 5990 O THR C 314 66.455 135.439 32.132 1.00 63.96
ATOM 5991 N ALA C 315 64.339 135.543 32.958 1.00 73.83
ATOM 5992 CA ALA C 315 64.444 136.793 33.722 1.00 74.62
ATOM 5993 CB ALA C 315 65.837 137.419 33.583 1.00 65.98
ATOM 5994 C ALA C 315 63.372 137.792 33.284 1.00 75.31
ATOM 5995 O ALA C 315 63.142 138.798 33.951 1.00 75.44
ATOM 5996 N GLY C 316 62.704 137.502 32.171 1.00 61.65
ATOM 5997 CA GLY C 316 61.675 138.396 31.672 1.00 63.94
ATOM 5998 C GLY C 316 60.316 138.208 ,32.318 1.00 65.80
ATOM 5999 O GLY C 316 60.215 137.699 33.440 1.00 65.97
ATOM 6000 N VAL C 317 59.270 138.625 31.604 1.00 71.99
ATOM 6001 CA VAL C 317 57.897 138.515 32.094 1.00 73.19
ATOM 6002 CB VAL C 317 57.187 139.884 32.089 1.00 50.58
ATOM 6003 CG1 VAL C 317 55.684 139.701 32.287 1.00 50.11
ATOM 6004 CG2 VAL C 317 57.753 140.758 33.199 1.00 50.53
ATOM 6005 C VAL C 317 57.003 137.513 31.356 1.00 75.00
ATOM 6006 O VAL C 317 56.214 136.816 31.988 1.00 75.45
ATOM 6007 N PRO C 318 57.107 137.421 30.016 1.00149.53
ATOM 6008 CD PRO C 318 58.077 138.026 29.086 1.00 68.35
ATOM 6009 CA PRO C 318 56.246 136.457 29.315 1.00150.81
ATOM 6010 CB PRO C 318 56.628 136.657 27.849 1.00 68.99
ATOM 6011 CG PRO C 318 58.067 137.042 27.932 1.00 68.35
ATOM 6012 C PRO C 318 56.483 135.027 29.801 1.00152.05
ATOM 6013 O PRO C 318 55.666 134.136 29.583 1.00151.58
ATOM 6014 N GLY C 319 57.620 134.840 30.465 1.00 73.22
ATOM 6015 CA GLY C 319 58.015 133.559 31.023 1.00 74.96
ATOM 6016 C GLY C 319 57.383 132.312 30.452 1.00 76.68
ATOM 6017 O GLY C 319 57.790 131.826 29.400 1.00 76.07
ATOM 6018 N HIS C 320 56.382 131.794 31.152 1.00 84.00
ATOM 6019 CA HIS C 320 55.699 130.575 30.734 1.00 86.17
ATOM 6020 CB HIS C 320 54.877 130.812 29.460 1.00158.67
ATOM 6021 CG HIS C 320 53.751 131.785 29.638 1.00159.50
ATOM 6022 CD2 HIS C 320 53.332 132.816 28.866 1.00159.65
ATOM 6023 ND1 HIS C 320 52.900 131.749 30.722 1.00159.24
ATOM 6024 CE1 HIS C 320 52.008 132.716 30.611 1.00159.88
ATOM 6025 NE2 HIS C 320 52.248 133.379 29.494 1.00159.94
ATOM 6026 C HIS C 320 56.719 129.458 30.510 1.00 86.95
ATOM 6027 O HIS C 320 57.403 129.405 29.488 1.00 86.53
ATOM 6028 N GLU C 321 56.829 128.577 31.495 1.00135.10
ATOM 6029 CA GLU C 321 57.757 127.466 31.414 1.00136.56
ATOM 6030 CB GLU C 321 58.179 127.028 32.817 1.00165.69
ATOM 6031' CG GLU C 321 58.904 128.108 33.613 1.00167.45
ATOM 6032 CD GLU C 321 58.115 128.592 34.822 1.00168.27
ATOM 6033 OE1 GLU C 321 56.993 129.110 34.642 1.00169.04
ATOM 6034 OE2 GLU C 321 58.621 128.457 35.956 1.00167.77
ATOM 6035 C GLU C 321 57.072 126.320 30.692 1.00137.60
ATOM 6036 O GLU C 321 57.513 125.177 30.783 1.00138.15
ATOM 6037 N VAL C 322 55.993 126.635 29.977 1.00 99.38
ATOM 6038 CA VAL C 322 55.236 125.628 29.233 1.00100.56
ATOM 6039 CB VAL C 322 54.463 126.252 28.026 1.00100.02
108

CA 02440409 2003-09-09
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ATOM 6040 CG1 VAL C322 55.386 127.09927.186 1.00100.63
6
ATOM 6041 CG2 VAL C322 53.849 125.15527.168 1.00 99.26
6
ATOM 6042 C VAL C322 56.139 124.51228.726 1.00101.41
6
ATOM 6043 0 VAL C322 57.015 124.73027.890 1.00101.84
8
ATOM 6044 N ALA C323 55.922 123.31729.260 1.00115.83
7
ATOM 6045 CA ALA C323 56.704 122.15128.881 1.00116.94
6
ATOM 6046 CB ALA C323 57.288 121.49830.132 1.00101.48
6
ATOM 6047 C ALA C323 55.798 121.16928.143 1.00117.29
6
ATOM 6048 0 ALA C323 54.783 121.57127.572 1.00117.57
8
ATOM 6049 N ASP C324 56.164 119.89028.133 1.00 90.38
7
ATOM 6050 CA ASP C324 55.321 118.89827.498 1.00 90.03
6
ATOM 6051 CB ASP C324 56.020 117.53527.468 1.00208.87
6
ATOM 6052 CG ASP C324 56.492 117.08528.837 1.00208.87
6
ATOM 6053 OD1 ASP C324 57.291 117.81329.465 1.00208.87
8
ATOM 6054 OD2 ASP C324 56.065 115.99729.279 1.00208.87
8
ATOM 6055 C ASP C324 54.108 118.87528.414 1.00 88.37
6
ATOM 6056 0 ASP C324 53.586 117.82228.765 1.00 87.10
8
ATOM 6057 N ALA C325 53.698 120.08128.799 1.00129.49
7
ATOM 6058 CA ALA C325 52.588 120.36729.689 1.00128.97
6
ATOM.6059 CB ALA C325 52.037 121.74629.362 1.00 37.90
6
ATOM 6060 C ALA C325 51.474 119.32929.703 1.00128.58
6
ATOM 6061 0 ALA C325 50.558 119.40330.520 1.00129.16
8
ATOM 6062 N ASP C326 51.550 118.36428.798 1.00 84.98
7
ATOM 6063 CA ASP C326 50.574 117.29628.732 1.00 83.82
6
ATOM 6064 CB ASP C326 50.641 116.45330.004 1.00 99.04
6
ATOM 6065 CG ASP C326 52.050 115.99230.321 1.00 99.14
6
ATOM 6066 OD1 ASP C326 52.692 115.37429.444 1.00 98.26
8
ATOM 6067 OD2 ASP C326 52.516 116.24431.451 1.00 99.68
8
ATOM 6068 C ASP C326 49.179 117.85028.542 1.00 82.28
6
ATOM 6069 0 ASP C326 48.217 117.09928.405 1.00 83.20
8
ATOM 6070 N HIS C327 49.065 119.17028.552 1.00131.67
7
ATOM 6071 CA HIS C327 47.772 119.78028.340 1.00129.72
6
ATOM 6072 CB HIS C327 47.902 121.30228.263 1.00 67.84
6
ATOM 6073 CG HIS C327 46.595 122.01428.079 1.00 66.29
6
ATOM 6074 CD2 HIS C327 46.013 122.53826.974 1.00 66.15
6
ATOM 6075 ND1 HIS C327 45.706 122.22229.111 1.00 65.92
7
ATOM 6076 CE1 HIS C327 44.633 122.83928.651 1.00 66.32
6
ATOM 6077 NE2 HIS C327 44.794 123.04327.357 1.00 66.58
7
ATOM 6078 C HIS C327 47.406 119.22926.978 1.00127.76
6
ATOM 6079 0 HIS C327 48.203 119.33026.050 1.00127.72
8
ATOM 6080 N LEU C328 46.235 118.61426.850 1.00 89.73
7
ATOM 6081 CA LEU C328 45.844 118.08625.553 1.00 85.71
6
ATOM 6082 CB LEU C328 44.593 117.232.25.656 1.00 33.75
6
ATOM 6083 CG LEU C328 44.896 115.76825.924 1.00 32.84
6
ATOM 6084 CD1 LEU C328 45.556 115.63427.290 1.00 30.73
6
ATOM 6085 CD2 LEU C328 43.613 114.95625.846 1.00 34.53
6
ATOM 6086 C LEU C328 45.628 119.20024.547 1.00 83.13
6
ATOM 6087 O LEU C328 44.682 119.18523.766 1.00 83.74
8
ATOM 6088 N GLY C329 46.511 120.18524.611 1.00 95.19
7
ATOM 6089 CA GLY C329 46.493 121.29523.690 1.00 91.47
6
ATOM 6090 C GLY C329 47.828 121.08823.015 1.00 88.66
6
ATOM 6091 O GLY C329 48.204 121.80022.087 1.00 90.13
8
ATOM 6092 N ASN C330 48.546 120.08123.511 1.00 70.19
7
ATOM 6093 CA ASN C330 49.861 119.70922.999 1.00 65.88
6
ATOM 6094 _ ASN C330 50.958 120.22123.938 1.00 42.25
CB 6
ATOM 6095 CG ASN C330 50.787 121.68624.286 1.00 39.89
6
109

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 6096 OD1 ASNC 330 50.329 122.468 23.460 1.00 37.44
ATOM 6097 ND2 ASNC 330 51.167 122.065 25.508 1.00 37.85
ATOM 6098 C ASNC 330 49.995 118.190 22.841 1.00 63.95
ATOM 6099 O ASNC 330 51.087 117.680 22.605 1.00 65.25
ATOM 6100 N ARGC 331 48.879 117.476 22.977 1.00 31.86
ATOM 6101 CA ARGC 331 48.865 116.024 22.846 1.00 28.53
ATOM 6102 CB ARGC 331 48.153 115.381 24.030 1.00 42.69
ATOM 6103 CG ARGC 331 49.024 115.241 25.250 1.00 42.49
ATOM 6104 CD ARGC 331 49.702 113.896 25.297 1.00 41.46
ATOM 6105 NE ARGC 331 50.969 113.996 26.005 1.00 41.16
ATOM 6106 CZ ARGC 331 51.525 113.016 26.714 1.00 41.94
ATOM 6107 NH1 ARGC 331 50.928 111.829 26.831 1.00 41.98
ATOM 6108 NH2 ARGC 331 52.689 113.234 27.317 1.00 42.25
ATOM 6109 C ARGC 331 48.168 115.619 21.579 1.00 27.55
ATOM 6110 O ARGC 331 46.963 115.441 21.547 1.00 26.44
ATOM 6111 N ARGC 332 48.952 115.471 20.530 1.00 63.72
ATOM 6112 CA ARGC 332 48.440 115.079 19.235 1.00 63.94
ATOM 6113 CB ARGC 332 49.574 115.223 18.210 1.00 73.34
ATOM 6114 CG ARGC 332 49.251 114.856 16.779 1.00 75.34
ATOM 6115 CD ARGC 332 48.450 115.921 16.106 1.00 78.35
ATOM 6116 NE ARGC 332 48.485 115.736 14.666 1.00 83.95
ATOM 6117 CZ ARGC 332 47.961 116.591 13.796 1.00 87.69
ATOM 6118 NH1 ARGC 332 47.355 117.694 14.222 1.00 88.64
ATOM 6119 NH2 ARGC 332 48.058 116.352 12.494 1.00 90.07
ATOM 6120 C ARGC 332 47.948 113.628 19.296 1.00 62.87
ATOM 6121 O ARGC 332 48.369 112.851 20.157 1.00 61.84
ATOM 6122 N ILEC 333 47.040 113.279 18.386 1.00 62.90
ATOM 6123 CA ILEC 333 46.515 111.922 18.289 1.00 60.22
ATOM 6124 CB ILEC 333 45.088 111.824 18.812 1.00 18.02
ATOM 6125 CG2 ILEC 333 44.509 110.467 18.476 1.00 16.60
ATOM 6126 CG1 ILEC 333 45.096 112.057 20.326 1.00 18.58
ATOM 6127 CD1 ILEC 333 43.788 111.693 21.050 1.00 20.84
ATOM 6128 C ILEC 333 46.541 111.423 16.848 1.00 60.00
ATOM 6129 O ILEC 333 45.592 111.616 16.089 1.00 60.88
ATOM 6130 N ARGC 334 47.645 110.788 16.476 1.00 40.82
ATOM 6131 CA ARGC 334 47.804 110.249 15.140 1.00 39.79
ATOM 6132 CB ARGC 334 49.236 109.762 14.940 1.00 48.95
ATOM 6133 CG ARGC 334 50.248 110.869 14.856 1.00 50.53
ATOM 6134 CD ARGC 334 51.576 110.327 14.389 1.00 53.11
ATOM 6135 NE ARGC 334 52.108 109.330 15.310 1.00 55.57
ATOM 6136 CZ ARGC 334 53.243 108.669 15.104 1.00 58.22
ATOM 6137 NH1 ARGC 334 53.954 108.905 14.007 1.00 59.53
ATOM 6138 NH2 ARGC 334 53.676 107.776 15.986 1.00 59.09
ATOM 6139 C ARGC 334 46.833 109.104 14.857 1.00 38.72
ATOM 6140 O ARGC 334 47.088 107.950 15.206 1.00 38.77
ATOM 6141 N THRC 335 45.719 109.436 14.220 1.00 27.72
ATOM 6142 CA THRC 335 44.711 108.451 13.858 1.00 26.86
ATOM 6143 CB THRC 335 43.524 109.147 13.142 1.00 52.92
ATOM 6144 OG1 THRC 335 43.737 110.568 13.137 1.00 52.44
ATOM 6145 CG2 THRC 335 42.214 108.858 13.862 1.00 53.20
ATOM 6146 C THRC 335 45.374 107.445 12.909 1.00 26.26
ATOM 6147 0 THRC 335 46.487 107.680 12.439 1.00 26.38
ATOM 6148 N VALC 336 44.708 106.328 12.625 1.00 19.30
ATOM 6149 CA VALC 336 45.295 105.333 11.724 1.00 19.05
ATOM 6150 CB VALC 336 44.296 104.234 11.295 1.00 19.08
ATOM 6151 CG1 VALC 336 45.051 103.070 10.675 1.00 17.32
110

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ATOM 6152 CG2 VAL C 336 43.466 103.78112.471 1.00 21.04
6
ATOM 6153 C VAL C 336 45.732 106.03710.454 1.00 19.76
6
ATOM 6154 O VAL C 336 46.918 106.15610.190 1.00 19.67
8
ATOM 6155 N GLY C 337 44.758 106.5119.681 1.00 39.74
7
ATOM 6156 CA GLY C 337 45.048 107.1818.424 1.00 41.07
6
ATOM 6157 C GLY C 337 46.305 108.0218.456 1.00 41.88
6
ATOM 6158 0 GLY C 337 46.993 108.1697.441 1.00 41.06
8
ATOM 6159 N GLU C 338 46.589 108.5809.629 1.00 53.46
7
ATOM 6160 CA GLU C 338 47.764 109.4089.828 1.00 55.83
6
ATOM 6161 CB GLU C 338 47.595 110.22811.117 1.00 55.93
6
ATOM 6162 CG GLU C 338 48.522 111.44611.269 1.00 57.68
6
ATOM 6163 CD GLU C 338 48.070 112.41612.375 1.00 59.12
6
ATOM 6164 OE1 GLU C 338 48.862 113.31312.738 1.00 59.71
8
ATOM 6165 OE2 GLU C 338 46.927 112.29312.877 1.00 58.33
8
ATOM 6166 C GLU C 338 48.970 108.4669.901 1.00 56.52
6
ATOM 6167 O GLU C 338 49.940 108.6429.164 1.00 57.30
8
ATOM 6168 N LEU C 339 48.896 107.45610.768 1.00 46.17
7
ATOM 6169 CA LEU C 339 49.972 106.47010.919 1.00 45.76
6
ATOM 6170 CB LEU C 339 49.568 105.39811.912 1.00 29.77
6
ATOM 6171 CG LEU C 339 49.259 105.82613.334 1.00 28.34
6
ATOM 6172 CD1 LEU C 339 48.193 104.90113.876 1.00 27.80
6
ATOM 6173 CD2 LEU C 339 50.520 105.80414.183 1.00 26.92
6
ATOM 6174 C LEU C 339 50.247 105.7859.593 1.00 46.77
6
ATOM 6175 O LEU C 339 51.394 105.6609.172 1.00 47.55
8
ATOM 6176 N MET C 340 49.170 105.3198.966 1.00 40.41
7
ATOM 6177 CA MET C 340 49.213 104.6427.675 1.00 41.73
6
ATOM 6178 CB MET C 340 47.815 104.1027.357 1.00 33.54
6
ATOM 6179 CG MET C 340 47.726 103.2816.098 1.00 34.95
6
ATOM 6180 SD MET C 340 47.982 104.2554.606 1.00 37.58
16
ATOM 6181 CE MET C 340 46.303 104.5754.124 1.00 37.44
6
ATOM 6182 C MET C 340 49.685 105.6246.585 1.00 42.70
6
ATOM 6183 O MET C 340 ' 50.313 105.2375.590 1.00 41.52
8
ATOM 6184 N ALA C 341 49,379 106.9026.782 1:00 58.39
7
ATOM 6185 CA ALA C 341 49.783 107.9225.832 1.00 59.26
6
ATOM 6186 CB ALA C 341 49.214 109.2756.234 1.00 54.63
6
ATOM 6187 C ALA C 341 51.302 107.9755.808 1.00 59.78
6
ATOM 6188 O ALA C 341 51.918 107.6004.819 1.00 60.61
8
ATOM 6189 N ASP C 342 51.903 108.4256.905 1.00 54.65
7
ATOM 6190 CA ASP C 342 53.360 108.5307.002 1.00 55.34
6
ATOM 6191 CB ASP C 342 53.785 108.7508.459 1.00 64.26
6
ATOM 6192 CG ASP C 342 53.416 110.1248.978 1.00 65.24
6
ATOM , 6193OD1 ASP C 342 53.659 111.1258.266 1.00 66.05
8
ATOM 6194 OD2 ASP C 342 52.896 110.20110.112 1.00 65.46
8
ATOM 6195 C ASP C 342 54.121 107.3206.447 1.00 55.56
6
ATOM 6196 0 ASP C 342 55.191 107.4675.850 1.00 54.83
8
ATOM 6197 N GLN C 343 53.573 106.1286.656 1.00 49.93
7
ATOM 6198 CA GLN C 343 54.217 104.9166.183 1.00 49.77
6
ATOM 6199 CB GLN C 343 53.488 103.6946.731 1.00 55.92
6
ATOM 6200 CG GLN C 343 54.392 102.4896.863 1.00 56.26
6
ATOM 6201 CD GLN C 343 55.600 102.7737.735 1.00 55.71
6
ATOM 6202 OE1 GLN C 343 55.526 102.7438.965 1.00 54.57
8
ATOM 6203 NE2 GLN C 343 56.722 103.0727.094 1.00 55.01
7
ATOM 6204 C GLN C 343 54.201 104.9034.663 1.00 50.19
6
ATOM 6205 O GLN C 343 55.062 104.2994.019 1.00 50.28
8
ATOM 6206 N PHE C 344 53.201 105.5754.102 1.00 68.25
7
ATOM 6207 CA PHE C 344 53.042 105.6952.657 1.00 68.99
6
111

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 6208 CB PHE C 344 51.628 106.199 2.331 1.00 53.22 6
ATOM 6209 CG PHE C 344 51.092 105.758 0.986 1.00 52.79 6
ATOM 6210 CD1 PHE C 344 49.781 106.061 0.620 1.00 53.28 6
ATOM 6211 CD2 PHE C 344 51.870 105.031 0.106 1.00 52.38 6
ATOM 6212 CE1 PHE C 344 49.257 105.648 =0.589 1.00 53.18 6
ATOM 6213 CE2 PHE C 344 51.350 104.614 -1.107 1.00 52.97 6
ATOM 6214 CZ PHE C 344 50.039 104.925 -1.452 1.00 53.08 6
ATOM 6215 C PHE C 344 54.088 106.735 2.263 1.00 69.69 6
ATOM 6216 O PHE C 344 54.635 106.701 1.165 1.00 70.88 8
ATOM 6217 N ARG C 345 54.359 107.663 3.177 1.00 46.04 7
ATOM 6218 CA ARG C 345 55.365 108.684 2.937 1.00 46.35 6
ATOM 6219 CB ARG C 345 55.367 109.716 4.068 1.00 60.38 6
ATOM 6220 CG ARG C 345 54.080 110.523 4.148 1.00 61.90 6
ATOM 6221 CD ARG C 345 54.147 111.609 5.213 1.00 62.12 6,
ATOM 6222 NE ARG C 345 52.979 112.495 5.186 1.00 61.97 7
ATOM 6223 CZ ARG C 345 51.801 112.224 5.747 1.00 61.97 6
ATOM 6224 NH1 ARG C 345 51.617 111.081 6.396 1.00 61.31 7
ATOM 6225 NH2 ARG C 345 50.800 113.096 5.656 1.00 62.32 7
ATOM 6226 C ARG C 345 56.701 107.954 2.879 1.00 46.29 6
ATOM 6227 O ARG C 345 57.502 108.172,1.974 1.00 45.82 8
ATOM 6228 N VAL C 346 56.917 107.059 3.837 1.00 59.02 7
ATOM 6229 CA VAL C 346 58.148 106.272 3.909 1.00 59.39 6
ATOM 6230 CB VAL C 346 58.072 105.227 5.042 1.00106.13 6
ATOM 6231 CG1 VAL C 346 59.400 104.491 5.167 1.00106.98 6
ATOM 6232 CG2 VAL C 346 57.705 105.908 6.348 1.00106.03 6
ATOM 6233 C VAL C 346' 58.418 105.533 2.603 1.00 59.04 6
ATOM 6234 O VAL C 346 59.521 105.588 2.059 1.00 58.47 8
ATOM 6235 N GLY C 347 57.402 104.830 2.113 1.00 36.64 7
ATOM 6236 CA GLY C 347 57.550 104.086 0.880 1.00 38.67 6
ATOM 6237 C GLY C 347 58.151 104.955 -0.197 1.00 40.04 6
ATOM 6238 O GLY C 347 59.142 104.591 -0.818 1.00 38.54 8
ATOM 6239 N LEU C 348 57.550 106.116 -0.410 1.00 81.14 7
ATOM 6240 CA LEU C 348 58.032 107.041 -1.415 1.00 83.72 6.
ATOM 6241 CB LEU C 348 57.045 108.198 -1.557 1.00 23.40 6
ATOM 6242 CG LEU C 348 55.937 107.959 -2.591 1.00 21.23 6
ATOM 6243 CD1 LEU C 348 55.523 106.507 -2.605 1.00 21.40 6
ATOM 6244 CD2 LEU C 348 54.750 108.847 -2.277 1.00 20.29 6
ATOM 6245 C LEU C 348 59.430 107.551 -1.088 1.00 87.02 6
ATOM 6246 0 LEU C 348 60.176 107.943 -1.982 1.00 87.49 8
ATOM 6247 N ALA C 349 59.794 107.536 0.190 1.00205.10 7
ATOM 6248 CA ALA C 349 61.122 107.987 0.597 1.00208.87 6
ATOM 6249 CB ALA C 349 61.181 108.163 2.107 1.00160.69 6
ATOM 6250 C ALA C 349 62.125 106.933 0.157 1.00208.87 6
ATOM 6251 0 ALA C 349 63.265 106.903 0.622 1.00208.87 8
ATOM 6252 N ARG C 350 61.680 106.069 -0.751 1.00 62.51 7
ATOM 6253 CA ARG C 350 62.495 104.983 -1.264 1.00 63.53 6
ATOM 6254 CB ARG C 350 62.248 103.770 -0.392 1.00 74.24 6
ATOM 6255 CG ARG C 350 62.658 104.075 1.026 1.00 75.44 6
ATOM 6256 CD ARG C 350 61.948 103.257 2.077 1.00 75.95 6
ATOM 6257 NE ARG C 350 62.617 103.445 3.360 1.00 76.40 7
ATOM 6258 CZ ARG C 350 62.274 102.835 4.485 1.00 77.84 6
ATOM 6259 NH1 ARG C 350 61.251 101.987 4.500 1.00 78.67 7
ATOM 6260 NH2.ARG C 350 62.971 103.061 5.589 1.00 79.33 7
ATOM 6261 C ARG C 350 62.158 104.712 -2.727 1.00 64.82 6
ATOM 6262 0 ARG C 350 63.020 104.347 -3.525 1.00 65.28 8
ATOM 6263 N LEU C 351 60.891 104.890 -3.072 1.00 63.44 7
112

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ATOM 6264 CA LEU C 351 60.462 104.716-4.444 1.00 64.60
6
ATOM 6265 CB LEU C 351 59.025 105.189-4.612 1.00 69.32
6
ATOM 6266 CG LEU C 351 58.740 105.890-5.937 1.00 69.81
6
ATOM 6267 CD1 LEU C 351 58.831 104.902-7.077 1.00 70.58
6
ATOM 6268 CD2 LEU C 351 57.374 106.517-5.887 1.00 69.70
6
ATOM 6269 C LEU C 351 61.379 105.643-5.196 1.00 65.69
6
ATOM 6270 0 LEU C 351 62.157 105.214-6.032 1.00 66.02
8
ATOM 6271 N ALA C 352 61.290 106.924-4.856 1.00 97.21
7
ATOM 6272 CA ALA C 352 62.096 107.968-5.477 1.00 98.34
6
ATOM 6273 CB ALA C 352 61.961 109.260-4.693 1.00107.71
6
ATOM 6274 C ALA C 352 63.566 107.581-5.598 1.00 98.88
6
ATOM 6275 O ALA C 352 64.119 107.579-6.698 1.00 99.53
8
ATOM 6276 N ARG C 353 64.208 107.271-4.473 1.00 45.94
7
ATOM 6277 CA ARG C 353 65.609 106.871-4.514 1.00 47.38
6
ATOM 6278 CB ARG C 353 65.973 106.085-3.254 1.00115.76
6
ATOM 6279 CG ARG C 353 65.775 106.841-1.955 1.00117.15
6
ATOM 6280 CD ARG C 353 66.825 107.914-1.782 1.00118.82
6
ATOM 6281 NE ARG C 353 66.625 108.662-0.546 1.00120.11
7
ATOM 6282 CZ ARG C 353 67.444 109.613-0.108 1.00121.05
6
ATOM 6283 NH1 ARG C 353 6$.529 109.935-0.805 1.00120.67
7
ATOM 6284 NH2 ARG C 353 67.177 110.2451.028 1.00121.41
7
ATOM 6285 C ARG C 353 65.763 105.972-5.742 1.00 48.30
6
ATOM 6286 0 ARG C 353 66.610 106.199-6.605 1.00 49.16
8
ATOM 6287 N GLY C 354 64.908 104.959-5.820 1.00160.51
7
ATOM 6288 CA GLY C 354 64.955 104.049-6.944 1.00160.56
6
ATOM 6289 C GLY C 354 64.848 104.787-8.263 1.00160.66
6
ATOM 6290 O GLY C 354 65.679 104.601-9.152 1.00161.53
8
ATOM 6291 N VAL C 355 63.835 105.638-8.395 1.00 80.66
7
ATOM 6292 CA VAL C 355 63.653 106.371-9.633 1.00 80.30
6
ATOM 6293 CB VAL C 355 62.332 107.204-9.622 1.00 45.69
6
ATOM 6294 CG1 VAL C 355 62.416 108.360-8.651 1.00 45.97
6
ATOM 6295 CG2 VAL C 355 62.024 107.704-11.027 1.00 45.21
6
ATOM 6296 C VAL C 355 64.861 107.258-9.939 1.00 80.64
6
ATOM 6297 O VAL C 355 65.468 107.114-10.998 1.00 81.25
8
ATOM 6298 N ARG C 356 65.226 108.152-9.022 1.00 80.64
7
ATOM 6299 CA ARG C 356 66.383 109.022-9.238 1.00 80.31
6
ATOM 6300 CB ARG C 356 66.842 109.659-7.923 1.00101.69
6
ATOM 6301 CG ARG C 356 68.368 109.649-7.714 1.00101.76
6
ATOM 6302 CD ARG C 356 69.130 110.592-8.662 1.00102.00
6
ATOM 6303 NE ARG C 356 70.579 110.374-8.601 1.00101.48
7
ATOM 6304 CZ ARG C 356 71.496 111.201-9.105 1.00101.01
6
ATOM 6305 NH1 ARG C 356 71.127 112.321-9.715 1.00100.84
7
ATOM 6306 NH2 ARG C 356 72.787 110.906-8.997 1.00101.02
7
ATOM 6307 C ARG C 356 67.522 108.191-9.800 1.00 80.38
6
ATOM 6308 O ARG C 356 68.235 108.631-10.702 1.00 79.87
8
ATOM 6309 N GLU C 357 67.685 106.993-9.241 1.00 70.79
7
ATOM 6310 CA GLU C 357 68.723 106.060-9.654 1.00 71.20
6
ATOM 6311 CB GLU C 357 68.626 104.772-8.836 1.00197.19
6
ATOM 6312 CG GLU C 357 69.548 103.661-9.312 1.00199.52
6
ATOM 6313 CD GLU C 357 71.008 103.958-9.048 1.00200.44
6
ATOM 6314 OE1 GLU C 357 71.479 105.043-9.451 1.00202.08
8
ATOM 6315 OE2 GLU C 357 71.687 103.101-8.441 1.00200.31
8
ATOM 6316 C GLU C 357 68.588 105.724-11.128 1.00 70.90
6
ATOM 6317 O GLU C 357 69.535 105.887-11.891 1.00 70.88
8
ATOM 6318 N ARG C 358 67.405 105.257-11.521 1.00 98.92
7
ATOM 6319 CA ARG C 358 67.143 104.884-12.911 1.00 99.88
6
113

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ATOM 6320 CB ARG C 358 65.758 104.240-13.040 1.00 90.72
6
ATOM 6321 CG ARG C 358 ~ 65.413 103.224-11.957 1.00 90.05
6
ATOM 6322 CD ARG C 358 66.334 102.006-11.935 1.00 88.23
6
ATOM 6323 NE ARG C 358 65.958 101.101-10.849 1.00 86.36
7
ATOM 6324 CZ ARG C 358 66.613 99.992 -10.520 1.00 85.41
6
ATOM 6325 NH1 ARG C 358 67.696 99.623 -11.189 1.00 84.88
7
ATOM 6326 NH2 ARG C 358 66.181 99.250 -9.513 1.00 85.19
7
ATOM 6327 C ARG C 358 67.229 106.086-13.855 1.00101.13
6
ATOM 6328 0 ARG C 358 67.926 106.033-14.871 1.00101.33
8
ATOM 6329 N MET C 359 66.508 107.155-13.521 1.00 78.58
7
ATOM 6330 CA MET C 359 66.510 108.377-14.326 1.00 80.42
6
ATOM 6331 CB MET C 359 66.186 109.607-13.457 1.00 98.08
6
ATOM 6332 CG MET C 359 64.756 109.687-12.898 1.00100.34
6
ATOM 6333 SD MET C 359 63.524 110.434-14.000 1.00101.54
16
ATOM 6334 CE MET C 359 63.522 112.080-13.421 1.00101.52
6
ATOM 6335 C MET C 359 67.886 108.561-14.951 1.00 81.29
6
ATOM 6336 O MET C 359 68.002 108.834-16.141 1.00 81.42
8
ATOM 6337 N VAL C 360 68.926 108.397-14.138 1.00137.54
7
ATOM 6338 CA VAL C 360 70.300 108.547-14.600 1.00139.21
6
ATOM 6339 CB VAL C 360 71.267 108.621-13.408 1.00 61.42
6
ATOM 6340 CG1 VAL C 360 72.711 108.678-13.883 1.00 61.29
6
ATOM 6341 CG2 VAL C 360 70.942 109.837-12.591 1.00 61.94
6
ATOM 6342 C VAL C 360 70.726 107.419-15.527 1.00140.74
6
ATOM 6343 0 VAL C 360 70.462 107.475-16.725 1.00141.23
8
ATOM 6344 N MET C 361 71.380 106.402-14.968 1.00105.12
7
ATOM 6345 CA MET C 361 71.857 105.263-15.743 1.00106.42
6
ATOM 6346 CB MET C 361 71.439 103.927-15.114 1.00110.40
6
ATOM 6347 CG MET C 361 71.938 103.629-13.716 1.00112.79
6
ATOM 6348 SD MET C 361 71.479 101.941-13.236 1.00113.05
16
ATOM 6349 CE MET C 361 69.714 102.024-13.313 1.00113.30
6
ATOM 6350 C MET C 361 71.259 105.295-17.128 1.00106.56
6
ATOM 6351 O MET C 361 71.868 105.790-18.076 1.00107.71
8
ATOM 6352 N GLY C 362 70.036 104.776-17.205 1.00 77.49
7
ATOM 6353 CA GLY C 362 69.305 104.676-18.453 1.00 77.57
6
ATOM 6354 C GLY C 362 69.388 105.847-19.402 1.00 77.61
6
ATOM 6355 0 GLY C 362 69.930 106.897-19.070 1.00 78.30
8
ATOM 6356 N SER C 363 68.842 105.650-20.597 1.00117.73
7
ATOM 6357 CA SER C 363 68.822 106.685-21.620 1.00118.18
6
ATOM 6358 CB SER C 363 68.919 106.057-23.015 1.00165.58
6
ATOM 6359 OG SER C 363 68.963 107.047-24.028 1.00165.87
8
ATOM 6360 C SER C 363 67.511 107.450-21.487 1.00118.23
6
ATOM 6361 0 SER C 363 66.501 106.897-21.058 1.00118.26
8
ATOM 6362 N PRO C 364 67.511 108.735-21.861 1.00137.80
7
ATOM 6363 CD PRO C 364 68.608 109.457-22.525 1.00159.94
6
ATOM 6364 CA PRO C 364 66.316 109.575-21.780 1.00137.10
6
ATOM 6365 CB PRO C 364 66.791 110.902-22.367 1.00160.15
6
ATOM 6366 CG PRO C 364 67.859 110.479-23.332 1.00160.69
6
ATOM 6367 C PRO C 364 65.081 109.033-22.499 1.00136.05
6
ATOM 6368 0 PRO C 364 63.962 109.211-22.013 1.00136.06
8
ATOM 6369 N ASP C 365 65.275 108.375-23.643 1.00 91.02
7
ATOM 6370 CA ASP C 365 64.146 107.837-24.415 1.00 89.06
6
ATOM 6371 CB ASP C 365 64.462 107.865-25.918 1.00162.10
6
ATOM 6372 CG ASP C 365 63.205 107.890-26.783 1.00163.67
6
ATOM 6373 OD1 ASP C 365 62.364 106.980-26.647 1.00165.13
8
ATOM 6374 OD2 ASP C 365 63.058 108.823-27.601 1.0'0164.24
8
ATOM 6375 C ASP C 365 63.768 106.416-23.994 1.00 87.01
6
114

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ATOM 6376 0 ASP C 365 62.605 106.016-24.084 1.00 86.49
8
ATOM 6377 N THR C 366 64.753 105.655-23.535 1.00177.30
7
ATOM 6378 CA THR C 366 64.495 104.298-23.090 1.00175.40
6
ATOM 6379 CB THR C 366 65.800 103.518-22.886 1.00130.83
6
ATOM 6380 OG1 THR C 366 66.573 103.560-24.091 1.00131.14
8
ATOM 6381 CG2 THR C 366 65.504 102.069-22.527 1.00131.24
6
ATOM 6382 C THR C 366 63.766 104.385-21.759 1.00173.92
6
ATOM 6383 0 THR C 366 63.205 103.402-21.282 1.00175.00
8
ATOM 6384 N LEU C 367 63.773 105.578-21.170 1.00 79.11
7
ATOM 6385 CA LEU C 367 63.127 105.812-19.884 1.00 75.66
6
ATOM 6386 CB LEU C 367 63.909 106.844-19.080 1.00 69.38
6
ATOM 6387 CG LEU C 367 65.295 106.413-18.623 1.00 68.94
6
ATOM 6388 CD1 LEU C 367 65.850 107.463-17.690 1.00 68.92
6
ATOM 6389 CD2 LEU C 367 65.214 105.068-17.918 1.00 69.19
6
ATOM 6390 C LEU C 367 61.692 106.281-19.989 1.00 73.59
6
ATOM 6391 0 LEU C 367 61.388 107.221-20.713 1.00 73.53
8
ATOM 6392 N THR C 368 60.813 105.620-19.249 1.00 96.65
7
ATOM 6393 CA THR C 368 59.402 105.973-19.224 1.00 94.95
6
ATOM 6394 CB THR C 368 58.624 105.319-20.382 1.00107.98
6
ATOM 6395 OG1 THR C 368 58.611 103.899-20.207 1.60107.29
8
ATOM 6396 CG2 THR C 368 59.261 105.655-21.717 1.00108.58
6
ATOM 6397 C THR C 368 58.837 105.450-17.910 1.00 93.31
6
ATOM 6398 0 THR C 368 59.460 104.619-17.245 1.00 94.32
8
ATOM 6399 N PRO C 369 57.655 105.935-17.508 1.00 69.12
7
ATOM 6400 CD PRO C 369 56.695 106.779-18.229 1.00 28.46
6
ATOM 6401 CA PRO C 369 57.075 105.451-16.255 1.00 67.49
6
ATOM 6402 CB PRO C 369 55.753 106.202-16.178 1.00 28.00
6
ATOM 6403 CG PRO C 369 55.401 106.382-17.583 1.00 27.23
6
ATOM 6404 C PRO C 369 56.882 103.953-16.385 1.00 66.12
6
ATOM 6405 0 PRO C 369 57.017 103.401-17.476 1.00 66.14
8
ATOM 6406 N ALA C 370 56.564 103.292-15.283 1.00 93.49
7
ATOM 6407 CA ALA C 370 56.382 101.851-15.314 1.00 91.62
6
ATOM 6408 CB ALA C 370 55.515 101.446-16.497 1.00 18.84
6
ATOM 6409 C ALA C 370 57.754 101.223-15.444 1.00 90.43
6
ATOM 6410 0 ALA C 370 57.928 100.042-15.154 1.00 89.81
8
ATOM 6411 N LYS C 371 58.729 102.023-15.874 1.00 47.06
7
ATOM 6412 CA LYS C 371 60.089 101.532-16.046 1.00 46.49
6
ATOM 6413 CB LYS C 371 60.581 101.857-17.458 1.00103.72
6
ATOM 6414 CG LYS C 371 59.697 101.217-18.521 1.00104.63
6
ATOM 6415 CD LYS C 371 60.192 101.434-19.935 1.00104.61
6
ATOM 6416 CE LYS C 371 59.220 100.810-20.932 1.00105.32
6
ATOM 6417 NZ LYS C 371 57.824 101.341-20.797 1.00103.82
7
ATOM 6418 C LYS C 371 61.057 102.057-14.995 1.00 45.54
6
ATOM 6419 0 LYS C 371 61.917 101.306-14.512 1.00 44.46
8
ATOM 6420 N LEU C 372 60.909 103.338-14.645 1.00 69.08
7
ATOM 6421 CA LEU C 372 61.749 103.989-13.624 1.00 68.75
6
ATOM 6422 CB LEU C 372 61.683 105.522-13.739 1.00 76.05
6
ATOM 6423 CG LEU C 372 62.355 106.304-14.874 1.00 75.32
6
ATOM 6424 CD1 LEU C 372 61.856 107.738-14.834 1.00 74.70
6
ATOM 6425 CD2 LEU C 372 63.874 106.257-14.740 1.00 76.45
6
ATOM 6426 C LEU C 372 61.180 103.592-12.274 1.00 67.85
6
ATOM 6427 0 LEU C 372 61.875 103.610-11.253 1.00 66.75
8
ATOM 6428 N VAL C 373 59.894 103.248-12.307 1.00 58.10
7
ATOM 6429 CA VAL C 373 59.124 102.836-11.146 1.00 57.39
6
ATOM 6430 CB VAL C 373 57.690 103.234-11.324 1.00 57.10
6
ATOM 6431 CG1 VAL C 373 56.922 102.957-10.059 1.00 57.34
6
115

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ATOM 6432 CG2 VALC 373 57.621 104.691-11.740 1.00 56.99
6
ATOM 6433 C VALC 373 59.174 101.329-11.000 1.00 58.00
6
ATOM 6434 O VALC 373 59.004 100.599-11.978 1.00 58.26
8
ATOM 6435 N ASNC 374 59.390 100.862-9.774 1.00 33.15
7
ATOM 6436 CA ASNC 374 59.493 99.425 -9.516 1.00 34.29
6
ATOM 6437 CB ASNC 374 60.964 99.025 -9.321 1.00128.77
6
ATOM 6438 CG ASNC 374 61.844 99.414 -10.501 1.00130.53
6
ATOM 6439 OD1 ASNC 374 61.560 99.063 -11.648 1.00132.45
8
ATOM 6440 ND2 ASNC 374 62.927 100.132-10.219 1.00130.55
7
ATOM 6441 C ASNC 374 58.695 98.997 -8.295 1.00 34.29
6
ATOM 6442 0 ASNC 374 59.224 98.306 -7.424 1.00 33.45
8
ATOM 6443 N SERC 375 57.427 99.416 -8.266 1.00 44.69
7
ATOM 6444 CA SERC 375 56.444 99.141 -7.210 1.00 46.27
6
ATOM 6445 CB SERC 375 55.100 98.822 -7.863 1.00208.87
6
ATOM 6446 OG SERC 375 54.709 99.864 -8.744 1.00208.87
8
ATOM 6447 C SERC 375 56.778 98.049 -6.196 1.00 46.12
6
ATOM 6448 0 SERC 375 56.040 97.066 -6.062 1.00 45.87
8
ATOM 6449 N ARGC 376 57.879 98.255 -5.475 1.00 32.74
7
ATOM 6450 CA ARGC 376 58.375 97.334 -4.458 1.00 32.98
6
ATOM 6451 CB ARGC 376 59.766 96.821 -4.840 1.00142.46
6
ATOM 6452 CG ARGC 376 59.794 95.829 -5.999 1.00146.04
6
ATOM 6453 CD ARGC 376 59.085 94.530 -5.637 1.00147.74
6
ATOM 6454 NE ARGC 376 59.292 93.491 -6.642 1.00149.33
7
ATOM 6455 CZ ARGC 376 58.718 92.292 -6.604 1.00149.85
6
ATOM 6456 NH1 ARGC 376 57.895 91.977 -5.612 1.00150.47
7
ATOM 6457 NH2 ARGC 376 58.967 91.406 -7.558 1.00149.73
7
ATOM 6458 C ARGC 376 58.453 98.018 -3.101 1.00 32.39
6
ATOM 6459 O ARGC 376 58.211 97.395 -2.076 1.00 31.97
8
ATOM 6460 N PROC 377 58.804 99.314 -3.074 1.00 46.43
7
ATOM 6461 CD PROC 377 59.159 100.179-4.213 1.00162.38
~6
ATOM 6462 CA PROC 377 58.906 100.066-1.819 1.00 45.85
6
ATOM 6463 CB PROC 377 59.516 101.392-2.261 1.00161.93
6
ATOM 6464 CG PROC 377 58.963 101.562-3.635 1.00162.91
6
ATOM 6465 C PROC 377 57.576 100.259-1.103 1.00 44.99
6
ATOM 6466 0 PROC 377 57.491 100.0430.101 1.00 44.13
8
ATOM 6467 N LEUC 378 56.544 100.675-1.830 1.00 64.77
7
ATOM 6468 CA LEUC 378 55.242 100.876-1.203 1.00 64.97
6
ATOM 6469 CB LEUC 378 54.240 101.449-2.199 1.00 83.43
6
ATOM 6470 CG LEUC 378 54.400 102.963-2.337 1.00 84.69
6
ATOM 6471 CD1 LEUC 378 55.849 103.294-2.676 1.00 85.16
6
ATOM 6472 CD2 LEUC 378 53.455 103.489-3.402 1.00 84.91
6
ATOM 6473 C LEUC 378 54.722 99.581 -0.605 1.00 64.76
6
ATOM 6474 0 LEUC 378 53.809 99.588 0.214 1.00 65.34
8
ATOM 6475 N GLUC 379 55.296 98.466 -1.038 1.00 42.46
7
ATOM 6476 CA GLUC 379 54.943 97.166 -0.497 1.00 41.70
6
ATOM 6477 CB GLUC 379 54.995 96.091 -1.580 1.00100.82
6
ATOM 6478 CG GLUC 379 54.363 94.776 -1.173 1.00103.75
6
ATOM 6479 CD GLUC 379 52.895 94.920 -0.812 1.00105.94
6
ATOM 6480 OE1 GLUC 379 52.151 95.538 -1.601 1.00107.50
8
ATOM 6481 OE2 GLUC 379 52.481 94.409 0.254 1.00107.21
8
ATOM 6482 C GLUC 379 56.117 97.033 0.457 1.00 40.89
6
ATOM 6483 0 GLUC 379 57.064 97.811 ' 0.365 1.00 40.81
8
ATOM 6484 N ALAC 380 56.085 96.072 1.365 1.00100.07
7
ATOM 6485 CA ALAC 380 57.179 95.951 2.319 1.00 98.75
6
ATOM 6486 CB ALAC 380 58.546 96.036 1.603 1.00 32.13
6
ATOM 6487 C ALAC 380 57.030 97.111 3.299 1.00 97.64
6
116

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ATOM 6488 O ALAC 380 56.416 96.973 4.352 1.00 97.70
8
ATOM 6489 N ALAC 381 57.568 98.267 2.929 1.00 64.15
7
ATOM 6490 CA ALAC 381 57.505 99.443 3.788 1.00 63.00
6
ATOM 6491 CB ALAC 381 58.055 100.662 3.053 1.00 95.29
6
ATOM 6492 C ALAC 381 56.089 99.711 4.259 1.00 62.40
6
ATOM 6493 0 ALAC 381 55.882 100.389 5.260 1.00 62.60
8
ATOM 6494 N LEUC 382 55.110 99.174 3.543 1.00 43.72
7
ATOM 6495 CA LEUC 382 53.730 99.385 3.939 1.00 43.03
6
ATOM 6496 CB LEUC 382 52.910 99.874 2.755 1.00 18.57
6
ATOM 6497 CG LEUC 382 51.678 100.682 3.149 1.00 17.35
6
ATOM 6498 CD1 LEUC 382 52.011 101.661 4.281 1.00 16.89
6
ATOM 6499 CD2 LEUC 382 51.174 101.432 1.931 1.00 18.60
6
ATOM 6500 C LEUC 382 53.094 98.137 4.533 1.00 44.12
6
ATOM 6501 0 LEUC 382 52.279 98.223 5.453 1.00 43.72
8
ATOM 6502 N ARG_ 383 53.457 96.972 4.013 1.00111.91
C 7
ATOM 6503 CA ARGC 383 52.903 95.743 4.554 1.00113.91
6
ATOM 6504 CB ARGC 383 53.065 94.581 3.566 1.00196.78
6
ATOM 6505 CG ARGC 383 52.286 93.329 3.965 1.00200.48
6
ATOM 6506 CD ARGC 383 52.355 92.239 2.902 1.00203.04
6
ATOM 6507 NE ARGC 383 51.615 91.043 3.305 1.00203.93
7
ATOM 6508 CZ ARGC 383 51.507 89.943 2.565 1.00204.05
6
ATOM 6509 NH'1ARGC 383 52.091 89.879 1.374 1.00205.14
7
ATOM 6510 NH2 ARGC 383 50.816 88.904 3.015 1.00203.29
7
ATOM 6511 C ARGC 383 53.667 95.462 5.840 1.00113.54
6
ATOM 6512 0 ARGC 383 53.136 94.859 6.774 1.00114.76
8
ATOM 6513 N GLUC 384 54.914 95.924 5.882 1.00 34.91
7
ATOM 6514 CA GLUC 384 55.758 95.738 7.052 1.00 32:88
6
ATOM 6515 CB GLUC 384 57.159 96.280 6.797 1.00 64.39
6
ATOM 6516 CG GLUC 384 58.111 96.040 7.938 1.00 65.02
6
ATOM 6517 CD GLUC 384 59.470 95.580 7.467 1.00 65.71
6
ATOM 6518 OE1 GLUC 384 60.072 96.266 6.612 1.00 66.11
8
ATOM 6519 OE2 GLUC 384 59.936 94.529 7.956 1.00 66.58
8
ATOM 6520 C GLUC 384 55.133 96.458 8.230 1.00 31.68
6
ATOM 6521 0 GLUC 384 55.093 95.928 9.337 1.00 32.24
8
ATOM 6522 N PHEC 385 54.636 97.669 7.996 1.00 42.85
7
ATOM 6523 CA PHEC 385 53.993 98.427 9.067 1.00 42.02
6
ATOM 6524 CB PHEC 385 53.570 99.817 8.589 1.00 39.62
6
ATOM 6525 CG PHEC 385 52.555 100.480 9.484 1.00 39.09
6
ATOM 6526 CD1 PHEC 385 52.914 100.936 10.746 1.00 38.59
6
ATOM 6527 CD2 PHEC 385 51.236 100.607 9.082 1.00 39.56
6
ATOM 6528 CE1 PHEC 385 51.977 101.504 11.594 1.00 37.75
6
ATOM 6529 CE2 PHEC 385 50.293 101.171 9.922 1.00 39.49
6
ATOM 6530 CZ PHEC 385 50.662 101.620 11.179 1.00 38.51
6
ATOM 6531 C PHEC 385 52.756 97.691 9.555 1.00 40.74
6
ATOM 6532 0 PHEC 385 52.773 97.085 10.621 1.00 40.68
8
ATOM 6533 N PHEC 386 51.688 97.740 8.765 1.00 28.00
7
ATOM 6534 CA PHEC 386 50.450 97.090 9.142 1.00 27.86
6
ATOM 6535 CB PHEC 386 49.583 96.816 7.916 1.00 48.14
6
ATOM 6536 CG PHEC 386 48.604 97.905 7.641 1.00 47.19
6
ATOM 6537 CD1 PHEC 386 48.912 98.915 6.739 1.00 46.13
6
ATOM 6538 CD2 PHEC 386 47.418 97.989 8.375 1.00 46.56
6
ATOM 6539 CE1 PHEC 386 48.060 100.004 6.570 1.00 44.96
6
ATOM 6540 CE2 PHEC 386 46.557 99.075 8.217 1.00 44.71
6
ATOM 6541 CZ PHEC 386 46.880 100.085 7.314 1.00 44.87
6
ATOM 6542 C PHEC 386 50.594 95.824 9.966 1.00 28.13
6
ATOM 6543 0 PHEC 386 49.966 95.712 11.009 1.00 27.05
8
117

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ATOM 6544 N SERC 387 51.401 94.862 9.533 1.00 26.30
ATOM 6545 CA SERC 387 51.539 93.658 10.342 1.00 26.99
ATOM 6546 CB SERC 387 51.007 92.444 9.588 1.00123.05
ATOM 6547 OG SERC 387 51.207 91.255 10.333 1.00126.81
ATOM 6548 C SERC 387 52.967 93.399 10.753 1.00 26.19
ATOM 6549 0 SERC 387 53.561 92.448 10.290 1.00 25.05
ATOM 6550 N ARGC 388 53.502 94.244 11.625 1.00 37.99
ATOM 6551 CA ARGC 388 54.870 94.131 12.114 1.00 38.08
ATOM 6552 CB ARGC 388 55.883 94.089 10.960 1.00112.87
ATOM 6553 CG ARGC 388 56.209 92.692 10.435 1.00116.98
ATOM 6554 CD ARGC 388 56.968 92.739 9.115 1.00119.48
ATOM 6555 NE ARGC 388 57.168 91.404 8.555 1.00121.55
ATOM 6556 CZ ARGC 388 57.689 91.169 7.355 1.00122.29
ATOM 6557 NH1 ARGC 388 58.063 92.179 6.585 2.00121.93
ATOM 6558 NH2 ARGC 388 57.838 89.925 6.925 1.00123.26
ATOM 6559 C ARGC 388 55.160 95.331 12.992 1.00 35.86
ATOM 6560 0 ARGC 388 56.278 95.533 13.454 1.00 36.12
ATOM 6561 N SERC 389 54.144 96.150 13.197 1.00 45.39
ATOM 6562 CA SERC 389 54.284 97.320 14.046 1.00 43.00
ATOM 6563 CB SERC 389 53.325 98.432 13.611 1.00 39.12
ATOM 6564 OG SERC 389 53.341 99.510 14.528 1.00 35.94
ATOM 6565 C SERC 389 53.889 96.839 15.419 1.00 42.79
ATOM 6566 0 SERC 389 53.447 95.696 15.573 1.00 42.20
ATOM 6567 N GLNC 390 54.084 97.693 16.419 1.00 43.33
ATOM 6568 CA GLNC 390 53.683 97.352 17.770 1.00 41.60
ATOM 6569 CB GLNC 390 54.782 97.640 18.811 1.00 54.20
ATOM 6570 CG GLNC 390 55.759 98.776 18.515 1.00 55.42
ATOM 6571 CD GLNC 390 56.733 99.036 19.681 1.00 57.75
ATOM 6572 OE1 GLNC 390 57.351 98.112 20.216 1.00 57.83
ATOM 6573 NE2 GLNC 390 56.871 100.304 20.066 1.00 59.66
ATOM 6574 C GLNC 390 52.462 98.195 18.012'1.00 39.24
ATOM 6575 0 GLNC 390 51.910 98.210 19.096 1.00 39.78
ATOM 6576 N LEUC 391 52.048 98.904 16.973 1.00 22.08
ATOM 6577 CA LEUC 391 50.861 99.723 17.069 1.00 21.29
ATOM 6578 CB LEUC 391 51.148 101.173 16.652 1.00 15.77
~
ATOM 6579 CG LEUC 391 50.698 102.369 17.508 1.00 13.87
ATOM 6580 CD1 LEUC 391 51.031 102.92 18.960 1.00 13.87
ATOM 6581 CD2 LEUC 391 51.411 103.590 17.014 1.00 13.87
ATOM 6582 C LEUC 391 49.820 99.089 16.193 1.00 22.68
ATOM 6583 0 LEUC 391 48.774 99.678 15.942 1.00 22.06
ATOM 6584 N SERC 392 50.139 97.909 15.671 1.00 43.95
ATOM 6585 CA SERC 392 49.164 97.169 14.890 1.00 46.21
ATOM 6586 CB SERC 392 49.715 96.792 13.557 1.00 85.53
ATOM 6587 OG SERC 392 48.836 95.907 12.903 1.00 90.30
ATOM 6588 C SERC 392 48.967 95.890 15.691 1.00 46.84
ATOM 6589 O SERC 392 49.482 94.831 15.306 1.00 45.92
ATOM 6590 N GLNC 393 48.147 95.990 16.740 1.00 47.13
ATOM 6591 CA GLNC 393 47.832 94.925 17.667 1.00 47.20
ATOM 6592 CB GLNC 393 47.217 95.540 18.898 1.00 30.87
ATOM 6593 CG GLNC 393 47.925 96.720 19.395 1.00 28.44
ATOM 6594 CD GLNC 393 47.360 97.211 20.675 1.00 25.75
ATOM 6595 OE1 GLNC 393 46.354 96.725 21.166 1.00 24.51
ATOM 6596 NE2 GLNC 393 48.006 98.187 21.235 1.00 23.12
ATOM 6597 C GLNC 393 46.929 93.850 17.118 1.00 47.76
ATOM 6598 O GLNC 393 46.520 93.884 15.954 1.00 47.04
ATOM 6599 N PHEC 394 46.632 92.891 17.987 1.00100.33
118

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ATOM 6600 CA PHE C 394 45.819 91.735 17.611 1.00101.57
ATOM 6601 CB PHE C 394 46.088 90.558 18.496 1.00 55.89
ATOM 6602 CG PHE C 394 45.937 89.199 17.814 1.00 56.43
ATOM 6603 CD1 PHE C 394 .47.022 88.610 17.163 1.00 56.75
ATOM 6604 CD2 PHE C 394 44.729 88.501 17.847 1.00 56.42
ATOM 6605 CE1 PHE C 394 46.903 87.378 16.571 1.00 55.89
ATOM 6606 CE2 PHE C 394 44.614 87.263 17.251 1.00 56.09
ATOM 6607 CZ PHE C 394 45.701 86.706 16.613 1.00 56.21
ATOM 6608 C PHE C 394 44.386 92.136 17.908 1.00102.12
ATOM 6609 0 PHE C 394 44.148 93.111 18.590 1.00105.06
ATOM 6610 N LYS C 395 43.398 91.433 17.396 1.00 43.81
ATOM 6611 CA LYS C 395 42.047 91.882 17.648 1.00 42.16
ATOM 6612 CB LYS C 395 41.163 91.329 16.587 1.00 29.19
ATOM 6613 CG LYS C 395 39.781 91.752 16.820 1.00 28.08
ATOM 6614 CD LYS C 395 39.421 92.968 15.985 1.00 27.79
ATOM 6615 CE LYS C 395 37.907 93.165 15.857 1.00 28.90
ATOM 6616 NZ LYS C 395 37.246 92.603 14.623 1.00 30.18
ATOM 6617 C LYS C 395 41.661 91.354 18.981 1.00 42.33
ATOM 6618. 0 LYS C 395 41.706 90.197 19.193 1.00 44.26
ATOM 6619 N ASP C 396 41.363 92.120 19.918 1.00 50.17
ATOM 6620 CA ASP C 396 40.920 91.730 21.255 1.00 49.83
ATOM 6621 CB ASP C 396 40.968 92.942 22.177 1.00 62.41
ATOM 6622 CG ASP C 396 40.953 92.600 23.650 1.00 63.51
ATOM 6623 OD1 ASP C 396 39.859 92.509 24.213 1.00 64.56
ATOM 6624 OD2 ASP C 396 42.030 92.530 24.284 1.00 62.94
ATOM 6625 C ASP C 396 39.518 91.320 20.926 1.00 49.88
ATOM 6626 0 ASP C 396 38.765 92.120 20.429 1.00 50.51
ATOM 6627 N GLU C 397 39.080 90.123 21.021 1.00 56.43
ATOM 6628 CA GLU C 397 37.715 89.814 20.676 1.00 55.07
ATOM 6629 CB GLU C 397 37.666 89.323 19.251 1.00 49.1'7
ATOM 6630 CG GLU C 397 37.220 90.347 18.256 1.00 54.30
ATOM 6631 CD GLU C 397 37.118 89.754 16.863 1.00 58.33
ATOM 6632 OE1 GLU C 397 36.404 90.376 16.030 1.00 60.41
ATOM 6633 OE2 GLU C 397 37.749 88.677 16.650 1.00 60.82
ATOM 6634 C GLU C 397 37.006 88.814 21.516 1.00 52.66
ATOM 6635 O GLU C 397 36.171 88.083 20.991 1.00 53.22
ATOM 6636 N THR C 398 37.320 88.765 22.800 1.00 28.42
ATOM 6637 CA THR C 398 36.665 87.811 23.652 1.00 25.27
ATOM 6638 CB THR C 398 37.247 87.863 25.038 1.00 19.82
ATOM 6639 OG1 THR C 398 38.607 88.268 24.933 1.00 19.57
ATOM 6640 CG2 THR C 398 37.222 86.489 25.695 1.00 18.63
ATOM 6641 C THR C 398 35.177 88.132 23.682 1.00 25.57
ATOM 6642 O THR C 398 34.360 87.249 23.430 1.00 26.94
ATOM 6643 N ASN C 399 34.811 89.379 23.988 1.00 37.48
ATOM 6644 CA ASN C 399 33.392 89.759 23.996 1.00 35.58
ATOM 6645 CB ASN C 399 32.902 90.052 25.448 1.00 21.35
ATOM 6646 CG ASN C 399 32.983 91.536 25.855 1.00 19.01
ATOM 6647 OD1 ASN C 399 32.951 91.854 27.043 1.00 17.99
ATOM 6648 ND2 ASN C 399 33.059 92.437 24.884 1.00 17.39
ATOM 6649 C ASN C 399 33.212 90.945 23.040 1.00 35.20
ATOM 6650 O ASN C 399 34.146 91.715 22.825 1.00 35.32
ATOM 6651 N PRO C 400 32.015 91.103 22.442 1.00 48.40
ATOM 6652 CD PRO C 400 30.737 90.377 22.567 1.00 16.55
ATOM 6653 CA PRO C 400 31.877 92.238 21.531 1.00 47.22
ATOM 6654 CB PRO C 400 30.374 92.226 21.168 1.00 14.25
ATOM 6655 CG PRO C 400 29.724 91.456 22.247 1.00 13.87
119

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ATOM 6656 C PROC 400 32.393 93.590 22.040 1.00 46.64 6
ATOM 6657 O PROC 400 33.057 94.295 21.290 1.00 46.70 8
ATOM 6658 N LEUC 401 32.126 93.962 23.290 1.00 28.24 7
ATOM 6659 CA LEUC 401 32.618 95.259 23.764 1.00 27.85 6
ATOM 6660 CB LEUC 401 32.372 95.455 25.262 1.00 13.87 6
ATOM 6661 CG LEUC 401 33.105 96.654 25.889 1.00 13.87 6
ATOM 6662 CD1 LEUC 401 32.619 97.953 25.329 1.00 13.87 6
ATOM 6663 CD2 LEUC 401 32.886 96.633 27.359 1.00 13.87 6
ATOM 6664 C LEUC 401 34.109 95.417 23.486 1.00 28.34 6
ATOM 6665 0 LEUC 401 34.541 96.405 22.876 1.00 28.00 8
ATOM 6666 N SERC 402 34.879 94.434 23.946 1.00 13.95 7
ATOM 6667 CA SERC 402 36.327 94.383 23.779 1.00 15.21 6
ATOM 6668 CB SERC 402 36.784 92.924 23.812 1.00 38.02 6
ATOM 6669 OG SERC 402 36.100 92.173 24.805 1.00 39.19 8
ATOM 6670 C SERC 402 36.778 95.020 22.465 1.00 16.19 6
ATOM 6671 0 SERC 402 37.133 96.182 22.439 1.00 16.43 8
ATOM 6672 N SERC 403 36.760 94.251 21.380 1.00 15.62 7
ATOM 6673 CA SERC 403 37.176 94.747 20.079 1.00 17.34 6
ATOM 6674 CB SERC 403 36.604 93.877 18.963 1.00 62.46 6
ATOM 6675 OG SERC 403 35.382 94.419 18.498 1.00 67.82 8
ATOM 6676 C SERC 403 36.755 96.199 19.860 1.00 17.00 6
ATOM 6677 0 SERC 403 37.539 97.002 19.346 1.00 15.83 8
ATOM 6678 N LEUC 404 35.521 96.540 20.234 1.00 27.97 7
ATOM 6679 CA LEUC 404 35.026 97.916 20.092 1.00 28.89 6
ATOM 6680 CB LEUC 404 33.603 98.034 20.672 1.00 19.74 6
ATOM 6681 CG LEUC 404 32.946 99.424 20.794 1.00 19.85 6
ATOM 6682 CD1 LEUC 404 33.091 100.242 19.523 1.00 19.19 6
ATOM 6683 CD2 LEUC 404 31.480 99.249 21.115 1.00 19.89 6
ATOM 6684 C LEUC 404 36.000 98.822 20.852 1.00 29.54 6
ATOM 6685 0 LEUC 404 36.624 99.719 20.285 1.00 28.10 8
ATOM 6686 N ARGC 405 36.136 98.552 22.141 1.00 35.76 7
ATOM 6687 CA ARGC 405 37.039 99.292 23.000 1.00 38.91 6
ATOM 6688 CB ARGC 405 37.145 98.572 24.342 1.00 66.63 6
ATOM 6689 CG ARGC 405 38.233 99.090 25.252 1.00 71.12 6
ATOM 6690 CD ARGC 405 38.340 98.252 26.518 1.00 73.97 6
ATOM 6691 NE ARGC 405 39.437 98.713 27.360 1.00 76.85 7
ATOM 6692 CZ ARGC 405 39.900 98.054 28.416 1.00 78.23 6
ATOM 6693 NH1 ARGC 405 39.362 96.894 28.771 1.00 78.51 7
ATOM 6694 NH2 ARGC 405 40.913 98.555 29.111 1.00 77.42 7
ATOM 6695 C ARGC 405 38.427 99.411 22.381 1.00 40.01 6
ATOM 6696 0 ARGC 405 39.073 100.452 22.447 1.00 40.60 8
ATOM 6697 N HISC 406 38.886 98.338 21.761 1.00 71.68 7
ATOM 6698 CA HISC 406 40.214 98.343 21.181 1.00 71.27 6
ATOM 6699 CB HISC 406 40.476 97.032 20.471 1.00 45.63 6
ATOM 6700 CG HISC 406 41.925 96.763 20.246 1.00 45.60 6
ATOM 6701 CD2 HISC 406 42.673 95.666 20.502 1.00 45.37 6
ATOM 6702 ND1 HISC 406 42.777 97.691 19.694 1.00 45.64 7
ATOM 6703 CE1 HISC 406 43.990 97.177 19.620 1.00 46.13 6
ATOM 6704 NE2 HISC 406 43.954 95.949 20.104 1.00 45.14 7
ATOM 6705 C HISC 406 40.507 99.480 20.220 1.00 70.89 6
ATOM 6706 0 HISC 406 41.665 99.834 20.013 1.00 71.72 8
ATOM 6707 N LYSC 407 39.474 100.052 19.621 1.00 32.66 7
ATOM 6708 CA LYSC 407 39.699 101.145 18.682 1.00 32.24 6
ATOM 6709 CB LYSC 407 38.658 101.092 17.572 1.00 49.47 6
ATOM 6710 CG LYSC 407 37.803 99.846 17.643 1.00 50.89 6
ATOM 6711 CD LYSC 407 37.825 99.073 16.360 1.00 51.05 6
120

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ATOM 6712 CE LYS C407 36.856 97.930 16.448 1.00 50.82
6
ATOM 6713 NZ LYS C407 36.828 97.249 15.154 1.00 54.18
7
ATOM 6714 C LYS C407 39.613 102.453 19.435 1.00 31.38
6
ATOM 6715 O LYS C407 40.315 103.415 19.135 1.00 30.71
8
ATOM 6716 N ARG C408 38.742 102.463 20.430 1.00 37.35
7
ATOM 6717 CA ARG C408 38.541 103.623 21.267 1.00 37.07
6
ATOM 6718 CB ARG C408 37.306 103.398 22.149 1.00 46.72
6
ATOM 6719 CG ARG C408 36.014 103.190 21.383 1.00 46.51
6
ATOM 6720 CD ARG C408 35.442 104.515 20.974 1.00 48.61
6
ATOM 6721 NE ARG C408 35.078 105.309 22.145 1.00 50.98
7
ATOM 6722 CZ ARG C408 34.973 106.638 22.156 1.00 52.08
6
ATOM 6723 NH1 ARG C408 35.204 107.354 21.058 1.00 52.11
7
ATOM 6724 NH2 ARG C408 34.643 107.259 23.273 1.00 51.65
7
ATOM 6725 C ARG C408 39.796 103.807 22.132 1.00 36.51
6
ATOM 6726 0 ARG C408 39.803 104.612 23.066 1.00 36.16
8
ATOM 6727 N ARG C409 40.862 103.070 21.813 1.00 35.92
7
ATOM 6728 CA ARG C409 42.100 103.162 22.593 1.00 35.68
6
ATOM 6729 CB ARG C409 42.737 101.794 22.782 1.00 37.02
6
ATOM 6730 CG ARG C409 44.122 101.843 23.419 1.00 35.78
6
ATOM 6731 CD ARG C409 44.655 100.438 23.564 1.00 36.76
6
ATOM 6732 NE ARG C409 43.710 99.638 24.333 1.00 38.64
7
ATOM 6733 CZ ARG C409 43.783 98.323 24.497 1.00 39.22
6
ATOM 6734 NH1 ARG C409 44.767 97.629 23.948 1.00 39.62
7
ATOM 6735 NH2 ARG C409 42.857 97.701 25.208 1.00 41.22
7
ATOM 6736 C ARG C409 43.178 104.088 22.086 1.00 35.88
6
ATOM 6737 0 ARG C409 43.510 104.107 20.896 1.00 35.91
8
ATOM 6738 N ILE C410 43.729 104.842 23.030 1.00 35.02
7
ATOM 6739 CA ILE C410 44.799 105.785 22.765 1.00 36.04
6
ATOM 6740 CB ILE C410 44.591 107.153 23.528 1.00 32.78
6
ATOM 6741 CG2 ILE C410 45.939 107.795 23.826 1.00 34.25
6
ATOM 6742 CG1 ILE C410 43.759 108.137 22.696 1.00 31.74
6
ATOM 6743 CD1 ILE C410 42.278 107.862 22.649 1.00 30.33
6
ATOM 6744 C ILE C410 46.034 105.089 23.318 1.00 37.94
6
ATOM 6745 0 ILE C410 46.056 104.717 24.493 1.00 38.10
8
ATOM 6746 N SER C411 47.038 104.885 22.471 1.00 24.18
7
ATOM 6747 CA SER C411 48.268 104.250 22.909 1.00 27.82
6
ATOM 6748 CB SER C411 48.783 103.266 21.861 1.00157.88
6
ATOM 6749 OG SER C411 48.934 101.957 22.435 1.00162.92
8
ATOM 6750 C SER C411 49.328 105.306 23.163 1.00 28.22
6
ATOM 6751 O SER C411 49.157 106.472 22.810 1.00 27.60
8
ATOM 6752 N ALA C412 50.424 104.904 23.783 1.00 55.73
7
ATOM 6753 CA ALA C412 51.484 105.844 24.063 1.00 58.06
6
ATOM 6754 CB ALA C412 51.304 106.428 25.444 1.00 70.88
6
ATOM 6755 C ALA C412 52.792 105.094 23.969 1.00 59.54
6
ATOM 6756 0 ALA C412 53.835 105.674 23.677 1.00 61.33
8
ATOM 6757 N LEU C413 52.763 103.845 23.911 1.00 56.28
7
ATOM 6758 CA LEU C413 53.917 102.970 24.143 1.00 56.99
6
ATOM 6759 CB LEU C413 53.486 101.686 24.859 1.00 33.97
6
ATOM 6760 CG LEU C413 53.462 101.732 26.389 1.00 33.75
6
ATOM 6761 CD1 LEU C413 53.010 100.396 26.960 1.00 34.03
6
ATOM 6762 CD2 LEU C413 54.829 102.111 26.937 1.00 33.01
6
ATOM 6763 C LEU C413 54.633 102.619 22.836 1.00 58.26
6
ATOM 6764 0 LEU C413 55.800 102.271 22.908 1.00 57.86
8
ATOM 6765 N GLY C414 54.461 102.472 21.897 1.00 55.40
7
ATOM 6766 CA GLY C414 54.429 102.388 20.433 1.00 58.04
6
ATOM 6767 C GLY C414 55.480 103.322 19.847 1.00 60.19
6
121

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ATOM 6768 0 GLY C 414 55.907 104.24920.529 1.00 59.87
8
ATOM 6769 N PRO C 415 55.778 103.59218.679 1.00 67.48
7
ATOM 6770 CD PRO C 415 54.565 103.10017.851 1.00 42.19
6
ATOM 6771 CA PRO C 415 56.957 103.77617.822 1.00 69.41
6
ATOM 6772 CB PRO C 415 56.489 103.23816.456 1.00 43.88
6
ATOM 6773 CG PRO C 415 55.322 102.38216.781 1.00 42.95
6
ATOM 6774 C PRO C 415 57.383 105.24417.719 1.00 71.76
6
ATOM 6775 O PRO C 415 58.644 105.42517.279 1.00 72.81
8
ATOM 6776 N GLY C 416 57.069 106.15017.236 1.00 73.40
7
ATOM 6777 CA GLY C 416 56.907 107.59517.251 1.00 76.08
6
ATOM 6778 C GLY C 416 58.056 108.22718.015 1.00 78.75
6
ATOM 6779 O GLY C 416 58.600 107.57418.941 1.00 79.56
8
ATOM 6780 N GLY C 417 58.491 109.38717.553 1.00208.87
7
ATOM 6781 CA GLY C 417 59.641 110.07318.153 1.00208.87
6
ATOM 6782 C GLY C 417 59.492 110.30919.660 1.00208.87
6
ATOM 6783 0 GLY C 417 58.525 110.97920.105 1.00208.87
8
ATOM 6784 N LEU C 418 60.613 109.82920.407 1.00178.48
7
ATOM 6785 CA LEU C 418 60.715 110.08621.845 1.00178.98
6
ATOM 6786 CB LEU C 418 60.914 111.58722.111 1.00115.94
6
ATOM 6787 CG LEU C 418 61.291 112.00423.540 1.00116.07
6
ATOM 6788 CD1 LEU C 418 62.642 111.40623.903 1.00115.88
6
ATOM 6789 CD2 LEU C 418 61.336 113.52323.650 1.00115.50
6
ATOM 6790 C LEU C 418 59.450 109.57822.554 1.00179.32
6
ATOM 6791 O LEU C 418 59.110 110.01823.654 1.00179.90
8
ATOM 6792 N THR C 419 58.770 108.63721.901 1.00 96.26
7
ATOM 6793 CA THR C 419 57.544 108.02522.404 1.00 95.44
6
ATOM 6794 CB THR C 419 56.928 107.11721.340 1.00 56.35
6
ATOM 6795 OG1 THR C 419 56.537 107.90820.215 1.00 56.39
8
ATOM 6796 CG2 THR C 419 55.727 106.38321.889 1.00 56.02
6
ATOM 6797 C THR C 419 57.804 107.17623.635 1.00 96.03
6
ATOM 6798 0 THR C 419 58.207 106.02023.508 1.00 96.11
8
ATOM 6799 N ARG C 420 57.558 107.74524.814 1.00 77.71
7
ATOM 6800 CA ARG C 420 57.770 107.05526.086 1.00 77.96
6
ATOM 6801 CB ARG C 420 56.605 107.32627.046 1.00104.02
6
ATOM 6802 CG ARG C 420 55.915 108.67426.853 1.00105.07
6
ATOM 6803 CD ARG C 420 56.881 109.84327.005 1.00104.95
6
ATOM 6804 NE ARG C 420 56.216 111.13926.869 1.00105.26
7
ATOM 6805 CZ ARG C 420 56.834 112.31426.984 1.00106.46
6
ATOM 6806 NH1 ARG C 420 58.138 112.36627.234 1.00106.73
7
ATOM 6807 NH2 ARG C 420 56.146 113.44226.857 1.00106.90
7
ATOM 6808 C ARG C 420 57.879 105.55425.854 1.00 78.23
6
ATOM 6809 0 ARG C 420 56.919 104.92825.390 1.00 77.77
8
ATOM 6810 N GLU C 421 59.052 104.98126.127 1.00 66.20
7
ATOM 6811 CA GLU C 421 -59.214 103.54325.959 1.00 67.97
6
ATOM 6812 CB GLU C 421 60.691 103.15125.841 1.00129.07
6
ATOM 6813 CG GLU C 421 61.453 103.08127.157 1.00131.33
6
ATOM 6814 CD GLU C 421 62.674 102.17027.080 1.00133.12
6
ATOM 6815 OE1 GLU C 421 63.577 102.44226.260 1.00134.20
8
ATOM 6816 OE2 GLU C 421 62.731 101.17727.842 1.00134.22
8
ATOM 6817 C GLU C 421 58.609 102.96427.233 1.00 68.71
6
ATOM 6818 O GLU C 421 58.182 103.72428.104 1.00 68.46
8
ATOM 6819 N ARG C 422 58.562 101.64127.353 1.00 76.71
7
ATOM 6820 CA ARG C 422 57.993 101.02128.546 1.00 78.22
6
ATOM 6821 CB ARG C 422 58.513 99.586 28.707 1.00169.99
6
ATOM 6822 CG ARG C 422 60.001 99.471 29.013 1.00172.23
6
ATOM 6823 CD ARG C 422 60.409 98.015 29.221 1.00174.06
6
122

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ATOM 6824 NE ARG C 422 59.588 97.355 30.236 1.00175.15 7
ATOM 6825 CZ ARG C 422 59.774 96.108 30.662 1.00175.32 6
ATOM 6826 NH1 ARG C 422 60.759 95.373 30.166 1.00175.51 7
ATOM 6827 NH2 ARG C 422 58.973 95.594 31.586 1.00175.57 7
ATOM 6828 C ARG C 422 58.365 101.85329.766 1.00 78.41 6
ATOM 6829 O ARG C 422 57.577 101.99930.691 1.00 77.73 8
ATOM 6830 N ALA C 423 59.572 102.41129.742 1.00 83.10 7
ATOM 6831 CA ALA C 423 60.073 103.25330.823 1.00 83.57 6
ATOM 6832 CB ALA C 423 61.008 102.44231.717 1.00150.52 6
ATOM 6833 C ALA C 423 60.799 104.50730.293 1.00 83.97 6
ATOM 6834 0 ALA C 423 61.761 104.98230.903 1.00 83.60 8
ATOM 6835 N GLY C 424 60.318 105.04229.170 1.00195.59 7
ATOM 6836 CA GLY C 424 60.923 106.22528.569 1.00196.13 6
ATOM 6837 C GLY C 424 61.202 107.34329.559 1.00196.47 6
ATOM 6838 0 GLY C 424 60.606 107.37330.636 1.00196.41 8
ATOM 6839 N PHE C 425 62.092 108.27129.200 1.00134.14 7
ATOM 6840 CA PHE C 425 62.436 109.37030.102 1.00133.29 6
ATOM 6841 CB PHE C 425 63.622 110.18229.559 1.00106.82 6
ATOM 6842 CG PHE C 425 64.887 109.99630.360 1.00107.16 6
ATOM 6843 CD1 PHE C 425 65.712 108.89430.144 1.00106.92 6
ATOM 6844 CD2 PHE C 425 65.210 110.87731.393 1.00107.62 6
ATOM 6845 CE1 PHE C 425 66.838 108.66530.950 1.00107.04 6
ATOM 6846 CE2 PHE C 425 66.335 110.65632.205 1.00108.30 6
ATOM 6847 CZ PHE C 425 67.147 109.54631.981 1.00107.74 6
ATOM 6848 C PHE C 425 61.284 110.30730.458 1.00132.93 6
ATOM 6849 0 PHE C 425 61.031 111.29429.764 1.00133.62 8
ATOM 6850 N ASP C 426 60.618 109.96031.564 1.00158.69 7
ATOM 6851 CA ASP C 426 59.477 110.65432.177 1.00157.54 6
ATOM 6852 CB ASP C 426 59.177 112.01031.500 1.00 82.48 6
ATOM 6853 CG ASP C 426 58.398 111.87830.205 1.00 81.86 6
ATOM 6854 OD1 ASP C 426 58.453 110.80129.573 1.00 81.36 8
ATOM 6855 OD2 ASP C 426 57.740 112.87229.817 1.00 80.99 8
ATOM 6856 C ASP C 426 58.237 109.75732.193 1.00156.95 6
ATOM 6857 O ASP C 426 57.144 110.17531.827 1.00157.14 8
ATOM 6858 N VAL C 427 58.433 108.51532.631 1.00169.51 7
ATOM 6859 CA VAL C 427 57.359 107.52832.730 1.00168.75 6
ATOM 6860 CB VAL C 427 57,885 106.17333.236 1.00 80.98 6
ATOM 6861 CG1 VAL C 427 56.744 105.17433.375 1.00 80.25 6
ATOM 6862 CG2 VAL C 427 58.921 105.65532.294 1.00 81.07 6
ATOM 6863 C VAL C 427 56.307 108.00733.714 1.00168.67 6
ATOM 6864 O VAL C 427 55.355 108.69033.335 1.00169.83 8
ATOM 6865 N ARG C 428 56.479 107.64334.982 1.00 89.40 7
ATOM 6866 CA ARG C 428 55.540 108.05336.013 1.00 88.38 6
ATOM 6867 CB ARG C 428 56.003 107.52437.372 1.00 92.50 6
ATOM 6868 CG ARG C 428 56.396 106.05337.278 1.00 93.10 6
ATOM 6869 CD ARG C 428 56.368 105.31638.606 1.00 93.12 6
ATOM 6870 NE ARG C 428 56.960 103.98438.474 1.00 93.43 7
ATOM 6871 CZ ARG C 428 56.875 103.02439.390 1.00 93.85 6
ATOM 6872 NH1 ARG C 428 56.214 103.23540.518 1.00 94.45 7
ATOM 6873 NH2 ARG C 428 57.460 101.85539.184 1.00 94.29 7
ATOM 6874 C ARG C 428 55.473 109.57535.974 1.00 87.64 6
ATOM 6875 O ARG C 428 56.251 110.20635.256 1.00 87.70 8
ATOM 6876 N ASP C 429 54.552 110.16136.731 1.00110.57 7
ATOM 6877 CA ASP C 429 54.346 111.61236.735 1.00110.32 6
ATOM 6878 CB ASP C 429 55.643 112.41336.476 1.00112.49 6
ATOM 6879 CG ASP C 429 56.819 111.95737.332 1.00113.10 6
123

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ATOM 6880 OD1 ASP C 429 56.609 111.59538.508 1.00113.98 8
ATOM 6881 OD2 ASP C 429 57.963 111.98336.823 1.00112.70 8
ATOM 6882 C ASP C 429 53.393 111.86135.566 1.00109.53 6
ATOM 6883 0 ASP C 429 52.361 112.51635.712 1.00109.81 8
ATOM 6884 N VAL C 430 53.762 111.32934.402 1.00111.98 7
ATOM 6885 CA VAL C 430 52.964 111.45933.187 1.00110.45 6
ATOM 6886 CB VAL C 430 53.655 110.79231.979 1.00 68.68 6
ATOM 6887 CG1 VAL C 430 52.653 110.54330.862 1.00 67.72 6
ATOM 6888 CG2 VAL C 430 54.761 111.68931.478 1.00 69.29 6
ATOM 6889 C VAL C 430 51.627 110.78933.389 1.00109.91 6
ATOM 6890 0 VAL C 430 50.587 111.44933.363 1.00110.52 8
ATOM 6891 N HIS C 431 51.671 109.47233.579 1.00 80.56 7
ATOM 6892 CA HIS C 431 50.471 108.67233.797 1.00 78.57 6
ATOM 6893 CB HIS C 431 50.774 107.54734.797 1.00 58.67 6
ATOM 6894 CG HIS C 431 51.914 106.65434.394 1.00 55.89 6
ATOM 6895 CD2 HIS C 431 52.648 105.77135.113 1.00 55.43 6
ATOM 6896 ND1 HIS C 431 52.351 106.53833.092 1.00 54.61 7
ATOM 6897 CE1 HIS C 431 53.300 105.61933.027 1.00 53.93 6
ATOM 6898 NE2 HIS C 431 53.499 105.13834.239 1.00 53.95 7
ATOM 6899 C HIS C 431 49.320 109.54534.328 1.00 78.25 6
ATOM 6900 0 HIS C 431 48.240 109.60233.731 1.00 78.17 8
ATOM 6901 N ARG C 432 49.589 110.23235.441 1.00 46.08 7
ATOM 6902 CA ARG C 432 48.640 111.12036.121 1.00 46.27 6
ATOM 6903 CB ARG C 432 49.373 112.33436.700 1.00180.77 6
ATOM 6904 CG ARG C 432 50.103 112.06538.008 1.00183.68 6
ATOM 6905 CD ARG C 432 50.796 113.32138.522 1.00186.25 6
ATOM 6906 NE ARG C 432 51.412 113.11239.830 1.00189.10 7
ATOM 6907 CZ ARG C 432 52.165 114.01340.453 1.00191.25 6
ATOM 6908 NH1 ARG C 432 52.398 115.19039.883 1.00191.83 7
ATOM 6909 NH2 ARG C 432 52.683 113.74541.646 1.00192.15 7
ATOM 6910 C ARG C 432 47.473 111.61435.294 1.00 45.55 6
ATOM 6911 0 ARG C 432 47.605 111.89734.105 1.00 45.00 8
ATOM 6912 N THR C 433 46.324 111.70735.951 1.00 44.22 7
ATOM 6913 CA THR C 433 45.118 112.18635.312 1.00 44.25 6
ATOM 6914 CB THR C 433 43.924 112.26436.301 1.00 50.03 6
ATOM 6915 OG1 THR C 433 43.121 111.09236.165 1.00 49.50 8
ATOM 6916 CG2 THR C 433 43.054 113.47436.021 1.00 49.59 6
ATOM'6917 C THR C 433 45.404 113.57434.776 1.00 45.40 6
ATOM 6918 0 THR C 433 45.919 114.44735.486 1.00 45.43 8
ATOM 6919 N HIS C 434 45.075 113.75633.504 1.00156.27 7
ATOM 6920 CA HIS C 434 45.254 115.02532.826 1.00157.07 6
ATOM 6921 CB HIS C 434 45.573 114.80031.341 1.00144.78 6
ATOM 6922 CG HIS C 434 44.764 113.71630.691 0.00144.14 6
ATOM 6923 CD2 HIS C 434 43.760 112.93831.158 0.00143.69 6
ATOM 6924 ND1 HIS C 434 44.982 113.31929.389 0.00143.69 7
ATOM 6925 CE1 HIS C 434 44.147 112.34229.084 0.00143.54 6
ATOM 6926 NE2 HIS C 434 43.394 112.09130.139 0.00143.54 7
ATOM 6927 C HIS C 434 43.980 115.83032.970 1.00157.44 6
ATOM 6928 0 HIS C 434 42.935 115.44332.444 1.00158.31 8
ATOM 6929 N TYR C 435 44.071 116.94133.697 1.00 67.96 7
ATOM 6930 CA TYR C 435 42.922 117.81133.915 1.00 67.85 6
ATOM 6931 CB TYR C 435 43.379 119.16934.456 1.00 79.02 6
ATOM 6932 CG TYR C 435 44.815 119.51634.117 0.00 78.21 6
ATOM 6933 CD1 TYR C 435 45.238 119.61232.792 0.00 77.88 6
ATOM 6934 CE1 TYR C 435 46.565 119.90732.477 0.00 77.84 6
ATOM 6935 CD2 TYR C 435 45.755 119.72635.125 0.00 77.88 6
124

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ATOM 6936 CE2 TYR C 435 47.084 120.02234.823 0.00 77.84
6
ATOM 6937 CZ TYR C 435 47.482 120.10933.498 0.00 77.79
6
ATOM 6938 OH TYR C 435 48.796 120.39033.199 0.00 78.17
8
ATOM 6939 C TYR C 435 42.102 117.97832.636 1.00 68.74
6
ATOM 6940 0 TYR C 435 40.948 118.40532.685 1.00 69.03
8
ATOM 6941 N GLY C 436 42.712 117.65031.495 1.00 67.62
7
ATOM 6942 CA GLY C 436 42.007 117.69230.226 1.00 68.62
6
ATOM 6943 C GLY C 436 41.179 116.40730.171 1.00 69.36
6
ATOM 6944 0 GLY C 436 41.598 115.41229.585 1.00 72.11
8
ATOM 6945 N ARG C 437 40.007 116.42430.803 1.00 55.47
7
ATOM 6946 CA ARG C 437 39.119 115.26330.855 1.00 52.65
6
ATOM 6947 CB ARG C 437 38.202 115.37532.070 1.00 51.14
6
ATOM 6948 CG ARG C 437 37.287 116.57732.054 1.00 50.84
6
ATOM 6949 CD ARG C 437 36.395 116.60233.275 1.00 51.08
6
ATOM 6950 NE ARG C 437 35.064 117.07132.919 1.00 52.43
7
ATOM 6951 CZ ARG C 437 34.135 117.41133.800 1.00 53.50
6
ATOM 6952 NH1 ARG C 437 34.389 117.33735.096 1.00 53.97
7
ATOM 6953 NH2 ARG C 437 32.953 117.83233.385 1.00 54.15
7
ATOM 6954 C ARG C 437 38.269 115.00429.602 1.00 52.18
6
ATOM 6955 O ARG C 437 37.055 115.24329.574 1.00 52.84
8
ATOM 6956 N ILE C 438 38.928 114.48628.574 1.00 74.85
7
ATOM 6957 CA ILE C 438 38.304 114.16127.298 1.00 73.00
6
ATOM 6958 CB ILE C 438 38.672 115.19526.233 1.00 47.80
6
ATOM 6959 CG2 ILE C 438 37.485 115.43525.387 1.00 49.03
6
ATOM 6960 CG1 ILE C 438 39.127 116.51926.868 1.00 47.22
6
ATOM 6961 CD1 ILE C 438 39.368 117.66725.873 1.00 44.57
6
ATOM 6962 C ILE C 438 38.913 112.82026.900 1.00 72.42
6
ATOM 6963 O ILE C 438 39.014 112.45825.730 1.00 73.30
8
ATOM 6964 N CYS C 439 39.323 112.10227.931 1.00 77.21
7
ATOM 6965 CA CYS C 439 39.978 110.81727.830 1.00 75.33
6
ATOM 6966 CB CYS C 439 41.477 111.08627.736 1.00 47.34
6
ATOM 6967 SG CYS C 439 42.633 109.72327.743 1.00 37.84
16
ATOM 6968 C CYS C 439 39.591 110.19129.165 1.00 76.68
6
ATOM 6969 O CYS C 439 39.559 110.87330.183 1.00 77.75
8
ATOM 6970 N PRO C 440 39.304 108.88529.180 1.00 34.22
7
ATOM 6971 CD PRO C 440 39.890 107.98428.176 1.00105.10
6
ATOM 6972 CA PRO C 440 38.896 108.11730.369 1.00 34.23
6
ATOM 6973 CB PRO C 440 39.057 106.67829.912 1.00105.80
6
ATOM 6974 CG PRO C 440 40.237 106.77029.007 1.00107.40
6
ATOM 6975 C PRO C 440 39.708 108.40131.610 1.00 34.11
6
ATOM 6976 O PRO C 440 40.929 108.48631.545 1.00 33.92
8
ATOM 6977 N VAL C 441 39.034 108.52232.744 1.00 55.14
7
ATOM 6978 CA VAL C 441 39.731 108.81633.981 1.00 57.66
6
ATOM 6979 CB VAL C 441 38.906 109.78834.840 1.00 86.60
6
ATOM 6980 CG1 VAL C 441 38.932 111.16234.223 1.00 86.91
6
ATOM 6981 CG2 VAL C 441 37.473 109.31034.929 1.00 87.90
6
ATOM 6982 C VAL C 441 40.103 107.58834.816 1.00 59.20
6
ATOM 6983 O VAL C 441 40.921 107.69035.736 1.00 59.36
8
ATOM 6984 N GLU C 442 39.525 106.43034.491 1.00107.21
7
ATOM 6985 CA GLU C 442 39.809 105.19935.235 1.00108.59
6
ATOM 6986 CB GLU C 442 38.748 104.99636.311 1.00 86.62
6
ATOM 6987 CG GLU C 442 37.343 104.87935.746 1.00 87.79
6
ATOM 6988 CD GLU C 442 36.302 104.51336.795 1.00 89.95
6
ATOM 6989 OE1 GLU C 442 36.533 103.54837.551 1.00 92.77
8
ATOM 6990 OE2 GLU C 442 35.245 105.17836.861 1.00 89.95
8
ATOM 6991 C GLU C 442 39.860 103.94834.353 1.00110.16
6
125

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ATOM 6992 0 GLU C 442 39.150 102.98134.617 1.00111.15
8
ATOM 6993 N THR C 443 40.713 103.95733.328 1.00128.30
7
ATOM 6994 CA THR C 443 40.832 102.83032.394 1.00129.11
6
ATOM 6995 CB THR C 443 40.634 103.30030.929 1.00139.96
6
ATOM 6996 OG1 THR C 443 39.325 103.85430.775 1.00139.87
8
ATOM 6997 CG2 THR C 443 40.777 102.13829.969 1.00141.01
6
ATOM 6998 C THR C 443 42.125 101.99932.422 1.00129.49
6
ATOM 6999 O THR C 443 42.080 100.78332.247 1.00130.53
8
ATOM 7000 N PRO C 444 43.288 102.63932.637 1.00 77.51
7
ATOM 7001 CD PRO C 444 43.423 104.0'2433.120 1.00 66.72
6
ATOM 7002 CA PRO C 444 44.590 101.95932.675 1.00 78.03
6
ATOM 7003 CB PRO C 444 45.446 102.90833.507 1.00 67.09
6
ATOM 7004 CG PRO C 444 44.922 104.22833.138 1.00 66.86
6
ATOM 7005 C PRO C 444 44.581 100.56033.270 1.00 78.64
6
ATOM 7006 0 PRO C 444 43.617 100.16233.918 1.00 80.74
8
ATOM 7007 N GLU C 445 45.657 99.812 33.053 1.00 35.81
7
ATOM 7008 CA GLU C 445 45.733 98.482 33.634 1.00 35.36
6
ATOM 7009 CB GLU C 445 45.944 97.440 32.537 1.00 77.62
6
ATOM 7010 CG GLU C 445 44.692 96.612 32.246 1.00 79.75
6
ATOM 7011 CD GLU C 445 44.799 95.175 32.736 1.00 80.05
6
ATOM 7012 OE1 GLU C 445 45.667 94.434 32.235 1.00 80.64
8
ATOM 7013 OE2 GLU C 445 44.015 94.784 33.629 1.00 81.30
8
ATOM 7014 C GLU C 445 46.796 98.339 34.751 1.00 34.05
6
ATOM 7015 0 GLU C.445 46.697 98.974 35.797 1.00 34.45
8
ATOM 7016 N GLY C 446 47.799 97.493 34.554 1.00107.56
7
ATOM 7017 CA GLY C 446 48.804 97.328 35.593 1.00107.12
6
ATOM 7018 C GLY C 446 50.242 97.318 35.103 1.00106.84
6
ATOM 7019 0 GLY-C 446 50.555 96.730 34.062 1.00108.10
8
ATOM 7020 N ALA C 447 51.122 97.972 35.857 1.00 30.72
7
ATOM 7021 CA ALA C 447 52.540 98.037 35.513 1.00 28.70
6
ATOM 7022 CB ALA C 447 53.145 96.629 35.534 1.00121.82
6
ATOM 7023 C ALA C 447 52.753 98.689 34.145 1.00 27.10
6
ATOM 7024 O ALA C 447 53.863 98.708 33.607 1.00 26.16
8
ATOM 7025 N ASN C 448 51.670 99.231 33.605 1.00 30.81
7
ATOM 7026 CA ASN C 448 51.650 99.891 32.305 1.00 29.38
6
ATOM 7027 CB ASN C 448 51.067 98.926 31.261 1.00 61.60
6
ATOM 7028 CG ASN C 448 51.307 99.381 29.845 1.00 64.54
6
ATOM 7029 OD1 ASN C 448 51.317 100.56729.561 1.00 68.29
8
ATOM 7030 ND2 ASN C 448 51.487 98.435 28.944 1.00 65.27
7
ATOM 7031 C ASN C 448 50.719 101.10232.466 1.00 27.57
6
ATOM 7032 O ASN C 448 50.221 101.64431.487 1.00 26.46
8
ATOM 7033 N ILE C 449 50.486 101.50533.716 1.00 71.41
7
ATOM 7034 CA ILE C 449 49.587 102.61534.059 1.00 71.54
6
ATOM 7035 CB ILE C 449 49.902 103.16035.467 1.00138.02
6
ATOM 7036 CG2 ILE C 449 48.723 103.95635.986 1.00138.57
6
ATOM 7037 CG1 ILE C 449 50.209 102.00436.422 1.00138.34
6
ATOM 7038 CD1 ILE C 449 49.134 100.93936.475 1.00139.38
6
ATOM 7039 C ILE C 449 49.627 103.79233.087 1.00 70.75
6
ATOM 7040 0 ILE C 449 50.579 103.94632.329 1.00 71.98
8
ATOM 7041 N GLY C 450 48.579 104.61433.116 1.00 82.97
7
ATOM 7042 CA GLY C 450 . 48.502 105.79832.268 1.00 80.93
6
ATOM 7043 C GLY C 450 48.743 105.64830.778 1.00 79.00
6
ATOM 7044 0 GLY C 450 47.830 105.81529.976 1.00 79.47
8
ATOM 7045 N LEU C 451 49.982 105.35930.405 1.00 53.93
7
ATOM 7046 CA LEU C 451 50.367 205.18629.007 1.00 52.47
6
ATOM 7047 CB LEU C 451 51.494 104.17328.908 1.00 30.07
6
126

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ATOM 7048 CG LEU C 451 52.689 104.41029.809 1.00 29.20
ATOM 7049 CD1 LEU C 451 53.644 103.26529.605 1.00 28.64
ATOM 7050 CD2 LEU C 451 53.342 105.74329.498 1.00 28.84
ATOM 7051 C LEU C 451 49.237 104.72428.093 1.00 52.53
ATOM 7052 0 LEU C 451 49.004 105.30327.033 1.00 52.57
ATOM 7053 N ILE C 452 48.547 103.66728.505 1.00 37.93
ATOM 7054 CA ILE C 452 47.466 103.11527.713 1.00 39.02
ATOM 7055 CB ILE C 452 47.519 101.62127.717 1.00 49.63
ATOM 7056 CG2 ILE C 452 46.469 101.08826.781 1.00 51.01
ATOM 7057 CG1 ILE C 452 48.910 101.16727.300 1.00 51.02
ATOM 7058 CD1 ILE C 452 49.150 99.705 27.517 1.00 52.72
ATOM 7059 C ILE C 452 46.094 103.51528.200 1.00 39.38
ATOM 7060 0 ILE C 452 45.488 102.82629.012 1.00 40.50
ATOM 7061 N THR C 453 45.591 104.62127.680 1.00 42.99
ATOM 7062 CA THR C 453 44.286 105.10428.083 1.00 43.18
ATOM 7063 CB THR C 453 44.306 106.62828.190 1.00 78.48
ATOM 7064 OG1 THR C 453 42.988 107.10028.474 1.00 82.01
ATOM 7065 CG2 THR C 453 44.820 107.24826.898 1.00 77.30
ATOM 7066 C THR C 453 43.214 104.67527.093 1.00 43.15
ATOM 7067 O THR C 453 43.518 104.03926.077 1.00 43.49
ATOM 7068 N SER C 454 41.963 105.01727.397 1.00 45.38
ATOM 7069 CA SER C 454 40.850 104.67426.524 1.00 45.94
ATOM 7070 CB SER C 454 39.728 104.01627.325 1.00 81.44
ATOM 7071 OG SER C 454 38.829 103.33726.460 1.00 86.20
ATOM 7072 C SER C 454 40.330 105.91625.801 1.00 45.54
ATOM 7073 0 SER C 454 41.045 106.49724.995 1.00 46.42
ATOM 7074 N LEU C 455 39.099 106.33926.076 1.00 48.01
ATOM 7075 CA LEU C 455 38.560 107.51325.396 1.00 47.92
ATOM 7076 CB LEU C 455 38.567 107.27723.889 1.00 34.19
ATOM 7077 CG LEU C 455 38.176 108.51023.095 1.00 35.88
ATOM 7078 CD1 LEU C 455 39.200 109.57023.404 1.00 36.69
ATOM 7079 CD2 LEU C 455 38.119 108.23221.613 1.00 35.89
ATOM 7080 C LEU C 455 37.146 107.88925.835 1.00 47.75
ATOM 7081 O LEU C 455 36.266 107.03325.900 1.00 47.58
ATOM 7082 N ALA C 456 36.938 109.17726.118 1.00 47.48
ATOM 7083 CA ALA C 456 35.633 109.70226.551 1.00 46.84
ATOM 7084 CB ALA C 456 35.653 111.22626.601 1.00 16.72
ATOM 7085 C ALA C 456 34.543 109.26225.616 1.00 46.95
ATOM 7086 O ALA C 456 34.716 109.28924.402 1.00 47.71
ATOM 7087 N ALA C 457 33.408 108.88326.177 1.00 39.38
ATOM 7088 CA ALA C 457 32.315 108.43325.350 1.00 39.45
ATOM 7089 CB ALA C 457 31.103 108.21126.187 1.00 13.87
ATOM 7090 C ALA C 457 32.023 109.44124.263 1.00 39.11
ATOM 7091 O ALA C 457 32.367 109.25023.105 1.00 39.8'7
ATOM 7092 N TYR C 458 31.403 110.53524.648 1.00 36.08
ATOM 7093 CA TYR C 458 31.044 111.56523.701 1.00 37.92
ATOM 7094 CB TYR C 458 30.203 112.61724.412 1.00 33.09
ATOM 7095 CG TYR C 458 28.740 112.30524.598 1.00 31.68
ATOM 7096 CD1 TYR C 458 28.315 111.16125.264 1.00 31.59
ATOM 7097 CE1 TYR C 458 26.974 110.91725.476 1.00 30.71
ATOM 7098 CD2 TYR C 458 27.782 113.19124.150 1.00 31.70
ATOM 7099 CE2 TYR C 458 26.458 112.96324.354 1.00 31.63
ATOM 7100 CZ TYR C 458 26.052 111.83225.013 1.00 31.31
ATOM 7101 OH TYR C 458 24.705 111.62725.183 1.00 32.83
ATOM 7102 C TYR C 458 32.224 112.27323.031 1.00 40.86
ATOM 7103 O TYR C 458 31.997 113.15822.206 1.00 41.46
127

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 7104 N ALA C 459 33.460 111.89423.372 1.00 61.61
7
ATOM 7105 CA ALA C 459 34.681 112.54122.852 1.00 63.60
6
ATOM 7106 CB ALA C 459 35.901 111.92623.525 1.00181.11
6
ATOM 7107 C ALA C 459 34.948 112.64321.349 1.00 64.59
6
ATOM 7108 0 ALA C 459 34.663 111.73320.572 1.00 65.49
8
ATOM 7109 N ARG C 460 35.532 113.77220.967 1.00 66.70
7
ATOM 7110 CA ARG C 460 35.904 114.07819.588 1.00 68.41
6
ATOM 7111 CB ARG C 460 37.184 113.34219.205 1.00 52.09
6
ATOM 7112 CG ARG C 460 37.435 113.40617.721 1.00 50.99
6
ATOM 7113 CD ARG C 460 38.894 113.45517.377 1.00 50.98
6
ATOM 7114 NE ARG C 460 39.647 112.36017.975 1.00 52.48
7
ATOM 7115 CZ ARG C 460 40.920 112.08617.692 1.00 52.62
6
ATOM 7116 NH1 ARG C 460 41.574 112.83016.811 1.00 51.72
7
ATOM 7117 NH2 ARG C 460 41.547 111.08218.302 1.00 53.37
7
ATOM 7118 C ARG C 460 34.921 113.89118.446 1.00 70.46
6
ATOM 7119 O ARG C 460 34.249 112.86518.349 1.00 70.85
8
ATOM 7120 N VAL C 461 34.905 114.89817.565 1.00174.70
7
ATOM 7121 CA VAL C 461 34.087 114.96816.346 1.00175.02
6
ATOM 7122 CB VAL C 461 32.671 114.37116.535 1.00 46.89
6
ATOM 7123 CG1 VAL C 461 31.623 115.26315.908 1.00 47.41
6
ATOM 7124 CG2 VAL C 461 32.607 113.03915.853 1.00 45.72
6
ATOM 7125 C VAL C 461 33.947 116.40115.824 1.00175.88
6
ATOM 7126 O VAL C 461 33.629 117.31416.586 1.00176.25
8
ATOM 7127 N ALA C 462 34.181 116.56414.519 1.00122.51
7
ATOM 7128 CA ALA C 462 34.112 117.84513.796 1.00123.73
6
ATOM 7129 CB ALA C 462 33.877 119.00514.752 1.00 28.29
6
ATOM 7130 C ALA C 462 35.426 118.05213.046 1.00125.07
6
ATOM 7131 0 ALA C 462 36.335 117.22813.165 1.00125.53
8
ATOM 7132 N ALA C 463 35.527 119.13712.273 1.00128.67
7
ATOM 7133 CA ALA C 463 36.752 119.42311.522 1.00128.13
6
ATOM 7134 CB ALA C 463 36.757 120.88711.040 1.00 50.08
6
ATOM 7135 C ALA C 463 37.923 119.14712.469 1.00127.82
6
ATOM 7136 O ALA C 463 38.264 129.97813.318 1.00128.71
8
ATOM 7137 N LEU C 464 38.525 117.96712.313 1.00 54.63
7
ATOM 7138 CA LEU C 464 39.616 117.50713.185 1.00 54.40
6
ATOM 7139 CB LEU C 464 39.453 116.00813.435 1.00112.52
6
ATOM 7140 CG LEU C 464 40.430 115.41914.447 1.00113.36
6
ATOM 7141 CD1 LEU C 464 40.184 116.03515.817 1.00114.51
6
ATOM 7142 CD2 LEU C 464 40.258 113.91314.487 1.00114.04
6
ATOM 7143 C LEU C 464 41.089 117.78612.838 1.00 53.28
6
ATOM 7144 0 LEU C 464 41.760 117.00012.154 1.00 51.53
8
ATOM 7145 N GLY C 465 41.581 118.91213.348 1.00 98.23
7
ATOM 7146 CA GLY C 465 42.966 129.27813.148 1.00 96.83
6
ATOM 7147 C GLY C 465 43.717 118.42614.147 1.00 96.13
6
ATOM 7148 0 GLY C 465 44.832 117.98013.879 1.00 95.71
8
ATOM 7149 N PHE C 466 43.074 118.19615.298. 1.00 84.96
7
ATOM 7150 CA PHE C 466 43.628 117.37816.383 1.00 82.91
6
ATOM 7151 CB PHE C 466 45.070 117.81116.710 1.00 75.66
6
ATOM 7152 CG PHE C 466 45.199 119.20117.341 1.00 75.74
6
ATOM 7153 CD1 PHE C 466 44.847 119.42118.669 1.00 75.04
6
ATOM 7154 CD2 PHE C 466 45.756 120.26516.619 1.00 75.64
6
ATOM 7155 CE1 PHE C 466 45.052 120.65619.272 1.00 75.12
6
ATOM 7156 CE2 PHE C 466 45.962 121.51117.220 1.00 74.81
6
ATOM 7157 CZ PHE C 466 45.612 121.70218.547 1.00 75.38
6
ATOM 7158 C PHE C 466 42.810 117.34817.677 1.00 81.00
6
ATOM 7159 0 PHE C 466 41.709 117.89017.756 1.00 80.79
8
12~

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 7160 N ILE C 467 43.395 116.681 18.667 1.00 42.94
ATOM 7161 CA ILE C 467 42.888 116.482 20.033 1.00 41.29
ATOM 7162 CB ILE C 467 43.575 117.476 20.973 1.00110.42
ATOM 7163 CG2 ILE C 467 43.261 117.123 22.413 1.00109.50
ATOM 7164 CG1 ILE C 467 45.090 117.367 20.775 1.00112.81
ATOM 7165 CD1 ILE C 467 45.907 118.427 21.464 1.00116.18
ATOM 7166 C ILE C 467 41.403 116.366 20.449 1.00 38.46
ATOM 7167 O ILE C 467 40.613 117.291 20.366 1.00 37.83
ATOM 7168 N ARG C 468 41.089 115.184 20.960 1.00 64.17
ATOM 7169 CA ARG C 468 39.781 114.749 21.437 1.00 61.14
ATOM 7170 CB ARG C 468 39.993 113.970 22.723 1.00 65.30
ATOM 7171 CG ARG C 468 41.068 112.935 22.573 1.00 63.34
ATOM 7172 CD ARG C 468 41.819 112.761 23.857 1.00 62.63
ATOM 7173 NE ARG C 468 42.908 111.817 23.679 1.00 62.42
ATOM 7174 CZ ARG C 468 43.681 111.375 24.662 1.00 62.87
ATOM 7175 NH1 ARG C 468 43.487 111.796 25.906 1.00 62.17
ATOM 7176 NH2 ARG C 468 44.647 110.505 24.398 1.00 62.03
ATOM 7177 C ARG C 468 38.578 115.666 21.618 1.00 59.37
ATOM 7178 O ARG C 468 37.467 115.218 21.389 1.00 59.52
ATOM 7179 N THR C 469 38.762 116.911 22.046 1.00 28.37
ATOM 7180 CA THR C 469 37.618 117.830 22.247 1.00 27.33
ATOM 7181 CB THR C 469 37.348 118.700 20.995 1.00 77.07
ATOM 7182 OG1 THR C 469 36.958 117.865 19.905 1.00 77.37
ATOM 7183 CG2 THR C 469 38.578 119.489 20.600 1.00 77.50
ATOM 7184 C THR C 469 36.261 117.182 22.627 1.00 25.69
ATOM 7185 O THR C 469 35.666 116.455 21.828 1.00 25.93
ATOM 7186 N PRO C 470 35.737 117.473 23.842 1.00 39.55
ATOM 7187 CD PRO C 470 36.260 118.385 24.884 1.00 24.20
ATOM 7188 CA PRO C 470 34.457 116.892 24.268 1.00 38.89
ATOM 7189 CB PRO C 470 34.534 116.963 25.779 1.00 22.75
ATOM 7190 CG PRO C 470 35.172 118.308 25.980 1.00 22.38
ATOM 7191 C PRO C 470 33.304 117.716 23.741 1.00 39.28
ATOM 7192 0 PRO C 470 33.425 118.405 22.726 1.00 41.11
ATOM 7193 N TYR C 471 32.182 117.654 24.437 1.00 32.55
ATOM 7194 CA TYR C 471 31.016 118.403 24.025 1.00 34.52
ATOM 7195 CB TYR C 471 29.879 117.433 23.683 1.00 75.03
ATOM 7196 CG TYR C 471 30.055 116.756 22.333 1.00 77.73
ATOM 7197 CD1 TYR C 471 31.199 116.012 22.041 1.00 77.86
ATOM 7198 CE1 TYR C 471 31.398 115.467 20.777 1.00 78.14
ATOM 7199 CD2 TYR C 471 29.109 116.923 21.319 1.00 78.93
ATOM 7200 CE2 TYR C 471 29.305 116.376 20.052 1.00 78.86
ATOM 7201 CZ TYR C 471 30.449 115.656 19.794 1.00 78.86
ATOM 7202 OH TYR C 471 30.646 115.138 18.543 1.00 80.15
ATOM 7203 C TYR C 471 30.626 119.316 25.162 1.00 35.18
ATOM 7204 O TYR C 471 30.895 119.013 26.321 1.00 35.81
ATOM 7205 N ARG C 472 30.017 120.450 24.841 1.00 59.06
ATOM 7206 CA ARG C 472 29.589 121.380 25.876 1.00 59.21
ATOM 7207 CB ARG C 472 30.739 122.306 26.280 1.00 47.34
ATOM 7208 CG ARG C 472 32.014 121.578 26.692 1.00 46.47
ATOM 7209 CD ARG C 472 32.857 122.380 27.671 1.00 46.11
ATOM 7210 NE ARG C 472 34.206 121.839 27.775 1.00 47.37
ATOM 7211 CZ ARG C 472 34.486 120.594 28.139 1.00 47.44
ATOM 7212 NH1 ARG C 472 33.499 119.766 28.436 1.00 46.95
ATOM 7213 NH2 ARG C 472 35.748 120.175 28.196 1.00 47.86
ATOM 7214 C ARG C 472 28.403 122.210 25.414 1.00 60.34
ATOM 7215 O ARG C 472 28.386 122.726 24.301 1.00 61.28
129

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 7216 N ARG C 473 27.403 122.33026.272 1.00 55.07
ATOM 7217 CA ARG C 473 26.227 123.11525.943 1.00 56.77
ATOM 7218 CB ARG C 473 25.078 122.72326.869 1.00 63.20
ATOM 7219 CG ARG C 473 23.718 122.85726.235 1.00 63.69
ATOM 7220 CD ARG C 473 22.651 122.17927.073 1.00 64.69
ATOM 7221 NE ARG C 473 ' 21.331 122.30926.459 1.00 66.59
ATOM 7222 CZ ARG C 473 20.197 121.87727.007 1.00 67.61
ATOM 7223 NH1 ARG C 473 20.222 121.27828.194 1.00 68.38
ATOM 7224 NH2 ARG C 473 19.035 122.05526.378 1.00 66.88
ATOM 7225 C ARG C 473 26.575 124.60426.091 1.00 58.13
ATOM 7226 0 ARG C 473 27.644 124.95626.584 1.00 58.42
ATOM 7227 N VAL C 474 25.678 125.47425.651 1.00 43.60
ATOM 7228 CA VAL C 474 25.880 126.92425.700 1.00 45.65
ATOM 7229 CB VAL C 474 26.764 127.41024.510 1.00105.12
ATOM 7230 CG1 VAL C 474 26.961 128.91924.573 1.00107.36
ATOM 7231 CG2 VAL C 474 28.103 126.69424.511 1.00104.39
ATOM 7232 C VAL C 474 24.482 127.50525.517 1.00 46.42
ATOM 7233 O VAL C 474 24.228 128.28824.591 1.00 46.13
ATOM 7234 N ALA C 475 23.572 127.08526.390 1.00 68.26
ATOM 7235 CA ALA C 475 22.171 127.50726.346 1.00 69.34
ATOM 7236 CB ALA C 475 22.074 129.01726.224 1.00168.36
ATOM 7237 C ALA C 475 21.429 126.83725.188 1.00 69.26
ATOM 7238 O ALA C 475 21.237 127.43124.121 1.00 67.34
ATOM 7239 N ALA C 476 21.031 125.59025.422 1.00 62.24
ATOM 7240 CA ALA C 476 20.295 124.78424.455 1.00 64.22
ATOM 7241 CB ALA C 476 18.887 125.33724.294 1.00146.64
ATOM 7242 C ALA C 476 20.969 124.69023.098 1.00 65.26
ATOM 7243 O ALA C 476 20.937 123.64022.453 1.00 64.43
ATOM 7244 N GLY C 477 21.561 125.80222.677 1.00116.21
ATOM 7245 CA GLY C 477 22.239 125.89021.399 1.00118.42
ATOM 7246 C GLY C 477 22.437 124.57420.684 1.00118.96
ATOM 7247 O GLY C 477 21.511 124.03420.075 1.00119.12
ATOM 7248 N ALA C 478 23.654 124.05720.761 1.00 84.50
ATOM 7249 CA ALA C 478 23.970 122.80320.109 1.00 85.41
ATOM 7250 CB ALA C 478 24.664 123.06518.782 1.00170.39
ATOM 7251 C ALA C 478 24.859 121.96221.001 1.00 85.57
ATOM 7252 0 ALA C 478 24.391 121.07421.704 1.00 84.87
ATOM 7253 N VAL C 479 26.149 122.24720.966 1.00 65.43
ATOM 7254 CA VAL C 479 27.096 121.50521.766 1.00 66.60
ATOM 7255 CB VAL C 479 27.087 120.01221.413 1.00 80.06
ATOM 7256 CG1 VAL C 479 26.146 119.25922.325 1.00 80.61
ATOM 7257 CG2 VAL C 479 26.686 119.83519.963 1.00 80.04
ATOM 7258 C VAL C 479 28.484 122.03621.507 1.00 67.45
ATOM 7259 O VAL C 479 29.404 121.74822.266 1.00 67.56
ATOM 7260 N THR C 480 28.633 122.81420.439 1.00 38.12
ATOM 7261 CA THR C 480 29.930 123.37320.091 1.00 37.95
ATOM 7262 CB THR C 480 30.187 124.73520.803 1.00 59.69
ATOM 7263 OG1 THR C 480 31.528 125.17920.545 1.00 60.79
ATOM 7264. CG2 THR C 480 30.001 124.60522.300 1.00 59.28
ATOM 7265 C THR C 480 31.027 122.40720.495 1.00 38.63
ATOM 7266 0 THR C 480 31.440 122.37821.653 1.00 37.92
ATOM 7267 N GLU C 481 31.485 121.60119.546 1.00 70.47
ATOM 7268 CA GLU C 481 32.553 120.64919.819 1.00 72.47
ATOM 7269 CB GLU C 481 33.082 120.05618.512 1.00208.87
ATOM 7270 CG GLU C 481 32.010 119.70617.478 1.00208.87
ATOM 7271 CD GLU C 481 31.548 120.90716.661 1.00208.87
130

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ATOM 7272 OE1 GLU C 481 30.845 121.784 17.209 1.00208.87
~8
ATOM 7273 OE2 GLU C 481 31.893 120.978 15.461 1.00208.87
8
ATOM 7274 C GLU C 481 33.655 121.449 20.506 1.00 71.91
6
ATOM 7275 O GLU C 481 34.351 122.237 19.868 1.00 72.01
8
ATOM 7276 N ALA C 482 33.796 121.258 21.812 1.00 73.53
7
ATOM 7277 CA ALA C 482 34.796 121.982 22.580 1.00 74.20
6
ATOM 7278 CB ALA C 482 34.829 121.473 24.006 1.00 70.38
6
ATOM 7279 C ALA C 482 36.173 121.870 21.959 1.00 75.30
6
ATOM 7280 O ALA C 482 36.348 121.224 20.929 1.00 75.99
8
ATOM 7281 N ALA C 483 37.144 122.521 22.593 1.00132.12
7
ATOM 7282 CA ALA C 483 38.531 122.523 22.136 1.00132.18
6
ATOM 7283 CB ALA C 483 38.787 123.725 21.228 1.00145.75
6
ATOM 7284 C ALA C 483 39.430 122.585 23.366 1.00131.50
6
ATOM 7285 O ALA C 483 39.509 123.609 24.032 1.00131.95
8
ATOM 7286 N VAL C 484 40.106 121.477 23.645 1.00 42.28
7
ATOM 7287 CA VAL C 484 40.988 121.318 24.801 1.00 42.41
6
ATOM 7288 CB VAL C 484 42.096 120.318 24.498 1.00164.56
6
ATOM 7289 CG1 VAL C 484 42.856 120.000 25.774 1.00165.45
6
ATOM 7290 CG2 VAL C 484 41.492 119.061 23.901 1.00165.59
6
ATOM 7291 C VAL C 484 41.615 122.563 25.397 1.00 41.56
6
ATOM 7292 O VAL C 484 41.859 122.607 26.597 1.00 42.17
8
ATOM 7293 N ALA C 485 41.913 123.558 24.574 1.00 71.27
7
ATOM 7294 CA ALA C 485 42.455 124.804 25.097 1.00 70.95
6
ATOM 7295 CB ALA C 485 43.592 125.307 24.224 1.00 13.87
6
ATOM 7296 C ALA C 485 41.266 125.766 25.064 1.00 71.12
6
ATOM 7297 O ALA C 485 41.348 126.883 24.565 1.00 71.61
8
ATOM 7298 N MET C 486 40.143 125.290 25.587 1.00 78.07
7
ATOM 7299 CA MET C 486 38.918 126.067 25.625 1.00 77.99
6
ATOM 7300 CB MET C 486 37.701 125.149 25.473 1.00 78.50
6
ATOM 7301 CG MET C 486 36.382 125.870 25.255 1.00 78.76
6
ATOM 7302 SD MET C 486 35.061 124.774 24.651 1.00 77.29
16
ATOM 7303 CE MET C 486 35.149 125.096 22.898 1.00 77.76
6
ATOM 7304 C MET C 486 38.860 126.791 26.956 1.00 78.61
6
ATOM 7305 0 MET C 486 39.085 126.197 28.017 1.00 78.63
8
ATOM 7306 N ALA C 487 38.568 128.086 26.872 1.00157.96
7
ATOM 7307 CA ALA C 487 38.464 128.966 28.027 1.00157.73
6
ATOM 7308 CB ALA C 487 39.405 130.159 27.856 1.00 13.87
6
ATOM 7309 C ALA C 487 37.030 129.460 28.164 1.00157.88
6
ATOM 7310 0 ALA C 487 36.659 130.007 29.202 1.00158.59
8
ATOM 7311 N ALA C 488 36.238 129.255 27.109 1.00193.55
7
ATOM 7312 CA ALA C 488 34.841 129.689 27.065 1.00194.23
6
ATOM 7313 CB ALA C 488 33.970 128.658 26.290 1.00 20.13
6
ATOM 7314 C ALA C 488 34.273 129.936 28.462 1.00194.39
6
ATOM 7315 O ALA C 488 33.604 129.083 29.042 1.00195.34
8
ATOM 7316 N ALA C 489 34.579 131.124 28.981 1.00108.29
7
ATOM 7317 CA ALA C 489 34.156 131.609 30.292 1.00107.01
6
ATOM 7318 CB ALA C 489 33.173 132.745 30.111 1.00 21.82
6
ATOM 7319 C ALA C 489 33.582 130.606 31.281 1.00106.31
6
ATOM 7320 O ALA C 489 32.551 130.878 31.894 1.00106.68
8
ATOM 7321 N ALA C 490 34.243 129.463 31.447 1.00 94.59
7
ATOM 7322 CA ALA C 490 33.788 128.438 32.389 1.00 93.90
6
ATOM 7323 CB ALA C 490 33.302 129.098 33.676 1.00 13.87
6
ATOM 7324 C ALA C 490 32.699 127.506 31.861 1.00 93.34
6
ATOM 7325 O ALA C 490 31.927 127.859 30.972 1.00 95.08
8
ATOM 7326 N GLU C 491 32.652 126.307 32.427 1.00 45.09
7
ATOM 7327 CA GLU C 491 31.659 125.310 32.067 1.00 43.70
6
131

CA 02440409 2003-09-09
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ATOM 7328 CB GLU C 491 32.324 124.06831.460 1.00101.46
6
ATOM 7329 CG GLU C 491 33.573 123.59432.195 1.00103.12
6
ATOM 7330 CD GLU C 491 34.161 122.31431.612 1.00103.44
6
ATOM 7331 OE1 GLU C 491 34.108 122.15130.374 1.00103.66
8
ATOM 7332 OE2 GLU C 491 34.688 121.48132.388 1.00102.72
8
ATOM 7333 C GLU C 491 30.978 124.97233.381 1.00 43.30
6
ATOM 7334 0 GLU C 491 31.634 124.93834.422 1.00 43.64
8
ATOM 7335 N ALA C 492 29.666 124.74933.342 1.00 48.24
7
ATOM 7336 CA ALA C 492 28.905 124.44234.553 1.00 47.20
6
ATOM 7337 CB ALA C 492 27.734 125.39834.685 1.00126.11
6
ATOM 7338 C ALA C 492' 28.410 123.00934.563 1.00 47.18
6
ATOM 7339 0 ALA C 492 27.500 122.64733.813 1.00 47.47
8
ATOM 7340 N ARG C 493 29.016 122.21035.441 1.00 93.61
7
ATOM 7341 CA ARG C 493 28.694 120.79535.582 1.00 93.11
6
ATOM 7342 CB ARG C 493 29.323 10.229 36.863 1.00 61.11
6
ATOM 7343 CG ARG C 493 30.851 120.30336.838 1.00 58.90
6
ATOM 7344 CD ARG C 493 31.522 119.61638.026 1.00 57.23
6
ATOM 7345 NE ARG C 493 32.980 119.78537.999 1.00 54.51
7
ATOM 7346 CZ ARG C 493 33.820 119.25738.887 1.00 52.88
6
ATOM 7.347 NH1 ARG C 493 33.363 118.52139.885 1.00 51.83
7
ATOM 7348 NH2 ARG C 493 35.123 119.45638.772 1.00 51.80
7
ATOM 7349 C ARG C 493 27.208 120.50535.547 1.00 93.57
6
ATOM 7350 0 ARG C 493 26.394 121.26036.077 1.00 93.81
8
ATOM 7351 N TYR C 494 26.880 119.39634.895 1.00 57.29
7
ATOM 7352 CA TYR C 494 25.515 118.92634.713 1.00 58.47
6
ATOM 7353 CB TYR C 494 25.564 117.50534.177 1.00 80.31
6
ATOM 7354 CG TYR C 494 24.221 116.86033.938 1.00 81.49
6
ATOM 7355 CD1 TYR C 494 23.343 117.36832.982 1.00 81.70
6
ATOM 7356 CE1 TYR C 494 22.156 116.70532.680 1.00 82.02
6
ATOM 7357 CD2 TYR C 494 23.870 115.67734.599 1.00 82.19
6
ATOM 7358 CE2 TYR C 494 22.689 115.00934.304 1.00 82.33
6
ATOM 7359 CZ TYR C 494 21.844 115.52633.342 1.00 82.56
6
ATOM 7360 OH TYR C 494 20.709 114.84533.016 1.00 83.98
8
ATOM 7361 C TYR C 494 24.619 118.97535.950 1.00 59.88
6
ATOM 7362 0 TYR C 494 25.062 118.69037.073 1.00 60.16
8
ATOM 7363 N THR C 495 23.355 119.33435.706 1.00111.91
7
ATOM 7364 CA THR C 495 22.294 119.44636.711 1.00112.57
6
ATOM 7365 CB THR C 495 22.618 118.61037.978 1.00 98.93
6
ATOM 7366 OG1 THR C 495 23.125 117.32937.586 1.00 98.83
8
ATOM 7367 CG2 THR C 495 21.362 118.38538.807 1.00 99.57
6
ATOM 7368 C THR C 495 21.986 120.90437.107 1.00113.56
6
ATOM 7369 0 THR C 495 22.722 121.82936.743 1.00113.52
8
ATOM 7370 N ALA C 496 20.884 121.07537.845 1.00 85.32
7
ATOM 7371 CA ALA C 496 20.354 122.35838.333 1.00 85.17
6
ATOM 7372 CB ALA C 496 21.405 123.44338.301 1.00 64.23
6
ATOM 7373 C ALA C 496 19.213 122.72237.408 1.00 85.77
6
ATOM 7374 0 ALA C 496 18.605 123.78037.529 1.00 86.30
8
ATOM 7375 N ALA C 497 18.938 121.81036.483 1.00105.36
7
ATOM 7376 CA ALA C 497 17.895 121.96235.481 1.00106.23
6
ATOM 7377 CB ALA C 497 17.616 120.61134.831 1.00140.99
6
ATOM 7378 C ALA C 497 16.589 122.57935.969 1.00106.61
6
ATOM 7379 O ALA C 497 16.060 122.22337.024 1.00106.73
8
ATOM 7380 N ALA C 498 16.082 123.51635.178 1.00105.43
7
ATOM 7381 CA ALA C 498 14.827 124.20035.455 1.00106.36
6
ATOM 7382 CB ALA C 498 15.074 125.54736.146 1.00 32.90
6
ATOM 7383 C ALA C 498 14.207 124.40734.082 1.00106.88
6
132

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ATOM 7384 0 ALA C 498 14.027 125.53933.622 1.00107.64
8
ATOM 7385 N ALA C 499 13.907 123.28333.433 1.00111.52
7
ATOM 7386 CA ALA C 499 13.316 123.25932.101 1.00111.25
6
ATOM 7387 CB ALA C 499 12.043 124.10832.060 1.00120.72
6
ATOM 7388 C ALA C 499 14.327 123.77931.097 1.00110.44
6
ATOM 7389 0 ALA C 499 14.990 123.01030.403 1.00110.80
8
ATOM 7390 N ASN C 500 14.451 125.09531.036 1.00 43.61
7
ATOM 7391 CA ASN C 500 15.379 125.71730.115 1.00 43.08
6
ATOM 7392 CB ASN C 500 14.598 126.43829.010 1.00114.29
6
ATOM 7393 CG ASN C 500 13.934 125.48028.037 1.00115.71
6
ATOM 7394 OD1 ASN C 500 14.560 125.01327.087 1.00117.56
8
ATOM 7395 ND2 ASN C 500 12.662 125.17928.274 1.00115.42
7
ATOM 7396 C ASN C 500 16.288 126.71030.834 1.00 42.38
6
ATOM 7397 0 ASN C 500 17.383 127.00330.362 1.00 41.72
8
ATOM 7398 N THR C 501 15.850 127.20631.988 1.00 58.14
7
ATOM 7399 CA THR C 501 16.627 128.20632.725 1.00 58.92
6
ATOM 7400 CB THR C 501 15.698 129.36033.207 1.00174.72
6
ATOM 7401 OG1 THR C 501 14.803 129.73732.152 1.00176.53
8
ATOM 7402 CG2 THR C 501 16.517 130.58033.606 1.00175.70
6
ATOM 7403 C THR C 501 17.426 127.71333.940 1.00 57.69
6
ATOM 7404 0 THR C 501 18.193 128.47634.537 1.00 56.54
8
ATOM 7405 N PRO C 502 17.325 126.41834.267 1.00 51.85
7
ATOM 7406 CD PRO C 502 17.400 125.36733.236 1.00194.50
6
ATOM 7407 CA PRO C 502 18.070 125.96135.434 1.00 51.22
6
ATOM 7408 CB PRO C 502 19.315 125.37134.802 1.00194.09
6
ATOM 7409 CG PRO C 502 18.696 124.60133.620 1.00194.75
6
ATOM 7410 C PRO C 502 18.339 127.02336.485 1.00 50.18
6
ATOM 7411 0 PRO C 502 19.440 127.54936.603 1.00 49.51
8
ATOM 7412 N ALA C 503 17.302 127.34637.242 1.00 83.31
7
ATOM 7413 CA ALA C 503 17.443 128.32638.299 1.00 82.70
6
ATOM 7414 CB ALA C 503 16.079 128.70038.858 1.00157.97
6
ATOM 7415 C ALA C 503 18.287 127.64639.366 1.00 81.70
6
ATOM 7416 0 ALA C 503 17.863 126.65339.968 1.00 80.59
8
ATOM 7417 N ALA C 504 19.486 128.17639.587 1.00 61.52
7
ATOM 7418 CA ALA C 504 20.387 127.59940.565 1.00 61.35
~ 6
ATOM 7419 CB ALA C 504 21.666 127.15439.877 1.00150.60
6
ATOM 7420 C ALA C 504 20.723 128.53241.710 1.00 60.28
6
ATOM 7421 0 ALA C 504 20.310 128.30942.847 1.00 59.15
8
ATOM 7422 N GLY C 505 21.464 129.58641.391 1.00 89.86
7
ATOM 7423 CA GLY C 505 21.901 130.52842.403 1.00 90.42
6
ATOM 7424 C GLY C 505 23.341 130.11742.636 1.00 90.60
6
ATOM 7425 0 GLY C 505 24.243 130.94042.799 1.00 91.07
8
ATOM 7426 N ASP C 506 23.544 128.80742.646 1.00 82.76
7
ATOM 7427 CA ASP C 506 24.864 128.22942.806 1.00 81.90
6
ATOM 7428 CB ASP C 506 25.011 127.53844.159 1.00 67.53
6
ATOM 7429 CG ASP C 506 25.609 128.44245.207 1.00 66.91
6
ATOM 7430 OD1 ASP C 506 26.570 129.16244.876 1.00 66.91
8
ATOM 7431 OD2 ASP C 506 25.132 128.42746.361 1.00 66.05
8
ATOM 7432 C ASP C 506 25.026 127.21041.694 1.00 81.36
6
ATOM 7433 0 ASP C 506 24.542 126.08241.796 1.00 81.62
8
ATOM 7434 N ARG C 507 25.683 127.62440.617 1.00 58.94
7
ATOM 7435 CA ARG C 507 25.907 126.73539.493 1.00 57.88
6
ATOM 7436 CB ARG C 507 25.913 127.52838.176 1.00 68.18
6
ATOM 7437 CG ARG C 507 24.603 128.25937.838 1.00 67.43
6
ATOM 7438 CD ARG C 507 23.667 127.44736.925 1.00 66.82
6
ATOM 7439 NE ARG C 507 22.497 128.22436.484 1.00 66.76.
7
133

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WO 02/073192 PCT/US02/07535
ATOM 7440 CZ ARGC 507 21.711 127.902 35.452 1.00 66.55
6
ATOM 7441 NH1 ARGC 507 21.953 126.812 34.740 1.00 67.25
7
ATOM 7442 NH2 ARGC 507 20.686 128.679 35.114 1.00 66.00
7
ATOM 7443 C ARGC 507 27.267 126.094 39.726 1.00 57.63
6
ATOM 7444 0 ARGC 507 28.287 126.763 39.609 1.00 57.50
8
ATOM 7445 N ILEC 508 27.276 124.810 40.084 1.00 61.89
7
ATOM 7446 CA ILEC 508 28.528 124.088 40.314 1.00 62.24
6
ATOM 7447 CB ILEC 508 28.281 122.634 40.777 1.00 88.29
6
ATOM 7448 CG2 ILEC 508 29.423 122.182 41.678 1.00 87.84
6
ATOM 7449 CG1 ILEC 508 26.928 122.516 41.492 1.00 89.46
6
ATOM 7450 CD1 ILEC 508 26.782 123.365 42.747 1.00 92.22
6
ATOM 7451 C ILEC 508 29.201 124.047 38.950 1.00 62.15
6
ATOM 7452 O ILEC 508 28.743 123.347 38.046 1.00 62.11
8
ATOM 7453 N ALAC 509 30.287 124.791 38.789 1.00 52.75
7
ATOM 7454 CA ALAC 509 30.936 124.835 37.490 1.00 53.21
6
ATOM 7455 CB ALAC 509 30.547 126.118 36.771 1.00107.64
6
ATOM 7456 C ALAC 509 32.441 124.699 37.515 1.00 53.71
6
ATOM 7457 0 ALAC 509 33.103 124.973 38.522 1.00 53.78
8
ATOM 7458 N THRC 510 32.963 124.276 36.370 1.00 30.63
7
ATOM 7459 CA THRC 510 34.387 124.060 36.170 1.00 32.20
6
ATOM 7460 CB THRC 510 34.670 122.720 35.398 1.00 79.27
6
ATOM 7461 OG1 THRC 510 33.839 121.663 35.897 1.00 79.86
8
ATOM 7462 CG2 THRC 510 36.116 122.308 35.568 1.00 80.57
6
ATOM 7463 C THRC 510 34.898 125.202 35.304 1.00 33.62
6
ATOM 7464 0 THRC 510 34.344 125.480 34.241 1.00 33.24
8
ATOM 7465 N ASPC 511 35.938 125.879 35.769 1.00 82.32
7
ATOM 7466 CA ASPC 511 36.535 126.947 34.991 1.00 85.09
6
ATOM 7467 CB ASPC 511 36.118 128.327 35.514 1.00169.52
6
ATOM 7468 CG ASPC 511 36.831 128.715 36.783 1.00172.77
6
ATOM 7469 OD1 ASPC 511 36.832 127.907 37.733 1.00174.27
8
ATOM 7470 OD2 ASPC 511 37.384 129.834 36.830 1.00173.99
8
ATOM 7471 C ASPC 511 38.033 126.725 35.116 1.00 86.28
6
ATOM 7472 0 ASPC 511 38.574 126.629 36.218 1.00 .86.46
8
ATOM 7473 N ARGC 512 38.688 126.604 33.967 1.00 86.90
7
ATOM 7474 CA ARGC 512 40.122 126.357 33.889 1.00 87.82
6
ATOM 7475 CB ARGC 512 40.927 127.487 34.541 1.00 96.06
6
ATOM 7476 CG ARGC 512 42.398 127.386 34.195 1.00 96.74
6
ATOM 7477 CD ARGC 512 43.316 128.011 35.217 1.00 96.67
6
ATOM 7478 NE ARGC 512 44.680 127.551 34.979 1.00 96.37
7
ATOM 7479 CZ ARGC 512 45.720 127.823 35.757 1.00 96.51
6
ATOM 7480 NH1 ARGC 512 45.570 128.568 36.843 1.00 97.43
7
ATOM 7481 NH2 ARGC 512 46.912 127.332 35.451 1.00 95.66
7
ATOM 7482 C ARGC 512 40.482 125.039 34.561 1.00 88.16
6
ATOM 7483 O ARGC 512 40.539 123.993 33.915 1.00 88.76
8
ATOM 7484 N ALAC 513 40.722 125.096 35.865 1.00141.03
7
ATOM 7485 CA ALAC 513 41.086 123.908 36.616 1.00141.99
6
ATOM 7486 CB ALAC 513 42.563 123.963 36.978 1.00124.59
6
ATOM 7487 C ALAC 513 40.242 123.771 37.874 1.00141.81
6
ATOM 7488 0 ALAC 513 39.400 122.873 37.972 1.00141.21
8
ATOM 7489 N ALAC 514 40.478 124.663 38.834 1.00 56.61
7
ATOM 7490 CA ALAC 514 39.745 124.653 40.094 1.00 56.03
6
ATOM 7491 CB ALAC 514 39.916 125.989 40.800 1.00 86.46
6
ATOM 7492 C ALAC 514 38.260 124.367 39.871 1.00 56.39
6
ATOM 7493 0 ALAC 514 37.675 124.770 38.859 1.00 56.05
8
ATOM 7494 N ALAC 515 37.662 123.655 40.819 1.00 79.23
7
ATOM 7495 CA ALAC 515 36.248 123.320 40.753 1.00 80.00
6
134

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 7496 CB ALAC 515 35.969 122.106 41.635 1.00 13.87
ATOM 7497 C ALAC 515 35.475 124.530 41.263 1.00 80.71
ATOM 7498 O ALAC 515 34.437 124.386 41.905 1.00 81.84
ATOM 7499 N ARGC 516 35.989 -125.72040.960 1.00 51.67
ATOM 7500 CA ARGC 516 35.402 126.977 41.414 1.00 52.83
ATOM 7501 CB ARGC 516 35.864 128.127 40.508 1.00184.59
ATOM 7502 CG ARGC 516 37.372 128.365 40.645 1.00186.25
ATOM 7503 CD ARGC 516 37.860 129.694 40.076 1.00188.19
ATOM 7504 NE ARGC 516 39.271 129.915 40.405 1.00188.67
ATOM 7505 CZ ARGC 516 39.960 131.008 40.087 1.00188.92
ATOM 7506 NH1 ARGC 516 39.377 131.999 39.425 1.00188.66
ATOM 7507 NH2 ARGC 516 41.237 131.113 40.435 1.00187.95
ATOM 7508 C ARGC 516 33.892 127.000 41.620 1.00 53.89
ATOM 7509 0 ARGC 516 33.109 126.812 40.684 1.00 53.00
ATOM 7510 N ARGC 517 33.538 127.218 42.894 1.00 97.04
ATOM 7511 CA ARGC 517 32.179 127.293 43.460 1.00 99.09
ATOM 7512 CB ARGC 517 32.102 128.467 44.452 1.00153.60
ATOM 7513 CG ARGC 517 30.689 128.839 44.899 1.00154.88
ATOM 7514 CD ARGC 517 30.408 128.483 46.359 1.00156.22
ATOM 7515 NE ARGC 517 31.052 129.399 47.300 1.00156.32
ATOM 7516 CZ ARGC 517 30.759 129.473 48.598 1.00156.14
ATOM 751'7 NH1 ARGC 517 29.827 128.685 49.126 1.00155.63
ATOM 7518 NH2 ARGC 517 31.397 130.338 49.373 1.00156.15
ATOM 7519 C ARGC 517 31.014 127.388 42.501 1.00 99.92
ATOM 7520 0 ARGC 517 30.392 126.388 42.151 1.00 99.19
ATOM 7521 N ALAC 518 30.710 128.610 42.096 1.00151.09
ATOM 7522 CA ALAC 518 29.613 128.841 41.187 1.00154.07
ATOM 7523 CB ALAC 518 28.288 128.715 41.932 1.00 16.95
ATOM 7524 C ALAC 518 29.757 130.227 40.597 1.00156.60
ATOM 7525 O ALAC 518 29.198 131.186 41.124 1.00158.28
ATOM 7526 N GLYC 519 30.521 130.336 39.513 1.00153.30
ATOM 7527 CA GLYC 519 30.706 131.628 38.875 1.00155.29
ATOM 7528 C GLYC 519 29.359 132.307 38.758 1.00156.47
ATOM 7529 O GLYC 519 29.251 133.535 38.738 1.00156.88
ATOM 7530 N- ALAC 520 28.327 131.473 38.677 1.00125.80
ATOM 7531 CA ALAC 520 26.946 131.917 38.582 1.00127.15
ATOM 7532 CB ALAC 520 26.489 132.455 39.933 1.00108.64
ATOM 7533 C ALAC 520 26.659 132.950 37.494 1.00128.57
ATOM 7534 0 ALAC 520 25.733 133.753 37.644 1.00129.26
ATOM 7535 N PROC 521 27.425 132.938 36.378 1.00208.87
ATOM 7536 CD PROC 521 28.431 131.966 35.911 1.00138.40
ATOM 7537 CA PROC 521 27.154 133.928 35.330 1.00208.87
ATOM 7538 CB PROC 521 27.887 133.359 34.120 1.00137.39
ATOM 7539 CG PROC 521 29.061 132.689 34.740 1.00137.82
ATOM 7540 C PROC 521 25.657 134.027 35.098 1.00208.87
ATOM 7541 O PROC 521 25.151 135.052 34.643 1.00208.87
ATOM 7542 N ALAC 522 24.963 132.944 35.428 1.00126.01
ATOM 7543 CA ALAC 522 23.522 132.869 35.290 1.00125.89
ATOM 7544 CB ALAC 522 22.857 133.341 36.580 1.00182.72
ATOM 7545 C ALAC 522 23.029 133.693 34.100 1.00125.77
.
ATOM 7546 O ALAC 522 22.082 134.471 34.219 1.00125.75
ATOM 7547 N ALAC 523 23.689 133.530 32.954 1.00135.59
ATOM 7548 CA ALAC 523 23.308 134.246 31.737 1.00135.13
ATOM 7549 CB ALAC 523 24.499 134.348 30.776 1.00 81.38
ATOM 7550 C ALAC 523 22.161 133.503 31.070 1.00135.17
ATOM 7551 0 ALAC 523 21.694 133.912 30.009 1.00135.05
135

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 7552 N ALA C 524 21.728 132.410 31.702 1.00120.70
7
ATOM 7553 CA ALA C 524 20.631 131.582 31.208 1.00120.84
6
ATOM 7554 CB ALA C 524 19.303 132.271 31.483 1.00194.26
6
ATOM 7555 C ALA C 524 20.788 131.299 29.713 1.00120.68
6
ATOM 7556 O ALA C 524 21.040 130.160 29.309 1.00120.75
8
ATOM 7557 N ALA C 525 20.609 132.336 28.898 1.00141.28
7
ATOM 7558 CA ALA C 525 20.779 132.240 27.452 '1.00140.31
6
ATOM 7559 CB ALA C 525 20.321 133.532 26.791 1.00135.97
6
ATOM 7560 C ALA C 525 22.287 132.059 27.307 1.00139.91
6
ATOM 7561 O ALA C 525 23.009 132.243 28.287 1.00139.53
8
ATOM 7562 N PRO C 526 .22.788 131.771 26.089 1.00159.42
7
ATOM 7563 CD PRO C 526 22.311 132.434 24.863 1.00208.87
6
ATOM 7564 CA PRO C 526 24.231 131.581 25.970 1.00159.07
6
ATOM 7565 CB PRO C 526 24.677 132.935 25.461 1.00208.87
6
ATOM 7566 CG PRO C 526 23.575 133.212 24.394 1.00208.87
6
ATOM 7567 C PRO C 526 24.881 131.136 27.273 1.00158.57
6
ATOM 7568 0 PRO C 526 25.861 131.715 27.732 1.00159.08
8
ATOM 7569 N ALA C 527 24.287 130.095 27.854 1.00119.34
7
ATOM 7570 CA ALA C 527 24.705 129.485 29.113 1.00118.20
6
ATOM 7571 CB ALA C 527 24.608 127.986 28.990 1.00 51.31
6
ATOM 7572 C ALA C 527 26.107 129.872 29.563 1.00117.87
6
ATOM 7573 O ALA C 527 27.074 129.546 28.881 1.00118.65
8
ATOM 7574 N ALA C 528 26.206 130.545 30.714 1.00187.90
7
ATOM 7575 CA ALA C 528 27.488 130.992 31.273 1.00186.63
6
ATOM 7576 CB ALA C 528 27.687 130.398 32.677 1.00 25.37
6
ATOM 7577 C ALA C 528 28.638 130.583 30.358 1.00186.26
6
ATOM 7578 O ALA C 528 29.355 129.626 30.646 1.00186.75
8
ATOM 7579 N ALA C 529 28.798 131.312 29.254 1.00208.87
7
ATOM 7580 CA ALA C 529 29.829 131.018 28.259 1.00208.87
6
ATOM 7581 CB ALA C 529 31.159 130.779 28.936 1.00108.91
6
ATOM 7582 C ALA C 529 29.386 129.768 27.498 1.00208.87'
6
ATOM 7583 O ALA C 529 28.406 129.810 26.756 1.00208.87
8
ATOM 7584 N ALA C 530 30.106 128.663 27.676 1.00 84.61
7
ATOM 7585 CA ALA C 530 29.747 127.402 27.033 1.00 80.94
6
ATOM 7586 CB ALA C 530 30.852 126.936 26.058 1.00 32.73
6
ATOM 7587 C ALA C 530 29.536 126.378 28.146 1.00 78.57
6
ATOM 7588 O ALA C 530 29.626 125.177 27.918 1.00 78.56
8
ATOM 7589 N ALA C 531 29.252 126.877 29.351 1.00 55.15
7
ATOM 7590 CA ALA C 531 29.011 126.049 30.535 1.00 52.08
6
ATOM 7591 CB ALA C 531 28.402 126.892 31.633 1.00 68.22
6
ATOM 7592 C ALA C 531 28.098 124.878 30.236 1.00 50.47
6
ATOM 7593 O ALA C 531 27.437 124.863 29.208 1.00 50.11
8
ATOM 7594 N MET C 532 28.050 123.911 31.147 1.00 83.34
7
ATOM 7595 CA MET C 532 27.218 122.716 30.980 1.00 82.40
6
ATOM 7596 CB MET C 532 25.903 123.054 30.258 1.00107.38
6
ATOM 7597 CG MET C 532 24.800 123.637 31.124 1.00108.62
6
ATOM 7598 SD MET C 532 23.914 122.374 32.061 1.00111.01
16
ATOM 7599 CE MET C 532 22.767 121.732 30.820 1.00108.74
6
ATOM 7600 C MET C 532 27.920 121.596 30.205 1.00 80.11
6
ATOM 7601 0 MET C 532 28.729 121.845 29.304 1.00 79.97
8
ATOM 7602 N ASP C 533 27.597 120.358 30.561 1.00 45.22
7
ATOM 7603 CA ASP C 533 28.167 119.201 29.894 1.00 42.94
6
ATOM 7604 CB ASP C 533 29.153 118.488 30.815 1.00118.04
6
ATOM 7605 CG ASP C 533 30.377 119.314 31.092 1.00119.82
6
ATOM 7606 OD1 ASP C 533 30.962 119.851 30.126 1.00120.83
8
ATOM 7607 OD2 ASP C 533 30.758 119.421 32.274 1.00120.99
8
136

CA 02440409 2003-09-09
WO 02/073192 PCT/US02/07535
ATOM 7608 C ASP C 533 27.080 118.227 29.470 1.00 40.69
ATOM 7609 O ASP C 533 26.354 117.706 30.304 1.00 39.20
ATOM 7610 N VAL C 534 26.965 117.987 28.170 1.00 45.56
ATOM 7611 CA VAL C 534 25.972 117.052 27.673 1.00 43.30
ATOM 7612 CB VAL C 534 26.208 116.733 26.180 1.00 56.00
ATOM 7613 CG1 VAL C 534 24.884 116.584 25.472 1.00 57.40
ATOM 7614 CG2 VAL C 534 27.060 117.801 25.536 1.00 55.70
ATOM 7615 C VAL C 534 26.176 115.768 28.479 1.00 41.63
ATOM 7616 0 VAL C 534 27.102 115.012 28.200 1.00 42.30
ATOM 7617 N SER C 535 25.336 115.516 29.480 1.00 31.85
ATOM 7618 CA SER C 535 25.480 114.314 30.315 1.00 30.44
ATOM 7619 CB SER C 535 25.198 113.049 29.505 1.00 52.78
ATOM- 7620 OG SER C 535 23.802 112.782 29.432 1.00 53.48
ATOM 7621 C SER C 535 26.837 114.172 30.991 1.00 29.50
ATOM 7622 O SER C 535 27.797 114.846 30.651 1.00 29.54
ATOM 7623 N PRO C 536 26.920 113.298 31.985 1.00 57.81
ATOM 7624 CD PRO C 536 25.784 112.903 32.819 1.00 35.93
ATOM 7625 CA PRO C 536 28.163 113.074 32.709 1.00 57.22
ATOM 7626 CB PRO C 536 27.685 112.636 34.082 1.00 35,.52
ATOM 7627 CG PRO C 536 26.313 113.204 34.175 1.00 35.98
ATOM 7628 C PRO C 536 29.005 112.003 32.058 1.00 57.09
ATOM 7629 O PRO C 536 30.212 111.962 32.257 1.00 58.55
ATOM 7630 N LYS C 537 28.365 111.127 31.294 1.00 66.52
ATOM 7631 CA LYS C 537 29.072 110.045 30.620 1.00 64.77
ATOM 7632 CB LYS C 537 28.204 109.472 29.504 1.00 67.06
ATOM 7633 CG LYS C 537 26.778 109.233 29.915 1.00 69.21
ATOM 7634 CD LYS C 537 25.935 108.782 28.744 1.00 71.51
ATOM 7635 CE LYS C 537 24.456 108.743 29.123 1.00 73.76
ATOM 7636 NZ LYS C 537 24.158 107.832 30.274 1.00 76.52
ATOM 7637 C LYS C 537 30.382 110.539 30.018 1.00 63.46
ATOM 7638 O LYS C 537 31.406 109.853 30.085 1.00 65.23
ATOM 7639 N GLN C 538 30.340 111.738 29.443 1.00 14.62
ATOM 7640 CA GLN C 538 31.497 112.314 28.794 1.00 13.87
ATOM 7641 CB GLN C 538 31.140 113.679 28.237 1.00 29.72
ATOM 7642 CG GLN C 538 30.595 114.627 29.268 1.00 28.74
ATOM 7643 CD GLN C 538 30.412 116.020 28.710 1.00 26.62
ATOM 7644 OE1 GLN C 538 31.361 116.639 28.232 1.00 26.40
ATOM 7645 NE2 GLN C 538 29.189 116.522 28.766 1.00 25.10
ATOM 7646 C GLN C 538 32.741 112.413 29.669 1.00 13.87
ATOM 7647 0 GLN C 538 33.836 112.658 29.175 1.00 14.40
ATOM 7648 N VAL C 539 32.598 112.238 30.969 1.00 61.78
ATOM 7649 CA VAL C 539 33.766 112.294 31.827 1.00 62.62
ATOM 7650 CB VAL C 539 33.397 112.686 33.274 1.00 42.82
ATOM 7651 CG1 VAL C 539 34.640 112.726 34.142 1.00 43.04
ATOM 7652 CG2 VAL C 539 32.720 114.012 33.289 1.00 43.33
ATOM 7653 C VAL C 539 34.295 110.878 31.857 1.00 64.28
ATOM 7654 O VAL C 539 35.237 110.578 32.583 1.00 67.03
ATOM 7655 N PHE C 540 33.697 109.997 31.064 1.00 55.62
ATOM 7656 CA PHE C 540 34.122 108.617 31.128 1.00 55.60
ATOM 7657 CB PHE C 540 33.207 107.879 32.120 1.00 28.30
ATOM 7658 CG PHE C 540 33.315 108.391 33.538 1.00 24.65
ATOM 7659 CD1 PHE C 540 32.633 109.518 33.935 1.00 22.13
ATOM 7660 CD2 PHE C 540 34.169 107.797 34.439 1.00 25.34
ATOM 7661 CE1 PHE C 540 32.809 110.044 35.188 1.00 22.70
ATOM 7662 CE2 PHE C 540 34.344 108.325 35.697 1.00 26.61
ATOM 7663 CZ PHE C 540 33.665 109.448 36.067 1.00 25.32
137

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ATOM 7664 C PHE C 540 34.271 107.803 29.846 1.00 56.85
6
ATOM 7665 0 PHE C 540 33.837 108.214 28.764 1.00 57.37
8
ATOM 7666 N SER C 541 34.913 106.641 30.009 1.00 50.86
7
ATOM 7667 CA SER C 541 35.186 105.669 28.952 1.00 49.99
6
ATOM 7668 CB SER C 541 36.327 104.781 29.393 1.00 61.30
6
ATOM 7669 OG SER C 541 36.199 104.522 30.780 1.00 65.14
8
ATOM 7670 C SER C 541 33.971 104.800 28.664 1.00 48.71
6
ATOM 7671 O SER C 541 33.198 104.473 29.563 1.00 48.33
8
ATOM 7672 N LEU C 542 33.835 104.389 27.409 1.00 55.24
7
ATOM 7673 CA LEU C 542 32.697 103.590 26.976 1.00 53.98
6
ATOM 7674 CB LEU C 542 32.870 103.132 25.533 1.00 17.92
6
ATOM 7675 CG LEU C 542 31.622 102.457 24.973 1.00 15.59
6
ATOM 7676 CD1 LEU C 542 30.413 103.338 25.206 1.00 15.21
6
ATOM 7677 CD2 LEU C 542 31.804 102.196 23.501 1.00 15.84
6
ATOM 7678 C LEU C 542 32.334 102.386 27.811 1.00 54.34
6
ATOM 7679 O LEU C 542 31.156 .102.13128.010 1.00 55.12
8
ATOM 7680 N ASN C 543 33.309 101.622 28.291 1.00 39.01
7
ATOM 7681 CA ASN C 543 32.926 100.467 29.086 1.00 39.30
6
ATOM 7682 CB ASN C 543 33.774 99.207 28.743 1.00 35.95
6
ATOM 7683 CG ASN C 543 35.259 99.387 28.936 1.00 36.82
6
ATOM 7684 OD1 ASN C 543 35.756 100.492 29.145 1.00 37.92
8
ATOM 7685 ND2 ASN C 543 35.985 98.284 28.844 1.00 35.25
7
ATOM 7686 C ASN C 543 32.826 100.718 30.583 1.00 38.44
6
ATOM 7687 0 ASN C 543 32.380 99.850 31.331 1.00 39.50
8
ATOM 7688 N THR C 544 33.211 101.905 31.034 1.00 57.02
7
ATOM 7689 CA THR C 544 33.058 102.184 32.451 1.00 56.20
6
ATOM 7690 CB THR C 544 33.889 103.384 32.944 1.00 23.10
6
ATOM 7691 OG1 THR C 544 35.242 102.966 33.159 1.00 21.43
8
ATOM 7692 CG2 THR C 544 33.332 103.924 34.261 1.00 20.88
6
ATOM 7693 C THR C 544 31.601 102.529 32.580 1.00 57.34
6
ATOM 7694 0 THR C 544 30.917 102.020 33.457 1.00 57.84
8
ATOM 7695 N ASN C 545 31.117 103.374 31.678 1.00 42.11
7
ATOM 7696 CA ASN C 545 29.720 103.776 31.713 1.00 43.29
6
ATOM 7697 CB ASN C 545 29.273 104.314 30.353 1.00 52.15
6
ATOM 7698 CG ASN C 545 29.957 105.617 29.977 1.00 53.97
6
ATOM 7699 OD1 ASN C 545 29.700 106.662 30.566 1.00 54.69
8
ATOM 7700 ND2 ASN C 545 30.835 105.556 28.988 1.00 54.19
7
ATOM 7701 C ASN C 545 28.845 102.592 32.091 1.00 44.50
6
ATOM 7702 0 ASN C 545 27.907 102.731 32.870 1.00 46.05
8
ATOM 7703 N LEU C 546 29.179 101.420 31.561 1.00 60.59
7
ATOM 7704 CA LEU C 546 28.403 100.213 31.812 1.00 60.11
6
ATOM 7705 CB LEU C 546 28.992 99.035 31.020 1.00 37.83
6
ATOM 7706 CG LEU C 546 29.088 99.200 29.493 1.00 37.99
6
ATOM 7707 CD1 LEU C 546 29.306 97.862 28.830 1.00 37.92
6
ATOM 7708 CD2 LEU C 546 27.821 99.807 28.958 1.00 37.51
6
ATOM 7709 C LEU C 546 28.232 99.818 33.27 1.00 59.65
4 6
ATOM 7710 O LEU C 546 27.836 98.693 33.558 1.00 61.57
8
ATOM 7711 N ILE C 547 28.512 100.738 34.195 1.00 28.92
7
ATOM 7712 CA ILE C 547 28.377 100.475 35.637 1.00 27.88
6
ATOM 7713 CB ILE C 547 29.623 100.935 36.422 1.00 54.96
6
ATOM 7714 CG2 ILE C 547 29.344 100.847 37.900 1.00 56.07
6
ATOM 7715 CG1 ILE C 547 30.831 100.062 36.074 1.00 54.53
6
ATOM 7716 CD1 ILE C 547 32.127 100.537 36.711 1.00 53.36
6
ATOM 7717 C ILE C 547 27.160 101.205 36.211 1.00 26.96
6
ATOM 7718 0 ILE C 547 27.216 102.399 36.485 1.00 25.95
8
ATOM 7719 N PRO C 548 26.065 100.473 36.451 1.00 55.58
7
138

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ATOM 7720 CD PRO C 548 26.037 99.021 36.671 1.00 35.60
ATOM 7721 CA .PROC 548 24.846 101.06336.977 1.00 55.04
ATOM 7722 CB PRO C 548 23.954 99.849 37.190 1.00 33.94
ATOM 7723 CG PRO C 548 24.900 98.866 37.658 1.00 34.54
ATOM 7724 C PRO C 548 25.049 101.89738.222 1.00 55.38
ATOM 7725 O PRO C 548 24.279 102.81938.473 1.00 57.14
ATOM 7726 N PHE C 549 26.061 101.61139.022 1.00 32.51
ATOM 7727 CA PHE C 549 26.220 102.46740.191 1.00 33.20
ATOM 7728 CB PHE C 549 25.645 101.79841.446 1.00 27.42
ATOM 7729 CG PHE C 549 24.130 101.79341.503 1.00 24.30
ATOM 7730 CD1 PHE C 549 23.387 100.84040.798 1.00 23.85
ATOM 7731 CD2 PHE C 549 23.449 102.70842.295 1.00 22.95
ATOM 7732 CE1 PHE C 549 21.989 100.79540.889 1.00 22.47
ATOM 7733 CE2 PHE C 549 22.058 102.66542.387 1.00 23.27
ATOM 7734 CZ PHE C 549 21.331 101.70441.682 1.00 22.31
ATOM 7735 C PHE C 549 27.650 102.94140.445 1.00 33.28
ATOM 7736 0 PHE C 549 28.181 102.81141.549 1.00 33.61
ATOM 7737 N LEU C 550 28.252 103.52539.412 1.00 29.32
ATOM 7738 CA LEU C 550 29.619 104.02539.485 1.00 29.00
ATOM 7739 CB LEU C 550 30.009 104.69638.156 1.00 46.50
ATOM 7740 CG LEU C 550 31.458 105.14037.930 1.00 45.08
ATOM 7741 CD1 LEU C 550 32.372 103.94037.982 1.00 46.72
ATOM 7742 CD2 LEU C 550 31.594 105.83336.586 1.00 43.39
ATOM 7743 C LEU C 550 29.723 105.01840.621 1.00 29.29
ATOM 7744 O LEU C 550 30.572 104.86441.492 1.00 27.19
ATOM 7745 N GLU C 551 28.842 106.01940.613 1.00 28.00
ATOM 7746 CA GLU C 551 28.824 107.07841.627 1.00 30.78
ATOM 7747 CB GLU C 551 27.403 107.61141.844 1.00103.26
ATOM 7748 CG GLU C 551 26.502 107.57040.629 1.00106.00
ATOM 7749 CD GLU C 551 25.687 106.30240.575 1.00108.97
ATOM 7750 OE1 GLU C 551 26.287 105.21340.571 1.00108.75
ATOM 7751 OE2 GLU C 551 24.443 106.39240.544 1.00110.84
ATOM 7752 C GLU C 551 29.395 106.66642.978 1.00 31.42
ATOM 7753 0 GLU C 551 30.171 107.40343.564 1.00 31.29
ATOM 7754 N HIS C 552 29.010 105.49143.466 1.00 50.43
ATOM 7755 CA HIS C 552 29.490 105.00144.754 1.00 51.46
ATOM 7756 CB HIS C 552 28.470 104.04745.341 1.00 54.12
ATOM 7757 CG HIS C 552 27.067 104.54445.220 1.00 54.38
ATOM 7758 CD2 HIS C 552 26.123 104.31244.276 1.00 55.28
ATOM 7759 ND1 HIS C 552 26.505 105.42846.115 1.00 54.20
ATOM 7760 CE1 HIS C 552 25.274 105.71545.728 1.00 55.46
ATOM 7761 NE2 HIS C 552 25.019 105.05444.614 1.00 55.94
ATOM 7762 C HIS C 552 30.830 104.30644.599 1.00 51.10
ATOM 7763 0 HIS C 552 31.788 104.63245.288 1.00 53.17
ATOM 7764 N ASP C 553 30.893 103.33743.701 1.00 52.36
ATOM 7765 CA ASP C 553 32.134 102.62943.435 1.00 51.21
ATOM 7766 CB ASP C 553 32.087 102.02642.036 1.00 32.32
ATOM 7767 CG ASP C 553 31.310 100.75041.982 1.00 32.47
ATOM 7768 OD1 ASP C 553 30.513 100.47842.896 1.00 33.28
ATOM 7769 OD2 ASP C 553 31.498 100.01741.006 1.00 31.58
ATOM 7770 C ASP C 553 33.331 103.57643.504 1.00 50.90
ATOM 7771 O ASP C 553 33.383 104.58042.786 1.00 51.83
ATOM 7772 N ASP C 554 34.290 103.25144.367 1.00 33.65
ATOM 7773 CA ASP C 554 35.501 104.05044.509 1.00 31.93
ATOM 7774 CB ASP C 554 36.383 103.47545.612 1.00 68.27
ATOM 7775 CG ASP C 554 37.684 104.21845.756 1.00 72.84
139

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ATOM 7776 OD1 ASP C 554 38.411 104.32044.749 1.00 73.81
8
ATOM 7777 OD2 ASP C 554 37.984 104.69746.872 1.00 76.46
8
ATOM 7778 C ASP C 554 36.224 103.97143.181 1.00 30.26
6
ATOM 7779 O ASP C 554 36.417 102.88142.639 1.00 29.04
8
ATOM 7780 N ALA C 555 36.608 105.13042.653 1.00 44.51
7
ATOM 7781 CA ALA C 555 37.301 105.20441.370 1.00 42.66
6
ATOM 7782 CB ALA C 555 38.172 106.45941.315 1.00 29.64
6
ATOM 7783 C ALA C 555 38.146 103.95641.128 1.00 41.50
6
ATOM 7784 O ALA C 555 37.787 103.10740.318 1.00 41.76
8
ATOM 7785 N ASN C 556 39.254 103.84241.853 1.00 29.75
7
ATOM 7786 CA ASN C 556 40.163 102.70541.735 1.00 29.63
6
ATOM 7787 CB ASN C 556 41.039 102.61442.984 1.00 56.62
6
ATOM 7788 CG ASN C 556 42.286 101.78942.758 1.00 57.90
6
ATOM 7789 OD1 ASN C 556 42.215 100.62242.368 1.00 58.43
8
ATOM 7790 ND2 ASN C 556 43.443 102.39543.000 1.00 57.43
7
ATOM 7791 C ASN C 556 39.482 101.35141.500 1.00 27.87
6
ATOM 7792 O ASN C 556 39.574 100.77840.417 1.00 26.94
8
ATOM 7793 N ARG C 557 38.807 100.83142.515 1.00 65.96
7
ATOM 7794 CA ARG C 557 38.147 99.544 42.367 1.00 64.43
6
ATOM 7795 CB ARG C 557 37.297 99.222 43.582 1.00 26.99
6
ATOM 7796 CG ARG C 557 37.075 97.758 43.729 1.00 24.27
6
ATOM 7797 CD ARG C 55'7 38.387 97.118 44.108 1.00 22.59
6
ATOM 7798 NE ARG C 557 38.365 95.665 44.016 1.00 22.25
7
ATOM 7799 CZ ARG C 557 39.348 94.890 44.455 1.00 22.39
6
ATOM 7800 NH1 ARG C 557 40.414 95.442 45.019 1.00 22.25
7
ATOM 7801 NH2 ARG C 557 39.283 93.573 44.309 1.00 22.91
7
ATOM 7802 C ARG C 557 37.253 99.524 41.144 1.00 64.64
6
ATOM 7803 0 ARG C 557 37.111 98.495 40.491 1.00 66.31
8
ATOM 7804 N ALA C 558 36.634 100.66140.847 1.00 19.66
7
ATOM 7805 CA ALA C 558 35.756 100.75639.690 1.00 18.12
6
ATOM 7806 CB ALA C 558 35.153 102.15939.615 1.00 46.30
6
ATOM 7807 C ALA C 558 36.490 100.41338.380 1.00 17.77
6
ATOM 7808 O ALA C 558 35.847 100.00337.411 1.00 15.92
8
ATOM 7809 N LEU C 559 37.824 100.57938.358 1.00 13.87
7
ATOM 7810 CA LEU C 559 38.633 100.28437.167 1.00 13.87
6
ATOM 7811 CB LEU C 559 40.013 100.98637.209 1.00 13.87
6
ATOM 7812 CG LEU C 559 41.269 100.50337.978 1.00 14.77
6
ATOM 7813 CD1 LEU C 559 41.522 99.032 37.759 1.00 14.05
6
ATOM 7814 CD2 LEU C 559 42.504 101.28937.513 1.00 13.87
6
ATOM 7815 C LEU C 559 38.822 98.781 37.046 1.00 13.87
6
ATOM 7816 O LEU C 559 39.047 98.256 35.960 1.00 15.26
8
ATOM 7817 N MET C 560 38.754 98.082 38.171 1.00 13.87
7
ATOM 7818 CA MET C 560 38.890 96.637 38.140 1.00 13.87
6
ATOM 7819 CB MET C 560 39.149 96.092 39.532 1.00 24.02
6
ATOM 7820 CG MET C 560 40.602 95.859 39.795 1.00 24.05
6
ATOM 7821 SD MET C 560 40.804 95.282 41.440 1.00 24.44
16
ATOM 7822 CE MET C 560 41.402 96.799 42.230 1.00 24.05
6
ATOM 7823 C MET C 560 37.625 96.021 37.576 1.00 13.87
6
ATOM 7824 0 MET C 560 37.547 94.817 37.365 1.00 13.87
8
ATOM 7825 N GLY C 561 36.627 96.862 37.350 1.00 28.68
7
ATOM 7826 CA GLY C 561 35.384 96.393 36.781 1.00 29.22
6
ATOM 7827 C GLY C 561 35.362 96.946 35.375 1.00 30.05
6
ATOM 7828 O GLY C 561 35.040 96.258 34.413 1.00 30.74
8-
ATOM 7829 N SER C 562 35.710 98.216 35.258 1.00 21.52
7
ATOM 7830 CA SER C 562 35.746 98.842 33.965 1.00 23.76
6
ATOM 7831 CB SER C 562 36.439 100.19734.076 1.00 50.05
6
140

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ATOM 7832 OG SERC 562 36.642 100.776 32.800 1.00 54.47 8
ATOM 7833 C SERC 562 36.522 97.901 33.041 1.00 23.13 6
ATOM 7834 0 SERC 562 36.001 97.465 32.014 1.00 23.33 8
ATOM 7835 N ASNC 563 37.753 97.563 33.423 1.00 14.06 7
ATOM 7836 CA ASNC 563 38.593 96.674 32.617 1.00 14.75 6
ATOM 7837 CB ASNC 563 40.015 96.557 33.186 1.00 59.25 6
ATOM 7838 CG ASNC 563 40.754 97.873 33.222 1.00 63.28 6
ATOM 7839 OD1 ASNC 563 40.354 98.848 32.588 1.00 67.18 8
ATOM 7840 ND2 ASNC 563 41.857 97.902 33.959 1.00 63.33 7
ATOM 7841 C ASNC 563 38.006 95.285 32.598 1.00 13.87 6
ATOM 7842 O ASNC 563 37.452 94.842 31.617 1.00 13.87 8
ATOM 7843 N METC 564 38.161 94.606 33.716 1.00 17.31 7
ATOM 7844 CA METC 564 37.688 93.262 33.895 1.00 16.52 6
ATOM 7845 CB METC 564 37.319 93.087 35.333 1.00 26.95 6
ATOM 7846 CG METC 564 36.910 91.729 35.687 1.00 28.06 6
ATOM 7847 SD METC 564 36.957 91.733 37.424 1.00 31.38 16
ATOM 7848 CE METC 564 38.697 91.983 37.652 1.00 32.35 6
ATOM 7849 C METC 564 36.507 92.920 33.032 1.00 16.05 6
ATOM 7850 O METC 564 36.483 91.892 32.381 1.00 16.44 8
ATOM 7851 N GLNC 565 35.509 93.778 33.045 1.00 26.07 7
ATOM 7852 CA GLNC 565 34.311 93.569 32.253 1.00 26.11 6
ATOM 7853 CB GLNC 565 33.591 94.916 32.178 1.00 18.22 6
ATOM 7854 CG GLNC 565 32.342 94.971 31.365 1.00 19.82 6
ATOM 7855 CD GLNC 565 31.359 95.923 31.975 1.00 20.65 6
ATOM 7856 OE1 GLNC 565 31.736 96.987 32.462 1.00 19.74 8
ATOM 7857 NE2 GLNC 565 30.086 95.551 31.956 1.00 20.72 7
ATOM 7858 C GLNC 565 34.670 93.011 30.859 1.00 25.51 6
ATOM 7859 O GLNC 565 34.077 92.049 30.383 1.00 24.62 8
ATOM 7860 N THRC 566 35.677 93.612 30.235 1.00 24.10 7
ATOM 7861 CA THRC 566 36.160 93.219 28.909 1.00 23.98 6
ATOM 7862 CB THRC 566 37.506 93.906 28.548 1.00 20.36 6
ATOM 7863 OG1 THRC 566 37.422 95.326 28.720 1.00 20.36 8
ATOM 7864 CG2 THRC 566 37.856 93.608 27.122 1.00 20.36 6
ATOM 7865 C THRC 566 36.435 91.731 28.805 1.00 24.17 6
ATOM 7866 O THRC 566 36.010 91.071 27.870 1.00 24.04 8
ATOM 7867 N GLNC 567 37.187 91.226 29.769 1.00 13.87 7
ATOM 7868 CA GLNC 567 37.573 89.834 29.809 1.00 13.87 6
ATOM 7869 CB GLNC 567 38.576 89.630 30.941 1.00 44.07 6
ATOM 7870 CG GLNC 567 39.702 90.652 30.918 1.00 47.21 6
ATOM 7871 CD GLNC 567 40.555 90.643 32.173 1.00 49.95 6
ATOM 7872 OE1 GLNC 567 40.059 90.818 33.285 1.00 50.29 8
ATOM 7873 NE2 GLNC 567 41.852 90.451 31.996 1.00 51.77 7
ATOM 7874 C GLNC 567 36.394 88.894 29.967 1.00 13.87 6
ATOM 7875 O GLNC 567 36.579 87.756 30.369 1.00 13.87 8
ATOM 7876 N ALAC 568 35.194 89.381 29.644 1.00 28.36 7
ATOM 7877 CA ALAC 568 33.937 88.620 29.728 1.00 28.85 6
ATOM 7878 CB ALAC 568 32.827 89.392 29.043 1.00 75.57 6
ATOM 7879 C ALAC 568 34.004 87.207 29.148 1.00 31.00'6
ATOM 7880 O ALAC 568 34.664 86.351 29.715 1.00 32.02 8
ATOM 7881 N VALC 569 33.305 86.974 28.033 1.00 22.44 7
ATOM 7882 CA VALC 569 33.259 85.672 27.332 1.00 24.38 6
ATOM 7883 CB VALC 569 34.054 84.547 28.049 1.00 38.29 6
ATOM 7884 CG1 VALC 569 33.295 84.039 29.253 1.00 39.66 6
ATOM 7885 CG2 VALC 569 34.321 83.413 27.085 1.00 39.89 6
ATOM 7886 C VALC 569 31.831 85.164 27.129 1.00 25.75 6
ATOM 7887 0 VALC 569 31.252 84.512 27.996 1.00 25.33 8
141

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ATOM 7888 N PRO C 570 31.265 85.427 25.948 1.00 29.95
ATOM 7889 CD PRO C 570 32.055 85.698 24.740 1.00 50.59
ATOM 7890 CA PRO C 570 29.908 85.027 25.579 1.00 31.13
ATOM 7891 CB PRO C 570 29.953 85.013 24.055 1.00 50.91
ATOM 7892 CG PRO C 57G , 31.394 84.798 23.750 1.00 50.20
ATOM 7893 C PRO C 570 29.480 83.694 26.138 1.00 31.83
ATOM 7894 O PRO C 570 30.207 82.707 26.024 1.00 31.32
ATOM 7895 N LEU C 571 28.292 83.686 26.736 1.00 31.25
ATOM 7896 CA LEU C 571 27.708 -82.48727.320 1.00 32.79
ATOM 7897 CB LEU C 571 27.247 82.729 28.764 1.00 13.87
ATOM 7898 CG LEU C 571 28.097 83.181 29.952 1.00 13.87
ATOM 7899 CD1 LEU C 571 28.730 84.518 29.670 1.00 13.87
ATOM 7900 CD2 LEU C 571 27.205 83.294 31.181 1.00 13.87
ATOM 7901 C LEU C 571 26.469 82.104 26.510 1.00 36.27
ATOM 7902 0 LEU C 571 26.038 82.839 25.613 1.00 35.73
ATOM 7903 N ILE C 572 25.907 80.947 26.851 1.00 37.31
ATOM 7904 CA ILE C 572 24.691 80.430 26.233 1.00 40.16
ATOM 7905 CB ILE C 572 24.666 78.898 26.248 1.00 48.31
ATOM 7906 CG2 ILE C 572 23.847 78.371 25.105 1.00 49.64
ATOM 7907 CG1 ILE C 572 26.078 78.357 26.099 1.00 50.85
ATOM 7908 CD1 ILE C 572 26.167 76.917 26.439 1.00 53.55
ATOM 7909 C ILE C 572 23.626 80.906 27.206 1.00 42.12
ATOM 7910 0 ILE C 572 23.315 80.207 28.175 1.00 44.93
ATOM 7911 N ARG C 573 23.090 82.097 26.952 1.00 47.21
ATOM 7912 CA ARG C 573 22.064 82.732 27.791 1.00 48.00
ATOM 7913 CB ARG C 573 21.563 81.804 28.904 1.00177.52
ATOM 7914 CG ARG C 573 20.346 80.973 28.541 1.00182.92
ATOM 7915 CD ARG C 573 20.107 79.866 29.558 1.00188.88
ATOM 7916 NE ARG C 573 18.780 79.269 29.433 1.00196.42
ATOM 7917 CZ ARG C 573 18.253 78.823 28.295 1.00201.00
ATOM 7918 NH1 ARG C 573 18.936 78.901 27.160 1.00202.35
ATOM 7919 NH2 ARG C 573 17.036 78.293 28.292 1.00203.74
ATOM 7920 C ARG C 573 22.711 83.940 28.410 1.00 45.92
ATOM 7921 0 ARG C 573 22.941 83.986 29.612 1.00 44.53
ATOM 7922 N ALA C 574 22.990 84.926 27.575 1.00 36.56
ATOM 7923 CA ALA C 574 23.655 86.123 28.026 1.00 36.57
ATOM 7924 CB ALA C 574 24.060 86.932 26.848 1.00 28.73
ATOM 7925 C ALA C 574 22.884 86.994 29.000 1.00 36.13
ATOM 7926 0 ALA C 574 23.401 87.345 30.057 1.00 37.99
ATOM 7927 N GLN C 575 21.652 87.350 28.663 1.00 22.89
ATOM 7928 CA GLN C 575 20.853 88.218 29.535 1.00 20.60
ATOM 7929 CB GLN C 575 20.633 87.599 30.921 1.00 41.70
ATOM 7930 CG GLN C 575 20.953 86.125 31.064 1.00 41.79
ATOM 7931 CD GLN C 575 20.832 85.685 32.491 1.00 41.32
ATOM 7932 OE1 GLN C 575 21.412 86.300 33.374 1.00 42.31
ATOM 7933 NE2 GLN C 575 20.074 84.627 32.732 1.00 40.07
ATOM 7934 C GLN C 575 21.542 89.557 29.756 1.00 19.44
ATOM 7935 0 GLN C 575 22.702 89.609 30.144 1.00 19.35
ATOM 7936 N ALA C 576 20.836 90.648 29.514 1.00 19.52
ATOM 7937 CA ALA C 576 21.432 91.947 29.749 1.00 19.48
ATOM 7938 .CB ALA C 576 20.508 93.042 29.267 1.00 59.31
ATOM 7939 C ALA C 576 21.554 92.006 31.252 1.00 19.91
ATOM 7940 0 ALA C 576 21.283 91.029 31.937 1.00 19.51
ATOM 7941 N PRO C 577 22.007 93.128 31.791 1.00 59.79
ATOM 7942 CD PRO C 577 22.962 94.057 31.168 1.00 54.76
ATOM 7943 CA PRO C 577 22.100 93.167 X3.255 1.00 61.36
142

DEMANDE OU BREVET VOLUMINEUX
LA PRESENTE PARTIE DE CETTE DEMANDE OU CE BREVET COMPREND
PLUS D'UN TOME.
CECI EST LE TOME 1 DE 3
~~ TTENANT LES PAGES 1 A 224
NOTE : Pour les tomes additionels, veuillez contacter 1e Bureau canadien des
brevets
JUMBO APPLICATIONS/PATENTS
THIS SECTION OF THE APPLICATION/PATENT CONTAINS MORE THAN ONE
VOLUME
THIS IS VOLUME 1 OF 3
CONTAINING PAGES 1 TO 224
NOTE: For additional volumes, please contact the Canadian Patent Office
NOM DU FICHIER / FILE NAME
NOTE POUR LE TOME / VOLUME NOTE:

?2440409 Summary - Canadian Patents Database (2024)
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